CN114480124B - Multi-dimensional oscillation device for hematopoietic stem cells - Google Patents
Multi-dimensional oscillation device for hematopoietic stem cells Download PDFInfo
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- CN114480124B CN114480124B CN202210092252.6A CN202210092252A CN114480124B CN 114480124 B CN114480124 B CN 114480124B CN 202210092252 A CN202210092252 A CN 202210092252A CN 114480124 B CN114480124 B CN 114480124B
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- sliding block
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- 210000003958 hematopoietic stem cell Anatomy 0.000 title claims abstract description 28
- 230000010355 oscillation Effects 0.000 title abstract description 16
- 230000005540 biological transmission Effects 0.000 claims abstract description 20
- 238000004113 cell culture Methods 0.000 claims abstract description 13
- 238000005192 partition Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 230000009514 concussion Effects 0.000 claims description 9
- 230000002457 bidirectional effect Effects 0.000 claims description 2
- 230000007306 turnover Effects 0.000 abstract description 5
- 210000004027 cell Anatomy 0.000 description 11
- 238000010586 diagram Methods 0.000 description 9
- 238000002156 mixing Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000010370 cell cloning Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003805 vibration mixing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention discloses a multi-dimensional oscillation device for hematopoietic stem cells, which comprises a support column, a box body and a turnover cover, wherein the box body is divided into a culture bin in the middle and transmission bins on two sides through a partition plate, and the device comprises: the middle parts of the top ends of the left baffle plate and the right baffle plate are respectively provided with a chute, and a sliding block is arranged in the chute; the top end of the sliding block is abutted to the top of the sliding groove through an upper vibration spring, the lower end of the sliding block is abutted to the bottom of the sliding groove through a lower vibration spring, and a rotating shaft is rotatably arranged in an inner hole of the sliding block; an eccentric wheel movably fixed on the box body through an eccentric shaft is arranged in the transmission bin, and the edge of the eccentric wheel is connected with a rotating shaft through a swinging rod in hinged connection; the eccentric shaft outside the eccentric wheel is connected with a driving motor arranged at the bottom of the box body in a transmission way. The invention utilizes the sliding block to realize the up-and-down vibration of the test tube bearing plate, utilizes the rotating shaft to realize the left-and-right swinging vibration of the test tube bearing plate along with the eccentric wheel, and realizes the multidimensional vibration of the cell culture tube on the test tube bearing plate; and structural design is simple, novel, labour saving and time saving has improved work efficiency.
Description
Technical Field
The invention relates to the field of cell culture equipment, in particular to a multi-dimensional oscillating device for hematopoietic stem cells.
Background
Cells are the fundamental structural and functional units of organisms, and it is known that all organisms except viruses are composed of cells, but that the vital activity of viruses must also be represented in cells, a biological cell being one of the cells. Cell culture refers to a method of simulating in vitro an in vivo environment (sterile, suitable temperature, ph, certain nutritional conditions, etc.) to survive, grow, reproduce and maintain major structures and functions. Cell culture is also known as cell cloning technology, a formal biological term being cell culture technology.
When culturing biological cells, the biological cells need to be uniformly spread in a culture flask or a culture plate, and thus a mixing treatment using a mixing device is required. However, a plurality of culture tubes can be placed to current vibration mixing device, at cell culture in-process, need artifical manual rocking culture tube, and the mixing effect is not good, can't be effectual with the even shop of cell in the blake bottle, great reduction the work efficiency of device, not reach people's expectation in mind, reduced people's work efficiency, brought inconvenience for people's work.
Disclosure of Invention
The technical problems to be solved by the invention are as follows: aiming at the problems in the prior art, the multi-dimensional oscillation device for the hematopoietic stem cells is provided with high oscillation efficiency and simple and convenient operation.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the invention provides a multi-dimensional oscillation device for hematopoietic stem cells, which comprises a support column 1, a box body 2 and a turnover cover 3, wherein the box body 2 is divided into a culture bin 9 at the middle part and a transmission bin 10 at the left side and the right side through a partition plate 8, wherein:
the middle parts of the top ends of the left baffle plate 8 and the right baffle plate 8 are respectively provided with a chute 11 which is communicated with the culture bin 9 and the transmission bin 10, and a sliding block 12 is arranged in the chute 11; the top end of the sliding block 12 is abutted against the top of the sliding groove 11 through an upper vibration spring 13, the bottom end of the sliding block is abutted against the bottom of the sliding groove 11 through a lower vibration spring 14, and a rotating shaft 15 is rotatably arranged in an inner hole of the sliding block 12;
an eccentric wheel 18 movably fixed on the side wall of the box body 2 through an eccentric shaft 7 is arranged in the transmission bin 10, and the inner side edge of the eccentric wheel 18 is fixedly connected with the rotating shaft 15 through a swing rod 16 which is hinged; the eccentric shaft 7 outside the eccentric wheel 18 is in transmission connection with a driving motor 22 arranged at the bottom of the box body.
