BR9813110A - Sequências de ácido nucléico de msp-1 modificada e métodos para o aumento de nìveis de mrna e de expressão de proteìnas em sistemas de células - Google Patents

Sequências de ácido nucléico de msp-1 modificada e métodos para o aumento de nìveis de mrna e de expressão de proteìnas em sistemas de células

Info

Publication number
BR9813110A
BR9813110A BR9813110-9A BR9813110A BR9813110A BR 9813110 A BR9813110 A BR 9813110A BR 9813110 A BR9813110 A BR 9813110A BR 9813110 A BR9813110 A BR 9813110A
Authority
BR
Brazil
Prior art keywords
msp
nucleic acid
acid sequences
methods
protein
Prior art date
Application number
BR9813110-9A
Other languages
English (en)
Inventor
Li How Chen
Harry Meade
Original Assignee
Genzyme Transgenics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Transgenics Corp filed Critical Genzyme Transgenics Corp
Publication of BR9813110A publication Critical patent/BR9813110A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C07K14/445—Plasmodium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4732—Casein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Patente de Invenção: <B>"SEQuêNCIAS DE áCIDO NUCLéICO DE MSP-1 MODIFICADA E MéTODOS PARA O AUMENTO DE NìVEIS DE mRNA E DE EXPRESSãO DE PROTEìNAS EM SISTEMAS DE CéLULAS"<D>. A invenção proporciona seq³ências de ácido nucléico recombinante modificadas e a expressão de proteína da proteína malárica de superfície MSP-1 que é conhecida como sendo difícil de ser expressa em sistemas de culturas de células, sistemas de culturas de células de mamíferos ou em animais transgênicos. A proteína candidata de preferência para a expressão com a utilização da técnica recombinante da invenção é a proteína MSP-1 das seq³ências de codificação de DNA que compreende um teor reduzido de At em regiões ricas em AT e/ou os motivos de instabilidade do mRNA e/ou códons raros com relação aos do gene da MSP-1 nativa.
BR9813110-9A 1997-10-20 1998-10-20 Sequências de ácido nucléico de msp-1 modificada e métodos para o aumento de nìveis de mrna e de expressão de proteìnas em sistemas de células BR9813110A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6259297P 1997-10-20 1997-10-20
US8564998P 1998-05-15 1998-05-15
PCT/US1998/022226 WO1999020774A2 (en) 1997-10-20 1998-10-20 NOVEL MODIFIED MSP-1 NUCLEIC ACID SEQUENCES AND METHODS FOR INCREASING mRNA LEVELS AND PROTEIN EXPRESSION IN CELL SYSTEMS

Publications (1)

Publication Number Publication Date
BR9813110A true BR9813110A (pt) 2000-08-15

Family

ID=26742460

Family Applications (2)

Application Number Title Priority Date Filing Date
BR9812945-7A BR9812945A (pt) 1997-10-20 1998-10-20 Sequências de ácido nucléico modificadas e processos para aumentar os nìveis de mrna e expressão de sistemas celulares
BR9813110-9A BR9813110A (pt) 1997-10-20 1998-10-20 Sequências de ácido nucléico de msp-1 modificada e métodos para o aumento de nìveis de mrna e de expressão de proteìnas em sistemas de células

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR9812945-7A BR9812945A (pt) 1997-10-20 1998-10-20 Sequências de ácido nucléico modificadas e processos para aumentar os nìveis de mrna e expressão de sistemas celulares

Country Status (11)

