WO2011159092A3 - 신규 토양 미생물과 상기 토양 미생물로부터 분리된 신규한 산화환원효소, 상기 산화환원 효소를 암호화하는 유전자 및 이들을 이용한 비당체의 생산방법 - Google Patents

신규 토양 미생물과 상기 토양 미생물로부터 분리된 신규한 산화환원효소, 상기 산화환원 효소를 암호화하는 유전자 및 이들을 이용한 비당체의 생산방법 Download PDF

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WO2011159092A3
WO2011159092A3 PCT/KR2011/004361 KR2011004361W WO2011159092A3 WO 2011159092 A3 WO2011159092 A3 WO 2011159092A3 KR 2011004361 W KR2011004361 W KR 2011004361W WO 2011159092 A3 WO2011159092 A3 WO 2011159092A3
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Prior art keywords
oxidoreductase
novel
microorganism
aglycones
gene
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PCT/KR2011/004361
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English (en)
French (fr)
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WO2011159092A2 (ko
WO2011159092A9 (ko
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박준성
김덕희
김한곤
김은미
김병기
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(주)아모레퍼시픽
서울대학교산학협력단
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Priority claimed from KR1020100055988A external-priority patent/KR101749680B1/ko
Priority claimed from KR1020100066307A external-priority patent/KR101749681B1/ko
Application filed by (주)아모레퍼시픽, 서울대학교산학협력단 filed Critical (주)아모레퍼시픽
Priority to US13/704,210 priority Critical patent/US20130084601A1/en
Priority to CN201180038765.9A priority patent/CN103237884B/zh
Priority to JP2013515263A priority patent/JP5878524B2/ja
Publication of WO2011159092A2 publication Critical patent/WO2011159092A2/ko
Publication of WO2011159092A9 publication Critical patent/WO2011159092A9/ko
Publication of WO2011159092A3 publication Critical patent/WO2011159092A3/ko
Priority to HK13112245.3A priority patent/HK1184823A1/xx
Priority to US14/588,790 priority patent/US9394562B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/41—Rhizobium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 신규 리조비움 sp. GIN611(Rhizobiumsp.GIN611)KCTC11708BP또는 그의 세포 추출물, 당 분해 활성을 보이는 신규 산화환원 효소, 이를 암호화하는 유전자 및 재조합 벡터 단백질, 또는 재조합 단백질을 암호화하는 발현벡터를 포함하는 재조합 균주 등을 제공하고, 이들을 생촉매로 사용하여 천연물을 당 분해하는 방법을 제공한다. 또한, 이들을 이용하여 다양한 천연물의 비당체를 생산하는 방법을 제공한다. 본 발명의 신규 미생물에서 분리한 신규한 효소는 글루코시데이즈(glucosidase)군에 속하지 않고 산화환원효소 군에 속하는 효소로서 천연물의 당 분해 활성을 갖는다. 이 신규한 산화환원효소는 천연물 배당체에서 당을 산화시킴으로서 다양한 비당체를 생산할 수 있게 한다.
PCT/KR2011/004361 2010-06-14 2011-06-14 신규 토양 미생물과 상기 토양 미생물로부터 분리된 신규한 산화환원효소, 상기 산화환원 효소를 암호화하는 유전자 및 이들을 이용한 비당체의 생산방법 WO2011159092A2 (ko)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US13/704,210 US20130084601A1 (en) 2010-06-14 2011-06-14 Novel soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene
CN201180038765.9A CN103237884B (zh) 2010-06-14 2011-06-14 新的土壤微生物、从所述土壤微生物分离的新氧化还原酶、编码所述氧化还原酶的基因和使用所述微生物、氧化还原酶和基因产生糖苷配基的方法
JP2013515263A JP5878524B2 (ja) 2010-06-14 2011-06-14 新規土壌微生物、前記土壌微生物から分離された新規な酸化還元酵素、前記酸化還元酵素をコード化する遺伝子、及びこれらを利用した無糖体の生産方法
HK13112245.3A HK1184823A1 (en) 2010-06-14 2013-10-30 Novel soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene
US14/588,790 US9394562B2 (en) 2010-06-14 2015-01-02 Soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR1020100055988A KR101749680B1 (ko) 2010-06-14 2010-06-14 신규 토양 미생물과 상기 토양 미생물로부터 분리된 신규한 산화환원효소, 상기 산화환원 효소를 암호화하는 유전자 및 이들을 이용한 다양한 진세노사이드의 생산방법
KR10-2010-0055988 2010-06-14
KR10-2010-0066307 2010-07-09
KR1020100066307A KR101749681B1 (ko) 2010-07-09 2010-07-09 신규 토양 미생물과 상기 토양 미생물로부터 분리된 신규한 산화환원효소, 상기 산화환원 효소를 암호화하는 유전자 및 이들을 이용한 플라보노이드 비당체의 생산방법.

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/704,210 A-371-Of-International US20130084601A1 (en) 2010-06-14 2011-06-14 Novel soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene
US14/588,790 Continuation US9394562B2 (en) 2010-06-14 2015-01-02 Soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene

Publications (3)

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WO2011159092A2 WO2011159092A2 (ko) 2011-12-22
WO2011159092A9 WO2011159092A9 (ko) 2012-03-01
WO2011159092A3 true WO2011159092A3 (ko) 2012-05-18

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PCT/KR2011/004361 WO2011159092A2 (ko) 2010-06-14 2011-06-14 신규 토양 미생물과 상기 토양 미생물로부터 분리된 신규한 산화환원효소, 상기 산화환원 효소를 암호화하는 유전자 및 이들을 이용한 비당체의 생산방법

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US (2) US20130084601A1 (ko)
JP (1) JP5878524B2 (ko)
CN (1) CN103237884B (ko)
HK (1) HK1184823A1 (ko)
WO (1) WO2011159092A2 (ko)

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CN103342726B (zh) * 2013-07-16 2016-06-29 青龙高科技股份有限公司 一种降血糖油茶黄酮的制备方法及其应用
CN108486196A (zh) * 2015-05-20 2018-09-04 佛山市金骏康健康科技有限公司 一种淫羊藿次苷i或淫羊藿次苷c的制备方法
US10244245B2 (en) 2015-06-08 2019-03-26 Qualcomm Incorporated Content-adaptive application of fixed transfer function to high dynamic range (HDR) and/or wide color gamut (WCG) video data
KR101785809B1 (ko) * 2015-12-18 2017-10-18 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 진세노사이드 글리코시다제를 이용한 마이너 진세노사이드의 제조방법
WO2018201050A1 (en) * 2017-04-28 2018-11-01 Board Of Trustees Of Michigan State University Tryptathionine biosynthesis by flavin mono-oxygenase 1 (fmo1)
WO2023057235A1 (en) 2021-10-06 2023-04-13 Glycoscience, S.L. Process for preparing 3'-ketoglycoside compounds

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Also Published As

Publication number Publication date
US9394562B2 (en) 2016-07-19
US20130084601A1 (en) 2013-04-04
CN103237884B (zh) 2015-08-19
JP5878524B2 (ja) 2016-03-08
CN103237884A (zh) 2013-08-07
HK1184823A1 (en) 2014-01-30
JP2013534416A (ja) 2013-09-05
WO2011159092A2 (ko) 2011-12-22
WO2011159092A9 (ko) 2012-03-01
US20150184216A1 (en) 2015-07-02

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