CN112457138A - Microbial preparation for improving soil fertility of saline-alkali soil and preparation method thereof - Google Patents

Microbial preparation for improving soil fertility of saline-alkali soil and preparation method thereof Download PDF

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CN112457138A
CN112457138A CN202011520623.3A CN202011520623A CN112457138A CN 112457138 A CN112457138 A CN 112457138A CN 202011520623 A CN202011520623 A CN 202011520623A CN 112457138 A CN112457138 A CN 112457138A
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bran
microbial preparation
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saline
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李凤兰
张世红
何流琴
冯哲
***
冯艳忠
吴建
涂强
姜雪峰
胡雪峰
梁飞
冯旭
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Northeast Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C9/00Fertilisers containing urea or urea compounds
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/08Organic fertilisers containing added bacterial cultures, mycelia or the like
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/14Soil-conditioning materials or soil-stabilising materials containing organic compounds only

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  • Life Sciences & Earth Sciences (AREA)
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  • Soil Sciences (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Materials Engineering (AREA)
  • Pest Control & Pesticides (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses a microbial preparation for improving soil fertility of saline-alkali soil and a preparation method thereof. The preparation process of the microbial preparation comprises the following steps: step 1, adding single microbial agents forming the compound microorganism together according to a preset proportion, and uniformly mixing to obtain the compound microorganism; step 2, adding the raw materials together according to a set weight ratio, uniformly mixing, adding clear water, stirring and mixing to adjust the moisture content of the materials; and 3, fermenting the material obtained in the step 2 at the temperature of 18-28 ℃ to obtain the microbial preparation. The microbial preparation provided by the invention is applied to saline-alkali soil, can promote the growth of crops planted in the saline-alkali soil, reduce the salt content and pH value of the saline-alkali soil, and improve the physical and chemical properties of the soil.

Description

Microbial preparation for improving soil fertility of saline-alkali soil and preparation method thereof
Technical Field
The invention belongs to the technical field of saline-alkali soil improvement, and particularly relates to a microbial preparation for improving soil fertility of saline-alkali soil and a preparation method thereof.
Background
Saline-alkali soil is a general term for various salinized soil and alkaline soil. The salinization of the soil causes the farmland to be incapable of cultivating, the economic benefit of the soil is low, and the land resource is wasted. However, saline-alkali soil can be converted into cultivated land through improvement, which has important significance for sustainable development of agriculture.
The biological improvement of saline-alkali soil does not destroy the ecological environment, and the physical and chemical properties of soil and the microclimate of soil are improved mainly by introducing, screening and planting salt-tolerant plants.
Microorganisms in the soil can produce abundant metabolites during the own metabolic process. These metabolites may promote crop growth and increase the amount of minerals in the soil that may be assimilated by crops.
Therefore, the microbial preparation containing beneficial microorganisms is developed and applied to crops planted in saline-alkali soil, and the method has great significance for improving the saline-alkali soil.
Disclosure of Invention
The invention aims to solve the technical problem of providing a microbial preparation for improving the soil fertility of saline-alkali soil and a preparation method thereof.
On the one hand, the invention provides a microbial preparation for improving soil fertility of saline-alkali soil, which is mainly prepared from raw materials consisting of compound microorganisms, white sugar, urea, glutamine, oil bran, corn flour, wheat bran, rice bran and zeolite powder.
Preferably, the raw materials comprise, by weight, 5-10 parts of the compound microorganism, 5-10 parts of white sugar, 10-20 parts of urea, 0.5-10 parts of glutamine, 10-20 parts of oil bran, 10-20 parts of corn flour, 10-20 parts of wheat bran, 10-20 parts of rice bran and 2-5 parts of zeolite powder.
Preferably, the raw materials comprise, by weight, 5 parts of the compound microorganism, 8 parts of white sugar, 10 parts of urea, 0.5 part of glutamine, 10 parts of oil bran, 14 parts of corn flour, 20 parts of wheat bran, 10 parts of rice bran and 4 parts of zeolite powder.
Preferably, the raw materials comprise, by weight, 7 parts of the compound microorganism, 10 parts of white sugar, 15 parts of urea, 10 parts of glutamine, 15 parts of oil bran, 20 parts of corn flour, 16 parts of wheat bran, 15 parts of rice bran and 2 parts of zeolite powder.
