CN106389180A - 一种银杏原花青素-多糖混合提取物及其制备方法和应用 - Google Patents
一种银杏原花青素-多糖混合提取物及其制备方法和应用 Download PDFInfo
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- CN106389180A CN106389180A CN201510454258.3A CN201510454258A CN106389180A CN 106389180 A CN106389180 A CN 106389180A CN 201510454258 A CN201510454258 A CN 201510454258A CN 106389180 A CN106389180 A CN 106389180A
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- extract
- ginkgo
- opc
- polysaccharide
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- 239000000284 extract Substances 0.000 title claims abstract description 117
- 235000008100 Ginkgo biloba Nutrition 0.000 title claims abstract description 94
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 92
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 92
- 235000011201 Ginkgo Nutrition 0.000 title claims abstract description 62
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 241000218628 Ginkgo Species 0.000 title claims abstract 14
- 238000002156 mixing Methods 0.000 title abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 151
- 150000004676 glycans Chemical class 0.000 claims abstract description 73
- 238000001556 precipitation Methods 0.000 claims abstract description 63
- 238000000605 extraction Methods 0.000 claims abstract description 47
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000004202 carbamide Substances 0.000 claims abstract description 24
- 239000000243 solution Substances 0.000 claims abstract description 19
- 239000007864 aqueous solution Substances 0.000 claims abstract description 3
- 244000194101 Ginkgo biloba Species 0.000 claims description 83
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 238000010992 reflux Methods 0.000 claims description 30
- CWEZAWNPTYBADX-UHFFFAOYSA-N Procyanidin Natural products OC1C(OC2C(O)C(Oc3c2c(O)cc(O)c3C4C(O)C(Oc5cc(O)cc(O)c45)c6ccc(O)c(O)c6)c7ccc(O)c(O)c7)c8c(O)cc(O)cc8OC1c9ccc(O)c(O)c9 CWEZAWNPTYBADX-UHFFFAOYSA-N 0.000 claims description 29
- 229920002414 procyanidin Polymers 0.000 claims description 29
- 239000000706 filtrate Substances 0.000 claims description 25
- 239000011347 resin Substances 0.000 claims description 21
- 229920005989 resin Polymers 0.000 claims description 21
- 238000010828 elution Methods 0.000 claims description 19
- AZHSSKPUVBVXLK-UHFFFAOYSA-N ethane-1,1-diol Chemical compound CC(O)O AZHSSKPUVBVXLK-UHFFFAOYSA-N 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 239000012141 concentrate Substances 0.000 claims description 11
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 9
- 239000012535 impurity Substances 0.000 claims description 9
- 238000000926 separation method Methods 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 5
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- 235000013402 health food Nutrition 0.000 abstract description 7
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- 230000005855 radiation Effects 0.000 abstract description 3
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- JPFCOVZKLAXXOE-XBNSMERZSA-N (3r)-2-(3,5-dihydroxy-4-methoxyphenyl)-8-[(2r,3r,4r)-3,5,7-trihydroxy-2-(4-hydroxyphenyl)-3,4-dihydro-2h-chromen-4-yl]-3,4-dihydro-2h-chromene-3,5,7-triol Chemical compound C1=C(O)C(OC)=C(O)C=C1C1[C@H](O)CC(C(O)=CC(O)=C2[C@H]3C4=C(O)C=C(O)C=C4O[C@@H]([C@@H]3O)C=3C=CC(O)=CC=3)=C2O1 JPFCOVZKLAXXOE-XBNSMERZSA-N 0.000 abstract 3
