DE507591T1 - Anionenaustauschtrennung von nukleinsaeuren. - Google Patents

Anionenaustauschtrennung von nukleinsaeuren.

Info

Publication number
DE507591T1
DE507591T1 DE199292302930T DE92302930T DE507591T1 DE 507591 T1 DE507591 T1 DE 507591T1 DE 199292302930 T DE199292302930 T DE 199292302930T DE 92302930 T DE92302930 T DE 92302930T DE 507591 T1 DE507591 T1 DE 507591T1
Authority
DE
Germany
Prior art keywords
solid
solvent
concentration
contacting
anion exchange
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE199292302930T
Other languages
English (en)
Inventor
Will El Cerrito Ca 94530 Bloch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Publication of DE507591T1 publication Critical patent/DE507591T1/de
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8804—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 automated systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8827—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Claims (1)

  1. 050759
    92.302930.G
    Patentansprüche :
    1. Wäßriges Lösungsmittel, welches für die Anionenaustauscherchromatograpie von Nucleinsäuren geeignet ist, welches einen pH im Bereich von etwa 4 bis 8 besitzt, umfassend
    a) ein Elutionssalz im Konzentrationsbereich von etwa 0,5 bis 1,5M, bestehend aus gleichen Konzentrationen von
    1) einem Kation gewählt aus Dialkylammonium, Trialkylammonium und Tetraalkylammonium, wobei die Alkylgruppen aus jeder Kombination von Methyl und Ethyl gewählt sind und
    2) einem Anion gewählt aus Bromid, Chlorid, Acetat, Format, Nitrat, Perchlorat, Dihydrogenphosphat, Ethansulfonat und Methansulfonat; und
    b) eine Puffersäure mit einem pKa im Bereich von etwa 3,5 bis 8,5, wobei die Säure in einer etwa 0,05M nicht übersteigenden Konzentration vorliegt, wobei ggf. die Puffersäure kationisch ist und eine Konjugatbase aufweist, die neutral oder basisch geladen ist; wobei die Absorption bei 260 nm (1 cm Strahlenweg) des Lösungsmittels relativ zu Wasser etwa 0,1 nicht übersteigt.
    2. Lösungsmittel nach Anspruch 1, welches ebenfalls eine Nucleinsäure im Konzentrationsbereich von etwa 1 M.g/1 bis 1 g/l umfaßt, wobei ggf. die Nucleinsäure doppelsträngige DNA umfaßt.
    3. Wäßrige Zusammensetzung umfassend etwa eine 2- bis 20-fache Konzentration eines Lösungsmittels nach Anspruch 1, so daß eine 1/2 bis 1/20 Verdünnung der Zusammensetzung in Wasser ein Lösungsmittel nach Anspruch 1 ergibt.
    4. Zusammensetzung umfassend die Kombination eines Lösungsmittels nach Anspruch 1 oder 2, mit einem Anionenaustauscherfeststoff, welcher Feststoff ggf. ein oder mehrere der folgenden Charakteristika aufweist:
    (a) der Feststoff weist ein Polyacryl- oder Polystyrolgerüst auf;
    0507531
    (b) der Feststoff umfaßt funktionelle Diethylaminoethylgruppen oder funktionelle Polyethylenimingruppen;
    (c) der Feststoff umfaßt Teilchen mit einem mittleren Durchmesser zwischen etwa 10 &mgr;&pgr;&igr; und 2 |lm; und/oder
    (d) der Feststoff ist im wesentlichen, ein nicht poröser.
    5. Verfahren zum Trennen und Analysieren von Nucleinsäuren auf der Basis von Unterschieden in der Molekülgröße, umfassend
    (a) das Kontaktieren einer Testprobe, welche Nucleinsäure enthält mit einem Anionenaustauscherfeststoff;
    (b) das Kontaktieren des Feststoffes von Schritt (a) mit einer Serie von Lösungsmitteln, welche wie in Anspruch 1 definiert sind, worin die Konzentration des Elutionssalzes systematisch von etwa 0,5M auf etwa 1,5M erhöht wird, so daß ein Hauptteil des Lösungsmittels von dem Feststoff nach dem Kontaktieren abgetrennt wird, und
    (c) Analysieren der Lösungsmittelserien in bezug auf Nucleinsäuren .
    6. Verfahren nach Anspruch 5, worin die Konzentration des Elutionssalzes, welches den Feststoff kontaktiert, kontinuierlich erhöht wird.
    7. Verfahren nach Anspruch 5 oder 6, worin die Konzentration der Nucleinsäure in dem von dem Feststoff abgetrennten Lösungsmittel durch Ultraviolettabsorption im Wellenlängenbereich von etwa 250 nm bis 2 90 nm gemessen wird.
    8. Verfahren nach einem der Ansprüche 5 bis 7, worin der Feststoff Teilchen mit einem mittleren Durchmesser zwischen etwa 10 &mgr;&pgr;&igr; und etwa 2 &mgr;&pgr;&igr; umfaßt.
    9. Verfahren nach einem der Ansprüche 5 bis 8, worin der Feststoff in einer zylindrischen Säule mit einem Durchmesser von etwa 1 mm bis 6 mm und einer Länge von etwa 10 mm und etwa 60 mm enthalten ist.
    10. Verfahren nach einem der Ansprüche 5 bis 9, worin die Gesamtzeit, um das Verfahren vollständig durchzuführen, zwischen etwa 2 min und etwa 30 min liegt.
    1992 11 02/Pa
DE199292302930T 1991-04-03 1992-04-03 Anionenaustauschtrennung von nukleinsaeuren. Pending DE507591T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US67973691A 1991-04-03 1991-04-03

Publications (1)

Publication Number Publication Date
DE507591T1 true DE507591T1 (de) 1993-03-18

Family

ID=24728140

Family Applications (3)

Application Number Title Priority Date Filing Date
DE69232677T Expired - Fee Related DE69232677T2 (de) 1991-04-03 1992-04-03 Verfahren zur Trennung doppelsträngiger DNA von einzelsträngiger DNA oder RNA
DE69220132T Expired - Fee Related DE69220132T2 (de) 1991-04-03 1992-04-03 Anionenaustauschtrennung von Nukleinsäuren
DE199292302930T Pending DE507591T1 (de) 1991-04-03 1992-04-03 Anionenaustauschtrennung von nukleinsaeuren.

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DE69232677T Expired - Fee Related DE69232677T2 (de) 1991-04-03 1992-04-03 Verfahren zur Trennung doppelsträngiger DNA von einzelsträngiger DNA oder RNA
DE69220132T Expired - Fee Related DE69220132T2 (de) 1991-04-03 1992-04-03 Anionenaustauschtrennung von Nukleinsäuren

Country Status (12)

Country Link
EP (2) EP0691148B1 (de)
JP (1) JP3195039B2 (de)
KR (1) KR920019931A (de)
AT (2) ATE220341T1 (de)
AU (1) AU645674B2 (de)
CA (1) CA2063855C (de)
DE (3) DE69232677T2 (de)
ES (1) ES2053416T1 (de)
GR (1) GR930300019T1 (de)
IE (1) IE921074A1 (de)
IL (1) IL101356A (de)
NZ (1) NZ242226A (de)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT398973B (de) * 1992-11-18 1995-02-27 Bonn Guenther Dr Verfahren zur trennung von nukleinsäuren
US5622822A (en) * 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5630924A (en) 1995-04-20 1997-05-20 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
US6355791B1 (en) 1995-11-13 2002-03-12 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography
US6066258A (en) * 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
KR100483498B1 (ko) 1996-02-14 2005-07-18 악조 노벨 엔.브이. 핵산물질의분리및증폭방법
SE9600796D0 (sv) * 1996-02-29 1996-02-29 Pharmacia Biotech Ab A method of preparing a liquid mixture
US6372142B1 (en) 1996-11-13 2002-04-16 Transgenomic, Inc. Column for DNA separation by matched ion polynucleotide chromatography
US6174441B1 (en) 1996-11-13 2001-01-16 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
US5972222A (en) * 1996-11-13 1999-10-26 Transgenomic, Inc. Process for performing polynucleotide separations
US6576133B2 (en) 1996-11-13 2003-06-10 Transgenomic, Inc Method and system for RNA analysis by matched ion polynucleotide chromatography
US7138518B1 (en) 1996-11-13 2006-11-21 Transgenomic, Inc. Liquid chromatographic separation of polynucleotides
US6471866B1 (en) 1996-11-13 2002-10-29 Transgenomic, Inc. Process for performing polynucleotide separations
US6287822B1 (en) 1997-08-05 2001-09-11 Transgenomic, Inc. Mutation detection method
US5997742A (en) * 1996-11-13 1999-12-07 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
US6017457A (en) * 1996-11-13 2000-01-25 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
US6482317B2 (en) 1996-11-13 2002-11-19 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US6475388B1 (en) 1996-11-13 2002-11-05 Transgenomic, Inc. Method and system for RNA analysis by matched ion polynucleotide chromatography
US6030527A (en) * 1996-11-13 2000-02-29 Transgenomic, Inc. Apparatus for performing polynucleotide separations using liquid chromatography
US6177559B1 (en) 1998-04-24 2001-01-23 Transgenomic, Inc. Process for separation of polynucleotide fragments
US5986085A (en) * 1997-04-25 1999-11-16 Transgenomic, Inc. Matched ion polynucleotide chromatography (MIPC) process for separation of polynucleotide fragments
US6056877A (en) * 1997-12-05 2000-05-02 Transgenomic, Inc. Non-polar media for polynucleotide separations
CA2288603A1 (en) 1997-06-10 1998-12-17 Robert M. Haefele System and method for performing polynucleotide separations using liquid chromatography
