CN112616857A - Liquid compound microorganism rooting agent for improving saline-alkali soil and application thereof - Google Patents
Liquid compound microorganism rooting agent for improving saline-alkali soil and application thereof Download PDFInfo
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Abstract
The invention discloses a liquid compound microorganism rooting agent for improving saline-alkali soil and application thereof. The raw materials comprise the following components in parts by weight: 5-10 parts of composite microbial inoculum, 5-10 parts of brown sugar, 5-10 parts of monocalcium phosphate, 3-5 parts of monopotassium phosphate, 65-87 parts of soluble starch and 1000 parts of clean water 850-. The rooting agent of the invention is mixed with crop seeds evenly and then sown. Or diluting the rooting agent with clear water, soaking the root of the seedling, and then transplanting the seedling. The using amount of the rooting agent is 1-2L of seed dressing 500kg, and 1.5L of seedlings with 1 hectare are soaked. The invention can reduce the salt content and pH value of the saline-alkali soil, promote the rooting and growth of crops, increase the yield of the crops and improve the soil structure of the saline-alkali soil.
Description
Technical Field
The invention belongs to the technical field of saline-alkali soil improvement, and particularly relates to a liquid compound microorganism rooting agent for improving saline-alkali soil and application thereof.
Background
The soil salinization process comprises two different soil-forming processes of salinization and alkalization. The salinization process is usually NaCl, CaCl2、Na2SO4、MgSO4The accumulation process of neutral or near-neutral salts in the surface layer and the soil body is equal, so that the soil is in neutral or alkaline reaction. The salinization of soil is not beneficial to the growth of plants, brings great pressure to the stability of ecological environment, and brings serious harm to the environment and economic development. The area of the saline-alkali soil in China is one fifth of that of cultivated land, so that the improvement and development of the saline-alkali soil have great significance for the sustainable development of economy in China.
Among various saline-alkali soil improvement measures, the biological improvement saline-alkali soil has the characteristics of being eco-friendly and continuous and stable, and the microbial fertilizer is applied to crops planted in the saline-alkali soil to promote the growth of the crops, so that the improvement of the saline-alkali soil is of great significance.
Disclosure of Invention
The invention aims to provide a liquid compound microorganism rooting agent for improving saline-alkali soil and application thereof, which are used for promoting crops planted in the saline-alkali soil to root and grow so as to reduce the salt content and the pH value of the saline-alkali soil.
On one hand, the invention provides a liquid compound microorganism rooting agent for improving saline-alkali soil, which is prepared from raw materials consisting of a compound microbial inoculum, brown sugar, monocalcium phosphate, monopotassium phosphate, soluble starch and clear water.
Preferably, the liquid composite microorganism rooting agent is prepared by uniformly mixing the raw materials and then carrying out facultative fermentation at the temperature of 22-28 ℃ for 48 hours.
Preferably, the raw materials comprise, by weight, 5-10 parts of the complex microbial inoculum, 5-10 parts of brown sugar, 5-10 parts of monocalcium phosphate, 3-5 parts of monopotassium phosphate, 65-87 parts of soluble starch and 1000 parts of clear water.
Preferably, the raw materials comprise, by weight, 5 parts of the complex microbial inoculum, 6 parts of brown sugar, 7 parts of calcium dihydrogen phosphate, 3 parts of potassium dihydrogen phosphate, 71 parts of soluble starch and 850 parts of clean water.
Preferably, the raw materials comprise 7 parts of the complex microbial inoculum, 8 parts of brown sugar, 10 parts of calcium dihydrogen phosphate, 4 parts of potassium dihydrogen phosphate, 80 parts of soluble starch and 900 parts of clean water by weight.
Preferably, the raw materials comprise, by weight, 9 parts of the complex microbial inoculum, 10 parts of brown sugar, 8 parts of calcium dihydrogen phosphate, 5 parts of potassium dihydrogen phosphate, 65 parts of soluble starch and 950 parts of clear water.
Preferably, the raw materials comprise, by weight, 10 parts of the complex microbial inoculum, 5 parts of brown sugar, 9 parts of calcium dihydrogen phosphate, 4 parts of potassium dihydrogen phosphate, 87 parts of soluble starch and 1000 parts of clear water.
Preferably, the complex microbial inoculum comprises aspergillus glaucous, trichoderma harzianum, verticillium chlamydosporium, aspergillus oryzae, lactobacillus rhamnosus and saccharomyces cerevisiae.
