CN107325980A - A kind of radiation hardness series bacillus KH9 and its application in biological antitranspirant - Google Patents
A kind of radiation hardness series bacillus KH9 and its application in biological antitranspirant Download PDFInfo
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- CN107325980A CN107325980A CN201710438887.6A CN201710438887A CN107325980A CN 107325980 A CN107325980 A CN 107325980A CN 201710438887 A CN201710438887 A CN 201710438887A CN 107325980 A CN107325980 A CN 107325980A
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Abstract
The present invention discloses a kind of radiation hardness series bacillus KH9 and its application in biological antitranspirant, by separation, screening, culture in the pedotheque that Xinjiang Lop Nur periphery arid-desert areas is gathered, one plant of production polysaccharide ability is obtained higher so as to have the radiation hardness series bacillus of reduction plant transpiration effect（Paenibacillus sp.）KH9 CGMCC No. 12799, there are obvious physio-biochemical characteristics difference and the otherness of molecular level with common series bacillus strain, when the radiation hardness series bacillus bacterial strain that the numbering filtered out is KH9 is applied with antitranspirant compounding in drought resisting, in crop leaf formation biofilm, the transpiration of crop can effectively be reduced, strengthen photosynthesis, chlorophyll content improves 4.50%, there is stronger growth ability, osmotic adjustment ability and active oxygen defence capability to crop, destructiveness of the crop by drought stress can be reduced.
Description
Technical field
The invention belongs to microbial technology field, it is related to a kind of radiation hardness series bacillus novel bacterial and its in the anti-steaming of biology
Rise the technical field of the application in agent.
Technical background
With the aggravation of greenhouse effects, trend of drought will further continue.At present, there is 1/3 soil to be in the world to do
Non-irrigated, semiarid state, the arable land that China also has 48.9% is in arid, semiarid zone.Drought stress is as a kind of main
Abiotic stress, seriously governs the sustainable development of agricultural, will cause that plant growth is slow, yield reduction, pest and disease damage are aggravated
Etc. phenomenon, it is therefore necessary to develop efficient drought-resistant water saving measures.
In recent years, chemical water-saving technology is applied in coping with agriculture drought more and more, and the use of antitranspirant is exactly wherein
One of relatively effective measure.Antitranspirant sprays scattered in transpiration rate, the reduction moisture that after plant leaf surface, can reduce plant
Lose, and by influenceing enzymatic protective system activity to improve the drought resistance of plant.Studies have shown that using existing anti-transpiration
Agent can cause blade face stomata closing and then photosynthesis weakens, and net photosynthesis product is reduced, and then plant growth slows down.Therefore need
A kind of new antitranspirant is developed, disadvantages mentioned above is overcome, makes energy more efficient land productivity of the antitranspirant in coping with agriculture drought production
With.
The drought resistance function of microorganism is in addition to by adjusting own metabolism activity induction plant generation resistance, certain micro-organisms production
Polysaccharide material, due to its special Polymer Structure, can leaf surface formation with the extracellular compound of cell (EPS) for it is main into
Point biofilm induce stomatal response, reduction plant transpiration effect improves WUEL, to resist water deficit,
Play the function such as retain water and nutrients and improved soil structure.Microorganism and antitranspirant are subjected to the compounding research and development anti-transpiration of new bio
Agent, is retaining its each on the basis of advantage, will can give play to more efficient, more reliable effect, but is compounded pair on both at present
The report of the synergy of plant drought is seldom, it is therefore desirable to further probe into the drought resistance function of microorganism, is microorganism drought-resisting
There is provided with the effect of reduction plant transpiration and the stable resistant strain of performance, there will be potential application and development in plant drought field
Prospect.
The content of the invention
For having no in the prior art about the radiation hardness series bacillus novel bacterial in the present invention and in biological anti-transpiration
The relevant report of application in agent, and existing antitranspirant can cause blade face stomata closing to weaken photosynthesis, so that making
The present situation of thing decreased growth, it is contemplated that for offer is with the effect of reduction plant transpiration and performance is stable in biological antitranspirant
Resistant strain.The present invention has by separating one plant of radiation hardness series bacillus novel bacterial in pedotheque
Produce polysaccharide ability higher so as to significantly reduction plant transpiration effect.By providing a kind of radiation hardness series bacillus
(Paenibacillus sp.) KH9CGMCC No.12799, and using the bacterium with the method for antitranspirant compounding and in drought resisting
In application technology scheme.
