CN109666589A - A kind of dynamic cell culture device - Google Patents

Abstract

The present invention relates to experimental instrument fields, disclose a kind of dynamic cell culture device, the dynamic cell culture device includes tissue culture plate and oscillation device, and the tissue culture plate includes supporting plate, cell culture well, first driving device, support base, the first drive shaft, first gear, connecting rod, culture foundation trench, preformed hole and impeller；The oscillation device includes including oscillation device ontology, pallet, the second driving device, the second drive shaft, second gear and lever；Baffle is surrounded by the pallet of the oscillation device, the supporting plate of the tissue culture plate is nested in the cavity that surrounding baffle is encircled on the pallet.It is static culture cell that dynamic cell culture device of the invention, which solves existing most cells culture apparatus, can not the intracorporal dynamic environment of analog machine to cell carry out dynamic cultivation the problem of, have the advantages that it is small in size, easy to use, experimental result accuracy and science can be improved.

Description

A kind of dynamic cell culture device

Technical field

The present invention relates to experimental instrument fields, more particularly to a kind of dynamic cell culture device.

Background technique

Most of common cell culture apparatus in laboratory is static culture device at present, and culture medium is static, cell It is cultivated under static environment, and in animal body, tissue fluid can flow, therefore cell is moved in dynamic environment. Cell state, function for cultivating on quiescence cells culture apparatus etc. are all variant with the lower culture of dynamic, and experimental result may also It can be variant with the intracorporal cell detection results of animal.Therefore, it is necessary in a dynamic environment using dynamic cell culture device Cell is cultivated, cell normal function, differentiation, secretion etc. is maintained, improves the accuracy and science of experimental result.

Also have a kind of instrument on the market, can to cultivate cell under flow regime, however mainly for detection of hydrodynamic shear, It is a kind of fluid shearing Force system, disadvantage is that: 1. is not suitable for various experiments, and use scope is small；2. instrument valence Lattice are expensive, and cell plates are also more expensive, at high cost；3. the auxiliary device for needing to connect when use is more, inconvenient to use；4. body Product is big, and inconvenience is put into cell incubator or super-clean bench and uses.

Since the living environment of cell in animal body is dynamic, and animal body is in most cases in movement shape Therefore state is researched and developed a kind of small in size, easy to use, can simulate the true living environment of cell and the mass motion phase of animal body In conjunction with dynamic environment cultivate cell, to improve experimental result accuracy and scientific dynamic cell culture device actually It is necessary.

Summary of the invention

The present invention in order to overcome at least one of the drawbacks of the prior art described above (deficiency), provide it is a kind of it is small in size, at This dynamic cell culture device low, easy to use.

In order to solve the above technical problems, the technical scheme adopted by the invention is as follows:

A kind of dynamic cell culture device, which is characterized in that the dynamic cell culture device includes tissue culture plate and oscillation Device:

The tissue culture plate includes supporting plate, cell culture well, first driving device, support base, the first drive shaft, the first tooth Wheel, connecting rod, culture foundation trench, preformed hole and impeller, the first driving device are connect with support base, the support base and support Plate connection, first drive shaft connect with first driving device, and the first driving device passes through the first drive shaft drive the One gear and connecting rod rotate, and the connecting rod is through culture medium groove sidewall and connection boom end is provided with impeller, the culture Foundation trench is internally provided with cell culture well and preformed hole；

The oscillation device include including oscillation device ontology, pallet, the second driving device, the second drive shaft, second gear with And lever, the second driving device setting are filled in oscillation device body interior, second drive shaft one end with the second driving Connection is set, the other end of the second drive shaft is connect with second gear center, the tooth top of the second gear and the lever contact, The both ends of the lever are flexibly connected with oscillation device ontology, and the pallet is connected to the middle part of lever by pillar；

Baffle is surrounded by the pallet of the oscillation device, the supporting plate of the tissue culture plate is nested in surrounding on the pallet In the cavity that baffle is encircled into.Clamping unit is provided between the pallet and supporting plate, the clamping unit is gear needle, the gear One end of needle is flexibly connected with baffle, and the other end for keeping off needle can be stirred, and supporting plate can be pressed on pallet, the clamping unit It is settable multiple between supporting plate and pallet.

Dynamic cell culture device volume of the invention is small, at low cost, easy to use, simulates the true living environment of cell Cell is cultivated, to improve experimental result accuracy and science.

Further, the culture foundation trench of the tissue culture plate, which is square, is symmetrically distributed on supporting plate, first driving Device is set to support base top, and first drive shaft one end is connect with first driving device, the other end of the first drive shaft Be flexibly connected with the support rod being arranged on supporting plate, the first drive shaft surface spaced set has an external screw thread, the external screw thread with The first gear engagement being fixedly installed in the middle part of connecting rod, the both ends of the connecting rod extend through corresponding culture medium groove sidewall And the both ends of connecting rod are provided with impeller.Culture foundation trench is square distribution, is adapted to more experimental occasions.Using gear The kind of drive, not only may be implemented the deflecting of driving force, but also have the characteristics that stable transmission, high-efficient, compact-sized.

Further, the quantity of the culture foundation trench of the tissue culture plate experimental can need in even numbers increase or It reduces, two is symmetrical arranged at least on supporting plate, one cell culture well of setting and a preformed hole in the culture foundation trench.Carefully Born of the same parents' culture hole is used to cultivate attached cell, and setting preformed hole both can use its contour structures and form culture medium in culture foundation trench Special flow passage, can also in preformed hole static culture cell, or the controlled observation area as cell culture.

Further, two cell culture wells and a preformed hole also can be set in the culture foundation trench.Setting two The purpose of cell culture well is for cultivating two kinds of cells, a kind of each cell of cell culture well culture, such two kinds of cells life It lives in the culture medium of same flowing, can analyze the interaction and influence between two kinds of cells of observation.Preformed hole was set both Can use its contour structures culture foundation trench in formed culture medium special flow passage, can also in preformed hole static culture it is thin Born of the same parents, or the controlled observation area as cell culture.

Further, the culture foundation trench of the tissue culture plate is rounded is distributed on supporting plate, the first driving device It is installed on inside support base, the support base is set to above supporting plate by support column, first drive shaft one end connection the One driving device, the other end of the first drive shaft are provided with first gear, and the first gear is bevel gear, the connecting rod One end is provided with bevel pinion, and the bevel pinion is engaged with the first gear, and the other end of connecting rod runs through corresponding training It supports foundation trench side wall and end is provided with impeller, have a cell culture well and a preformed hole in the culture foundation trench.Culture medium The rounded distribution of slot, is adapted to more experimental occasions, while the quantity for cultivating foundation trench experimental can need to increase Or reduce, the quantity of the connecting rod with bevel pinion need to be only increased or decreased simultaneously.Using gear transmission mode, both may be used To realize the deflecting of driving force, while having the characteristics that stable transmission, high-efficient, compact-sized again.

