CN111841877A - Device for reducing magnetic bead loss in magnetic separation and use method - Google Patents
Device for reducing magnetic bead loss in magnetic separation and use method Download PDFInfo
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- CN111841877A CN111841877A CN201910947732.4A CN201910947732A CN111841877A CN 111841877 A CN111841877 A CN 111841877A CN 201910947732 A CN201910947732 A CN 201910947732A CN 111841877 A CN111841877 A CN 111841877A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
Abstract
The invention provides a device for reducing loss of magnetic beads during magnetic separation, which comprises a reaction disc, wherein the reaction disc is provided with a bracket capable of rotating in the reaction disc, a reaction cup is arranged on the bracket, the reaction disc is provided with a magnet bracket, the magnet bracket is provided with magnets, the reaction cup corresponds to the magnet bracket in position, when the reaction cup moves to the position of the magnet bracket through the bracket, a gap is reserved between the magnet bracket and the reaction cup, the bottom of the reaction disc is provided with a blending component, and the blending component corresponds to the position of the reaction cup arranged on the bracket. This device can wash the magnetic bead thoroughly when wasing the magnetic separation, and can effectively reduce the loss of magnetic bead among the cleaning process, and the effect is better, is suitable for popularization and application.
Description
Technical Field
The invention relates to the field of magnetic-liquid separation, in particular to a device for reducing magnetic bead loss in magnetic separation and a using method thereof.
Background
Cleaning magnetic-liquid separation is an important link before chemiluminescence detection, and the structure of the magnetic-liquid separation directly influences the detection result and the stability of the detection result. The impurities which are not reacted in the reaction cup can be pumped away, only the well-reacted combination is left, and the following defects can occur in the prior design:
1. The magnetic beads are sucked away by a washing liquid needle in the washing process;
2. the cleaning needle can cause carrying pollution in the cleaning process, and the experimental result is influenced;
3. the magnetic beads are not thoroughly washed clean during the washing process.
Disclosure of Invention
The invention aims to overcome the defects of the traditional technology and provide the device for reducing the loss of the magnetic beads during magnetic separation, and the device can effectively reduce the loss of the magnetic beads during the cleaning process and effectively solve the technical problems.
The aim of the invention is achieved by the following technical measures: the utility model provides a device of magnetic bead loss when reducing magnetic separation, includes the reaction disc, the reaction disc is installed and can be at the bracket of reaction disc internal rotation, install reaction cup on the bracket, reaction disc fixed mounting has the magnet support, magnet support installs magnet, reaction cup and magnet support position are corresponding, and when reaction cup moved the position of magnet support through the bracket, reaction cup was located the magnetic force district of magnet, left the clearance between magnet support and the reaction cup, the mixing subassembly is installed to the reaction disc bottom, the mixing subassembly is corresponding with the reaction cup position of bracket installation.
Through the setting, annotate the liquid in to the reaction cup through wasing the notes liquid needle, through when the bracket rotates to the magnet support position, wash the magnetic bead in with the reaction cup through the mixing subassembly with liquid mixing, be provided with between the magnet of taking magnet, can adsorb the magnetic bead in the reaction cup at the cup inner wall, can not absorb the magnetic bead in the cup when extracting the waste liquid in the cup away, can effectively reduce the loss of magnetic bead.
As a preferred scheme, the mixing subassembly is including fixing the mixing motor support at the reaction disc lateral wall, mixing motor support mounting has the mixing motor, mixing seat support is installed to the reaction disc bottom, mixing seat support mounting has the transmission shaft, the mixing head is installed to the one end of transmission shaft, the mixing head is corresponding with the position of reaction cup, the mixing motor is connected with the transmission shaft transmission.
Through the arrangement, when the bracket moves to the upper part of the mixing component, the bottom of the reaction cup is contacted with the mixing head, and the mixing head can drive the reaction cup to move when rotating, so that the effect of mixing and cleaning magnetic beads and liquid in the reaction cup is achieved.
As a preferred scheme, the drive wheel is installed to mixing motor bottom, mixing motor support bottom is installed from the driving wheel, synchronous pulley is installed to the one end that the mixing head was kept away from to the transmission shaft, install the drive belt on drive wheel, the follow driving wheel and the synchronous pulley.
