CN107129952A - A kind of lactic acid bacteria composite fungicide of raising salt-soda soil peanut yield character and its application - Google Patents
A kind of lactic acid bacteria composite fungicide of raising salt-soda soil peanut yield character and its application Download PDFInfo
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Abstract
The invention provides the lactic acid bacteria composite fungicide and its application of a kind of raising salt-soda soil peanut quality and yield traits, the active bacteria in the lactic acid bacteria composite fungicide is made up of Bacillus bifidus V9 (Bifidobacterium animalis subsp.Lactis V9), Lactobacillus plantarum (Lactobacillus plantarum) P 8 and Lactobacillus casei (Lactobacillus casei) Zhang.The lactic acid bacteria composite fungicide has obvious influence to the growth metabolism of saline and alkaline tibet milkwort root, it is particularly possible to improve the quality and yield character of saline and alkaline tibet milkwort root, so that the improvement and yield for salt-soda soil peanut quality provide ideal bacterial manure raw material.
Description
Technical field
The lactic acid bacteria composite fungicide of salt-soda soil peanut quality and yield traits can be significantly improved the present invention relates to a kind of, especially
It is related to Bacillus bifidus V9 (Bifidobacterium animalis subsp.Lactis V9), Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang and and its metabolism
The lactic acid bacteria composite fungicide of product composition, belongs to microbial technology field.
Background technology
Peanut also known as peanut, dicotyledon, vein are netted veins, and seed has peanut fruit coating.Once cry in history
Peanut, beans, fallen flowers ginseng, landing pine, Cheng Shouguo, kind beans, fig, really, Chinese's beans.Peanut is longer than nourishing help, has
Help promote longevity, thus it is among the people also known as " peanut ", and be described as soya bean " Vegetable meat ", " meat or fish in element ".Flower
Life is one of big oil crops in the world five, be eat, the industrial crops for the dual-purpose that extracts oil, occupy in world's agricultural production and trade
Critical role, peanut is also important oil crops and industrial crops, and its oil content is up to 50%, best in quality, smell delicate fragrance,
Cultivated area is only second to rape in oil crops, and total output occupies first, and its byproduct peanut meal is rich in albumen, can use
In fowl, poultry and aquaculture feed, there is very important status in China.Therefore, the development of peanut industry is for increase agriculture
The people take in and ensured that national oil plant is significant safely.
Saline Land has become a global resources and environment problems.According to statistics, global saline alkali land area has been
Up to 9.55 × 108hm2.China's salinization of soil phenomenon is also increasingly severe, endless according to UNESCO and food and agricultural organization
Full statistics, China's saline alkali land area about 9.91 × 107hm2, it is mostly in state to be developed.The medium salt tolerant crop of Arachis, tool
Drought resisting, the resistance to characteristic such as lean, can develop salt-soda soil peanut production to improving salt as one of replacement crop of saline-alkali soil area cotton
Alkaline earth area agricultural planting structure, raising farmers' income, guarantee grain and oil safety supply are significant.
However, the problem soils environment in salt-soda soil is seriously damaged in growth of the Peanut Root System in soil, salinized soil
Various trace elements are not enough, trigger Peanut Crop growth seedling carefully modest, and salinized soil earth's surface is hardened, causes peanut fruit
Head is smaller, yield reduction.At present, the effective technology means of salt-soda soil peanut yield increasing are not solved still.
The content of the invention
It is an object of the invention to by probing into different lactic acid bacterias to the yield traits of peanut and the influence of quality, it is determined that suitable
The compound lactobacillus preparation preferably applied in peanut, so that salt-soda soil peanut quality and yield traits can be significantly improved by providing one kind
Lactic acid bacteria compound formulation and its application.
In order to realize the purpose of the present invention, inventor is finally obtained one kind by lot of experiments research and unremitting effort
Mix bacterium agent, the microbial inoculum, which is formulated into after bacterium solution, to be poured in peanut root, is remarkably improved salt-soda soil peanut quality and yield
Shape.Specifically, technical side's overview of the invention is as follows：
In the lactic acid bacteria composite fungicide of a kind of raising salt-soda soil peanut quality and yield traits, the lactic acid bacteria composite fungicide
Active bacteria is by Bacillus bifidus V9 (Bifidobacterium animalis subsp.Lactis V9), Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang compositions.