Country Link
US (5) US7632980B1 (pt)
EP (3) EP1555319A3 (pt)
JP (2) JP2001520048A (pt)
KR (2) KR20010031210A (pt)
CN (2) CN1325646C (pt)
AP (2) AP1678A (pt)
AU (2) AU760231B2 (pt)
BR (2) BR9812945A (pt)
CA (2) CA2306799A1 (pt)
OA (2) OA11520A (pt)
WO (2) WO1999020766A2 (pt)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001520048A (ja) 1997-10-20 2001-10-30 ジェンザイム トランスジェニックス コーポレイション 新規な改変されたＭＳＰ−１核酸配列並びに細胞システムにおいてｍＲＮＡレベル及び蛋白質発現を増大させる方法
US7022830B2 (en) * 2000-08-17 2006-04-04 Tripep Ab Hepatitis C virus codon optimized non-structural NS3/4A fusion gene
CN1176945C (zh) * 2001-02-01 2004-11-24 中国人民解放军第二军医大学 疟原虫融合抗原及其制法和用途
ATE291925T1 (de) * 2001-06-05 2005-04-15 Curevac Gmbh Stabilisierte mrna mit erhöhtem g/c-gehalt und optimierter codon usage für die gentherapie
DE10162480A1 (de) 2001-12-19 2003-08-07 Ingmar Hoerr Die Applikation von mRNA für den Einsatz als Therapeutikum gegen Tumorerkrankungen
WO2003059381A2 (en) * 2002-01-18 2003-07-24 Curevac Gmbh Immunogenic preparations and vaccines on the basis of mrna
EP1490494A1 (en) * 2002-04-01 2004-12-29 Walter Reed Army Institute of Research Method of designing synthetic nucleic acid sequences for optimal protein expression in a host cell
WO2003084472A2 (en) * 2002-04-01 2003-10-16 Walter Reed Army Institute Of Research Recombinant p. falciparum merozoite protein-142 vaccine
US20050197496A1 (en) * 2004-03-04 2005-09-08 Gtc Biotherapeutics, Inc. Methods of protein fractionation using high performance tangential flow filtration
US20060130159A1 (en) * 2004-12-09 2006-06-15 Nick Masiello Method of purifying recombinant MSP 1-42 derived from Plasmodium falciparum
GB0510763D0 (en) * 2005-05-27 2005-06-29 London School Hygiene & Tropical Medicine Malaria vaccines
US20080213318A1 (en) * 2005-07-05 2008-09-04 Hawaii Biotech, Inc. Malaria MSP-1 C-terminal enhanced subunit vaccine
US7531632B2 (en) * 2006-02-16 2009-05-12 Gtc Biotherapeutics, Inc. Clarification of transgenic milk using depth filtration
JP2009106163A (ja) * 2007-10-26 2009-05-21 Kyushu Univ 核酸配列、ベクター、形質転換体、製造方法、及び、核酸配列プライマー
EP2141177A1 (en) * 2008-07-04 2010-01-06 Bujard, Hermann MSP-1 protein preparations from Plasmodium
GB0909689D0 (en) * 2009-06-05 2009-07-22 Trinity College Dublin Fluorescent proteins
WO2012019168A2 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
EP2622064B1 (en) 2010-10-01 2019-05-29 Modernatx, Inc. Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
CN116585504A (zh) 2010-12-30 2023-08-15 法国化学与生物科技实验室 作为病原体灭活剂的二元醇
US8710200B2 (en) 2011-03-31 2014-04-29 Moderna Therapeutics, Inc. Engineered nucleic acids encoding a modified erythropoietin and their expression
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
HRP20220250T1 (hr) 2011-10-03 2022-04-29 Modernatx, Inc. Modificirani nukleozidi, nukleotidi i nukleinske kiseline, te njihove uporabe
EP2791160B1 (en) 2011-12-16 2022-03-02 ModernaTX, Inc. Modified mrna compositions
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9878056B2 (en) 2012-04-02 2018-01-30 Modernatx, Inc. Modified polynucleotides for the production of cosmetic proteins and peptides
EP2834259A4 (en) 2012-04-02 2016-08-24 Moderna Therapeutics Inc MODIFIED POLYNUCLEOTIDES
ES2921623T3 (es) 2012-11-26 2022-08-30 Modernatx Inc ARN modificado terminalmente
MX2015010427A (es) 2013-02-13 2016-03-17 Lab Francais Du Fractionnement Anticuerpos anti-tnf-alfa altamente galactosilados y sus usos.
US10034921B2 (en) 2013-02-13 2018-07-31 Laboratoire Français Du Fractionnement Et Des Biotechnologies Proteins with modified glycosylation and methods of production thereof
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
JP2016532100A (ja) 2013-07-05 2016-10-13 ラボラトワール・フランセ・デュ・フラクシオンマン・エ・デ・ビョテクノロジーＬａｂｏｒａｔｏｉｒｅ Ｆｒａｎｃａｉｓ Ｄｕ Ｆｒａｃｔｉｏｎｎｅｍｅｎｔ Ｅｔ Ｄｅｓ Ｂｉｏｔｅｃｈｎｏｌｏｇｉｅｓ アフィニティークロマトグラフィーマトリックス
EP3052106A4 (en) 2013-09-30 2017-07-19 ModernaTX, Inc. Polynucleotides encoding immune modulating polypeptides
EP3052521A1 (en) 2013-10-03 2016-08-10 Moderna Therapeutics, Inc. Polynucleotides encoding low density lipoprotein receptor
CA2925021A1 (en) 2013-11-01 2015-05-07 Curevac Ag Modified rna with decreased immunostimulatory properties