Preferably, the content of the Aspergillus glaucopiae is more than or equal to 3.0 x 109cfu/g, the bacterium content of the trichoderma harzianum is more than or equal to 2.0 multiplied by 109cfu/g, the bacterial content of the verticillium chlamydosporium is more than or equal to 1.0 multiplied by 109cfu/g, the Aspergillus oryzae containing strainThe amount is more than or equal to 1.5 multiplied by 1010cfu/g, the bacterium content of the lactobacillus rhamnosus is more than or equal to 1.0 multiplied by 1011cfu/g, the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 109cfu/g。
On the other hand, the invention also provides the application of the liquid compound microorganism rooting agent, before crops are planted on the saline-alkali soil, the liquid compound microorganism rooting agent and crop seeds are uniformly mixed and stirred, and then sown after being dried in the shade, or the liquid compound microorganism rooting agent is diluted by clear water and then the roots of seedlings are soaked, then the seedlings are transplanted, 500kg of the liquid compound microorganism rooting agent is mixed for every 1-2L, and 1 hectare of the seedlings are soaked by every 1.5L of the liquid compound microorganism rooting agent.
The liquid compound microorganism rooting agent can promote the rooting and growth of crops planted in saline-alkali soil, thereby reducing the salt content and pH value of the saline-alkali soil and improving the soil structure and ecological environment of the saline-alkali soil.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
The invention mixes brown sugar, monocalcium phosphate, monopotassium phosphate, soluble starch and clear water, and adopts beneficial microorganisms to ferment and prepare the liquid compound microorganism rooting agent for improving saline-alkali soil. The rooting agent is sprayed in saline-alkali soil, and can promote the rooting and growth of crops planted in the saline-alkali soil, so that the salt content and the pH value of the saline-alkali soil are reduced, and the soil structure and the ecological environment of the saline-alkali soil are improved.
In a specific embodiment of the invention, the liquid compound microorganism rooting agent for improving saline-alkali soil is prepared from raw materials consisting of a compound microbial inoculum, brown sugar, monocalcium phosphate, monopotassium phosphate, soluble starch and clear water. The specific preparation process comprises the following steps: step 1, preparing a complex microbial inoculum; step 2, preparing a mixed material; and 3, fermenting the mixed material.
In the step 1 of preparing the composite microbial inoculum, Aspergillus glaucus powder, Trichoderma harzianum powder, Verticillium chlamydosporium powder, Aspergillus oryzae powder, Lactobacillus rhamnosus powder and Saccharomyces cerevisiae powder are selected, and then the six kinds of the bacteria are compounded according to the proportioning relationship by weight to obtain the composite microbial inoculum, wherein 8-15 parts of the microbial inoculum is preferably selected, 15-25 parts of the Trichoderma harzianum powder, 10-20 parts of the Verticillium chlamydosporium powder, 5-15 parts of the Aspergillus oryzae powder, 15-25 parts of the Lactobacillus rhamnosus powder and 10-20 parts of the Saccharomyces cerevisiae powder. On one hand, lactobacillus rhamnosus and saccharomyces cerevisiae are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then thalli are separated from the fermentation culture and dried and concentrated to prepare single-strain solid bacterial powder. The bacterial content of each single bacterial strain powder is respectively as follows: the content of Lactobacillus rhamnosus powder is not less than 1.0 × 1011The bacterium content of cfu/g and the saccharomyces cerevisiae powder is more than or equal to 4.0 multiplied by 109cfu/g. On the other hand, aspergillus glaucus, trichoderma harzianum, verticillium chlamydosporium and aspergillus oryzae are fermented in corresponding solid culture media respectively to obtain solid cultures, and then the solid cultures are dried and crushed to prepare single-strain solid bacterial powder. The bacterial content of each single bacterial strain powder is respectively as follows: the content of Aspergillus glaucus powder is not less than 3.0 × 109The bacterial content of cfu/g and trichoderma harzianum powder is more than or equal to 2.0 multiplied by 109cfu/g, the bacterial content of verticillium chlamydosporium powder is more than or equal to 1.0 multiplied by 109The bacterial content of cfu/g and Aspergillus oryzae is more than or equal to 1.5 multiplied by 1010cfu/g。
In the step 2 of preparing the mixed material, various raw material components are weighed according to a preset weight ratio, and then all the raw materials are mixed and stirred uniformly. The weight ratio of each component in the raw materials is respectively optimized as follows: 5-10 parts of composite microbial inoculum, 5-10 parts of brown sugar, 5-10 parts of monocalcium phosphate, 3-5 parts of monopotassium phosphate, 65-87 parts of soluble starch and 1000 parts of clean water 850-.
In the step 3 of fermenting the mixed material, the initial pH of the mixed material is natural, then the mixed material is subjected to facultative fermentation for 48 hours at the temperature of 22-28 ℃, and the fermented product is used as a liquid compound microorganism rooting agent.
The invention also provides the application of the liquid compound microorganism rooting agent, before crops are planted in the saline-alkali soil, the liquid compound microorganism rooting agent and crop seeds are uniformly mixed and stirred, and then sown after being dried in the shade, or the liquid compound microorganism rooting agent is diluted by clear water and then the roots of seedlings are soaked, then the seedlings are transplanted, 500kg of the liquid compound microorganism rooting agent is mixed with 1-2L of the seeds, and 1.5L of the liquid compound microorganism rooting agent is soaked in 1 hectare of seedlings.
To help better understand the technical scheme of the invention, the following examples are provided for illustrating the preparation process and the application method of the liquid composite microorganism rooting agent of the invention.
Example one
The liquid composite microorganism rooting agent comprises, by weight, 5 parts of a composite microbial inoculum, 6 parts of brown sugar, 7 parts of monocalcium phosphate, 3 parts of monopotassium phosphate, 71 parts of soluble starch and 850 parts of clear water. The composite microbial agent is prepared by mixing 8 parts of aspergillus glaucus powder, 15 parts of trichoderma harzianum powder, 20 parts of verticillium chlamydosporium powder, 5 parts of aspergillus oryzae powder, 18 parts of lactobacillus rhamnosus powder and 16 parts of saccharomyces cerevisiae powder. The bacteria content of the six bacteria powder is respectively as follows: the bacteria content of Aspergillus glaucus is not less than 3.0 × 109The bacterial content of cfu/g and trichoderma harzianum is more than or equal to 2.0 multiplied by 109cfu/g, content of Verticillium chlamydosporia is more than or equal to 1.0 x 109cfu/g, the bacteria content of Aspergillus oryzae is more than or equal to 1.5 multiplied by 1010cfu/g, the bacterium content of lactobacillus rhamnosus is more than or equal to 1.0 multiplied by 1011cfu/g and the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 109cfu/g。
The liquid compound microorganism rooting agent is prepared by the following steps.
Step 1, preparing a composite microbial inoculum, and uniformly mixing aspergillus glaucus powder, trichoderma harzianum powder, verticillium chlamydosporium powder, aspergillus oryzae powder, lactobacillus rhamnosus powder and saccharomyces cerevisiae powder to obtain the composite microbial inoculum.
And 2, mixing the raw materials to prepare a mixed material, adding the composite microbial inoculum, brown sugar, monocalcium phosphate, monopotassium phosphate, soluble starch and clear water, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, then carrying out facultative fermentation for 48 hours at the temperature of 22-28 ℃, and taking the fermented product as the liquid compound microorganism rooting agent 1.
The application method of the liquid compound microorganism rooting agent 1 comprises the following steps: before planting crops on a saline-alkali land, 1L of the liquid compound microorganism rooting agent 1 and 500kg of crop seeds are uniformly mixed and stirred, and the mixture is dried in the shade and then sowed, or 1.5L of the liquid compound microorganism rooting agent 1 is diluted by clear water and then soaked in the roots of 1 hectare of seedlings, and then the seedlings are transplanted.
Example two
The liquid composite microorganism rooting agent comprises 7 parts by weight of composite microbial inoculum, 8 parts by weight of brown sugar, 10 parts by weight of monocalcium phosphate, 4 parts by weight of monopotassium phosphate, 80 parts by weight of soluble starch and 900 parts by weight of clear water. The composite microbial inoculum is prepared by mixing 11 parts of aspergillus glaucus powder, 25 parts of trichoderma harzianum powder, 17 parts of verticillium chlamydosporium powder, 10 parts of aspergillus oryzae powder, 21 parts of lactobacillus rhamnosus powder and 14 parts of saccharomyces cerevisiae powder. The bacteria content of the six bacteria powder is respectively as follows: the bacteria content of Aspergillus glaucus is not less than 3.0 × 109The bacterial content of cfu/g and trichoderma harzianum is more than or equal to 2.0 multiplied by 109cfu/g, content of Verticillium chlamydosporia is more than or equal to 1.0 x 109cfu/g, the bacteria content of Aspergillus oryzae is more than or equal to 1.5 multiplied by 1010cfu/g, the bacterium content of lactobacillus rhamnosus is more than or equal to 1.0 multiplied by 1011cfu/g and the bacterial content of Saccharomyces cerevisiae≥4.0×109cfu/g。
The liquid compound microorganism rooting agent is prepared by the following steps.