Further, the cell culture well sidewall symmetry of the tissue culture plate is provided with through-hole, each cell culture well An internal mating dismountable circular sleeve.The cell culture well sidewall symmetry of the tissue culture plate is provided with through-hole, often A mating dismountable circular sleeve, the height of sleeve are slightly above cell culture well inside a cell culture well, are convenient for from thin It is taken out in born of the same parents' culture hole.The cell culture well is suitble to cultivate attached cell, tests initial stage, sleeve is first put into cell culture The through-hole in hole, such cell culture well side wall is blocked, and the culture medium in cell culture well is in closing stationary state, and cell can Carried out in cell culture well internal backplane it is adherent, after cell completely it is adherent after, take away sleeve, the training in such cell culture well Culture medium in feeding base and culture foundation trench, which can converge, to be integrated.

Further, the connecting rod of the tissue culture plate is provided with rubber seal through the engaging portion of culture medium groove sidewall Circle, in this way in connecting rod rotation process, can both reduce the abrasion of culture medium groove sidewall Yu connecting rod junction, elimination is made an uproar Sound, and sealing function can be played, prevent culture medium from leaking into outside, while also can be used as a fulcrum of connecting rod, it is ensured that The dynamic balancing of connecting rod.

Further, the impeller can choose unshrouded impeller, semi-open type or double shrouded wheel, the preferred enclosed leaf of the present invention Wheel, because double shrouded wheel efficiency is higher, while can prevent culture solution from splashing around, it is ensured that culture medium is put down under impeller effect Current stabilization is dynamic.

Further, the position of the peak contact of the lever and second gear of the oscillation device is claw-type structure, claw type The bottom end of structure is connect with lever, the top of claw-type structure and the peak contact of second gear.

Further, the both ends of the lever of the oscillation device are flexibly connected by pivot structure with oscillation device ontology, The pivot structure includes the first support shaft and the second support shaft, and one that lever is connect with claw-type structure is arranged in the first support shaft End, the second support shaft are arranged in the other end of lever, and the middle part of claw-type structure is run through in one end of first support shaft, and first The other end of support axis is connected on oscillation device ontology, and lever is run through at the middle part of the second support shaft, the both ends of the second support shaft with Oscillation device ontology is fixedly connected.

Further, the oscillation device further includes two gag lever posts, and two gag lever posts are separately positioned under claw-type structure One end of the two sides of the lever in portion, the gag lever post is fixedly connected with oscillation device ontology, and the other end of gag lever post is hanging.

Further, the culture foundation trench on the tissue culture plate, cell culture well, preformed hole shape can be made Arbitrary shape meets the requirement of dynamic cultivation cell as long as being able to achieve technical effect of the invention.

Further, to prevent cell sample contaminated, the dynamic cell culture plate can a mating transparent housing, The material of transparent housing can select the materials such as plastics, organic glass to be made, and not only convenient for observation cell culture state, but also can incite somebody to action Cell sample is isolated from the outside, and prevents cell sample contaminated.The shape of transparent housing can be made arbitrary shape, as long as can incite somebody to action Cell sample on the tissue culture plate is isolated just with external environment, is not repeated it in the present invention.

In some embodiments, the first driving device is electric machine, and the electric machine is fixed with support base Connection, the side of electric machine connect the first drive shaft, setting battery case and power supply line inside the support base, power supply line connection Motor and battery case use battery to provide power supply for motor, the first power switch are arranged outside support base.Using motor driven, Structure is simple, small in size, easy to use, runs lasting stability.

In some embodiments, the first driving device is clockwork, and the clockwork is fixed with support base The side of connection, clockwork connects the first drive shaft, and the other side of clockwork is provided with the first clockwork spring handle.Using clockwork spring Driving is not necessarily to energy consumption, and structure is simple, easy to use.

In some embodiments, second driving device is electric machine, and the electric machine setting is filled in oscillation Body interior is set, second drive shaft one end is connected to motor, and the other end and the second gear center of the second drive shaft are fixed Connection, the oscillation device body interior are provided with battery case, provide power supply for motor, body exterior setting second source is opened It closes.Using motor driven, structure is simple, small in size, easy to use, runs lasting stability.

In some embodiments, second driving device is clockwork, and the clockwork setting is filled in oscillation Body interior is set, the second clockwork spring handle is arranged in the side of clockwork, after the second clockwork spring handle runs through this body sidewall of oscillation device It is exposed to that oscillation device sheet is external, the other side of clockwork is fixedly connected by the second drive shaft with second gear center.Using Clockwork spring driving, is not necessarily to energy consumption, structure is simple, easy to use.

Dynamic cell culture device disclosed by the invention when in use, starts first driving device, by driving with first First drive shaft of device connection drives first gear rotation, and first gear drives connecting rod again and connects the impeller rotation of boom end Turn, under impeller effect, culture medium can flow in culture foundation trench, and the cell in such cell hole is at dynamic environment In, then start the second driving device, the second driving device drives second gear rotation by the second drive shaft, and second gear turns During dynamic, the tooth top of second gear is contacted with the claw-type structure of lever one end, pushes claw-type structure to generate displacement, to make thick stick Bar drives pallet to swing；When second gear continues to rotate, the tooth top of second gear and the claw-type structure of lever end are disengaged, claw type Structural regression is in situ, and under effect of inertia, claw-type structure will continue to generate displacement round about, and lever drives pallet also can Continue to swing to the opposite direction of swaying direction before, in this way, the movement of lever and pallet is similar to pendulum movement principle, so Circulation, that is, realize pallet and swing, and plays oscillation action to the tissue culture plate being placed on pallet, realizes cell dynamic Culture.

The description of " first ", " second " referred to above, is used for description purposes only, is not understood to indicate or imply Its relative importance or the quantity for implicitly indicating indicated technical characteristic, define the feature of " first ", " second " as a result, It can explicitly or implicitly include at least one of the features.

Compared with prior art, the beneficial effect of technical solution of the present invention is:

1. small in size: being easily put into the instruments such as cell incubator and super-clean bench and use；

2. easy to use: it is easy to operate, it is not required to connect excessive equipment and AC power source；

3. simulating dynamic environment culture cell in animal body, experimental result accuracy and science can be improved.

Detailed description of the invention

To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other Attached drawing.