Further, on the multi-dimensional oscillation device for hematopoietic stem cells, one end of the rotating shaft 15 is fixedly connected with the test tube bearing plate 4 in the culture bin 9, and a plurality of fixing holes 6 for placing the cell culture tubes 5 are formed in the test tube bearing plate 4.
Further, on the multi-dimensional oscillation device for hematopoietic stem cells, the top end of the oscillating rod 16 is fixedly connected with the rotating shaft 15, and the lower end is provided with a limit groove 17 along the length direction; and a limit column 19 fixed on the edge of the eccentric wheel 18 is embedded in the limit groove 17 in a sliding manner.
Further, on the multi-dimensional oscillation device for hematopoietic stem cells, the cross section of the sliding block 12 is H-shaped, and the sliding block is movably fastened on the partition plates 8 at two sides of the sliding groove 11 and can slide up and down along the sliding groove 11.
Further, on the multi-dimensional oscillation device for hematopoietic stem cells, the eccentric wheel 18 and the eccentric shaft 7 are coaxially arranged, or the eccentric wheel 18 and the eccentric shaft 7 are eccentrically arranged.
Further, in the multi-dimensional oscillation device for hematopoietic stem cells, the driving motor 22 is a bi-directional motor, and driving shafts 23 at two ends of the bi-directional motor are respectively connected with the eccentric shaft 7 through driving belts 20.
Further preferably, on the multi-dimensional oscillating device for hematopoietic stem cells, bottom holes 21 are respectively formed at the bottoms of the two driving bins 10, and the driving belt 20 is arranged through the bottom holes 21.
Further, on the multi-dimensional oscillation device for hematopoietic stem cells, a water inlet pipe 24 and a water outlet pipe 25 are arranged at the back of the box body 2, and the water inlet pipe 24 and the water outlet pipe 25 are both communicated with the culture bin 9.
Further, on the multi-dimensional oscillating device for hematopoietic stem cells, a switch key 27 and a control panel 28 are disposed on the front side of the case 2, and the control panel 28 is electrically connected to the driving motor 22.
Further, on the multi-dimensional oscillating device for hematopoietic stem cells, the rear end edge of the flip cover 3 is hinged to the case 2 by a hinge, and a handle 26 is provided on the top of the front side thereof.
Compared with the prior art, the invention has the following technical effects:
(1) The cell and the cell culture solution are filled in the culture tube when the cell culture device is used, the culture tube is placed in the fixed hole of the test tube rack to be fixed, the servo motor is started through the control panel after the box door is closed, the turntable is driven to rotate in the sliding groove, the transmission rod is driven to move in the movable groove, the connecting rod is driven to swing relative to the test tube rack, and the internal cell culture solution is vibrated and uniformly mixed, so that the aim of mixing is fulfilled, and pollution to internal cells can be avoided;
(2) When the cell is cultured, the constant temperature device can be regulated according to the cell growth requirement, the temperature of the culture cavity is raised and kept at the same time, the temperature in the culture cavity is detected and fed back by arranging the temperature sensor on one side of the culture cavity, and the temperature in the culture cavity is ensured to be constant.
Drawings
FIG. 1 is a schematic diagram showing a three-dimensional structure of a multi-dimensional concussion device for hematopoietic stem cells according to the present invention;
FIG. 2 is a schematic diagram showing the structure of a multi-dimensional concussion device for hematopoietic stem cells according to the present invention;
FIG. 3 is a schematic diagram showing the structure of a multi-dimensional concussion device for hematopoietic stem cells according to the present invention;
FIG. 4 is a schematic diagram showing a rear view of a multi-dimensional concussion device for hematopoietic stem cells according to the present invention;
FIG. 5 is a schematic diagram showing the structure of a transverse cross section of a housing of a multi-dimensional oscillation device for hematopoietic stem cells according to the present invention;
FIG. 6 is a schematic diagram showing a longitudinal cross-sectional structure of a transmission chamber of a multi-dimensional oscillation device for hematopoietic stem cells according to the present invention;
FIG. 7 is a schematic diagram showing a longitudinal cross-sectional structure of the position of a driving chamber in a multi-dimensional oscillation device for hematopoietic stem cells according to the present invention;
FIG. 8 is a schematic diagram of a longitudinal cross-sectional structure of the position of a driving chamber in a multi-dimensional concussion device for hematopoietic stem cells according to the present invention;
FIG. 9 is a schematic diagram showing a longitudinal cross-sectional structure of a driving chamber of a multi-dimensional concussion device for hematopoietic stem cells according to the present invention;
FIG. 10 is a schematic view of a longitudinal cross-sectional structure of a culture chamber of a multi-dimensional concussion device for hematopoietic stem cells according to the present invention;
wherein, each reference sign is:
the device comprises a 1-supporting column, a 2-box body, a 3-turnover cover, a 4-test tube bearing plate, a 5-cell culture tube, a 6-fixing hole, a 7-eccentric shaft, an 8-partition plate, a 9-culture bin, a 10-transmission bin, an 11-sliding chute, a 12-sliding block, a 13-upper vibration spring, a 14-lower vibration spring, a 15-rotating shaft, a 16-swinging rod, a 17-limiting groove, an 18-eccentric wheel, a 19-limiting column, a 20-transmission belt, a 21-bottom hole, a 22-driving motor, a 23-driving shaft, a 24-water inlet pipe, a 25-water outlet pipe, a 26-handle, a 27-on-off key and a 28-control panel.