Step 1, preparing a composite microbial inoculum, and uniformly mixing aspergillus glaucus powder, trichoderma harzianum powder, verticillium chlamydosporium powder, aspergillus oryzae powder, lactobacillus rhamnosus powder and saccharomyces cerevisiae powder to obtain the composite microbial inoculum.
And 2, mixing the raw materials to prepare a mixed material, adding the composite microbial inoculum, brown sugar, monocalcium phosphate, monopotassium phosphate, soluble starch and clear water, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, then carrying out facultative fermentation for 48 hours at the temperature of 22-28 ℃, and taking a fermented product as a liquid compound microorganism rooting agent 2.
The application method of the liquid compound microorganism rooting agent 2 comprises the following steps: before planting crops on the saline-alkali soil, 1.3L of the liquid compound microorganism rooting agent 2 and 500kg of crop seeds are uniformly mixed and stirred, and the mixture is dried in the shade and then sowed, or 1.5L of the liquid compound microorganism rooting agent 2 is diluted by clear water and then soaked in the roots of 1 hectare of seedlings, and then the seedlings are transplanted.
EXAMPLE III
The liquid composite microorganism rooting agent comprises 9 parts by weight of composite microbial inoculum, 10 parts by weight of brown sugar, 8 parts by weight of monocalcium phosphate, 5 parts by weight of monopotassium phosphate, 65 parts by weight of soluble starch and 950 parts by weight of clear water. The composite microbial agent is prepared by mixing 13 parts of aspergillus glaucus powder, 22 parts of trichoderma harzianum powder, 14 parts of verticillium chlamydosporium powder, 15 parts of aspergillus oryzae powder, 15 parts of lactobacillus rhamnosus powder and 20 parts of saccharomyces cerevisiae powder. The bacteria content of the six bacteria powder is respectively as follows: the bacteria content of Aspergillus glaucus is not less than 3.0 × 109The bacterial content of cfu/g and trichoderma harzianum is more than or equal to 2.0 multiplied by 109cfu/g, content of Verticillium chlamydosporia is more than or equal to 1.0 x 109cfu/g, the bacteria content of Aspergillus oryzae is more than or equal to 1.5 multiplied by 1010cfu/g, content of Lactobacillus rhamnosus≥1.0×1011cfu/g and the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 109cfu/g。
The liquid compound microorganism rooting agent is prepared by the following steps.
Step 1, preparing a composite microbial inoculum, and uniformly mixing aspergillus glaucus powder, trichoderma harzianum powder, verticillium chlamydosporium powder, aspergillus oryzae powder, lactobacillus rhamnosus powder and saccharomyces cerevisiae powder to obtain the composite microbial inoculum.
And 2, mixing the raw materials to prepare a mixed material, adding the composite microbial inoculum, brown sugar, monocalcium phosphate, monopotassium phosphate, soluble starch and clear water, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, then carrying out facultative fermentation for 48 hours at the temperature of 22-28 ℃, and taking a fermented product as a liquid compound microorganism rooting agent 3.
The application method of the liquid compound microorganism rooting agent 3 comprises the following steps: before planting crops on the saline-alkali soil, 1.6L of the liquid compound microorganism rooting agent 3 and 500kg of crop seeds are uniformly mixed and stirred, and the mixture is dried in the shade and then sowed, or 1.5L of the liquid compound microorganism rooting agent 3 is diluted by clear water and then soaked in the roots of 1 hectare of seedlings, and then the seedlings are transplanted.