Fig. 1 is the schematic perspective view of embodiment 1；

Fig. 2 is the schematic perspective view of embodiment 2；

Fig. 3 is the schematic perspective view of embodiment 3；

Fig. 4 is the schematic perspective view of embodiment 4；

Fig. 5 is the schematic perspective view of embodiment 5；

Fig. 6 is the schematic perspective view of embodiment 6；

Fig. 7 is the schematic perspective view of embodiment 7；

Fig. 8 is the schematic perspective view of embodiment 8；

Fig. 9 is the schematic perspective view of embodiment 9；

Figure 10 is the schematic perspective view of embodiment 10；

Figure 11 is the schematic perspective view of embodiment 11；

Figure 12 is the schematic perspective view of embodiment 12；

Figure 13 is motor driven oscillation device schematic perspective view；

Figure 14 is the A-A sectional view of Figure 13；

Figure 15 is clockwork spring driving oscillation device schematic perspective view；

Figure 16 is the B-B sectional view of Figure 15；

Figure 17 is the attachment structure schematic diagram of second gear and claw-type structure；

Figure 18 is the connection structure side view of first gear (bevel gear wheel) and bevel pinion 152；

Figure 19 is the connection structure bottom view of first gear (bevel gear wheel) and bevel pinion 152；

Figure 20 is the structural schematic diagram of cell culture well and sleeve；

Figure 21 is the schematic perspective view of impeller；

Figure 22 is the A-A sectional view of Figure 21.

In figure: 11- supporting plate, 12- cell culture well, 121- sleeve, 122- through-hole, 13- first driving device, 131- first Drive shaft, 132- support rod, 133- support base, 134- support column, 14- first gear, 141- external screw thread, 15- connecting rod, 151- Impeller, 152- bevel pinion, 16- culture foundation trench, 161- preformed hole, 17- rubber seal, the first clockwork spring of 18- handle, 19- the One power switch；

21- oscillation device ontology, 22- pallet, 221- pillar, the second driving device of 23-, the second drive shaft of 231-, the second tooth of 24- Wheel, 241- claw-type structure, 25- lever, the first support shaft of 251-, the second support shaft of 252-, 26- gag lever post, 27- the second clockwork spring hand Handle, 28- second source switch, 29- battery case；

3- clamping unit.

Specific embodiment

The attached figures are only used for illustrative purposes and cannot be understood as limitating the patent；In order to which the present embodiment is better described, The certain components of attached drawing have omission, zoom in or out, and do not represent the size of actual product；Those skilled in the art are come It says, the omitting of some known structures and their instructions in the attached drawings are understandable.

The description of " first ", " second ", is used for description purposes only involved in embodiment of the present invention, and cannot understand For its relative importance of indication or suggestion or implicitly indicate the quantity of indicated technical characteristic, define as a result, " first ", The feature of " second " can explicitly or implicitly include at least one of the features.

In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation " " connects Connect " it shall be understood in a broad sense, for example, it may be being fixedly connected, it may be a detachable connection, or be integrally connected；It can be machine Tool connection, is also possible to be electrically connected；It can be directly connected, be also possible to be indirectly connected with by intermediary, it may be said that two Connection inside element.For the ordinary skill in the art, above-mentioned term can be understood at this as the case may be The concrete meaning of invention.

For a clearer understanding of the technical characteristics, objects and effects of the present invention, now control attached drawing is described in detail A specific embodiment of the invention, embodiments of the present invention are not limited thereto.

Embodiment 1:

As shown in Fig. 1, Figure 13 and Figure 14, a kind of dynamic cell culture device, including tissue culture plate and oscillation device:

The tissue culture plate includes supporting plate 11, cell culture well 12, first driving device 13, support base 133, the first drive shaft 131, first gear 14, connecting rod 15, culture foundation trench 16, preformed hole 161 and impeller 151, the first driving device 13 are set It is placed in 133 top of support base, the support base 133 is set on supporting plate 11, first drive shaft 131 and first driving device 13 connections, the first driving device 13 drive first gear 14 and connecting rod 15 to rotate by the first drive shaft 131, the company Extension bar 15 is through culture 16 side wall of foundation trench and 15 end of connecting rod is provided with impeller 151, and the culture foundation trench 16 is internally provided with Cell culture well 12 and preformed hole 161；

The oscillation device includes including oscillation device ontology 21, pallet 22, the second driving device 23, the second drive shaft 231, the Two gears 24 and lever 25, second driving device 23 are arranged inside oscillation device ontology 21, second drive shaft 231 one end are connect with the second driving device 23, and the other end of the second drive shaft 231 is connect with 24 center of second gear, and described The tooth top of two gears 24 is contacted with the lever 25, and the both ends of the lever 25 are flexibly connected with oscillation device ontology 21, described Pallet 22 is connected to the middle part of lever 25 by pillar 221；

Baffle is surrounded by the pallet 22 of the oscillation device, the supporting plate 11 of the tissue culture plate is nested in the pallet 22 In the cavity that upper surrounding baffle is encircled into, clamping unit 3, the clamping unit 3 are provided between the pallet 22 and supporting plate 11 To keep off needle, one end of the gear needle is flexibly connected with baffle, and the other end for keeping off needle can be stirred, and supporting plate 11 can be pressed on pallet On 22, the clamping unit 3 is provided with multiple between supporting plate 11 and pallet 22.

The culture foundation trench 16 of the tissue culture plate, which is square, to be symmetrically distributed on supporting plate 11, first drive shaft 131 One end is connect with first driving device 13, the other end of the first drive shaft 131 and 132 activity of support rod being arranged on supporting plate 11 Connection, 131 surface spaced set of the first drive shaft have external screw thread 141, and the external screw thread 141 is set with fixation in the middle part of connecting rod 15 The first gear 14 set engages, and the both ends of the connecting rod 15 extend through corresponding 16 side wall of culture foundation trench and connecting rod 15 Both ends be provided with impeller 151.Culture foundation trench 16 is square distribution, is adapted to more experimental occasions.

A cell culture well 12 and a preformed hole 161 are provided in the culture foundation trench 16.Cell culture well 12 is used Cultivate attached cell, setting preformed hole 161 both can use the spy that its contour structures forms culture medium in culture foundation trench 16 Different runner, can also in preformed hole 161 static culture cell, or the controlled observation area as cell culture.

The connecting rod 15 of the tissue culture plate is provided with rubber seal 17 through the engaging portion of culture 16 side wall of foundation trench, In this way in 15 rotation process of connecting rod, the abrasion of culture 16 side wall of foundation trench and 15 junction of connecting rod can be both reduced, is eliminated Noise, and sealing function can be played, prevent culture medium from leaking into outside, while also can be used as a fulcrum of connecting rod 15, Ensure the dynamic balancing of connecting rod 15.

As shown in figure 20,12 sidewall symmetry of cell culture well of the tissue culture plate is provided with through-hole 122, each cell The 12 mating dismountable circular sleeve 121 in inside of culture hole, the height of sleeve 121 are slightly above cell culture well 12, are convenient for It is taken out out of cell culture well 12.The cell culture well 12 is suitble to culture attached cell, tests initial stage, first puts sleeve 121 Enter cell culture well 12, the through-hole 122 of such 12 side wall of cell culture well is blocked, and the culture medium in cell culture well 12 is in Close stationary state, cell can be carried out in 12 internal backplane of cell culture well it is adherent, after cell completely it is adherent after, take away sleeve 121, the culture medium in culture medium and culture foundation trench 16 in such cell culture well 12, which can converge, to be integrated.

