US20050095724A1 - Chemical analyzer, method for dispensing and dilution cup - Google Patents

Chemical analyzer, method for dispensing and dilution cup Download PDF

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Publication number
US20050095724A1
US20050095724A1 US10/943,245 US94324504A US2005095724A1 US 20050095724 A1 US20050095724 A1 US 20050095724A1 US 94324504 A US94324504 A US 94324504A US 2005095724 A1 US2005095724 A1 US 2005095724A1
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United States
Prior art keywords
reagent
dispensing
sample solution
dispensed
container
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Abandoned
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US10/943,245
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English (en)
Inventor
Hitoshi Shibutani
Naoto Sato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toshiba Corp
Canon Medical Systems Corp
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Toshiba Corp
Toshiba Medical Systems Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toshiba Corp, Toshiba Medical Systems Corp filed Critical Toshiba Corp
Assigned to TOSHIBA MEDICAL SYSTEMS CORPORATION, KABUSHIKI KAISHA TOSHIBA reassignment TOSHIBA MEDICAL SYSTEMS CORPORATION ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: SATO, NAOTO, SHIBUTANI, HITOSHI
Publication of US20050095724A1 publication Critical patent/US20050095724A1/en
Priority to US12/106,906 priority Critical patent/US7897409B2/en
Priority to US12/716,332 priority patent/US8721966B2/en
Abandoned legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • G01N2035/00544—Mixing by a special element, e.g. stirrer using fluid flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1048—General features of the devices using the transfer device for another function
    • G01N2035/1058—General features of the devices using the transfer device for another function for mixing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/115831—Condition or time responsive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/115831—Condition or time responsive
    • Y10T436/116664—Condition or time responsive with automated titrator
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25625—Dilution
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Definitions

  • the present invention relates generally to a chemical analyzer, a method for dispensing and a dilution cup.
  • a chemical analyser automatically analyses a chemical composition included in blood or urine of a human body, for example.
  • a sample solution and a reagent are dispensed into a reaction cuvette.
  • the chemical analyser measures, as one of biochemistry test arrays, concentration/activity of a substance to be measured or enzyme in the sample solution by measuring amount of penetration of light in order to obtain a change of color tone caused by reaction of a mixed solution of the sample solution and the reagent corresponding to the biochemistry test array.
  • the chemical analyser measures concentration of sodium ion or potassium ion or chlorine ion in a sample solution dispensed into a dilution cup by measuring a change of electromotive force with an ion sensor about a mixed solution of the sample solution and a reagent.
  • the reagent is used for dilution of the sample solution or adjustment of pH.
  • the sample solution is dispensed into a container, and subsequently the reagent is dispensed into the container to be mixed with the sample solution.
  • the dispensing-first-sample solution method is classified into two methods, a non-contacting dispensing method and a contacting dispensing method.
  • the non-contacting dispensing method where the sample solution was dispensed into the container from a sample dispensing probe that was away from the container is applied.
  • the contact dispensing method the sample solution is dispensed into the container from a sample dispensing probe that is contacting the container has been applied, since the sample solution that should be dispensed into the container is adhered to the outside of the sample dispensing probe or flies in all directions and the sample solution is not dispensed into an appropriate part of the container in the non-contacting dispensing method.
  • the contacting dispensing method is described in Japanese Patent 61-56784 (KOUKOKU) (page 4-5 and FIG. 4-5).
  • the sample solution dispensed into the container does not spread into the container depending on the circumstances where stain remains or a bottom form where the sample dispensing probe contacts the container influences the spread. As described above, the sample solution is not dispensed well.
  • the reagent is first dispensed into the container, and the sample solution is dispensed into the container to be mixed with the reagent.
  • the dispensing-first reagent method is described in Japanese Patent Disclose (KOKAI) (page 3).
  • the dispensing-first-reagent method when the sample dispensing probe directly contacts the reagent, or the sample dispensing probe does not contact the reagent but a drop located on a top of the sample dispensing probe contacts a surface of the reagent, the sample solution is poured from the sample dispensing probe into the reagent.
