CN115156215A - Magnetic particle cleaning device and cleaning method for immunoassay device - Google Patents
Magnetic particle cleaning device and cleaning method for immunoassay device Download PDFInfo
- Publication number
- CN115156215A CN115156215A CN202210922251.XA CN202210922251A CN115156215A CN 115156215 A CN115156215 A CN 115156215A CN 202210922251 A CN202210922251 A CN 202210922251A CN 115156215 A CN115156215 A CN 115156215A
- Authority
- CN
- China
- Prior art keywords
- reaction vessel
- wall
- magnetic block
- cleaning
- magnetic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004140 cleaning Methods 0.000 title claims abstract description 218
- 239000006249 magnetic particle Substances 0.000 title claims abstract description 137
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000003018 immunoassay Methods 0.000 title claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 90
- 239000002699 waste material Substances 0.000 claims abstract description 73
- 238000002347 injection Methods 0.000 claims abstract description 37
- 239000007924 injection Substances 0.000 claims abstract description 37
- 238000000605 extraction Methods 0.000 claims description 37
- 238000005406 washing Methods 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 14
- 238000004458 analytical method Methods 0.000 claims description 3
- 230000001788 irregular Effects 0.000 claims description 3
- 230000001900 immune effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 4
- 238000005086 pumping Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 8
- 239000012491 analyte Substances 0.000 description 3
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/093—Cleaning containers, e.g. tanks by the force of jets or sprays
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention aims to provide a magnetic particle cleaning device and a cleaning method for immunoassay equipment, which comprise an initial station, a first cleaning station, a second cleaning station, a third cleaning station and a fourth cleaning station which are arranged in sequence; a first magnetic block is arranged on the side edge of the first cleaning station, and a first waste extracting needle is arranged at the top of the first cleaning station; a first cleaning liquid injection needle is arranged at the top of the second cleaning station; a second magnetic block is arranged on the side edge of the third cleaning station and is close to the second outer wall of the reaction container; the side of fourth cleaning station sets up the third magnetic path, the top of fourth cleaning station sets up the second and takes out useless needle, and the technological effect is as follows: the cleaning device is used for pumping waste liquid and filling cleaning liquid through multi-station matching, and a liquid outlet of a first cleaning liquid injection needle is aligned to a first inner wall of the reaction container corresponding to the first outer wall, so that the cleaning liquid is directly aligned to magnetic particles attached to the first inner wall after combination, and the cleaning is more thorough.
Description
Technical Field
The invention relates to the technical field of immunoassay equipment, in particular to a magnetic particle cleaning device and a cleaning method for immunoassay equipment.
Background
The immunoassay device is used for detection and analysis techniques of analytes such as various antigens, haptens, antibodies, hormones and the like. The magnetic particle chemiluminescence immune analysis is a labeling immune determination technology for detecting trace substance to be detected, and the combined magnetic particle is a combination body of the magnetic particle and the substance to be detected after reaction. The object to be detected which is not combined with the magnetic particles in a reaction manner still affects the accuracy of the detection result in the subsequent detection, and therefore, the object to be detected which is not combined with the magnetic particles in a reaction manner is cleaned, specifically, the object to be detected which is attached to the surface of the combined magnetic particles or is sandwiched between the combined magnetic particles and is not combined with the magnetic particles in a reaction manner is cleaned, so that the object to be detected which is not combined with the magnetic particles in a reaction manner is cleaned, and the influence of the object to be detected which is not combined with the magnetic particles in a reaction manner on the detection accuracy is prevented.
In the existing magnetic particle cleaning device, after a certain amount of mixed liquid of an object to be measured and magnetic particles is added into a reaction container, the object to be measured and the magnetic particles react to form combined magnetic particles, the reaction container is moved to a magnet position, the magnet adsorbs and gathers the combined magnetic particles in the reaction container to one side of the inner wall of the container, liquid in the reaction container is completely sucked through a waste extracting needle, then cleaning liquid is injected after the reaction container leaves the magnet, the gathered combined magnetic particles are dispersed to a uniform mixing state, the reaction container is moved to the same or same side of the magnet position, the liquid in the reaction container is completely sucked through the waste extracting needle after the combined magnetic particles in the reaction container are adsorbed and gathered again, and the combined magnetic particles are cleaned after the actions are repeated for multiple times.
The cleaning device and the cleaning method are easy to clean incompletely, especially the residual objects to be measured attached to the surfaces of the combined magnetic particles or clamped between the combined magnetic particles influence the subsequent detection precision.
Accordingly, there is a need for an improved cleaning apparatus and method to solve the above-mentioned problems.
Disclosure of Invention
In order to solve the above technical problems, an object of the present invention is to provide a magnetic particle cleaning apparatus and a cleaning method for an immunoassay device, which can clean the combined magnetic particles more thoroughly by the distribution of a plurality of magnetic blocks and a plurality of cleaning stations, and the specific technical solution is as follows.
In order to achieve the first object, the present application provides a magnetic particle washing device for an immunoassay device, comprising a reaction vessel initial station, a first washing station, a second washing station, a third washing station and a fourth washing station which are arranged in sequence;
a first magnetic block is arranged on the side edge of the first cleaning station, a first waste extracting needle is arranged at the top of the first cleaning station, and the first magnetic block is close to the first outer wall of the reaction vessel;
a first cleaning liquid injection needle is arranged at the top of the second cleaning station, and a liquid outlet of the first cleaning liquid injection needle is aligned to a first inner wall of the reaction container corresponding to the first outer wall;
a second magnetic block is arranged on the side edge of the third cleaning station, the second magnetic block is close to a second outer wall of the reaction container, and the first outer wall and the second outer wall are arranged oppositely;
a third magnetic block is arranged on the side edge of the fourth cleaning station, a second waste extracting needle is arranged at the top of the fourth cleaning station, and the third magnetic block is close to the first outer wall of the reaction container;
the first waste extraction needle and the second waste extraction needle are respectively lifted through a first lifting mechanism;
the needle head of the first cleaning liquid injection needle is slightly higher than the top end of the reaction container.
