CN103483073A - Special compound microorganism bacterial fertilizer for peanuts and production method thereof - Google Patents
Special compound microorganism bacterial fertilizer for peanuts and production method thereof Download PDFInfo
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- 235000020232 peanut Nutrition 0.000 title claims abstract description 63
- 239000003337 fertilizer Substances 0.000 title claims abstract description 51
- 244000005700 microbiome Species 0.000 title claims abstract description 42
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 26
- 150000001875 compounds Chemical class 0.000 title abstract description 6
- 238000004519 manufacturing process Methods 0.000 title abstract description 5
- 241001553178 Arachis glabrata Species 0.000 title abstract 7
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 43
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical group O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 claims abstract description 27
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 14
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 14
- 241000894006 Bacteria Species 0.000 claims description 135
- 239000000843 powder Substances 0.000 claims description 71
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 56
- 244000105624 Arachis hypogaea Species 0.000 claims description 56
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 56
- 235000018262 Arachis monticola Nutrition 0.000 claims description 56
- 239000007788 liquid Substances 0.000 claims description 52
- 238000000855 fermentation Methods 0.000 claims description 46
- 230000004151 fermentation Effects 0.000 claims description 46
- 238000002360 preparation method Methods 0.000 claims description 42
- 241000193417 Brevibacillus laterosporus Species 0.000 claims description 34
- 238000003756 stirring Methods 0.000 claims description 30
- 241000194108 Bacillus licheniformis Species 0.000 claims description 29
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 29
- 239000011591 potassium Substances 0.000 claims description 29
- 229910052700 potassium Inorganic materials 0.000 claims description 29
- 229910019142 PO4 Inorganic materials 0.000 claims description 28
- 235000021317 phosphate Nutrition 0.000 claims description 28
- 239000002068 microbial inoculum Substances 0.000 claims description 25
- 238000004659 sterilization and disinfection Methods 0.000 claims description 22
- 241000223259 Trichoderma Species 0.000 claims description 17
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 17
- 239000004202 carbamide Substances 0.000 claims description 17
- 238000009423 ventilation Methods 0.000 claims description 17
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 16
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 16
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 15
- 229920002472 Starch Polymers 0.000 claims description 15
- 238000007605 air drying Methods 0.000 claims description 15
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 15
- 239000001110 calcium chloride Substances 0.000 claims description 15
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 15
- 229940041514 candida albicans extract Drugs 0.000 claims description 15
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 15
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 15
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 15
- 235000019800 disodium phosphate Nutrition 0.000 claims description 15
- 238000005189 flocculation Methods 0.000 claims description 15
- 230000016615 flocculation Effects 0.000 claims description 15
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 15
- 239000001301 oxygen Substances 0.000 claims description 15
- 229910052760 oxygen Inorganic materials 0.000 claims description 15
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 15
- 239000010452 phosphate Substances 0.000 claims description 15
- 238000000926 separation method Methods 0.000 claims description 15
- 235000019698 starch Nutrition 0.000 claims description 15
- 239000008107 starch Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000012138 yeast extract Substances 0.000 claims description 15
- 239000004033 plastic Substances 0.000 claims description 14
- 229920003023 plastic Polymers 0.000 claims description 14
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 239000004254 Ammonium phosphate Substances 0.000 claims description 13
- 208000006558 Dental Calculus Diseases 0.000 claims description 13
- ZRIUUUJAJJNDSS-UHFFFAOYSA-N ammonium phosphates Chemical group [NH4+].[NH4+].[NH4+].[O-]P([O-])([O-])=O ZRIUUUJAJJNDSS-UHFFFAOYSA-N 0.000 claims description 13
- 235000019289 ammonium phosphates Nutrition 0.000 claims description 13
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims description 13
- 230000002051 biphasic effect Effects 0.000 claims description 10
- 238000012364 cultivation method Methods 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- 238000010298 pulverizing process Methods 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 9
- 241000223260 Trichoderma harzianum Species 0.000 claims description 8
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 7
- 240000007594 Oryza sativa Species 0.000 claims description 7
- 235000007164 Oryza sativa Nutrition 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 238000006385 ozonation reaction Methods 0.000 claims description 7
- 235000009566 rice Nutrition 0.000 claims description 7
- 229910001220 stainless steel Inorganic materials 0.000 claims description 7
- 239000010935 stainless steel Substances 0.000 claims description 7
- 235000015099 wheat brans Nutrition 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 5
- 239000002131 composite material Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 230000000855 fungicidal effect Effects 0.000 claims description 4
- 239000000417 fungicide Substances 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 2
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- 230000008859 change Effects 0.000 abstract description 2
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 abstract 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 abstract 1
- 235000019838 diammonium phosphate Nutrition 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 abstract 1
- 229910052939 potassium sulfate Inorganic materials 0.000 abstract 1
- 239000001120 potassium sulphate Substances 0.000 abstract 1
- 235000011151 potassium sulphates Nutrition 0.000 abstract 1
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
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- 235000002918 Fraxinus excelsior Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
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Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention belongs to the technical field of fertilizer, particularly relates to special full-dissolubility compound microorganism bacterial fertilizer for peanuts and further relates to a production method of the special fertilizer for the peanuts. The special compound microorganism bacterial fertilizer for the peanuts comprises, by weight, 1-5 parts of compound microorganism bacterial agent, 5-10 parts of furfural residue, 5-25 parts of ammonium sulfate, 5-20 parts of monopotassium phosphate, 5-20 parts of diammonium hydrogen phosphate and 5-20 parts of potassium sulphate. The fertilizer can effectively prevent root rot of the peanuts, can change the micro-ecological environment of soil on the peripheries of root systems of the peanuts, can increase breathability of the soil, eliminate soil hardening and neutralize alkalinity, and can make yield of the peanuts increased by at least 20%.
