CN107935672A - A kind of salt-soda soil growth-promoting bio-bacterial manure - Google Patents

A kind of salt-soda soil growth-promoting bio-bacterial manure Download PDF

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CN107935672A
CN107935672A CN201711009804.8A CN201711009804A CN107935672A CN 107935672 A CN107935672 A CN 107935672A CN 201711009804 A CN201711009804 A CN 201711009804A CN 107935672 A CN107935672 A CN 107935672A
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王胜
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Shandong Scenic Tourism Development Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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    • C05—FERTILISERS; MANUFACTURE THEREOF
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    • C05B13/00—Fertilisers produced by pyrogenic processes from phosphatic materials
    • C05B13/02—Fertilisers produced by pyrogenic processes from phosphatic materials from rock phosphates
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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Abstract

The present invention relates to bio-bacterial manure technical field, more particularly to a kind of salt-soda soil growth-promoting bio-bacterial manure.Including A：Complex micro organism fungicide produces；B：Growth-promoting bio-bacterial manure：By complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, concave convex rod be stirred in proportion 1.5 2 it is small when, through be granulated, dry, sieve be made salt-soda soil growth-promoting bio-bacterial manure；Whereby, by that will pass through liquid fermentation and solid fermentation trichoderma asperellum solid spawn, bacillus subtilis solid spawn and bacillus licheniformis solid spawn is made and by it by 1 in the present invention:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；Then by weight turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, concave convex rod is added, then it is stirred, is granulated, drying, sieving obtained salt-soda soil growth-promoting bio-bacterial manure.The bio-bacterial manure is subjected to the salt-soda soil plantation fluffy Biological control of alkali, fluffy survival rate >=95% of alkali.

Description

A kind of salt-soda soil growth-promoting bio-bacterial manure
Technical field
The present invention relates to bio-bacterial manure technical field, more particularly to a kind of salt-soda soil growth-promoting bio-bacterial manure.
Background technology
Soil salinization problem increasingly threatens limited land resource for the survival of mankind.In population increase, arable land Increasingly reduce and the severe stress of freshwater resources deficiency under, how to utilize the resources such as the salt-soda soil of large area, Desertification Soil hair Exhibition agricultural, becomes key subjects.
Cultivate salt-tolerant plant kind by agricultural biotechnologies or utilization have the Halophyte Resources of economic value with Improve salinized soil.Therefore exploitation is to be effectively guaranteed to fill plant by biological approach suitable for the bio-bacterial manure of saline-alkali environment Divide and adapt to salt marsh environment.It is the new direction of recent domestic improvement of salt and alkaline land.
In summary, the existing technology has inconveniences and defects in actual use, so it is necessary to be improved.
The content of the invention
For it is above-mentioned the defects of, it is an object of the invention to provide a kind of salt-soda soil growth-promoting bio-bacterial manure, by that will pass through Liquid fermentation and solid fermentation are made trichoderma asperellum solid spawn, bacillus subtilis solid spawn and bacillus licheniformis and consolidate It is simultaneously pressed 1 by body strain:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；Then by weight addition turfy soil, urine Element, calcium magnesium phosphate, vermiculite, potassium sulfate, concave convex rod, be then stirred 1.5 it is small when, and in 28 DEG C of temperature, the condition of pH 7.8 Under, through be granulated, dry, sieve be made salt-soda soil growth-promoting bio-bacterial manure.The bio-bacterial manure is subjected to the salt-soda soil plantation fluffy growth-promoting of alkali Experiment, fluffy survival rate >=95% of alkali.
To achieve these goals, the present invention provides a kind of salt-soda soil growth-promoting bio-bacterial manure, including
A：Complex micro organism fungicide produces
A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initially PH value 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 165-175rpm；Ferment 24 it is small when, fermentation Liquid bacterium number >=2,000,000,000/ml；
B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains Trichoderma asperellum solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C sterilizing 30min；
C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,33-35 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and lichens gemma Bacillus solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
B：Growth-promoting bio-bacterial manure
By complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, bumps Rod weigh and then be stirred in proportion 1.5-2 it is small when, under conditions of 28-32 DEG C of temperature, pH 7.8-8.2, through being granulated, drying Salt-soda soil growth-promoting bio-bacterial manure is made in dry, screening；
The ratio is weight ratio：5-15 parts of complex micro organism fungicide, 5-20 parts of turfy soil, 15-50 parts of urea, calcium and magnesium 5-25 parts of phosphate fertilizer, 10-30 parts of vermiculite, 5-30 parts of potassium sulfate, 1-5 parts of concave convex rod.