As shown in figure 21 and figure, the preferred double shrouded wheel of the impeller 151, because double shrouded wheel efficiency is higher, while can To prevent culture solution from splashing around, it is ensured that culture medium smooth flow under the effect of impeller 151.

As shown in Figure 13, Figure 14 and Figure 17, the portion of the peak contact of the lever 25 and second gear 24 of the oscillation device Position is claw-type structure 241, and the bottom end of claw-type structure 241 is connect with lever 25, top and the second gear 24 of claw-type structure 241 Peak contact.

The both ends of the lever 25 of the oscillation device are flexibly connected by pivot structure with oscillation device ontology 21, the pivot Axle construction includes the first support shaft 251 and the second support shaft 252, and the first support shaft 251 is arranged in lever 25 and claw-type structure 241 The other end of lever 25 is arranged in one end of connection, the second support shaft 252, and claw type is run through in one end of first support shaft 251 The other end at the middle part of structure 241, the first support shaft 251 is connected on oscillation device ontology 21, the middle part of the second support shaft 252 Through lever 25, the both ends of the second support shaft 252 are fixedly connected with oscillation device ontology 21.

The oscillation device further includes two gag lever posts 26, and two gag lever posts 26 are separately positioned on 241 lower part of claw-type structure Lever 25 two sides, one end of the gag lever post 26 is fixedly connected with oscillation device ontology 21, and the other end of gag lever post 26 is outstanding It is empty.

As shown in Figure 1, the first driving device 13 is electric machine, the first driving of side connection of the electric machine Axis 131.Battery is used to provide power supply for motor, electric machine bottom is fixedly connected with support base 133, inside the support base 133 Battery case and power supply line are set, and power supply line connects motor and battery case, the first power switch 19 is arranged outside support base 133.It adopts It being driven with electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

As shown in figure 14, second driving device 23 is electric machine, and the electric machine is arranged in oscillation device sheet Inside body 21, described second drive shaft, 231 one end is connect with electric machine, the other end and second gear of the second drive shaft 231 24 centers are fixedly connected, and the oscillation device ontology 21 is internally provided with battery case, provide power supply, body exterior setting for motor Second source switch 28.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

Dynamic cell culture device disclosed by the invention when in use, starts first driving device 13, by driving with first The first drive shaft 131 that dynamic device 13 connects drives first gear 14 to rotate, and first gear 14 drives connection by external screw thread 141 The impeller 151 of 15 end of bar 15 and connecting rod rotates, and under the effect of impeller 151, culture medium can flow in culture foundation trench 16 Come, the cell in such cell hole is in dynamic environment, then starts the second driving device 23, and the second driving device 23 is logical Crossing the second drive shaft 231 drives second gear 24 to rotate, in 24 rotation process of second gear, the tooth top and lever of second gear 24 The claw-type structure 241 of 25 one end contacts, and claw-type structure 241 is pushed to generate displacement, to make lever 25 that pallet 22 be driven to swing；When Second gear 24 continues to rotate, and the tooth top of second gear 24 and the claw-type structure 241 of 25 end of lever disengage, claw-type structure 241 It keeps in the center, under effect of inertia, claw-type structure 241 will continue to generate displacement round about, and lever 25 drives pallet 22 Also can continue to swing to the opposite direction of swaying direction before, in this way, the movement of lever 25 and pallet 22 is similar to simple harmonic motion Principle so recycles, that is, realizes pallet 22 and swing, and plays oscillation to the tissue culture plate being placed on pallet 22 and makees With realization cell dynamic cultivation.

Embodiment 2:

As depicted in figs. 1 and 2, the difference of embodiment 2 and embodiment 1 is the driving method of second driving device 23 It is different.

As shown in Fig. 2 and Figure 16, the first driving device 13 is electric machine, the side connection the of the electric machine One drive shaft 131.Battery is used to provide power supply for motor, electric machine bottom is fixedly connected with support base 133, the support base 133 inside setting battery cases and power supply line, power supply line connect motor and battery case, the first power supply are arranged outside support base 133 and opens Close 19.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

Second driving device 23 is clockwork, and the clockwork is arranged inside oscillation device ontology 21, hair The second clockwork spring handle 27 is arranged in the side of strip device, and the second clockwork spring handle 27 is exposed to oscillation after running through 21 side wall of oscillation device ontology Outside device noumenon 21, the other side of clockwork is fixedly connected by the second drive shaft 231 with 24 center of second gear.Using hair Strip device driving, is not necessarily to energy consumption, structure is simple, easy to use.

Embodiment 3:

As shown in figures 1 and 3, the difference of embodiment 3 and embodiment 1 is the driving method of the first driving device 13 It is different.

As shown in Fig. 3 and Figure 14, the first driving device 13 is clockwork, the side connection the of the clockwork One drive shaft 131, the other side of clockwork are provided with the first clockwork spring handle 18.It is driven using clockwork, is not necessarily to energy consumption, knot Structure is simple, easy to use.

Second driving device 23 is electric machine, and the electric machine is arranged inside oscillation device ontology 21, institute 231 one end of the second drive shaft to be stated to be connected to motor, the other end of the second drive shaft 231 is fixedly connected with 24 center of second gear, The oscillation device ontology 21 is internally provided with battery case 29, provides power supply for motor, body exterior is arranged second source and switchs 28.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

Embodiment 4:

As shown in Figure 1 and Figure 4, the difference of embodiment 4 and embodiment 1 is the first driving device 13 and the second driving The driving method of device 23 is different.

As shown in Fig. 4 and Figure 16, the first driving device 13 is clockwork, the side connection the of the clockwork One drive shaft 131, the other side of clockwork are provided with the first clockwork spring handle 18.It is driven using clockwork, is not necessarily to energy consumption, knot Structure is simple, easy to use.

Second driving device 23 is clockwork, and the clockwork is arranged inside oscillation device ontology 21, hair The second clockwork spring handle 27 is arranged in the side of strip device, and the second clockwork spring handle 27 is exposed to oscillation after running through 21 side wall of oscillation device ontology Outside device noumenon 21, the other side of clockwork is fixedly connected by the second drive shaft 231 with 24 center of second gear.Using hair Strip device driving, is not necessarily to energy consumption, structure is simple, easy to use.