Description
Technical field
The invention belongs to technical field of fertilizers, be specifically related to a kind of full dissolubility peanut specific complex microbial-bacterial fertilizer, also relate to the production method of this fertilizer specially for peanut.
background technology
Peanut is a kind of important oil crops, plant in massif gravel soil, Plain atteration and southern red yellow earth China is multiple, these soil ubiquities the problem of dysplasia, nutritive ingredient shortage, therefore, the special fertilizer of peanut cultivation should be with activating soil, the improvement soil microenvironment, the organic fertilizer that increases fertility is main, chemical fertilizer is auxiliary.At present, during peanut cultivation, ubiquity fertilising kind, the extremely irrational phenomenon of nutrition configuration, ignores the reasonably combined of nutritive element, causes the soil nutrient serious unbalance, affects the yield and quality of peanut.In addition in recent years, because the peanut continuous cropping time limit extends, the problems such as peanut disease bacterium resistance, peanut disease is day by day serious, especially the most serious with the Roots of Peanut maize ear rot, common prevention and treatment method is the medication again of when morbidity, but the yield and quality of peanut has been caused to impact.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides and a kind ofly can effectively prevent and treat the generation of Roots of Peanut maize ear rot, change Peanut Root System surrounding soil micro-ecological environment, increase ventilation property, the elimination soil compaction of soil, neutralization alkalescence, can make the peanut specific complex microbial-bacterial fertilizer of peanut yield increasing at least 20%, the present invention is by Bacillus licheniformis, bacillus laterosporus and trichoderma harziarum, and acting in conjunction is in soil;
The present invention also provides above-mentioned its production method of bacterial manure, the method is after spawn culture, to be administered in soil, flora microbial decomposition in fertilizer, constantly discharging various nutrients absorbs for peanut, and microorganism species is in the micro-ecological environment around Peanut Root System, occupy growth vigor, but the generation of effectively preventing Roots of Peanut maize ear rot.
The one specific complex microbial-bacterial fertilizer of cultivating peanut, this fertilizer comprises the component of following portions by weight:
1～5 part of complex microorganism bacteria agent
5～10 parts of furfural dregs
5～25 parts, ammonium sulfate
5～20 parts of Secondary ammonium phosphates
5～20 parts of vitriolate of tartar
5～20 parts of potassium primary phosphates.
Preferably, above-mentioned fertilizer comprises the component of following parts by weight:
2～4 parts of complex microorganism bacteria agents
6～8 parts of furfural dregs
10～20 parts, ammonium sulfate
10～16 parts of Secondary ammonium phosphates
10～16 parts of vitriolate of tartar
10～16 parts of potassium primary phosphates.
Preferred, above-mentioned fertilizer comprises the component of following parts by weight:
3 parts of complex microorganism bacteria agents
8 parts of furfural dregs
16 parts, ammonium sulfate
15 parts of Secondary ammonium phosphates
15 parts of vitriolate of tartar
15 parts of potassium primary phosphates.
The preparation process of complex microorganism bacteria agent of the present invention is as follows: by Bacillus licheniformis, bacillus laterosporus and three independent inoculation fermentations of bacterial classifications difference of trichoderma harziarum, the lichen bacillus ferments liquid, bacillus laterosporus fermented liquid obtain Bacillus licheniformis powder, bacillus laterosporus powder through drying to pulverize respectively; Trichoderma harziarum zymophyte piece obtains the Trichoderma powder through drying in the shade, pulverizing, finally three kinds of bacterium powder are mixed, and obtains the complex microorganism bacteria agent.
