CN202201907U - Cell stress culture apparatus - Google Patents
Cell stress culture apparatus Download PDFInfo
- Publication number
- CN202201907U CN202201907U CN 201120319086 CN201120319086U CN202201907U CN 202201907 U CN202201907 U CN 202201907U CN 201120319086 CN201120319086 CN 201120319086 CN 201120319086 U CN201120319086 U CN 201120319086U CN 202201907 U CN202201907 U CN 202201907U
- Authority
- CN
- China
- Prior art keywords
- cell
- spherical crown
- sliding surface
- culturing room
- surface bearing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a cell stress culture apparatus which consists of a cell culture room, a transmission mechanism and a control circuit; a cell is inoculated on a cell culture dish with a silicon membrane at the bottom, and then the cell culture dish is placed in the cell culture room, and a plurality of cell dishes can be placed simultaneously. The rotation of an electromotor in the transmission mechanism drives a mechanical drive structure to ensure that a spherical crown below the cell culture room moves up and down and is in contact with the bottom of the cell culture dish, so as to ensure that the surfaces of the cells cultured in the culture room generates strain. A adjustable potentiometer is rotated in the control circuit to adjust the analog quantity input of a frequency converter, so as to control the frequency of the alternating current output by the frequency converter, thereby changing the rotating speed of the electromotor, driving the drive structure to change the up-and-down movement frequency of the spherical crown, and finally generating the periodic strain with adjustable stress loading frequency in the cell culture room, therefore, the stress to the cells of a human body can be simulated.
Description
Technical field
The utility model relates to a kind of cell to cultivation and carries out stress loaded cells stress culture apparatus, but the suffered stress of anthropomorphic dummy's cells in vivo.
Background technology
At present, cultured cell in vitro has become the technology of a comparative maturity, and under the effect of stress, cell can produce corresponding tractive distortion, and how better analogue body interior mechanics environment is present urgent problem.Mostly domestic traditional cell stress deceleration loading device is four-point bending at present; But when using this mode to carry out the stress loading; Can only carry out stress to the individual cells petridish loads; And nutrient solution can flow when beam deformed back and forth in the petridish, and this fluid force pair cell influence is very big, thereby has limited the accuracy of testing and influenced experimental result.Therefore, hope can have a kind ofly can cultivate many group cells simultaneously, uses special-purpose petridish simultaneously, can let in the cell loading process more evenly, can also realize the static pressurization and the dynamic cell stress culture apparatus of pressurization.
Summary of the invention
Load the shortcoming that number is few, nutrient solution flows the pair cell influence in order to overcome existing cell stress deceleration loading device Tissue Culture Dish; The utility model provides a kind of cell stress culture apparatus; This culture systems can be carried out stress to a plurality of Tissue Culture Dishs simultaneously and loaded, and reduces the influence of the mobile pair cell generation of nutrient solution as far as possible.
The utility model solves the technical scheme that its technical problem adopted:
A kind of cell stress culture apparatus is made up of cell culture chamber, transmission rig and pilot circuit, it is characterized in that:
Cell culture chamber is by cover plate; Cell cultures cell, spherical crown, spherical crown seat, culturing room, bolt, sliding surface bearing A form; Cover plate is fixed by bolts to the inner chamber sealing of culturing room upper end with culturing room; Cell cultures cell, spherical crown, spherical crown seat all are arranged in the inner chamber of culturing room; It is fixing on the cover board that the cultivation cell passes cover plate, and spherical crown is placed in the upper end of spherical crown seat through shrink-fit and is positioned at and cultivates below the cell, and the lower end of spherical crown seat is passed the sliding surface bearing A that is placed in the culturing room bottom and passed culturing room.There are many group spherical crowns available.Different spherical crowns has different spherical crown radiuses and height, shows that Cytology Lab is to bear different strains.