It should be noted that Bacillus bifidus V9 (Bifidobacterium animalis of the present invention
Subsp.Lactis V9) be isolated acidproof and bile tolerance from healthy Mongols children enteron aisle probiotics, can suppress
Pernicious bacteria, protection intestinal mucosal barrier, metabolism produce organic acid, have very high survival rate in pH 2.0-8.0 environment.
The bacterium specific name is bifidobacterium animalis subspecies (Bifidobacterium animalis subsp.lactis), bacterial strain
Protected on November 18th, 2011 in China Committee for Culture Collection of Microorganisms's General Microbiological Culture collection preservation
Tibetan number is CGMCC No.5470.
Lactobacillus plantarum (Lactobacillus plantarum) P-8 of the present invention, is isolated from Inner Mongol tradition
In spontaneous fermentation yoghurt, with excellent anti-gastrointestinal tract digestive juice tolerance, it can survive in animal intestinal tract；The bacterial strain
China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved in, the preservation time is on 06 28th, 2012,
Preserving number is CGMCC No.6312.
Lactobacillus casei (Lactobacillus casei) Zhang of the present invention, is separated from koumiss
The probiotics strain of acidproof and resistance to bile acid；The bacterial strain is preserved in Chinese microorganism strain preservation management on April 21st, 2006
Committee's General Microbiological Culture collection, preserving number CGMCC No.1697.
It is further preferred that the lactic acid bacteria composite fungicide of salt-soda soil peanut quality and yield traits is improved as described above, should
Lactic acid bacteria composite fungicide is by Bacillus bifidus (Bifidobacterium animalis subsp.Lactis) V9, the newborn bar of plant
Bacterium (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang presses 1：(2-
3)：The number of viable of (2-3) is than composition.
The present inventor has been surprisingly found that when screening suppresses the Antagonistic Fungi of saline and alkaline tibet milkwort root pathogen, contains Bacillus bifidus
(Bifidobacterium animalis subsp.Lactis) V9, Lactobacillus plantarum (Lactobacillus plantarum)
P-8 and Lactobacillus casei (Lactobacillus casei) Zhang composite bacteria liquid do not have obvious inhibiting effect to pathogen,
But there is obvious influence to the growth metabolism of peanut, the quality and yield character of peanut can be improved.Based on this discovery, the present invention
These three bacterium have been prepared into composite bacteria agent by people by being inoculated in cultivation and fermentation in MRS nutrient solutions.Therefore, the invention provides
The application of above-mentioned composite bacteria agent, i.e.,：Application of the above-mentioned lactic acid bacteria composite fungicide on salt-soda soil peanut quality is improved；Above-mentioned
Application of the lactic acid bacteria composite fungicide in salt-soda soil peanut yield character is improved.
Moreover, it relates to lactic acid bacteria composite fungicide can adopt and prepare with the following method：
(1) by the Bacillus bifidus (Bifidobacterium animalis subsp.Lactis) V9, the newborn bar of plant
Bacterium (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang presses 4%-
10% inoculum concentration is inoculated in MRS nutrient solutions respectively, incubated 12-24 hours under the conditions of 35-37 DEG C, through centrifuge washing
Afterwards, the cow's milk of sterilized non-fat containing 8%-12% liquid, 0.5%-0.6% glucose and 0.5%-0.8% are added on each bacterial sediment
The mixed liquor of yeast extract, and its bacterium number is adjusted more than 2.0 × 109Cfu/mL, pours into culture respectively after mixing；
(2) Bacillus bifidus (Bifidobacterium animalis subsp.Lactis) obtained step (1)
V9, Lactobacillus plantarum (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei)
Zhang strain cultures are inoculated in MRS nutrient solutions respectively, incubated 18-24 hours under the conditions of 35-37 DEG C, then connect respectively
Plant in the mixed liquor of the cow's milk of sterilized non-fat containing 8%-12% liquid, 0.5%-0.6% glucose and 0.5-0.8% yeast extracts,
Cultivated 18-24 hours under the conditions of 35-37 DEG C；
(3) each bacterium solution for obtaining step (2) is according to Lactobacillus plantarum (Lactobacillus plantarum) P-8：It is dry
Lactobacillus paracasei (Lactobacillus casei) Zhang：Lactobacillus plantarum (Lactobacillus plantarum)
CCFM8661 is 1：(2-3)：The ratio of (2-3) is mixed, and protective agent is added in mixed bacteria liquid, described protectant group
Turn into：90-120g/L skimmed milk powers, 25-35mL/L glycerine, 90-120g/L maltodextrins, 120-180g/L seas paint sugar and 8-
13g/L L-sodiums, adjustment viable count is more than 2 × 109Cfu/mL, pH value is 5-6.
