DE3689846D1 - Erhöhte ausscheidung von heterologen proteinen durch wirtszellen durch verwendung von substituierten promotoren. - Google Patents
Erhöhte ausscheidung von heterologen proteinen durch wirtszellen durch verwendung von substituierten promotoren.Info
- Publication number
- DE3689846D1 DE3689846D1 DE3689846T DE3689846T DE3689846D1 DE 3689846 D1 DE3689846 D1 DE 3689846D1 DE 3689846 T DE3689846 T DE 3689846T DE 3689846 T DE3689846 T DE 3689846T DE 3689846 D1 DE3689846 D1 DE 3689846D1
- Authority
- DE
- Germany
- Prior art keywords
- dna
- promotors
- substituted
- host cells
- signal sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 3
- 102000007469 Actins Human genes 0.000 abstract 2
- 108010085238 Actins Proteins 0.000 abstract 2
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 abstract 2
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 abstract 2
- 102000003978 Tissue Plasminogen Activator Human genes 0.000 abstract 2
- 108090000373 Tissue Plasminogen Activator Proteins 0.000 abstract 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 abstract 2
- 230000028327 secretion Effects 0.000 abstract 2
- 229960000187 tissue plasminogen activator Drugs 0.000 abstract 2
- 102000003390 tumor necrosis factor Human genes 0.000 abstract 2
- 102100028991 Cytochrome c1, heme protein, mitochondrial Human genes 0.000 abstract 1
- 101000916041 Homo sapiens Cytochrome c1, heme protein, mitochondrial Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 101000947508 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Cytochrome c isoform 1 Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Diabetes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Lubricants (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB858529014A GB8529014D0 (en) | 1985-11-25 | 1985-11-25 | Enhanced secretion of heterologous proteins |
PCT/EP1986/000675 WO1987003300A1 (en) | 1985-11-25 | 1986-11-24 | Enhanced secretion of heterologous proteins by hosts using substituted promoters |
Publications (2)
Publication Number | Publication Date |
---|---|
DE3689846D1 true DE3689846D1 (de) | 1994-06-23 |
DE3689846T2 DE3689846T2 (de) | 1994-09-22 |
Family
ID=10588758
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE3689846T Expired - Lifetime DE3689846T2 (de) | 1985-11-25 | 1986-11-24 | Erhöhte ausscheidung von heterologen proteinen durch wirtszellen durch verwendung von substituierten promotoren. |
Country Status (8)
Country | Link |
---|---|
US (1) | US5082783A (de) |
EP (1) | EP0283475B1 (de) |
JP (2) | JP2528849B2 (de) |
AT (1) | ATE105865T1 (de) |
CA (1) | CA1318617C (de) |
DE (1) | DE3689846T2 (de) |
GB (1) | GB8529014D0 (de) |
WO (1) | WO1987003300A1 (de) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0281246A3 (de) * | 1987-02-04 | 1989-07-12 | Invitron Corporation | Verfahren zur Erhöhung der Amplifikation und Expression fremder Gene |
US5030563A (en) * | 1987-07-07 | 1991-07-09 | Genetics Institute, Inc. | Bacterial hypersecretion using mutant repressor sequence |
DE68927933T2 (de) * | 1988-09-02 | 1997-08-14 | Dyax Corp | Herstellung und auswahl von rekombinantproteinen mit verschiedenen bindestellen |
US5326700A (en) * | 1990-11-06 | 1994-07-05 | Eli Lilly And Company | DNA sequences encoding t-PA derivatives with cleavable sites |
DE4107612A1 (de) * | 1991-03-09 | 1992-09-10 | Behringwerke Ag | Rekombinante proteine mit der immunreaktivitaet des hepatitis b virus e antigens (hbeag), verfahren zu ihrer herstellung und ihre verwendung in immunoassays und impfstoffen |
US5925564A (en) * | 1991-11-06 | 1999-07-20 | Baylor College Of Medicine | Expression vector systems and method of use |
US5298422A (en) * | 1991-11-06 | 1994-03-29 | Baylor College Of Medicine | Myogenic vector systems |
WO1995016772A1 (en) | 1993-12-14 | 1995-06-22 | Cornell Research Foundation, Inc. | Adenovirus gene expression system |
JPH11507205A (ja) | 1995-04-27 | 1999-06-29 | ヒューマン・ジェノム・サイエンシズ・インコーポレイテッド | ヒト腫瘍壊死因子受容体 |
US5925351A (en) | 1995-07-21 | 1999-07-20 | Biogen, Inc. | Soluble lymphotoxin-β receptors and anti-lymphotoxin receptor and ligand antibodies as therapeutic agents for the treatment of immunological disease |
EP0878552A1 (de) * | 1997-05-13 | 1998-11-18 | Erasmus Universiteit Rotterdam | Molekularer Nachweis von chromosomalen Veränderungen |
EP1078098A1 (de) * | 1998-05-04 | 2001-02-28 | Dako A/S | Verfahren und sonden zur detektion chromosomaler aberrationen |
AUPP655698A0 (en) * | 1998-10-16 | 1998-11-05 | Commonwealth Scientific And Industrial Research Organisation | Delivery system for porcine somatotropin |
US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
GB0314856D0 (en) * | 2003-06-25 | 2003-07-30 | Unitargeting Res As | Protein expression system |
WO2005000898A2 (en) * | 2003-06-27 | 2005-01-06 | Biogen Idec Ma Inc. | Use of hydrophobic-interaction-chromatography or hinge-region modifications for the production of homogeneous antibody-solutions |
EP2327718B1 (de) * | 2003-11-21 | 2016-03-23 | Pfenex, Inc. | Verbessertes Expressionssystem mit SEC-System-Sekretion |
EP1756162A1 (de) * | 2004-03-23 | 2007-02-28 | Biogen Idec MA Inc. | Rezeptor-kupplungsmittel und ihre therapeutischen verwendungen |
JP2008507294A (ja) | 2004-07-26 | 2008-03-13 | ダウ グローバル テクノロジーズ インコーポレイティド | 菌株遺伝子操作による改善されたタンパク質発現のための方法 |
WO2006074399A2 (en) * | 2005-01-05 | 2006-07-13 | Biogen Idec Ma Inc. | Multispecific binding molecules comprising connecting peptides |
MX2009004134A (es) * | 2006-10-20 | 2009-08-12 | Biogen Idec Inc | Tratamiento de trastornos de desmielinizacion con el receptor de linfotoxina beta soluble. |
US8338376B2 (en) * | 2006-10-20 | 2012-12-25 | Biogen Idec Ma Inc. | Compositions comprising variant LT-B-R-IG fusion proteins |
MX2009004718A (es) | 2006-11-02 | 2009-06-19 | Acceleron Pharma Inc | Antagonistas de receptor de alk1 y ligando y sus usos. |
US8158584B2 (en) | 2008-05-02 | 2012-04-17 | Acceleron Pharma, Inc. | Pharmaceutical preparations comprising an ALK1-Fc fusion protein |
KR101491867B1 (ko) | 2007-01-31 | 2015-02-10 | 피페넥스 인크. | 증가된 발현을 위한 박테리아 리더 서열 |
JP5444553B2 (ja) | 2007-04-27 | 2014-03-19 | フェネックス インコーポレイテッド | 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および/または質が改善されている特定の株を同定する方法 |
US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
WO2009124012A1 (en) * | 2008-03-31 | 2009-10-08 | Dow Global Technologies Inc. | A design for rapidly cloning one or more polypeptide chains into an expression system |
JP2010183885A (ja) * | 2009-02-13 | 2010-08-26 | Kobe Univ | タンパク質の製造方法およびそれに用いる発現ベクター |
CN108178789B (zh) | 2011-04-20 | 2021-11-02 | 阿塞勒隆制药公司 | 内皮糖蛋白多肽及其用途 |
EP2809335A4 (de) | 2012-02-02 | 2015-10-28 | Acceleron Pharma Inc | Alk1-antagonisten und deren verwendung zur behandlung von nierenzellenkarzinomen |
AU2014248768A1 (en) | 2013-03-12 | 2015-09-24 | Massachusetts Eye And Ear Infirmary | Modified mullerian inhibiting substance (MIS) proteins and uses thereof for the treatment of diseases |
EP3705498A1 (de) | 2013-08-22 | 2020-09-09 | Acceleron Pharma Inc. | Varianten von tgf-beta-rezeptortyp ii und verwendungen davon |
CA2928708A1 (en) | 2013-10-25 | 2015-04-30 | Acceleron Pharma, Inc. | Endoglin peptides to treat fibrotic diseases |
CA3151082A1 (en) | 2013-12-11 | 2015-06-18 | The General Hospital Corporation | Use of mullerian inhibiting substance (mis) proteins for contraception and ovarian reserve preservation |
US9884900B2 (en) | 2015-08-04 | 2018-02-06 | Acceleron Pharma Inc. | Methods for treating Janus kinase-associated disorders by administering soluble transforming growth factor beta type II receptor |
DK3628049T3 (da) | 2017-05-04 | 2023-08-14 | Acceleron Pharma Inc | Tgf-beta-receptortype-ii-fusionsproteiner og anvendelser deraf |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4411994A (en) * | 1978-06-08 | 1983-10-25 | The President And Fellows Of Harvard College | Protein synthesis |