Above-mentioned composite bacillus microbial inoculum preparation process is specially:
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, Bacillus licheniformis strain is inoculated in disinfection fermentation tank, fermention medium is: starch 0.2-0.8%, yeast extract paste 0.01-0.06%, dregs of beans 0.2-0.8%, urea 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, sal epsom 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 35-40 ℃, stir revolution 100-200rpm, dissolved oxygen is not less than 30%, the pH value is not less than 6.5, stir culture 6-8 hour, make viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, 45-50 ℃ of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, put into pulverizer and pulverize, and crosses 30 mesh sieves and obtain the bacterium powder,
Bacillus laterosporus microbial inoculum preparation: the bacillus laterosporus bacterial classification is inoculated in disinfection fermentation tank, fermention medium is: starch 0.2-0.8%, yeast extract paste 0.01-0.06%, dregs of beans 0.2-0.8%, urea 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, sal epsom 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 35-40 ℃, stir revolution 100-200rpm, dissolved oxygen is not less than 30%, the pH value is not less than 6.5, stir culture 6-8 hour, make viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, 45-50 ℃ of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, put into pulverizer and pulverize, and crosses 30 mesh sieves and obtain the bacterium powder,
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, conidium liquid is proceeded in the PD liquid nutrient medium, shaking culture 2-4 days, then the part by weight according to 1:6-10 evenly mixes with rice bran or the wheat bran of sterilizing, be placed in the stainless steel mesh screen through liming or ozonization, the above covers the plastics film of sterilization, after being placed in 22-30 ℃ indoor cultivation 3-5 days, open plastics film, produce spore 3-5 days; Together with culture material, collect, the moon 5-7 days that dries in the air, after putting into pulverizer and pulverizing, cross 30 mesh sieves and make the bacterium powder;
The preparation of complex microorganism bacteria agent:
The Bacillus licheniformis powder, bacillus laterosporus bacterium powder, the trichoderma harzianum bacterium powder that obtain in above-mentioned steps are mixed according to the ratio of 1-4:1-4:1, obtain the complex microorganism bacteria agent.
Get 5～10 parts of furfural dregs, 5～25 parts, ammonium sulfate, 5～20 parts of Secondary ammonium phosphates, 5～20 parts of vitriolate of tartar, 5～20 parts of potassium primary phosphates, above-mentioned raw material is mixed, under 90 ℃, dry, send into crusher in crushing powdered after, add 1～5 part of mixing of complex microorganism bacteria agent prepared by above-mentioned step, obtain peanut specific complex microbial-bacterial fertilizer.
As a modification of the present invention, in the preparation method of peanut specific complex microbial-bacterial fertilizer, composite bacillus microbial inoculum preparation process is specially:
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, and Bacillus licheniformis strain is inoculated in disinfection fermentation tank, and fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, sal epsom 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5,37 ℃ of culture temperature, stir revolution 150rpm, and dissolved oxygen is not less than 30%, the pH value is not less than 6.5, and stir culture 7 hours makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, bacterium mud is in 45 ℃ of lower forced air dryings to water content 30%, cyclonic separation bacterium powder, put into pulverizer and pulverized, and crosses 30 mesh sieves and obtain the bacterium powder;
Bacillus laterosporus microbial inoculum preparation: the bacillus laterosporus bacterial classification is inoculated in disinfection fermentation tank, and fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, sal epsom 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5,37 ℃ of culture temperature, stir revolution 150rpm, dissolved oxygen is not less than 30%, pH value and is not less than 6.5, stir culture 7 hours, make viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, bacterium mud is in 45 ℃ of lower forced air dryings to water content 30%, cyclonic separation bacterium powder, put into pulverizer and pulverized, and crosses 30 mesh sieves and obtain the bacterium powder;
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, cultural method is liquid rotating solid biphasic cultivation method, conidium liquid is proceeded in the PD liquid nutrient medium, shaking culture 3 days, then the part by weight according to 1:8 evenly mixes with rice bran or the wheat bran of sterilizing, is placed in the stainless steel mesh screen through liming or ozonization, the above covers the plastics film of sterilization, be placed under 28 ℃ and after 4 days, open plastics film at indoor cultivation, produce spore 4 days; Together with culture material, collect, the moon dries in the air 6 days, after putting into the pulverizer pulverizing, crosses 30 mesh sieves and makes the bacterium powder;
The complex micro organism fungicide preparation:
The Bacillus licheniformis powder, bacillus laterosporus bacterium powder, the trichoderma harzianum bacterium powder that obtain in above step are mixed according to the ratio of 2:2:1, obtain the complex microorganism bacteria agent.
In the present invention, the complex micro organism fungicide in base fertilizer adopts the bacterium powder of the lichen bacillus ferments liquid dry powder, bacillus laterosporus fermented liquid dry powder and these three kinds of bacterium of trichoderma harzianum powder to mix.
Beneficial effect after the present invention uses peanut is, fertilizer provided by the present invention can effectively be prevented and treated the generation of Roots of Peanut maize ear rot, changes Peanut Root System surrounding soil micro-ecological environment, increases ventilation property, the elimination soil compaction of soil, neutralization alkalescence, can make peanut yield increasing at least 20%.