As a preferred scheme, the mixing head comprises an upper part and a lower part, the upper part and the lower part are eccentrically arranged, a spherical groove is formed in the top surface of the upper part, and the bottom of the reaction cup is located in the middle of the groove when the reaction cup corresponds to the mixing head. The upper part and the lower part of the mixing head are both cylindrical, the spherical groove is positioned in the middle of the upper part of the mixing head, when the mixing head rotates, the upper part and the lower part are eccentrically arranged, the bottom of the reaction cup can move upwards along the inner wall of the groove and fall down under the action of gravity, and in the repeated process, the magnetic beads in the reaction cup can be fully mixed with liquid, so that the purpose of cleaning the magnetic beads is achieved.
As a preferred scheme, the fixed frame is fixedly arranged on the side wall of the reaction disc, the driving motor is installed at the bottom of the fixed frame, the movable plate is installed in the fixed frame and is in transmission connection with the driving motor, and the movable plate is provided with the cleaning assembly.
As a preferred scheme, the mount includes bottom plate, roof and curb plate, curb plate and mount fixed connection, roof and bottom plate parallel arrangement are at the both ends of curb plate, driving motor installs in the bottom plate bottom, be provided with the traveller between roof and the bottom plate, traveller slidable mounting has the fly leaf, the fly leaf is provided with the screw hole, and the screw rod is installed to first motor, the screw rod passes screw hole and fly leaf threaded connection.
Through the arrangement, the driving motor can drive the cleaning component fixed on the movable plate to move up and down when driving the screw rod to rotate, and the cleaning liquid injection needle and the cleaning liquid extraction needle are conveniently inserted into the reaction cup to perform liquid injection or liquid extraction work.
As a preferred scheme, the cleaning assembly comprises a guide shaft arranged on the movable plate, a cleaning carriage is arranged at the top of the guide shaft, and a cleaning liquid injection needle and a cleaning liquid extraction needle are arranged on the cleaning carriage.
As a preferable scheme, the cleaning liquid injection needles and the cleaning liquid extraction needles are respectively provided with three needles, the positions of the cleaning liquid injection needles and the positions of the cleaning liquid extraction needles correspond to the positions of the bracket, and the three cleaning liquid injection needles and the three cleaning liquid extraction needles are arranged on the cleaning carriage in a crossed manner.
Preferably, the reaction disc is provided with a light shield, and the light shield corresponds to the cleaning assembly in position. The light shield is provided with a plurality of plate holes, and when the reaction cup in the bracket moves to the bottom of the light shield, the plate holes correspond to the reaction cup.
Through the arrangement, when the reaction cup moves to the bottom of the light shield, the cleaning liquid injection needle and the cleaning liquid extraction needle can penetrate through the light shield and be inserted into the reaction cup for liquid injection or liquid extraction.
A method for using the device for reducing the loss of the magnetic beads during magnetic separation comprises the following steps that along with the anticlockwise rotation of a bracket on a reaction disc, reaction cups in the bracket also rotate one by one, the position of a first cleaning liquid injection needle is rotated, a cleaning assembly moves to bottom liquid injection, the bracket moves one step, the step is moved again, the first cleaning liquid injection needle can pump waste liquid away, the magnetic beads can be sucked to the inner side of the reaction cup by a magnet at the moment, the position of the liquid injection needle for pumping the waste liquid can be deviated to the outer side of the reaction cup, and the probability of sucking the magnetic beads away together can be reduced;
Then the bracket moves one step, the reaction cup is rotated to the position of a second cleaning liquid injection needle, the cleaning assembly moves to the bottom for injecting liquid, the bracket moves one step, the blending assembly below the reaction cup is opened at the moment, the liquid in the reaction cup is thoroughly mixed, the bracket moves one step, the second cleaning liquid extraction needle can extract the waste liquid, the magnetic beads can be absorbed to the inner side of the reaction cup by the magnet at the moment, the position of the liquid extraction needle extracting the waste liquid can be deviated to the outer side of the reaction cup, and the probability of absorbing the magnetic beads together can be reduced;
the bracket moves one step again, the reaction cup rotates to the third cleaning liquid injection needle position, the cleaning assembly moves to the bottom for injecting liquid, the bracket moves one step, the blending assembly below the reaction cup is opened at the moment, the liquid in the reaction cup is thoroughly mixed, the bracket moves one step, the third cleaning liquid extraction needle can extract the waste liquid, the magnetic beads can be absorbed to the inner side of the reaction cup by the magnet at the moment, the liquid extraction needle position for extracting the waste liquid can be deviated to the outer side of the reaction cup, and the probability of absorbing the magnetic beads together can be reduced.