The PDA cultivates liquid and preparation method thereof：Potato cleans peeling, then weighs 200g and be cut into small pieces, and adds boiling rotten；Eight Layer filtered through gauze, adds 15-20g agar；Continue heating stirring to mix until agar dissolves；Glucose is added, is stirred evenly, is cooled down Moisture is supplied again afterwards to 1000 milliliters；110-120 DEG C sterilizes 15-20 minutes, is stored after cooling spare.
The steamed beef soup peptone liquid fermentation medium preparation method is：Beef extract 3g, peptone 10g, NaCl 3g, Water 1L, pH value 7.0-7.2,120-125 DEG C of sterilizing 20-25min.
A：Complex micro organism fungicide produces
A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initially PH value 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 170rpm；Ferment 24 it is small when, zymotic fluid bacterium >=20 hundred million/ml of number；
B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains Trichoderma asperellum solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C sterilizing 30min；
C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 1675rpm； Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and lichens gemma Bacillus solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
B：Growth-promoting bio-bacterial manure
By complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, bumps Rod weigh and then be stirred in proportion 2 it is small when, under conditions of 30 DEG C of temperature, pH 8, through be granulated, dry, sieve be made salt The growth-promoting bio-bacterial manure of alkali ground；
The ratio is weight ratio：8 parts of complex micro organism fungicide, 8 parts of turfy soil, 40 parts of urea, 10 parts of calcium magnesium phosphate, leech 10 parts of stone, 23 parts of potassium sulfate, 1 part of concave convex rod.
A：Complex micro organism fungicide produces
A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initially PH value 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 175rpm；Ferment 24 it is small when, zymotic fluid bacterium >=20 hundred million/ml of number；
B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains Trichoderma asperellum solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C sterilizing 30min；
C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,33 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 170rpm； Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and lichens gemma Bacillus solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
B：Growth-promoting bio-bacterial manure
By complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, bumps Rod weigh and then be stirred in proportion 1.8 it is small when, under conditions of 29 DEG C of temperature, pH 7.9, through being granulated, drying, the system of sieving Obtain salt-soda soil growth-promoting bio-bacterial manure；
The ratio is weight ratio：10 parts of complex micro organism fungicide, 8 parts of turfy soil, 35 parts of urea, 8 parts of calcium magnesium phosphate, leech 12 parts of stone, 25 parts of potassium sulfate, 2 parts of concave convex rod；
C. growth-promoting bio-bacterial manure in salt-soda soil made from step B is subjected to the salt-soda soil plantation fluffy Biological control of alkali, the fluffy survival of alkali Rate is 100%.
A：Complex micro organism fungicide produces
A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initially PH value 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 165rpm；Ferment 24 it is small when, zymotic fluid bacterium >=20 hundred million/ml of number；
B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains Trichoderma asperellum solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C sterilizing 30min；
C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,34 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 135rpm； Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and lichens gemma Bacillus solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
B：Growth-promoting bio-bacterial manure
By complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, bumps Rod weigh and then be stirred in proportion 1.5 it is small when, under conditions of 32 DEG C of temperature, pH 8.2, through being granulated, drying, the system of sieving Obtain salt-soda soil growth-promoting bio-bacterial manure；
The ratio is weight ratio：10 parts of complex micro organism fungicide, 15 parts of turfy soil, 15 parts of urea, 16 parts of calcium magnesium phosphate, 30 parts of vermiculite, 10 parts of potassium sulfate, 4 parts of concave convex rod.
A：Complex micro organism fungicide produces
A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initially PH value 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 175rpm；Ferment 24 it is small when, zymotic fluid bacterium >=20 hundred million/ml of number；
B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains Trichoderma asperellum solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C sterilizing 30min；
C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 185rpm； Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and lichens gemma Bacillus solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
B：Growth-promoting bio-bacterial manure
By complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, bumps Rod weigh and then be stirred in proportion 1.5 it is small when, under conditions of 28 DEG C of temperature, pH 7.8, through being granulated, drying, the system of sieving Obtain salt-soda soil growth-promoting bio-bacterial manure；
The ratio is weight ratio：5 parts of complex micro organism fungicide, 6 parts of turfy soil, 45 parts of urea, 10 parts of calcium magnesium phosphate, leech 10 parts of stone, 20 parts of potassium sulfate, 4 parts of concave convex rod.