Preferably, a fifth cleaning station, a sixth cleaning station and a seventh cleaning station are further included;
a second cleaning liquid injection needle is arranged at the top of the fifth cleaning station, and a liquid outlet of the second cleaning liquid injection needle is aligned to the first inner wall of the reaction container corresponding to the first outer wall;
a fourth magnetic block is arranged on the side edge of the sixth cleaning station and is close to the second outer wall of the reaction vessel;
a fifth magnetic block is arranged on the side edge of the seventh cleaning station, a third waste extracting needle is arranged at the top of the seventh cleaning station, and the fifth magnetic block is close to the first outer wall of the reaction vessel;
the third waste extracting needle is lifted through the first lifting mechanism;
and the needle head of the second cleaning liquid injection needle is slightly higher than the top end of the reaction container.
Preferably, the bottom of the reaction vessel is curved or tapered.
Preferably, the lowest end of the first magnetic block is higher than the lowest end of the reaction vessel, the lowest end of the second magnetic block is higher than the lowest end of the reaction vessel, the lowest end of the third magnetic block is higher than the lowest end of the reaction vessel, the lowest end of the fourth magnetic block is higher than the lowest end of the reaction vessel, and the lowest end of the fifth magnetic block is higher than the lowest end of the reaction vessel.
Preferably, the first magnetic block, the second magnetic block, the third magnetic block, the fourth magnetic block and the fifth magnetic block are all rectangular, circular or irregular in shape.
Preferably, the first magnetic block, the second magnetic block, the third magnetic block, the fourth magnetic block and the fifth magnetic block are formed by splicing a plurality of magnetic blocks respectively.
In order to achieve the second object, the present application provides a magnetic particle cleaning method for an immunoassay device, comprising the steps of:
a reaction solution containing an object to be detected and magnetic particles is configured in a reaction container, and the magnetic particles and the object to be detected are combined to form combined magnetic particles;
moving the reaction vessel to a first cleaning station, adsorbing the combined magnetic particles to a first inner wall of the reaction vessel through a first magnetic block close to a first outer wall of the reaction vessel, descending a first waste extraction needle to the bottom of the reaction vessel, and extracting waste liquid;
moving the reaction container to a second cleaning station, and injecting cleaning liquid into the first inner wall by aligning the liquid outlet of the first cleaning liquid injection needle with the first inner wall;
moving the reaction vessel to a third cleaning station, and adsorbing the combined magnetic particles to a second inner wall of the reaction vessel by a second magnetic block close to a second outer wall of the reaction vessel, wherein the second outer wall and the first outer wall are arranged oppositely;
and moving the reaction vessel to a fourth cleaning station, adsorbing the combined magnetic particles to the first inner wall of the reaction vessel by a third magnetic block close to the first outer wall of the reaction vessel, wherein the third magnetic block is arranged at the diagonal angle of the second magnetic block, adsorbing the combined magnetic particles to the first inner wall from the second inner wall, descending the second waste extraction needle to the bottom of the reaction vessel, and extracting the waste liquid.
Preferably, the method further comprises the following steps:
moving the reaction container to a fifth cleaning station, and injecting cleaning liquid into the liquid outlet of the second cleaning liquid injection needle in alignment with the first inner wall;
moving the reaction vessel to a sixth cleaning station, and adsorbing the combined magnetic particles to the second inner wall of the reaction vessel through a fourth magnetic block close to the second outer wall of the reaction vessel;
and moving the reaction vessel to a seventh cleaning station, adsorbing the combined magnetic particles to the first inner wall of the reaction vessel by a fifth magnetic block close to the first outer wall of the reaction vessel, wherein the fifth magnetic block is arranged at the diagonal angle of the fourth magnetic block, adsorbing the combined magnetic particles to the first inner wall from the second inner wall, and lowering the third waste extraction needle to the bottom of the reaction vessel to extract waste liquid.
Preferably, the bottom of reaction vessel is arc or toper, the least significant end of first magnetic path is higher than reaction vessel's least significant end, the least significant end of second magnetic path is higher than reaction vessel's least significant end, the least significant end of third magnetic path is higher than reaction vessel's least significant end, the least significant end of fourth magnetic path is higher than reaction vessel's least significant end, the least significant end of fifth magnetic path is higher than reaction vessel's least significant end.
Preferably, the moving path of the reaction vessel is a straight line or an arc line.
Through the technical scheme, the technical effects are as follows:
(1) The cleaning device comprises reaction vessel initial stations, a first cleaning station, a second cleaning station, a third cleaning station and a fourth cleaning station which are arranged in sequence, waste liquid is pumped and cleaning liquid is filled through multi-station matching, a liquid outlet of a first cleaning liquid injection needle is aligned to a first inner wall of the reaction vessel corresponding to a first outer wall, the cleaning liquid is directly aligned to magnetic particles attached to the first inner wall after combination, cleaning is more thorough, and precision of follow-up detection is guaranteed.