  • the sample solution is not adhered to the outside of the sample dispensing probe or does not fly off, and therefore, the dispensing-first-reagent method is adapted at the present when the amount of the sample solution is little.
  • the dispensing-first-reagent method needs a more powerful mixing unit than that in the dispensing-first-sample solution method, in order to mix the sample solution and the reagent to be uniformed.
  • a speedy measurement or a speedy mixing is required recently, and it is difficult to achieve the required speed even with the powerful mixing unit.
  • the chemical analyzer it is required that the amount of the sample solution used for each biochemistry test array is reduced, and the sample solution is dispensed well.
  • the bottom form of the container or the remaining stain can be major error factor, and in the dispensing-first-reagent, the powerful mixing unit is required.
  • a method for dispensing a sample solution and a reagent into a container in a chemical analyser includes a first step of dispensing a reagent into a container, a second step of dispensing a sample solution into the container after the first step, and a third step of dispensing the reagent into the container after the second step.
  • a dilution cup includes an inner face where a diameter of a horizontal section increases on at least one position from a bottom to a top, an opening portion surrounding an opening, where a sample solution is dispensed, located on or near the top, a reagent aperture portion surrounding an aperture where a reagent is dispensed such that the sample solution and the reagent create an upswing spiral flow, and a drain aperture portion surrounding a drain aperture, where a mixed solution of the sample solution and the reagent is discharged, located on or near the bottom.
  • a chemical analyser includes a container where a sample solution and a reagent are mixed, a first mechanism configured to dispense the reagent into the container, a second mechanism configured to dispense the sample solution in to the container, and a controller configured to control the first mechanism to dispense the reagent in a first step, the second mechanism to dispense the sample solution in a second step after the first step, and the first mechanism to dispense the reagent in a third step after the second step.
  • FIG. 1 is a perspective view of a chemical analyzer according to an embodiment
  • FIG. 2 is a block diagram of an electrolyte measurement module according to the embodiment
  • FIGS. 3A through 3D are illustrations for explaining an operation of dispensing solution into a dilution cup according to the embodiment
  • FIGS. 4A through 4C are illustrations for explaining an operation of dispensing solution into a dilution cup according to a modification of the embodiment.
  • FIGS. 5A through 5C are illustrations for explaining an operation of dispensing solution into a reaction cuvette according to the embodiment.
  • FIGS. 1 through 5 an embodiment of a chemical analyzer, a method for dispensing and a dilution cup are explained.
  • FIG. 1 shows a perspective view of a chemical analyser of the embodiment.
  • the chemical analyser includes a reagent rack 1 for supporting a plurality of reagent bottles 7 where a reagent to be reacted with substance of a sample solution is included, reagent storages 2 and 3 which can hold the reagent rack 1 , a reaction dick 5 for supporting a plurality of reaction cuvettes.
  • the reaction cuvettes are supported on a circumference of the reaction disk 5 .
  • the chemical analyser includes a disk sampler 6 , a first reagent dispensing arm 8 , a second reagent dispensing arm 9 , a sample dispensing arm 10 , a photometric measurement unit 13 and an electrolyte measurement module 18 .
  • the disk sampler 6 supports a plurality of sample solution bottles 17 , each of which includes the sample solution are set.
  • the photometric measurement unit 13 measures a mixed solution of the reagent and the sample solution which are dispensed into reaction cuvette 4 via the reagent dispensing arm 8 or 9 and the sample dispensing arm 10 , respectively.
  • the electrolyte measurement module 18 measures a sample solution dispensed into a dilution cup 19 A via the sample dispensing arm 10 .
  • the reagent storages 2 and 3 , the reaction disk 5 and the disk sampler 6 are rotatable by a driving unit.
  • the reagent for the measurement is pulled out from the reagent bottle 7 and is dispensed into the reaction cuvette 4 by a first reagent probe 14 of the first reagent dispensing arm 8 or a second reagent probe 15 of the second reagent dispensing arm 9 .