Lactic acid bacteria composite fungicide of the present invention is configured to after bacterium solution to be applied to during peanut cultivation, usage amount and
Method：Apply for the first time, in the peanut seeding phase, consumption is that (total viable count content is more than 2 × 10 to 2 liters/mu9Cfu/mL), if adopting
Manually planting patterns, can be after trench digging, and clear water dilution is sprayed in ditch using agricultural atomizer；If being covered using peanut seeding
Film all-in-one, is placed in all-in-one explosive barrel after can directly bacterium solution clear water be diluted and uses, no matter which kind of method of application should be noted that bacterium
Liquid can not be used together with agricultural chemicals, and both use times should be spaced more than 2 days；Second of administration, in the peanut pod phase, consumption is
(total viable count content is more than 2 × 10 to 2 liters/mu9Cfu/mL), it is watered after dilution, sprays or pour in peanut root, conditionally
Area can carry out drip irrigation, water irrigating depth wetting soil 8-10cm using under-film drip irrigation mode.
Compared with prior art, the active bacteria in lactic acid bacteria composite fungicide of the present invention is by Bacillus bifidus
(Bifidobacterium animalis subsp.Lactis) V9, Lactobacillus plantarum (Lactobacillus plantarum)
P-8 and Lactobacillus casei (Lactobacillus casei) Zhang compositions, the lactic acid bacteria composite fungicide is to saline and alkaline tibet milkwort root
Growth metabolism has obvious influence, it is particularly possible to improve the quality and yield character of saline and alkaline tibet milkwort root, so as to be saline and alkaline tibet milkwort root
The improvement of quality and the raising of yield provide ideal bacterial manure raw material.
Embodiment
Form is described in further detail again to the above of the present invention by the following examples, but should not manage this
The scope solved as above-mentioned theme of the invention is only limitted to following embodiment, and all technologies realized based on the above of the present invention are equal
Belong to the scope of the present invention.In addition, the experimental method in following embodiments, is conventional method unless otherwise instructed.
Embodiment 1：The preparation of compound lactobacillus agent
(1) by Bacillus bifidus (Bifidobacterium animalis subsp.Lactis) V9, Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang presses 6% inoculation
Amount is inoculated in MRS nutrient solutions respectively, incubated 18 hours under the conditions of 35-37 DEG C, after centrifuge washing, on bacterial sediment
Add 10% sterilized non-fat cow's milk liquid, 0.6% glucose and 0.8% yeast extract, and adjust its bacterium number more than 2.0 ×
109Cfu/mL, pours into culture respectively after mixing.
(2) protective agent is prepared using sterilized water, constitutes and be：L00g/L skimmed milk powers, 30mL/L glycerine, l00g/L malt paste
Essence, 150g/L seas paint sugar and l0g/L L-sodiums.
(3) Bacillus bifidus (Bifidobacterium animalis subsp.Lactis) obtained step (1)
V9, Lactobacillus plantarum (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei)
Zhang strain cultures are inoculated in MRS nutrient solutions respectively, incubated 18 hours under the conditions of 35-37 DEG C, inoculate 10%
In the mixed liquor of sterilized non-fat cow's milk liquid, 0.6% glucose and 0.8% yeast extract, 20 are cultivated under the conditions of 35-37 DEG C small
When.
(4) each bacterium solution for obtaining step (3) is according to Bacillus bifidus (Bifidobacterium animalis
Subsp.Lactis) V9, Lactobacillus plantarum (Lactobacillus plantarum) P-8 and Lactobacillus casei
(Lactobacillus casei) Zhang is 2:2:1 ratio is mixed, and protective agent prepared by step (2) is added to mixing
In bacterium solution, its viable count is adjusted more than 2 × 109Cfu/mL, pH value is 5-6.
Embodiment 2：The preparation of compound lactobacillus agent
(1) by Lactobacillus plantarum (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus
Casei) Zhang is inoculated in MRS nutrient solutions respectively by 6% inoculum concentration, incubated 18 hours under the conditions of 35-37 DEG C, warp
After centrifuge washing, 10% sterilized non-fat cow's milk liquid, 0.6% glucose and 0.8% yeast extract are added on bacterial sediment, and adjust
Its whole bacterium number is more than 2.0 × 109Cfu/mL, pours into culture respectively after mixing.
(2) protective agent is prepared using sterilized water, constitutes and be：L00g/L skimmed milk powers, 30mL/L glycerine, l00g/L malt paste
Essence, 150g/L seas paint sugar and l0g/LL- sodium glutamates.
(3) Lactobacillus plantarum (Lactobacillus plantarum) P-8, the Lactobacillus casei obtained step (1)
(Lactobacillus casei) Zhang strain cultures are inoculated in MRS nutrient solutions respectively, and constant temperature is trained under the conditions of 35-37 DEG C
Support 18 hours, in the mixed liquor of sterilized non-fat cow's milk liquid, 0.6% glucose and 0.8% yeast extract for inoculating 10%,
Cultivated 20 hours under the conditions of 35-37 DEG C.
(4) each bacterium solution for obtaining step (3) is according to Lactobacillus plantarum (Lactobacillus plantarum) P-8：It is dry
Lactobacillus paracasei (Lactobacillus casei) Zhang is 1：1 ratio is mixed, and protective agent prepared by step (2) is added
Into mixed bacteria liquid, its viable count is adjusted more than 2 × 109Cfu/mL, pH value is 5-6.
Embodiment 3：The preparation of Bacillus bifidus microbial inoculum
(1) by Bacillus bifidus (Bifidobacterium animalis subsp.Lactis) V9 press 6% inoculum concentration
MRS nutrient solutions are inoculated in, incubated 18 hours under the conditions of 35-37 DEG C, after centrifuge washing, are added on bacterial sediment
10% sterilized non-fat cow's milk liquid, 0.6% glucose and 0.8% yeast extract, and its bacterium number is adjusted more than 2.0 × 109Cfu/mL,
Culture is poured into after mixing.
(2) protective agent is prepared using sterilized water, constitutes and be：L00g/L skimmed milk powers, 30mL/L glycerine, l00g/L malt paste
Essence, 150g/L seas paint sugar and l0g/LL- sodium glutamates.
(3) Bacillus bifidus (the Bifidobacterium animalis subsp.Lactis) V9 obtained step (1)
Strain culture is inoculated in MRS nutrient solutions respectively, incubated 18 hours under the conditions of 35-37 DEG C, inoculates 10% sterilizing
In the mixed liquor of skimmed milk liquid, 0.6% glucose and 0.8% yeast extract, cultivated 20 hours under the conditions of 35-37 DEG C.
(4) protective agent for preparing step (2) is added in the nutrient solution of step (3), adjust its viable count more than 2 ×
109Cfu/mL, pH value is 5-6.
Embodiment 4：Experiment of each microbial inoculum in the peanut cultivation of salt-soda soil
Test place：Dongying city Lijin County Ting Luo towns Mao Tuo villages
Experimental period：2016.05-2016.10
Test crop：Peanut (kind is small blue and white No. 9)
Test soil：(8.65) soil salt content 2.83g/kg, pH are to moderate saline-alkali soil
Test group：Compound bacteria A groups prepare microbial inoculum using embodiment 2, and Bacillus bifidus group applies embodiment 3 and prepares bacterium
Agent, compound bacteria B groups prepare microbial inoculum using embodiment 1.
Usage amount and method：Apply for the first time, in the peanut seeding phase, consumption is 2 liters/mu (microbial inoculum prepared by each embodiment
Total viable count content 3 × 10 in bacterium solution, each bacterium solution is configured to respectively9Cfu/mL or so), can be according to artificial growth mode
After trench digging, clear water dilution is sprayed in ditch using agricultural atomizer；If, can be directly by bacterium using peanut seeding film mulching integrated machine
Be placed in all-in-one explosive barrel and use after the dilution of liquid clear water, no matter which kind of method of application should be noted that bacterium solution and agricultural chemicals can not together with make
With both use times should be spaced more than 2 days；Second of administration, in the peanut pod phase, consumption is 2 liters/mu (each embodiment system
Standby microbial inoculum is configured to total viable count content 3 × 10 in bacterium solution, each bacterium solution respectively9Cfu/mL or so), it is watered after dilution, sprays
Or pour in peanut root, conditional region can carry out drip irrigation, water irrigating depth wetting soil 8-10cm using under-film drip irrigation mode
.
