CN107493912A - A kind of cultural method of the high tanshinone red sage root - Google Patents
A kind of cultural method of the high tanshinone red sage root Download PDFInfo
- Publication number
- CN107493912A CN107493912A CN201710855066.2A CN201710855066A CN107493912A CN 107493912 A CN107493912 A CN 107493912A CN 201710855066 A CN201710855066 A CN 201710855066A CN 107493912 A CN107493912 A CN 107493912A
- Authority
- CN
- China
- Prior art keywords
- root
- red sage
- seedling
- sage root
- tanshinone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Soil Sciences (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of cultural method of the high tanshinone red sage root.This method comprises the steps：(1) site preparation；(2) transplant：Transplanting pre-treatment is carried out to the seedling obtained after nursery：(a) selection of seedling, (b) cut root processing, (c) secondary airing, the processing of (d) root dipping, afterwards seedlings plugging plantation；(3) nurturing staff；(4) topping defloration；(5) water-fertilizer conditioning；(6) harvest.The method of the present invention can increase red sage root yield, per mu yield is set to be maintained at more than 300kg, the content of tanshinone component is improved simultaneously, the content of tanshinone in red sage root root system is set to reach 0.55% 0.75%, 0.25% standard significantly larger than specified in pharmacopeia, and the obtained content of tanshinone in salvia miltiorrhiza bunge of method of the present invention can be stablized in the range of 0.55% 0.75%.The red sage root of the present invention can ensure raw materials requirement, reduce enterprise's production cost, have good economic benefit and social benefit.
Description
Technical field
The invention belongs to red sage root cultivation field, and in particular to a kind of cultural method of the high tanshinone red sage root.
Background technology
The red sage root (Salvia miltiorrhiza Bge.) is Lamiaceae Salvia category herbaceos perennial, with dry root
It is used as medicine with rhizome, belongs to the large Chinese medicine of tradition.The red sage root has promoting blood circulation, inducing meastruation to relieve menalgia, relieving restlessness that clears away heart-fire, the work(of cool blood to disappear carbuncle
Effect, makes it occupy the status that can not shake in cardiovascular and cerebrovascular diseases field, annual requirement is huge.At present, the whole nation has more than 800
Primary formulation enterprise of family production red sage root medicine series, main formulation have tablet, capsule, dripping pill, soft capsule, pill, freeze-dried powder
12 kinds of formulations such as agent, parenteral solution, drip solution, aerosol, lozenge, oral liquid, granule, are related to individual kind more than 200.
With flourishing for pharmaceutical industries, requirement of the market to red rooted salvia quality, it is overall present using standards of pharmacopoeia as
Basis, the differentiation trend of demand orientation.Pharmacy corporation, especially produce the section of the modern formulation medicines such as parenteral solution, dripping pill
Ji Xing enterprises, lifting is by a relatively large margin made on the basis of pharmacopeia to the quality requirement of salvia miltiorrhiza raw material, particularly in target component
Content, quality stability etc. have formulated more harsh requirement.The tanshinone content detection for being in contrast is not up to standard
It is of common occurrence, far can not meet the needs of manufacturing enterprise, and be stepped up with production cost, enterprise is especially to fat
There is an urgent need to high-quality salvia miltiorrhiza raw material for the manufacturing enterprise that soluble components have higher requirements to reduce production cost；Red sage root plantation is additional
It is worth relatively low, has greatly hit the enthusiasm of planting household, the planting household for replanting other high added value industrial crops rises year by year, long
This in the past, will have a strong impact on the supply of the red sage root, threaten the industrial chain of the red sage root.Therefore, carrying out raising tanshinone in salvia miltiorrhiza bunge class has
Effective component content and the research of the cultivation mode of red sage root yield, the Technical system for building differentiation are necessarily becoming for industry subdivision
Gesture, and the inevitable requirement of industry development.
The content of the invention
Technical problem solved by the invention is to overcome in the prior art that red sage root planting cost is high, added value is relatively low, kind
Plant that obtained tanshinone content detection is not up to standard, yield is not high, far can not meet the defects of the needs of producing pharmacy corporation,
So as to provide a kind of cultural method of the high tanshinone red sage root.The cultural method of the present invention can carry while red sage root yield is increased
The content of high tanshinone component, the content of tanshinone in red sage root root system is set to reach 0.55-0.75%, significantly larger than in pharmacopeia
Defined 0.25% standard.
The present invention is that solve above-mentioned technical problem by the following technical programs：
The invention provides a kind of cultural method of the high tanshinone red sage root, it comprises the steps：
(1) site preparation：
45-50kg composite fertilizers, wherein N in composite fertilizer are applied in selected every mu of arable land ground：P2O5：K2O mass ratio is
(18-22)：(8-12)：(13-15)；Plough afterwards, deep plough 35-42cm；After mid or late Febuary soil thaws, dig, ridging, ridge
Bottom width 85-90cm, ridge face width 35-40cm, the high 28-32cm in ridge, ridge spacing 85-95cm；
(2) transplant：
Transplanting pre-treatment is carried out to the seedling obtained after nursery：(a) selection of seedling：Seedling selection standard：1 main root,
3-5 bar time roots, main root thick 0.4-0.6cm, the secondary thick 0.2-0.35cm of root；(b) root processing is cut：By the red sage root seedling of selection in (a)
Away from excision root system leading portion at red sage root rhizome 9-11cm after airing；(c) secondary airing；(d) root dipping is handled；
Seedlings plugging is planted afterwards, seeding row spacing 20cm × 15cm；
(3) nurturing staff；
(4) topping defloration；
(5) water-fertilizer conditioning：
Treat that relative moisture of the soil is down to 18-22%, continue to be kept for arid 5-8 days, then water, reached to relative moisture of the soil
Stop watering after to 55-65%；
Late July, every mu of cooperation impose potassium dihydrogen phosphate 2.5-3.5kg and composite fertilizer 13-17kg, wherein N in composite fertilizer：
P2O5：K2O mass ratio is 15：15：15；
(6) harvest.