(2) The second magnetic block and the third magnetic block are arranged in a staggered mode, namely the third magnetic block is located on the opposite side of the second magnetic block, when the reaction container is close to the second magnetic block, the combined magnetic particles are adsorbed on the second inner wall, and when the reaction container moves and is close to the third magnetic block, the combined magnetic particles are adsorbed on the first inner wall; the design enables the combined magnetic particles to be adsorbed to the first inner wall from the second inner wall, the combined magnetic particles to penetrate through the whole reaction container, and in the process that the combined magnetic particles penetrate through the reaction container, the combined magnetic particles are suspended in the cleaning solution and washed again, so that the object to be measured adsorbed on the surfaces of the combined magnetic particles or clamped among the magnetic particles is cleaned.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a perspective view of the cleaning device of the present invention.
FIG. 2 is a schematic top view of the cleaning apparatus of the present invention.
FIG. 3 is a schematic side view of the cleaning apparatus of the present invention.
FIG. 4 is a schematic diagram of a magnetic block of the present invention.
FIG. 5 is a flow chart of the cleaning method of the present invention.
FIG. 6 is a flow chart of the cleaning method of the present invention.
Wherein, 1, a first magnetic block; 2. a first waste extraction needle; 3. a reaction vessel; 31. a first outer wall; 32. a first inner wall; 33. a second outer wall; 34. a second inner wall; 4. a first cleaning solution injection needle; 5. a second magnetic block; 6. a third magnetic block; 7. a second waste extracting needle; 8. a second cleaning solution injection needle; 9. a fourth magnetic block; 10. a fifth magnetic block; 11. thirdly, waste needle extraction; 12. and (6) splicing the lines.
Detailed Description
The technical solutions of the present invention will be described in detail with reference to specific examples, which should be understood as illustrative only and not as limiting the scope of the invention, and various equivalent modifications of the invention will fall within the scope of the appended claims of the present application after reading the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.
Example 1
Fig. 1 to 3 show one embodiment of a magnetic particle washing apparatus for an immunoassay device according to the present invention.
As shown in fig. 1 to 3, the magnetic particle cleaning device for an immunoassay device includes a reaction vessel initial station, a first cleaning station, a second cleaning station, a third cleaning station, and a fourth cleaning station, which are arranged in sequence; a first magnetic block 1 is arranged on the side edge of the first cleaning station, a first waste extracting needle 2 is arranged at the top of the first cleaning station, and the first magnetic block 1 is close to a first outer wall 31 of a reaction container 3; a first cleaning liquid injection needle 4 is arranged at the top of the second cleaning station, and a liquid outlet of the first cleaning liquid injection needle 4 is aligned to a first inner wall 32 of the reaction container 3 corresponding to the first outer wall 31; a second magnetic block 5 is arranged on the side edge of the third cleaning station, the second magnetic block 5 is close to a second outer wall 33 of the reaction container 3, and the first outer wall 31 and the second outer wall 33 are oppositely arranged; a third magnetic block 6 is arranged on the side edge of the fourth cleaning station, a second waste extracting needle 7 is arranged at the top of the fourth cleaning station, and a third magnetic block 8 is close to a first outer wall 31 of the reaction vessel; the first waste extraction needle 2 and the second waste extraction needle 7 are respectively lifted through a first lifting mechanism; the needle head of the first cleaning liquid injection needle 4 is slightly higher than the top end of the reaction container 3.
Specifically, referring to fig. 1 to 3, in order to thoroughly clean the object to be measured adsorbed on the surface of the combined magnetic particles or between the combined magnetic particles, at present, the same magnetic block is close to or away from the reaction container, so that the combined magnetic particles are adsorbed or released to the cleaning solution, and the cleaning solution is pumped and filled for multiple times, so that the cleaning efficiency is low, the movement range of the combined magnetic particles in the cleaning solution is small, the combined magnetic particles are not thoroughly cleaned in the cleaning solution, and the object to be measured is also partially adsorbed on the surface of the combined magnetic particles or is sandwiched between the combined magnetic particles. In order to solve the technical problem, the embodiment is provided with a plurality of cleaning stations, so that the reaction easily passes through an initial station, a first cleaning station, a second cleaning station, a third cleaning station and a fourth cleaning station in sequence; specifically, the initial station is a reaction station of the magnetic particles and the object to be detected, and the combined magnetic particles are formed after the reaction is finished; moving the reaction vessel 3 to a first cleaning station, adsorbing the combined magnetic particles to a first inner wall 32 by a first magnetic block 1, descending a first waste extraction needle 2, completely absorbing the waste liquid by the first waste extraction needle 2, and cleaning part of the waste liquid at the first cleaning station; in order to further clean the object to be detected, the reaction container 3 is moved to a second cleaning station, the liquid outlet of the first cleaning liquid injection needle 4 is aligned to the first inner wall 32 of the reaction container 3 corresponding to the first outer wall 31 for cleaning, the combined magnetic particles attached to the first inner wall 32 are cleaned, the cleaning angle is aligned to the adsorbed surface of the combined magnetic particles and is 10-30 degrees, the cleaning angle has a cleaning effect on the combined magnetic particles, in order to prevent the first cleaning liquid injection needle 4 from being polluted, the liquid outlet of the first cleaning liquid injection needle 4 is slightly higher than the top end of the reaction container 3, and the cleaning force is adjusted through a liquid injection pump; in order to increase the moving range of the combined magnetic particles in the reaction container 3, the reaction container 3 is moved to a third cleaning station, the combined magnetic particles are adsorbed to the second inner wall 34 through the second magnetic block 5, neither a waste extraction needle nor a filling needle is arranged at the position, and the suspended combined magnetic particles are adsorbed to the second inner wall 34 through the station, so that the combined magnetic particles are cleaned in the process of moving to the second inner wall 34; in order to further increase the moving range of the combined magnetic particles in the reaction vessel 3, the reaction vessel 3 is moved to a fourth cleaning station, the combined magnetic particles are adsorbed to the first inner wall 32 through a third magnetic block 6, the second magnetic block 5 and the third magnetic block 6 are arranged in a staggered manner, namely the third magnetic block 6 is positioned diagonally opposite to the second magnetic block 5, when the reaction vessel 3 is close to the second magnetic block 5, the combined magnetic particles are adsorbed to the second inner wall 34, and when the reaction vessel 3 is moved and close to the third magnetic block 6, the combined magnetic particles are adsorbed to the first inner wall 32; the design makes the combined magnetic particles be absorbed to the first inner wall 32 from the second inner wall 34, makes the combined magnetic particles pass through the whole reaction container 3, and makes the combined magnetic particles suspended in the cleaning solution and washed again in the process that the combined magnetic particles pass through the reaction container 3, so that the object to be measured absorbed on the surface of the combined magnetic particles or sandwiched between the combined magnetic particles is cleaned.