The main technical schemes that the present invention is used：
Pedotheque of the present invention picks up from Xinjiang Lop Nur periphery arid by Microorgan Application Inst., Xinjiang Agricultural Academy
Desert Area, after the pedotheque of collection is irradiated through 5000KGy cobalt sources, using PDA culture medium as isolation medium, largely
Screen, preferably go out a collection of well-grown, the sticky radiation hardness bacterium bacterial strain for being difficult to provoke, carry out the survey of 16S rRNA gene orders
It is fixed, therefrom filter out one plant of higher radiation hardness series bacillus novel bacterial (Paenibacillus sp.) of production polysaccharide ability.
The present invention specifically provides a kind of radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC
No.12799, by providing the screening technique determined, separation screening and culture in the pedotheque of collection obtain a collection of class
Micro-organisms bacillus bacterial strain, therefrom filters out one plant of higher series bacillus bacterial strain KH9 of production polysaccharide ability, through microbiology
Classification and identification, belong to series bacillus (Paenibacillus sp.) bacterial strain, are temporarily named as Paenibacillus
sp.KH9。
Specifically, after the present invention to collection pedotheque through 5000KGy cobalt sources by irradiating, being separated, being screened
And culture, the bacterial strain that one plant of numbering is KH9 is therefrom filtered out, through microbiological classification and identification, the bacterial strain belongs to class gemma bar
Bacterium (Paenibacillus sp.) bacterial strain.The bacterial strain is in being preserved in budapest treaty microorganism international accession before the applying date
Unit：China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC).Address：The Chaoyang District, Beijing City North Star
The institute 3 of West Road 1, Institute of Microorganism, Academia Sinica, postcode：100101.Preservation date on July 20th, 2016, strain is protected
Tibetan number is CGMCC No.12799.Series bacillus (Paenibacillus sp.) KH9 is accredited as through microbiology.The bacterial strain
Optimum growing condition is：30 DEG C of temperature, culture medium uses PDA culture medium (potato 200g/L, glucose 20g/L, agar 15g/
L, distilled water 1L, pH are natural), incubation time 3d；After being cultivated through 3d, on PDA culture medium surface, bacterium colony is rounded, neat in edge,
It is raised, smooth, pink, opaque, sticky to be difficult to provoke；The bacterium be gram-positive bacteria, aerobic, motility, thalline it is shaft-like,
Peritrichous, there is endospore to be formed.According to above morphological feature, reference《Primary Jie Shi systematic bacteriologies identification handbook》8th edition
With《Conventional bacterial system identification handbook》Morphology, Physiology and biochemistry identification, and binding molecule biology is carried out to KH9 bacterial strains to survey
Sequence, Preliminary Identification bacterial strain is the member of series bacillus (Paenibacillus sp.), but the strain has novel bacterial distinct
Feature, from its taxology angle, is temporarily named as radiation hardness Ulocladium fungi Paenibacillus sp.KH9.
Meanwhile, the STb gene of the invention by extracting bacterial strain KH9 draws using using bacterial 16 S rDNAPCR amplifications are general
Thing, enters performing PCR amplification, and PCR primer is sequenced after cutting glue purification.By surveyed 16S rRNA gene orders and GenBank databases
In sequence be compared, as a result show：Bacterial strain KH9 and type strain Paenibacillusxylanexedens NRRL B-
Homologous 1090T (CLG48537) maximums are 97.3%, be respectively less than 97.0% with belonging to other bacterial strain homologys together, it is not can determine that still
Definite classification, is defined as one plant of novel bacterial, Paenibacillus sp.KH9 is temporarily named as from its taxology angle.
Further, the present invention provides a kind of radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC
No.12799 is compounded and the application in drought resisting with antitranspirant.The antitranspirant that the present invention is used is with camel thorn alkalescence essence
Oil, fulvic acid are primary raw material, by chemical washing, purification, activating process, and production is rich in amino acid, proline, abscisic acid
And the antitranspirant of the big-and-middle element such as micro, KH9 thalline are compounded when using.The strain substantially has the rising work of reduction crop
With enhancing photosynthesis dramatically increases chlorophyll content, strengthens plant enzyme system physiologically active, stimulates plant root growth.
