CN110684660A - Micro-vibration culture device for embryos - Google Patents
Micro-vibration culture device for embryos Download PDFInfo
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- CN110684660A CN110684660A CN201911060778.0A CN201911060778A CN110684660A CN 110684660 A CN110684660 A CN 110684660A CN 201911060778 A CN201911060778 A CN 201911060778A CN 110684660 A CN110684660 A CN 110684660A
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- 210000002257 embryonic structure Anatomy 0.000 title claims abstract description 18
- 230000033001 locomotion Effects 0.000 claims abstract description 38
- 230000005540 biological transmission Effects 0.000 claims description 19
- 238000012258 culturing Methods 0.000 claims description 9
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 31
- 238000011161 development Methods 0.000 abstract description 11
- 230000018109 developmental process Effects 0.000 abstract description 11
- 230000002503 metabolic effect Effects 0.000 abstract description 9
- 239000002699 waste material Substances 0.000 abstract description 9
- 235000015097 nutrients Nutrition 0.000 abstract description 7
- 210000004291 uterus Anatomy 0.000 abstract description 5
- 230000013020 embryo development Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 6
- 230000001276 controlling effect Effects 0.000 description 5
- 238000012136 culture method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention relates to the technical field of biomedicine, in particular to a micro-vibration culture device for embryos. It comprises a base, a motion platform and a rotating disk; a first driving mechanism for driving the moving table to do linear reciprocating motion on the base is arranged in the base; a second driving mechanism for driving the rotating disc to rotate on the moving table is arranged in the moving table; the rotating disc is used for placing a culture dish; the base is provided with a control panel, and the first driving mechanism and the second driving mechanism are controlled by the control panel. The invention adjusts the linear repeated movement speed of the moving platform and the rotating speed of the rotating disc through the control panel; so that the culture dish on the rotating disc obtains a micro-vibration culture environment; thereby reducing the concentration of metabolic waste around the embryo and ensuring that the periphery of the embryo has continuous and stable nutrient concentration so as to promote the development of the embryo; meanwhile, the environment that the embryo moves in the uterus is fully simulated, and the most appropriate micro-vibration culture environment is found, so that the embryo development is facilitated.
Description
Technical Field
The invention relates to the technical field of biomedicine, in particular to a micro-vibration culture device for embryos.
Background
In recent years, more and more infertility patients have own children by the aid of assisted reproduction technology, and the method mainly comprises the steps of combining sperms and ova of the patients in vitro and developing the sperms and the ova into embryos in a constant-temperature constant-humidity three-gas incubator.
Currently, the embryo culture method is conventionally performed by a "static culture method" in which a culture dish containing an embryo is left in an incubator. The research finds that: the embryo can secrete some metabolic waste in the process of culturing, because the embryo culturing environment is always in a static state, the metabolic waste is difficult to disperse in a short period, the development of the embryo is possibly influenced along with the increase of the content of the metabolic waste around the embryo, and meanwhile, the environment of the embryo culturing also simulates the environment of the uterus of a human body, so that the embryo has constant temperature, humidity and lower oxygen partial pressure,
therefore, there is a need to develop a device for culturing embryos by micro-vibration, so as to utilize the micro-vibration to diffuse metabolic wastes in time and sufficiently simulate the environment in which the embryos move in the uterus, thereby facilitating the development of the embryos.
Disclosure of Invention
In view of the above-mentioned shortcomings of the prior art, the present invention is directed to a micro-vibration embryo culture device.
In order to achieve the purpose, the invention adopts the following technical scheme:
a micro-vibration culture device for embryos comprises a base, a motion platform connected to the base in a sliding manner and a rotating disk connected to the motion platform in a rotating manner; a first driving mechanism for driving the moving table to do linear reciprocating motion on the base is arranged in the base; a second driving mechanism for driving the rotating disc to rotate on the moving table is arranged in the moving table; the rotating disc is used for placing a culture dish; the base is further provided with a control panel, and the first driving mechanism and the second driving mechanism are controlled by the control panel.