Preferably, the raw materials comprise, by weight, 10 parts of the compound microorganism, 5 parts of white sugar, 20 parts of urea, 6 parts of glutamine, 20 parts of oil bran, 10 parts of corn flour, 10 parts of wheat bran, 20 parts of rice bran and 5 parts of zeolite powder.
Preferably, the complex microorganism comprises cercospora xanthioides, polyporus pinicola, trichoderma koningii, lactobacillus rhamnosus, candida utilis and bacillus licheniformis.
Preferably, the compound microorganism comprises 5-15 parts of the verticillium flavum, 2-8 parts of the polyporus piniper, 10-20 parts of the trichoderma koningii, 15-25 parts of the lactobacillus rhamnosus, 10-20 parts of the candida utilis and 5-10 parts of the bacillus licheniformis by weight.
In another aspect, the present invention also provides a method for preparing the microbial preparation, which comprises the following steps:
step 1, adding single microbial agents forming the compound microorganism together according to a preset proportion, and uniformly mixing to obtain the compound microorganism;
step 2, adding the raw materials together according to a set weight ratio, uniformly mixing, adding clear water, stirring and mixing to adjust the moisture content of the materials;
and 3, fermenting the material obtained in the step 2 at the temperature of 18-28 ℃ to obtain the microbial preparation.
Preferably, the moisture content of the material is 40-65%.
Preferably, the fermentation time of the material is 3-15 days.
The microbial preparation provided by the invention is applied to saline-alkali soil, can promote the growth of crops planted in the saline-alkali soil, reduce the salt content and pH value of the saline-alkali soil, improve the soil fertility, and improve the soil physicochemical property and ecological environment of the saline-alkali soil.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
The invention takes compound microorganism, white sugar, urea, glutamine, oil bran, corn flour, wheat bran, rice bran and zeolite powder as main raw materials, and the microbial preparation for improving the soil fertility of saline-alkali soil is obtained by fermentation. The microbial preparation provided by the invention is applied to saline-alkali soil, can promote the growth of crops planted in the saline-alkali soil, reduce the salt content and pH value of the saline-alkali soil, improve the soil fertility, and improve the soil physicochemical property and ecological environment of the saline-alkali soil.
In one embodiment of the invention, the microbial preparation for improving the soil fertility of the saline-alkali soil is prepared from raw materials consisting of compound microorganisms, white sugar, urea, glutamine, oil bran, corn flour, wheat bran, rice bran, zeolite powder and clear water. The specific preparation process comprises the following steps: step 1, preparing a compound microorganism; step 2, preparing a mixed material; and 3, fermenting the mixed material.
In the step 1 of preparing the composite microorganism, selecting powder of Talaromyces flavus, powder of Trichosporon roseus, powder of Trichoderma koningii, powder of Lactobacillus rhamnosus, powder of Candida utilis and powder of Bacillus licheniformis, and then compounding the six bacteria according to the proportioning relationship by weight to obtain the composite microorganism, wherein the composite microorganism is preferably 5-15 parts of powder of Talaromyces flavus, 2-8 parts of Pink polyporus pinus, 10-20 parts of Trichoderma koningii, 15-25 parts of Lactobacillus rhamnosus, 10-20 parts of Candida utilis and 5-10 parts of Bacillus licheniformis. On one hand, lactobacillus rhamnosus, candida utilis and bacillus licheniformis are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then the bacteria are separated from the fermentation culture and dried and concentrated to prepare single-strain solid bacterial powder. The bacterial content of each single bacterial strain powder is respectively as follows: the content of Lactobacillus rhamnosus is not less than 2.5 × 1011cfu/g, the bacteria content of Candida utilis is not less than 5.0 multiplied by 109cfu/g, the bacteria content of the bacillus licheniformis is more than or equal to 2.5 multiplied by 1010cfu/g. On the other hand, the bluish yellow fungus, the polyporus piniper and the trichoderma koningii are fermented in corresponding solid culture mediums respectively to obtain solid cultures, and then the solid cultures are dried and crushed to prepare single-strain solid fungus powder. The bacterial content of each single bacterial strain powder is respectively as follows: the content of the yellow blue fungus is not less than 2.5 × 1010cfu/g, the bacterium content of the polyporus pinicola is more than or equal to 3.5 multiplied by 1010cfu/g, the bacteria content of Trichoderma koningii is more than or equal to 3.0 multiplied by 1010cfu/g。
In the step 2 of preparing the mixed material, the compound microorganism, the white sugar, the urea, the glutamine, the oil bran, the corn meal, the wheat bran, the rice bran and the zeolite powder are weighed according to the preset weight proportion, then the nine raw materials are mixed and stirred evenly, and clear water is added to adjust the water content of the mixed material to be 40-65%. The weight proportions of the nine raw materials are respectively optimized as follows: 5-10 parts of compound microorganism, 5-10 parts of white sugar, 10-20 parts of urea, 0.5-10 parts of glutamine, 10-20 parts of oil bran, 10-20 parts of corn flour, 10-20 parts of wheat bran, 10-20 parts of rice bran and 2-5 parts of zeolite powder.