Cultivate administration：1 mu of experimental plot of each group, peanut is planted using overlay film start toughness, row spacing 90cm, per the row of ridge 2, on ridge
Small line-spacing 35cm, hill spacing 11cm (per 1, cave), every mu of 13474 cave (strain).Every mu is applied fertilizer specially for peanut, N-P-K content 45%
(10：18：17), 50 kilograms every mu, the later stage no longer topdresses.
Experimental result：
1st, to the influence of peanut stem index of correlation
After the compound lactobacillus microbial inoculum that the present invention is applied on saline and alkaline tibet milkwort root, the short strongization development of plant reduces overground part
The nutrient consumption of invalid growth, optimization plant nutrition is utilized, and improves the resistance of plant.Compound lactobacillus B groups are relative to sky
White control group, the effective branch amount of peanut improves 9.32%, and peanut stem rugosity improves 5.98%, and first pair of branch rugosity is improved
4.77%, stem fresh weight improves 16.23%, and dry weight improves 11.77% (table 1).Compared by statistics, in effective branch amount, stem
This three aspect of fresh weight and stem weight, B groups have significant difference (P ＜ 0.05) compared to A groups and Bacillus bifidus group, and this illustrates pair
Discrimination lactobacillus V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang can concertedness strengthen salt-soda soil plant resistance, improve
Utilization rate of the peanut to nutrient.
Respectively the lower peanut stem index of correlation of processing compares table 1
Note：A groups are Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs, and B groups are Bacillus bifidus V9+ Lactobacillus plantarums P-8
+ Lactobacillus casei Zhang.Compared with blank control group,▲P ＜ 0.05；B groups are compared with A groups,★P ＜ 0.05；B groups and bifid breast
Bacillus group compares,■P ＜ 0.05.
2nd, to the influence of peanut leaf index of correlation
Compound lactobacillus B groups are relative to blank control group, and peanut leaf area improves 8.50%, and leaf fresh weight improves 13.33%,
Leaf dry weight improves 19.60% (table 2).Compared by statistics, in terms of leaf fresh weight and dry weight, B groups are compared to A groups, the newborn bar of bifid
Bacterium group has significant difference (P ＜ 0.05), this explanation Bacillus bifidus V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang
Can concertedness improve saline and alkaline tibet milkwort root peanut leaf quantity and leaf area, be favorably improved the photosynthesis of peanut.
Respectively the lower peanut leaf index of correlation of processing compares table 2
Note：A groups are Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs, and B groups are Bacillus bifidus V9+ Lactobacillus plantarums P-8
+ Lactobacillus casei Zhang.Compared with blank control group,▲P ＜ 0.05；B groups are compared with A groups,★P ＜ 0.05；B groups and bifid breast
Bacillus group compares,■P ＜ 0.05.
3rd, to the influence of peanut pin index of correlation
After the compound lactobacillus microbial inoculum that the present invention is applied on saline and alkaline tibet milkwort root, the fruit pin quantity and son of peanut are significantly improved
Room handle weight.Compound lactobacillus B groups relative to blank control group, peanut bury fruit pin number number improve 29.04%, ovary handle fresh weight
76.05% is improved, ovary handle dry weight improves 42.79% (table 3).Compared by statistics, in the fruit pin number that buries, ovary handle fresh weight
With this three aspect of dry weight, B groups have significant difference (P ＜ 0.05) compared to A groups, Bacillus bifidus group, this newborn bar of explanation bifid
Bacterium V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang can concertedness improve peanut fruit pin quantity and ovary handle weight.
Respectively the lower peanut pin index of correlation of processing compares table 3
Note：A groups are Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs, and B groups are Bacillus bifidus V9+ Lactobacillus plantarums P-8
+ Lactobacillus casei Zhang.Compared with blank control group,▲P ＜ 0.05；B groups are compared with A groups,★P ＜ 0.05；B groups and bifid breast
Bacillus group compares,■P ＜ 0.05.