Preferably, the first driving mechanism comprises a first motor, a rotating rod, a connecting rod and a linkage shaft; the first motor is fixedly arranged in the base; the linkage shaft is fixedly arranged at the bottom of the motion platform and is distributed in parallel relative to the output shaft of the first motor; the output shaft of the first motor is in transmission connection with the linkage shaft sequentially through a rotary rod and a connecting rod, the rotary rod is distributed along the radial direction of the output shaft of the first motor, one end of the rotary rod is fixedly connected to the output shaft of the first motor, and the other end of the rotary rod is hinged to one end of the connecting rod; the connecting rods are distributed perpendicular to the linkage shaft, and the other ends of the connecting rods are hinged with the linkage shaft;
the base is provided with one or more guide rails, the bottom of the moving platform is provided with a sliding block matched with the guide rails, and the first motor is controlled by the control panel.
Preferably, the second driving mechanism comprises a second motor, a driving gear and one or more driven gears; the second motor is fixedly arranged in the motion table; the driving gear is sleeved on an output shaft of the second motor, the driven gears are rotatably connected with the motion platform, and the driving gear is in meshing transmission connection with each driven gear; and a rotating disk is coaxially and fixedly arranged at the top of each driven gear, and the second motor is controlled by a control panel.
Preferably, the motion table comprises a frame body and a shell covering the outer side of the frame body, wherein the frame body comprises a bottom plate, a top plate and a plurality of fixing shafts arranged between the bottom plate and the top plate; the second motor is fixedly arranged at the bottom of the top plate, an output shaft of the second motor penetrates through the top plate to be connected with the driving gear, and the driven gears are rotatably connected to the top plate through a rotating shaft; the driving gear and the driven gear are both packaged in the shell, and the rotating disc is exposed out of the shell and distributed.
Preferably, the number of the driven gears is four, and the four driven gears are uniformly distributed around the driving gear.
Preferably, one or more transmission gears are further arranged between the driving gear and the driven gear, and the driving gear is in meshed transmission connection with the driven gear through the transmission gears.
Preferably, a containing groove for placing the culture dish is formed in the center of the top surface of the rotating disc, and two notch portions are symmetrically distributed on the periphery of the containing groove.
Preferably, the bottom of the base is provided with a soft rubber cushion.
Due to the adoption of the scheme, the invention sets and adjusts the motion mode and parameters through the control panel; specifically, the speed of the linear repeated motion of the motion platform is adjusted by adjusting and controlling the first driving mechanism; regulating the rotation speed of the rotating disc by regulating and controlling the second driving mechanism; the rotating disc is arranged on the moving platform, so that the rotating disc can do reciprocating motion in the horizontal direction along with the moving platform, a culture dish on the rotating disc can have a complex motion track, embryo solutions in the culture dish can be uniformly mixed, and a micro-vibration culture environment is obtained; on one hand, metabolic waste is diffused in time by utilizing a micro-vibration movement mode, the concentration of the metabolic waste around the embryo is reduced, the influence on the development of the embryo caused by the fact that the embryo needs to absorb nutrient substances due to the development and the nutrient substances which are lacking around the embryo are avoided, and the continuous and stable nutrient substance concentration around the embryo is ensured in the development process of the embryo so as to promote the development of the embryo; on the other hand, the experiment can be carried out through the parameter adjustment of the control panel, the environment that the embryo is in motion in the uterus is fully simulated, the most appropriate micro-vibration culture environment is found, and the embryo development is more facilitated.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is an exploded view of an embodiment of the present invention;
FIG. 3 is a schematic structural view of a first drive mechanism of an embodiment of the present invention;
FIG. 4 is a schematic diagram of a motion stage according to an embodiment of the present invention;
FIG. 5 is a cross-sectional view of the interior of a motion stage according to an embodiment of the present invention;
FIG. 6 is a schematic structural view of a rotary disk according to an embodiment of the present invention;
fig. 7 is a schematic cross-sectional structure of an embodiment of the present invention.
Detailed Description
The embodiments of the invention will be described in detail below with reference to the drawings, but the invention can be implemented in many different ways as defined and covered by the claims.
As shown in fig. 1 to 7, the present invention provides an apparatus for micro-vibration culturing of embryos, which comprises a base 10, a moving platform 20 slidably connected to the base 10, and a rotating disc 30 rotatably connected to the moving platform 20; a first driving mechanism 40 for driving the moving table 20 to linearly and repeatedly move on the base 10 is arranged in the base 10; a second driving mechanism 50 for driving the rotating disc 30 to rotate on the moving table 20 is arranged in the moving table 20; the rotating disc 30 is used for placing a culture dish; the base 10 is further provided with a control panel 11, and the first driving mechanism 40 and the second driving mechanism 50 are controlled by the control panel 11. Thus, the movement pattern and parameters can be set and adjusted by the control panel 11; specifically, the speed of the linear repetitive movement of the moving stage 20 is adjusted by adjusting and controlling the first driving mechanism 40; the rotation speed of the rotating disk 30 is adjusted by adjusting and controlling the second driving mechanism 50; because the rotating disc 30 is arranged on the moving platform 20, the rotating disc 30 can reciprocate horizontally along with the moving platform 20, so that the culture dish on the rotating disc 30 can have a complex moving track, embryo solutions in the culture dish can be uniformly mixed, and a micro-vibration culture environment is obtained; on one hand, metabolic waste is diffused in time by utilizing a micro-vibration movement mode, the concentration of the metabolic waste around the embryo is reduced, the influence on the development of the embryo caused by the fact that the embryo needs to absorb nutrient substances due to the development and the nutrient substances which are lacking around the embryo are avoided, and the continuous and stable nutrient substance concentration around the embryo is ensured in the development process of the embryo so as to promote the development of the embryo; on the other hand, the experiment can be carried out through the parameter adjustment of the control panel 11, the environment that the embryo is in motion in the uterus is fully simulated, the most suitable micro-vibration culture environment is found, and the embryo development is more facilitated.
As a specific embodiment of the micro-vibration culture apparatus provided in this embodiment, referring to fig. 2 and 3, the first driving mechanism 40 includes a first motor 41, a rotating rod 42, a connecting rod 43, and a linkage shaft 44; the first motor 41 is fixedly arranged in the base 10, and the first motor 41 can be suspended in the base 10 through a fixing frame 45; the linkage shaft 44 is fixedly arranged at the bottom of the motion table 20, and the linkage shaft 44 and the output shaft of the first motor 41 are distributed in parallel relatively; an output shaft of the first motor 41 is in transmission connection with a linkage shaft 44 sequentially through a rotating rod 42 and a connecting rod 43, the rotating rod 42 is distributed along the radial direction of the output shaft of the first motor 41, one end of the rotating rod 42 is fixedly connected to the output shaft of the first motor 41, and the other end of the rotating rod 42 is hinged to one end of the connecting rod 43; the connecting rods 43 are distributed perpendicular to the linkage shaft 44, and the other ends of the connecting rods 43 are hinged with the linkage shaft 44; one or more guide rails 12 are arranged on the base 10, a sliding block 13 matched with the guide rails 12 is arranged at the bottom of the moving table 20, and the first motor 41 is controlled by the control panel 11. Therefore, the first motor 41 and the rotating rod 42 are matched to form an eccentric wheel device for providing power, and the power drives the linkage shaft 44 to move through the connecting rod 43; because the linkage shaft 44 is connected with the moving platform 20 as a whole, and the moving platform 20 is guided and limited by the guide rail 12 and the sliding block 13, the movement of the first motor 41 can drive the moving platform 20 to do linear reciprocating movement along the direction of the guide rail 12, and the control panel 11 can adjust and control the linear reciprocating movement speed of the moving platform 20 by controlling the rotating speed of the first motor 41.