Example four
The liquid composite microorganism rooting agent comprises, by weight, 10 parts of a composite microbial inoculum, 5 parts of brown sugar, 9 parts of monocalcium phosphate, 4 parts of monopotassium phosphate, 87 parts of soluble starch and 1000 parts of clear water. The composite microbial agent is prepared by mixing 15 parts of aspergillus glaucus powder, 18 parts of trichoderma harzianum powder, 10 parts of verticillium chlamydosporium powder, 13 parts of aspergillus oryzae powder, 25 parts of lactobacillus rhamnosus powder and 10 parts of saccharomyces cerevisiae powder. The bacteria content of the six bacteria powder is respectively as follows: the bacteria content of Aspergillus glaucus is not less than 3.0 × 109The bacterial content of cfu/g and trichoderma harzianum is more than or equal to 2.0 multiplied by 109cfu/g, content of Verticillium chlamydosporia is more than or equal to 1.0 x 109cfu/g, and the bacteria content of Aspergillus oryzae is not less than1.5×1010cfu/g, the bacterium content of lactobacillus rhamnosus is more than or equal to 1.0 multiplied by 1011cfu/g and the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 109cfu/g。
The liquid compound microorganism rooting agent is prepared by the following steps.
Step 1, preparing a composite microbial inoculum, and uniformly mixing aspergillus glaucus powder, trichoderma harzianum powder, verticillium chlamydosporium powder, aspergillus oryzae powder, lactobacillus rhamnosus powder and saccharomyces cerevisiae powder to obtain the composite microbial inoculum.
And 2, mixing the raw materials to prepare a mixed material, adding the composite microbial inoculum, brown sugar, monocalcium phosphate, monopotassium phosphate, soluble starch and clear water, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, then carrying out facultative fermentation for 48 hours at the temperature of 22-28 ℃, and taking a fermented product as a liquid compound microorganism rooting agent 4.
The application method of the liquid compound microorganism rooting agent 4 comprises the following steps: before planting crops on the saline-alkali soil, 2.0L of the liquid compound microorganism rooting agent 4 and 500kg of crop seeds are uniformly mixed and stirred, and the mixture is dried in the shade and then sowed, or 1.5L of the liquid compound microorganism rooting agent 4 is diluted by clear water and then soaked in the roots of 1 hectare of seedlings, and then the seedlings are transplanted.
In order to help better understand the technical scheme of the invention, a test example of crop planting is provided below for illustrating the application effect of the invention.
The first test example: effect of liquid compound microorganism rooting agent on saline-alkali soil improvement and influence on rice growth
The same saline-alkali field block is selected from Ningxia Yongning, the basic physicochemical property of field soil is pH value 8.65, the total salt content of the soil of a plough layer is 6.25g/kg, the alkalization degree is 24.31%, the organic matter content is 15.84g/kg, the total nitrogen content is 1.07g/kg, the quick-acting phosphorus content is 18.03mg/kg, the quick-acting potassium content is 179mg/kg, and the alkaline hydrolysis nitrogen content is 28.15 mg/kg. 5 groups of experimental designs, including 4 experimental groups and 1 control group, each group of experimental designs 3 experimental cells, each area of 30m2All test cells are randomly distributed.
Test ofFertilizer and the liquid compound microorganism rooting agent prepared by the invention are applied in groups. The base fertilizer is applied with urea, calcium superphosphate and potassium sulfate, and the fertilizing amount of the three fertilizers is 138kg/hm of urea respectively2Calcium superphosphate 95kg/hm2And potassium sulfate 95kg/hm2. Applying urea once at seedling stage, tillering stage and jointing stage of rice, wherein the urea applying amount is 20kg/hm240kg/hm at tillering stage2And the jointing-pulling booting period is 20kg/hm2. Before transplanting rice seedlings, diluting the liquid compound microorganism rooting agent by 10 times with clear water, soaking the roots of the rice seedlings for 30min, and then transplanting rice seedlings. The dosage of the liquid compound microorganism rooting agent is 1hm per 1.5L of the liquid compound microorganism rooting agent2The seedlings are obtained. The control group uses the same amount of clear water to replace the liquid compound microorganism rooting agent, and the rest management is the same as the test group.
Donggao No. 4 is selected as a test variety, transplanting is carried out in the middle ten days of 4 months according to the mode of 17cm of plant spacing, 30cm of row spacing and 5 seedlings per hole, the same conventional management is adopted in each cell, and harvesting is carried out in the last ten days of 9 months. When the rice seedlings are transplanted for 10 days, randomly selecting 5 points in each cell, selecting 3 rice seedlings in each point, measuring the plant height, root length, root number and underground fresh weight of each rice seedling, and calculating the average plant height, average root length, average root number and average underground fresh weight of each group of rice seedlings. The results are shown in Table 1.