Due to the adoption of the technical scheme, compared with the prior art, the invention has the advantages that: the invention discloses a device and a method for reducing loss of magnetic beads during magnetic separation, which can thoroughly clean the magnetic beads during magnetic liquid separation cleaning, can effectively reduce the loss of the magnetic beads during the cleaning process, have better effect and are suitable for popularization and application.
The invention is further described with reference to the following figures and detailed description.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a device for reducing the loss of magnetic beads during magnetic separation according to the present invention.
FIG. 2 is a schematic view of a partial structure of a device for reducing magnetic bead loss during magnetic separation according to the present invention.
FIG. 3 is a schematic view of a partial structure of a device for reducing magnetic bead loss during magnetic separation according to the present invention.
FIG. 4 is a schematic view of a partial structure of an apparatus for reducing magnetic bead loss during magnetic separation according to the present invention.
FIG. 5 is a schematic cross-sectional view of a partial structure of an apparatus for reducing magnetic bead loss during magnetic separation according to the present invention.
FIG. 6 is a schematic view of a partial structure of an apparatus for reducing magnetic bead loss during magnetic separation according to the present invention.
FIG. 7 is a schematic view of a partial structure of an apparatus for reducing magnetic bead loss during magnetic separation according to the present invention.
FIG. 8 is a schematic view of a partial structure of an apparatus for reducing magnetic bead loss during magnetic separation according to the present invention.
FIG. 9 is a schematic view of a partial structure of an apparatus for reducing magnetic bead loss during magnetic separation according to the present invention.
FIG. 10 is a schematic view of a partial structure of an apparatus for reducing magnetic bead loss during magnetic separation according to the present invention.
Detailed Description
Example (b): as shown in the attached drawings 1-10, a device for reducing magnetic bead loss during magnetic separation, including fixed reaction disc 1, reaction disc 1 level sets up, reaction disc 1 is circular, reaction disc 1 installs bracket 2 that can be at 1 internal rotation of reaction disc, bracket 2's shape cooperatees with reaction disc 1's shape, bracket 2 is last to radially evenly be provided with a plurality of mounting holes, the shape of mounting hole is the rectangle, install reaction cup 8 in the mounting hole, 1 periphery of reaction disc is provided with the driving tooth, driving tooth accessible gear, parts such as motor drive reaction disc 1 and rotate.
Fig. 4 to fig. 9 show, the mixing subassembly is including fixing the mixing motor support 64 at 1 lateral wall of reaction disc, mixing motor 61 is installed to mixing motor support 64, mixing seat support 67 is installed to 1 bottom of reaction disc, mixing seat support 67 level sets up, mixing seat support 67 is the arc, its radian is corresponding with reaction disc 1, mixing seat support 67 installs transmission shaft 69, mixing seat support 67 is exposed at the both ends of transmission shaft 69, the top fixed mounting of transmission shaft 69 has mixing head 68, mixing head 68 is corresponding with the position of reaction cup 8, synchronous pulley 65 is installed to the bottom of transmission shaft 69, mixing motor 61 is connected with synchronous pulley 65 transmission.
As shown in fig. 3 and 4, a driving wheel 62 is installed at the bottom of the blending motor 61, a driven wheel 63 is installed at the bottom of a blending motor support 64, one driven wheel 63 is provided, a transmission shaft 69 is installed on a blending base support 67 through two bearings, three transmission shafts 69 are provided, a three-way transmission shaft 69 is located on the same radian line, the radian line corresponds to the radian of the bracket 2, two transmission shafts 69 are located at one end of the blending base support 67, the other end of the blending base support 67 is located, three synchronous pulleys 65 are provided corresponding to the transmission shafts 69, a synchronous pulley 65 is installed on each transmission shaft 69, and a transmission belt 66 is installed on the driving wheel 62, the driven wheel 63 and the synchronous pulley 65.
As shown in fig. 5 and 9, the mixing head 68 includes an upper portion 681 and a lower portion 682, both the upper portion 681 and the lower portion 682 are cylindrical, the upper portion 681 and the lower portion 682 are eccentrically arranged, the lower portion 682 is coaxially arranged with the transmission shaft 69, a spherical groove 683 is arranged on the top surface of the upper portion 681, the center of the groove 683 coincides with the axis of the upper portion 681, and the bottom of the reaction cup 8 is located in the groove 683 when the reaction cup 8 corresponds to the mixing head 68.
As shown in fig. 2 and fig. 3, a fixing frame is fixedly arranged on the side wall of the reaction disk 1, a fixed support is arranged on the side portion of the device, the fixing frame is fixedly installed on the support, a driving motor 44 is installed at the bottom of the fixing frame, a movable plate 46 is installed in the fixing frame, the movable plate 46 is in transmission connection with the driving motor 44, and the movable plate 46 is provided with a cleaning assembly 5.
As shown in fig. 2, the fixing frame 4 includes a bottom plate 41, a top plate 43 and a side plate 42, the side plate 42 is fixedly connected with the fixing frame, the top plate 43 and the bottom plate 41 are disposed at two ends of the side plate 42 in parallel, the driving motor 44 is mounted at the bottom of the bottom plate 41, a sliding column 45 is disposed between the top plate 43 and the bottom plate 41, the sliding column 45 is slidably mounted with a movable plate 46, the movable plate 46 is provided with a threaded hole, the driving motor 44 is mounted with a screw 47 in a transmission manner, and the screw 47 passes through.
As shown in fig. 2, the cleaning assembly 5 includes a guide shaft 51 mounted on the movable plate 46, a cleaning carriage 52 is mounted on the top of the guide shaft 51, and a cleaning liquid injection needle 54 and a cleaning liquid extraction needle 53 are mounted on the cleaning carriage 52. The cleaning liquid injection needles 54 and the cleaning liquid extraction needles 53 are respectively provided with three, the positions of the cleaning liquid injection needles 54 and the cleaning liquid extraction needles 53 correspond to the positions of the bracket 2, and the three cleaning liquid injection needles 54 and the three cleaning liquid extraction needles 53 are arranged on the cleaning carriage 52 in a crossed manner.
As shown in the attached figure 1, the reaction disc 1 is provided with a light shield 3, and the light shield 3 corresponds to the position of the cleaning assembly. The light shield 3 is provided with a plurality of plate holes 31. The plate holes 32 correspond to the positions of the cleaning solution injection needle 54 and the cleaning solution suction needle 53, respectively.
A use method of the device for reducing magnetic bead loss during magnetic separation comprises the following steps that along with the anticlockwise rotation of a bracket 2 on a reaction disc 1, reaction cups 8 in the bracket 2 also rotate one by one, the position of a first cleaning liquid injection needle 54 is rotated, a cleaning assembly moves to bottom liquid injection, the lifting is carried out, the bracket 2 moves one step, the first cleaning liquid injection needle 53 can suck waste liquid, magnetic beads are sucked to the inner side of the reaction cups 8 by magnets at the moment, the position of the cleaning liquid injection needle 53 for sucking the waste liquid is also deviated to the outer side of the reaction cups 8, and the probability of sucking the magnetic beads together is reduced;
Then the bracket 2 moves one step, the reaction cup 8 is rotated to the position of a second cleaning and liquid injection needle 54, the cleaning assembly moves to the bottom for injecting liquid, the bottom is lifted, the bracket 2 moves one step, the blending assembly below the reaction cup 8 is opened at the moment, the liquid in the reaction cup 8 is thoroughly mixed, the bracket 2 moves one step, the second cleaning and liquid pumping needle 53 can pump the waste liquid away, the magnetic beads can be sucked to the inner side of the reaction cup 8 by the magnet at the moment, the position of the cleaning and liquid pumping needle 53 for pumping the waste liquid can be deviated to the outer side of the reaction cup 8, and the probability of sucking the magnetic beads away together can be reduced;
one step is walked again to bracket 2, reaction cup 8 changes the third piece and washs notes liquid needle 54 position, wash the subassembly and remove to the bottom and annotate the liquid, rise, bracket 2 walks one step, the mixing subassembly is opened below reaction cup 8 this moment, thoroughly mix the liquid in reaction cup 8, bracket 2 walks one step, third piece is washd and is taken out liquid needle 53 and can be taken out the waste liquid, the magnetic bead can be inhaled reaction cup 8 inboard by magnet this moment, the washing of taking out the waste liquid is taken out liquid needle 53 position and also can be partial to the reaction cup 8 outside, will reduce the probability of taking out the magnetic bead together.