By implementing particular technique index of the present invention, present invention is realized, following beneficial effect can be reached：
(1) radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799 that the present invention is provided,
Simple with condition of culture, breeding is fast, and hereditary capacity is stable, the higher advantage of production polysaccharide ability.
(2) radiation hardness series bacillus (Paenibacillus sp.) the KH9CGMCC No.12799 that provide of the present invention with
When antitranspirant compounding is applied in drought resisting, in crop leaf formation biofilm, the transpiration of crop can be effectively reduced, is increased
Strong photosynthesis, chlorophyll content improves 4.50%, has stronger growth ability, osmotic adjustment ability and activity to crop
Oxygen defence capability, can reduce destructiveness of the crop by drought stress.
Brief description of the drawings
Fig. 1 show radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799 bacterium colony and
Thalline photo.
Fig. 2 show radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799 systems
System development dendrogram.
Fig. 3 show the life based on radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799
Influence of the thing antitranspirant to Wheat Seedlings under Drought Stress leaf r elative water content.
Fig. 4 show the life based on radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799
Influence of the thing antitranspirant to Wheat Seedlings under Drought Stress proline content.
Fig. 5 show the life based on radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799
Influence of the thing antitranspirant to Wheat Seedlings under Drought Stress leaf malondialdehyde content.
Fig. 6 show the life based on radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799
Influence of the thing antitranspirant to Wheat Seedlings under Drought Stress blade superoxide dismutase (SOD) activity.
Embodiment
Below, the present invention is illustrated for embodiment, still, the present invention is not limited to following embodiments.Selected in the present invention
All raw and auxiliary materials, and the Spawn incubation method selected all is well known in the art selection, the % being related in the present invention
All be weight percentage, unless otherwise indicated except.
Embodiment one：Radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799 separation,
Screening and identification
1st, the separation and screening of strain
Series bacillus (Paenibacillus sp.) KH9 used in the present invention is by Xinjiang Agricultural Sciences institute microorganism
Applied Research Laboratory from Xinjiang Lop Nur periphery arid-desert areas largely gather soil in sample, through 5000KGy cobalt sources carry out spoke
According to rear separation, the bacterium of the production polysaccharide in soil layer, plate streak purifying bacterial strain, warp are isolated using traditional plating method
Cross optimal screening and go out the strong bacterium bacterial strain of a collection of well-grown, production polysaccharide ability, therefrom preferably go out the bacterium that one plant of numbering is KH9
Strain.
Separating step：
(1) preparation of soil sample dilution：By through the irradiated soil sample of 5000KGy cobalt sources, it is dissolved according to the ︰ 10 of solid-liquid ratio 1
In triangular flask containing sterilized water, 30 DEG C of constant temperature oscillation (160r/min) culture 30min, take out triangular flask and are placed in after glass bar stirring
Horizontal table top stands 30min.1mL supernatants are taken in the test tube equipped with the aqua sterilisa containing 9mL, are fully mixed, by gradient dilution method
10 are diluted to step by step-4。
(2) isolate and purify：Take 10-2、10-3、10-4Each 1mL of dilution is coated on PDA solid mediums, and glass is used immediately
Spatula is smeared uniform.Flat board is inverted in 30 DEG C of constant incubator culture 5d.After bacterium colony is grown, picking colony surface is smooth,
It is sticky, be difficult the bacterium single bacterium colony that lifts, continue to isolate and purify using method of scoring, until obtaining pure culture.
2nd, the condition of culture of bacterial strain
(1) growth medium for the bacterial strain that numbering is KH9 is PDA culture medium：Potato 200g/L, glucose 20g/L, fine jade
Fat 15g/L, distilled water 1L, pH through 30 DEG C, 96h naturally, cultivate.
(2) bacterial strain that numbering is KH9 can grow under the conditions of 20-40 DEG C, and optimum growth temperature is 30 DEG C, incubation time
For 3-5d.
(3) the bacterial strain the most suitable growth pH7 that numbering is KH9.
