CN113862149B - Shimmy integrated cell mechanics loading device - Google Patents
Shimmy integrated cell mechanics loading device Download PDFInfo
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- CN113862149B CN113862149B CN202111075896.6A CN202111075896A CN113862149B CN 113862149 B CN113862149 B CN 113862149B CN 202111075896 A CN202111075896 A CN 202111075896A CN 113862149 B CN113862149 B CN 113862149B
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- 238000004113 cell culture Methods 0.000 claims abstract description 85
- 230000007246 mechanism Effects 0.000 claims abstract description 54
- 210000004027 cell Anatomy 0.000 claims description 17
- 210000004748 cultured cell Anatomy 0.000 claims description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 5
- 230000000638 stimulation Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 210000004081 cilia Anatomy 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000035992 intercellular communication Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Mechanical Engineering (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a shimmy integrated cell mechanics loading device which integrates swinging and vibration and can provide accurate loading data for a cell culture dish. The loading device comprises: the vibration support base, the box body, the vibration driving mechanism, the dish carrying mechanism and the rotation driving mechanism; the supporting base is used for supporting the box body and the rotary driving mechanism; the dish carrying mechanism is used for placing the cell culture dish and is arranged in the box body; the vibration driving mechanism is used for driving the dish carrying mechanism to vibrate up and down in the box body along the direction vertical to the bottom surface of the box body; the rotary driving mechanism is directly connected with the box body and is used for driving the box body to swing left and right.
Description
Technical Field
The invention relates to a loading device, in particular to a cell mechanics loading device, and belongs to the technical field of cell mechanics culture.
Background
Sources of motion and stimulation that affect embryo development include shear stress from tubal fluid flow, compression from peristaltic tubal wall motion, buoyancy between embryo and cilia, and kinetic friction. Mechanical stimulation in vivo is thought to induce intercellular communication, exert beneficial effects by accelerating fluid surrounding the embryo, and digest metabolites expelled from the embryo. Microvibration stimulation during the culture of mouse embryos has a positive effect on the development rate. The microvibration embryo culture method simulating in-vivo mechanical force is an effective way for improving the quality of in-vitro fertilization treatment.
The current approach is to tilt machines and micro-vibrators to simulate mechanical stimulation in the body. The tilting machine is used for placing the cell culture dish on the rotating shaft, the rotating shaft is connected with the motor, and the motor drives the culture dish to periodically swing at a certain angle and frequency, so that the effect of simulating the shearing force of cell fluid is achieved. The miniature vibrator vibrates by driving the vibration spring, so as to simulate the vibration force suffered by embryo cells.
The prior stem cell culture device mainly has the following defects:
(1) The requirements of vibration and swing cannot be met at the same time; and because the direction of the vibration is required to be perpendicular to the rotation time, the vibration and the swing are assembled by adopting independent structures, so that errors can be brought, and the accuracy of loading data can be influenced.
(2) The whole device is put into a cell incubator for working, and the traditional vibrating instrument is generally large in size and high in price.
(3) The independent rotating structure and the vibrating structure are assembled with each other, so that the problems of energy loss and friction errors are caused, and the loading data are inaccurate.
Disclosure of Invention
The purpose of the invention is that: aiming at the defects of the prior art, the device provides a shimmy integrated cell mechanics loading device which integrates swinging and vibration and can provide accurate loading data for a cell culture dish.
The technical scheme of the invention is as follows: a shimmy integrated cell mechanical loading device comprising: the device comprises a supporting base, a box body, a vibration driving mechanism, a dish carrying mechanism and a rotation driving mechanism;
the supporting base is used for supporting the box body and the rotary driving mechanism;
the dish carrying mechanism is positioned in the box body and is used for placing a cell culture dish;
the vibration driving mechanism is used for driving the carrier mechanism to vibrate up and down in the box body along the direction vertical to the bottom surface of the box body;
the rotary driving mechanism is directly connected with the box body and is used for driving the box body and the dish carrying mechanism to swing left and right integrally.
As a preferred embodiment of the present invention, the vibration drive mechanism includes: the rotating motor B, the connecting rod and the turntable;
the rotary motor B is fixed in the box body, the power output end of the rotary motor B is connected with the rotary table, and the rotary table is connected with the dish carrying mechanism through a connecting rod;
the connecting hole of connecting rod on the carousel is eccentric hole, works as rotating electrical machines B drives the carousel rotates around its self axis, the carousel passes through the connecting rod and drives the dish mechanism reciprocates in order to realize the vibration in the box.