In step (1), those skilled in the art know typically to select soil layer deep (thickness G T.GT.GT 1m), physical features in selection of land
On the sunny side, draining is good, red sage root planting base and nursery base of the soil as high tanshinone in alkalescence, middle fertility,
Generally rufous silt loam, more preferably, the sand grain content of selected rufous silt loam is 55%-65%, percentage
For mass percent.
In step (1), in selection of land, the arable land can have preceding crop, and the preceding crop is preferably wheat, corn
Or Chinese medicine of non-tubers etc..
In step (1), the site preparation known in this field is carried out after the completion of preceding crop harvesting.
In step (1), N in the composite fertilizer：P2O5：K2O mass ratio is preferably 20：10：15.
In step (1), the thickness of the deep ploughing is preferably 40cm.
In step (1), the described those skilled in the art that dig know to turn over to be shallow, preferably shallow to turn over once.
In step (1), the broken such as face of ridging Hou Long faces soil, ridge is directly such as line, and ridge body is full, in turtleback shape.
In step (1), moisture is preferably poured in the ridge face, more preferably covers black degradable mulch, and beaten with machine
Hole, wherein, degradable mulch starts the Biodegradable films of degraded for preferably 100 days or so.
In step (1), the spacing on the ridge is preferably 90cm.
In step (2), the operation and condition of the operation of the nursery and condition for the conventional nursery in this area, it is preferred that
The nursery of the nursery plays flat furrow, set direction North and South direction；It is preferred that nursery selection germination percentage >=70.0%, only
Degree >=60.0%, mass of 1000 kernel >=1.8g, " the Shandong red sage root 1 " salvia seeds of the mature and plump of moisture≤9.00% carry out nursery,
Salvia seeds selected by every mu of sowing 4kg.
Wherein, this area routine operation can carry out vernalization typically before the sowing to seed, preferably use 18-
22mg/L gibberellin processing seed immersion 10-15s.
Wherein, described to sow the sowing being known in the art, the time of the sowing is preferably early July；It is described to broadcast
The mode of kind is preferably drilling.
Wherein, the after planting preferably earthing 0.5-1cm, cover straw pour it is permeable, to prevent direct sunlight.
In step (2), those skilled in the art know to select the seedling of healthy no disease and pests harm in seedling selection.Preferably
Ground, the non-bolting of seedling plant, have and divide root since reed head, have the difference of primary and secondary root, the epiblem of the seedling is scarlet
Color；The nursery of the seedling and the height above sea level difference of planting site are preferably >=400m.
In step (2), the operation and condition of the operation of an airing and condition for the conventional airing in this area, preferably
Ground is that the red sage root seedling is placed in into ventilating and cooling place, and the thickness of an airing is preferably 3-5cm, an airing
Time be preferably 1-2 days, those skilled in the art know that airing is a process for making moisture natural evaporation, it is described once
It is preferable that the Gen Tiao Microsofts of the seedling, roll over continuous after airing.
In step (2), described section of root is section root that those skilled in the art know, is preferably carried out from cleaning cutter,
Described section of root is more preferably preferably tangent along root bar vertical direction tangent and/or beveling.Those skilled in the art know typically
In the sterilization that need to be carried out disinfection before to cutter, preferably sterilized using alcohol disinfecting, the concentration of alcohol used is preferably
60-80%, it is more preferably 70%.Those skilled in the art know that typically beveling should avoid the angle too small cut off and cause dehydration
It is easy to break and then seedling is caused to damage when excessively, transplanting, it is preferred that the beveling scope is 45 ° -90 °.Those skilled in the art
Know can typically there be 0.5-1cm scale error when carrying out described section of root, this error range belongs to this area and is subjected to
Error range.Described section of root is preferably away from excision root system leading portion at red sage root rhizome 10cm.
In step (2), the operation and condition of the operation of the secondary airing and condition for the conventional airing in this area, preferably
Ground is that the red sage root seedling is placed in into ventilating and cooling place, and the time of the secondary airing is preferably 6-48h, is more preferably 12-
36h, more preferably it is further 24h, the thickness of the secondary airing is preferably 3-10cm, is more preferably 3-5cm.It is described secondary
It is preferable that the notching edge shrinkage of the seedling, section water stain-free after airing.
In step (2), operation and condition that the root dipping is handled are operation and the condition of the root dipping processing of this area routine,
Preferably by the mycorhiza agent aqueous solution root dipping 10s of the red sage root seedling in step (c), third time airing is carried out afterwards.The bacterium
The concentration of the root agent aqueous solution be the mycorhiza agent aqueous solution commonly used in the art concentration, preferably 1:(100-1000), v/
V, it is more preferably 1:(200-500), v/v.The mycorhiza agent is commercially available, is mycorhiza agent commonly used in the art, preferably
For purchased from Chinese forest-science academy's forestry research mycorhiza agent (trade name plant strong treasured).It is true that mycorhiza is typically contained in the mycorhiza agent
Bacterium, it is mycorrhizal fungi commonly used in the art, preferably clump branch vesicle bacterium (Vesicalar-Arbuscular).It is described
The operation of third time airing and condition are operation and the condition of the conventional airing in this area, preferably put the red sage root seedling
In ventilating and cooling place.The third time airing is it is preferable that the surface water stain-free of the root of the seedling.
In step (2), the transplanting typically starts in late Febuary next year.
In step (2), operation and condition that the seedlings plugging is planted are operation and the condition of the seedlings plugging plantation of this area routine,
It is preferred that the seedlings plugging plantation is carried out from transplanting machine, the arrangement of seedling "the" shape, overlay film, transplanting, watering are disposable complete
Into.
