DE69220132D1 - Anionenaustauschtrennung von Nukleinsäuren - Google Patents

Anionenaustauschtrennung von Nukleinsäuren

Info

Publication number
DE69220132D1
DE69220132D1 DE69220132T DE69220132T DE69220132D1 DE 69220132 D1 DE69220132 D1 DE 69220132D1 DE 69220132 T DE69220132 T DE 69220132T DE 69220132 T DE69220132 T DE 69220132T DE 69220132 D1 DE69220132 D1 DE 69220132D1
Authority
DE
Germany
Prior art keywords
nucleic acids
test sample
anion exchange
exchange separation
support material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69220132T
Other languages
English (en)
Other versions
DE69220132T2 (de
Inventor
Will Bloch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of DE69220132D1 publication Critical patent/DE69220132D1/de
Publication of DE69220132T2 publication Critical patent/DE69220132T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8804—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 automated systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8827—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
DE69220132T 1991-04-03 1992-04-03 Anionenaustauschtrennung von Nukleinsäuren Expired - Fee Related DE69220132T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US67973691A 1991-04-03 1991-04-03

Publications (2)

Publication Number Publication Date
DE69220132D1 true DE69220132D1 (de) 1997-07-10
DE69220132T2 DE69220132T2 (de) 1997-11-13

Family

ID=24728140

Family Applications (3)

Application Number Title Priority Date Filing Date
DE69220132T Expired - Fee Related DE69220132T2 (de) 1991-04-03 1992-04-03 Anionenaustauschtrennung von Nukleinsäuren
DE199292302930T Pending DE507591T1 (de) 1991-04-03 1992-04-03 Anionenaustauschtrennung von nukleinsaeuren.
DE69232677T Expired - Fee Related DE69232677T2 (de) 1991-04-03 1992-04-03 Verfahren zur Trennung doppelsträngiger DNA von einzelsträngiger DNA oder RNA

Family Applications After (2)

Application Number Title Priority Date Filing Date
DE199292302930T Pending DE507591T1 (de) 1991-04-03 1992-04-03 Anionenaustauschtrennung von nukleinsaeuren.
DE69232677T Expired - Fee Related DE69232677T2 (de) 1991-04-03 1992-04-03 Verfahren zur Trennung doppelsträngiger DNA von einzelsträngiger DNA oder RNA

Country Status (12)