As a preferred embodiment, referring to fig. 2, a fifth cleaning station, a sixth cleaning station and a seventh cleaning station are also included; a second cleaning liquid injection needle 8 is arranged at the top of the fifth cleaning station, and a liquid outlet of the second cleaning liquid injection needle 8 is aligned to a first inner wall 32 of the reaction container 3 corresponding to the first outer wall 31; a fourth magnetic block 9 is arranged on the side edge of the sixth cleaning station, and the fourth magnetic block 9 is close to the second outer wall 33 of the reaction vessel 3; a fifth magnetic block 10 is arranged on the side edge of the seventh cleaning station, a third waste extracting needle 11 is arranged at the top of the seventh cleaning station, and the fifth magnetic block 10 is close to the first outer wall 31 of the reaction vessel 3; the third waste extracting needle 11 is lifted through the first lifting mechanism; the needle head of the second cleaning liquid injection needle 8 is slightly higher than the top end of the reaction vessel 3.
Specifically, the setting function of the fifth cleaning station is the same as that of the second cleaning station, the setting function of the sixth cleaning station is the same as that of the third cleaning station, and the setting function of the seventh cleaning station is the same as that of the fourth cleaning station; that is, the second cleaning station, the third cleaning station and the fourth cleaning station are a cleaning cycle, and the fifth cleaning station, the sixth cleaning station and the seventh cleaning station are the same cleaning cycle, so that the object to be measured adsorbed on the surfaces of the bonded magnetic particles or sandwiched between the bonded magnetic particles is thoroughly cleaned by the cleaning in the two cleaning cycles.
It should be further noted that, the first lifting mechanism is to lift the first waste extraction needle 2, the second waste extraction needle 7 or the third waste extraction needle 11, when the first waste extraction needle 2, the second waste extraction needle 7 or the third waste extraction needle 11 is lowered, the needle tip portion of the first waste extraction needle 2, the second waste extraction needle 7 or the third waste extraction needle 11 contacts the bottommost portion of the reaction container 3, the waste extraction liquid is lifted up, and the first waste extraction needle 2, the second waste extraction needle 7 or the third waste extraction needle 11 leaves the reaction container 3.
As a preferred embodiment, referring to fig. 2, the bottom of the reaction vessel 3 is curved or tapered. In particular, the curved or tapered bottom allows the reaction vessel 3 to have a minimum, allowing small amounts of residual waste liquid to be sucked clean.
As a preferred embodiment, referring to fig. 3, the lowest end of the first magnetic block 1 is higher than the lowest end of the reaction vessel 3, the lowest end of the second magnetic block 5 is higher than the lowest end of the reaction vessel 3, the lowest end of the third magnetic block 6 is higher than the lowest end of the reaction vessel 3, the lowest end of the fourth magnetic block 9 is higher than the lowest end of the reaction vessel 3, and the lowest end of the fifth magnetic block 10 is higher than the lowest end of the reaction vessel 3. Specifically, the lowest ends of the first magnetic block 1, the second magnetic block 5, the third magnetic block 6, the fourth magnetic block 9 and the fifth magnetic block 10 are all higher than the lowest end of the reaction vessel 3, so that the combined magnetic particles are far away from the lowest end of the reaction vessel 3, and the combined magnetic particles are prevented from being drawn away by the waste liquid drawing needle when the waste liquid is drawn.
As a preferred embodiment, the first magnetic block 1, the second magnetic block 5, the third magnetic block 6, the fourth magnetic block 9 and the fifth magnetic block 10 are all rectangular, circular or irregular in shape. Specifically, no matter the shape of the magnetic block is circular or rectangular, the combined magnetic particles will be gathered and distributed on the corresponding inner wall of the magnetic block.
As a preferred embodiment, referring to fig. 4, the first magnetic block 1, the second magnetic block 5, the third magnetic block 6, the fourth magnetic block 9 and the fifth magnetic block 10 are respectively formed by splicing a plurality of magnetic blocks; referring to fig. 4, when the three magnetic blocks are spliced, the splicing line 12 is vertically located on the first magnetic block 1, the second magnetic block 5, the third magnetic block 6, the fourth magnetic block 9 and the fifth magnetic block 10. Specifically, for the spliced magnetic block, the magnetic field at the splicing line 12 is strongest, most of the combined magnetic particles are gathered at the splicing line 12, and the increased magnetic field is helpful for the gathering speed of the combined magnetic particles, so that the cleaning efficiency is improved.