Transmission rig is connected by phonomoter, key, rocking bar, connecting rod, axle B, sliding surface bearing C, axle A, sliding surface bearing B to be realized relatively rotating; It is to form through sliding surface bearing A that the translational motion of forming between spherical crown seat and the culturing room attaches; The axle of phonomoter is connected with an end of rocking bar through key; The other end of rocking bar is connected with an end of connecting rod through axle B and sliding surface bearing C, and the other end of connecting rod is connected with spherical crown seat lower end through axle A and the sliding surface bearing B that is placed in spherical crown seat lower end;
Pilot circuit is made up of power supply, frequency transformer, digital wash, frequency transformer analog quantity output terminal, adjustable potential meter; Regulate the analog quantity input of frequency transformer through turn adjustable potential meter; The frequency of control of conversion device output AC electricity; Change the velocity of rotation of phonomoter with this, and finally regulate the cell stress loading frequency.
With cell inoculation is on the Tissue Culture Dish of pellosil in the bottom, and it is indoor to be placed on cell cultures, can place a plurality of Tissue Culture Dishs simultaneously.Through the rotating band motivation tool drive mechanism of phonomoter, the spherical crown of cell culture chamber below is moved up and down and contact, thereby make cultured cells surface generation strain culturing room in the Tissue Culture Dish bottom.Regulate the analog quantity input of frequency transformer through turn adjustable potential meter; The frequency of control of conversion device output AC electricity; Change the velocity of rotation of phonomoter with this; And drive the frequency that drive mechanism change spherical crown moves up and down, and finally produce the adjustable periodicity strain of culturing cell stress loading frequency in the culturing room.
This cell stress culture apparatus can carry out stress to a plurality of Tissue Culture Dishs simultaneously and load, and has reduced repetitive operation.Reduce the influence of the mobile pair cell of nutrient solution simultaneously, improved the accuracy of experimental result.Can adopt different frequencies to carry out stress to different cells loads, has increased the specific aim of experiment.
Description of drawings
Fig. 1 is cell cultures cell and a spherical crown synoptic diagram of placing Tissue Culture Dish in the utility model;
Fig. 2 is a cell culture chamber synoptic diagram of placing Tissue Culture Dish in the utility model;
Fig. 3 is the mechanical transmission mechanism synoptic diagram that spherical crown moves up and down in the utility model;
Fig. 4 is the pilot circuit synoptic diagram that stress loads in the utility model;
Fig. 5 is the utility model cell stress culture apparatus synoptic diagram;
Wherein 1. cover plates, 2. cell cultures cell, 3. spherical crown, 4. spherical crown seat, 5. culturing room; 6. bolt, 7. sliding surface bearing, 8. an A, 9. sliding surface bearing, 10. connecting rod; 11. sliding surface bearing, 12. B, 13. rocking bars, 14. keys, 15. phonomoter; 16. the motor power input terminal, 17. power supplys, 18. frequency transformers, 19. indicator gauges, 20. analog quantity output terminals, 21. adjustable potential meters.
Embodiment
Further specify below in conjunction with accompanying drawing with to the utility model.
A kind of cell cultures culture apparatus (as shown in Figure 5), form by cell culture chamber, transmission rig and pilot circuit:
Cell culture chamber (as shown in Figure 2) is by cover plate 1; Cell cultures cell 2 is formed with spherical crown 3 (as shown in Figure 1), spherical crown seat 4, culturing room 5, bolt 6, sliding surface bearing A 7; Cover plate is fixed by bolts to the inner chamber sealing of culturing room upper end with culturing room; Cell cultures cell, spherical crown, spherical crown seat all are arranged in the inner chamber of culturing room; It is fixing on the cover board that the cultivation cell passes cover plate, and spherical crown is placed in the upper end of spherical crown seat through shrink-fit and is positioned at and cultivates below the cell, and the lower end of spherical crown seat is passed the sliding surface bearing A that is placed in the culturing room bottom and passed culturing room.