Specifically, after the present invention to collection pedotheque through 5000KGy cobalt sources by irradiating, being separated, being screened
And culture, the bacterial strain that one plant of numbering is KH9 is therefrom filtered out, through microbiological classification and identification, the bacterial strain belongs to class gemma bar
Bacterium (Paenibacillus sp.) bacterial strain.The bacterial strain is in being preserved in budapest treaty microorganism international accession before the applying date
Unit：China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC).Address：The Chaoyang District, Beijing City North Star
The institute 3 of West Road 1, Institute of Microorganism, Academia Sinica, postcode：100101.Preservation date on July 20th, 2016, strain is protected
Tibetan number is CGMCC No.12799.The novel bacterial of series bacillus is accredited as through microbiology, is divided from its taxology angle is tentative
Class is series bacillus (Paenibacillus sp.) KH9.The bacterial strain optimum growing condition is：30 DEG C of temperature, culture medium is used
PDA culture medium (potato 200g/L, glucose 20g/L, agar 15g/L, distilled water 1L, pH are natural), incubation time 3d.
3rd, bacterial strain KH9 Physiology and biochemistry identification
Morphological feature：By the streak inoculation of KH9 bacterial strains in being cultivated on PDA plate after 3d, bacterium colony is rounded, neat in edge, convex
Rise, smooth, pink, opaque, sticky be difficult to provoke；The bacterium is that gram-positive bacteria, aerobic, motility, thalline be shaft-like, week
Give birth to flagellum, there is endospore to be formed, its bacterium colony and thalli morphology are referring to accompanying drawing 1.
Physiological and biochemical property：Biolog GN2 plates detect that the available carbon source of the bacterium is：Cyclodextrin, dextrin, mannosan,
Polysorbate40, Tween 80, acetic acid, gamma-hydroxybutyric acid, α-ketoglutaric acid, pyruvic acid, succinamic acid, butanedioic acid, 2- deoxidation glands
Glycosides, inosine and D- Lip rivers ketose.
Pass through above-mentioned strain radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799 bacterium
Volume morphing, cultural characteristic observation and Determination of Physiological And Biochemical Indices, i.e., observed by thalli morphology, strain culturing observation of characteristics, life
Long temperature measuring etc. is tested, reference《Primary Jie Shi systematic bacteriologies identification handbook》8th edition and《Conventional bacterial system identification handbook》
Method carry out, the strain that numbering is KH9 is poor with obvious physio-biochemical characteristics compared with common series bacillus strain
It is different, and the ability of production antibiotic and polysaccharide is stronger, it is a kind of typical novel bacterial to show KH9 bacterial strains, from its taxology angle, should
The member of bacterial strain ownership series bacillus (Paenibacillus sp.), is temporarily named as radiation hardness Ulocladium fungi
Paenibacillussp.KH9。
Embodiment two：Radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799 molecular levels
Identification
Bacterial strain KH9 STb gene is extracted, universal primer is expanded using using bacterial 16 S rDNA PCR, enters performing PCR amplification,
PCR primer is sequenced after cutting glue purification.Known array in the gained sequence of experimental strain and GenBank databases is carried out
BLAST compares, it is determined that the race relation nearest with experimental strain affiliation.And combine EzTaxon (http://
Www.ezbiocloud.net/eztaxon sequence alignment and associative mode strain sequence is transferred in), to carry out phylogenetic tree
Analytical standard pattern bacterium sequence, is carried out using the software kits of MEGA 5.0 using adjacent method (Neighbor-Joining method)
Clustering and systematic evolution tree are built.It can be shown by systematic evolution tree, bacterial strain KH9 and type strain Paenibacillus
Homologous xylanexedens NRRL B-1090T (CLG48537) maximums are 97.3%, small with belonging to other bacterial strain homologys together
In 97.0%, systematic evolution tree is referring to accompanying drawing 2, strain that numbering the is KH9 series bacillus common with it
(Paenibacillus sp.) member bacterium has distinct difference, with obvious molecular level otherness, is defined as class gemma
The novel bacterial of bacillus, from its taxology angle, the bacterial strain belongs to the member of series bacillus (Paenibacillus sp.), temporarily
It is named as Paenibacillus sp.KH9.
Embodiment three：The system of radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799 thalline
It is standby
By activated bacterial strain radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC of the present invention
No.12799 is inoculated in the seed test tube equipped with 5ml PDA liquid mediums, in 30 DEG C of cultures, 200rpm shaken cultivations 36h
Afterwards, it is inoculated in PDA liquid medium that (amount of adding is the bottled 100ml PDA Liquid Cultures of 500ml triangles by 2% inoculum concentration
Base), in 30 DEG C of cultures, 200rpm shake flask fermentations 3-4 days, living bacteria count >=200,000,000/ml.
Example IV：Life based on radiation hardness series bacillus (Paenibacillus sp.) KH9CGMCC No.12799
Application of the thing antitranspirant in drought resisting
1. application method：
The composition that antitranspirant is included：Liquid fulvic acid >=80g/L, N P and K >=40g/L, camel thorn essential oil >=80g/
L, amino acid >=5g/L.
Compounding：Using preceding by 500 times of antitranspirant dilution, the amount of spraying of bacterium solution is the antitranspirant weight after dilution
20%.
2. pot experiment
The present invention uses method for potted, is desert grey soil for examination soil.Remove and sieved after stone and grass roots, and add urea,
Double superhosphate and potassium sulfate (are 200mg/kg, N according to amount of nitrogen as base fertilizer:P2O5:K2O=18:20:8 calculate), fully
Mix thoroughly.Experiment sets 3 irrigation quantity levels, is respectively：Adequate water supply, breeding time soil moisture content maintains field capacity
75%；Moderate water stress, breeding time soil moisture content maintains the 65% of field capacity；Severe water stress, breeding time
Soil moisture content maintains the 55% of field capacity.Each irrigation quantity level divides into 3 processing and is respectively：CK：Without anti-
Rising agent and microbial inoculum, T1：50 times of dilution antitranspirants, T2：The microbial bacteria of 50 times of dilution antitranspirants+20%.
Water is respectively adopted in CK, T1, T2 processing, and 50 times of dilution antitranspirants, microbial inoculums of 50 times of dilution antitranspirants+20% are mixed
Vexed 30min after wheat seed, is sowed, adequate water supply after then drying.Soil water content is controlled when 3 leaf phase, using difference
Drought resisting is handled, and is uniformly sprayed on wheat leaf blade.Spray latter every two days the consumed moisture of supplement.
Radiation hardness series bacillus 3. (Paenibacillus sp.) KH9CGMCC No.12799 biology antitranspirants pair
The influence of the lower wheat leaf blade relative water content of different in moisture stress
The measure of the relative water content of wheat leaf blade, as a result as shown in Figure 3, the bar of gemma containing class are carried out after spraying 5 days
The biological antitranspirant of bacterium novel bacterial is handled in 55% moisture condition lower blade relative water content higher than antitranspirant is singly applied
15.23%.Contain it can be seen that the biological antitranspirant of the novel bacterial containing series bacillus can strengthen the relative of plant leaf blade
Water, reduces the rising ability of blade.
Radiation hardness series bacillus 4. (Paenibacillus sp.) KH9CGMCC No.12799 biology antitranspirants pair
The influence of the lower wheat seedling growth of different in moisture stress
Under moderate water stress's (65%) and severe water stress (55%), spray containing the biological anti-transpiration of series bacillus
Growth and chlorophyll content of the agent to wheat seedling have certain facilitation, as a result as shown in table 1：
Table 1：Influence of the biological antitranspirant of different disposal to wheat seedling growth
As shown in Table 1, compared with singly applying the processing of antitranspirant, under 65% moisture condition, the wheat of KH9 thalline is compounded
Seedling Biomass, which improves 11.86%, plant height and improves 2.35%, chlorophyll content, improves 4.50%；In 55% moisture bar
Under part, T1 and T2 processing biomass difference is not notable.
Radiation hardness series bacillus 5. (Paenibacillus sp.) KH9CGMCC No.12799 biology antitranspirants pair
The influence of the lower wheat seedlings blade proline (PRO) of different in moisture stress and MDA (MDA)
From accompanying drawing 4 as can be seen that compounding the anti-transpiration of series bacillus novel bacterial under 65% and 55% two moisture condition
The processing that agent processing wheat seedlings blade proline (PRO) content more singly applies antitranspirant reduces 11.08% He respectively
16.93%.