  • the sample solution for the measurement is pulled out from the sample solution bottle 17 and is dispensed into the reaction cuvette 4 or the dilution cup 19 A by a sample dispensing probe 16 of the sample dispensing arm 10 .
  • the reaction cuvette 4 where the sample solution and the reagent are dispensed rotates and moves to such a position that a mixing unit 11 mixes the sample solution and the reagent.
  • the photometric measurement unit 13 irradiates a light to the reaction cuvette 4 which moves to a photometric measurement position and measures a change of a light absorption of the mixed solution.
  • at least one biochemistry test array other than sodium ion, potassium ion and chlorine ion in the sample solution is analysed.
  • the mixed solution of the sample solution and the reagent in the reaction cuvette 4 is evacuated, and the reaction cuvette 4 is washed by a washing machine 12 .
  • concentration of the electrolyte of the sodium ion or the potassium ion or the chlorine ion in a sample solution dispensed into the dilution cup 19 A is measured by the electrolyte measurement module 18 .
  • FIG. 2 shows the electrolyte measurement module 18 shown in FIG. 1 .
  • the electrolyte measurement module 18 includes a dilution cup unit 19 , a reagent supply unit 22 , a calibration solution supply unit 23 , a reagent suction unit 21 , and a ion sensor unit 20 .
  • the dilution cup unit 19 is used for mixing the sample solution and the reagent.
  • the reagent supply unit 22 and the calibration solution supply unit 23 supply the reagent and the calibration solution to the dilution cup unit 19 , respectively.
  • the reagent suction unit 21 supplies the mixed solution of the sample solution and the reagent or the calibration solution to the ion sensor unit 20 which measures the concentration of the electrolyte of the sodium ion or the potassium ion or the chlorine ion in the mixed solution of the sample solution and the reagent or the calibration solution.
  • the dilution cup unit 19 includes the dilution cup 19 A as the container for mixing the sample solution and the reagent, a reagent charge port 19 B used for dispensing the reagent into the dilution cup 19 A, and a calibration solution charge nozzle 19 C used for dispensing the calibration solution into the dilution cup 19 A.
  • the reagent supply unit 22 pulls out the reagent from a reagent bottle 22 B by using a reagent supply pump 22 A, and supplies the reagent into the dilution cup 19 to dilute the sample solution.
  • the calibration solution supply unit 23 pulls out the calibration solution from a calibration solution bottle 23 B by using a calibration solution supply pump 23 A, and supplies the calibration solution into the dilution cup 19 .
  • the calibration solution is used for washing the dilution cup 19 A to remove a remaining sample solution after the sample solution diluted in the dilution cup 19 A flows into the ion sensor unit 20 , and is used for calibrate an electromotive force of the ion sensor unit 20 .
  • a reagent suction pump 21 A leads the mixed solution diluted in the dilution cup 19 A or the calibration solution to the ion sensor unit 20 and subsequently the mixed solution or the calibration solution are evacuated into a waste solution tank 21 B.
  • the concentration of the electrolyte of the sodium ion, the potassium ion and the chlorine ion by a flow-through type composition electrode including a sodium ion sensor, a potassium ion sensor, a chlorine ion sensor and a reference electrode.
  • the following is a process for measuring the electrolyte.
  • the sample solution dispensed into the dilution cup 19 A by the sample dispensing probe 16 shown in FIG. 1 is mixed to the reagent supplied from the reagent supply unit 22 . Thereafter, the mixed solution is fed to the ion sensor unit 20 by the reagent suction unit 21 so that the electrolyte is measured.
  • the ion sensor unit 20 generates an electronic signal according to the concentration of a target ion.
  • the electronic signal is collected by a signal collection unit 24 , and is used for calculation process of the sample solution in a signal processor 25 .
  • the calibration solution is fed to the ion sensor unit 20 by the calibration solution supply unit 23 , and the electrolyte in the calibration solution is measured.
  • the concentration of the electrolyte is obtained.
  • each solution fed to the ion sensor unit 20 is evacuated by the reagent suction unit 21 .
  • An operation sequence of the reagent suction pump 21 A, the reagent supply pump 22 A, and the calibration solution supply pump 23 A, and a process of the signal generated in the ion sensor unit 20 are controlled by a controller according to a program stored in a memory unit of the chemical analyser in advance.
  • FIG. 3A through 3D show illustrations for explaining a process for dispensing the same reagent twice into the dilution cup 19 A.
  • FIG. 3A through 3C are sectional views of the dilution cup 19 A.
  • a drain aperture 32 to the ion sensor unit 20 is located at the bottom of the dilution cup.
  • a cross section which is substantially perpendicular to a vertical central axis 19 F which is located at a center of the dilution cup 19 A is circular form.
  • a diameter of the circular form gradually increases from the bottom to a top of the dilution cup 19 A.
  • a cross section which is substantially parallel to the vertical central axis in the inner face is a gradual curved surface, like a hanging bell.
  • the reagent charge port 19 B is located at a position slightly above the bottom of the dilution cup 19 A, and extends horizontally.
  • An reagent charge aperture 19 D connects the reagent charge port 19 B to the inner face of the dilution cup 19 A is located on a point on a circumference of the cross section which is substantially perpendicular to the vertical central axis 19 F.
  • FIG. 3A shows an illustration for explaining a first step for dispensing first predetermined amount of the reagent into the dilution cup 19 A.
  • the first predetermined amount 125 ⁇ l which is approximately 22% of the required amount is dispensed from the reagent charge port 19 B into the dilution cup 19 A.
  • the reagent 26 A is dispensed in the first step.
  • Speed for dispensing the reagent in the first step is controlled such that the reagent does not fly off from the dilution cup 19 A by the reagent supply pump 22 A.
  • FIG. 3B shows an illustration for explaining a second step for dispensing predetermined amount of the sample solution into the dilution cup 19 A after the first step.
  • the sample dispensing arm 10 shown in FIG. 1 rotates such that the sample dispensing probe 16 is located above the dilution cup 19 A and is located on the vertical central axis 19 F.
  • the sample dispensing probe 16 stops once at the position, and descends through an opening 31 which is located on the top of the dilution cup 19 A.
  • the sample dispensing probe 16 detects a surface of the reagent and stops at a depth of about 1 mm under the surface of the reagent to dispense 25 ⁇ l of the sample solution, for example.
  • an intermediate mixed solution of 25 ⁇ l of the sample solution and 125 ⁇ l of the reagent exists in the dilution cup 19 A.
  • the sample dispensing probe 16 soaks in the reagent 26 A dispensed in the first step when the sample solution is dispensed, as the dispensing-first-reagent method, the sample solution is not adhered to the outside of the sample dispensing probe 16 or does not fly off, and therefore, the dispensing is performed well.
  • FIG. 3C shows an illustration for explaining a third step for dispensing second predetermined amount of the reagent into the dilution cup 19 A.
  • the remaining amount 450 ⁇ l of the reagent is dispensed from the reagent charge port 19 B by the reagent supply pump 22 A to be uniformly mixed with the intermediate mixed solution 26 B. Finally, the mixed solution 26 C of the sample solution and the reagent exists.
  • the reagent 78% of the required reagent, namely 450 ⁇ l, which is more than that of the first step is dispensed.
  • the reagent is dispensed from the reagent charge aperture 19 D into the upside down hanging bell formed dilution cup 19 A under the control to create an upswing spiral flow indicated as an allow in FIG. 3C and FIG. 3D which shows an upper view of the dilution cup 19 A.
  • an effective mixing can be performed.
  • the mixed solution 26 C is fed to the ion sensor unit 20 via the drain aperture 32 by the reagent suction unit 21 so that the electrolyte is measured in the sample solution.
  • An operation of the sample dispensing arm 10 and the sample dispensing probe 16 is controlled by the controller according to a program stored in a memory unit of the chemical analyser in advance so as to work with the operation of each pump described above.