Cell culture chamber is made up of a plurality of cell cultures cells 2, and cultivate cell and be fixed on the cover plate 1, be a spherical crown 3 below the cell cultures cell, through the periodically collision of the regular pair cell cell that moves up and down of spherical crown, make cell bear periodically strain.
There are many group spherical crowns available.Different spherical crowns has different spherical crown radiuses and height, shows that Cytology Lab is to bear different strains.
Spherical crown is to be installed on the spherical crown seat 4 through shrink-fit, and is that the translational motion that slides attaches between spherical crown seat 4 and the culturing room 5.Cover plate is fixed in the culturing room 5 through bolt 6, cultivates the stationkeeping of cell with respect to culturing room like this.
Transmission rig (as shown in Figure 3) is connected by phonomoter 15, key 14, rocking bar 13, connecting rod 10, axle B 12, sliding surface bearing C 11, axle A 8 and sliding surface bearing B 9 to be realized relatively rotating; It is to form through sliding surface bearing A 7 that the translational motion of forming between spherical crown seat and the culturing room attaches; The axle of phonomoter 15 is connected with an end of rocking bar 13 through key 14; The other end of rocking bar 13 is connected with an end of connecting rod 10 through axle B 12 and sliding surface bearing C 11, and the other end of connecting rod 10 is connected with spherical crown seat lower end through axle A 8 and the sliding surface bearing B 9 that is placed in spherical crown seat lower end.
Along with the rotation of phonomoter 15, rotate through key 14 and motor shaft fixed rocking bar 13, rocking bar drivening rod 10 is done swing, moves up and down thereby connecting rod drive spherical crown seat is done.Be to be connected through axle B 12 and sliding surface bearing 11 to realize relatively rotating between rocking bar and the connecting rod; Be to be connected through axle A8 and sliding surface bearing 9 to realize relatively rotating between connecting rod and the spherical crown seat; The translational motion of forming between spherical crown seat and the culturing room attaches through sliding surface bearing 7 to be realized moving up and down.
Pilot circuit (as shown in Figure 4) is made up of power supply 17, frequency transformer 18, digital wash 19, frequency transformer analog quantity output terminal 20, adjustable potential meter 21; Power supply 17 is connected through power cable with frequency transformer 18 each other; Digital wash 19, frequency transformer analog quantity output terminal 20, adjustable potential meter 21 connect through power cable each other; Regulate the analog quantity input of frequency transformer through turn adjustable potential meter; The frequency of control of conversion device output AC electricity changes the velocity of rotation of phonomoter with this, and finally regulates the cell stress loading frequency.
With cell inoculation is on the Tissue Culture Dish of pellosil in the bottom, and it is indoor to be placed on cell cultures, can place a plurality of Tissue Culture Dishs simultaneously.Through the rotating band motivation tool drive mechanism of phonomoter, the spherical crown of cell culture chamber below is moved up and down and contact, thereby make cultured cells surface generation strain culturing room in the Tissue Culture Dish bottom.Regulate the analog quantity input of frequency transformer through turn adjustable potential meter; The frequency of control of conversion device output AC electricity; Change the velocity of rotation of phonomoter with this; And drive the frequency that drive mechanism change spherical crown moves up and down, and finally produce the adjustable periodicity strain of culturing cell stress loading frequency in the culturing room.
The beneficial effect of the utility model is, can carry out stress to a plurality of Tissue Culture Dishs simultaneously and load, and reduced repetitive operation.Reduce the influence of the mobile pair cell of nutrient solution simultaneously, improved the accuracy of experimental result.Can adopt different frequencies to carry out stress to different cells loads, has increased the specific aim of experiment.