In step (3), the operation and condition of the operation of the nurturing staff and condition for the conventional nurturing staff in this area,
Preferably every FeSO of 15 days foliage-sprays in May4·7H2The mixed liquor or Luo of O and urea imitate wang aqueous solution, spray two altogether
It is secondary, for strengthening plant leaf blade photosynthesis and accelerating moisture evaporation.
Wherein, the FeSO4·7H2O is FeSO commonly used in the art4·7H2O, the urea are that this area is conventional
The urea used, FeSO in the mixed liquor4·7H2O mass percent is preferably 0.1-0.3%, the quality percentage of urea
Than being preferably 0.1-0.3%, the FeSO4·7H2The dosage of O and urea mixed solution is preferably 30kg/ mus.
Wherein, the Luo effect kings imitate king for Luo commonly used in the art, commercially available, are preferably imitated purchased from Henan Luo
The Luo effect kings of king biotech inc, the mass percent of the Luo effects wang aqueous solution are preferably 0.08-
0.1%, amount of application is generally 30kg/ mus.
In step (3), during the nurturing staff, weeding typically need to be carried out in June, is preferably entered with vegetable garden mini-tiller
Row tillage and weeding, is ploughed deeply.
In step (4), described topping defloration those skilled in the art know its implication, are usually wiped out along spray base portion
Whole spray, the work handled reserved dry branch, basic branch or side shoot, the operation of the topping defloration and condition are this
The operation of the conventional topping defloration in field and condition, are preferably monthly pinched once in the 6-8 months, are preferably entered using hay mover
OK, described pinch preferably removes 48-52% spray, more preferably to remove 50% spray.
In step (5), the operation and condition of the operation of the watering and condition for the conventional watering in this area, preferably
Carried out by the way of sprinkling irrigation.
Relative moisture of the soil is preferably kept in step (5), after mid-August, and in 30%-35%, its purpose is to continue to pierce
Swash root elongation and the increase of root bar number.
In step (5), the purpose of late July top dressing is balance fertilizing, increases root/shoot ratio, coordinates the root system of water stress to mend
Mechanism is repaid, cultivates strengthening root, reduces the quantity of fibrous root.
In step (5), it is preferred that spraying within every 15 days a foliar fertilizer, the operation for spraying foliar fertilizer and bar in September part
Part is the conventional operation and the condition that spray foliar fertilizer in this area, and preferably September part sprays twice altogether.
Wherein, described foliar fertilizer is foliar fertilizer commonly used in the art, preferably FeSO4·7H2O and KH2PO4
Mixture or Luo effect wang aqueous solution.
Wherein, the FeSO4·7H2O and KH2PO4Mixture in preferably FeSO4·7H2The O aqueous solution and
KH2PO4The aqueous solution mixture.
Wherein, the FeSO4·7H2The O aqueous solution is FeSO commonly used in the art4·7H2The O aqueous solution, preferably
Ground, the FeSO4·7H2FeSO in the O aqueous solution4·7H2O mass percents are 0.1-0.3%, are more preferably 0.2%.
Wherein, the KH2PO4The aqueous solution be KH commonly used in the art2PO4The aqueous solution, it is preferred that the KH2PO4
KH in the aqueous solution2PO4Mass percent be 0.2-0.4%, be more preferably 0.3%.
Wherein, the FeSO4·7H2O and KH2PO4The dosage of mixture be the conventional dosage in this area, preferably
30kg/ mus.
Wherein, the Luo effect kings imitate king for Luo commonly used in the art, commercially available, are preferably imitated purchased from Henan Luo
The Luo effect kings of king biotech inc, the mass percent of the Luo effects wang aqueous solution are preferably 0.18-
0.2%, amount of application is generally 45kg/ mus.
In step (5), the purpose that September sprays foliar fertilizer is enhancing red sage root photosynthesis, ensures that blade plumpness is dark green, ensures
There are enough nutrition supplies root.
In step (6), the harvesting preferably progress at the beginning of 11 months, the mode of the harvesting preferably mechanical harvesting.
Preferably first aerial part is cut off before the harvesting with hay mover.
It on the basis of common sense in the field is met, above-mentioned each optimum condition, can be combined, it is each preferably real to produce the present invention
Apply example.
Agents useful for same and raw material of the present invention are commercially available.
The positive effect of the present invention is：
The cultural method of the present invention, the theory of " quality comes from design " is fused in red sage root cultivation production, to Salvia miltiorrhiza
The regulation and control that the related key node selection of land of material cultivation, site preparation, culturing and transplanting seedlings, water-fertilizer conditioning, field management etc. are become more meticulous, with
The Technical system for the differentiation that whole quality management structure purposes is oriented to.
The cultural method of the present invention is easy to medicinal herb grower to grasp, and is easy to promote, can increase red sage root yield, be maintained at per mu yield
More than 300kg, while the content of tanshinone component is improved, the content of tanshinone in red sage root root system is reached 0.55%-
0.75%, 0.25% standard significantly larger than specified in pharmacopeia, and the red sage root in the obtained red sage root of cultural method of the present invention
Ketone content can be stablized in the range of 0.55%-0.75%.
The red sage root quality that the method for the present invention plants to obtain is high, can ensure that medicine and cosmetics, the raw material of health products need
Ask, reduce the extraction cost of manufacturing enterprise, contributed for big health industry, improve the income of planting household again, lift planting household
Enthusiasm, the in turn supply for the high-quality red sage root provide safeguard, and have good economic benefit and social benefit.
Brief description of the drawings
Fig. 1 is the high special seed and seedling traits photo of tanshinone content in the embodiment of the present invention 1.
Fig. 2 is the character photo for the red rooted salvia that high tanshinone content is finally given in the embodiment of the present invention 1.