- 229920001991 Proanthocyanidin Polymers 0.000 abstract 3
- 239000003814 drug Substances 0.000 abstract 1
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- 239000000203 mixture Substances 0.000 description 21
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- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 description 17
- MOJZMWJRUKIQGL-FWCKPOPSSA-N Procyanidin C2 Natural products O[C@@H]1[C@@H](c2cc(O)c(O)cc2)Oc2c([C@H]3[C@H](O)[C@@H](c4cc(O)c(O)cc4)Oc4c3c(O)cc(O)c4)c(O)cc(O)c2[C@@H]1c1c(O)cc(O)c2c1O[C@@H]([C@H](O)C2)c1cc(O)c(O)cc1 MOJZMWJRUKIQGL-FWCKPOPSSA-N 0.000 description 17
- HGVVOUNEGQIPMS-UHFFFAOYSA-N procyanidin Chemical compound O1C2=CC(O)=CC(O)=C2C(O)C(O)C1(C=1C=C(O)C(O)=CC=1)OC1CC2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 HGVVOUNEGQIPMS-UHFFFAOYSA-N 0.000 description 17
- 230000000694 effects Effects 0.000 description 16
- 238000003809 water extraction Methods 0.000 description 13
- 239000002904 solvent Substances 0.000 description 11
- 238000004090 dissolution Methods 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 241000219095 Vitis Species 0.000 description 8
- 235000009754 Vitis X bourquina Nutrition 0.000 description 8
- 235000012333 Vitis X labruscana Nutrition 0.000 description 8
- 235000014787 Vitis vinifera Nutrition 0.000 description 8
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- 239000003963 antioxidant agent Substances 0.000 description 7
- 235000006708 antioxidants Nutrition 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 6
- 230000001093 anti-cancer Effects 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 230000002195 synergetic effect Effects 0.000 description 6
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 5
- 235000005487 catechin Nutrition 0.000 description 5
- 229950001002 cianidanol Drugs 0.000 description 5
- 230000000259 anti-tumor effect Effects 0.000 description 4
- XMOCLSLCDHWDHP-IUODEOHRSA-N epi-Gallocatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC(O)=C(O)C(O)=C1 XMOCLSLCDHWDHP-IUODEOHRSA-N 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 239000000178 monomer Substances 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 240000003152 Rhus chinensis Species 0.000 description 3
- 235000014220 Rhus chinensis Nutrition 0.000 description 3
- 229930014669 anthocyanidin Natural products 0.000 description 3
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 3
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- 230000003064 anti-oxidating effect Effects 0.000 description 3
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- -1 waits under dosage Substances 0.000 description 3
- XMOCLSLCDHWDHP-UHFFFAOYSA-N L-Epigallocatechin Natural products OC1CC2=C(O)C=C(O)C=C2OC1C1=CC(O)=C(O)C(O)=C1 XMOCLSLCDHWDHP-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 239000003463 adsorbent Substances 0.000 description 2
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- 230000003796 beauty Effects 0.000 description 2