Example 2
Fig. 5 shows a specific embodiment of the magnetic particle washing method of the immunoassay device of the present invention.
The magnetic particle cleaning method of the immunoassay device, referring to the flow chart of fig. 5, comprises the following steps:
s1: a reaction solution containing an object to be measured and magnetic particles is disposed in a reaction vessel, and the magnetic particles are bonded to the object to be measured to form bonded magnetic particles. Specifically, the analyte is one of an antigen, a hapten, an antibody, a hormone and the like, the analyte reacts with the magnetic particles to form combined magnetic particles, the combined magnetic particles are the basis for subsequent detection, the combined magnetic particles need to be cleaned, and if the analyte adheres to the surfaces of the combined magnetic particles, the accuracy of the subsequent detection is affected.
S2: the reaction vessel is moved to a first cleaning station, the combined magnetic particles are adsorbed to the first inner wall of the reaction vessel through a first magnetic block close to the first outer wall of the reaction vessel, the first waste extraction needle is descended to the bottom of the reaction vessel, and waste liquid is extracted. Specifically, the reaction vessel is moved to a first cleaning station, the first magnetic block adsorbs the combined magnetic particles to a first inner wall, the first waste extraction needle descends, the waste liquid is completely absorbed through the first waste extraction needle, and part of the waste liquid is cleaned at the first cleaning station.
S3: and moving the reaction vessel to a second cleaning station, and aligning the liquid outlet of the first cleaning liquid injection needle with the first inner wall to inject the cleaning liquid. Specifically, the liquid outlet of the first cleaning liquid injection needle is aligned to the first inner wall of the reaction container corresponding to the first outer wall for washing, the combined magnetic particles attached to the first inner wall are washed, the washing angle is aligned to the adsorbed surface of the combined magnetic particles and is 10-30 degrees, the washing angle has a washing effect on the combined magnetic particles, and in order to prevent the first cleaning liquid injection needle from being polluted, the liquid outlet of the first cleaning liquid injection needle is slightly higher than the top end of the reaction container, and the washing force is adjusted through the liquid injection pump.
S4: and moving the reaction vessel to a third cleaning station, and adsorbing the combined magnetic particles to a second inner wall of the reaction vessel by a second magnetic block close to the second outer wall of the reaction vessel, wherein the second outer wall and the first outer wall are oppositely arranged. Specifically, in order to increase the moving range of the combined magnetic particles in the reaction vessel, the reaction vessel is moved to a third cleaning station, the combined magnetic particles are adsorbed to the second inner wall through the second magnetic block, neither a waste extraction needle nor a filling needle is arranged at the position, and the suspended combined magnetic particles are adsorbed to the second inner wall through the station, so that the combined magnetic particles are cleaned in the process of moving to the second inner wall.
S5: and moving the reaction vessel to a fourth cleaning station, adsorbing the combined magnetic particles to the first inner wall of the reaction vessel by a third magnetic block close to the first outer wall of the reaction vessel, wherein the third magnetic block is arranged at the diagonal angle of the second magnetic block, adsorbing the combined magnetic particles to the first inner wall from the second inner wall, descending the second waste extraction needle to the bottom of the reaction vessel, and extracting the waste liquid. Specifically, in order to further increase the moving range of the combined magnetic particles in the reaction vessel, the reaction vessel is moved to a fourth cleaning station, the combined magnetic particles are adsorbed to the first inner wall by a third magnetic block, the second magnetic block and the third magnetic block are arranged in a staggered manner, that is, the third magnetic block is positioned diagonally opposite to the second magnetic block, when the reaction vessel is close to the second magnetic block, the combined magnetic particles are adsorbed to the second inner wall, and when the reaction vessel is moved and close to the third magnetic block, the combined magnetic particles are adsorbed to the first inner wall.
As a preferred embodiment, referring to the flowchart of fig. 6, the following steps are further included:
s6: and moving the reaction vessel to a fifth cleaning station, and aligning a liquid outlet of a second cleaning liquid injection needle with a first inner wall to inject cleaning liquid. Specifically, the fifth cleaning station and the second cleaning station are arranged to function in the same manner.
S7: and moving the reaction vessel to a sixth cleaning station, and adsorbing the combined magnetic particles to the second inner wall of the reaction vessel by a fourth magnetic block close to the second outer wall of the reaction vessel. Specifically, the sixth cleaning station has the same setting function as the third cleaning station.
S8: and moving the reaction vessel to a seventh cleaning station, adsorbing the combined magnetic particles to the first inner wall of the reaction vessel by a fifth magnetic block close to the first outer wall of the reaction vessel, wherein the fifth magnetic block is arranged at the diagonal angle of the fourth magnetic block, adsorbing the combined magnetic particles to the first inner wall from the second inner wall, descending the third waste extraction needle to the bottom of the reaction vessel, and extracting the waste liquid. Specifically, the seventh cleaning station and the fourth cleaning station are arranged to function in the same manner.
Through the embodiment, the second cleaning station, the third cleaning station and the fourth cleaning station are a cleaning cycle, the fifth cleaning station, the sixth cleaning station and the seventh cleaning station are the same cleaning cycle, and through the cleaning of the two cleaning cycles, the object to be measured adsorbed on the surfaces of the combined magnetic particles or clamped between the combined magnetic particles is thoroughly cleaned.