Detailed Description
The present invention will be described in detail and in detail by way of the following examples, which are not intended to limit the scope of the invention, for better understanding of the invention.
In some embodiments, the invention provides a multi-dimensional oscillation device for hematopoietic stem cells, which comprises a support column 1, a box body 2 and a turnover cover 3, wherein the support column 1 is four corners respectively arranged at the bottom of the box body 2 and is used for supporting the box body 2, a movable wheel can be arranged at the bottom of the support column 1 according to the requirement, and the movable wheel adopts a brake pulley, so that the movement is convenient, and the use flexibility is improved.
The cavity of the box body 2 is divided into a culture bin 9 in the middle and a transmission bin 10 on the left side and the right side through a left partition plate 8 and a right partition plate 8. The middle parts of the top ends of the left baffle plate 8 and the right baffle plate 8 are respectively provided with a chute 11 which is communicated with the culture bin 9 and the transmission bin 10, and a sliding block 12 is arranged in the chute 11; the top of the sliding block 12 is abutted to the top of the sliding groove 11 through an upper vibration spring 13, the bottom of the sliding block 12 is abutted to the bottom of the sliding groove 11 through a lower vibration spring 14, and a rotating shaft 15 is rotatably arranged in an inner hole of the sliding block 12.
An eccentric wheel 18 movably fixed on the side wall of the box body 2 through an eccentric shaft 7 is arranged in the transmission bin 10, and the inner side edge of the eccentric wheel 18 is fixedly connected with the rotating shaft 15 through a swing rod 16 which is hinged; the eccentric shaft 7 outside the eccentric wheel 18 is in transmission connection with a driving motor 22 arranged at the bottom of the box body.
One end of the rotating shaft 15 is fixedly connected with the test tube bearing plate 4 in the culture bin 9, and a plurality of fixing holes 6 for placing the cell culture tubes 5 are formed in the test tube bearing plate 4.
The top end of the swinging rod 16 is fixedly connected with the rotating shaft 15, and the lower end of the swinging rod is provided with a limit groove 17 along the length direction; and a limit column 19 fixed on the edge of the eccentric wheel 18 is embedded in the limit groove 17 in a sliding manner.
The cross section of the sliding block 12 is H-shaped, and the sliding block is movably buckled on the partition plates 8 at two sides of the sliding groove 11 and can slide up and down along the sliding groove 11.
The eccentric wheel 18 is arranged coaxially with the eccentric shaft 7, or the eccentric wheel 18 is arranged eccentrically with the eccentric shaft 7.
The driving motor 22 is a bidirectional motor, and driving shafts 23 at two ends of the driving motor are respectively connected with the eccentric shafts 7 through driving belts 20. And bottom holes 21 are respectively formed in the bottoms of the two transmission bins 10, and the transmission belts 20 are arranged through the bottom holes 21.
A water inlet pipe 24 and a water outlet pipe 25 are arranged on the back of the box body 2, and the water inlet pipe 24 and the water outlet pipe 25 are communicated with the culture bin 9.
The front side of the case 2 is provided with an on/off key 27 and a control panel 28, and the control panel 28 is electrically connected to the driving motor 22. And the rear end edge of the turnover cover 3 is hinged with the box body 2 through a hinge, and the top of the front side of the turnover cover is provided with a handle 26.
The above description of the specific embodiments of the present invention has been given by way of example only, and the present invention is not limited to the above described specific embodiments. Any equivalent modifications and substitutions for the present invention will occur to those skilled in the art, and are also within the scope of the present invention. Accordingly, equivalent changes and modifications are intended to be included within the scope of the present invention without departing from the spirit and scope thereof.