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- 239000000539 dimer Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- DZYNKLUGCOSVKS-UHFFFAOYSA-N epigallocatechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3cc(O)c(O)c(O)c3 DZYNKLUGCOSVKS-UHFFFAOYSA-N 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
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- 230000004083 survival effect Effects 0.000 description 2
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- PFTAWBLQPZVEMU-ZFWWWQNUSA-N (+)-epicatechin Natural products C1([C@@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-ZFWWWQNUSA-N 0.000 description 1
- VZRRCQOUNSHSGB-UHFFFAOYSA-N 4-hydroxy-4,6,6-trimethylbicyclo[3.1.1]heptan-3-one Chemical compound C1C2C(C)(C)C1CC(=O)C2(O)C VZRRCQOUNSHSGB-UHFFFAOYSA-N 0.000 description 1
- 244000080767 Areca catechu Species 0.000 description 1
- 235000006226 Areca catechu Nutrition 0.000 description 1
- 210000003771 C cell Anatomy 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101100370002 Mus musculus Tnfsf14 gene Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000000118 anti-neoplastic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 1
- 210000004413 cardiac myocyte Anatomy 0.000 description 1
- 210000003792 cranial nerve Anatomy 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000035617 depilation Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000012850 discrimination method Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- LPTRNLNOHUVQMS-UHFFFAOYSA-N epicatechin Natural products Cc1cc(O)cc2OC(C(O)Cc12)c1ccc(O)c(O)c1 LPTRNLNOHUVQMS-UHFFFAOYSA-N 0.000 description 1
- 235000012734 epicatechin Nutrition 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 229930184727 ginkgolide Natural products 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 150000002596 lactones Chemical class 0.000 description 1
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 150000002825 nitriles Chemical class 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 235000010603 pastilles Nutrition 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 210000001525 retina Anatomy 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/16—Ginkgophyta, e.g. Ginkgoaceae (Ginkgo family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4926—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having six membered rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
Description
提取溶剂 | 提取物得率(%) | 原花青素转移率(%) | 多糖转移率(%) |
A | 17.5 | 51.6 | 88.7 |
B | 19.8 | 69.5 | 88.8 |
C | 22.4 | 98.6 | 88.9 |
D | 19.2 | 42.6 | 85.1 |
E | 19.7 | 98.4 | 31.5 |
提取溶剂料液比 | 提取物得率(%) | 原花青素转移率(%) | 多糖转移率(%) |
6 | 20.0 | 81.4 | 63.6 |
10 | 22.7 | 95.3 | 89.9 |
15 | 22.9 | 97.2 | 91.3 |
20 | 23.1 | 97.8 | 91.7 |
提取时间(h) | 提取物得率(%) | 原花青素转移率(%) | 多糖转移率(%) |
1 | 21.1 | 88.7 | 75.2 |
2 | 22.8 | 95.6 | 89.8 |
3 | 22.8 | 95.9 | 90.2 |
提取液浓缩体积 | 提取物得率(%) | 原花青素转移率(%) | 多糖转移率(%) |
0.5倍量(50ml) | 16.4 | 83.2 | 85.5 |
1倍量(100ml) | 17.5 | 90.8 | 88.7 |
2倍量(200ml) | 17.8 | 91.1 | 89.3 |
5倍量(500ml) | 18.2 | 91.4 | 89.8 |
尿素加入量(g/L) | 提取物得率(%) | 原花青素转移率(%) | 多糖转移率(%) |
0 | 14.9 | 57.6 | 85.1 |
0.1 | 16.2 | 80.4 | 87.5 |
0.2 | 17.3 | 90.2 | 88.2 |
0.5 | 17.3 | 90.8 | 88.1 |
1.0 | 17.4 | 90.8 | 88.5 |
提取时间(h) | 提取物得率(%) | 原花青素转移率(%) | 多糖转移率(%) |
0.5 | 15.8 | 81.2 | 86.9 |
1 | 17.2 | 89.7 | 88.4 |
2 | 17.4 | 90.4 | 88.5 |