TABLE 1
The data in table 1 show that the four groups of rice seedlings applied with the liquid compound microorganism rooting agent are obviously higher than those of the control group in four properties of plant height, root length, root number and underground fresh weight. Therefore, the prepared liquid compound microorganism rooting agent 1-liquid compound microorganism rooting agent 4 can obviously promote the rooting of rice seedlings, thereby promoting the growth of rice plants.
After harvesting the rice, the yield of each cell is counted, and the average cell yield of each group is calculated. And simultaneously, taking a soil sample to measure the pH, the total salt content and the alkalization degree of the soil in each cell, and calculating the average pH, the average total salt content and the average alkalization degree of each group of soil. The results are shown in Table 2.
TABLE 2
As can be seen from the data in Table 2, the pH, the total salt content and the alkalization degree of the rice planting soil of the 4 groups applied with the liquid compound microorganism rooting agent are all obviously lower than those of the control, and the rice yield of the plot is obviously higher than that of the control. Therefore, the prepared liquid compound microorganism rooting agent 1-liquid compound microorganism rooting agent 4 can obviously improve saline-alkali soil, reduce the pH value and the salt content of the soil, promote the growth of rice and improve the yield of the rice.
Test example two: effect of liquid compound microorganism rooting agent on saline-alkali soil improvement and influence on growth of sweet sorghum
The same saline-alkali field block is selected in Ningxia Yinchuan, the basic physicochemical property of the field soil is that the pH value is 8.33, the total salt content of the soil of a plough layer is 3.11g/kg, the alkalization degree is 18.2%, the organic matter content is 18.16g/kg, the total nitrogen content is 1.10g/kg, the quick-acting phosphorus content is 6.54mg/kg, the quick-acting potassium content is 143mg/kg, and the alkaline hydrolysis nitrogen content is 55.12 mg/kg. The experimental design 5 groups comprise 4 experimental groups and 1 control group, each group of experimental designs comprises 3 experimental cells, and the area of each experimental cell is 20m2All test cells are randomly distributed.
The test group is applied with fertilizer and the liquid compound microorganism rooting agent prepared by the invention. Urea, diamine phosphate and potassium sulfate are applied to the base fertilizer, and the fertilizing amount of the three fertilizers is 600kg/hm of urea respectively2375kg/hm diammonium phosphate2And potassium sulfate 300kg/hm2. The liquid compound microorganism rooting agent and the sweet sorghum seeds are applied in a seed dressing way. Mixing liquid composite microorganism rooting agent and seeds according to a certain proportion, drying the seeds in the shade, and sowing, wherein each 500kg of sweet sorghumThe seed uses 4 rooting agents in the following amounts: 1L of liquid compound microorganism rooting agent 1, 1.3L of liquid compound microorganism rooting agent 2, 1.6L of liquid compound microorganism rooting agent 3 and 2.0L of liquid compound microorganism rooting agent 4. The control group uses the same amount of clear water to replace the liquid compound microorganism rooting agent, and the rest management is the same as the test group.
Selecting Liaotian No. 3 as a test variety, sowing in a hill-drop manner in the last ten days of 4 months, and sowing 4-78 seeds in each hill with a plant spacing of 20cm and a row spacing of 45 cm. The same general management is adopted for each cell, and the cells are harvested in the last 9 months. When the seedlings of the sweet sorghum emerge for 45 days, 5 seedlings are taken from each cell and washed clean, the plant height, the root length, the dry weight of the overground part and the dry weight of the root system are measured, and the average plant height, the average root length, the average dry weight of the overground part and the average dry weight of the root system of each group are calculated. The results are shown in Table 3.
TABLE 3
As can be seen from the data in Table 3, the 4 groups of sweet sorghum seedlings to which the liquid complex microorganism rooting agent is applied are obviously higher than the control group in four shapes of plant height, root length, dry weight of aerial parts and dry weight of root systems. Therefore, the prepared liquid composite microorganism rooting agent 1-liquid composite microorganism rooting agent 4 can obviously promote the rooting of sweet sorghum seedlings, so that the growth of sweet sorghum plants is promoted.