The principle that produces above beneficial effect is: after being administered in soil by the special-purpose bacterial manure of peanut of the present invention, the microbial decomposition of flora in fertilizer, can discharge continuously various nutrients absorbs for peanut, simultaneously, the organic acid produced in the microorganism decomposition course and mineral acid, effectively the alkalescence of sweetening of the soil, be conducive to the growth of peanut.
In addition, microorganism species, in the micro-ecological environment around Peanut Root System, occupies growth vigor, can effectively prevent and treat the generation of Roots of Peanut maize ear rot.
The innovative point of above invention is:
The selection of bacterial classification: the compound bacterial fertilizer of Bacillus licheniformis, bacillus laterosporus and trichoderma harziarum, with existing compound bacterial fertilizer, compare, through field test, obviously promote the offspring growth, effectively resist the peanut disease and pest, aspect raising peanut yield and quality, there is obvious advantage.
Microbial-bacterial fertilizer bacterium viable bacteria content reaches 1,000 hundred million/grams, and considerably beyond domestic other products, reason is, three of the present invention the adopted strain bacterial classifications zymotechniques that premenstruum, research was established, can obtain stable high viable bacteria content; In the microbial inoculum preparation process, strictly control bake out temperature, at utmost guarantee high viable bacteria content, can improve 20 times of left and right of viable bacteria content.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
Embodiment 1
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, and Bacillus licheniformis strain is inoculated in disinfection fermentation tank, and fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, sal epsom 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5,37 ℃ of culture temperature, stir revolution 150rpm, and dissolved oxygen is not less than 30%, the pH value is not less than 6.5, and stir culture 7 hours makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, and 45-50 ℃ of forced air drying of bacterium mud is to water content 30%, and cyclonic separation bacterium powder, put into pulverizer and pulverized, and crosses 30 mesh sieves and obtain the bacterium powder;
Bacillus laterosporus microbial inoculum preparation: the bacillus laterosporus bacterial classification is inoculated in disinfection fermentation tank, and fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, sal epsom 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5,37 ℃ of culture temperature, stir revolution 150rpm, dissolved oxygen is not less than 30%, pH value and is not less than 6.5, stir culture 7 hours, make viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, and 45 ℃ of forced air dryings of bacterium mud are to water content 30%, and cyclonic separation bacterium powder, put into pulverizer and pulverized, and crosses 30 mesh sieves and obtain the bacterium powder;
Bacillus laterosporus microbial inoculum preparation: the bacillus laterosporus bacterial classification is inoculated in disinfection fermentation tank, and fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, sal epsom 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5,37 ℃ of culture temperature, stir revolution 150rpm, dissolved oxygen is not less than 30%, pH value and is not less than 6.5, stir culture 7 hours, make viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, bacterium mud is in 45 ℃ of lower forced air dryings to water content 30%, cyclonic separation bacterium powder, put into pulverizer and pulverized, and crosses 30 mesh sieves and obtain the bacterium powder;
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, cultural method is liquid rotating solid biphasic cultivation method, conidium liquid is proceeded in the PD liquid nutrient medium, shaking culture 3 days, then the part by weight according to 1:8 evenly mixes with rice bran or the wheat bran of sterilizing, is placed in the stainless steel mesh screen through liming or ozonization, the above covers the plastics film of sterilization, be placed under 28 ℃ and after 4 days, open plastics film at indoor cultivation, produce spore 4 days; Together with culture material, collect, the moon dries in the air 6 days, after putting into the pulverizer pulverizing, crosses 30 mesh sieves and makes the bacterium powder;
The complex micro organism fungicide preparation:
The Bacillus licheniformis powder, bacillus laterosporus bacterium powder, the trichoderma harzianum bacterium powder that obtain in above step are mixed according to the ratio of 2:2:1, obtain the complex microorganism bacteria agent.
Get 8 parts of furfural dregs, 16 parts, ammonium sulfate, 15 parts of Secondary ammonium phosphates, 15 parts of vitriolate of tartar, 15 parts of potassium primary phosphates, above-mentioned raw material is mixed, under 90 ℃, dry, send into crusher in crushing powdered after, add the 3 parts of mixing of complex microorganism bacteria agent that prepare by above-mentioned step, obtain peanut specific complex microbial-bacterial fertilizer.