As a preferred embodiment, referring to fig. 3, the bottom of the reaction vessel 3 is arc-shaped or conical, the lowest end of the first magnetic block 1 is higher than the lowest end of the reaction vessel 3, the lowest end of the second magnetic block 5 is higher than the lowest end of the reaction vessel 3, the lowest end of the third magnetic block 6 is higher than the lowest end of the reaction vessel 3, the lowest end of the fourth magnetic block 9 is higher than the lowest end of the reaction vessel 3, and the lowest end of the fifth magnetic block 10 is higher than the lowest end of the reaction vessel 3. In particular, the curved or conical bottom allows the reaction vessel 3 to have a minimum, allowing a small amount of residual waste liquid to be sucked clean; the lowest ends of the first magnetic block 1, the second magnetic block 5, the third magnetic block 6, the fourth magnetic block 9 and the fifth magnetic block 10 are all higher than the lowest end of the reaction vessel 3, so that the combined magnetic particles are far away from the lowest end of the reaction vessel 3, and the combined magnetic particles are prevented from being drawn away by the waste liquid drawing needle when waste liquid is drawn.
As a preferred embodiment, the moving path of the reaction vessel 3 is a straight line or an arc line. Specifically, according to the volume of the cleaning device and the specific detection flow of the immunoassay device, the moving path of the reaction vessel 3 may be cleaned in the linear moving process or in the arc moving process.
Claims (10)
1. A magnetic particle cleaning apparatus for an immunoassay device, characterized in that,
the device comprises a reaction vessel initial station, a first cleaning station, a second cleaning station, a third cleaning station and a fourth cleaning station which are arranged in sequence;
a first magnetic block is arranged on the side edge of the first cleaning station, a first waste extracting needle is arranged at the top of the first cleaning station, and the first magnetic block is close to the first outer wall of the reaction vessel;
a first cleaning liquid injection needle is arranged at the top of the second cleaning station, and a liquid outlet of the first cleaning liquid injection needle is aligned to a first inner wall of the reaction container corresponding to the first outer wall;
a second magnetic block is arranged on the side edge of the third cleaning station, the second magnetic block is close to a second outer wall of the reaction vessel, and the first outer wall and the second outer wall are arranged oppositely;
a third magnetic block is arranged on the side edge of the fourth cleaning station, a second waste extracting needle is arranged at the top of the fourth cleaning station, and the third magnetic block is close to the first outer wall of the reaction vessel;
the first waste extraction needle and the second waste extraction needle are respectively lifted through a first lifting mechanism;
the needle head of the first cleaning liquid injection needle is slightly higher than the top end of the reaction container.
2. The magnetic particle washing device for an immunoassay apparatus according to claim 1, further comprising a fifth washing station, a sixth washing station and a seventh washing station;
a second cleaning liquid injection needle is arranged at the top of the fifth cleaning station, and a liquid outlet of the second cleaning liquid injection needle is aligned to the first inner wall of the reaction vessel corresponding to the first outer wall;
a fourth magnetic block is arranged on the side edge of the sixth cleaning station and is close to the second outer wall of the reaction vessel;
a fifth magnetic block is arranged on the side edge of the seventh cleaning station, a third waste extraction needle is arranged at the top of the seventh cleaning station, and the fifth magnetic block is close to the first outer wall of the reaction vessel;
the third waste extracting needle is lifted through the first lifting mechanism;
and the needle head of the second cleaning liquid injection needle is slightly higher than the top end of the reaction container.
3. The magnetic particle washing apparatus for an immunological analysis device according to claim 1 or 2, wherein the bottom of the reaction vessel is arc-shaped or tapered.
4. The magnetic particle cleaning device for the immunoassay device as set forth in claim 3, wherein the lowest end of the first magnetic block is higher than the lowest end of the reaction vessel, the lowest end of the second magnetic block is higher than the lowest end of the reaction vessel, the lowest end of the third magnetic block is higher than the lowest end of the reaction vessel, the lowest end of the fourth magnetic block is higher than the lowest end of the reaction vessel, and the lowest end of the fifth magnetic block is higher than the lowest end of the reaction vessel.
5. The magnetic particle cleaning device for the immunoassay equipment as defined in claim 4, wherein the first magnetic block, the second magnetic block, the third magnetic block, the fourth magnetic block and the fifth magnetic block are all rectangular, circular or irregular in shape.
6. The magnetic particle cleaning device for the immunoassay equipment as recited in claim 5, wherein the first magnetic block, the second magnetic block, the third magnetic block, the fourth magnetic block and the fifth magnetic block are respectively formed by splicing a plurality of magnetic blocks.
7. The magnetic particle cleaning method of the immunoassay device is characterized by comprising the following steps:
a reaction solution containing an object to be detected and magnetic particles is configured in a reaction container, and the magnetic particles and the object to be detected are combined to form combined magnetic particles;
moving the reaction vessel to a first cleaning station, adsorbing the combined magnetic particles to the first inner wall of the reaction vessel through a first magnetic block close to the first outer wall of the reaction vessel, descending a first waste extraction needle to the bottom of the reaction vessel, and extracting waste liquid;
moving the reaction container to a second cleaning station, and injecting cleaning liquid into the first inner wall by aligning the liquid outlet of the first cleaning liquid injection needle with the liquid outlet of the first inner wall;
moving the reaction vessel to a third cleaning station, and adsorbing the combined magnetic particles to a second inner wall of the reaction vessel by a second magnetic block close to a second outer wall of the reaction vessel, wherein the second outer wall and the first outer wall are oppositely arranged;
and moving the reaction vessel to a fourth cleaning station, adsorbing the combined magnetic particles to the first inner wall of the reaction vessel by a third magnetic block close to the first outer wall of the reaction vessel, wherein the third magnetic block is arranged at the diagonal angle of the second magnetic block, adsorbing the combined magnetic particles to the first inner wall from the second inner wall, descending the second waste extraction needle to the bottom of the reaction vessel, and extracting the waste liquid.