It is can be seen that from accompanying drawing 5 compared with singly applying the processing of antitranspirant, the antitranspirant of the novel bacterial containing series bacillus
Processing wheat seedlings blade MDA (MDA) content under 55% moisture condition is reduced under 10.48%, but 65% moisture condition
Raise on the contrary.
Radiation hardness series bacillus 6. (Paenibacillus sp.) KH9CGMCC No.12799 biology antitranspirants pair
The influence of the lower wheat seedlings blade superoxide dismutase (SOD) of different in moisture stress
It is can be seen that from accompanying drawing 6 compared with singly applying the processing of antitranspirant, the antitranspirant of the novel bacterial containing series bacillus
The activity 5.00% of the SOD enzymes under 55% moisture condition is handled, but is raised under 65% moisture condition.
In summary experiment is understood, the radiation hardness series bacillus (Paenibacillus sp.) that the present invention is provided
KH9CGMCC No.12799 are developed into biological antitranspirant, under the conditions of low moisture, can effectively be dropped relative to antitranspirant is singly applied
The transpiration of low crop, strengthens photosynthesis, chlorophyll content improves 4.50%, while having stronger growth to crop
Ability, osmotic adjustment ability and active oxygen defence capability, can improve destructiveness of the crop by drought stress.
Above-described embodiment is only intended to clearly illustrate example of the present invention, and the not restriction to embodiment.
For those of ordinary skill in the field, other various forms of changes can also be made on the basis of the above description
Or change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended
Or among changing still in protection scope of the present invention.
SEQUENCE LISTING
<110>Microorgan Application Inst., Xinjiang Agricultural Academy（In Xinjiang, China-Armenia's bio-engineering research exploitation
The heart）
<120>A kind of radiation hardness series bacillus KH9 and its application in biological antitranspirant
<130> KH9
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1428
<212> DNA
<213> KH9
<220>
<221> KH9
<222> (1)..(1428)
<400> 1
ctggccaggt cgagcggagt tgataggaag cttgcttcct tgatacttag cggcggacgg 60
gtgagtaaca cgtaggcaac ctgccctcaa gtttgggaca actaccggaa acggtagcta 120
ataccgaata attgttttct tcgcctgaag ggaactggaa agacggagca atctgtcact 180
tggggatggg cctgcggcgc attagctagt tggtgaggta acggctcacc aaggcgacga 240
tgcgtagccg acctgagagg gtgatcggcc acactgggac tgagacacgg cccagactcc 300
tacgggaggc agcagtaggg aatcttccgc aatgggcgaa agcctgacgg agcaatgccg 360
cgtgagtgat gaaggttttc ggatcgtaaa gctctgttgc cagggaagaa cgcttgggag 420
agtaactgct ctcaaggtga cggtacctga gaagaaagcc ccggctaact acgtgccagc 480
agccgcggta atacgtaggg ggcaagcgtt gtccggaatt attgggcgta aagcgcgcgc 540
aggcggtcat ttaagtctgg tgtttaatcc cggggctcaa ccccggatcg cactggaaac 600
tgggtgactt gagtgcagaa gaggagagtg gaattccacg tgtagcggtg aaatgcgtag 660
atatgtggag gaacaccagt ggcgaaggcg actctctggg ctgtaattga cgctgaggcg 720
cgaacgcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag 780
tgctaggtgt taggggtttc gatacccttg gtgccgaagt taacacatta agcactccgc 840
ctggggagta cggtcgcaag actgaaactc aaaggaattg acggggaccc gcacaagcag 900
tggattatgt gatttaattc gaagcaacgc gaagaacctt accaggtctt gacatcactc 960
tgatcgatac agagatgtat cttttccttc gggaacagcg agacaggtgg tgcatggttg 1020
tcgtcagctc gtgtcgtgag atgttgggtt aagtacccgc aacgagcgca acccttctat 1080
ttagtagcca gcacttccgg gtgggtcact cttagatgga ctgctgatga caaactgaag 1140
tacggtcgcg gatgacgtca aatcatcatg tcccttatga cctgggctac acacgtacta 1200
caatggccgg tacaacgggc agtgaagcag cgaggtggaa ccaatcctaa aaagccggtc 1260
tcagttcgga ttgcaggctg caactcgcct gcatgaagtc ggaattgcta gtaatcgcgg 1320
atcagcatgc cgcggttaat acgttcccgg gtcttgtaca caccgcccgt ggcaccatga 1380
gagtttataa cacccgaagc cggtaaggta accgcaagga gccatccg 1428
Claims (6)
1. a kind of radiation hardness series bacillus（Paenibacillus sp.）KH9, it is characterised in that described radiation hardness class bud
Spore bacillus（Paenibacillus sp.）KH9 CGMCC preserving numbers are No. 12799.