Comparative Examples 1
With the difference of embodiment 1, be, in Comparative Examples 1, the complex microorganism bacteria agent only comprises the combination of bacillus licheniformis agent, these two kinds of microbial inoculums of bacillus laterosporus microbial inoculum, and the add-on of two kinds of bacterium is total up to 3 parts, and its weight ratio is 1:1; Preparation method and the embodiment 1 of two kinds of above-mentioned bacterial classifications are identical, and remaining step is also identical;
Comparative Examples 2
With the difference of embodiment 1, be, in Comparative Examples 1, the complex microorganism bacteria agent only comprises the combination of bacillus licheniformis agent, these two kinds of microbial inoculums of trichoderma harziarum bacteria agent, and the add-on of two kinds of bacterium is total up to 3 parts, and its weight ratio is 1:1; Preparation method and the embodiment 1 of two kinds of above-mentioned bacterial classifications are identical, and remaining step is also identical;
Comparative Examples 3
With the difference of embodiment 1, be, in Comparative Examples 1, the complex microorganism bacteria agent only comprises the combination of bacillus laterosporus microbial inoculum, these two kinds of microbial inoculums of trichoderma harziarum bacteria agent, and the add-on of two kinds of bacterium is total up to 3 parts, and its weight ratio is 1:1; Preparation method and the embodiment 1 of two kinds of above-mentioned bacterial classifications are identical, and remaining step is also identical;
In above embodiment 1 and Comparative Examples 1,2,3, can find out, the combination of three kinds of bacterial classifications of the present invention, its fertilizer efficiency is good, and data are as follows:
Choose same piece of land, be divided into 4, use respectively the fertilizer of embodiment 1, Comparative Examples 1,2,3, its fertilizer efficiency is compared as follows:
Embodiment 1, Comparative Examples 1,2,3 are used soil front and back fertility contrasts (mg/kg of unit) (available nitrogen)
Embodiment 1, Comparative Examples 1,2,3 are used soil front and back fertility contrasts (mg/kg of unit) (rapid available phosphorus)
Embodiment 1, Comparative Examples 1,2,3 are used soil front and back fertility contrasts (mg/kg of unit) (available potassium)
Field experiment
Choose the same soil, be divided into 4 parts, the peanut of same kind in sowing, when peanut starts to emerge, use respectively the fertilizer of identical weight in these 4 grounds, use embodiment 1, Comparative Examples 1, Comparative Examples 2, Comparative Examples 3 fertilizer after, after the growth regular hour, peanut seedling growth result is as follows:
Output texts after harvesting peanut
From above data, can find out relatively, after fertilizer of the present invention is appended in the soil of plantation peanut, its fertilizer efficiency is better than in Comparative Examples 1,2,3 any one group, visible, the combination of these three kinds of bacterial classifications of the present invention is better than the combined effect of any two kinds in above three kinds of bacterial classifications.
In embodiment 1, the sickness rate of Roots of Peanut maize ear rot is 2%;
In Comparative Examples 1, the sickness rate of Roots of Peanut maize ear rot is 11%;
In Comparative Examples 2, the sickness rate of Roots of Peanut maize ear rot is 15%;
In Comparative Examples 3, the sickness rate of Roots of Peanut maize ear rot is 13%;
After using commercially available common chemical fertilizer, the sickness rate of Roots of Peanut maize ear rot is 30%.
Embodiment 2
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, and Bacillus licheniformis strain is inoculated in disinfection fermentation tank, and fermention medium is: starch 0.2%, yeast extract paste 0.01%, dregs of beans 0.2%, urea 0.1%, dipotassium hydrogen phosphate 0.2%, potassium primary phosphate 0.1%, sal epsom 0.05%, manganous sulfate 0.006%, pH6.8, fermentation condition: ventilation ratio is initiated with 0.9,35 ℃ of culture temperature, stir revolution 100rpm, and dissolved oxygen is not less than 30%, the pH value is not less than 6.5, and stir culture 6 hours makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, and 45 ℃ of left and right forced air dryings of bacterium mud are to water content 25%, and cyclonic separation bacterium powder, put into pulverizer and pulverize, and crosses 30 mesh sieves and obtain the bacterium powder;
Bacillus laterosporus microbial inoculum preparation: the bacillus laterosporus bacterial classification is inoculated in disinfection fermentation tank, and fermention medium is: starch 0.2%, yeast extract paste 0.01%, dregs of beans 0.2%, urea 0.1%, dipotassium hydrogen phosphate 0.2%, potassium primary phosphate 0.1%, sal epsom 0.05%, manganous sulfate 0.006%, pH6.8, fermentation condition: ventilation ratio is initiated with 1:0.5, culture temperature 35, stir revolution 100rpm, dissolved oxygen is not less than 30%, pH value and is not less than 6.5, stir culture 6 hours, make viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, and 45 ℃ of forced air dryings of bacterium mud are to water content 25%, and cyclonic separation bacterium powder, put into pulverizer and pulverize, and crosses 30 mesh sieves and obtain the bacterium powder;
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, conidium liquid is proceeded in the PD liquid nutrient medium, shaking culture 3 days, then the part by weight according to 1:6 evenly mixes with rice bran or the wheat bran of sterilizing, be placed in the stainless steel mesh screen through liming or ozonization, the above covers the plastics film of sterilization, is placed in the indoor cultivation of 26 ℃ after 3 days, open plastics film, produce spore 4 days; Together with culture material, collect, the moon dries in the air 6 days, after putting into the pulverizer pulverizing, crosses 30 mesh sieves and makes the bacterium powder;
The preparation of complex microorganism bacteria agent:
The Bacillus licheniformis powder, bacillus laterosporus bacterium powder, the trichoderma harzianum bacterium powder that obtain in above step are mixed according to the ratio of 2:2:1, obtain the complex microorganism bacteria agent.