8. The magnetic particle washing method of an immunoassay apparatus according to claim 7, further comprising the steps of:
moving the reaction container to a fifth cleaning station, and injecting cleaning liquid into the liquid outlet of the second cleaning liquid injection needle in alignment with the first inner wall;
moving the reaction vessel to a sixth cleaning station, and adsorbing the combined magnetic particles to the second inner wall of the reaction vessel through a fourth magnetic block close to the second outer wall of the reaction vessel;
and moving the reaction vessel to a seventh cleaning station, adsorbing the combined magnetic particles to the first inner wall of the reaction vessel by a fifth magnetic block close to the first outer wall of the reaction vessel, wherein the fifth magnetic block is arranged at the diagonal angle of the fourth magnetic block, adsorbing the combined magnetic particles to the first inner wall from the second inner wall, descending the third waste extraction needle to the bottom of the reaction vessel, and extracting the waste liquid.
9. The method for washing magnetic particles of an immunoassay device according to claim 8, wherein the bottom of the reaction vessel is arc-shaped or tapered, the lowest end of the first magnetic block is higher than the lowest end of the reaction vessel, the lowest end of the second magnetic block is higher than the lowest end of the reaction vessel, the lowest end of the third magnetic block is higher than the lowest end of the reaction vessel, the lowest end of the fourth magnetic block is higher than the lowest end of the reaction vessel, and the lowest end of the fifth magnetic block is higher than the lowest end of the reaction vessel.
10. The magnetic particle washing method of an immunoassay apparatus according to claim 9, wherein the moving path of the reaction vessel is a straight line or an arc line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210922251.XA CN115156215A (en) | 2022-08-02 | 2022-08-02 | Magnetic particle cleaning device and cleaning method for immunoassay device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210922251.XA CN115156215A (en) | 2022-08-02 | 2022-08-02 | Magnetic particle cleaning device and cleaning method for immunoassay device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115156215A true CN115156215A (en) | 2022-10-11 |
Family
ID=83477601
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210922251.XA Pending CN115156215A (en) | 2022-08-02 | 2022-08-02 | Magnetic particle cleaning device and cleaning method for immunoassay device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115156215A (en) |
Citations (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07318559A (en) * | 1994-05-25 | 1995-12-08 | Mitsubishi Chem Corp | Immunochemical measuring apparatus |
| JPH09257796A (en) * | 1996-03-19 | 1997-10-03 | Olympus Optical Co Ltd | Analyser and analyzing method |
| JP2001116752A (en) * | 1999-10-19 | 2001-04-27 | Hitachi Ltd | Magnetic separation method of magnetic particle and chamical analyzer |
| CN1735806A (en) * | 2002-11-07 | 2006-02-15 | 株式会社三菱化学药得论 | Magnetic material for collecting magnetic particles and utilization thereof |
| JP2010032386A (en) * | 2008-07-29 | 2010-02-12 | Olympus Corp | Reaction table, analyzer, and stirring method |
| CN202101998U (en) * | 2011-06-16 | 2012-01-04 | 深圳市国赛生物技术有限公司 | Full-automatic chemiluminescence test device |
| CN202110180U (en) * | 2011-06-22 | 2012-01-11 | 深圳市国赛生物技术有限公司 | Integrated device capable of magnetically stirring, cleaning and separating |
| CN204165989U (en) * | 2014-11-06 | 2015-02-18 | 南京基蛋生物科技有限公司 | A kind of magnetic particle cleaning is separated settling flux device |
| CN108015071A (en) * | 2017-12-28 | 2018-05-11 | 上海裕隆生物科技有限公司 | A kind of magnetic microparticle chemiluminescence analyzer cleaning device and method |
| CN208082677U (en) * | 2018-05-08 | 2018-11-13 | 深圳迎凯生物科技有限公司 | Automatic cleaning separator |
| CN108856112A (en) * | 2018-03-19 | 2018-11-23 | 迈克医疗电子有限公司 | Magnetic bead cleaning method and device |
| CN208303355U (en) * | 2018-02-08 | 2019-01-01 | 成都深迈瑞医疗电子技术研究院有限公司 | Magnetic bead cleans separator and chemical illumination immunity analysis instrument |
| CN109865704A (en) * | 2019-04-10 | 2019-06-11 | 安图实验仪器(郑州)有限公司 | Magnetic bead cleaning device suitable for in-vitro diagnosis equipment |
| CN110823669A (en) * | 2019-11-08 | 2020-02-21 | 广州科方生物技术股份有限公司 | Magnetic separation cleaning method |
| CN210665759U (en) * | 2019-09-24 | 2020-06-02 | 重庆科斯迈生物科技有限公司 | Washing channel for chemiluminescence immunoassay |
| CN111279197A (en) * | 2017-12-07 | 2020-06-12 | 株式会社日立高新技术 | Magnetic separation method and automatic analyzer |
| CN111841877A (en) * | 2019-10-08 | 2020-10-30 | 潍坊市康华生物技术有限公司 | Device for reducing magnetic bead loss in magnetic separation and use method |
