CN201610428U - Cell stretching device with continuously-adjustable strain - Google Patents
Cell stretching device with continuously-adjustable strain Download PDFInfo
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- CN201610428U CN201610428U CN2009202938340U CN200920293834U CN201610428U CN 201610428 U CN201610428 U CN 201610428U CN 2009202938340 U CN2009202938340 U CN 2009202938340U CN 200920293834 U CN200920293834 U CN 200920293834U CN 201610428 U CN201610428 U CN 201610428U
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Abstract
The utility model discloses a cell stretching device with continuously-adjustable strain, which comprises a frame, a motor arranged at one end of the frame, a baffle plate fixedly arranged at the other end of the frame, a guide track arranged longitudinally along the frame, a sliding plate arranged in sliding fit with the guide track, and at least one elastic base arranged between the sliding plate and the baffle plate in a way that one end of the elastic base is connected with the sliding plate and the other end thereof is connected with the baffle plate, wherein a crank mechanism with adjustable eccentric distance is arranged in a fixed fit in the circumferential direction of the rotating shaft of the motor, and the reciprocating power output end of the crank mechanism is in fixed fit with the sliding plate in the longitudinal direction of the frame. The motor drives the elastic base to make stretching motion through the crank mechanism and thus to adjust the position of a sliding arm inside a sliding chute and change the eccentric distance of the crank mechanism, thereby changing the tensile deformation of the elastic base, as a result, the cells attached to the carrier membrane of the elastic base are stretched passively and uniformly. The cell stretching device with continuously-adjustable strain can achieve the static or periodical mechanical load with continuously adjustable stretching time, frequency and strain on different types of cells at the same time.
Description
Technical field
The utility model relates to a kind of cyto-mechanics deceleration loading device, the cell tensile device that particularly a kind of strain continuous is adjustable.
Background technology
Along with the development of biomechanics and organizational project, cyto-mechanics has become the focus of biomedical research.The cultured cell in vitro mechanics loaded can not only get rid of the effect that the cells in vivo Experiments of Machanics are difficult to distinguish mechanics factor and other factors pair cell, and under can also the effect of analogue body internal stress in cell and the three-dimensional rack mechanical response of cell and the influence of Growth and Differentiation function be the important method of cyto-mechanics research.
At present in all multi-methods that the cell in vitro mechanics loads, substrate deformation loading technique is substrate with the elastica, by mechanical stretching or utilize gas, hydraulic coupling to cause elastic substrates deformation, make the passive stretching of the cell that is attached to substrate, meet cell loading characteristic in the interior most cell or tissue engineering racks of body, not having practical cycle limit, is a kind of comparatively ideal experimental facilities.In existing substrate deformation deceleration loading device, as publication number is the CN200999244Y Chinese patent, disclosed a kind of cell based bill kept on file axle stretching device, though can implement moving/static mechanical to the rectangular base film stretches, but the basilar membrane deformation quantity is a fixed, that is to say can not the analogue body inner cell to the response of the big or small stress of difference, and will stick and have the elastic substrates of cell film to be fixed to operational difficulty on the device, easy damaged cells.
And for example the elastica in the substrate deformation deceleration loading device of U.S. Pat 5348879 (A) and Chinese patent CN101092595A disclosure is fixing on every side, and the area fixed machinery top board below elastica moves up and down and changes the elastica tensile deformation.The shortcoming of this mode is the unbalance stress of film, causes the cell unbalance stress, particularly when curved roof does not have complete contact resilient film, has only film middle stressed.
Therefore, need a kind of cyto-mechanics deceleration loading device, both be convenient to cell cultures and loaded, can make things convenient for again and regulate the elastica tensile deformation continuously, make cell stressed in the course of the work evenly, it is reliable to operate steadily, and good reproducibility is easy to maintenance.
The utility model content
In view of this, the utility model provides a kind of strain continuous adjustable cell tensile device, both has been convenient to cell cultures and has loaded, can make things convenient for again and regulate the elastica tensile deformation continuously, make cell stressed in the course of the work evenly, it is reliable to operate steadily, good reproducibility, easy to maintenance.