2. a kind of radiation hardness series bacillus as claimed in claim 1（Paenibacillus sp.）KH9, it is characterised in that
Described radiation hardness series bacillus（Paenibacillus sp.）KH9 optimum growing conditions are：30 DEG C of temperature, culture medium is adopted
With PDA culture medium, incubation time 3d.
3. a kind of radiation hardness series bacillus as claimed in claim 2（Paenibacillus sp.）KH9, it is characterised in that
Described radiation hardness series bacillus（Paenibacillus sp.）KH9 the most suitable growth culture mediums use PDA culture medium for Ma Ling
Potato 200g/L, glucose 20g/L, agar 15g/L, distilled water 1L, pH are natural.
4. a kind of radiation hardness series bacillus as claimed in claim 1（Paenibacillus sp.）KH9, it is characterised in that
Described radiation hardness series bacillus（Paenibacillus sp.）KH9 bacterium colonies are rounded, neat in edge, projection, smooth, powder
It is red, opaque, sticky to be difficult to provoke.
5. a kind of radiation hardness series bacillus as claimed in claim 1（Paenibacillus sp.）KH9, it is characterised in that
Described radiation hardness series bacillus（Paenibacillus sp.）KH9 is gram-positive bacteria, aerobic, motility, thalline bar
Shape, peritrichous, there is endospore to be formed.
6. a kind of radiation hardness series bacillus（Paenibacillus sp.）Applications of the KH9 in biological antitranspirant.
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| CN108902182A (en) * | 2018-09-28 | 2018-11-30 | 常州谙美生物科技有限公司 | A kind of probiotics and preparation method thereof with reduction plant transpiration action function |
| CN111727847A (en) * | 2020-07-08 | 2020-10-02 | 浙江省农业科学院 | Straw fiber seedling culture substrate for colonization biocontrol bacteria, production method thereof and two-step colonization biocontrol bacteria method |
| CN114181850A (en) * | 2021-11-18 | 2022-03-15 | 新疆农业科学院微生物应用研究所(中国新疆—亚美尼亚生物工程研究开发中心) | UV radiation resistant bacillus and application thereof |
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| CN108902182A (en) * | 2018-09-28 | 2018-11-30 | 常州谙美生物科技有限公司 | A kind of probiotics and preparation method thereof with reduction plant transpiration action function |
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| CN111727847A (en) * | 2020-07-08 | 2020-10-02 | 浙江省农业科学院 | Straw fiber seedling culture substrate for colonization biocontrol bacteria, production method thereof and two-step colonization biocontrol bacteria method |
| CN114181850A (en) * | 2021-11-18 | 2022-03-15 | 新疆农业科学院微生物应用研究所(中国新疆—亚美尼亚生物工程研究开发中心) | UV radiation resistant bacillus and application thereof |
| CN114181850B (en) * | 2021-11-18 | 2023-01-31 | 新疆农业科学院微生物应用研究所(中国新疆—亚美尼亚生物工程研究开发中心) | UV radiation resistant bacillus and application thereof |
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Effective date of registration: 20210207 Address after: 831100 No.2 company of 105th regiment, Hutubi County, Changji Hui Autonomous Prefecture, Xinjiang Uygur Autonomous Region Patentee after: Xinjiang wankangchengyi fertilizer Co.,Ltd. Address before: 830091 No. 403 Nanchang Road, Shaibak District, Urumqi City, Xinjiang Uygur Autonomous Region Patentee before: INSTITUTE OF MICROBIAL APPLICATIONS, XINJIANG ACADEMY OF AGRICULTURAL SCIENCES (CHINA XINJIANG-ARMENIA BIOENGINEERING RESEARCH AND DEVELOPMENT CENTER) |
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