| CN112088311A (en) * | 2018-03-15 | 2020-12-15 | 株式会社日立高新技术 | Automatic analyzer |
-
2022
- 2022-08-02 CN CN202210922251.XA patent/CN115156215A/en active Pending
Patent Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07318559A (en) * | 1994-05-25 | 1995-12-08 | Mitsubishi Chem Corp | Immunochemical measuring apparatus |
| JPH09257796A (en) * | 1996-03-19 | 1997-10-03 | Olympus Optical Co Ltd | Analyser and analyzing method |
| JP2001116752A (en) * | 1999-10-19 | 2001-04-27 | Hitachi Ltd | Magnetic separation method of magnetic particle and chamical analyzer |
| CN1735806A (en) * | 2002-11-07 | 2006-02-15 | 株式会社三菱化学药得论 | Magnetic material for collecting magnetic particles and utilization thereof |
| CN101398428A (en) * | 2002-11-07 | 2009-04-01 | 株式会社三菱化学药得论 | Magnetic material for collecting magnetic particles and utilization thereof |
| JP2010032386A (en) * | 2008-07-29 | 2010-02-12 | Olympus Corp | Reaction table, analyzer, and stirring method |
| CN202101998U (en) * | 2011-06-16 | 2012-01-04 | 深圳市国赛生物技术有限公司 | Full-automatic chemiluminescence test device |
| CN202110180U (en) * | 2011-06-22 | 2012-01-11 | 深圳市国赛生物技术有限公司 | Integrated device capable of magnetically stirring, cleaning and separating |
| CN204165989U (en) * | 2014-11-06 | 2015-02-18 | 南京基蛋生物科技有限公司 | A kind of magnetic particle cleaning is separated settling flux device |
| CN111279197A (en) * | 2017-12-07 | 2020-06-12 | 株式会社日立高新技术 | Magnetic separation method and automatic analyzer |
| CN108015071A (en) * | 2017-12-28 | 2018-05-11 | 上海裕隆生物科技有限公司 | A kind of magnetic microparticle chemiluminescence analyzer cleaning device and method |
| CN208303355U (en) * | 2018-02-08 | 2019-01-01 | 成都深迈瑞医疗电子技术研究院有限公司 | Magnetic bead cleans separator and chemical illumination immunity analysis instrument |
| CN112088311A (en) * | 2018-03-15 | 2020-12-15 | 株式会社日立高新技术 | Automatic analyzer |
| CN108856112A (en) * | 2018-03-19 | 2018-11-23 | 迈克医疗电子有限公司 | Magnetic bead cleaning method and device |
| CN208082677U (en) * | 2018-05-08 | 2018-11-13 | 深圳迎凯生物科技有限公司 | Automatic cleaning separator |
| CN109865704A (en) * | 2019-04-10 | 2019-06-11 | 安图实验仪器(郑州)有限公司 | Magnetic bead cleaning device suitable for in-vitro diagnosis equipment |
| CN210665759U (en) * | 2019-09-24 | 2020-06-02 | 重庆科斯迈生物科技有限公司 | Washing channel for chemiluminescence immunoassay |
| CN111841877A (en) * | 2019-10-08 | 2020-10-30 | 潍坊市康华生物技术有限公司 | Device for reducing magnetic bead loss in magnetic separation and use method |
| CN110823669A (en) * | 2019-11-08 | 2020-02-21 | 广州科方生物技术股份有限公司 | Magnetic separation cleaning method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN211255881U (en) | Full-automatic nucleic acid extraction system | |
| CN203737677U (en) | Magnetic bead swimming motility washing device for chemical luminescence immunity analyzer | |
| CN110628569A (en) | Full-automatic nucleic acid extraction system | |
| CN108982195A (en) | A kind of full-automatic immunomagnetic beads method purifying instrument | |
| CA3019181C (en) | Dmf method and system for concentrating analyte from large volumes into smaller volumes using magnetic microparticles | |
| CN115156215A (en) | Magnetic particle cleaning device and cleaning method for immunoassay device | |
| CN214585496U (en) | Sampling needle cleaning structure for multi-channel dry-type fluorescence immunoassay analyzer | |
| JP2014070915A (en) | Liquid sucking method, and solid phase washing method using the same | |
| CN207623356U (en) | A kind of wash mill for chemiluminescence immunoassay detection | |
| CN108082619A (en) | A kind of automatic liquid separation encapsulates labeling device | |
| CN115254834B (en) | Magnetic particle cleaning device and method for immunoassay equipment | |
| US9664675B2 (en) | Automated analyzer | |
| CN209784383U (en) | Chemiluminescence immunodetection system | |
| CN115707972A (en) | Magnetic separation cleaning, mixing and measuring device and sample analyzer | |
| CN202892972U (en) | Acoustic surface wave acceleration droplet-droplet micro-extraction device | |
| CN206671205U (en) | A kind of chemicals dosing plant of online water analysis instrument | |
| JP2000500858A (en) | Method and apparatus for analyzing volatile substances in gas | |
| CN218601291U (en) | Buffer solution assembly of sample analyzer and sample analyzer | |
| CN214539657U (en) | Sampling device for biological reagent | |
| JPH0445066B2 (en) | ||
| CN206281697U (en) | A kind of 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments | |
| US20230372926A1 (en) | Microfluidic devices and processes | |
| CN218157928U (en) | Single-person magnetic particle chemiluminescence reagent strip | |
| CN216192370U (en) | Nucleic acid extraction device of magnetic bead method liquid separation extraction instrument | |
| JPS59120960A (en) | Automatic inserting and guiding method of suction nozzle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |