ES2078243T3 - Mejoras en o relativas a la expresion eucariotica. - Google Patents

Mejoras en o relativas a la expresion eucariotica.

Info

Publication number
ES2078243T3
ES2078243T3 ES89310062T ES89310062T ES2078243T3 ES 2078243 T3 ES2078243 T3 ES 2078243T3 ES 89310062 T ES89310062 T ES 89310062T ES 89310062 T ES89310062 T ES 89310062T ES 2078243 T3 ES2078243 T3 ES 2078243T3
Authority
ES
Spain
Prior art keywords
expression
cell
promoter
dna sequence
useful substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89310062T
Other languages
English (en)
Inventor
David Thompson Berg
Brian William Grinnell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of ES2078243T3 publication Critical patent/ES2078243T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/30—Vector systems having a special element relevant for transcription being an enhancer not forming part of the promoter region
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/55—Vector systems having a special element relevant for transcription from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

SE SUMINISTRA UN METODO PARA PRODUCIR UNA SUSTANCIA UTIL EN CELULAS ANFITRIONAS EUKARIOTICAS EN EL CUAL LA CELULA SE TRANSFORMA CON UN VECTOR DE DNA RECOMBINANTE QUE COMPRENDE UN PROMOTOR EUKARIOTICO, UN ELEMENTO POLI - GT POSICIONADO PARA ESTIMULAR EL PROMOTOR, Y UNA SECUENCIA DE DNA QUE CODIFICA LA SUSTANCIA UTIL. LA SECUENCIA DE DNA ESTA POSICIONA PARA SU EXPRESION A PARTIR DEL PROMOTOR, Y EN EL CUAL LA CELULA QUE CONTIENE EL VECTOR SE CULTIVA HBAJO CONDICIONES ADECUADAS PARA LA EXPRESION DE LA SUSTANCIA UTIL, QUE COMPRENDE: A) EL APROVISIONAMIENTO DE LA CELULA CON UNA SECUENCIA DE DNA QUE CODIFICA LA EXPRESION DE UN PRODUCTO DE GEN INMEDIATAMENTE ANTERIOR DE UN VIRUS DE DNA LARGO, B) EL CULTIVO DE LA CELULA DEL PASO A BAJO CONDICIONES ADECUADAS PARA LA EXPRESION DEL CULTIVO DE GEN Y LA ESTIMULACION DE LA ACTIVIDAD DEL ELEMENTO POLI - GT.
ES89310062T 1988-10-07 1989-10-02 Mejoras en o relativas a la expresion eucariotica. Expired - Lifetime ES2078243T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US25520388A 1988-10-07 1988-10-07

Publications (1)

Publication Number Publication Date
ES2078243T3 true ES2078243T3 (es) 1995-12-16

Family

ID=22967292

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89310062T Expired - Lifetime ES2078243T3 (es) 1988-10-07 1989-10-02 Mejoras en o relativas a la expresion eucariotica.

Country Status (10)

Country Link
US (1) US5506118A (es)
EP (1) EP0363127B1 (es)
JP (1) JP3004660B2 (es)
AT (1) ATE126275T1 (es)
CA (1) CA1332049C (es)
DE (1) DE68923753T2 (es)
DK (1) DK488289A (es)
ES (1) ES2078243T3 (es)
GR (1) GR3017101T3 (es)
IL (1) IL91888A (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5550036A (en) * 1986-04-09 1996-08-27 Eli Lilly And Company Method for co-amplification of human protein C genes in human cells
US5270178A (en) * 1990-02-23 1993-12-14 Eli Lilly And Company Vectors and compounds for expression of zymogen forms of human protein C
IL97312A (en) 1990-02-23 1999-01-26 Lilly Co Eli A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
US5242688A (en) * 1990-12-24 1993-09-07 Eli Lilly And Company Method of treating thromboembolic disorders by administration of diglycosylated t-pa variants
US5733720A (en) * 1992-06-18 1998-03-31 Washington University Genetically engineered cell lines for detecting infectious herpesvirus and methods therefor
US5958676A (en) * 1992-06-18 1999-09-28 Washington University Genetically engineered cell lines for detecting infectious herpesvirus and methods therefor
US5618714A (en) * 1993-12-15 1997-04-08 Eli Lilly And Company Methods for producing protein C
DE4410188A1 (de) * 1994-03-24 1995-09-28 Boehringer Mannheim Gmbh Gentherapeutisches Verfahren unter Verwendung von antibiotikaresistenzgenfreien DNA-Vektoren
ATE171728T1 (de) * 1994-07-29 1998-10-15 Teijin Ltd Trypsintyp-enzyme, dafür kodierende nukleinsäuresequenz und verfahren zur herstellung des enzymes
AU2003206251B2 (en) * 2002-01-09 2008-03-13 Crucell Holland B.V. Use of erythropoietin for the preventive or curative treatment of cardiac failure
KR20080028888A (ko) 2005-06-24 2008-04-02 데이진 화-마 가부시키가이샤 신규 생리 물질 ｎｅｓｆａｔｉｎ과 그 관련 물질, 및그들의 용도

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4562155A (en) * 1983-05-19 1985-12-31 The Wistar Institute Of Anatomy And Biology Hybrid viral plasmid and microorganisms containing same
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
JPH0789930B2 (ja) * 1984-03-08 1995-10-04 大正製薬株式会社 ウイルス性転写促進配列
US4775624A (en) * 1985-02-08 1988-10-04 Eli Lilly And Company Vectors and compounds for expression of human protein C
ATE93272T1 (de) * 1985-06-27 1993-09-15 Zymogenetics Inc Expression von protein c.
EP0245949B1 (en) * 1986-04-09 1997-10-29 Eli Lilly And Company A method of using eukaryotic expression vectors comprising the bk virus enhancer
US4775630A (en) * 1986-08-15 1988-10-04 Vanderbilt University Transcriptional control element adapted for regulation of gene expression in animal cells

Also Published As

Publication number Publication date
ATE126275T1 (de) 1995-08-15
DE68923753D1 (de) 1995-09-14
DE68923753T2 (de) 1996-02-15
GR3017101T3 (en) 1995-11-30
EP0363127A3 (en) 1992-03-04
US5506118A (en) 1996-04-09
CA1332049C (en) 1994-09-20
JPH02171198A (ja) 1990-07-02
EP0363127A2 (en) 1990-04-11
DK488289A (da) 1990-04-08
DK488289D0 (da) 1989-10-04
IL91888A0 (en) 1990-06-10
EP0363127B1 (en) 1995-08-09
IL91888A (en) 1995-12-31
JP3004660B2 (ja) 2000-01-31

Similar Documents

Publication Publication Date Title
DE3786321D1 (de) Expression von protein-c.
ATE153068T1 (de) Interleukin-4-rezeptoren
ES2013324A6 (es) Un metodo mejorado para producir una proteina heterologa.
IL89011A0 (en) Receptor protein,dna sequence coding therefor and process for the preparation thereof
ES2153444T3 (es) Anticuerpos contra interleucina-4 mamifera, y peptidos utiles como antigenos en su produccion.
ES2122955T3 (es) Adn que codifica proteinas que se unen a la il-1 humana.
ATE93272T1 (de) Expression von protein c.
DE69636878D1 (de) Zirkuläres DNA Molekül mit einem abhängigen Replikationsursprung, seine Herstellungsverfahren und seine Verwendung in der Gentherapie
ES2078243T3 (es) Mejoras en o relativas a la expresion eucariotica.
GB8718779D0 (en) Dna sequence & expression vector
DE3851035D1 (de) Interleukin-7.
GR3015689T3 (en) Process for the construction of an animal cell line for the production of human beta-interferon.
ATE243741T1 (de) Zufuhr von genprodukten mittels mesangium-zellen
EP0282330A3 (en) Method for stimulating gene expression
HUP9800520A2 (hu) Génexpresszió emlős sejtekben
ES8705921A1 (es) Un procedimiento para mejorar la produccion de un polipeptido deseado en un hospedante transformado con una molecula de adn recombinantes.
DE58909205D1 (de) Expressionsvektor und Verfahren zur regulierbaren Herstellung von Proteinen in Eukaryonten.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 363127

Country of ref document: ES