Embodiment 5:

As shown in Fig. 5, Figure 13 and Figure 14, a kind of dynamic cell culture device, including tissue culture plate and oscillation device:

The tissue culture plate includes supporting plate 11, cell culture well 12, first driving device 13, support base 133, the first drive shaft 131, first gear 14, connecting rod 15, culture foundation trench 16, preformed hole 161 and impeller 151, the first driving device 13 are set It is placed in 133 top of support base, the support base 133 is set on supporting plate 11, first drive shaft 131 and first driving device 13 connections, the first driving device 13 drive first gear 14 and connecting rod 15 to rotate by the first drive shaft 131, the company Extension bar 15 is through culture 16 side wall of foundation trench and 15 end of connecting rod is provided with impeller 151, and the culture foundation trench 16 is internally provided with Cell culture well 12 and preformed hole 161；

The oscillation device includes including oscillation device ontology 21, pallet 22, the second driving device 23, the second drive shaft 231, the Two gears 24 and lever 25, second driving device 23 are arranged inside oscillation device ontology 21, second drive shaft 231 one end are connect with the second driving device 23, and the other end of the second drive shaft 231 is connect with 24 center of second gear, and described The tooth top of two gears 24 is contacted with the lever 25, and the both ends of the lever 25 are flexibly connected with oscillation device ontology 21, described Pallet 22 is connected to the middle part of lever 25 by pillar 221；

Baffle is surrounded by the pallet 22 of the oscillation device, the supporting plate 11 of the tissue culture plate is nested in the pallet 22 In the cavity that upper surrounding baffle is encircled into, clamping unit 3, the clamping unit 3 are provided between the pallet 22 and supporting plate 11 To keep off needle, one end of the gear needle is flexibly connected with baffle, and the other end for keeping off needle can be stirred, and supporting plate 11 can be pressed on pallet On 22, the clamping unit 3 is provided with multiple between supporting plate 11 and pallet 22.

The culture foundation trench 16 of the tissue culture plate, which is square, to be symmetrically distributed on supporting plate 11, first drive shaft 131 One end is connect with first driving device 13, the other end of the first drive shaft 131 and 132 activity of support rod being arranged on supporting plate 11 Connection, 131 surface spaced set of the first drive shaft have external screw thread 141, and the external screw thread 141 is set with fixation in the middle part of connecting rod 15 The first gear 14 set engages, and the both ends of the connecting rod 15 extend through corresponding 16 side wall of culture foundation trench and connecting rod 15 Both ends be provided with impeller 151.Culture foundation trench 16 is square distribution, is adapted to more experimental occasions.

There are two cell culture well 12 and a preformed holes 161 for setting in the culture foundation trench 16.Two cell trainings are set The purpose for supporting hole 12 is for cultivating two kinds of cells, and each cell culture well 12 cultivates a kind of cell, such two kinds of cell lifes In the culture medium of same flowing, the interaction and influence between two kinds of cells of observation can analyze.Preformed hole 161 was set both It can use the special flow passage that its contour structures forms culture medium in culture foundation trench 16, it can also be static in preformed hole 161 Cultivate cell, or the controlled observation area as cell culture.

The connecting rod 15 of the tissue culture plate is provided with rubber seal 17 through the engaging portion of culture 16 side wall of foundation trench, In this way in 15 rotation process of connecting rod, the abrasion of culture 16 side wall of foundation trench and 15 junction of connecting rod can be both reduced, is eliminated Noise, and sealing function can be played, prevent culture medium from leaking into outside, while also can be used as a fulcrum of connecting rod 15, Ensure the dynamic balancing of connecting rod 15.

As shown in figure 20,12 sidewall symmetry of cell culture well of the tissue culture plate is provided with through-hole 122, each cell The 12 mating dismountable circular sleeve 121 in inside of culture hole, the height of sleeve 121 are slightly above cell culture well 12, are convenient for It is taken out out of cell culture well 12.The cell culture well 12 is suitble to culture attached cell, tests initial stage, first puts sleeve 121 Enter cell culture well 12, the through-hole 122 of such 12 side wall of cell culture well is blocked, and the culture medium in cell culture well 12 is in Close stationary state, cell can be carried out in 12 internal backplane of cell culture well it is adherent, after cell completely it is adherent after, take away sleeve 121, the culture medium in culture medium and culture foundation trench 16 in such cell culture well 12, which can converge, to be integrated.

As shown in figure 21 and figure, the preferred double shrouded wheel of the impeller 151, because double shrouded wheel efficiency is higher, while can To prevent culture solution from splashing around, it is ensured that culture medium smooth flow under the effect of impeller 151.

As shown in Figure 13, Figure 14 and Figure 17, the portion of the peak contact of the lever 25 and second gear 24 of the oscillation device Position is claw-type structure 241, and the bottom end of claw-type structure 241 is connect with lever 25, top and the second gear 24 of claw-type structure 241 Peak contact.

The both ends of the lever 25 of the oscillation device are flexibly connected by pivot structure with oscillation device ontology 21, the pivot Axle construction includes the first support shaft 251 and the second support shaft 252, and the first support shaft 251 is arranged in lever 25 and claw-type structure 241 The other end of lever 25 is arranged in one end of connection, the second support shaft 252, and claw type is run through in one end of first support shaft 251 The other end at the middle part of structure 241, the first support shaft 251 is connected on oscillation device ontology 21, the middle part of the second support shaft 252 Through lever 25, the both ends of the second support shaft 252 are fixedly connected with oscillation device ontology 21.

Further, the oscillation device further includes two gag lever posts 26, and two gag lever posts 26 are separately positioned on claw type knot One end of the two sides of the lever 25 of 241 lower part of structure, the gag lever post 26 is fixedly connected with oscillation device ontology 21, gag lever post 26 The other end is hanging.

As shown in figure 5, the first driving device 13 is electric machine, the first driving of side connection of the electric machine Axis 131.Battery is used to provide power supply for motor, electric machine bottom is fixedly connected with support base 133, inside the support base 133 Battery case and power supply line are set, and power supply line connects motor and battery case, the first power switch 19 is arranged outside support base 133.It adopts It being driven with electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

As shown in figure 14, second driving device 23 is electric machine, and the electric machine is arranged in oscillation device sheet Inside body 21, described second drive shaft, 231 one end is connected to motor, in the other end and second gear 24 of the second drive shaft 231 The heart is fixedly connected, and the oscillation device ontology 21 is internally provided with battery case 29, provides power supply for motor, body exterior setting the Two power switches 28.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

Dynamic cell culture device disclosed by the invention when in use, starts first driving device 13, by driving with first The first drive shaft 131 that dynamic device 13 connects drives first gear 14 to rotate, and first gear 14 drives connection by external screw thread 141 The impeller 151 of 15 end of bar 15 and connecting rod rotates, and under the effect of impeller 151, culture medium can flow in culture foundation trench 16 Come, the cell in such cell hole is in dynamic environment, then starts the second driving device 23, and the second driving device 23 is logical Crossing the second drive shaft 231 drives second gear 24 to rotate, in 24 rotation process of second gear, the tooth top and lever of second gear 24 The claw-type structure 241 of 25 one end contacts, and claw-type structure 241 is pushed to generate displacement, to make lever 25 that pallet 22 be driven to swing；When Second gear 24 continues to rotate, and the tooth top of second gear 24 and the claw-type structure 241 of 25 end of lever disengage, claw-type structure 241 It keeps in the center, under effect of inertia, claw-type structure 241 will continue to generate displacement round about, and lever 25 drives pallet 22 Also can continue to swing to the opposite direction of swaying direction before, in this way, the movement of lever 25 and pallet 22 is similar to simple harmonic motion Principle so recycles, that is, realizes pallet 22 and swing, and plays oscillation to the tissue culture plate being placed on pallet 22 and makees With realization cell dynamic cultivation.