Get 5 parts of furfural dregs, 5 parts, ammonium sulfate, 5 parts of Secondary ammonium phosphates, 5 parts of vitriolate of tartar, 5 parts of potassium primary phosphates, above-mentioned raw material is mixed, under 90 ℃, dry, send into crusher in crushing powdered after, add the 1 part of mixing of complex microorganism bacteria agent prepared by above-mentioned step, obtain peanut specific complex microbial-bacterial fertilizer.
Embodiment 3
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, and Bacillus licheniformis strain is inoculated in disinfection fermentation tank, and fermention medium is: starch 0.8%, yeast extract paste 0.06%, dregs of beans 0.8%, urea 0.4%, dipotassium hydrogen phosphate 0.8%, potassium primary phosphate 0.5%, sal epsom 0.15%, manganous sulfate 0.012%, pH7.2, fermentation condition: ventilation ratio is initiated with 1:0.5,40 ℃ of culture temperature, stir revolution 200rpm, and dissolved oxygen is not less than 30%, the pH value is not less than 6.5, and stir culture 8 hours makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, and 50 ℃ of forced air dryings of bacterium mud are to water content 35%, and cyclonic separation bacterium powder, put into pulverizer and pulverize, and crosses 30 mesh sieves and obtain the bacterium powder;
Bacillus laterosporus microbial inoculum preparation: the bacillus laterosporus bacterial classification is inoculated in disinfection fermentation tank, fermention medium is: starch 0.8%, yeast extract paste 0.06%, dregs of beans 0.8%, urea 0.1-0.4%, dipotassium hydrogen phosphate 0.8%, potassium primary phosphate 0.5%, sal epsom 0.15%, manganous sulfate 0.012%, pH7.2, fermentation condition: ventilation ratio is initiated with 1:0.5,40 ℃ of culture temperature, stir revolution 200rpm, dissolved oxygen is not less than 30%, pH value and is not less than 6.5, stir culture 8 hours, make viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, and 50 ℃ of forced air dryings of bacterium mud are to water content 35%, and cyclonic separation bacterium powder, put into pulverizer and pulverize, and crosses 30 mesh sieves and obtain the bacterium powder;
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, conidium liquid is proceeded in the PD liquid nutrient medium, shaking culture 4 days, then the part by weight according to 1:10 evenly mixes with rice bran or the wheat bran of sterilizing, be placed in the stainless steel mesh screen through liming or ozonization, the above covers the plastics film of sterilization, is placed in the indoor cultivation of 30 ℃ after 5 days, open plastics film, produce spore 5 days; Together with culture material, collect, the moon dries in the air 7 days, after putting into the pulverizer pulverizing, crosses 30 mesh sieves and makes the bacterium powder;
The preparation of complex microorganism bacteria agent:
The Bacillus licheniformis powder, bacillus laterosporus bacterium powder, the trichoderma harzianum bacterium powder that obtain in above step are mixed according to the ratio of 2:2:1, obtain the complex microorganism bacteria agent.
Get 10 parts of furfural dregs, 25 parts, ammonium sulfate, 20 parts of Secondary ammonium phosphates, 20 parts of vitriolate of tartar, 20 parts of potassium primary phosphates, above-mentioned raw material is mixed, under 90 ℃, dry, send into crusher in crushing powdered after, add the 5 parts of mixing of complex microorganism bacteria agent that prepare by above-mentioned step, obtain peanut specific complex microbial-bacterial fertilizer.
Embodiment 4
With the difference of embodiment 1, be, in the present embodiment, also add some other the raw material that fertilizer efficiency is arranged, specific as follows: in the present embodiment, get 8 parts of furfural dregs, 16 parts, ammonium sulfate, 15 parts of Secondary ammonium phosphates, 15 parts of vitriolate of tartar, 15 parts of potassium primary phosphates, 10 parts, urea, 10 parts of peat composed of rotten mosses ashes, 15 parts of active sludge, 5 parts, straw powder, 15 parts of farm manures, above-mentioned raw material is mixed, under 90 ℃, dry, send into crusher in crushing powdered after, add the 3 parts of mixing of complex microorganism bacteria agent that prepare by above-mentioned step, obtain peanut specific complex microbial-bacterial fertilizer.
