IN2012DN03823A - - Google Patents

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Publication number
IN2012DN03823A
IN2012DN03823A IN3823DEN2012A IN2012DN03823A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A IN 3823DEN2012 A IN3823DEN2012 A IN 3823DEN2012A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A
Authority
IN
India
Prior art keywords
modified
vacuolar sorting
vps10
sorting activity
host cell
Prior art date
Application number
Other languages
English (en)
Inventor
Michael Meehl
Heping Lin
Byung-Kwon Choi
Original Assignee
Merck Sharp & Dohme
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Sharp & Dohme filed Critical Merck Sharp & Dohme
Publication of IN2012DN03823A publication Critical patent/IN2012DN03823A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
IN3823DEN2012 2009-10-30 2010-10-25 IN2012DN03823A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25637909P 2009-10-30 2009-10-30
US35066810P 2010-06-02 2010-06-02
PCT/US2010/053903 WO2011053541A1 (en) 2009-10-30 2010-10-25 Methods for the production of recombinant proteins with improved secretion efficiencies

Publications (1)

Publication Number Publication Date
IN2012DN03823A true IN2012DN03823A (ko) 2015-08-28

Family

ID=43922476

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3823DEN2012 IN2012DN03823A (ko) 2009-10-30 2010-10-25

Country Status (12)

Country Link
US (1) US20130011875A1 (ko)
EP (1) EP2494053A4 (ko)
JP (1) JP2013509180A (ko)
KR (1) KR20140015137A (ko)
CN (1) CN102686731A (ko)
AU (1) AU2010313608A1 (ko)
BR (1) BR112012009886A2 (ko)
CA (1) CA2777487A1 (ko)
IN (1) IN2012DN03823A (ko)
MX (1) MX2012004993A (ko)
RU (1) RU2012122166A (ko)
WO (1) WO2011053541A1 (ko)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011053545A1 (en) * 2009-10-30 2011-05-05 Merck Sharp & Dohme Corp. Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris
US9102969B2 (en) * 2011-03-11 2015-08-11 Kaneka Corporation Method for producing heterologous protein using yeast with disruption of VPS gene
JP6245528B2 (ja) 2012-01-05 2017-12-13 グリコス・フィンランド・オーワイＧｌｙｋｏｓ Ｆｉｎｌａｎｄ Ｏｙ プロテアーゼ欠損糸状菌細胞及びその使用方法
CN102559740B (zh) * 2012-02-25 2013-03-27 山东大学 一种提高酿酒酵母分泌表达异源蛋白的方法及专用酿酒酵母菌株
WO2013174927A1 (en) 2012-05-23 2013-11-28 Novartis International Pharmaceutical Limited Production of fucosylated glycoproteins
US20160017021A1 (en) * 2013-03-06 2016-01-21 Protalix Ltd. TNF alpha INHIBITOR POLYPEPTIDES, POLYNUCLEOTIDES ENCODING SAME, CELLS EXPRESSING SAME AND METHODS OF PRODUCING SAME
WO2015004241A2 (en) 2013-07-10 2015-01-15 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
EP3221455B1 (en) * 2014-11-20 2020-08-12 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
WO2018132821A2 (en) * 2017-01-13 2018-07-19 Bolt Threads, Inc. Elastomeric proteins
ES2962560T3 (es) 2017-07-31 2024-03-19 Melt&Marble Ab Célula fúngica con capacidad mejorada de producción de proteínas
SE2051317A1 (en) * 2020-11-11 2022-05-12 Nielsen Dina Petranovic A genetically modified yeast cell
CN113604373A (zh) * 2021-02-08 2021-11-05 江南大学 一种提高人源溶菌酶产量及酶活的毕赤酵母缺陷型菌株
CN113736818A (zh) * 2021-10-08 2021-12-03 江南大学 一种毕赤酵母中提高人源溶菌酶分泌效率及酶活的方法
EP4194560A1 (en) 2021-12-08 2023-06-14 European Molecular Biology Laboratory Improved production of secreted proteins in fungal cells

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
ATE438722T1 (de) 2000-05-17 2009-08-15 Mitsubishi Tanabe Pharma Corp Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein
DK1297172T3 (da) 2000-06-28 2006-02-13 Glycofi Inc Fremgangsmåder til frembringelse af modificerede glucoproteiner
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
US8697394B2 (en) 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7598055B2 (en) 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
GB0121088D0 (en) * 2001-08-31 2001-10-24 Cambridge Consultants Electric kettle
NZ533868A (en) 2001-12-27 2008-09-26 Glycofi Inc Methods to engineer mammalian-type carbohydrate structures
IL165717A0 (en) * 2002-06-26 2006-01-15 Flanders Interuniversity Inst A strain of methylotrophic yeast for producing proteins
EP1562417A4 (en) 2002-11-12 2007-08-29 Purdue Research Foundation BENZOATE-INDUCIBLE PROMOTERS
JP4861830B2 (ja) 2003-12-24 2012-01-25 グライコフィ， インコーポレイテッド 糖タンパク質の産生において、グリカンのマンノシルリン酸化を除去する方法
US7479389B2 (en) 2004-03-02 2009-01-20 Glycofi, Inc. ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration
EP2365089B1 (en) 2004-03-17 2014-05-14 GlycoFi, Inc. Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in yeast
CN101084233B (zh) 2004-04-29 2012-08-15 格利科菲公司 用于在糖蛋白产生中减少或消除α-甘露糖苷酶抗性聚糖的方法
US20060252069A1 (en) 2005-04-21 2006-11-09 Zhang Kunyan Pcr for mrsa sccmec typing
EP2027271A4 (en) 2006-05-19 2010-06-09 Glycofi Inc RECOMBINANT VECTORS
EP2032600A2 (en) * 2006-05-19 2009-03-11 Glycofi, Inc. Erythropoietin compositions

Also Published As

Publication number Publication date
AU2010313608A1 (en) 2012-06-07
WO2011053541A1 (en) 2011-05-05
KR20140015137A (ko) 2014-02-06
RU2012122166A (ru) 2013-12-10
JP2013509180A (ja) 2013-03-14
BR112012009886A2 (pt) 2015-09-15
CN102686731A (zh) 2012-09-19
CA2777487A1 (en) 2011-05-05
EP2494053A4 (en) 2013-10-30
EP2494053A1 (en) 2012-09-05
US20130011875A1 (en) 2013-01-10
MX2012004993A (es) 2012-06-12

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