Embodiment 6:

As shown in Figure 5 and Figure 6, the difference of embodiment 6 and embodiment 5 is the driving method of second driving device 23 It is different.

As shown in Fig. 6 and Figure 16, the first driving device 13 is electric machine, the side connection the of the electric machine One drive shaft 131.Battery is used to provide power supply for motor, electric machine bottom is fixedly connected with support base 133, the support base 133 inside setting battery cases and power supply line, power supply line connect motor and battery case, the first power supply are arranged outside support base 133 and opens Close 19.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

Second driving device 23 is clockwork, and the clockwork is arranged inside oscillation device ontology 21, hair The second clockwork spring handle 27 is arranged in the side of strip device, and the second clockwork spring handle 27 is exposed to oscillation after running through 21 side wall of oscillation device ontology Outside device noumenon 21, the other side of clockwork is fixedly connected by the second drive shaft 231 with 24 center of second gear.Using hair Strip device driving, is not necessarily to energy consumption, structure is simple, easy to use.

Embodiment 7:

As shown in figure 5 and figure 7, the difference of embodiment 7 and embodiment 5 is the driving method of the first driving device 13 It is different.

As shown in Fig. 7 and Figure 14, the first driving device 13 is clockwork, the side connection the of the clockwork One drive shaft 131, the other side of clockwork are provided with the first clockwork spring handle 18.It is driven using clockwork, is not necessarily to energy consumption, knot Structure is simple, easy to use.

Second driving device 23 is electric machine, and the electric machine is arranged inside oscillation device ontology 21, institute It states 231 one end of the second drive shaft to connect with electric machine, the fixed company of the other end of the second drive shaft 231 and 24 center of second gear It connects, the oscillation device ontology 21 is internally provided with battery case 29, provides power supply for motor, body exterior setting second source is opened Close 28.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

Embodiment 8:

As shown in Figure 5 and Figure 8, the difference of embodiment 8 and embodiment 5 is the first driving device 13 and the second driving The driving method of device 23 is different.

As shown in Fig. 8 and Figure 16, the first driving device 13 is clockwork, the side connection the of the clockwork One drive shaft 131, the other side of clockwork are provided with the first clockwork spring handle 18.It is driven using clockwork, is not necessarily to energy consumption, knot Structure is simple, easy to use.

Second driving device 23 is clockwork, and the clockwork is arranged inside oscillation device ontology 21, hair The second clockwork spring handle 27 is arranged in the side of strip device, and the second clockwork spring handle 27 is exposed to oscillation after running through 21 side wall of oscillation device ontology Outside device noumenon 21, the other side of clockwork is fixedly connected by the second drive shaft 231 with 24 center of second gear.Using hair Strip device driving, is not necessarily to energy consumption, structure is simple, easy to use.

Embodiment 9:

As shown in Fig. 9, Figure 13 and Figure 14, a kind of dynamic cell culture device, including tissue culture plate and oscillation device:

The tissue culture plate includes supporting plate 11, cell culture well 12, first driving device 13, support base 133, the first drive shaft 131, first gear 14, connecting rod 15, culture foundation trench 16, preformed hole 161 and impeller 151, the first driving device 13 are set It sets inside support base 133, the support base 133 is set to 11 top of supporting plate, first drive shaft by support column 134 131 connect with first driving device 13, and the first driving device 13 drives first gear 14 by the first drive shaft 131 and connects Extension bar 15 rotates, and the connecting rod 15 is through culture 16 side wall of foundation trench and 15 end of connecting rod is provided with impeller 151, the culture Foundation trench 16 is internally provided with cell culture well 12 and preformed hole 161；

The oscillation device includes including oscillation device ontology 21, pallet 22, the second driving device 23, the second drive shaft 231, the Two gears 24 and lever 25, second driving device 23 are arranged inside oscillation device ontology 21, second drive shaft 231 one end are connect with the second driving device 23, and the other end of the second drive shaft 231 is connect with 24 center of second gear, and described The tooth top of two gears 24 is contacted with the lever 25, and the both ends of the lever 25 are flexibly connected with oscillation device ontology 21, described Pallet 22 is connected to the middle part of lever 25 by pillar 221；

Baffle is surrounded by the pallet 22 of the oscillation device, the supporting plate 11 of the tissue culture plate is nested in the pallet 22 In the cavity that upper surrounding baffle is encircled into, clamping unit 3, the clamping unit 3 are provided between the pallet 22 and supporting plate 11 To keep off needle, one end of the gear needle is flexibly connected with baffle, and the other end for keeping off needle can be stirred, and supporting plate 11 can be pressed on pallet On 22, the clamping unit 3 is provided with multiple between supporting plate 11 and pallet 22.

As shown in Fig. 9, Figure 18 and Figure 19, the culture foundation trench 16 of the tissue culture plate is rounded to be distributed on supporting plate 11, There are a cell culture well 12 and a preformed hole 161 in the culture foundation trench 16, the first driving device 13 is set to branch It supports inside seat 133, the support base 133 is set to 11 top of supporting plate, described first drive shaft, 131 one end by support column 134 First driving device 13 is connected, the other end of the first drive shaft 131 is provided with first gear 14, and the first gear 14 is auger Gear, one end of the connecting rod 15 are provided with bevel pinion 152, the bevel pinion 152 and the first gear 14(auger Gear) engagement, the other end of connecting rod 15 is through corresponding 16 side wall of culture foundation trench and end is provided with impeller 151.

The connecting rod 15 of the tissue culture plate is provided with rubber seal 17 through the engaging portion of culture 16 side wall of foundation trench, In this way in 15 rotation process of connecting rod, the abrasion of culture 16 side wall of foundation trench and 15 junction of connecting rod can be both reduced, is eliminated Noise, and sealing function can be played, prevent culture medium from leaking into outside, while also can be used as a fulcrum of connecting rod 15, Ensure the dynamic balancing of connecting rod 15.

As shown in figure 20,12 sidewall symmetry of cell culture well of the tissue culture plate is provided with through-hole 122, each cell The 12 mating dismountable circular sleeve 121 in inside of culture hole, the height of sleeve 121 are slightly above cell culture well 12, are convenient for It is taken out out of cell culture well 12.The cell culture well 12 is suitble to culture attached cell, tests initial stage, first puts sleeve 121 Enter cell culture well 12, the through-hole 122 of such 12 side wall of cell culture well is blocked, and the culture medium in cell culture well 12 is in Close stationary state, cell can be carried out in 12 internal backplane of cell culture well it is adherent, after cell completely it is adherent after, take away sleeve 121, the culture medium in culture medium and culture foundation trench 16 in such cell culture well 12, which can converge, to be integrated.