After the sweet sorghum is harvested, the yield of each cell is counted, and the average cell yield of each group is calculated. And simultaneously, taking a soil sample to measure the pH, the total salt content and the alkalization degree of the soil in each cell, and calculating the average pH, the average total salt content and the average alkalization degree of each group of soil. The results are shown in Table 4.
TABLE 4
As can be seen from the data in Table 4, the pH, the total salt content and the alkalization degree of the sweet sorghum planting soil of 4 groups applied with the liquid compound microorganism rooting agent are all obviously lower than those of the control, and the yield of the sweet sorghum in the cell is obviously higher than that of the control. Therefore, the prepared liquid composite microorganism rooting agent 1-the liquid composite microorganism rooting agent 4 can obviously improve saline-alkali soil, reduce the pH value and the salt content of the soil, promote the growth of the sweet sorghum and improve the yield of the sweet sorghum.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.
Claims (10)
1. A liquid compound microorganism rooting agent for improving saline-alkali soil is prepared from a compound microbial inoculum, brown sugar, monocalcium phosphate, monopotassium phosphate, soluble starch and clear water.
2. The liquid composite microbial rooting agent according to claim 1, wherein: the liquid compound microorganism rooting agent is prepared by uniformly mixing the raw materials and then carrying out facultative fermentation for 48 hours at the temperature of 22-28 ℃.
3. The liquid composite microbial rooting agent according to claim 1, wherein: the raw materials comprise, by weight, 5-10 parts of the complex microbial inoculum, 5-10 parts of brown sugar, 5-10 parts of monocalcium phosphate, 3-5 parts of monopotassium phosphate, 65-87 parts of soluble starch and 1000 parts of clean water 850-.
4. The liquid composite microbial rooting agent according to claim 3, wherein: the raw materials comprise, by weight, 5 parts of the complex microbial inoculum, 6 parts of brown sugar, 7 parts of monocalcium phosphate, 3 parts of monopotassium phosphate, 71 parts of soluble starch and 850 parts of clear water.
5. The liquid composite microbial rooting agent according to claim 3, wherein: the raw materials comprise 7 parts of the compound microbial inoculum, 8 parts of brown sugar, 10 parts of monocalcium phosphate, 4 parts of monopotassium phosphate, 80 parts of soluble starch and 900 parts of clear water by weight.
6. The liquid composite microbial rooting agent according to claim 3, wherein: the raw materials comprise, by weight, 9 parts of the complex microbial inoculum, 10 parts of brown sugar, 8 parts of monocalcium phosphate, 5 parts of monopotassium phosphate, 65 parts of soluble starch and 950 parts of clear water.
7. The liquid composite microbial rooting agent according to claim 3, wherein: the raw materials comprise, by weight, 10 parts of the complex microbial inoculum, 5 parts of brown sugar, 9 parts of monocalcium phosphate, 4 parts of monopotassium phosphate, 87 parts of soluble starch and 1000 parts of clear water.
8. The liquid composite microorganism rooting agent according to any one of claims 1 to 7, characterized in that: the complex microbial inoculum comprises aspergillus glaucus, trichoderma harzianum, verticillium chlamydosporium, aspergillus oryzae, lactobacillus rhamnosus and saccharomyces cerevisiae.
9. The liquid composite microbial rooting agent according to claim 8, wherein: the content of the Aspergillus glaucus is more than or equal to 3.0 multiplied by 109cfu/g, the bacterium content of the trichoderma harzianum is more than or equal to 2.0 multiplied by 109cfu/g, the bacterial content of the verticillium chlamydosporium is more than or equal to 1.0 multiplied by 109cfu/g, the bacterium content of the aspergillus oryzae is more than or equal to 1.5 multiplied by 1010cfu/g, the bacterium content of the lactobacillus rhamnosus is more than or equal to 1.0 multiplied by 1011cfu/g, the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 109cfu/g。
10. The use of the liquid composite microorganism rooting agent according to any one of claims 1 to 9, wherein: before crops are planted on the saline-alkali land, the liquid compound microorganism rooting agent and crop seeds are uniformly mixed and stirred, and the mixture is dried in the shade and then sown, or the liquid compound microorganism rooting agent is diluted by clear water and then the roots of seedlings are soaked, then the seedlings are transplanted, 500kg of the liquid compound microorganism rooting agent is mixed for every 1-2L of the seedlings, and 1 hectare of the seedlings are soaked by the liquid compound microorganism rooting agent for every 1.5L of the seedlings.
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