Embodiment
The present invention is further illustrated below by the mode of embodiment, but does not therefore limit the present invention to described reality
Apply among a scope.The experimental method of unreceipted actual conditions in the following example, conventionally and condition, or according to business
Product specification selects.
Tanshinone content in example 1 below and comparative example 1-15 is surveyed according to high performance liquid chromatography (general rule 0512)
It is fixed.
1st, chromatographic condition and system suitability
Using octadecylsilane chemically bonded silica as filler；Using acetonitrile as mobile phase A, using 0.02% phosphoric acid solution as flowing
Phase B, the regulation according to the form below carry out gradient elution；Column temperature is 20 DEG C；Detection wavelength is 270nm.Number of theoretical plate presses Tanshinone I I
A peaks, which calculate, should be not less than 60 000.
2nd, the preparation of reference substance solution
Take Tanshinone I IAAppropriate reference substance, it is accurately weighed, put in brown measuring bottle, add methanol that every l ml are made and contain 20 μ g's
Solution, produce.
3rd, the preparation of need testing solution
Sample powder (crossing No. three sieves) about 0.3g is taken, it is accurately weighed, to put in conical flask with cover, precision adds methanol 50ml,
Close plug, weighed weight, (power 140W, frequency 42kHz) 30min is ultrasonically treated, is let cool, then weighed weight, supplied and subtracted with methanol
The weight of mistake, shakes up, and filtration, takes subsequent filtrate, produces.
4th, determination method
It is accurate respectively to draw reference substance solution and each 10 μ l of need testing solution, inject liquid chromatograph.With Tanshinone I IAIt is right
It is reference according to product, using its corresponding peak as S peaks, Cryptotanshinone, the relative retention time of Tanshinone I is calculated, when it retains relatively
Between should be within the scope of ± the 5% of setting.Relative retention time and correction are because see the table below：
| Composition (peak) to be measured | Relative retention time | Correction factor |
| Cryptotanshinone | 0.75 | 1.18 |
| Tanshinone | 0.79 | 1.31 |
| Cryptotanshinone IIA | 1.00 | 1.00 |
5th, criterion
With Tanshinone I IAPeak area be control, be multiplied by correction factor respectively, calculate Cryptotanshinone, Tanshinone I, the red sage root
Ketone IIAContent.Tanshinone is calculated by dry product, I containing Tanshinone IA(C19H18O3), Cryptotanshinone (C19H20O3) and Tanshinone I
(C18H12O3) total amount must not be less than 0.25%.
Embodiment 1
(1) site preparation
Soil layer deep (＞ 1m), physical features is selected to face south, draining is good, in the rufous flour sand earth of alkalescence, medium fertility
The red sage root planting base and nursery base of native (sand grain content 55%-65%) as high tanshinone.Select wheat, corn and
The preceding crop as planting base and nursery base such as Chinese medicine of non-tubers.
Base should carry out site preparation after the completion of preceding crop harvesting, and deep plough 40cm.Every mu is applied (N：P2O5：K2O=20：10：
15) base fertilizer of the composite fertilizer 50kg as the red sage root planting base of high tanshinone, nitrogen phosphoris and potassium fertilizer ratio is more crucial, because high
Nitrogen level can promote red sage root aerial growth, reduce root/shoot ratio, and facility is provided for later stage water-fertilizer conditioning, reduce liquid manure and adjust
The time required to control, while and can takes into account underground part growth.
It is shallow to turn over once after mid or late Febuary soil thaws.Play high ridge (stretching, extension for being easy to red sage root root system), ridge bottom width 85-
90cm, ridge face width 35-40cm, the high 30cm in ridge, ridge spacing 90cm.It is required that the broken such as face of ridge face soil, directly such as line, ridge body is full, is on ridge
Turtleback shape.Moisture is poured in ridge face in advance, and covering black degradable mulch (improves ground temperature, promotes the quick slow seedling of the red sage root and growth, together
When can accelerate the evaporation rate of soil moisture, reduce the water-fertilizer conditioning stand-by period).
(2) transplant
Nursery first, nursery play flat furrow, and direction preferably selects North and South direction (to be easy to summer ventilation, autumn and winter to receive more
More sunlight).Mid-June to mid-July, select germination percentage >=70.0%, cleanliness >=60.0%, mass of 1000 kernel >=1.8g, moisture
" the Shandong red sage root 1 " salvia seeds of≤9.00% mature and plump carry out nursery, and every mu is used before with 4kg salvia seeds, sowing
20mg/L gibberellin processing seed immersion 10-15s, drilling.Earthing 0.5-1cm.Cover straw and pour permeable, prevent that sunlight is straight
Penetrate.
Transplanting pre-treatment is carried out to the seedling selected after nursery：Healthy no disease and pests harm is selected, the non-bolting of plant, is had from reed
Head starts a point root, there is the difference of primary and secondary root, 1 main root, 3-5 bar time roots, main root thick 0.4-0.6cm, the secondary thick 0.2-0.35cm of root；Kind
The epiblem of seedling be cherry seedling as propagating materials, see Fig. 1.Cut root processing：Red sage root seedling airing is in ventilating and cooling place
24h, thickness 3-5cm, to Gen Tiao Microsofts, folding is constantly, with the sterilized cleaning cutter of 70% alcohol disinfecting away from red sage root rhizome
At 10cm root system leading portion is cut off along root bar vertical direction；The airing of seedling：The red sage root seedling airing after root is cut in ventilating and cooling place
24h, to notching edge shrinkage, section water stain-free, thickness 3-5cm.Root dipping processing：With the mycorhiza agent rich in clump branch vesicle bacterium
(being purchased from Chinese forest-science academy's forestry research institute, plant strong treasured) (1:200, v/v) aqueous solution root dipping 10s, pull out and spread out in ventilation cool place
Place, dries in the air to surface water stain-free, is bundled into standby.