The magnetic-liquid separation of the device adopts 3 short needles as cleaning liquid injection needles and 3 long needles as cleaning liquid extraction needles, each reaction cup can be cleaned for 3 times, and the adoption of a separation structure can effectively avoid inaccurate experimental results caused by pollution carried by the cleaning needles;
The 3 short needles are arranged at the positions of the cleaning liquid injection needles 54 and are deviated to the inner side of the center of the reaction disc 1, and the 3 long needles are arranged at the positions of the cleaning liquid extraction needles and are deviated to the outer side of the center of the reaction disc;
the reaction cup 8 is placed on a bracket, and the bracket 2 is placed on the reaction disk 1;
a magnet bracket made of steel is arranged at the cleaning position on the reaction disc 1, a magnet 71 is arranged on the magnet bracket 7 and can be adsorbed at the inner side of the reaction cup 8 by magnetic beads, and when the cleaning liquid-extracting needle 53 extracts waste liquid, the waste liquid can be pricked to the outer side of the reaction cup 8 to extract the waste liquid without extracting the magnetic beads;
a mixing component is arranged at the bottom of the reaction cup 8 at the cleaning position, reactants coated with magnetic beads and reactants not coated with magnetic beads are thoroughly mixed, then the reactants coated with the magnetic beads are adsorbed on the inner wall of the reaction cup 8 under the action of an internal magnet, and finally the cleaning waste liquid is sucked away;
the mode that 3 wash draw liquid needle 53 extraction waste liquid is the peristaltic pump extraction, can replace the mode of vacuum cup in the past with the diaphragm pump, and the benefit is speed, pressure, three needle uniformity when the waste liquid of extraction all can guarantee, has practiced thrift the cost simultaneously, has reduced the magnetic loss, has more increased the stability of illuminating apparatus result.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. The utility model provides a device of magnetic bead loss when reducing magnetic separation, includes reaction disc, its characterized in that: the reaction disc is installed and can be at the bracket of reaction disc internal rotation, install reaction cup on the bracket, magnet support is installed to the reaction disc, magnet is installed to magnet support, reaction cup and magnet support position are corresponding, and when reaction cup moved the position of magnet support through the bracket, reaction cup was located the magnetic force district of magnet, the mixing subassembly is installed to the reaction disc bottom, the mixing subassembly is corresponding with the reaction cup position of bracket installation.
2. A device for reducing the loss of magnetic beads during magnetic separation as claimed in claim 1, wherein: the mixing subassembly is including fixing the mixing motor support at the reaction disc lateral wall, mixing motor support mounting has the mixing motor, mixing seat support is installed to the reaction disc bottom, mixing seat support mounting has the transmission shaft, the mixing head is installed to the one end of transmission shaft, the mixing head is corresponding with the position of reaction cup, the mixing motor is connected with the transmission shaft transmission.
3. A device for reducing the loss of magnetic beads during magnetic separation as claimed in claim 2, wherein: the mixing motor is installed the drive wheel bottom, mixing motor support bottom is installed from the driving wheel, the one end that the mixing head was kept away from to the transmission shaft is installed synchronous pulley, install the drive belt on drive wheel, the follow driving wheel and the synchronous pulley.
4. A device for reducing the loss of magnetic beads during magnetic separation as claimed in claim 2, wherein: the mixing head comprises an upper part and a lower part, the upper part and the lower part are eccentrically arranged, a spherical groove is formed in the top surface of the upper part, and the bottom of the reaction cup is positioned in the groove when the reaction cup corresponds to the mixing head.
5. A device for reducing the loss of magnetic beads during magnetic separation as claimed in any one of claims 1 to 4, wherein: the reaction disc side wall is fixedly provided with a fixing frame, a driving motor is installed at the bottom of the fixing frame, a movable plate is installed in the fixing frame and is in transmission connection with the driving motor, and a cleaning assembly is installed on the movable plate.
6. An apparatus as claimed in claim 5, wherein the magnetic beads are magnetically separated from the sample by: the fixing frame comprises a bottom plate, a top plate and side plates, the side plates are fixedly connected with the fixing frame, the top plate and the bottom plate are arranged at two ends of each side plate in parallel, the driving motor is installed at the bottom of the bottom plate, a sliding column is arranged between the top plate and the bottom plate, a movable plate is installed on the sliding column in a sliding mode, a threaded hole is formed in the movable plate, a first motor is provided with a screw rod, and the screw rod penetrates through the threaded hole to be in threaded connection with.
7. An apparatus as claimed in claim 5, wherein the magnetic beads are magnetically separated from the sample by: the cleaning assembly comprises a guide shaft arranged on the movable plate, a cleaning carriage is arranged at the top of the guide shaft, and a cleaning liquid injection needle and a cleaning liquid extraction needle are arranged on the cleaning carriage.