  • FIG. 4A through 4C show illustrations for explaining a process for dispensing the same reagent twice into a commonly used dilution cup 19 A, as a modification.
  • the difference between FIG. 3A through 3C and FIG. 4A through 4C is a position of a reagent charge port.
  • the reagent charge port 19 B is located near the bottom of the dilution cup 19 A, however in FIG. 4A through 4C , the reagent charge port 19 e is locate above the dilution cup 19 A.
  • FIG. 4A shows an illustration for explaining a first step for dispensing first predetermined amount of the reagent.
  • a required amount of the reagent is 575 ⁇ l
  • the first predetermined amount 125 ⁇ l which is approximately 22% of the required amount is dispensed from the reagent charge port 19 E into the dilution cup 19 A.
  • the reagent 27 A is dispensed in the first step.
  • Speed for dispensing the reagent in the first step is controlled such that the reagent does not fly off from the dilution cup 19 A by the reagent supply pump 22 A.
  • FIG. 4B shows an illustration for explaining a second step for dispensing predetermined amount of the sample solution into the dilution cup 19 A after the first step.
  • the sample dispensing arm 10 shown in FIG. 1 rotates such that the sample dispensing probe 16 is located above the dilution cup 19 A and is located on the vertical central axis 19 F.
  • the sample dispensing probe 16 stops once at the position, and descends through an opening 31 which is located on the top of the dilution cup 19 A.
  • the sample dispensing probe 16 detects a surface of the reagent and stops at a depth of about 1 mm under the surface of the reagent to dispense 25 ⁇ l of the sample solution, for example.
  • an intermediate mixed solution of 25 ⁇ l of the sample solution and 125 ⁇ l of the reagent exists in the dilution cup 19 A.
  • the sample dispensing probe 16 soaks in the reagent 27 A dispensed in the first step when the sample solution is dispensed, as the dispensing-first-reagent method, the sample solution is not adhered to the outside of the sample dispensing probe 16 or does not fly off, and therefore, the dispensing is performed well.
  • FIG. 4C shows an illustration for explaining a third step for dispensing second predetermined amount of the reagent into the dilution cup 19 A.
  • the remaining amount 450 ⁇ l of the reagent is dispensed from the reagent charge port 19 E by the reagent supply pump unit 22 to be uniformly mixed with the intermediate mixed solution 26 B. Finally, the mixed solution 27 C of the sample solution and the reagent exists.
  • the reagent 78% of the required reagent, namely 450 ⁇ l, which is more than that of the first step is dispensed.
  • the dispensing of the reagent is controlled such that the speed of the dispensing is as much as possible within a range where the reagent does not fly off.
  • the mixed solution 27 C is fed to the ion sensor unit 20 by the reagent suction unit 21 so that the electrolyte is measured in the sample solution.
  • the above mentioned method is effective especially to the electrolyte measurement where high precision of dispensing of the reagent and the sample solution is required.
  • FIG. 5A through 5C show illustrations for explaining a process for dispensing the same reagent twice into the reaction cuvette 4 .
  • FIG. 5A shows an illustration for explaining a first step for dispensing first predetermined amount of the reagent into the reaction cuvette 4 .
  • the first reagent probe 14 shown in FIG. 1 pulls out a first reagent corresponding to a biochemistry test array, and the first reagent dispensing arm 8 rotates such that the first reagent moves to a position for dispensing.
  • the lower limit of the amount of the first reagent which is required for photometric measurement of the photometric measurement unit 13 is 120 ⁇ l
  • one third of 120 ⁇ l, namely 40 ⁇ l of the first reagent is dispensed into an approximate center of the reaction cuvette 4 .
  • the reagent 28 A exists in the reaction cuvette 4 .
  • the reaction disk 5 rotates such that the reaction cuvette 4 moves to such a position that the sample solution is dispensed.
  • Speed of dispensing of the reagent in the first step is controlled not to fly off to a side inner face of the reaction cuvette 4 .
  • FIG. 5B shows an illustration for explaining a second step for dispensing predetermined amount of the sample solution into the reaction cuvette 4 after the first step.