Late Febuary starts to transplant, large-ridge double-row, is planted using red sage root transplanter, "the" shape arrangement, and seeding row spacing 20cm ×
(air line distance, distance too conference cause root system excessively sturdy to 15cm, influence the content of tanshinone component, distance is too small can shadow
Ringing the elongation of root system causes the root system short and thick), ridge spacing 90cm.
(3) nurturing staff
May, per the 15d foliage-sprays FeSO of one time 0.2%4.7H2O and aqueous solution of urea, 2 (enhancing red sage root light are sprayed altogether
Cooperation is used, and ensures that blade plumpness is dark green, accelerates overground part growth, and nutrition is accumulated for later stage root growth, accelerates overground part growth,
Accelerate the evaporation of soil moisture by transpiration pull).
June with vegetable garden mini-tiller tillage and weeding, ploughs deeply and (chesson, accelerates shallow soil layer moisture evaporation, promote root system to depth
Place's growth) nurturing staff is most important, and without nurturing staff, water-fertilizer conditioning can cause substantial amounts of red sage root plant wilting, dehydration, very
To death.
(4) topping defloration
The 6-8 month reds sage root initially enter full-bloom stage, and root growth is slow, monthly with the topping of weeding machine once, remove 50%
(the 6-8 months, the red sage root enter full-bloom stage to the spray of left and right, and substantial amounts of nutrition starts to supply reproductive growth, and the accumulation of tanshinone runs through
Whole reproductive stage, 5-9 is monthly in state is stepped up, and is begun to decline after October.Topping defloration, which can promote to reduce, gives birth to
The nutrient consumption of reproductive growth, promote the growth of underground root system).
(5) water-fertilizer conditioning
Keep relative moisture of the soil to gradually reduce, treat that relative moisture of the soil is down to 20% or so, continue to keep arid one week
Left and right, then watered by the way of sprinkling irrigation, relative moisture of the soil reaches 60% or so, stops watering and (responds soil according to root system
The change of earth humidity produces the principle of corresponding compensation mechanism, and antecedent moisture is suitable, promotes main root growth, with water stress
Aggravation, red sage root root system constantly extend, and in favor of absorbing the moisture of deep subsoil, while can germinate time root, root system continues after rehydration
Elongation) such as have ready conditions, relative moisture of the soil is kept after mid-August in 30%-35%, continues to stimulate root elongation and root bar number
Increase.
Late July, every mu of cooperation impose potassium dihydrogen phosphate 3kg and (N：P2O5：K2O=15：15：15) composite fertilizer 15kg
(balance fertilizing, increase root/shoot ratio.Coordinate the root system compensation mechanism of water stress, cultivate strengthening root, reduce the quantity of fibrous root).
September, per the 15d foliage-sprays FeSO of one time 0.2%4.7H2O and 0.3% KH2PO4, 2 (enhancing reds sage root are sprayed altogether
Photosynthesis, ensure that blade plumpness is dark green).
(6) harvest
Aerial part, mechanical harvesting are cut off at the beginning of 11 months in advance.Fig. 2 is the red rooted salvia for finally giving high tanshinone content
Character photo.
Comparative example 1
Implantation methods of the present invention are not used, are planted using commonsense method.Soil of the common cultivation method to plantation soil
Earth type does not select substantially, and the cultivation of seed seedling selection is random stronger without corresponding standard, red sage root seedling without
Classification is handled, and does not select specific seedling, usually " mashed up " plant of the seedling of different size, and planting patterns is level planting, the plantation of single ridge
Deng, plantation before do not cut root operation, handled without mycorhiza, field management does not typically do water-fertilizer conditioning or only imposes fertilizer, no
Overlay film or seldom overlay film, do not pinch.Whole process mechanization participation is less, and cost is higher, the tanshinone of seedling and output medicinal material
Content is extremely unstable, unbalanced, and yield is relatively low and is difficult to secure.Table 1 be different cultural methods to red sage root root system, yield,
The influence of composition.
1 different cultural methods of table are to red sage root root system, the influence of yield, composition
| Embodiment | Cultivation mode | Root bar number | Fresh yield (g/ strains) | Tanshinone content (%) |
| Embodiment 1 | The method of the present invention | 20-35 | 160-235 | 0.55-0.75 |
| Comparative example 1 | Commonsense method | 8-28 | 80-200 | 0.20-0.60 |
Comparative example 2-4
In comparative example 2-4 it is selected arable land be respectively sand, clay and canescence sandy loam, other operation and condition with reality
It is identical to apply example 1.Table 2 is influence of the Different Soil to red sage root character, yield, content in embodiment 1 and comparative example 2-4.
Influence of the Different Soil to red sage root character, yield, content in the embodiment 1 of table 2 and comparative example 2-4
Comparative example 5-6
Comparative example 5 and 6 selects N respectively in the site preparation stage：P2O5：K2O mass ratioes are 15：15：15 and urea be composite fertilizer,
Other operations and condition are same as Example 1.Table 3 is different nitrogen phosphoris and potassium fertilizer ratios in embodiment 1 and comparative example 5 and 6 to the red sage root
The influence of character, yield, content.
Influence of 3 site preparation stage of the table difference nitrogen phosphoris and potassium fertilizer ratio to red sage root character, yield, content
| Embodiment | N：P2O5：K2O | Root bar number | Fresh yield (g/ strains) | Tanshinone content (%) |
| Embodiment 1 | 20：10：15 | 20-35 | 160-235 | 0.55-0.75 |
| Comparative example 5 | 15：15：15 | 12-21 | 120-220 | 0.40-0.65 |
| Comparative example 6 | Urea | 5-20 | 80-145 | 0.30-0.55 |
Comparative example 7
The seedling without selection is used in comparative example 7, is the kind without classification processing, " mashed up " plant of different size
Seedling, other operations and condition are same as Example 1.Table 4 is red sage root output condition in embodiment 1 and comparative example 7.