Country Link
EP (2) EP0507591B1 (de)
JP (1) JP3195039B2 (de)
KR (1) KR920019931A (de)
AT (2) ATE154132T1 (de)
AU (1) AU645674B2 (de)
CA (1) CA2063855C (de)
DE (3) DE69220132T2 (de)
ES (1) ES2053416T1 (de)
GR (1) GR930300019T1 (de)
IE (1) IE921074A1 (de)
IL (1) IL101356A (de)
NZ (1) NZ242226A (de)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT398973B (de) * 1992-11-18 1995-02-27 Bonn Guenther Dr Verfahren zur trennung von nukleinsäuren
US5622822A (en) * 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5630924A (en) 1995-04-20 1997-05-20 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography
US6066258A (en) * 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US6355791B1 (en) 1995-11-13 2002-03-12 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
KR100483498B1 (ko) 1996-02-14 2005-07-18 악조 노벨 엔.브이. 핵산물질의분리및증폭방법
SE9600796D0 (sv) * 1996-02-29 1996-02-29 Pharmacia Biotech Ab A method of preparing a liquid mixture
US6287822B1 (en) 1997-08-05 2001-09-11 Transgenomic, Inc. Mutation detection method
US5997742A (en) * 1996-11-13 1999-12-07 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
US6372142B1 (en) 1996-11-13 2002-04-16 Transgenomic, Inc. Column for DNA separation by matched ion polynucleotide chromatography
US6471866B1 (en) 1996-11-13 2002-10-29 Transgenomic, Inc. Process for performing polynucleotide separations
US6174441B1 (en) 1996-11-13 2001-01-16 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
US7138518B1 (en) 1996-11-13 2006-11-21 Transgenomic, Inc. Liquid chromatographic separation of polynucleotides
US5972222A (en) * 1996-11-13 1999-10-26 Transgenomic, Inc. Process for performing polynucleotide separations
US6017457A (en) * 1996-11-13 2000-01-25 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
US6482317B2 (en) 1996-11-13 2002-11-19 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US6030527A (en) * 1996-11-13 2000-02-29 Transgenomic, Inc. Apparatus for performing polynucleotide separations using liquid chromatography
US6576133B2 (en) 1996-11-13 2003-06-10 Transgenomic, Inc Method and system for RNA analysis by matched ion polynucleotide chromatography
US6475388B1 (en) 1996-11-13 2002-11-05 Transgenomic, Inc. Method and system for RNA analysis by matched ion polynucleotide chromatography
US6177559B1 (en) 1998-04-24 2001-01-23 Transgenomic, Inc. Process for separation of polynucleotide fragments
US5986085A (en) * 1997-04-25 1999-11-16 Transgenomic, Inc. Matched ion polynucleotide chromatography (MIPC) process for separation of polynucleotide fragments
US6056877A (en) * 1997-12-05 2000-05-02 Transgenomic, Inc. Non-polar media for polynucleotide separations
DE69817207T2 (de) 1997-06-10 2004-06-17 Transgenomic, Inc., San Jose System und verfahren zur durchführung von polynukleotid-trennungen mit hilfe von flüssigchromatographie
JP2002511767A (ja) * 1997-08-28 2002-04-16 ピーイー コーポレイション（エヌワイ） 化学切断による、核酸における変異の改良された検出
US6503397B2 (en) 1997-12-05 2003-01-07 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6258264B1 (en) 1998-04-10 2001-07-10 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6372130B1 (en) 1997-12-05 2002-04-16 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6265168B1 (en) 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides
US6485648B1 (en) 1998-05-18 2002-11-26 Transgenomic, Inc. MIPC column cleaning system and process
US6455692B1 (en) 1998-08-04 2002-09-24 Transgenomic, Inc. Method of concentrating polynucleotides using MIPC
US6074880A (en) 1998-08-28 2000-06-13 Transgenomic, Inc. Sample analyte containing solution fraction collection system, and method of use
JP2004514874A (ja) * 1999-10-12 2004-05-20 トランスジエノミツク・インコーポレーテツド アニオン−交換クロマトグラフィーによる核酸ヘテロ二本鎖分子の検出
EP1235852B1 (de) 2000-01-26 2011-06-15 TRANSGENOMIC, Inc. Verfahren zur trennung von polynukleotidionen mittels monolithischer kapillarsäulen
US6969587B2 (en) 2000-04-04 2005-11-29 Taylor Paul D Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
CA2421043C (en) * 2000-08-30 2012-07-31 Avi Biopharma, Inc. Method for analysis of oligonucleotide analogs
US20030004371A1 (en) * 2001-05-23 2003-01-02 Gyula Vigh Cationic ion pairing compositions having volatile combustion products with O or S or halogen atoms or their combinations as heteroatoms for use with gas-phase or vapor-phase element-specific detectors
US20020197726A1 (en) * 2001-05-23 2002-12-26 Gyula Vigh Anionic ion pairing compositions having volatile combustion products with O or S or halogen atoms or their combinations as heteroatoms for use with gas-phase or vapor-phase element-specific detectors
AU2003254175A1 (en) * 2002-07-26 2004-02-16 Applera Corporation Device and method for purification of nucleic acids
DE102006020463B4 (de) * 2005-05-24 2013-05-23 Sartorius Stedim Biotech Gmbh Verfahren zum Entfernen von Nukleinsäuren aus einer Flüssigkeit, Verfahren zur Herstellung von Adsorberelementen und Verwendung von Membranadsorbermaterial-Verschnitt
DE102005059315A1 (de) * 2005-12-09 2007-06-14 Qiagen Gmbh Verfahren zur Anreicherung von kurzkettigen Nukleinsäuren
AU2012205204B2 (en) * 2005-12-09 2015-03-12 Qiagen Gmbh Method for enriching short-chain nucleic acids
US9163229B2 (en) 2006-10-10 2015-10-20 Trovagene, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
EP2396405B1 (de) * 2009-02-11 2016-12-14 University Of Rochester Räumlich inhomogen funktionalisierte poröse medien und verfahren für ihre verwendung zur selektiven schadstoffentfernung
JP5914338B2 (ja) * 2009-09-24 2016-05-11 キアジェン ゲイサーズバーグ インコーポレイテッド 陰イオン交換材料を使用した核酸の単離および分析のための組成物、方法およびキット
CN103348242B (zh) 2011-01-12 2016-08-17 积水医疗株式会社 单核苷酸多态性的检测方法
US9481881B2 (en) 2011-01-12 2016-11-01 Sekisui Medical Co., Ltd. Eluent for ion-exchange chromatography, and method of analyzing nucleic acid chains
KR20140026426A (ko) * 2011-03-31 2014-03-05 세키스이 메디칼 가부시키가이샤 단일 염기 다형 검출용의 시료 핵산, 단일 염기 다형 검출 시료 조제용의 ｐｃｒ 프라이머 및 이온 교환 크로마토그래피 분석에 사용하는 단일 염기 다형 검출용 시료의 조제 방법
CN106380592B (zh) * 2016-11-04 2018-10-16 江南大学 一种从发酵液中提取ε-聚赖氨酸及其盐酸盐的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4672040A (en) * 1983-05-12 1987-06-09 Advanced Magnetics, Inc. Magnetic particles for use in separations
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
DE3717211A1 (de) * 1987-05-22 1988-12-01 Diagen Inst Molekularbio Vorrichtung und verfahren zur trennung und reinigung von molekuelen
US4959176A (en) * 1988-04-29 1990-09-25 Beckman Instruments, Inc. High resolution non corrosive amino acid analysis buffer solutions
SE8902315D0 (sv) * 1989-06-27 1989-06-27 Pharmacia Ab Anjonbytare
US5010183A (en) * 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents

Also Published As

Publication number Publication date
DE69232677D1 (de) 2002-08-14
KR920019931A (ko) 1992-11-20
GR930300019T1 (en) 1993-04-28
EP0507591A3 (en) 1993-02-03
EP0691148B1 (de) 2002-07-10
EP0507591B1 (de) 1997-06-04
JP3195039B2 (ja) 2001-08-06
DE507591T1 (de) 1993-03-18
IL101356A0 (en) 1992-11-15
AU645674B2 (en) 1994-01-20
DE69232677T2 (de) 2003-02-13
JPH0599909A (ja) 1993-04-23
IE921074A1 (en) 1992-10-07
NZ242226A (en) 1993-07-27
ES2053416T1 (es) 1994-08-01
EP0691148A3 (de) 1996-01-24
DE69220132T2 (de) 1997-11-13
ATE220341T1 (de) 2002-07-15
CA2063855C (en) 1997-08-26
EP0507591A2 (de) 1992-10-07
AU1403492A (en) 1992-10-08
EP0691148A2 (de) 1996-01-10
ATE154132T1 (de) 1997-06-15
IL101356A (en) 1996-08-04
CA2063855A1 (en) 1992-10-04

Similar Documents

Publication Publication Date Title
DE69220132D1 (de) Anionenaustauschtrennung von Nukleinsäuren
EP1961826A3 (de) Verfahren zur direkten Sequenzierung von Nukleinsäuren
DE50015331D1 (de) Verfahren zum anbinden von nukleinsäuren an eine festphase
NO881064L (no) Fremgangsmaate til bestemmelse av nukleinsyrer ved hybridiseringsteknikk, reagenskombinasjon og testpakke til utfoerelse av fremgangmaaten.
DE69533317D1 (de) Isolierung von nukleinsäuren
ES2143680T3 (es) Metodo para caracterizar dna genomico.
ATE66695T1 (de) Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten.
CA2262920A1 (en) Method for identifying compounds affecting rna/rna binding protein interactions
DE69305662D1 (de) Brett-stabiles Produkt und Verfahren zur Isolierung der RNA, DNA und Proteine
RU92000528A (ru) Способ размножения, экспрессии, диагностики и клонирования нуклеиновых кислот в иммобилизованных средах
IT1155089B (it) Frammenti di adn contrassegnati in corrispondenza di almeno una delle loro estremita' tramite ribonucleotidi modificati riconoscibili medinate molecole affini e procedimento per realizzare una analisi di tali frammenti
DE69017826D1 (de) Membranverfahren zum Trennen von organischen Flüssigkeiten.
DE3689412D1 (de) Verfahren zur durchführung von hybridisierungstests von nukleinsäuren.
BR8904560A (pt) Membrana de afinidade polimerica,processo para preparacao de membranas de afinidade e processo para seletivamente remover materiais biologicos de fluidos
DK242685A (da) Fremgangsmaade og reagenssystem til bestemmelse af en bestemt polynucleotidsekvens i et testmedium indeholdende enkeltstrengede nucleinsyrer
DE60029794D1 (de) Verwendung von mit polymer beschichtetem sorptionsmittelkomposit zur trennung, reinigung, entsalzung und konzentrierung von biopolymeren
DE3850468D1 (de) System zum Detektierung von ONS.
IL71519A0 (en) Terminally labeled dna molecules or probes and methods and reagent kits for the preparation thereof
FI970832A (fi) Biologisen materiaalin, kuten nukleiinihapon, erottamiseen käytettävä laite
DE3689398D1 (de) Verfahren zum Extrahieren von Nukleinsäuren aus Zellen.
WO2006025074A3 (en) Twodimensional strandness- and length-dependent separation of nucleic acid fragments
DE68919387D1 (de) DNS- und RNS-Proben, Methoden und Testsätze zum Nachweis von Salmonella.
WO1999027131A3 (de) Topologisch fixiertes, matrixgebundenes nukleinsäuremolekül
ES2072864T3 (es) Procedimiento rapido para la identificacion de una secuencia especifica de acidos nucleicos.
IL73578A (en) Method and reagent for the detection of polynucleotide sequence in sample of dna or rna by using a nucleic acid probe and contact of duplexes with immobilizing antibody

Legal Events

Date Code Title Description
8328 Change in the person/name/address of the agent

Free format text: DERZEIT KEIN VERTRETER BESTELLT

8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: APPLERA CORP. (N.D.GES.D. STAATES DELAWARE), FOSTE

8339 Ceased/non-payment of the annual fee