US4338397A (en) * | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
US4546082A (en) * | 1982-06-17 | 1985-10-08 | Regents Of The Univ. Of California | E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins |
DE3382547D1 (de) * | 1983-01-12 | 1992-05-27 | Chiron Corp | Sekretorische expression in eukaryoten. |
DE3486216T2 (de) * | 1983-04-25 | 1994-02-17 | Chiron Corp | Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren. |
US4588684A (en) * | 1983-04-26 | 1986-05-13 | Chiron Corporation | a-Factor and its processing signals |
IL71991A (en) * | 1983-06-06 | 1994-05-30 | Genentech Inc | Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes |
FR2579224B1 (fr) * | 1985-03-25 | 1987-05-22 | Genetica | Procede de preparation microbiologique de la serum-albumine humaine |
-
1985
- 1985-11-25 GB GB858529014A patent/GB8529014D0/en active Pending
-
1986
- 1986-11-24 WO PCT/EP1986/000675 patent/WO1987003300A1/en active IP Right Grant
- 1986-11-24 EP EP86906826A patent/EP0283475B1/de not_active Expired - Lifetime
- 1986-11-24 AT AT86906826T patent/ATE105865T1/de not_active IP Right Cessation
- 1986-11-24 DE DE3689846T patent/DE3689846T2/de not_active Expired - Lifetime
- 1986-11-24 JP JP61506239A patent/JP2528849B2/ja not_active Expired - Lifetime
- 1986-11-24 CA CA000523697A patent/CA1318617C/en not_active Expired - Lifetime
-
1989
- 1989-12-18 US US07/452,632 patent/US5082783A/en not_active Expired - Lifetime
-
1994
- 1994-12-07 JP JP6303910A patent/JP2572952B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JPH07222595A (ja) | 1995-08-22 |
JP2528849B2 (ja) | 1996-08-28 |
CA1318617C (en) | 1993-06-01 |
EP0283475A1 (de) | 1988-09-28 |
EP0283475B1 (de) | 1994-05-18 |
DE3689846T2 (de) | 1994-09-22 |
US5082783A (en) | 1992-01-21 |
GB8529014D0 (en) | 1986-01-02 |
WO1987003300A1 (en) | 1987-06-04 |
ATE105865T1 (de) | 1994-06-15 |
JPS63502717A (ja) | 1988-10-13 |
JP2572952B2 (ja) | 1997-01-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE3689846T2 (de) | Erhöhte ausscheidung von heterologen proteinen durch wirtszellen durch verwendung von substituierten promotoren. | |
ATE90966T1 (de) | Expression von protein-c. | |
KR860008285A (ko) | 심방의 나트륨 이뇨성/혈관 확장성 폴리펩티드의 제조방법 | |
ES551913A0 (es) | Un metodo para obtener expresion de una secuencia de codificacion deseada en celulas de mamifero | |
IE811463L (en) | Interferons | |
CA2032153A1 (fr) | Application de nouveaux fragments d'adn en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant | |
ATE46536T1 (de) | Verfahren zur herstellung von reifen proteinen in wirtzellen von wirbeltieren. | |
AU6801487A (en) | Method of producing a recombinant protein complex having human factor VIII:C activity | |
DE3688900T3 (de) | Expression von Protein C. | |
FI922204A0 (fi) | Proteinkomplex med faktor viii:c- aktivitet och framstaellning av desamma. | |
ES8706208A1 (es) | Un metodo para preparar proteinas que son inmunologicamente reactivas con anticuerpos para virus asociados con linfadenopatia | |
ES2078243T3 (es) | Mejoras en o relativas a la expresion eucariotica. | |
ATE247164T1 (de) | Insulinartigen wachstumsfaktor bindendes protein | |
KR870006185A (ko) | 트롬빈 억제제의 제조방법 | |
EP0165759A3 (de) | Säugetierpromotoren verwendbar in Hefeexpression | |
RU92004585A (ru) | Промотор, днк-последовательность, вектор, клетки, способ экспрессии днк | |
RU93004936A (ru) | Днк, штаммы, способы получения днк, способы получения полипептидов | |
RU95101019A (ru) | Способ получения фактора некроза опухоли, фактор некроза опухоли, днк, клетка, композиция | |
JPS642586A (en) | Western subtype dna and rna molecule of fsme-virus, polypeptide coded by the molecule and its usage | |
FI934079A (fi) | Menetelmä fuusioproteiinin valmistamiseksi |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: BIOGEN IDEC MA INC., CAMBRIDGE, MASS., US |