The cell tensile device that strain continuous of the present utility model is adjustable, comprise frame and the motor that is installed on frame one end, the frame the other end fixedly installs baffle plate, along stands longitudinal guide rail is set, be slidingly matched with guide rail slide plate is set, at least one elastic matrix is set between described slide plate and the baffle plate, and elastic matrix one end is connected with slide plate, and the other end is connected with baffle plate; The rotating shaft of described motor is provided with sliding chute seat along circumferential heavy keying fit, and sliding chute seat is provided with chute, and being slidingly matched with chute is provided with cursor slide, forms the adjustable crank mechanism of eccentricity, and locking latches is set on the cursor slide; The to-and-fro movement clutch end of described crank mechanism and slide plate are along the stands longitudinal heavy keying fit.
Further, described cursor slide is provided with axially extending bore, and circle being provided with internal thread in the through hole, and described locking latches is placed in the swing arm of cursor slide in being, the swing arm outside surface is provided with the screw-internal thread fit of outside screw and through hole, and the swing arm tail end is a boss structure;
Further, return spring longitudinally is set between slide plate and the baffle plate, described clutch end is a push rod, the L shaped structure of the vertical mutually formation in push rod two ends, its horizontal ends and slide plate be heavy keying fit longitudinally, it is vertically held vertical with swing arm and be close to swing arm under the effect of return spring preliminary tension, constitutes the crank post rod mechanism with swing arm;
Further, the contact part of described swing arm and L shaped push rod is provided with bearing along circumferential heavy keying fit;
Further, described clutch end is a connecting rod, and connecting rod one end and swing arm are hinged, and the other end and slide plate are hinged and constitute toggle with swing arm;
Further, described chute upper edge slip direction is provided with scale marks;
Further, described elastic matrix is the elastic silica gel cell culture chamber;
Further, also comprise controller and driving mechanism, controller, driving mechanism and motor are electrically connected successively;
Further, described controller is computer, micro-chip or programmable logic controller;
Further, described motor is a stepper-motor.
The beneficial effects of the utility model: motor can drive elastic matrix by crank mechanism and make stretching motion, regulate the position of cursor slide on chute, can change the crank mechanism eccentricity, and then the tensile deformation amount of change elastic matrix, make the cell that attaches to the elastic matrix counterdie be subjected to uniform passive tractive, can be simultaneously to multiple different cells realize stretching times, frequency, strain continuous is adjustable static or periodically mechanics load.
Description of drawings
Below in conjunction with the drawings and specific embodiments the utility model is further described.
Fig. 1 is the structural representation of the utility model structure;
Fig. 2 is that the A-A of Fig. 1 is to sectional view;
Fig. 3 is that the B-B of Fig. 1 is to sectional view;
Fig. 4 is that the C-C of Fig. 1 is to sectional view;
Fig. 5 is the synoptic diagram of the another kind of structure of the utility model;
Fig. 6 is that the D-D of Fig. 5 is to sectional view.
Embodiment
Fig. 1 is the structural representation of the utility model structure, Fig. 2 be the A-A of Fig. 1 to sectional view, Fig. 3 be the B-B of Fig. 1 to sectional view, Fig. 4 is that the C-C of Fig. 1 is to sectional view; As shown in the figure: the cell tensile device that the strain continuous of present embodiment is adjustable, comprise frame 1 and the motor 2 that is installed on frame 1 one ends, frame 1 the other end fixedly installs baffle plate 3, along stands longitudinal guide rail 4 is set, guide rail 4 is two metallic rod that vertically are provided with, the above-below direction of described vertical finger Fig. 1, be slidingly matched with guide rail 4 slide plate 5 is set, certainly, guide rail can be equal to replacement with chute, can realize the purpose of this utility model equally, and four elastic matrixes are set between described slide plate 5 and the baffle plate 3, can load many groups cell simultaneously, certainly, elastic matrix also can be provided with one or more according to actual needs, and elastic matrix can adopt elastica or other any resilient materials that is suitable for the cell growth, elastic matrix one end and slide plate 5 sockets, the other end and baffle plate 3 sockets; The rotating shaft of described motor 2 is provided with sliding chute seat 7 along circumferential heavy keying fit, sliding chute seat 7 is provided with chute, be slidingly matched with chute cursor slide 8 is set, sliding chute seat rotation under driven by motor, cursor slide forms the adjustable crank mechanism of eccentricity around the revolution of machine shaft axis, and locking latches also is set on the cursor slide 8, cursor slide can be locked, relatively sliding does not take place; The to-and-fro movement clutch end of described crank mechanism and slide plate 5 can drive slide plate along the stands longitudinal to-and-fro movement along frame 1 vertical heavy keying fit.