Claims (1)
1. a cell stress culture apparatus is made up of cell culture chamber, transmission rig and pilot circuit, it is characterized in that:
Cell culture chamber is by cover plate; Cell cultures cell, spherical crown, spherical crown seat, culturing room, bolt, sliding surface bearing A form; Cover plate is fixed by bolts to the inner chamber sealing of culturing room upper end with culturing room; Cell cultures cell, spherical crown, spherical crown seat all are arranged in the inner chamber of culturing room; It is fixing on the cover board that the cultivation cell passes cover plate, and spherical crown is placed in the upper end of spherical crown seat through shrink-fit and is positioned at and cultivates below the cell, and the lower end of spherical crown seat is passed the sliding surface bearing A that is placed in the culturing room bottom and passed culturing room;
Transmission rig is connected by phonomoter, key, rocking bar, connecting rod, axle B, sliding surface bearing C, axle A, sliding surface bearing B to be realized relatively rotating; It is to form through sliding surface bearing A that the translational motion of forming between spherical crown seat and the culturing room attaches; The axle of phonomoter is connected with an end of rocking bar through key; The other end of rocking bar is connected with an end of connecting rod through axle B and sliding surface bearing C, connecting rod the other end be connected with spherical crown seat lower end through axle A and the sliding surface bearing B that is placed in spherical crown seat lower end;
Pilot circuit is made up of power supply, frequency transformer, digital wash, frequency transformer analog quantity output terminal, adjustable potential meter; Power supply is connected through power cable with frequency transformer each other, and digital wash, frequency transformer analog quantity output terminal, adjustable potential meter connect through power cable each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120319086 CN202201907U (en) | 2011-04-11 | 2011-08-29 | Cell stress culture apparatus |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120103260.3 | 2011-04-11 | ||
| CN201120103260 | 2011-04-11 | ||
| CN 201120319086 CN202201907U (en) | 2011-04-11 | 2011-08-29 | Cell stress culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202201907U true CN202201907U (en) | 2012-04-25 |
Family
ID=45966039
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120319086 Expired - Fee Related CN202201907U (en) | 2011-04-11 | 2011-08-29 | Cell stress culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202201907U (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103992947A (en) * | 2014-05-27 | 2014-08-20 | 中国人民解放军第四军医大学 | Loading device and method for pressure stress of skeletal cells in porous titanium alloy |
| CN109706079A (en) * | 2018-12-11 | 2019-05-03 | 江苏理工学院 | It is a kind of for simulating the flow cavity of flowing environment after bracket |
| CN109777737A (en) * | 2019-02-01 | 2019-05-21 | 广州迈普再生医学科技股份有限公司 | A kind of pulsation incubator and perfused culture system |
| CN110577895A (en) * | 2019-10-14 | 2019-12-17 | 东华大学 | dynamic cell culture method and culture device for simulating in-vivo dynamic environment |
| CN110904090A (en) * | 2019-11-25 | 2020-03-24 | 东华大学 | Dynamic cell culture method and culture device simulating in-vivo force-electricity microenvironment |
| CN111748474A (en) * | 2020-07-14 | 2020-10-09 | 杭州百桥医疗技术有限公司 | Elastic soft bottom equal stress strain cell dynamic culture device |
| CN113604358A (en) * | 2021-07-29 | 2021-11-05 | 中国科学院长春光学精密机械与物理研究所 | Cell loading device capable of replacing plane force and pneumatic control method |
| CN113862149A (en) * | 2021-09-14 | 2021-12-31 | 北京理工大学 | Shimmy integral type cell mechanics loading device |
-
2011
- 2011-08-29 CN CN 201120319086 patent/CN202201907U/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103992947A (en) * | 2014-05-27 | 2014-08-20 | 中国人民解放军第四军医大学 | Loading device and method for pressure stress of skeletal cells in porous titanium alloy |