  • the sample dispensing arm 10 shown in FIG. 1 rotates such that the sample dispensing probe 16 is located above a centor of the reaction cuvette 4 .
  • the sample dispensing probe 16 stops once at the position, descends to the reaction cuvette 4 , and detects a surface of the reagent 28 A.
  • the sample dispensing probe 16 stops at a depth of about 1 mm under the surface of the reagent 28 A to dispense the predetermined amount of the sample solution.
  • an intermediate mixed solution 28 B of the predetermined amount of the sample solution and the 40 ⁇ l of the reagent exists.
  • the reaction disk 5 rotates such that the reaction cuvette 4 is located at a position for dispensing the same reagent as that in the first step once again.
  • the above operation is controlled by a controller 30 .
  • the sample dispensing probe 16 soaks in the reagent 28 A dispensed in the first step when the sample solution is dispensed, as the dispensing-first-reagent method, the sample solution is not adhered to the outside of the sample dispensing probe 16 or does not fly off, and therefore, the dispensing is performed well.
  • FIG. 5C shows an illustration for explaining a third step for dispensing second predetermined amount of the reagent into the reaction cuvette 4 .
  • the first reagent probe 14 that pulls up the reagent fro the reagent storages 2 and rotates to be located at a second reagent dispensing position for dispensing the reagent once again.
  • the second predetermined amount of the reagent such as more than two thirds of the required amount, is dispensed into an approximate centor of the reaction cuvette 4 .
  • the second dispensed reagent is mixed with the intermediate mixed solution to be a mixed solution 28 C.
  • the reaction disk 5 rotates so that the reaction cuvette 4 moves to a position to be mixed by the mixing unit 11 .
  • the mixing unit 11 further mixes the mixed solution 28 C.
  • the second amount of the reagent which is more than that in the first step is dispensed, and speed of the dispensing is as much as possible within a range where the reagent does not fly off. Thereby, the reagent and the sample solution are mixed to be uniformed.
  • the reaction disk 5 rotates and stops, and the second reagent probe 15 pulls a second reagent corresponding to a biochemistry test array from the reagent storage 3 . Thereafter, the second reagent dispensing arm 9 rotates, and the second reagent is dispensed into the reaction cuvette 4 by the second reagent probe 15 .
  • the photometric measurement unit 13 irradiates the light to the reaction unit for a predetermined time, and detects the change of the light passing through the sample solution.
  • the above mentioned method is effective especially to the electrolyte measurement where high precision of dispensing of the reagent and the sample solution is required.
  • the chemical analyser may have a memory which stores information of relationship between the required total amount of the reagent and the amount of the reagent dispensed in the first step.
  • the speed of dispensing of the reagent in the first and third steps may be stored instead of or in addition to the amount relationship information.
  • the above mentioned separate dispensing method may be selected by each biochemistry test array.
  • the speed of dispensing of the reagent in the third step may be faster than that of the first step, and thereby, more effective mixing can be performed.

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  • Life Sciences & Earth Sciences (AREA)
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  • Analytical Chemistry (AREA)
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US12/106,906 US7897409B2 (en) 2003-09-19 2008-04-21 Chemical analyzer, method for dispensing and dilution cup
US12/716,332 US8721966B2 (en) 2003-09-19 2010-03-03 Chemical analyzer, method for dispensing and dilution cup

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US20080085215A1 (en) * 2006-10-10 2008-04-10 Sysmex Corporation Analyzer
US7469606B1 (en) * 2004-08-26 2008-12-30 Elemental Scientific, Inc. Automated sampling device
US20090114538A1 (en) * 2007-11-07 2009-05-07 Takayama Hiroko Automated biochemical analyzer
US7637175B1 (en) 2004-08-26 2009-12-29 Elemental Scientific, Inc. Automated sampling device
US7690275B1 (en) 2004-08-26 2010-04-06 Elemental Scientific, Inc. Automated sampling device
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US7897409B2 (en) 2011-03-01
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US20090202389A1 (en) 2009-08-13
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