In the present embodiment, described cursor slide 8 is provided with axially extending bore, circle is provided with internal thread in the through hole, described locking latches is placed in the swing arm 9 of cursor slide 8 in being, swing arm 9 outside surfaces are provided with the screw-internal thread fit of outside screw and through hole, and swing arm 9 tail ends are boss structure, by the rotation swing arm, make boss structure compress the chute bottom, rely on the frictional force between raised head face and the chute bottom to play the effect of locking cursor slide.
In the present embodiment, between slide plate 5 and the baffle plate 3 return spring 10 is set longitudinally, described clutch end is a push rod 11, the L shaped structure of the vertical mutually formation in push rod 11 two ends, its horizontal ends and slide plate 5 be heavy keying fit longitudinally, in the present embodiment with push rod by screw retention on slide plate, vertically end and swing arm 9 are vertical and be close to swing arm 9 under return spring 10 preliminary tension effects for they, constitute the crank post rod mechanism with swing arm 9, as shown in Figure 1, adopt swing arm to be arranged on the mode in push rod the place ahead, return spring need use extension spring so, otherwise, being arranged on the push rod rear as swing arm, return spring need use stage clip.
In the present embodiment, described swing arm 9 is provided with bearing 13 with the contact part of push rod 11 along circumferential heavy keying fit, makes bearing and push rod form rolling pair, reduce collision and friction, improve the stability of device operation, can also rubber be set, further reduce collision at bearing outside.
Certainly, transmission of the present utility model can also realize by another kind of mode, as shown in Figure 5 and Figure 6, described clutch end is a connecting rod 12, connecting rod 12 1 ends and swing arm 9 are hinged, and the other end and slide plate 5 hinged and swing arm 9 formation toggles can be realized the purpose of this utility model equally.
In the present embodiment, described sliding chute seat 7 upper edge slip directions are provided with scale marks, can carry out adjustment directly perceived and quantitative to eccentricity, are convenient to the arrangement and the record of data.
In the present embodiment, described elastic matrix is an elastic silica gel cell culture chamber 14, on four jiaos of the elastic silica gel cell culture chamber through hole is set, can by be provided with on baffle plate and the slide plate hook or pin the elastic silica gel cell culture chamber is hung between baffle plate and the slide plate, this cultivation box can have been bought on the market easily, and telescopicing performance is good.
In the present embodiment, also comprise controller 15 and driving mechanism 16, controller 15, driving mechanism 16 and motor 2 are electrically connected successively, controller and driving mechanism can be fixed with frame, also can be set to the place that other are convenient to operate, controller is used to export control signal, and control signal process driving mechanism is with power magnification, control motor corner, and then control stretching frequency and time.
In the present embodiment, described controller 15 is computer, micro-chip or programmable logic controller.
In the present embodiment, described motor 2 is a stepper-motor, and precision is higher, and controllability is good.
Explanation is at last, above embodiment is only unrestricted in order to the explanation the technical solution of the utility model, although the utility model is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement the technical solution of the utility model, and not breaking away from the aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of the claim scope of the present utility model.
Claims (10)
1. cell tensile device that strain continuous is adjustable, it is characterized in that: comprise frame (1) and be installed on the motor (2) of frame (1) one end, frame (1) the other end fixedly installs baffle plate (3), along stands longitudinal guide rail (4) is set, be slidingly matched with guide rail (4) slide plate (5) is set, between described slide plate (5) and the baffle plate (3) at least one elastic matrix is set, elastic matrix one end is connected with slide plate (5), and the other end is connected with baffle plate (3); The rotating shaft of described motor (2) is provided with sliding chute seat (7) along circumferential heavy keying fit, and sliding chute seat (7) is provided with chute, and being slidingly matched with chute is provided with cursor slide (8), forms the adjustable crank mechanism of eccentricity, and cursor slide also is provided with locking latches on (8); The to-and-fro movement clutch end of described crank mechanism and slide plate (5) are along the vertical heavy keying fit of frame (1).