As shown in figure 21 and figure, the impeller 151 is double shrouded wheel, because double shrouded wheel efficiency is higher, while can be with Prevent culture solution from splashing around, it is ensured that culture medium smooth flow under the effect of impeller 151.

As shown in Figure 13, Figure 14 and Figure 17, the portion of the peak contact of the lever 25 and second gear 24 of the oscillation device Position is claw-type structure 241, and the bottom end of claw-type structure 241 is connect with lever 25, top and the second gear 24 of claw-type structure 241 Peak contact.

The both ends of the lever 25 of the oscillation device are flexibly connected by pivot structure with oscillation device ontology 21, the pivot Axle construction includes the first support shaft 251 and the second support shaft 252, and the first support shaft 251 is arranged in lever 25 and claw-type structure 241 The other end of lever 25 is arranged in one end of connection, the second support shaft 252, and claw type is run through in one end of first support shaft 251 The other end at the middle part of structure 241, the first support shaft 251 is connected on oscillation device ontology 21, the middle part of the second support shaft 252 Through lever 25, the both ends of the second support shaft 252 are fixedly connected with oscillation device ontology 21.

The oscillation device further includes two gag lever posts 26, and two gag lever posts 26 are separately positioned on 241 lower part of claw-type structure Lever 25 two sides, one end of the gag lever post 26 is fixedly connected with oscillation device ontology 21, and the other end of gag lever post 26 is outstanding It is empty.

As shown in figure 9, the first driving device is electric machine, the electric machine is installed in support base 133, institute The first drive shaft 131 is stated to connect through 133 bottom of support base with electric machine.Inside the support base 133 be arranged battery case and Power supply line uses battery to provide power supply for motor, and power supply line connects motor and battery case, the first electricity of setting outside support base 133 Source switch 19.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

As shown in figure 14, second driving device 23 is electric machine, and the electric machine is arranged in oscillation device sheet Inside body 21, described second drive shaft, 231 one end is connect with electric machine, the other end and second gear of the second drive shaft 231 24 centers are fixedly connected, and the oscillation device ontology 21 is internally provided with battery case 29, provide power supply for motor, body exterior is set Set second source switch 28.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

Dynamic cell culture device disclosed by the invention when in use, starts first driving device 13, by driving with first The first drive shaft 131 that dynamic device 13 connects drives first gear 14(bevel gear wheel) rotation, first gear 14(bevel gear wheel) The impeller 151 of connecting rod 15 and 15 end of connecting rod is driven to rotate by bevel pinion 152, under the effect of impeller 151, culture medium It can flow in culture foundation trench 16, the cell in such cell hole is in dynamic environment, then starts the second driving Device 23, the second driving device 23 drive second gear 24 to rotate by the second drive shaft 231,24 rotation process of second gear In, the tooth top of second gear 24 is contacted with the claw-type structure 241 of 25 one end of lever, and push claw-type structure 241 to generate displacement, thus Make lever 25 that pallet 22 be driven to swing；When second gear 24 continues to rotate, the tooth top of second gear 24 and the pawl of 25 end of lever Shape structure 241 disengages, and claw-type structure 241 is kept in the center, and under effect of inertia, claw-type structure 241 will continue to produce round about Raw displacement, and lever 25 drives pallet 22 also can continue to swing to the opposite direction of swaying direction before, in this way, lever 25 and support The movement of disk 22 is similar to pendulum movement principle, so recycles, that is, realizes pallet 22 and swing, to being placed on pallet 22 Tissue culture plate play oscillation action, realize cell dynamic cultivation.

Embodiment 10:

As shown in Figure 9 and Figure 10, the difference of embodiment 10 and embodiment 9 is the driving side of second driving device 23 Formula is different.

As shown in Figure 10 and Figure 16, the first driving device is electric machine, and the electric machine is installed on support base In 133, first drive shaft 131 is connect through 133 bottom of support base with electric machine.It is arranged inside the support base 133 Battery case and power supply line, use battery to provide power supply for motor, and power supply line connects motor and battery case, sets outside support base 133 Set the first power switch 19.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

Second driving device 23 is clockwork, and the clockwork is arranged inside oscillation device ontology 21, hair The second clockwork spring handle 27 is arranged in the side of strip device, and the second clockwork spring handle 27 is exposed to oscillation after running through 21 side wall of oscillation device ontology Outside device noumenon 21, the other side of clockwork is fixedly connected by the second drive shaft 231 with 24 center of second gear.Using hair Strip device driving, is not necessarily to energy consumption, structure is simple, easy to use.

Embodiment 11:

As shown in figures 9 and 11, the difference of embodiment 11 and embodiment 9 is the driving side of the first driving device 13 Formula is different.

As shown in Figure 10 and Figure 16, the first driving device is clockwork, and the clockwork is installed on support base Inside 133, first drive shaft 131 is connected through the bottom of support base 133 and the side of clockwork, first clockwork spring Handle 18 is connected through the top of support base 133 and the other side of clockwork.It is driven using clockwork, is not necessarily to energy consumption, knot Structure is simple, easy to use.

Second driving device 23 is electric machine, and the electric machine is arranged inside oscillation device ontology 21, institute It states 231 one end of the second drive shaft to connect with electric machine, the fixed company of the other end of the second drive shaft 231 and 24 center of second gear It connects, the oscillation device ontology 21 is internally provided with battery case, provides power supply for motor, body exterior is arranged second source and switchs 28.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.

Embodiment 12:

As shown in figs. 9 and 12, the difference of embodiment 10 and embodiment 9 is that the first driving device 13 and second is driven The driving method of dynamic device 23 is different.

As shown in Figure 10 and Figure 16, the first driving device is clockwork, and the clockwork is installed on support base Inside 133, first drive shaft 131 is connected through the bottom of support base 133 and the side of clockwork, first clockwork spring Handle 18 is connected through the top of support base 133 and the other side of clockwork.It is driven using clockwork, is not necessarily to energy consumption, knot Structure is simple, easy to use.

Second driving device 23 is clockwork, and the clockwork is arranged inside oscillation device ontology 21, hair The second clockwork spring handle 27 is arranged in the side of strip device, and the second clockwork spring handle 27 is exposed to oscillation after running through 21 side wall of oscillation device ontology Outside device noumenon 21, the other side of clockwork is fixedly connected by the second drive shaft 231 with 24 center of second gear.Using hair Strip device driving, is not necessarily to energy consumption, structure is simple, easy to use.