As a specific embodiment of the micro-vibration culture apparatus provided in this embodiment, referring to fig. 4 and 5, the second driving mechanism 50 includes a second motor 51, a driving gear 52 and one or more driven gears 53; the second motor 51 is fixedly arranged in the motion table 20; the driving gear 52 is sleeved on an output shaft of the second motor 51, the driven gears 53 are rotatably connected with the motion table 20, the driving gear 52 is in meshing transmission connection with each driven gear 53, the driving gear 52 is in direct meshing transmission with each driven gear 53, or can be in meshing transmission connection with the driven gears 53 after power transmission of one or more transmission gears 54; the top of each driven gear 53 is coaxially and fixedly provided with a rotating disc 30; the second motor 51 is controlled by the control panel 11. Therefore, the driving gear 52 is driven to rotate by the rotation of the second motor 51; the driving gear 52 is engaged with one or more driven gears 53 to drive the plurality of rotating discs 30 to rotate in a circular manner, and the control panel 11 can control the rotating speed of the second motor 51 to adjust and control the speed and frequency of the rotating discs 30 to rotate in a uniform circular manner.
As a specific embodiment of the micro-vibration culture apparatus provided in this embodiment, referring to fig. 4 and 5, the motion stage 20 includes a frame body and a housing 22 covering the outside of the frame body, wherein the frame body includes a bottom plate 211, a top plate 212, and a plurality of fixing shafts 213 installed between the bottom plate 211 and the top plate 212; the second motor 51 is fixedly arranged at the bottom of the top plate 212, an output shaft of the second motor 51 penetrates through the top plate 212 to be connected with the driving gear 52, and the driven gears 53 are rotatably connected to the top plate 212 through a rotating shaft 55; the driving gear 52 and the driven gear 53 are both enclosed in the housing 22, and the rotating disk 30 is exposed from the housing 22. As a further preferred scheme, a frame structure composed of the bottom plate 211, the top plate 212 and the fixing shaft 213 may provide a space for assembling the second motor 51; the top plate 212 may facilitate mounting of the driving gear 52 and the driven gear 53 above the top plate 212; the base plate 211 may facilitate mounting the slider 13 in cooperation with the rail 12 on the base; the frame body is encapsulated and coated by the shell 22, so that the internal structure can be protected, and the appearance attractiveness can be improved; thereby realizing the optimization of the structure and ensuring the working stability of the motion table 20.
Further, as a specific embodiment of the micro-vibration culture apparatus provided in this embodiment, referring to fig. 4 and 5, four driven gears 53 are provided, and the four driven gears 53 are uniformly distributed around the driving gear 52.
Further, as a specific embodiment of the micro-vibration culture apparatus provided in this embodiment, referring to fig. 4 and 5, one or more transmission gears 54 are further disposed between the driving gear 52 and the driven gear 53, and the driving gear 52 is in meshing transmission connection with the driven gear 53 through the transmission gear 54.
As a specific embodiment of the micro-vibration culture apparatus provided in this embodiment, referring to fig. 6, a containing groove 31 for placing a culture dish is opened at the center of the top surface of the rotating disk 30, and two notches 32 are symmetrically distributed around the containing groove 31. The accommodating groove 31 can facilitate the placing of the culture dish, and ensures that the culture dish cannot fall off from the rotating disc 30 in the micro-vibration process, preferably, the depth of the accommodating groove 31 is consistent with the height of the culture dish, so that the stability of the culture dish in the accommodating groove 31 can be improved; meanwhile, the notch 32 is configured to facilitate manual placement of the culture dish into the receiving slot 31 or removal of the culture dish from the receiving slot 31.