| CN109706079B (en) * | 2018-12-11 | 2022-03-25 | 江苏理工学院 | Flow cavity for simulating flowing environment behind bracket |
| CN109706079A (en) * | 2018-12-11 | 2019-05-03 | 江苏理工学院 | It is a kind of for simulating the flow cavity of flowing environment after bracket |
| CN109777737A (en) * | 2019-02-01 | 2019-05-21 | 广州迈普再生医学科技股份有限公司 | A kind of pulsation incubator and perfused culture system |
| CN109777737B (en) * | 2019-02-01 | 2023-01-10 | 广州迈普再生医学科技股份有限公司 | Pulsation incubator and perfusion culture system |
| CN110577895A (en) * | 2019-10-14 | 2019-12-17 | 东华大学 | dynamic cell culture method and culture device for simulating in-vivo dynamic environment |
| CN110904090B (en) * | 2019-11-25 | 2021-10-08 | 东华大学 | Dynamic cell culture method and culture device simulating in-vivo force-electricity microenvironment |
| CN110904090A (en) * | 2019-11-25 | 2020-03-24 | 东华大学 | Dynamic cell culture method and culture device simulating in-vivo force-electricity microenvironment |
| CN111748474A (en) * | 2020-07-14 | 2020-10-09 | 杭州百桥医疗技术有限公司 | Elastic soft bottom equal stress strain cell dynamic culture device |
| CN111748474B (en) * | 2020-07-14 | 2023-01-10 | 杭州百桥医疗技术有限公司 | Elastic soft bottom equal stress strain cell dynamic culture device |
| CN113604358A (en) * | 2021-07-29 | 2021-11-05 | 中国科学院长春光学精密机械与物理研究所 | Cell loading device capable of replacing plane force and pneumatic control method |
| CN113862149A (en) * | 2021-09-14 | 2021-12-31 | 北京理工大学 | Shimmy integral type cell mechanics loading device |
| CN113862149B (en) * | 2021-09-14 | 2023-10-27 | 北京理工大学 | Shimmy integrated cell mechanics loading device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN202201907U (en) | Cell stress culture apparatus | |
| CN106245691B (en) | Calcareous sand sound load Multifunctional pile base model test apparatus | |
| CN104565191B (en) | Double-intelligent flexible beam device based on driving and oscillating of planetary reducer | |
| CN201984693U (en) | Wave generation device for sea wave power generation researches | |
| CN103305424B (en) | Variable-amplitude rotary cell culture table | |
| CN101221166B (en) | Cell strain loading device under three-dimensional cultivation condition | |
| CN111117890A (en) | Cell dynamic tension stress culture device | |
| CN105543092A (en) | Dynamic non-contact cell mechanical stimulation loading device | |
| CN201908092U (en) | Cell cyclic compression and tension device | |
| CN110514402A (en) | A kind of experimental provision of wave energy apparatus performance test | |
| CN105331537B (en) | A kind of Mouthsimulator chews the cell culture apparatus of mechanical environment | |
| CN104655417B (en) | Device for testing high and low temperature performance of certain indexing mechanism of space station | |
| CN103555561A (en) | Biomechanical generator | |
| CN205188307U (en) | Mechanical environment's cell culture device is chewed in simulation oral cavity | |
| CN107942726A (en) | Magnetic suspension ball rod system semi-physical simulation experiment platform based on rapid model | |
| CN203534896U (en) | Cam quantitative repeated loading device | |
| CN202246701U (en) | Low-frequency alternating magnetic field assisted fermentation shaking table experimental device | |
| CN203284409U (en) | Miniature device for culturing large flux microorganisms | |
| CN103525701B (en) | Tissue engineering cartilago articularis in-vitro construction method and device | |
| CN201149589Y (en) | Large displacement cell strain loading device under three-dimensional cultivation condition | |
| CN103289898B (en) | Elliptical orbit type cell culture oscillator | |
| CN201149590Y (en) | Large active force cell strain loading device under three-dimensional cultivation condition | |
| CN101092595B (en) | Experimental apparatus for loading cell through digital controlled mechanical strain | |
| CN203746337U (en) | Mechanical drive performance comprehensive test on-line contrast test bench for teaching | |
| CN208494311U (en) | A kind of biology laboratory test tube rocking equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120425 Termination date: 20140829 |
|
| EXPY | Termination of patent right or utility model |