8. An apparatus as claimed in claim 7, wherein the magnetic beads are magnetically separated from the sample by: the cleaning device is characterized in that the cleaning injection needles and the cleaning drawing needles are respectively provided with three needles, the positions of the cleaning injection needles and the cleaning drawing needles correspond to the positions of the bracket, and the three cleaning injection needles and the three cleaning drawing needles are arranged on the cleaning carriage in a crossed manner.
9. A device for reducing the loss of magnetic beads during magnetic separation as claimed in any one of claims 1 to 4, wherein: the reaction disc is provided with a light shield, and the light shield corresponds to the cleaning assembly in position.
10. A method for using the device for reducing the loss of magnetic beads during magnetic separation according to claim 1, wherein: along with the anticlockwise rotation of a bracket on the reaction disc, reaction cups in the bracket can also rotate one by one, the reaction cups are rotated to the position of a first cleaning liquid injection needle, a cleaning assembly moves to the bottom for injecting liquid, the cleaning assembly is lifted, the bracket moves one step and then moves one step, the first cleaning liquid extraction needle can extract waste liquid, magnetic beads can be absorbed to the inner side of the reaction cup by a magnet, the position of the liquid extraction needle for extracting the waste liquid can also deviate to the outer side of the reaction cup, and the probability of absorbing the magnetic beads together can be reduced;
Then the bracket moves one step, the reaction cup is rotated to the position of a second cleaning liquid injection needle, the cleaning assembly moves to the bottom for injecting liquid, the bracket moves one step, the blending assembly below the reaction cup is opened at the moment, the liquid in the reaction cup is thoroughly mixed, the bracket moves one step, the second cleaning liquid extraction needle can extract the waste liquid, the magnetic beads can be absorbed to the inner side of the reaction cup by the magnet at the moment, the position of the liquid extraction needle extracting the waste liquid can be deviated to the outer side of the reaction cup, and the probability of absorbing the magnetic beads together can be reduced;
the bracket moves one step again, the reaction cup rotates to the third cleaning liquid injection needle position, the cleaning assembly moves to the bottom for injecting liquid, the bracket moves one step, the blending assembly below the reaction cup is opened at the moment, the liquid in the reaction cup is thoroughly mixed, the bracket moves one step, the third cleaning liquid extraction needle can extract the waste liquid, the magnetic beads can be absorbed to the inner side of the reaction cup by the magnet at the moment, the liquid extraction needle position for extracting the waste liquid can be deviated to the outer side of the reaction cup, and the probability of absorbing the magnetic beads together can be reduced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910947732.4A CN111841877A (en) | 2019-10-08 | 2019-10-08 | Device for reducing magnetic bead loss in magnetic separation and use method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910947732.4A CN111841877A (en) | 2019-10-08 | 2019-10-08 | Device for reducing magnetic bead loss in magnetic separation and use method |
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| CN111841877A true CN111841877A (en) | 2020-10-30 |
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| CN201910947732.4A Pending CN111841877A (en) | 2019-10-08 | 2019-10-08 | Device for reducing magnetic bead loss in magnetic separation and use method |
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| CN113020097A (en) * | 2021-01-29 | 2021-06-25 | 上海市浦东新区周浦医院 | Rotatable magnetic bead cleaning system |
| CN113275342A (en) * | 2021-06-22 | 2021-08-20 | 宙斯生命科技(常州)有限公司 | Reaction cup washing mechanism and reaction cup washing method |
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| CN113020097A (en) * | 2021-01-29 | 2021-06-25 | 上海市浦东新区周浦医院 | Rotatable magnetic bead cleaning system |
| CN113020097B (en) * | 2021-01-29 | 2023-09-08 | 上海市浦东新区周浦医院 | Rotatable magnetic bead cleaning system |
| CN113275342A (en) * | 2021-06-22 | 2021-08-20 | 宙斯生命科技(常州)有限公司 | Reaction cup washing mechanism and reaction cup washing method |
| CN113275342B (en) * | 2021-06-22 | 2023-11-14 | 宙斯生命科技(常州)有限公司 | Reaction cup washing mechanism and reaction cup washing method |
| CN115156215A (en) * | 2022-08-02 | 2022-10-11 | 宋世琦 | Magnetic particle cleaning device and cleaning method for immunoassay device |
| CN115888507A (en) * | 2023-01-05 | 2023-04-04 | 山东康华生物医疗科技股份有限公司 | Ultrasonic mixing device for reaction cup |
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