As a preferable mode of the invention, the inner side surface of the box body is provided with a sliding rail; the dish carrying mechanism is in sliding fit with the sliding rail through the idler wheels, and when the dish carrying mechanism vibrates up and down in the box body, the pulley slides on the sliding rail on the inner side of the box body.
As a preferred embodiment of the present invention, the dish carrying mechanism includes: cell culture dish carrier plate and cell culture dish;
placing more than one cell culture dish on the cell culture dish carrier plate;
the cell culture dish is used for placing and culturing cells;
the vibration driving mechanism drives the cell culture dish carrier plate to vibrate up and down in the box body.
As a preferred mode of the invention, each cell culture dish is covered with a cell culture dish cover plate; the cell culture dish cover plate is fixed on the cell culture dish carrier plate.
As a preferable mode of the invention, more than two culture dish baffles are distributed on the outer circumference of each culture dish groove on the cell culture dish carrier plate, and after the culture dish baffles position the cell culture dish, the cell culture dish cover plate is fixedly connected with the culture dish baffles through a knock pin.
As a preferred embodiment of the present invention, the rotation driving mechanism includes: a rotating electric machine A; one side of the two opposite sides of the box body is connected with the power output end of the rotating motor A, the other end of the box body is supported on the box body through a bearing, and the box body is driven to swing wholly and reciprocally through the reciprocating rotation of the rotating motor A.
As a preferred mode of the invention, the cell culture dish carrier plate is provided with more than two sectional culture dish tanks, and one cell culture dish is placed above each culture dish tank.
As a preferred mode of the invention, the culture dish tank is a through tank.
As a preferable mode of the invention, the amplitude of the dish carrying mechanism is adjusted by adjusting the distance between the connecting hole of the connecting rod on the turntable and the circle center of the turntable.
The beneficial effects are that:
(1) This loading device is with vibration and swing function integration, reduces the error that independent structure assembly brought, drives the slide up and down of culture dish carrier plate through the carousel and reaches vibration function, and the slip direction of cell culture dish carrier plate is perpendicular to box bottom surface all the time during the vibration, has guaranteed that the vibration direction that the culture dish received in rotatory (swing promptly) in-process is perpendicular with rotation direction all the time.
(2) The functions are adjustable: the loading device can enable the cell culture dish to have three modes of load loading: rotation, vibration, and rotation plus vibration.
(3) The adjustable valve is provided with the following adjustability: the vibration device on the market at present has the advantages that the vibration amplitude cannot be controlled in a small size, the size of the large size is too large, the vibration amplitude of the cell culture dish carrier plate can be adjusted only by adjusting the distance from the connecting part of the connecting rod and the turntable to the circle center of the turntable, the vibration amplitude can be measured, and the vibration amplitude can be accurately measured through the position distance between the turntable and the connecting rod.
(4) The cost is low: the form that the rotary disc drives the cell culture dish carrier plate to slide back and forth is adopted to replace a complex vibration amplitude adjustable vibration structure, so that the cost is greatly saved.
Drawings
FIG. 1 is an exploded view of a cell mechanical loading device according to the present invention;
FIG. 2 is an assembly schematic diagram of the cell mechanical loading device of the present invention.
In the figure: 1-a supporting base; 2-rotating electric machine a; 3-a box body; 4-ejector pins; 5-cell culture dish carrier plate; 6-cell culture dish cover plate; 7-cell culture dishes; 8-a roller; 9-connecting rods; 10-rotating disk.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Based on the mechanical environment where the embryonic cells are located, the embodiment provides a shimmy integrated cell mechanical loading device which is used for researching the development quality of the embryonic stem cells under mechanical regulation, integrates the swinging and the vibration, and has accurate and measurable amplitude.
As shown in fig. 1 and 2, the cytomechanical loading device includes: support base 1, box 3, vibration drive mechanism, load dish mechanism and rotary drive mechanism.
Wherein the carrier mechanism includes: a cell culture dish carrier plate 5, a cell culture dish cover plate 6 and a cell culture dish 7; the box body 3 is a cuboid with an opening at the top, and sliding rails are arranged on four inner side surfaces of the box body 3; the four sides of the cell culture dish carrier plate 5 are respectively provided with a roller 8 in sliding fit with the sliding rail, so that the up-and-down sliding of the cell culture dish carrier plate 5 in the box body 3 is guided by the sliding fit of the rollers 8 and the sliding rail, and meanwhile friction is reduced; the vertical sliding of the cell culture dish carrier plate 5 in the box body 3 realizes the vibration in the direction vertical to the bottom surface of the box body 3.