Salt-soda soil growth-promoting bio-bacterial manure carries out the salt-soda soil plantation fluffy Biological control of alkali, fluffy survival rate >=95% of alkali.
It is an object of the invention to provide a kind of salt-soda soil growth-promoting bio-bacterial manure, is sent out by that will pass through liquid fermentation and solid Trichoderma asperellum solid spawn, bacillus subtilis solid spawn and bacillus licheniformis solid spawn is made and by it by 1 in ferment: 1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；Then by weight add turfy soil, urea, calcium magnesium phosphate, vermiculite, Potassium sulfate, concave convex rod, be then stirred 1.5 it is small when, and under conditions of 28 DEG C of temperature, pH 7.8, through being granulated, drying, sieving Divide obtained salt-soda soil growth-promoting bio-bacterial manure.By the bio-bacterial manure carry out salt-soda soil plantation the fluffy Biological control of alkali, the fluffy survival rate of alkali >= 95%.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to Limit the present invention.
The present invention provides a kind of salt-soda soil growth-promoting bio-bacterial manure, it comprises the following steps：
A：Complex micro organism fungicide produces
A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initially PH value 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 165-175rpm；Ferment 24 it is small when, fermentation Liquid bacterium number >=2,000,000,000/ml；
B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains Trichoderma asperellum solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C sterilizing 30min；
C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,33-35 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and lichens gemma Bacillus solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
B：Growth-promoting bio-bacterial manure
By complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, bumps Rod weigh and then be stirred in proportion 1.5-2 it is small when, under conditions of 28-32 DEG C of temperature, pH 7.8-8.2, through being granulated, drying Salt-soda soil growth-promoting bio-bacterial manure is made in dry, screening；
The ratio is weight ratio：5-15 parts of complex micro organism fungicide, 5-20 parts of turfy soil, 15-50 parts of urea, calcium and magnesium 5-25 parts of phosphate fertilizer, 10-30 parts of vermiculite, 5-30 parts of potassium sulfate, 1-5 parts of concave convex rod；
Preferably, PDA of the invention culture liquid and preparation method thereof is：Potato cleans peeling, then weigh 200g be cut into it is small Block, adds boiling rotten；Eight layers of filtered through gauze, add 15-20g agar；Continue heating stirring to mix until agar dissolves；Add grape Sugar, stirs evenly, and supplies moisture after cooling again to 1000 milliliters；110-120 DEG C sterilizes 15-20 minutes, is stored after cooling spare.
Preferably, steamed beef soup peptone liquid fermentation medium preparation method of the invention is：Beef extract 3g, peptone 10g, NaCl 3g, water 1L, pH value 7.0-7.2,120-125 DEG C of sterilizing 20-25min；
To obtain the preferred forms of production method, inventor has carried out salt-soda soil plantation alkali using different embodiments Fluffy Biological control the results are shown in Table 1：
Embodiment one：
A：Complex micro organism fungicide produces
A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initially PH value 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 170rpm；Ferment 24 it is small when, zymotic fluid bacterium >=20 hundred million/ml of number；
B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains Trichoderma asperellum solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C sterilizing 30min；
C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 1675rpm； Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and lichens gemma Bacillus solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
B：Growth-promoting bio-bacterial manure
By complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, bumps Rod weigh and then be stirred in proportion 2 it is small when, under conditions of 30 DEG C of temperature, pH 8, through be granulated, dry, sieve be made salt The growth-promoting bio-bacterial manure of alkali ground；
The ratio is weight ratio：8 parts of complex micro organism fungicide, 8 parts of turfy soil, 40 parts of urea, 10 parts of calcium magnesium phosphate, leech 10 parts of stone, 23 parts of potassium sulfate, 1 part of concave convex rod；
C. growth-promoting bio-bacterial manure in salt-soda soil made from step B is subjected to the salt-soda soil plantation fluffy Biological control of alkali, the fluffy survival of alkali Rate is 100%.