Red sage root output condition in the embodiment 1 of table 4 and comparative example 7
| Embodiment | Method for culturing seedlings | Root bar number | Fresh yield (g/ strains) | Tanshinone content (%) |
| Embodiment 1 | The seedling of selection | 20-35 | 160-235 | 0.55-0.75 |
| Comparative example 7 | Do not select seedling | 5-25 | 80-230 | 0.35-0.55 |
Comparative example 8-11
Root is handled without root dipping in the transplanting stage in comparative example 8-10, cut root processing do not subtract respectively root, tiltedly
Cut, straight snips, remaining operation and condition are same as Example 1, in comparative example 11 in the transplanting stage to root with clump branch vesicle bacterium
Reason, do not do and cut root processing, remaining operation and condition are same as Example 5.Table 5 is in embodiment 1 and comparative example 8-11 at root system
Manage the influence to red sage root root system and composition.
Root system handles the influence to red sage root root system and composition in the embodiment 1 of table 5 and comparative example 8-11
| Embodiment | Processing | Root bar number | Root bar distribution consistency degree | Tanshinone content (%) |
| Embodiment 1 | Straight snips clump branch vesicle bacterium is handled | 20-35 | Uniformly | 0.55-0.75 |
| Comparative example 8 | Root is not cut not handle | 5-25 | It is more uniform | 0.30-0.60 |
| Comparative example 9 | Tiltedly cut and do not handle | 12-30 | It is uneven | 0.40-0.65 |
| Comparative example 10 | Straight snips is not handled | 18-28 | Uniformly | 0.45-0.65 |
| Comparative example 11 | The bacterium processing of clump branch vesicle | 15-25 | Uniformly | 0.35-0.60 |
Comparative example 12
It is same as Example 1 without nurturing staff, remaining operation and condition in comparative example 12.Table 6 is embodiment 1 and right
Influence of the nurturing staff to red sage root survival rate in ratio 12.
Influence of the nurturing staff to red sage root survival rate in the embodiment 1 of table 6 and comparative example 12
| Embodiment | Processing | Survival rate (%) |
| Embodiment 1 | Nurturing staff | 85-100 |
| Comparative example 12 | Not nurturing staff | 55-70 |
Comparative example 13
It is same as Example 1 without topping, remaining operation and condition in comparative example 13.Table 7 is embodiment 1 and comparative example
Influence of the topping to red sage root root system and composition in 13.
Influence of the topping to red sage root root system and composition in the embodiment 1 of table 7 and comparative example 13
| Embodiment | Processing | Fresh yield (g/ strains) | Tanshinone content (%) |
| Embodiment 1 | Topping 50% | 160-235 | 0.55-0.75 |
| Comparative example 13 | Do not pinch | 120-175 | 0.45-0.65 |
Comparative example 14
It is same as Example 1 without water-fertilizer conditioning, remaining operation and condition in comparative example 14.Table 8 is embodiment 1 and right
Influence of the water-fertilizer conditioning to red sage root root system and composition in ratio 14.
Influence of the water-fertilizer conditioning to red sage root root system and composition in the embodiment 1 of table 8 and comparative example 14
| Embodiment | Regulation measure | Root bar number | Fresh yield (g/ strains) | Tanshinone content (%) |
| Embodiment 1 | Water-fertilizer conditioning | 20-35 | 160-235 | 0.55-0.75 |
| Comparative example 14 | Do not regulate and control | 15-25 | 150-210 | 0.35-0.50 |
Comparative example 15
Not top dressing in comparative example 15, remaining operation and condition are same as Example 1.Table 9 is in embodiment 1 and comparative example 15
Influence of the top dressing to red sage root root system and composition yield.
Influence of the top dressing to red sage root root system and composition yield in the embodiment 1 of table 9 and comparative example 15
| Embodiment | Top dressing | Root bar number | Fibrous root ratio | Fresh yield (g/ strains) | Tanshinone content (%) |
| Embodiment 1 | Top dressing | 20-35 | 5%-8% | 160-235 | 0.55-0.75 |
| Comparative example 15 | Not top dressing | 15-30 | 18%-30% | 120-180 | 0.40-0.65 |
Although the embodiment of the present invention is the foregoing described, it will be appreciated by those of skill in the art that this is only
For example, protection scope of the present invention is to be defined by the appended claims.Those skilled in the art without departing substantially from
On the premise of the principle and essence of the present invention, various changes or modifications can be made to these embodiments, but these changes and
Modification each falls within protection scope of the present invention.
Claims (10)
1. a kind of cultural method of the high tanshinone red sage root, it is characterised in that it comprises the steps：
(1) site preparation：
45-50kg composite fertilizers, wherein N in composite fertilizer are applied in selected every mu of arable land ground：P2O5：K2O mass ratio is (18-
22)：(8-12)：(13-15)；Plough afterwards, deep plough 35-42cm；After mid or late Febuary soil thaws, dig, ridging, ridge bottom width
85-90cm, ridge face width 35-40cm, the high 28-32cm in ridge, ridge spacing 85-95cm；
(2) transplant：
Transplanting pre-treatment is carried out to the seedling obtained after nursery：(a) selection of seedling：Seedling selection standard：1 main root, 3-5 bars
Secondary root, main root thick 0.4-0.6cm, the secondary thick 0.2-0.35cm of root；(b) root processing is cut：The red sage root seedling of selection in (a) is once spread out
Away from excision root system leading portion at red sage root rhizome 9-11cm after drying in the air；(c) secondary airing；(d) root dipping is handled；
Seedlings plugging is planted afterwards, seeding row spacing 20cm × 15cm；
(3) nurturing staff；
(4) topping defloration；
(5) water-fertilizer conditioning：
Treat that relative moisture of the soil is down to 18-22%, continue to be kept for arid 5-8 days, then water, reach to relative moisture of the soil
Stop watering after 55-65%；
Late July, every mu of cooperation impose potassium dihydrogen phosphate 2.5-3.5kg and composite fertilizer 13-17kg, wherein N in composite fertilizer：P2O5：
K2O mass ratio is 15：15：15；
(6) harvest.