The cell culture dish carrier plate 5 is used for placing a cell culture dish 7, and four sectional culture dish grooves are formed in the cell culture dish carrier plate 5 (namely, the culture dish grooves are respectively and independently arranged, and the four culture dish grooves are distributed on the cell culture dish carrier plate 5 in a rectangular shape); above the culture dish tank is placed a cell culture dish 7 (i.e. the diameter of the cell culture dish 7 is larger than the diameter of the culture dish tank). The culture dish groove adopts four sections, which is beneficial to ventilating the cell culture dish 7; the culture dish groove is a through groove, namely the surface of the culture dish groove is hollowed out, so that the cell culture condition can be observed from the inside of the box body 3. The middle parts of the four sides of the cell culture dish carrier plate 5 are connected with rollers 8.
The cell culture dish 7 is used for placing cultured cells; to prevent the cell culture dishes 7 from being separated from the cell culture dish carrier plate 5 during vibration, a cell culture dish cover plate 6 is covered on each cell culture dish 7, and the cell culture dish cover plates 6 are fixed on the cell culture dish carrier plate 5 through the ejector pins 4. Specific: four culture dish baffles are uniformly distributed at intervals on the outer circumference of each culture dish groove on the cell culture dish carrier plate 5, threaded holes are formed in each culture dish baffle, and after the cell culture dish 7 is positioned by the culture dish baffles, the cell culture dish cover plate 6 and the culture dish baffle are fixedly connected on the cell culture dish 7 through the ejector pins 4.
The rotary driving mechanism is used for driving the box body 3 to reciprocally rotate (namely, driving the box body to swing left and right). The rotary driving mechanism adopts a rotary motor A2; the front and rear sides of the box body 3 are respectively connected with the rotating motor A2 and the supporting base 1, and therefore the box body 3 is driven to integrally and reciprocally rotate through the reciprocal rotation of the rotating motor A2. The method comprises the following steps: a hexagonal long groove for connecting with a connecting sleeve is formed in the middle of a front plate of the box body 3, one end of the connecting sleeve is connected with the box body 3 through a hexagonal bolt, the other end of the connecting sleeve is connected with a power output end of the rotating motor A2, and a rear plate of the box body 3 is supported on the supporting base 1 through a bearing; therefore, when the rotating motor A2 rotates, the whole box body 3 can be driven to rotate around the axis of the rotating motor A2, namely, the left-right swing of the box body 3 is realized, and further, the left-right swing of the dish carrying mechanism in the box body 3 is realized.
The support base 1 is used for supporting the rotating motor A2 and the box 3; one side of the supporting base 1 is in a groove shape and is used for placing the rotating motor A; the other side is used for supporting the box body 3.
The vibration driving mechanism includes: a rotating motor B, a connecting rod 9 and a turntable 10; wherein rotating electrical machines B fix inside box 3, and rotating electrical machines B's power take off end links to each other with carousel 10, and carousel 10 passes through connecting rod 9 and links to each other with cell culture dish carrier plate 5. The connecting position of the connecting rod 9 and the turntable 10 is the non-center position of the turntable 10, namely the connecting hole of the connecting rod 9 on the turntable 10 is an eccentric hole. Therefore, when the rotating motor B drives the rotary table 10 to rotate, the rotary table 10 can drive the cell culture dish carrier plate 5 to slide up and down through the connecting rod 9 so as to achieve the effect of vibration. The sliding displacement of the cell culture dish carrier plate 5 is twice the distance from the connecting part of the rotary disk 10 and the connecting rod 9 to the circle center of the rotary disk 10. When the cell culture dish carrier plate 5 slides up and down, the pulley 8 on the cell culture dish carrier plate 5 slides on the sliding rail on the inner side of the box body, and the effect of reducing sliding friction force can be achieved.
The loading device can load the cell culture dish 7 with three modes of load: (1) single rotation; (2) single vibration; (3) rotation plus vibration; the specific steps of loading the cell culture dish 7 by the loading device are as follows:
firstly, placing a cell culture dish 7 in a culture dish groove of a rotatable carrying dish device 5; the cell culture dish 7 is then covered with a cell culture dish cover plate 6, and the cell culture dish cover plate 6 is fixed by the ejector pins 4.
Single rotation loading mode: and the rotating motor A2 is started, and the rotating motor A2 drives the box body 3 to rotate, so that the whole left-right swing of the dish carrying mechanism and the rotary driving mechanism is realized.
Single vibration loading mode: the rotating motor B is started, the turntable 10 rotates, and then the cell culture dish carrier plate 5 is driven to slide up and down along the sliding rail on the box body 3 through the connecting rod 9 so as to realize vibration loading on the cell culture dish 7.
