RU94017674A - Способ получения соединения, вектор, клетка - Google Patents
Способ получения соединения, вектор, клеткаInfo
- Publication number
- RU94017674A RU94017674A RU94017674/13A RU94017674A RU94017674A RU 94017674 A RU94017674 A RU 94017674A RU 94017674/13 A RU94017674/13 A RU 94017674/13A RU 94017674 A RU94017674 A RU 94017674A RU 94017674 A RU94017674 A RU 94017674A
- Authority
- RU
- Russia
- Prior art keywords
- adipoyl
- adca
- apa
- vector
- cell
- Prior art date
Links
- 150000001875 compounds Chemical class 0.000 title 1
- NVIAYEIXYQCDAN-CLZZGJSISA-N 7β-aminodeacetoxycephalosporanic acid Chemical compound S1CC(C)=C(C(O)=O)N2C(=O)[C@@H](N)[C@@H]12 NVIAYEIXYQCDAN-CLZZGJSISA-N 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 2
- 125000003651 hexanedioyl group Chemical group C(CCCCC(=O)*)(=O)* 0.000 claims 2
- 229940064005 Antibiotic throat preparations Drugs 0.000 claims 1
- 229940083879 Antibiotics FOR TREATMENT OF HEMORRHOIDS AND ANAL FISSURES FOR TOPICAL USE Drugs 0.000 claims 1
- 229940042052 Antibiotics for systemic use Drugs 0.000 claims 1
- 229940042786 Antitubercular Antibiotics Drugs 0.000 claims 1
- 101710029063 CEFEF Proteins 0.000 claims 1
- 229940093922 Gynecological Antibiotics Drugs 0.000 claims 1
- 241000228150 Penicillium chrysogenum Species 0.000 claims 1
- 241000187433 Streptomyces clavuligerus Species 0.000 claims 1
- 229940024982 Topical Antifungal Antibiotics Drugs 0.000 claims 1
- WNLRTRBMVRJNCN-UHFFFAOYSA-L adipate(2-) Chemical compound [O-]C(=O)CCCCC([O-])=O WNLRTRBMVRJNCN-UHFFFAOYSA-L 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 230000003115 biocidal Effects 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 101700034534 cefE Proteins 0.000 claims 1
- 150000001780 cephalosporins Chemical class 0.000 claims 1
- 239000012467 final product Substances 0.000 claims 1
- 238000011065 in-situ storage Methods 0.000 claims 1
- 229940079866 intestinal antibiotics Drugs 0.000 claims 1
- 229940005935 ophthalmologic Antibiotics Drugs 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 230000002194 synthesizing Effects 0.000 claims 1
Claims (1)
- Предлагается промежуточное соединение для получения цефалоспориновых антибиотиков, 7-аминодезацетоксицэфалоспориновую кислоту (7-ADCA), получают с помощью нового биопроцесса, в котором выращивают штамм трансформированного Penicillium chrysogenum в присутствии адипинатного питания, и получают адипоил-6-АРА (6-аминопенициллиновую кислоту), и insitu вектор экспрессии гена экспандазы, например, из streptomyces clavuligerus, с которым трансформирован Р. chrysogпenum, превращает адипоил-6-АРА, путем расширения кольца, в адипоил-7-ADCA. Конечный продукт - 7-ADCA - получают затем путем отщепления адипоильной боковой цепи, используя адипоилацилазу. Соответственно, используя биопроцесс, осуществляют полный синтез, являющийся эффективным и экономичным.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US75787991A | 1991-09-11 | 1991-09-11 | |
US757,879 | 1991-09-11 | ||
US933,469 | 1992-08-28 | ||
US07/933,469 US5318896A (en) | 1991-09-11 | 1992-08-28 | Recombinant expandase bioprocess for preparing 7-aminodesacetoxy cephalosporanic acid (7-ADCA) |
Publications (2)
Publication Number | Publication Date |
---|---|
RU94017674A true RU94017674A (ru) | 1996-03-10 |
RU2178808C2 RU2178808C2 (ru) | 2002-01-27 |
Family
ID=27116462
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU94017674/13A RU2178808C2 (ru) | 1991-09-11 | 1992-09-11 | Способ получения 7-аминодезацетоксицефалоспорановой кислоты (7-adca) |
Country Status (25)
Country | Link |
---|---|
US (1) | US5318896A (ru) |
EP (2) | EP0843013A3 (ru) |
JP (2) | JP3027847B2 (ru) |
KR (2) | KR0132440B1 (ru) |
CN (2) | CN1066488C (ru) |
AT (1) | ATE173017T1 (ru) |
AU (1) | AU657787B2 (ru) |
BG (1) | BG62177B1 (ru) |
CA (1) | CA2077921C (ru) |
CZ (1) | CZ287357B6 (ru) |
DE (1) | DE69227494T2 (ru) |
DK (1) | DK0532341T3 (ru) |
ES (1) | ES2126588T3 (ru) |
FI (1) | FI108148B (ru) |
HU (1) | HU217171B (ru) |
IL (1) | IL103076A (ru) |
MX (1) | MX9205175A (ru) |
NO (1) | NO316742B1 (ru) |
NZ (1) | NZ244236A (ru) |
RO (1) | RO115886B1 (ru) |
RU (1) | RU2178808C2 (ru) |
SK (1) | SK282387B6 (ru) |
TW (1) | TW270149B (ru) |
UA (1) | UA41292C2 (ru) |