2. the method as described in claim 1, it is characterised in that in step (1), the arable land is rufous silt loam；Compared with
Goodly, the sand grain content of selected rufous silt loam is 55%-65%, and percentage is mass percent；
In step (1), in selection of land, there is preceding crop in the arable land；It is preferred that the preceding crop be wheat, corn or
The Chinese medicine of non-tubers；
In step (1), N in the composite fertilizer：P2O5：K2O mass ratio is 20：10：15；
In step (1), the thickness of the deep ploughing is 40cm；
In step (1), the ridge face covers black degradable mulch, and is punched with machine；
In step (1), the spacing on the ridge is 90cm.
3. the method as described in claim 1, it is characterised in that in step (2), the nursery selects germination percentage >=70.0%,
Cleanliness >=60.0%, mass of 1000 kernel >=1.8g, the salvia seeds of moisture≤9.00% carry out nursery, the red sage root selected by every mu of sowing 4kg
Seed；
In step (2), the epiblem of the seedling is cerise, the nursery of the seedling and the height above sea level of planting site it is poor >=
400m；
In step (2), an airing is that the red sage root seedling is placed in into ventilating and cooling place, and the thickness of an airing is
3-5cm, the time of an airing is 1-2 days；
In step (2), described section of root is carried out with cleaning cutter, described section of root for along root bar vertical direction tangent and/or beveling, compared with
It is tangent goodly；Described section of root is away from excision root system leading portion at red sage root rhizome 10cm；It is preferred that cutter is used before use
Alcohol disinfecting is sterilized, and the concentration of alcohol used is 60-80%, is more preferably 70%；The angular range of the beveling is preferably
45°-90°；
In step (2), the secondary airing is that the red sage root seedling is placed in into ventilating and cooling place, and the time of the secondary airing is
6-48h, preferably 12-36h, it is more preferably 24h, the thickness of the secondary airing is 3-10cm, preferably 3-5cm；
In step (2), root dipping processing be by the red sage root seedling mycorhiza agent aqueous solution root dipping 10s in step (c), it is laggard
Row third time airing；The concentration of the mycorhiza agent aqueous solution is 1:(100-1000), v/v, preferably 1:(200-500), v/
v；The third time airing is that the red sage root seedling is placed in into ventilating and cooling place；
In step (2), the transplanting starts in late Febuary next year；
In step (2), the seedlings plugging plantation is carried out from transplanting machine.
4. method as claimed in claim 3, it is characterised in that vernalization is carried out to seed before the sowing, using 18-
22mg/L gibberellin processing seed immersion 10-15s；The time of the sowing is early July；The mode of the sowing is bar
Broadcast；The after planting earthing 0.5-1cm, waters after covering straw.
5. the method as described in claim 1, it is characterised in that in step (3), the nurturing staff is every 15 days leaves in May
Face sprays a FeSO4·7H2The mixed liquor or Luo of O and urea imitate wang aqueous solution, spray altogether twice；
In step (3), during the nurturing staff, tillage and weeding is carried out using vegetable garden mini-tiller in June, ploughed deeply.
6. method as claimed in claim 5, it is characterised in that FeSO in the mixed liquor4·7H2O mass percent is
0.1-0.3%, the mass percent of urea is 0.1-0.3%, the FeSO4·7H2The dosage of O and urea mixed solution is 30kg/
Mu；
The mass percent of the Luo effects wang aqueous solution is 0.08-0.1%, and amount of application is 30kg/ mus.
7. the method as described in claim 1, it is characterised in that in step (4), the topping defloration is monthly carried out in the 6-8 months
Once, the topping defloration is carried out using hay mover, and described pinch removes 48-52% spray, preferably removes 50%
Spray.
8. the method as described in claim 1, it is characterised in that in step (5), the watering is carried out by the way of sprinkling irrigation；
Relative moisture of the soil is kept in step (5), after mid-August in 30%-35%；
In step (5), spray a foliar fertilizer within every 15 days in September part, spray altogether twice.
9. method as claimed in claim 8, it is characterised in that described foliar fertilizer is FeSO4·7H2O and KH2PO4Mixing
Thing or Luo effect wang aqueous solutions；
The FeSO4·7H2O and KH2PO4Mixture be FeSO4·7H2The O aqueous solution and KH2PO4The aqueous solution mixing
Thing；
The FeSO4·7H2FeSO in the O aqueous solution4·7H2O mass percent is 0.1-0.3%, preferably 0.2%；
The KH2PO4KH in the aqueous solution2PO4Mass percent be 0.2-0.4%, preferably 0.3%；
The FeSO4·7H2O and KH2PO4The dosage of mixture be 30kg/ mus；
The mass percent of the Luo effects wang aqueous solution is 0.18-0.2%, and amount of application is 30kg/ mus.