Rotation plus vibration loading mode: rotating electric machine A2 and rotating electric machine B are started at the same time.
The amplitude of the cell culture dish carrier plate 5 can be adjusted by adjusting the distance from the connecting position of the connecting rod 9 on the turntable 10 to the circle center of the turntable 10, namely, the connecting position of the connecting rod 9 and the turntable 10.
While the invention has been described in detail in the foregoing general description and specific examples, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.
Claims (7)
1. Shimmy integrated cell mechanics loading device, its characterized in that includes: the device comprises a supporting base (1), a box body (3), a vibration driving mechanism, a dish carrying mechanism and a rotation driving mechanism;
the supporting base (1) is used for supporting the box body (3) and the rotary driving mechanism;
the dish carrying mechanism is positioned in the box body (3) and is used for placing a cell culture dish (7);
the vibration driving mechanism is used for driving the carrier mechanism to vibrate up and down in the box body (3) along the direction vertical to the bottom surface of the box body (3);
the rotary driving mechanism is directly connected with the box body (3) and is used for driving the box body (3) and the dish carrying mechanism to swing left and right integrally;
the vibration driving mechanism includes: a rotating motor B, a connecting rod (9) and a turntable (10);
the rotary motor B is fixed inside the box body (3), the power output end of the rotary motor B is connected with the rotary table (10), and the rotary table (10) is connected with the dish carrying mechanism through a connecting rod (9);
the connecting hole of the connecting rod (9) on the rotary table (10) is an eccentric hole, and when the rotary motor B drives the rotary table (10) to rotate around the axis of the rotary table, the rotary table (10) drives the dish carrying mechanism to move up and down in the box body (3) through the connecting rod (9) so as to realize vibration; the amplitude of the dish carrying mechanism is adjusted by adjusting the distance from the connecting hole of the connecting rod (9) on the turntable (10) to the circle center of the turntable (10);
the rotation driving mechanism includes: a rotating electrical machine A (2); one side of two opposite sides of the box body (3) is connected with the power output end of the rotating motor A (2), the other end of the box body is supported on the box body (3) through a bearing, and the box body (3) is driven to swing in a whole reciprocating manner through the reciprocating rotation of the rotating motor A (2);
the loading device loads the cell culture dish (7) with three modes of loading: single rotation, single vibration, rotation plus vibration.
2. The shimmy integrated cell mechanics loading device according to claim 1, wherein a sliding rail is arranged on the inner side surface of the box body (3); the dish carrying mechanism is in sliding fit with the sliding rail through the roller (8), and when the dish carrying mechanism vibrates up and down in the box body (3), the roller (8) slides on the sliding rail on the inner side of the box body (3).
3. The shimmy integrated cytomechanical loading device of claim 1, wherein the dish mechanism comprises: a cell culture dish carrier plate (5) and a cell culture dish (7);
more than one cell culture dish (7) is placed on the cell culture dish carrier plate (5);
the cell culture dish (7) is used for placing cultured cells;
the vibration driving mechanism drives the cell culture dish carrier plate (5) to vibrate up and down in the box body (3).
4. A shimmy integrated cell mechanical loading device according to claim 3, characterized in that each of said cell culture dishes (7) is covered with a cell culture dish cover plate (6); the cell culture dish cover plate (6) is fixed on the cell culture dish carrier plate (5).
5. The shimmy integrated cell mechanical loading device according to claim 4, wherein more than two culture dish baffles are distributed on the outer circumference of each culture dish groove on the cell culture dish carrier plate (5), and after the culture dish baffles position the cell culture dish (7), the cell culture dish cover plate (6) is fixedly connected with the culture dish baffles through the ejector pins (4).
6. A shimmy integrated cell mechanical loading device according to claim 3, characterized in that more than two sectional culture dish tanks are arranged on the cell culture dish carrier plate (5), and a cell culture dish (7) is arranged above each culture dish tank.
7. The shimmy integrated cytomechanical loading device of claim 6, wherein the culture dish is a through-groove.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111075896.6A CN113862149B (en) | 2021-09-14 | 2021-09-14 | Shimmy integrated cell mechanics loading device |
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| CN202111075896.6A CN113862149B (en) | 2021-09-14 | 2021-09-14 | Shimmy integrated cell mechanics loading device |
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| CN113862149A CN113862149A (en) | 2021-12-31 |
| CN113862149B true CN113862149B (en) | 2023-10-27 |
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| CN114231482B (en) * | 2021-11-09 | 2023-07-14 | 北京理工大学 | Method and vibration device for forming parthenogenetic blastocyst by continuous vibration stimulation of human immature oocyte |
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