JP2002511767A (ja) * 1997-08-28 2002-04-16 ピーイー コーポレイション（エヌワイ） 化学切断による、核酸における変異の改良された検出
US6258264B1 (en) 1998-04-10 2001-07-10 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6372130B1 (en) 1997-12-05 2002-04-16 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6503397B2 (en) 1997-12-05 2003-01-07 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6265168B1 (en) 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides
US6485648B1 (en) 1998-05-18 2002-11-26 Transgenomic, Inc. MIPC column cleaning system and process
US6455692B1 (en) 1998-08-04 2002-09-24 Transgenomic, Inc. Method of concentrating polynucleotides using MIPC
US6074880A (en) 1998-08-28 2000-06-13 Transgenomic, Inc. Sample analyte containing solution fraction collection system, and method of use
AU1085601A (en) * 1999-10-12 2001-04-23 Transgenomic, Inc. Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
WO2001055713A2 (en) 2000-01-26 2001-08-02 Transgenomic, Inc. Method and apparatus for separating polynucleotides using monolithic capillary columns
US6969587B2 (en) 2000-04-04 2005-11-29 Taylor Paul D Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
KR100916949B1 (ko) * 2000-08-30 2009-09-14 에이브이아이 바이오파마 인코포레이티드 올리고뉴클레오티드 유사체의 분석 방법
US20030004371A1 (en) * 2001-05-23 2003-01-02 Gyula Vigh Cationic ion pairing compositions having volatile combustion products with O or S or halogen atoms or their combinations as heteroatoms for use with gas-phase or vapor-phase element-specific detectors
US20020197726A1 (en) * 2001-05-23 2002-12-26 Gyula Vigh Anionic ion pairing compositions having volatile combustion products with O or S or halogen atoms or their combinations as heteroatoms for use with gas-phase or vapor-phase element-specific detectors
WO2004011592A2 (en) * 2002-07-26 2004-02-05 Applera Corporation Petal-array support for use with microplates
DE102006020463B4 (de) * 2005-05-24 2013-05-23 Sartorius Stedim Biotech Gmbh Verfahren zum Entfernen von Nukleinsäuren aus einer Flüssigkeit, Verfahren zur Herstellung von Adsorberelementen und Verwendung von Membranadsorbermaterial-Verschnitt
AU2012205204B2 (en) * 2005-12-09 2015-03-12 Qiagen Gmbh Method for enriching short-chain nucleic acids
DE102005059315A1 (de) * 2005-12-09 2007-06-14 Qiagen Gmbh Verfahren zur Anreicherung von kurzkettigen Nukleinsäuren
WO2008045505A2 (en) 2006-10-10 2008-04-17 Xenomics, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
CN102388138A (zh) * 2009-02-11 2012-03-21 罗彻斯特大学 空间上异质官能化的多孔基质和用于选择性去除污染物的方法
JP5914338B2 (ja) * 2009-09-24 2016-05-11 キアジェン ゲイサーズバーグ インコーポレイテッド 陰イオン交換材料を使用した核酸の単離および分析のための組成物、方法およびキット
CN103348242B (zh) 2011-01-12 2016-08-17 积水医疗株式会社 单核苷酸多态性的检测方法
CN104946626A (zh) 2011-01-12 2015-09-30 积水医疗株式会社 离子交换色谱法用洗脱液以及核酸链的分析方法
EP2692863B1 (de) * 2011-03-31 2018-06-20 Sekisui Medical Co., Ltd. Pcr-primer zur herstellung von proben zur erkennung von einzelnukleotid-polymorphismen mittels allele-spezifischer pcr und ionenaustausch-chromatographie-analyse.
CN106380592B (zh) * 2016-11-04 2018-10-16 江南大学 一种从发酵液中提取ε-聚赖氨酸及其盐酸盐的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4672040A (en) * 1983-05-12 1987-06-09 Advanced Magnetics, Inc. Magnetic particles for use in separations
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
DE3717211A1 (de) * 1987-05-22 1988-12-01 Diagen Inst Molekularbio Vorrichtung und verfahren zur trennung und reinigung von molekuelen
US4959176A (en) * 1988-04-29 1990-09-25 Beckman Instruments, Inc. High resolution non corrosive amino acid analysis buffer solutions
SE8902315D0 (sv) * 1989-06-27 1989-06-27 Pharmacia Ab Anjonbytare
US5010183A (en) * 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents

Also Published As

Publication number Publication date
DE69220132T2 (de) 1997-11-13
DE69220132D1 (de) 1997-07-10
EP0691148B1 (de) 2002-07-10
DE69232677D1 (de) 2002-08-14
EP0691148A3 (de) 1996-01-24
JPH0599909A (ja) 1993-04-23
EP0691148A2 (de) 1996-01-10
CA2063855A1 (en) 1992-10-04
IL101356A0 (en) 1992-11-15
EP0507591A2 (de) 1992-10-07
EP0507591A3 (en) 1993-02-03
GR930300019T1 (en) 1993-04-28
ES2053416T1 (es) 1994-08-01
IE921074A1 (en) 1992-10-07
IL101356A (en) 1996-08-04
NZ242226A (en) 1993-07-27
AU645674B2 (en) 1994-01-20
DE69232677T2 (de) 2003-02-13
ATE154132T1 (de) 1997-06-15
JP3195039B2 (ja) 2001-08-06
EP0507591B1 (de) 1997-06-04
ATE220341T1 (de) 2002-07-15
CA2063855C (en) 1997-08-26
AU1403492A (en) 1992-10-08
KR920019931A (ko) 1992-11-20

Similar Documents

Publication Publication Date Title
DE507591T1 (de) Anionenaustauschtrennung von nukleinsaeuren.
DE60002952T2 (de) Verfahren zur selektiven Abtrennung und Reinigung von Eicosapentaensäure und Decosohexaensäure und deren Estern
DE2610698C2 (de)
EP0154246B1 (de) Phasenträger für die Verteilungschromatographie von Makromolekülen, Verfahren zu ihrer Herstellung und ihre Verwendung
DE69629403T2 (de) Verfahren zur herstellung von linkern zwischen markierungsgruppen und bio-organischen molekülen auf der basis von platin für die markierung von bio-organischen molekülen zur entdeckung interessanter biologischer substanzen
DE68904732T2 (de) Ionenaustausch und trennungsmethode.
DE2216719B2 (de) Verfahren zur Entfernung thermischer und radiolytischer Abbau- und Spaltprodukte aus einer Tributylphosphat enthaltenden Lösung
DE69822934T2 (de) Chirale Trennung von pharmazeutischen Verbindungen mit geladenen Cyclodextrinen durch Kapillarelektrophorese
DE3609021A1 (de) Chromatographisches trennmedium fuer die schnelle analyse von kleinen proben
DE3427923C2 (de)
DE69113617T2 (de) Unterdrückung der elektroendosmose bei der kapillarelektrophorese.
DE2505254C2 (de)
Hinze et al. Novel chiral separation techniques based on surfactants
DE3625266A1 (de) Mittel zur verwendung in einem zwei- oder mehrphasensystem
DE2625028C3 (de) Verfahren zur Herstellung von Komplexbildner in eingeschlossener Form enthaltenden Fäden
DE69007773T2 (de) Elektrophoretische Trennung in einem gelfreien Medium mittels gelösten Polymeren.
DE2029822C3 (de) Diagnostische Zusammensetzung zum Nachweis der Nikotinsäureproduktion durch Mycobakterien
DE60034676T2 (de) Verdrängungsreagenzien mit niedrigem molekulargewicht und hoher affinität zur reinigung von oligonukleotiden
DE60123030T2 (de) Stabilisierung von hochsensitiven nukleinsäurefärbungen in wässriger lösung
DE2350327C3 (de) Verfahren zum Reinigen von Polycarbonatlösungen
DE2031366A1 (de) Verwendung von Gelen zur Trennung von Nucleinsäurebausteinen
EP0352478B1 (de) Verfahren zur Trennung von in wässriger oder wässrig/organischer Lösung enthaltenen Substanzgemischen
DE3644346A1 (de) Matrixgebundene kronenetherliganden als trennmaterial in der affinitaetschromatographie von nukleinsaeuren
DE3110737C2 (de)
DE2818649A1 (de) Verfahren zur trennung von peptiden