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3751873T2 (de) 1986-04-09 1997-02-13 Genzyme Corp Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern
EP0832981A1 (en) * 1987-02-17 1998-04-01 Pharming B.V. DNA sequences to target proteins to the mammary gland for efficient secretion
US4873316A (en) * 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5225534A (en) 1987-09-08 1993-07-06 Hoffmann-La Roche Inc. Recombinant malarial polypeptides
US5194587A (en) 1987-12-30 1993-03-16 Behringwerke Aktiengesellschaft Malaria-specific DNA sequences, expression products thereof, and the use thereof
NZ230375A (en) 1988-09-09 1991-07-26 Lubrizol Genetics Inc Synthetic gene encoding b. thuringiensis insecticidal protein
US5231168A (en) * 1988-09-16 1993-07-27 Statens Seruminstitut Malaria antigen
GB8905857D0 (en) 1989-03-14 1989-04-26 Hoffmann La Roche Plasmodium merozoite rhoptries antigen
US5646247A (en) 1989-04-05 1997-07-08 New York University Merozoite antigens localized at the apical end of the parasite
ATE140027T1 (de) 1989-12-01 1996-07-15 Pharming Bv Herstellung rekombinanter polypeptide durch rinder und transgene methoden
GB9012580D0 (en) 1990-06-06 1990-07-25 Univ Nijmegen Novel protein
DE4041836A1 (de) 1990-12-24 1992-06-25 Behringwerke Ag Protektive plasmodium falciparum hybridproteine, die teilsequenzen der malaria-antigene hrpii und serp enthalten, ihre herstellung und verwendung
US5831141A (en) 1991-01-11 1998-11-03 United States Of America As Represented By The Department Of Health And Human Services Expression of a heterologous polypeptide in mammary tissue of transgenic non-human mammals using a long whey acidic protein promoter
US5766597A (en) * 1991-03-07 1998-06-16 Virogenetics Corporation Malaria recombinant poxviruses
AU663101B2 (en) * 1991-08-13 1995-09-28 Wisconsin Milk Marketing Board DNA sequence encoding bovine alpha-lactalbumin and methods of use
US5643578A (en) * 1992-03-23 1997-07-01 University Of Massachusetts Medical Center Immunization by inoculation of DNA transcription unit
US5856178A (en) * 1993-08-30 1999-01-05 Utah State University DNA cassettes for expression of lytic peptides in mammalian cells and transgenic organisms containing same
GB9318207D0 (en) * 1993-09-02 1993-10-20 Sandoz Ltd Improvements in or relating to organic compounds
US5827690A (en) 1993-12-20 1998-10-27 Genzyme Transgenics Corporatiion Transgenic production of antibodies in milk
US6713662B1 (en) 1994-07-27 2004-03-30 Pharming Intellectual Property B.V. Production of collagen in the milk of transgenic mammals
AUPM772494A0 (en) 1994-08-30 1994-09-22 Austin Research Institute, The Improvements in production of proteins in host cells
US5795737A (en) * 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
WO1997026911A1 (en) 1996-01-29 1997-07-31 Georgetown University Malaria vaccine based upon the addition of a msa1 peptide
FR2744723A1 (fr) 1996-02-14 1997-08-14 Pasteur Institut Proteine recombinante contenant un fragment c-terminal d'une proteine msp-1 d'un plasmodium infectieux pour l'homme pour la production de vaccins anti-paludiques
FR2744724B1 (fr) 1996-02-14 2002-08-02 Pasteur Institut Proteine recombinante contenant un fragment c-terminal de la proteine msp-1 d'un plasmodium infectieux pour l'homme pour la production de vaccins anti-paludiques
HRP970092A2 (en) * 1996-02-22 1998-04-30 Merck & Co Inc Synthetic hiv genes
US6268487B1 (en) 1996-05-13 2001-07-31 Genzyme Transgenics Corporation Purification of biologically active peptides from milk
US5956178A (en) * 1996-09-02 1999-09-21 Nikon Corporation Keplerian variable magnification viewfinder
US6114148C1 (en) 1996-09-20 2012-05-01 Gen Hospital Corp High level expression of proteins
DE19640817A1 (de) * 1996-10-02 1998-05-14 Hermann Prof Dr Bujard Rekombinantes Herstellungsverfahren für ein vollständiges Malaria-Antigen gp190/MSP 1
JP2001520048A (ja) 1997-10-20 2001-10-30 ジェンザイム トランスジェニックス コーポレイション 新規な改変されたＭＳＰ−１核酸配列並びに細胞システムにおいてｍＲＮＡレベル及び蛋白質発現を増大させる方法