Embodiment two：
A：Complex micro organism fungicide produces
A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initially PH value 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 175rpm；Ferment 24 it is small when, zymotic fluid bacterium >=20 hundred million/ml of number；
B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains Trichoderma asperellum solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C sterilizing 30min；
C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,33 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 170rpm； Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and lichens gemma Bacillus solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
B：Growth-promoting bio-bacterial manure
By complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, bumps Rod weigh and then be stirred in proportion 1.8 it is small when, under conditions of 29 DEG C of temperature, pH 7.9, through being granulated, drying, the system of sieving Obtain salt-soda soil growth-promoting bio-bacterial manure；
The ratio is weight ratio：10 parts of complex micro organism fungicide, 8 parts of turfy soil, 35 parts of urea, 8 parts of calcium magnesium phosphate, leech 12 parts of stone, 25 parts of potassium sulfate, 2 parts of concave convex rod；
C. growth-promoting bio-bacterial manure in salt-soda soil made from step B is subjected to the salt-soda soil plantation fluffy Biological control of alkali, the fluffy survival of alkali Rate is 100%.
Embodiment three：
A：Complex micro organism fungicide produces
A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initially PH value 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 165rpm；Ferment 24 it is small when, zymotic fluid bacterium >=20 hundred million/ml of number；
B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains Trichoderma asperellum solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C sterilizing 30min；
C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,34 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 135rpm； Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and lichens gemma Bacillus solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
B：Growth-promoting bio-bacterial manure
By complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, bumps Rod weigh and then be stirred in proportion 1.5 it is small when, under conditions of 32 DEG C of temperature, pH 8.2, through being granulated, drying, the system of sieving Obtain salt-soda soil growth-promoting bio-bacterial manure；
The ratio is weight ratio：10 parts of complex micro organism fungicide, 15 parts of turfy soil, 15 parts of urea, 16 parts of calcium magnesium phosphate, 30 parts of vermiculite, 10 parts of potassium sulfate, 4 parts of concave convex rod；
C. growth-promoting bio-bacterial manure in salt-soda soil made from step B is subjected to the salt-soda soil plantation fluffy Biological control of alkali, the fluffy survival of alkali Rate is 99%.
Example IV：
A：Complex micro organism fungicide produces
A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initially PH value 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 175rpm；Ferment 24 it is small when, zymotic fluid bacterium >=20 hundred million/ml of number；
B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains Trichoderma asperellum solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C sterilizing 30min；
C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid Body fermentation conditions are：Initial pH 6.7-7.2,35 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 185rpm； Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, hair During ferment, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%； The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and lichens gemma Bacillus solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
B：Growth-promoting bio-bacterial manure
By complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, bumps Rod weigh and then be stirred in proportion 1.5 it is small when, under conditions of 28 DEG C of temperature, pH 7.8, through being granulated, drying, the system of sieving Obtain salt-soda soil growth-promoting bio-bacterial manure；
The ratio is weight ratio：5 parts of complex micro organism fungicide, 6 parts of turfy soil, 45 parts of urea, 10 parts of calcium magnesium phosphate, leech 10 parts of stone, 20 parts of potassium sulfate, 4 parts of concave convex rod；
C. growth-promoting bio-bacterial manure in salt-soda soil made from step B is subjected to the salt-soda soil plantation fluffy Biological control of alkali, the fluffy survival of alkali Rate is 98%.
The implementation procedure of other embodiments repeats no more, and table 1 is the various embodiments described above and other section Example is not shown Data.Understand that salt-soda soil growth-promoting bio-bacterial manure prepared by the present invention can be very good to promote salt-soda soil resistance to by above example The survival of alkali plant, survival rate >=95%.
Table 1
In conclusion it is an object of the invention to provide a kind of salt-soda soil growth-promoting bio-bacterial manure, sent out by the way that liquid will be passed through Trichoderma asperellum solid spawn, bacillus subtilis solid spawn and bacillus licheniformis solid spawn is made in ferment and solid fermentation And it is pressed 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；Then by weight addition turfy soil, urea, calcium and magnesium Phosphate fertilizer, vermiculite, potassium sulfate, concave convex rod, be then stirred 1.5 it is small when, and under conditions of 28 DEG C of temperature, pH 7.8, through making Salt-soda soil growth-promoting bio-bacterial manure is made in grain, drying, screening.The bio-bacterial manure is subjected to the salt-soda soil plantation fluffy Biological control of alkali, alkali Fluffy survival rate >=95%.
Certainly, the present invention can also have other various embodiments, ripe in the case of without departing substantially from spirit of the invention and its essence Know those skilled in the art and make various corresponding changes and deformation in accordance with the present invention, but these change and become accordingly Shape should all belong to the protection domain of appended claims of the invention.

Claims (8)

  1. A kind of 1. salt-soda soil growth-promoting bio-bacterial manure, it is characterised in that including
    A：Complex micro organism fungicide produces
    A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initial pH 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 165-175rpm；Ferment 24 it is small when, zymotic fluid bacterium >=20 hundred million/ml of number；
    B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains spine spore Trichoderma solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C go out Bacterium 30min；
    C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid hair Ferment working condition is：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
    D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, ferment Cheng Zhong, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%；It is described Solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
    E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid hair Ferment working condition is：Initial pH 6.7-7.2,33-35 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
    F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, ferment Cheng Zhong, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%；It is described Solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
    G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and bacillus licheniformis Solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
    B：Growth-promoting bio-bacterial manure
    Complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, concave convex rod are pressed Ratio weigh and then be stirred 1.5-2 it is small when, under conditions of 28-32 DEG C of temperature, pH 7.8-8.2, through being granulated, drying, sieving Divide obtained salt-soda soil growth-promoting bio-bacterial manure；
    The ratio is weight ratio：5-15 parts of complex micro organism fungicide, 5-20 parts of turfy soil, 15-50 parts of urea, calcium magnesium phosphate 5- 25 parts, 10-30 parts of vermiculite, 5-30 parts of potassium sulfate, 1-5 parts of concave convex rod.
  2. 2. growth-promoting bio-bacterial manure in salt-soda soil according to claim 1, it is characterised in that the PDA cultivates liquid and preparation method thereof For：Potato cleans peeling, then weighs 200g and be cut into small pieces, and adds boiling rotten；Eight layers of filtered through gauze, add 15-20g agar；Continue Heating stirring mixes until agar dissolves；Glucose is added, stirs evenly, supplies moisture after cooling again to 1000 milliliters；110- 120 DEG C sterilize 15-20 minutes, are stored after cooling spare.
  3. 3. growth-promoting bio-bacterial manure in salt-soda soil according to claim 1, it is characterised in that the steamed beef soup peptone liquid hair Ferment culture medium preparation method is：Beef extract 3g, peptone 10g, NaCl 3g, water 1L, pH value 7.0-7.2,120-125 DEG C of sterilizing 20-25min。
  4. 4. growth-promoting bio-bacterial manure in salt-soda soil according to claim 1, it is characterised in that
    A：Complex micro organism fungicide produces
    A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initial pH 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 170rpm；Ferment 24 it is small when, zymotic fluid bacterium number >= 2000000000/ml；
    B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains spine spore Trichoderma solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C go out Bacterium 30min；
    C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid hair Ferment working condition is：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
    D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, ferment Cheng Zhong, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%；It is described Solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
    E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid hair Ferment working condition is：Initial pH 6.7-7.2,35 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 1675rpm；Fermentation 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
    F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, ferment Cheng Zhong, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%；It is described Solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
    G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and bacillus licheniformis Solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
    B：Growth-promoting bio-bacterial manure
    Complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, concave convex rod are pressed Ratio weigh and then be stirred 2 it is small when, under conditions of 30 DEG C of temperature, pH 8, through be granulated, dry, sieve be made salt-soda soil Growth-promoting bio-bacterial manure；
    The ratio is weight ratio：8 parts of complex micro organism fungicide, 8 parts of turfy soil, 40 parts of urea, 10 parts of calcium magnesium phosphate, vermiculite 10 Part, 23 parts of potassium sulfate, 1 part of concave convex rod.
  5. 5. growth-promoting bio-bacterial manure in salt-soda soil according to claim 1, it is characterised in that
    A：Complex micro organism fungicide produces
    A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initial pH 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 175rpm；Ferment 24 it is small when, zymotic fluid bacterium number >= 2000000000/ml；
    B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains spine spore Trichoderma solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C go out Bacterium 30min；
    C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid hair Ferment working condition is：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
    D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, ferment Cheng Zhong, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%；It is described Solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
    E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid hair Ferment working condition is：Initial pH 6.7-7.2,33 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 170rpm；Fermentation 24 Hour, zymotic fluid bacterium number >=2,000,000,000/ml；
    F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, ferment Cheng Zhong, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%；It is described Solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
    G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and bacillus licheniformis Solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
    B：Growth-promoting bio-bacterial manure
    Complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, concave convex rod are pressed Ratio weigh and then be stirred 1.8 it is small when, under conditions of 29 DEG C of temperature, pH 7.9, through be granulated, dry, sieve be made salt The growth-promoting bio-bacterial manure of alkali ground；
    The ratio is weight ratio：10 parts of complex micro organism fungicide, 8 parts of turfy soil, 35 parts of urea, 8 parts of calcium magnesium phosphate, vermiculite 12 Part, 25 parts of potassium sulfate, 2 parts of concave convex rod；
    C. growth-promoting bio-bacterial manure in salt-soda soil made from step B is subjected to the salt-soda soil plantation fluffy Biological control of alkali, the fluffy survival rate of alkali is 100%.
  6. 6. growth-promoting bio-bacterial manure in salt-soda soil according to claim 1, it is characterised in that
    A：Complex micro organism fungicide produces
    A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initial pH 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 165rpm；Ferment 24 it is small when, zymotic fluid bacterium number >= 2000000000/ml；
    B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains spine spore Trichoderma solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C go out Bacterium 30min；
    C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid hair Ferment working condition is：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
    D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, ferment Cheng Zhong, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%；It is described Solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
    E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid hair Ferment working condition is：Initial pH 6.7-7.2,34 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 135rpm；Fermentation 24 Hour, zymotic fluid bacterium number >=2,000,000,000/ml；
    F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, ferment Cheng Zhong, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%；It is described Solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
    G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and bacillus licheniformis Solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
    B：Growth-promoting bio-bacterial manure
    Complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, concave convex rod are pressed Ratio weigh and then be stirred 1.5 it is small when, under conditions of 32 DEG C of temperature, pH 8.2, through be granulated, dry, sieve be made salt The growth-promoting bio-bacterial manure of alkali ground；
    The ratio is weight ratio：10 parts of complex micro organism fungicide, 15 parts of turfy soil, 15 parts of urea, 16 parts of calcium magnesium phosphate, vermiculite 30 parts, 10 parts of potassium sulfate, 4 parts of concave convex rod.
  7. 7. growth-promoting bio-bacterial manure in salt-soda soil according to claim 1, it is characterised in that
    A：Complex micro organism fungicide produces
    A. trichoderma asperellum is inoculated into PDA nutrient solutions, carried out liquid fermentation；The liquid fermentation working condition is：Initial pH 6.7-7.2,25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 175rpm；Ferment 24 it is small when, zymotic fluid bacterium number >= 2000000000/ml；
    B. trichoderma asperellum zymotic fluid made from step a is inoculated into solid fermentation matrix, ferments 6 days and low temperature drying obtains spine spore Trichoderma solid spawn；Inoculum concentration is volume ratio 7%；The solid fermentation matrix is vinegar groove：Wheat bran mass ratio 5:1,120 DEG C go out Bacterium 30min；
    C. bacillus subtilis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid hair Ferment working condition is：Initial pH 6.7-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 130- 190rpm；Ferment 24 it is small when, zymotic fluid bacterium number >=2,000,000,000/ml；
    D. bacillus subtilis fermentation liquor made from step c is inoculated into solid fermentation matrix, 37 DEG C ferment 5 days, ferment Cheng Zhong, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus subtilis solid spawn；Inoculum concentration is volume ratio 10%；It is described Solid fermentation matrix is vinegar groove：Wheat bran mass ratio 3:1,120 DEG C of sterilizing 30min；
    E. bacillus licheniformis is inoculated into steamed beef soup peptone liquid fermentation medium, carried out liquid fermentation；The liquid hair Ferment working condition is：Initial pH 6.7-7.2,35 DEG C of cultivation temperature, dissolved oxygen throughput scope 40-95%, 185rpm；Fermentation 24 Hour, zymotic fluid bacterium number >=2,000,000,000/ml；
    F. the lichen bacillus ferments liquid made from step e is inoculated into solid fermentation matrix, 35 DEG C ferment 7 days, ferment Cheng Zhong, it is every 18 it is small when stirring once；Low temperature drying obtains bacillus licheniformis solid spawn；Inoculum concentration is volume ratio 10%；It is described Solid fermentation matrix is vinegar groove：Wheat bran mass ratio 4:1,120 DEG C of sterilizing 30min；
    G. by the trichoderma asperellum solid spawn obtained by step b, d, f, bacillus subtilis solid spawn and bacillus licheniformis Solid spawn presses 1:1:1 mixes and thoroughly crushes to obtain complex micro organism fungicide；
    B：Growth-promoting bio-bacterial manure
    Complex micro organism fungicide made from step A and turfy soil, urea, calcium magnesium phosphate, vermiculite, potassium sulfate, concave convex rod are pressed Ratio weigh and then be stirred 1.5 it is small when, under conditions of 28 DEG C of temperature, pH 7.8, through be granulated, dry, sieve be made salt The growth-promoting bio-bacterial manure of alkali ground；
    The ratio is weight ratio：5 parts of complex micro organism fungicide, 6 parts of turfy soil, 45 parts of urea, 10 parts of calcium magnesium phosphate, vermiculite 10 Part, 20 parts of potassium sulfate, 4 parts of concave convex rod.
  8. 8. the salt-soda soil growth-promoting bio-bacterial manure according to claim 1~7 any one carries out the salt-soda soil plantation fluffy growth-promoting of alkali Experiment, fluffy survival rate >=95% of alkali.
CN201711009804.8A 2017-10-25 2017-10-25 A kind of salt-soda soil growth-promoting bio-bacterial manure Pending CN107935672A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109168400A (en) * 2018-11-12 2019-01-11 白城师范学院 The comprehensive processing method of peanut is planted on a kind of salt-soda soil
CN110156511A (en) * 2019-04-12 2019-08-23 浙江省农业科学院 A kind of biological organic fertilizer and preparation method thereof based on mineral humus and vegetables residuum trichoderma fermentation material
CN112457138A (en) * 2020-12-21 2021-03-09 东北农业大学 Microbial preparation for improving soil fertility of saline-alkali soil and preparation method thereof
CN112794774A (en) * 2021-01-13 2021-05-14 南宁市拜欧生物工程有限责任公司 Composite organic liquid biological bacterial fertilizer applied to grape planting and preparation method thereof
CN117343884A (en) * 2023-12-06 2024-01-05 山东君德生态科技有限公司 Preparation method of microbial agent for improving saline-alkali soil

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CN102816005A (en) * 2012-09-10 2012-12-12 临沂中磷生物科技有限公司 Peanut special microbial fertilizer and production method thereof
CN104609995A (en) * 2015-01-22 2015-05-13 河海大学 Plant growth promoting bio-organic fertilizer for saline-alkali land

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Publication number Priority date Publication date Assignee Title
CN102816005A (en) * 2012-09-10 2012-12-12 临沂中磷生物科技有限公司 Peanut special microbial fertilizer and production method thereof
CN104609995A (en) * 2015-01-22 2015-05-13 河海大学 Plant growth promoting bio-organic fertilizer for saline-alkali land

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109168400A (en) * 2018-11-12 2019-01-11 白城师范学院 The comprehensive processing method of peanut is planted on a kind of salt-soda soil
CN110156511A (en) * 2019-04-12 2019-08-23 浙江省农业科学院 A kind of biological organic fertilizer and preparation method thereof based on mineral humus and vegetables residuum trichoderma fermentation material
CN112457138A (en) * 2020-12-21 2021-03-09 东北农业大学 Microbial preparation for improving soil fertility of saline-alkali soil and preparation method thereof
CN112794774A (en) * 2021-01-13 2021-05-14 南宁市拜欧生物工程有限责任公司 Composite organic liquid biological bacterial fertilizer applied to grape planting and preparation method thereof
CN117343884A (en) * 2023-12-06 2024-01-05 山东君德生态科技有限公司 Preparation method of microbial agent for improving saline-alkali soil
CN117343884B (en) * 2023-12-06 2024-02-23 山东君德生态科技有限公司 Preparation method of microbial agent for improving saline-alkali soil

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