In the step 3 of fermenting the mixed material, the initial pH of the mixed material is natural, and then the mixed material is subjected to facultative fermentation at the temperature of 18-28 ℃ for 3-15 days. The fermented product is used as a microbial preparation for improving the soil fertility of saline-alkali soil.
To help better understand the technical solution of the present invention, the following examples are provided to illustrate the preparation process of the microbial preparation for improving soil fertility of saline-alkali soil according to the present invention.
Example one
The raw materials of the microbial preparation for improving the soil fertility of the saline-alkali soil mainly comprise 5 parts of compound microorganisms, 8 parts of white sugar, 10 parts of urea, 0.5 part of glutamine, 10 parts of oil bran, 14 parts of corn flour, 20 parts of wheat bran, 10 parts of rice bran and 4 parts of zeolite powder by weight. The composite microorganism comprises, by weight, 5 parts of a fulvia fulvescens powder, 5 parts of a pink polyporus gramineus powder, 20 parts of a trichoderma koningii powder, 19 parts of a rhamnose lactobacillus powder, 16 parts of a candida utilis powder and 10 parts of a bacillus licheniformis powder. The six bacterial powders can be prepared by self, on one hand, lactobacillus plantarum, candida utilis and bacillus megatherium are respectively fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then the bacterial bodies are separated from the fermented culture and driedDrying and concentrating to obtain single-strain solid powder. The bacterial contents of the three single bacterial strains are respectively as follows: the content of Lactobacillus rhamnosus is not less than 2.5 × 1011cfu/g, the bacteria content of Candida utilis is not less than 5.0 multiplied by 109cfu/g, the bacteria content of the bacillus licheniformis is more than or equal to 2.5 multiplied by 1010cfu/g. On the other hand, the bluish yellow fungus, the polyporus piniper and the trichoderma koningii are fermented in corresponding solid culture mediums respectively to obtain solid cultures, and then the solid cultures are dried and crushed to prepare single-strain solid fungus powder. The bacterial contents of the three single bacterial strains are respectively as follows: the content of the yellow blue fungus is not less than 2.5 × 1010cfu/g, the bacterium content of the polyporus pinus is more than or equal to 3.5 multiplied by 1010cfu/g, the bacteria content of Trichoderma koningii is more than or equal to 3.0 multiplied by 1010cfu/g。
The microbial preparation for improving the soil fertility of the saline-alkali soil is prepared by the following steps.
Step 1, preparing a composite microorganism, and uniformly mixing powder of a ceruleus flavedo fungus, powder of a polyporus pinus fungus, powder of trichoderma koningii, powder of lactobacillus rhamnosus, powder of candida utilis and powder of bacillus licheniformis to obtain the composite microorganism.
And 2, mixing the raw materials to prepare a mixed material, adding the compound microorganism, white sugar, urea, glutamine, oil bran, corn flour, wheat bran, rice bran and zeolite powder together, mixing and stirring uniformly, and adding clear water into the uniformly mixed material to adjust the water content of the mixed material to 40%.
And 3, fermenting, wherein the initial pH of the material is natural, and then carrying out facultative fermentation for 3 days at the temperature of 18-28 ℃. The fermented product is used as a microbial preparation 1 for improving the soil fertility of saline-alkali soil.