Embodiment 5
With the difference of embodiment 1, be, in the present embodiment, the raw material that adds some other auxiliary microbial growths and there is auxiliary disease-resistant effect, specific as follows: in the present embodiment, get 9 parts of furfural dregs, 15 parts, ammonium sulfate, 15 parts of Secondary ammonium phosphates, 15 parts of vitriolate of tartar, 15 parts of potassium primary phosphates, 10 parts, urea, 12 parts of dregs of beans, 14 parts of active sludge, 5 parts of chitins, 20 parts of farm manures, above-mentioned raw material is mixed, under 90 ℃, dry, send into crusher in crushing powdered after, add the 3 parts of mixing of complex microorganism bacteria agent that prepare by above-mentioned step, obtain peanut specific complex microbial-bacterial fertilizer.
Claims (7)
1. the specific complex microbial-bacterial fertilizer of cultivating peanut, is characterized in that, described fertilizer comprises the component of following portions by weight:
1～5 part of complex microorganism bacteria agent
5～10 parts of furfural dregs
5～25 parts, ammonium sulfate
5～20 parts of potassium primary phosphates
5～20 parts of Secondary ammonium phosphates
5～20 parts of vitriolate of tartar.
2. the as claimed in claim 1 one specific complex microbial-bacterial fertilizer of cultivating peanut, is characterized in that, described fertilizer comprises the component of following parts by weight:
2～4 parts of complex microorganism bacteria agents
6～8 parts of furfural dregs
10～20 parts, ammonium sulfate
10～16 parts of potassium primary phosphates
10～16 parts of Secondary ammonium phosphates
10～16 parts of vitriolate of tartar.
3. the as claimed in claim 1 one specific complex microbial-bacterial fertilizer of cultivating peanut, is characterized in that, described fertilizer comprises the component of following parts by weight:
3 parts of complex microorganism bacteria agents
8 parts of furfural dregs
16 parts, ammonium sulfate
15 parts of potassium primary phosphates
15 parts of Secondary ammonium phosphates
15 parts of vitriolate of tartar.
4. the as claimed in claim 1 one specific complex microbial-bacterial fertilizer of cultivating peanut, it is characterized in that, the preparation process of described complex microorganism bacteria agent is as follows: by Bacillus licheniformis, bacillus laterosporus and three independent inoculation fermentations of bacterial classifications difference of trichoderma harziarum, the lichen bacillus ferments liquid, bacillus laterosporus fermented liquid obtain Bacillus licheniformis powder, bacillus laterosporus powder through drying to pulverize respectively; Trichoderma harziarum zymophyte piece obtains the Trichoderma powder through drying in the shade, pulverizing, finally three kinds of bacterium powder are mixed, and obtains the complex microorganism bacteria agent.
5. the as claimed in claim 1 one specific complex microbial-bacterial fertilizer of cultivating peanut, is characterized in that, described composite bacillus microbial inoculum preparation process is specially:
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, Bacillus licheniformis strain is inoculated in disinfection fermentation tank, fermention medium is: starch 0.2-0.8%, yeast extract paste 0.01-0.06%, dregs of beans 0.2-0.8%, urea 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, sal epsom 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 35-40 ℃, stir revolution 100-200rpm, dissolved oxygen is not less than 30%, the pH value is not less than 6.5, stir culture 6-8 hour, make viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, 45-50 ℃ of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, put into pulverizer and pulverize, and crosses 30 mesh sieves and obtain the bacterium powder,
Bacillus laterosporus microbial inoculum preparation: the bacillus laterosporus bacterial classification is inoculated in disinfection fermentation tank, fermention medium is: starch 0.2-0.8%, yeast extract paste 0.01-0.06%, dregs of beans 0.2-0.8%, urea 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, sal epsom 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 35-40 ℃, stir revolution 100-200rpm, dissolved oxygen is not less than 30%, the pH value is not less than 6.5, stir culture 6-8 hour, make viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, 45-50 ℃ of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, put into pulverizer and pulverize, and crosses 30 mesh sieves and obtain the bacterium powder,
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, conidium liquid is proceeded in the PD liquid nutrient medium, shaking culture 2-4 days, then the part by weight according to 1:6-10 evenly mixes with rice bran or the wheat bran of sterilizing, be placed in the stainless steel mesh screen through liming or ozonization, the above covers the plastics film of sterilization, after being placed in 22-30 ℃ indoor cultivation 3-5 days, open plastics film, produce spore 3-5 days; Together with culture material, collect, the moon 5-7 days that dries in the air, after putting into pulverizer and pulverizing, cross 30 mesh sieves and make the bacterium powder;
The preparation of complex microorganism bacteria agent:
The Bacillus licheniformis powder, bacillus laterosporus bacterium powder, the trichoderma harzianum bacterium powder that obtain in above-mentioned steps are mixed according to the ratio of 1-4:1-4:1, obtain the complex microorganism bacteria agent.