6 | 17.3 | 90.2 | 88.3 |
乙醇醇沉浓度(%) | 提取物得率(%) | 多糖含量(%) | 多糖转移率(%) |
70 | 4.86 | 86.7 | 75.2 |
75 | 5.12 | 87.6 | 80.1 |
80 | 5.43 | 88.1 | 85.4 |
85 | 5.59 | 86.5 | 86.3 |
90 | 5.70 | 86.3 | 87.8 |
溶解沉淀用水量 | 提取物得率(%) | 多糖含量(%) | 多糖转移率(%) |
0.5倍量 | 4.77 | 94.2 | 80.2 |
1倍量 | 4.93 | 96.7 | 85.1 |
2倍量 | 4.97 | 96.5 | 85.6 |
5倍量 | 4.99 | 96.2 | 85.8 |
10倍量 | 5.01 | 95.9 | 85.8 |
水温(℃) | 提取物得率(%) | 多糖含量(%) | 多糖转移率(%) |
50 | 4.53 | 94.2 | 76.2 |
70 | 4.74 | 94.7 | 80.1 |
90 | 4.98 | 96.1 | 85.4 |
100 | 4.98 | 96.3 | 85.6 |
树脂型号 | 提取物得率(%) | 原花青素含量(%) | 原花青素转移率(%) |
AB-8 | 5.24 | 75.2 | 87.6 |
HPD-750 | 5.55 | 70.9 | 87.4 |
HPD-200A | 5.16 | 74.3 | 85.2 |
HPD-100C | 4.56 | 85.2 | 86.3 |
HPD-417 | 4.21 | 86.1 | 80.5 |
ADS-17 | 3.90 | 93.1 | 80.7 |
洗脱部位 | 提取物得率(%) | 原花青素含量(%) | 原花青素转移率(%) |
水 | 7.63 | 0.3 | 0.5 |
5%乙醇 | 0.24 | 22.5 | 1.2 |
10%乙醇 | 0.23 | 93.9 | 4.8 |
20%乙醇 | 0.81 | 96.1 | 17.3 |
30%乙醇 | 1.21 | 95.6 | 25.7 |
50%乙醇 | 1.69 | 95.9 | 36 |
70%乙醇 | 0.19 | 4.7 | 0.2 |
Claims (10)
Priority Applications (1)
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CN201510454258.3A CN106389180B (zh) | 2015-07-29 | 2015-07-29 | 一种银杏原花青素-多糖混合提取物及其制备方法和应用 |
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CN201510454258.3A CN106389180B (zh) | 2015-07-29 | 2015-07-29 | 一种银杏原花青素-多糖混合提取物及其制备方法和应用 |
Publications (2)
Publication Number | Publication Date |
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CN106389180A true CN106389180A (zh) | 2017-02-15 |
CN106389180B CN106389180B (zh) | 2018-12-11 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108403574A (zh) * | 2018-06-08 | 2018-08-17 | 桃源县兴隆米业科技开发有限公司 | 一种基于紫米提取液美白保湿面膜的制备方法 |
CN114957185A (zh) * | 2022-05-31 | 2022-08-30 | 安徽大学绿色产业创新研究院 | 一种提高花青素稳定性的方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001078783A2 (en) * | 2000-04-17 | 2001-10-25 | Hauser, Inc. | Compositions comprising natural agents for treatment of cancer |
CN101845035A (zh) * | 2009-03-24 | 2010-09-29 | 上海医药工业研究院 | 一种低聚原花青素的提取方法 |
CN102558128A (zh) * | 2011-11-28 | 2012-07-11 | 浙江康恩贝制药股份有限公司 | 含银杏原花青素提取物及其制备方法和应用 |
CN103130816A (zh) * | 2012-10-19 | 2013-06-05 | 湖北诺克特药业有限公司 | 一种从银杏叶中制备多种活性物质的方法 |
CN104784370A (zh) * | 2015-04-30 | 2015-07-22 | 陈大忠 | 一种能够清除自由基的抗氧化药物组合物及其制备方法 |
-
2015
- 2015-07-29 CN CN201510454258.3A patent/CN106389180B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001078783A2 (en) * | 2000-04-17 | 2001-10-25 | Hauser, Inc. | Compositions comprising natural agents for treatment of cancer |
CN101845035A (zh) * | 2009-03-24 | 2010-09-29 | 上海医药工业研究院 | 一种低聚原花青素的提取方法 |
CN102558128A (zh) * | 2011-11-28 | 2012-07-11 | 浙江康恩贝制药股份有限公司 | 含银杏原花青素提取物及其制备方法和应用 |
CN103130816A (zh) * | 2012-10-19 | 2013-06-05 | 湖北诺克特药业有限公司 | 一种从银杏叶中制备多种活性物质的方法 |
CN104784370A (zh) * | 2015-04-30 | 2015-07-22 | 陈大忠 | 一种能够清除自由基的抗氧化药物组合物及其制备方法 |
Non-Patent Citations (1)
Title |
---|
苏亮等: "银杏枝皮化学成分的研究", 《药物生物技术》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108403574A (zh) * | 2018-06-08 | 2018-08-17 | 桃源县兴隆米业科技开发有限公司 | 一种基于紫米提取液美白保湿面膜的制备方法 |
CN108403574B (zh) * | 2018-06-08 | 2021-07-23 | 桃源县兴隆米业科技开发有限公司 | 一种基于紫米提取液美白保湿面膜的制备方法 |
CN114957185A (zh) * | 2022-05-31 | 2022-08-30 | 安徽大学绿色产业创新研究院 | 一种提高花青素稳定性的方法 |
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