Example two
The raw materials of the microbial preparation for improving the soil fertility of the saline-alkali soil mainly comprise 7 parts by weight of compound microorganisms, 10 parts by weight of white sugar, 15 parts by weight of urea, 10 parts by weight of glutamine, 15 parts by weight of oil bran, 20 parts by weight of corn flour, 16 parts by weight of wheat bran, 15 parts by weight of rice bran and 2 parts by weight of zeolite powder. Wherein the compound microorganism is selected from the group consisting of Talaromyces flavipes powder, Pleuromyces roseus powder, Trichoderma koningii powder, and Lactobacillus rhamnosusThe bacillus licheniformis powder comprises 11 parts of verticillium flavum powder, 2 parts of pinkish polyporus pinus powder, 14 parts of trichoderma koningii powder, 15 parts of lactobacillus rhamnosus powder, 20 parts of candida utilis powder and 5 parts of bacillus licheniformis powder. On one hand, lactobacillus plantarum, candida utilis and bacillus megatherium are fermented and cultured in corresponding liquid culture media respectively to obtain bacterial liquids, and then the bacteria are separated from the fermentation culture and dried and concentrated to prepare single-strain solid bacterial powder. The bacterial contents of the three single bacterial strains are respectively as follows: the content of Lactobacillus rhamnosus is not less than 2.5 × 1011cfu/g, the bacteria content of Candida utilis is not less than 5.0 multiplied by 109cfu/g, the bacteria content of the bacillus licheniformis is more than or equal to 2.5 multiplied by 1010cfu/g. On the other hand, the bluish yellow fungus, the polyporus piniper and the trichoderma koningii are fermented in corresponding solid culture mediums respectively to obtain solid cultures, and then the solid cultures are dried and crushed to prepare single-strain solid fungus powder. The bacterial contents of the three single bacterial strains are respectively as follows: the content of the yellow blue fungus is not less than 2.5 × 1010cfu/g, the bacterium content of the polyporus pinus is more than or equal to 3.5 multiplied by 1010cfu/g, the bacteria content of Trichoderma koningii is more than or equal to 3.0 multiplied by 1010cfu/g。
The microbial preparation for improving the soil fertility of the saline-alkali soil is prepared by the following steps.
Step 1, preparing a composite microorganism, and uniformly mixing powder of a ceruleus flavedo fungus, powder of a polyporus pinus fungus, powder of trichoderma koningii, powder of lactobacillus rhamnosus, powder of candida utilis and powder of bacillus licheniformis to obtain the composite microorganism.
And 2, mixing the raw materials to prepare a mixed material, adding the compound microorganism, white sugar, urea, glutamine, oil bran, corn flour, wheat bran, rice bran and zeolite powder together, mixing and stirring uniformly, and adding clear water into the uniformly mixed material to adjust the water content of the mixed material to be 50%.
And 3, fermenting, wherein the initial pH of the material is natural, and then carrying out facultative fermentation for 9 days at the temperature of 18-28 ℃. The fermented product is used as a microbial preparation 2 for improving the soil fertility of the saline-alkali soil.
EXAMPLE III
The raw materials of the microbial preparation for improving the soil fertility of the saline-alkali soil mainly comprise, by weight, 10 parts of compound microorganisms, 5 parts of white sugar, 20 parts of urea, 6 parts of glutamine, 20 parts of oil bran, 10 parts of corn flour, 10 parts of wheat bran, 20 parts of rice bran and 5 parts of zeolite powder. The composite microorganism comprises 15 parts of albonococcum powder, 8 parts of pinkish polyporus pinicolus powder, 10 parts of trichoderma koningii powder, 25 parts of rhamnose lactobacillus powder, 10 parts of candida utilis powder and 7 parts of bacillus licheniformis powder. On one hand, lactobacillus plantarum, candida utilis and bacillus megatherium are fermented and cultured in corresponding liquid culture media respectively to obtain bacterial liquids, and then the bacteria are separated from the fermentation culture and dried and concentrated to prepare single-strain solid bacterial powder. The bacterial contents of the three single bacterial strains are respectively as follows: the content of Lactobacillus rhamnosus is not less than 2.5 × 1011cfu/g, the bacteria content of Candida utilis is not less than 5.0 multiplied by 109cfu/g, the bacteria content of the bacillus licheniformis is more than or equal to 2.5 multiplied by 1010cfu/g. On the other hand, the bluish yellow fungus, the polyporus piniper and the trichoderma koningii are fermented in corresponding solid culture mediums respectively to obtain solid cultures, and then the solid cultures are dried and crushed to prepare single-strain solid fungus powder. The bacterial contents of the three single bacterial strains are respectively as follows: the content of the yellow blue fungus is not less than 2.5 × 1010cfu/g, the bacterium content of the polyporus pinus is more than or equal to 3.5 multiplied by 1010cfu/g, the bacteria content of Trichoderma koningii is more than or equal to 3.0 multiplied by 1010cfu/g。
The microbial preparation for improving the soil fertility of the saline-alkali soil is prepared by the following steps.