6. as described as claim 1 or 5 one preparation method who cultivates peanut the specific complex microbial-bacterial fertilizer, is characterized in that,
Get 5～10 parts of furfural dregs, 5～25 parts, ammonium sulfate, 5～20 parts of potassium primary phosphates, 5～20 parts of Secondary ammonium phosphates, 5～20 parts of vitriolate of tartar, above-mentioned raw material is mixed, under 90 ℃, dry, send into crusher in crushing powdered after, add 1～5 part of mixing of complex microorganism bacteria agent, obtain peanut specific complex microbial-bacterial fertilizer.
7. as described as claim 1 or 6 one preparation method who cultivates peanut the specific complex microbial-bacterial fertilizer, is characterized in that, described composite bacillus microbial inoculum preparation process is specially:
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, and Bacillus licheniformis strain is inoculated in disinfection fermentation tank, and fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, sal epsom 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5,37 ℃ of culture temperature, stir revolution 150rpm, and dissolved oxygen is not less than 30%, the pH value is not less than 6.5, and stir culture 7 hours makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, bacterium mud is in 45 ℃ of lower forced air dryings to water content 30%, cyclonic separation bacterium powder, put into pulverizer and pulverized, and crosses 30 mesh sieves and obtain the bacterium powder;
Bacillus laterosporus microbial inoculum preparation: the bacillus laterosporus bacterial classification is inoculated in disinfection fermentation tank, and fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, sal epsom 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5,37 ℃ of culture temperature, stir revolution 150rpm, dissolved oxygen is not less than 30%, pH value and is not less than 6.5, stir culture 7 hours, make viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, and the rear employing filter press mode of having flocculated obtains bacterium mud, bacterium mud is in 45 ℃ of lower forced air dryings to water content 30%, cyclonic separation bacterium powder, put into pulverizer and pulverized, and crosses 30 mesh sieves and obtain the bacterium powder;
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, cultural method is liquid rotating solid biphasic cultivation method, conidium liquid is proceeded in the PD liquid nutrient medium, shaking culture 3 days, then the part by weight according to 1:8 evenly mixes with rice bran or the wheat bran of sterilizing, is placed in the stainless steel mesh screen through liming or ozonization, the above covers the plastics film of sterilization, be placed under 28 ℃ and after 4 days, open plastics film at indoor cultivation, produce spore 4 days; Together with culture material, collect, the moon dries in the air 6 days, after putting into the pulverizer pulverizing, crosses 30 mesh sieves and makes the bacterium powder;
The complex micro organism fungicide preparation:
The Bacillus licheniformis powder, bacillus laterosporus bacterium powder, the trichoderma harzianum bacterium powder that obtain in above step are mixed according to the ratio of 2:2:1, obtain the complex microorganism bacteria agent.
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| CN103848702A (en) * | 2014-01-14 | 2014-06-11 | 淅川县绿新生物科技有限公司 | Fertilizer for peanuts |
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| CN105948992A (en) * | 2016-05-14 | 2016-09-21 | 安徽新天地生物肥业有限公司 | Special organic slow release fertilizer for peanuts |
| CN106342858A (en) * | 2016-08-26 | 2017-01-25 | 安徽久易农业股份有限公司 | Post-emergence compound herbicide for wheat fields |
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| CN112457138A (en) * | 2020-12-21 | 2021-03-09 | 东北农业大学 | Microbial preparation for improving soil fertility of saline-alkali soil and preparation method thereof |
| CN112679256A (en) * | 2021-01-13 | 2021-04-20 | 上海市环境科学研究院 | Preparation method of straw biogas residue-based composite microbial fertilizer |
| CN113303316A (en) * | 2021-06-16 | 2021-08-27 | 茂生生物科技保定有限公司 | High-efficiency sterilization and disinfection method for seedbed soil |
| CN113303316B (en) * | 2021-06-16 | 2023-02-17 | 茂生生物科技保定有限公司 | High-efficiency sterilization and disinfection method for seedbed soil |
| CN114394863A (en) * | 2022-01-18 | 2022-04-26 | 山东省农业科学院 | Microbial water-soluble fertilizer for preventing and treating peanut diseases and insect pests and preparation method thereof |
| CN115259963A (en) * | 2022-06-23 | 2022-11-01 | 中盐安徽红四方肥业股份有限公司 | Organic-inorganic compound fertilizer containing functional bacteria and production method thereof |
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| CN103483073B (en) | 2015-01-07 |
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Effective date of registration: 20220908 Address after: Room 607, North Building, No. 29, Meiling Road, Laoshan District, Qingdao City, Shandong Province, 266000 Patentee after: Deep Blue Grapefruit Fertilizer Industry (Qingdao) Co.,Ltd. Address before: North of Gongren Road, Jiangshan Industrial Park, Laixi City, Qingdao City, Shandong Province 266603 Patentee before: QINGDAO SHENLAN FERTILIZER INDUSTRY CO.,LTD. |
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