10. the method as described in claim 1, it is characterised in that in step (6), harvesting progress at the beginning of 11 months is described to adopt
Receive and carried out using machinery, aerial part is cut off with hay mover before the harvesting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710855066.2A CN107493912A (en) | 2017-09-20 | 2017-09-20 | A kind of cultural method of the high tanshinone red sage root |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710855066.2A CN107493912A (en) | 2017-09-20 | 2017-09-20 | A kind of cultural method of the high tanshinone red sage root |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107493912A true CN107493912A (en) | 2017-12-22 |
Family
ID=60697767
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710855066.2A Pending CN107493912A (en) | 2017-09-20 | 2017-09-20 | A kind of cultural method of the high tanshinone red sage root |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107493912A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006291A (en) * | 2018-08-03 | 2018-12-18 | 贵州景诚制药有限公司 | A kind of method of red rooted salvia Guizhou introducing and planting |
| CN109924090A (en) * | 2019-04-23 | 2019-06-25 | 河南省文医堂药业有限公司 | A kind of Radix Salviae Miltiorrhizae implantation methods improving tanshinone content |
| CN113615539A (en) * | 2020-05-07 | 2021-11-09 | 蔡巨才 | Salvia miltiorrhiza cultivation medium and cultivation method |
| CN114902922A (en) * | 2022-05-06 | 2022-08-16 | 四川省天府神龙中药饮片有限公司 | Cultivation method for increasing tanshinone content in salvia miltiorrhiza |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1466866A (en) * | 2002-07-11 | 2004-01-14 | 莱芜市丰盛丹参研究开发中心 | Method for planting white flower red sage root |
| CN101946609A (en) * | 2010-08-16 | 2011-01-19 | 山东省农业科学院农产品研究所 | Seedling cultivation and interplanting method for corn and root of red-rooted salvia |
| CN106305068A (en) * | 2016-08-19 | 2017-01-11 | 上海市药材有限公司 | Seedling growing method for seedlings special for high-tanshinone-content salviae miltiorrhizae |
| CN106797776A (en) * | 2016-12-13 | 2017-06-06 | 韦克康 | Salvia seeds method for culturing seedlings |
| CN107172990A (en) * | 2017-05-04 | 2017-09-19 | 陕西师范大学 | A kind of red sage root crop field propagation method |
-
2017
- 2017-09-20 CN CN201710855066.2A patent/CN107493912A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1466866A (en) * | 2002-07-11 | 2004-01-14 | 莱芜市丰盛丹参研究开发中心 | Method for planting white flower red sage root |
| CN101946609A (en) * | 2010-08-16 | 2011-01-19 | 山东省农业科学院农产品研究所 | Seedling cultivation and interplanting method for corn and root of red-rooted salvia |
| CN106305068A (en) * | 2016-08-19 | 2017-01-11 | 上海市药材有限公司 | Seedling growing method for seedlings special for high-tanshinone-content salviae miltiorrhizae |
| CN106797776A (en) * | 2016-12-13 | 2017-06-06 | 韦克康 | Salvia seeds method for culturing seedlings |
| CN107172990A (en) * | 2017-05-04 | 2017-09-19 | 陕西师范大学 | A kind of red sage root crop field propagation method |
Non-Patent Citations (1)
| Title |
|---|
| 蔺海明，邱黛玉: "《当归标准化生产技术》", 31 March 2010, 金盾出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006291A (en) * | 2018-08-03 | 2018-12-18 | 贵州景诚制药有限公司 | A kind of method of red rooted salvia Guizhou introducing and planting |
| CN109924090A (en) * | 2019-04-23 | 2019-06-25 | 河南省文医堂药业有限公司 | A kind of Radix Salviae Miltiorrhizae implantation methods improving tanshinone content |
| CN113615539A (en) * | 2020-05-07 | 2021-11-09 | 蔡巨才 | Salvia miltiorrhiza cultivation medium and cultivation method |
| CN114902922A (en) * | 2022-05-06 | 2022-08-16 | 四川省天府神龙中药饮片有限公司 | Cultivation method for increasing tanshinone content in salvia miltiorrhiza |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107750874A (en) | A kind of beet increases sugared high yield green planting method | |
| CN107493912A (en) | A kind of cultural method of the high tanshinone red sage root | |
| CN108770617A (en) | Mixed seeding Chinese milk vetch that a kind of double rice cropping system is no-tillage and the method that Radish turns over returning to the field culture fertility | |
| CN102792848A (en) | Method for efficiently producing leaves of tea fertilizer No.1 | |
| CN106134685A (en) | A kind of implantation methods of the Rhizoma Pinelliae | |
| CN109105186B (en) | Cultivation method for strong seedlings with tillers of soil machine transplanting bowl body easy to leak water | |
| CN103843575B (en) | A kind of Radix Angelicae Sinensis seedling-cultivating method | |
| CN107750870A (en) | Improve the implantation methods of wheat quality | |
| CN106171798A (en) | A kind of Picea Mongolica land for growing field crops, Arid Desert Area seed seedling-raising method | |
| CN109644805A (en) | A kind of method of Radix Salviae Miltiorrhizae root segment plantation | |
| CN103563627A (en) | Plastic film mulching ridge-type salviae miltiorrhizae cultivating method | |
| CN106305068A (en) | Seedling growing method for seedlings special for high-tanshinone-content salviae miltiorrhizae | |
| CN105660156A (en) | Mengdan No.1 salvia miltiorrhiza bge.cultivation technology | |
| CN106508347A (en) | Method for cultivation of angelica sinensis | |
| CN113951033A (en) | Method for promoting high yield of durian in warm and humid environment | |
| CN104170624B (en) | A kind of honeysuckle cultivation method | |
| CN110476739B (en) | Method suitable for planting capsicum in Hainan province | |
| CN107711021A (en) | A kind of tomato high-yield planting method | |
| CN110800563A (en) | Planting method of gentiana macrophylla | |
| CN107466660A (en) | The cultural method to feed intake with the red sage root | |
| CN104838858A (en) | Rape stubble soil ploughing-free direct transplanting rice cultivation method | |
| CN115443867A (en) | Bletilla striata wild-imitating planting method suitable for karst regions | |
| CN107711370A (en) | A kind of tuniclike psammosilene root original ecology cultivating technology | |
| CN104186154A (en) | Cultivation method for qualified scutellaria baicalensis planted in north and transplanted to south | |
| CN109566322B (en) | High-yield cultivation method for interplanting long and short fruit branches and island cotton |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171222 |
|
| RJ01 | Rejection of invention patent application after publication |