Step 1, preparing a composite microorganism, and uniformly mixing powder of a ceruleus flavedo fungus, powder of a polyporus pinus fungus, powder of trichoderma koningii, powder of lactobacillus rhamnosus, powder of candida utilis and powder of bacillus licheniformis to obtain the composite microorganism.
And 2, mixing the raw materials to prepare a mixed material, adding the compound microorganism, white sugar, urea, glutamine, oil bran, corn flour, wheat bran, rice bran and zeolite powder together, mixing and stirring uniformly, and adding clear water into the uniformly mixed material to adjust the water content of the mixed material to 65%.
And 3, fermenting, wherein the initial pH of the material is natural, and then carrying out facultative fermentation for 15 days at the temperature of 18-28 ℃. The fermented product is used as a microbial preparation 3 for improving the soil fertility of the saline-alkali soil.
To help better understand the technical scheme of the present invention, a test example of rice planting is provided below to illustrate the application effect of the microbial preparation of the present invention.
Test example: application effect of microbial preparation on saline-alkali soil improvement and influence on rice growth
A saline-alkali field block is selected in Ningxia Yinchuan city, the basic physicochemical properties of the field plough layer soil are that the pH value is 8.38, the total salt content is 1.86g/kg, the organic matter content is 0.97%, the total nitrogen content is 1.17g/kg, the total phosphorus content is 0.58g/kg, the quick-acting phosphorus content is 34.5mg/kg, the quick-acting potassium content is 318mg/kg, and the alkaline hydrolysis nitrogen content is 41.8 mg/kg. 4 groups of experimental designs, including 3 experimental groups and 1 control group, each group of experimental designs 3 experimental cells, each area of the experimental cells is 30m2All test cells are randomly distributed.
The test groups applied fertilizers and microbial preparations prepared according to the invention. The application method is that urea, calcium superphosphate and microbial preparation are applied to soil as base fertilizer before rice is planted, the three fertilizers are uniformly scattered on the surface of the soil, and then ploughing is carried out. Wherein the fertilizing amount of the two fertilizers is 700kg/hm of urea respectively2680kg/hm of calcium superphosphate2. Microbial preparation 1-microbial preparation 3 were applied to 3 test groups, respectively, and the application rates of the three microbial preparations were 30kg/hm2Microbial preparation 1, 45kg/hm2Microbial preparation 2, 60kg/hm2And (3) a microbial preparation. The control group was not administered with the microbial preparation, and the rest of the procedure was the same as the test group.
Selecting Ningjing 41 as a test variety, applying fertilizer according to a predetermined scheme in the last 5 months, and preparing soil. After 10 days, the seedlings are transplanted, the row spacing is 20cm, the plant spacing is 10cm, and 5 seedlings are transplanted in each hole. The same conventional management is adopted in each plot, and 10 rice plants are randomly selected from each plot in the tillering stage of the rice to determine the tillering number, the plant height, the root length and the leaf area. And harvesting the rice in the last ten days of 9 months, and counting the rice yield of each cell. The average tillering number, average plant height, average root length, average leaf area and average cell rice yield of each group were calculated. The results are shown in Table 1.
TABLE 1
Figure BDA0002848792320000071
As can be seen from the data in Table 1, the 3 groups of rice seeds applied with the microbial preparation were significantly higher than the control in terms of tiller number, plant height, root length, leaf area and 5 shapes of rice yield in the small area. Therefore, the prepared microbial preparations 1-3 can promote the growth of rice and improve the yield of rice.