Also Published As

Publication number Publication date
WO1999020774A3 (en) 1999-08-26
WO1999020766A2 (en) 1999-04-29
WO1999020774A2 (en) 1999-04-29
JP2001520048A (ja) 2001-10-30
CN1179048C (zh) 2004-12-08
AP1678A (en) 2006-11-27
JP2001520041A (ja) 2001-10-30
KR20010031208A (ko) 2001-04-16
US7632980B1 (en) 2009-12-15
OA11372A (en) 2004-01-27
EP1555319A3 (en) 2006-03-15
EP1025233A2 (en) 2000-08-09
CA2306799A1 (en) 1999-04-29
US7501553B2 (en) 2009-03-10
AP1219A (en) 2003-10-22
WO1999020766A3 (en) 1999-09-02
CA2306796A1 (en) 1999-04-29
AU760231B2 (en) 2003-05-08
US20050235371A1 (en) 2005-10-20
EP1555319A2 (en) 2005-07-20
OA11520A (en) 2004-02-09
AP2000001813A0 (en) 2000-06-30
US7354594B2 (en) 2008-04-08
AU1108799A (en) 1999-05-10
US20020144299A1 (en) 2002-10-03
BR9812945A (pt) 2000-08-08
US20050071890A1 (en) 2005-03-31
AU1108899A (en) 1999-05-10
US6593463B1 (en) 2003-07-15
CN1276834A (zh) 2000-12-13
KR20010031210A (ko) 2001-04-16
CN1276831A (zh) 2000-12-13
EP1025244A2 (en) 2000-08-09
AP2000001812A0 (en) 2000-06-30
CN1325646C (zh) 2007-07-11

Similar Documents

Publication Publication Date Title
BR9813110A (pt) Sequências de ácido nucléico de msp-1 modificada e métodos para o aumento de nìveis de mrna e de expressão de proteìnas em sistemas de células
Barnier et al. Mouse 89 kD heat shock protein: two polypeptides with distinct developmental regulation
BR0010725A (pt) Expressão e exportação de proteìnas de interferon-alfa como proteìnas de fusão de fc
ATE318896T1 (de) Rekombinante rna-replikase von hepatitis-c-virus
DK0941318T3 (da) Håndvægtformede konstruerede ekspressionssystemer til genterapi
ATE466948T1 (de) Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle
DE69131321D1 (de) Mutationen in der 5&#39;-3&#39;-exonukleaseaktivität thermostabiler dna-polymerasen
ATE90966T1 (de) Expression von protein-c.
PT941352E (pt) Milhoramentos em, ou relacionados com, o conteudo de amido em plantas.
TR200401186T4 (tr) Rekombinant bir RNA-virüs oluşumunu yönlendirebilen nükleik asit sekansları içerikli suni kromozom yapıları.
NO862601L (no) Ekspresjon av protein c.
KR960010863A (ko) 재조합 머사시딘 및 이의 제조방법
BR0113681A (pt) Polipeptìdeo tendo atividade da enzima lipoxigenase, polinucleotìdeo, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir uma lipoxigenase, sonda de oligonucleotìdeo, método para obter um polipeptìdeo com atividade de lipoxigenase, composição de massa, métodos para preparar uma massa ou um produto assado produzido da massa, e para oxigenar um substrato, e, composição detergente
BR9811865A (pt) Isolamento de um novo gene de fator de senescência, p23
DK1045901T3 (da) Ribozymale nucleinsyrer, der spalter CCR5 eller CXCR4
Argenton et al. The transcriptional regulation of the growth hormone gene is conserved in vertebrate evolution
Allen et al. Production of epidermal growth factor in Escherichia coli from a synthetic gene
ATE371022T1 (de) Fy7 polymerase
DE69838644D1 (de) Verfahren zur herstellung eines rekombinanten rinderadenovirus-vektors
KR960023056A (ko) 푸자리움 옥시스포룸으로부터의 페니실린 v 아미도하이드롤라아제 유전자
Greenblatt et al. Properties of the N gene transcription antitermination protein of bacteriophage lambda.
Adham et al. The structures of the bovine and porcine proacrosin genes and their conservation among mammals
Sakurai et al. Subunit composition of RNA polymerase II from the fission yeast Schizosaccharomyces pombe
BR9909388A (pt) Uso de pelo menos uma leishmania cisteìna proteinase, vacina, proteìna cpa do l. infantum, genes cpa e cpb do l. infantum, cpa ou cpb, e, uso das proteìnas cpa e/ou cpb ou das sequências de ácido nucléico do cpa e/ou cpb
BR9508430A (pt) Sequência nucleotídica ADN recombinante vetor de expressão com replicação autônoma e/ou integrativo célula recombinante polipeptideo processo de produção de um polipeptideo topoismorase isolada e utilização de uma topoisomeras IV

Legal Events

Date Code Title Description
TC Change of name
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 25 DA LPI.

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.