2. the cell tensile device that strain continuous according to claim 1 is adjustable, it is characterized in that: described cursor slide (8) is provided with axially extending bore, circle is provided with internal thread in the through hole, described locking latches is placed in the swing arm (9) of cursor slide (8) in being, swing arm (9) outside surface is provided with the screw-internal thread fit of outside screw and through hole, and swing arm (9) tail end is a boss structure.
3. the cell tensile device that strain continuous according to claim 2 is adjustable, it is characterized in that: between slide plate (5) and the baffle plate (3) return spring (10) is set longitudinally, described clutch end is push rod (11), the L shaped structure of the vertical mutually formation in push rod (11) two ends, its horizontal ends and slide plate (5) be heavy keying fit longitudinally, it is vertically held vertical with swing arm (9) and be close to swing arm (9) under return spring (10) preliminary tension effect, constitutes the crank post rod mechanism with swing arm (9).
4. the cell tensile device that strain continuous according to claim 3 is adjustable is characterized in that: described swing arm (9) is provided with bearing (13) with the contact part of push rod (11) along circumferential heavy keying fit.
5. the cell tensile device that strain continuous according to claim 2 is adjustable, it is characterized in that: described clutch end is connecting rod (12), connecting rod (12) one ends and swing arm (9) are hinged, and the other end and slide plate (5) are hinged and constitute toggle with swing arm (9).
6. the cell tensile device adjustable according to each described strain continuous of claim 3 to 5 is characterized in that: described sliding chute seat (7) upper edge slip direction is provided with scale marks.
7. the cell tensile device that strain continuous according to claim 6 is adjustable is characterized in that: described elastic matrix is elastic silica gel cell culture chamber (14).
8. the cell tensile device that strain continuous according to claim 7 is adjustable is characterized in that: also comprise controller (15) and driving mechanism (16), controller (15), driving mechanism (16) and motor (2) are electrically connected successively.
9. the cell tensile device that strain continuous according to claim 8 is adjustable is characterized in that: described controller (15) is computer, micro-chip or programmable logic controller.
10. the cell tensile device that strain continuous according to claim 9 is adjustable is characterized in that: described motor (2) is a stepper-motor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202938340U CN201610428U (en) | 2009-12-16 | 2009-12-16 | Cell stretching device with continuously-adjustable strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202938340U CN201610428U (en) | 2009-12-16 | 2009-12-16 | Cell stretching device with continuously-adjustable strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201610428U true CN201610428U (en) | 2010-10-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009202938340U Expired - Fee Related CN201610428U (en) | 2009-12-16 | 2009-12-16 | Cell stretching device with continuously-adjustable strain |
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| Country | Link |
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| CN (1) | CN201610428U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250753A (en) * | 2011-05-25 | 2011-11-23 | 北京生命科学研究所 | Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof |
| CN108732049A (en) * | 2018-03-26 | 2018-11-02 | 东莞市恒宇仪器有限公司 | A kind of tensile fatigue test machine |
| CN109468225A (en) * | 2018-11-12 | 2019-03-15 | 太原理工大学 | A kind of cell mechanical tensioning devices of real time data feedback |
| CN110904090A (en) * | 2019-11-25 | 2020-03-24 | 东华大学 | Dynamic cell culture method and culture device simulating in-vivo force-electricity microenvironment |
| CN115125122A (en) * | 2022-06-06 | 2022-09-30 | 太原理工大学 | Single cell three-dimensional dynamic mechanical stimulation device |
-
2009
- 2009-12-16 CN CN2009202938340U patent/CN201610428U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250753A (en) * | 2011-05-25 | 2011-11-23 | 北京生命科学研究所 | Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof |
| CN102250753B (en) * | 2011-05-25 | 2014-02-05 | 北京生命科学研究所 | Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof |
| CN108732049A (en) * | 2018-03-26 | 2018-11-02 | 东莞市恒宇仪器有限公司 | A kind of tensile fatigue test machine |
| CN109468225A (en) * | 2018-11-12 | 2019-03-15 | 太原理工大学 | A kind of cell mechanical tensioning devices of real time data feedback |
| CN110904090A (en) * | 2019-11-25 | 2020-03-24 | 东华大学 | Dynamic cell culture method and culture device simulating in-vivo force-electricity microenvironment |
| CN115125122A (en) * | 2022-06-06 | 2022-09-30 | 太原理工大学 | Single cell three-dimensional dynamic mechanical stimulation device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101020 Termination date: 20121216 |