In addition, after the rice is harvested, soil samples are taken from each cell, the pH, the total salt content, the organic matter content, the total nitrogen content, the total phosphorus content, the alkaline hydrolysis nitrogen content, the quick-acting phosphorus content and the quick-acting potassium content of the soil are measured, and the average pH, the average total salt content, the average organic matter content, the average total nitrogen content, the average total phosphorus content, the average alkaline hydrolysis nitrogen content, the average quick-acting phosphorus content and the average quick-acting potassium content of each group of soil are calculated. The results are shown in Table 2.
TABLE 2
Figure BDA0002848792320000072
Figure BDA0002848792320000081
As can be seen from the data in Table 2, the pH and the total salt content of the rice planting soil of 3 groups applied with the microbial preparation are both obviously lower than those of the control, and the organic matter content, the total nitrogen content, the total phosphorus content, the alkaline hydrolysis nitrogen content, the quick-acting phosphorus content and the quick-acting potassium content are all obviously higher than those of the control. Therefore, after the prepared microbial agents 1-3 are applied to saline-alkali soil, the pH value and the salt content of the soil can be reduced, the physical and chemical properties of the soil can be improved, and the soil fertility can be improved.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.

Claims (10)

1. A microbial preparation for improving soil fertility of saline-alkali soil is mainly prepared from composite microorganisms, white sugar, urea, glutamine, oil bran, corn flour, wheat bran, rice bran and zeolite powder.
2. The microbial formulation of claim 1, wherein: the raw materials comprise, by weight, 5-10 parts of the compound microorganism, 5-10 parts of white sugar, 10-20 parts of urea, 0.5-10 parts of glutamine, 10-20 parts of oil bran, 10-20 parts of corn flour, 10-20 parts of wheat bran, 10-20 parts of rice bran and 2-5 parts of zeolite powder.
3. The microbial preparation of claim 1 or 2, wherein: the raw materials comprise, by weight, 5 parts of the compound microorganism, 8 parts of white sugar, 10 parts of urea, 0.5 part of glutamine, 10 parts of oil bran, 14 parts of corn flour, 20 parts of wheat bran, 10 parts of rice bran and 4 parts of zeolite powder.
4. The microbial preparation of claim 1 or 2, wherein: the raw materials comprise, by weight, 7 parts of the compound microorganism, 10 parts of white sugar, 15 parts of urea, 10 parts of glutamine, 15 parts of oil bran, 20 parts of corn flour, 16 parts of wheat bran, 15 parts of rice bran and 2 parts of zeolite powder.
5. The microbial preparation of claim 1 or 2, wherein: the raw materials comprise, by weight, 10 parts of the compound microorganism, 5 parts of white sugar, 20 parts of urea, 6 parts of glutamine, 20 parts of oil bran, 10 parts of corn flour, 10 parts of wheat bran, 20 parts of rice bran and 5 parts of zeolite powder.
6. The microbial preparation of claim 1 or 2, wherein: the compound microorganism comprises albonophyte, stenotrophospora rosea, trichoderma koningii, lactobacillus rhamnosus, candida utilis and bacillus licheniformis.
7. The microbial formulation of claim 6, wherein: the compound microorganism comprises, by weight, 5-15 parts of albonophyte, 2-8 parts of telangium roseum, 10-20 parts of trichoderma koningii, 15-25 parts of lactobacillus rhamnosus, 10-20 parts of candida utilis and 5-10 parts of bacillus licheniformis.
8. A process for the preparation of a microbial preparation according to any one of claims 1 to 7, comprising the steps of:
step 1, adding single microbial agents forming the compound microorganism together according to a preset proportion, and uniformly mixing to obtain the compound microorganism;
step 2, adding the raw materials together according to a set weight ratio, uniformly mixing, adding clear water, stirring and mixing to adjust the moisture content of the materials;
and 3, fermenting the material obtained in the step 2 at the temperature of 18-28 ℃ to obtain the microbial preparation.
9. The method of claim 8, wherein: the moisture content of the material is 40-65%.
10. The method of claim 8, wherein: the fermentation time of the material is 3-15 days.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112586511A (en) * 2020-11-21 2021-04-02 东北农业大学 Seed dressing agent for promoting germination of crop seeds planted in saline-alkali soil and seed treatment method

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