4th, to the influence of peanut yield index
After the compound lactobacillus microbial inoculum that the present invention is applied on saline and alkaline tibet milkwort root, the finger such as peanut pod quantity and weight is improved
Mark, improves the yield of peanut.Compound lactobacillus B groups are relative to blank control group, and peanut pod volume improves 14.04%, flower
Raw pod fresh weight improves 13.13%, and dry weight improves 6.98%, and peanut pod sum improves 30.64%, and it embezzles the raising of fruit number
16.0%, output increased 7.86% (table 4).Compared by statistics, pod volume$$$$, pod fresh weight, total fruit number, full fruit number this
Four aspects, B groups have significant difference (P ＜ 0.05) compared to A groups, Bacillus bifidus group, this explanation Bacillus bifidus V9, plant breast
Bacillus P-8 and Lactobacillus casei Zhang can concertedness improve the indexs such as peanut pod quantity and weight, improve the yield of peanut.
Respectively the lower peanut yield index of correlation of processing compares table 4
Note：A groups are Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs, and B groups are Bacillus bifidus V9+ Lactobacillus plantarums P-8
+ Lactobacillus casei Zhang.Compared with blank control group,▲P ＜ 0.05；B groups are compared with A groups,★P ＜ 0.05；B groups and bifid breast
Bacillus group compares,■P ＜ 0.05.
5th, lactic acid bacteria applies the influence to salt-soda soil peanut quality character
Compound lactobacillus B groups are relative to blank control group, and protein content improves 4.0%, and fat content improves 0.7%
(table 5).Compared by statistics, in terms of protein content, B groups have significant difference (P compared to A groups, Bacillus bifidus group
＜ 0.05), this explanation Bacillus bifidus V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang can concertedness improve peanut
Protein content in benevolence, so as to improve the quality of saline and alkaline tibet milkwort root.
Respectively the lower peanut quality index of correlation of processing compares table 5
Note：A groups are Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs, and B groups are Bacillus bifidus V9+ Lactobacillus plantarums P-8
+ Lactobacillus casei Zhang.Compared with blank control group,▲P ＜ 0.05；B groups are compared with A groups,★P ＜ 0.05；B groups and bifid breast
Bacillus group compares,■P ＜ 0.05.
Conclusion is with discussing：
On saline and alkaline tibet milkwort root after the compound lactobacillus microbial inoculum of the administrations present invention, the germination percentage of peanut is improved, is reduced
The growth of ground non productive tiller, improves leaf area and quantity, improves the quantity and weight of underground part pod, improve peanut
Economic characters and yield.Consider from quality perspective, compound lactobacillus microbial inoculum of the invention improve in peanut nutritive value compared with
High protein content, influences not notable to fat content, has certain positive role for the quality for improving saline and alkaline tibet milkwort root.
Claims (9)
1. the lactic acid bacteria composite fungicide of a kind of raising salt-soda soil peanut quality and yield traits, it is characterised in that the lactic acid bacteria is answered
The active bacteria in microbial inoculum is closed by Bacillus bifidus V9 (Bifidobacterium animalis subsp.Lactis V9), plant
Lactobacillus (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang compositions.
2. improving the lactic acid bacteria composite fungicide of salt-soda soil peanut quality and yield traits according to claim 1, its feature exists
In the lactic acid bacteria composite fungicide is by Bacillus bifidus (Bifidobacterium animalis subsp.Lactis) V9, plant
Lactobacillus (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang presses 1：
(2-3)：The number of viable of (2-3) is than composition.
3. application of the lactic acid bacteria composite fungicide on salt-soda soil peanut quality is improved described in claim 1 or 2.
4. application of the lactic acid bacteria composite fungicide in salt-soda soil peanut yield character is improved described in claim 1 or 2.