Further, as a specific embodiment of the micro-vibration culture apparatus provided in this embodiment, referring to fig. 7, the bottom of the base 10 is provided with a soft rubber pad 14. By utilizing the flexibility of the soft rubber pad 14, especially by adopting the soft rubber pad 14 with certain adsorption capacity, the whole device can be ensured to have a stable motion environment in the process of micro-vibration motion.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (8)
1. A micro-vibration culture device for embryos is characterized in that: the device comprises a base, a moving platform connected to the base in a sliding manner and a rotating disk connected to the moving platform in a rotating manner; a first driving mechanism for driving the moving table to do linear reciprocating motion on the base is arranged in the base; a second driving mechanism for driving the rotating disc to rotate on the moving table is arranged in the moving table; the rotating disc is used for placing a culture dish; the base is further provided with a control panel, and the first driving mechanism and the second driving mechanism are controlled by the control panel.
2. The apparatus for microvibrating culturing of embryos of claim 1, wherein: the first driving mechanism comprises a first motor, a rotating rod, a connecting rod and a linkage shaft; the first motor is fixedly arranged in the base; the linkage shaft is fixedly arranged at the bottom of the motion platform and is distributed in parallel relative to the output shaft of the first motor; the output shaft of the first motor is in transmission connection with the linkage shaft sequentially through a rotary rod and a connecting rod, the rotary rod is distributed along the radial direction of the output shaft of the first motor, one end of the rotary rod is fixedly connected to the output shaft of the first motor, and the other end of the rotary rod is hinged to one end of the connecting rod; the connecting rods are distributed perpendicular to the linkage shaft, and the other ends of the connecting rods are hinged with the linkage shaft;
the base is provided with one or more guide rails, the bottom of the moving platform is provided with a sliding block matched with the guide rails, and the first motor is controlled by the control panel.
3. The apparatus for microvibrating culturing of embryos of claim 1, wherein: the second driving mechanism comprises a second motor, a driving gear and one or more driven gears; the second motor is fixedly arranged in the motion table; the driving gear is sleeved on an output shaft of the second motor, the driven gears are rotatably connected with the motion platform, and the driving gear is in meshing transmission connection with each driven gear; and a rotating disk is coaxially and fixedly arranged at the top of each driven gear, and the second motor is controlled by a control panel.
4. A device for microvibrating culture of embryos according to claim 3, wherein: the motion table comprises a frame body and a shell body coated on the outer side of the frame body, wherein the frame body comprises a bottom plate, a top plate and a plurality of fixing shafts arranged between the bottom plate and the top plate; the second motor is fixedly arranged at the bottom of the top plate, an output shaft of the second motor penetrates through the top plate to be connected with the driving gear, and the driven gears are rotatably connected to the top plate through a rotating shaft; the driving gear and the driven gear are both packaged in the shell, and the rotating disc is exposed out of the shell and distributed.
5. A device for microvibrating culture of embryos according to claim 3, wherein: the driven gears are four and are uniformly distributed around the driving gear.
6. A device for microvibrating culture of embryos according to claim 3, wherein: one or more transmission gears are further arranged between the driving gear and the driven gear, and the driving gear is in meshed transmission connection with the driven gear through the transmission gears.
7. The apparatus for microvibrating culturing of embryos of claim 1, wherein: the top surface center of rotary disk is seted up and is used for placing the storage tank of culture dish, the week side of storage tank is provided with two symmetric distribution's breach portion.
8. The apparatus for microvibrating culturing of embryos of claim 1, wherein: the bottom of the base is provided with a soft rubber cushion.
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| CN201911060778.0A CN110684660A (en) | 2019-11-01 | 2019-11-01 | Micro-vibration culture device for embryos |
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| CN201911060778.0A CN110684660A (en) | 2019-11-01 | 2019-11-01 | Micro-vibration culture device for embryos |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113862149A (en) * | 2021-09-14 | 2021-12-31 | 北京理工大学 | Shimmy integral type cell mechanics loading device |
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