In figure, description positional relationship only for illustration, should not be understood as the limitation to this patent；Obviously, this hair Bright the above embodiment is merely an example for clearly illustrating the present invention, and is not to embodiments of the present invention It limits.For those of ordinary skill in the art, other different forms can also be made on the basis of the above description Variation or variation.There is no necessity and possibility to exhaust all the enbodiments.It is all the spirit and principles in the present invention it Any modifications, equivalent replacements, and improvements made by interior etc., should all be included in the scope of protection of the claims of the present invention.

Claims (14)

1. a kind of dynamic cell culture device, which is characterized in that the dynamic cell culture device includes tissue culture plate and vibration Swing device:

The tissue culture plate includes supporting plate, cell culture well, first driving device, support base, the first drive shaft, the first tooth Wheel, connecting rod, culture foundation trench, preformed hole and impeller, the first driving device are connect with support base, first drive shaft It is connect with first driving device, the first driving device drives first gear and connecting rod to rotate by the first drive shaft, institute It states connecting rod and the end of culture medium groove sidewall and connecting rod is provided with impeller, the culture foundation trench is internally provided with cell training Support hole and preformed hole；

The oscillation device include including oscillation device ontology, pallet, the second driving device, the second drive shaft, second gear with And lever, the second driving device setting are filled in oscillation device body interior, second drive shaft one end with the second driving Connection is set, the other end of the second drive shaft is connect with second gear center, the tooth top of the second gear and the lever contact, The both ends of the lever are flexibly connected with oscillation device ontology, and the pallet is connected to the middle part of lever by pillar；

Baffle is surrounded by the pallet of the oscillation device, the supporting plate of the tissue culture plate is nested in surrounding on the pallet In the cavity that baffle is encircled into, clamping unit is symmetrically arranged between the pallet and supporting plate.

2. dynamic cell culture device according to claim 1, which is characterized in that the clamping unit is gear needle, described One end of gear needle is flexibly connected with the baffle of pallet surrounding, and supporting plate is pressed on pallet by the other end for keeping off needle.

3. dynamic cell culture device according to claim 2, which is characterized in that the culture foundation trench of the tissue culture plate It is square and is symmetrically distributed on supporting plate, the culture foundation trench is internally provided with a cell culture well and a preformed hole, described First driving device is set to support base top, and the support base is set on supporting plate, first drive shaft one end and first Driving device connection, the other end of the first drive shaft are flexibly connected with the support rod being arranged on supporting plate, the first drive shaft surface Spaced set has external screw thread, and the external screw thread is engaged with the first gear being fixedly installed in the middle part of connecting rod, the connecting rod Both ends extend through corresponding culture medium groove sidewall and the both ends of connecting rod are provided with impeller.

4. dynamic cell culture device according to claim 2, which is characterized in that the culture foundation trench of the tissue culture plate It is square and is symmetrically distributed on supporting plate, the culture foundation trench is internally provided with two cell culture wells and a preformed hole, described First driving device is set to support base top, and the support base is set on supporting plate, first drive shaft one end and first Driving device connection, the other end of the first drive shaft are flexibly connected with the support rod being arranged on supporting plate, the first drive shaft surface Spaced set has external screw thread, and the external screw thread is engaged with the first gear being fixedly installed in the middle part of connecting rod, the connecting rod Both ends extend through corresponding culture medium groove sidewall and the both ends of connecting rod are provided with impeller.

5. dynamic cell culture device according to claim 2, which is characterized in that the culture foundation trench of the tissue culture plate Rounded to be distributed on supporting plate, the culture foundation trench is internally provided with a cell culture well and a preformed hole, and described first Driving device is set to inside support base, and the support base is set to above supporting plate by support column, first drive shaft one End connection first driving device, the other end of the first drive shaft are provided with first gear, and the first gear is bevel gear, described One end of connecting rod is provided with bevel pinion, and the bevel pinion is engaged with the first gear, and the other end of connecting rod runs through Corresponding culture medium groove sidewall and end is provided with impeller.

6. dynamic cell culture device according to claim 1-5, which is characterized in that the cell culture well side Wall is symmetrically arranged with through-hole, a mating dismountable circular sleeve inside each cell culture well.

7. dynamic cell culture device according to claim 6, which is characterized in that the connecting rod is through culture foundation trench side The engaging portion of wall is provided with rubber seal, and the impeller is double shrouded wheel.

8. dynamic cell culture device according to claim 7, which is characterized in that the lever of the oscillation device and second The position of the peak contact of gear is claw-type structure, and the bottom end of claw-type structure is connect with lever, the top of claw-type structure and second The peak contact of gear.

9. dynamic cell culture device according to claim 8, which is characterized in that the both ends of the lever pass through pivot knot Structure is flexibly connected with oscillation device ontology, and the pivot structure includes the first support shaft and the second support shaft, and the first support shaft is set It sets in one end that lever is connect with claw-type structure, the second support shaft is arranged in the other end of lever, and the one of first support shaft The middle part of claw-type structure is run through at end, and the other end of the first support shaft is connected on oscillation device ontology, the middle part of the second support shaft Through lever, the both ends of the second support shaft are connect with oscillation device ontology.

10. dynamic cell culture device according to claim 9, which is characterized in that the oscillation device further includes two Gag lever post, two gag lever posts are separately positioned on the two sides of the lever of claw-type structure lower part, and one end of the gag lever post and oscillation fill It sets ontology to be fixedly connected, the other end of gag lever post is hanging.

11. dynamic cell culture device according to claim 10, which is characterized in that the first driving device is motor The side of device, the electric machine connects the first drive shaft；

Second driving device is electric machine, and the electric machine setting is driven in oscillation device body interior, described second Moving axis one end is connect with electric machine, and the other end of the second drive shaft is fixedly connected with second gear center.

12. dynamic cell culture device according to claim 10, which is characterized in that the first driving device is motor The side of device, the electric machine connects the first drive shaft；

Second driving device is clockwork, and the clockwork is arranged in oscillation device body interior, clockwork Side is arranged the second clockwork spring handle, and the second clockwork spring handle runs through after this body sidewall of oscillation device external, the hair that is exposed to oscillation device sheet The other side of strip device is fixedly connected by the second drive shaft with second gear center.

13. dynamic cell culture device according to claim 10, which is characterized in that the first driving device is clockwork spring The side of device, the clockwork connects the first drive shaft, and the other side of clockwork is provided with the first clockwork spring handle；

Second driving device is electric machine, and the electric machine setting is driven in oscillation device body interior, described second Moving axis one end is connect with electric machine, and the other end of the second drive shaft is fixedly connected with second gear center.

14. dynamic cell culture device according to claim 10, which is characterized in that the first driving device is clockwork spring The side of device, the clockwork connects the first drive shaft, and the other side of clockwork is provided with the first clockwork spring handle；

Second driving device is clockwork, and the clockwork is arranged in oscillation device body interior, clockwork Side is arranged the second clockwork spring handle, and the second clockwork spring handle runs through after this body sidewall of oscillation device external, the hair that is exposed to oscillation device sheet The other side of strip device is fixedly connected by the second drive shaft with second gear center.