Claims (2)
1. The utility model provides a hematopoietic stem cell multidimension degree concussion device, includes support column (1), box (2) and upset lid (3), its characterized in that, culture bin (9) and drive bin (10) of left and right sides in middle part are cut apart into through baffle (8) to box (2), wherein:
the middle parts of the top ends of the left baffle plate and the right baffle plate (8) are respectively provided with a chute (11) which is communicated with the culture bin (9) and the transmission bin (10), and a sliding block (12) is arranged in the chute (11); the top end of the sliding block (12) is abutted to the top of the sliding groove (11) through an upper vibration spring (13), the bottom end of the sliding block is abutted to the bottom of the sliding groove (11) through a lower vibration spring (14), and a rotating shaft (15) is rotatably arranged in an inner hole of the sliding block (12);
an eccentric wheel (18) movably fixed on the side wall of the box body (2) through an eccentric shaft (7) is arranged in the transmission bin (10), and the inner side edge of the eccentric wheel (18) is fixedly connected with the rotating shaft (15) through a swing rod (16) which is hinged; the eccentric shaft (7) at the outer side of the eccentric wheel (18) is in transmission connection with a driving motor (22) arranged at the bottom of the box body;
one end of the rotating shaft (15) is fixedly connected with a test tube bearing plate (4) in the culture bin (9), and a plurality of fixing holes (6) for placing the cell culture tubes (5) are formed in the test tube bearing plate (4);
the top end of the swinging rod (16) is fixedly connected with the rotating shaft (15), and the lower end of the swinging rod is provided with a limit groove (17) along the length direction; and a limit column (19) fixed on the edge of the eccentric wheel (18) is embedded in the limit groove (17) in a sliding way;
the cross section of the sliding block (12) is H-shaped, and the sliding block is movably buckled on the partition plates (8) at two sides of the sliding groove (11) and can slide up and down along the sliding groove (11);
the eccentric wheel (18) and the eccentric shaft (7) are coaxially arranged, or the eccentric wheel (18) and the eccentric shaft (7) are eccentrically arranged;
the driving motor (22) is a bidirectional motor, and driving shafts (23) at two ends of the driving motor are respectively connected with the eccentric shaft (7) through driving belts (20);
bottom holes (21) are respectively formed in the bottoms of the two transmission bins (10), and the transmission belts (20) penetrate through the bottom holes (21);
a water inlet pipe (24) and a water outlet pipe (25) are arranged at the back of the box body (2), and the water inlet pipe (24) and the water outlet pipe (25) are communicated with the culture bin (9);
the front side of the box body (2) is provided with an on-off key (27) and a control panel (28), and the control panel (28) is electrically connected with the driving motor (22).
2. The multi-dimensional concussion device for hematopoietic stem cells according to claim 1, wherein the rear edge of the flip cover (3) is hinged to the case (2) by a hinge, and a handle (26) is provided on top of the front side thereof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210092252.6A CN114480124B (en) | 2022-01-26 | 2022-01-26 | Multi-dimensional oscillation device for hematopoietic stem cells |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210092252.6A CN114480124B (en) | 2022-01-26 | 2022-01-26 | Multi-dimensional oscillation device for hematopoietic stem cells |
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| Publication Number | Publication Date |
|---|---|
| CN114480124A CN114480124A (en) | 2022-05-13 |
| CN114480124B true CN114480124B (en) | 2024-04-16 |
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| CN202210092252.6A Active CN114480124B (en) | 2022-01-26 | 2022-01-26 | Multi-dimensional oscillation device for hematopoietic stem cells |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210367726U (en) * | 2019-07-15 | 2020-04-21 | 瑞太干细胞中心(沈阳)有限公司 | Multi-dimensional oscillating device for hematopoietic stem cells |
| CN215560380U (en) * | 2021-07-15 | 2022-01-18 | 吉林省银丰生物工程技术有限公司 | Multi-dimensional oscillating device for hematopoietic stem cells |
| CN216864199U (en) * | 2022-01-26 | 2022-07-01 | 上海睿健生物科技有限公司 | Cell culture vibrates mechanism with multidimension degree |
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- 2022-01-26 CN CN202210092252.6A patent/CN114480124B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210367726U (en) * | 2019-07-15 | 2020-04-21 | 瑞太干细胞中心(沈阳)有限公司 | Multi-dimensional oscillating device for hematopoietic stem cells |
| CN215560380U (en) * | 2021-07-15 | 2022-01-18 | 吉林省银丰生物工程技术有限公司 | Multi-dimensional oscillating device for hematopoietic stem cells |
| CN216864199U (en) * | 2022-01-26 | 2022-07-01 | 上海睿健生物科技有限公司 | Cell culture vibrates mechanism with multidimension degree |
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| CN114480124A (en) | 2022-05-13 |
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