5. the preparation method of lactic acid bacteria composite fungicide according to claim 1 or 2, it is characterised in that this method is included such as
Lower step：
(1) by the Bacillus bifidus (Bifidobacterium animalis subsp.Lactis) V9, Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang presses 4%-10%
Inoculum concentration be inoculated in MRS nutrient solutions respectively, it is incubated 12-24 hours under the conditions of 35-37 DEG C, after centrifuge washing,
The cow's milk of sterilized non-fat containing 8%-12% liquid, 0.5%~0.6% glucose and 0.5%~0.8% yeast are added on each bacterial sediment
The mixed liquor of medicinal extract, and its bacterium number is adjusted more than 2.0 × 109Cfu/mL, pours into culture respectively after mixing；
(2) Bacillus bifidus (Bifidobacterium animalis subsp.Lactis) V9 for obtaining step (1), plant
Thing lactobacillus (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang bacterium
Strain culture is inoculated in MRS nutrient solutions respectively, incubated 18-24 hours under the conditions of 35-37 DEG C, then is inoculated in contains respectively
In the mixed liquor of 8%-12% sterilized non-fat cow's milk liquid, 0.5%-0.6% glucose and 0.5~0.8% yeast extract, in 35-
Cultivated 18-24 hours under the conditions of 37 DEG C；
(3) each bacterium solution for obtaining step (2) is according to Lactobacillus plantarum (Lactobacillus plantarum) P-8：Cheese breast
Bacillus (Lactobacillus casei) Zhang：Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 is
1：(2-3)：The ratio of (2-3) is mixed, and protective agent is added in mixed bacteria liquid, and described protectant composition is：90-
120g/L skimmed milk powers, 25-35mL/L glycerine, 90-120g/L maltodextrins, 120-180g/L seas paint sugar and 8-13g/L L- paddy
Propylhomoserin sodium, adjustment viable count is more than 2 × 109Cfu/mL, pH value is 5-6.
6. the peanut cultivation method of a kind of raising salt-soda soil peanut quality and yield traits, it is characterised in that this method includes applying
The bacterium solution prepared with the lactic acid bacteria composite fungicide described in claim 1 or 2, is applied to the peanut seeding phase, consumption is 2-3 for the first time
Liter/mu, total viable count is more than 2 × 109cfu/mL；The peanut pod phase is applied to for the second time, and consumption is 2-3 liters/mu, total viable count
More than 2 × 109Cfu/mL, is watered after dilution, sprays or pours or drip irrigation is in peanut root.
7. the peanut cultivation method of salt-soda soil peanut quality and yield traits is improved according to claim 6, it is characterised in that
According to artificial growth mode, add clear water to dilute the bacterium solution after trench digging, sprayed using agricultural atomizer in ditch.
8. the peanut cultivation method of salt-soda soil peanut quality and yield traits is improved according to claim 6, it is characterised in that
According to peanut seeding film mulching integrated machine, then directly add clear water to be placed in after diluting in all-in-one explosive barrel the bacterium solution and use.
9. according to the peanut cultivation method of any one of the claim 6-8 raising salt-soda soil peanut qualities and yield traits, its
It is characterised by, described bacterium solution can not be used together with agricultural chemicals, both use times should be spaced more than 2 days.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110106127A (en) * | 2019-06-18 | 2019-08-09 | 青岛农业大学 | The application and breeding method of a kind of composite bacteria agent in Chinese pistache seedling fostering |
| CN112500220A (en) * | 2020-11-05 | 2021-03-16 | 东北农业大学 | Liquid compound microorganism foliar fertilizer for improving saline-alkali soil and application thereof |
| CN112592222A (en) * | 2020-11-05 | 2021-04-02 | 东北农业大学 | Solid-state composite microorganism rooting agent for improving saline-alkali soil and application thereof |
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| CN1342170A (en) * | 1999-02-05 | 2002-03-27 | 维特罗莱夫英国有限公司 | Process for cross-linking hyaluronic acid to polymers |
| CN106282052A (en) * | 2016-08-08 | 2017-01-04 | 青岛农业大学 | A kind of compound lactobacillus preparation improveing coastal saline soil and application thereof |
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| CN1342170A (en) * | 1999-02-05 | 2002-03-27 | 维特罗莱夫英国有限公司 | Process for cross-linking hyaluronic acid to polymers |
| CN106282052A (en) * | 2016-08-08 | 2017-01-04 | 青岛农业大学 | A kind of compound lactobacillus preparation improveing coastal saline soil and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110106127A (en) * | 2019-06-18 | 2019-08-09 | 青岛农业大学 | The application and breeding method of a kind of composite bacteria agent in Chinese pistache seedling fostering |
| CN112500220A (en) * | 2020-11-05 | 2021-03-16 | 东北农业大学 | Liquid compound microorganism foliar fertilizer for improving saline-alkali soil and application thereof |
| CN112592222A (en) * | 2020-11-05 | 2021-04-02 | 东北农业大学 | Solid-state composite microorganism rooting agent for improving saline-alkali soil and application thereof |
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