WO (1) | WO1993005158A1 (ru) |
Families Citing this family (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0233715B1 (en) * | 1986-01-31 | 1994-05-25 | BEECHAM GROUP plc | Isolation and expression of genes involved in the biosynthesis of beta-lactams |
US6709859B1 (en) | 1986-01-31 | 2004-03-23 | Beecham Group P.L.C. | Chromosomal DNA Fragments Encoding Enzymes for Encoding B-Lactam Biosynthesis Enzymes, and Vector and Transformants for Their Expression |
CZ288721B6 (cs) * | 1991-10-15 | 2001-08-15 | Dsm Gist B. V. | Biologický způsob výroby kyseliny 7-aminodeacetylcefalosporanové |
US5726032A (en) * | 1993-07-30 | 1998-03-10 | Gist-Brocades B.V. | Process for the efficient production of 7-ADCA via 2-(carboxyethylthio)acetyl-7-ADCA and 3-(carboxymethylthio)propionyl-7-ADCA |
JP3574453B2 (ja) * | 1993-07-30 | 2004-10-06 | ギスト ブロカデス ベスローテン フェンノートシャップ | 3‐(カルボキシエチルチオ)プロピオニル‐7‐adcaを経由した7‐adcaの効果的な製造法 |
US5731165A (en) * | 1995-06-02 | 1998-03-24 | Gist-Brocades, B.V. | Process for the production of 7-ADCA via expandase activity on penicillin G |
WO1997020053A2 (en) * | 1995-11-27 | 1997-06-05 | Gist-Brocades B.V. | Improved process for the production of semi-synthetic cephalosporins via expandase activity on penicillin g |
AU2508897A (en) * | 1996-04-04 | 1997-10-29 | Gist-Brocades B.V. | An enzyme with high adipoyl-coenzyme a synthetase activity and uses thereof |
EP0924180A4 (en) | 1996-07-02 | 2005-04-20 | Toray Finechemicals Co Ltd | PROCESS FOR THE PRODUCTION OF OPTICALLY ACTIVE KETONES |
AU4201897A (en) | 1996-07-16 | 1998-02-09 | Gist-Brocades B.V. | Novel process for the preparation of cephalosporins using (acremonium chrysogenum) |
AU6400098A (en) * | 1997-02-20 | 1998-09-09 | Gist-Brocades B.V. | Fermentative production of valuable compounds on an industrial scale using chemically defined media |
ZA983396B (en) * | 1997-04-22 | 1998-10-27 | Gist Brocades Bv | A method for controlling the solubility of a beta-lactam nucleus |
KR20000022106A (ko) * | 1997-04-22 | 2000-04-25 | 데 보어 빌렘 뢸프 | β-락탐 항생물질의 제조방법 |
AU7648898A (en) * | 1997-04-22 | 1998-11-13 | Gist-Brocades B.V. | Process for the fermentative production of deacylated cephalosporins |
US6410259B1 (en) * | 1997-04-22 | 2002-06-25 | Dsm Patents & Trademarks | Process for the fermentative production of deacylated cephalosporins |
EP0918878B1 (en) | 1997-04-22 | 2005-02-09 | DSM IP Assets B.V. | Improved process for the fermentative production of cephalosporin |
CA2316401A1 (en) * | 1997-12-24 | 1999-07-08 | Isis Innovation Limited | Modified deacetoxycephalosporin c synthase (daocs) and x-ray structure |
RU2219182C2 (ru) * | 1998-03-27 | 2003-12-20 | Дсм Н.В. | Новый способ ферментативного получения цефалоспорина |
US6383773B2 (en) * | 1998-04-23 | 2002-05-07 | Massachusetts Institute Of Technology | Penicillin conversion |
KR100648480B1 (ko) | 1998-05-19 | 2006-11-24 | 디에스엠 아이피 어셋츠 비.브이. | 세팔로스포린의 개선된 생체내 생산 |
ES2165276B1 (es) * | 1999-06-18 | 2003-03-01 | Antibioticos Sau | Procedimiento biotecnologico para la produccion de acido 7-aminocefal osporanico (7-adca) y compuestos de sintesis intermedios particularmente penicilina n y desacetoxicefalosporina c (daoc) |
ATE273982T1 (de) | 2000-05-13 | 2004-09-15 | Smithkline Beecham Plc | Verfahren zur reinigung eines salzes der clavulansäure |
WO2007073947A2 (en) | 2005-12-28 | 2007-07-05 | Dsm Ip Assets B.V. | Mutant type ii beta- lactam acylases |
EP1987041B1 (en) * | 2006-02-23 | 2013-05-22 | DSM Sinochem Pharmaceuticals Netherlands B.V. | Improved cephalosporin production |
EP2123772A1 (en) | 2008-04-29 | 2009-11-25 | DSM IP Assets B.V. | Beta-lactam antibiotic producing strains |
EP2392649A3 (en) | 2008-08-05 | 2012-01-11 | DSM IP Assets B.V. | Adipoyl-7-ADCA producing strains |
WO2010015625A1 (en) * | 2008-08-05 | 2010-02-11 | Dsm Ip Assets B.V. | Adipoyl-7-adca producing strains |
WO2010015624A1 (en) * | 2008-08-05 | 2010-02-11 | Dsm Ip Assets B.V. | Adipoyl-7-adca producing strains |
CN102369290A (zh) | 2009-04-03 | 2012-03-07 | 帝斯曼知识产权资产管理有限公司 | 发酵工艺 |
CN103003439A (zh) | 2010-06-22 | 2013-03-27 | 中化帝斯曼制药有限公司荷兰公司 | 气泡发酵工艺 |
CN103108879B (zh) | 2010-09-07 | 2016-08-17 | 中化帝斯曼制药有限公司荷兰公司 | 用于生产头孢菌素类的方法 |
BR112013022521B1 (pt) | 2011-03-03 | 2019-05-07 | Dsm Sinochem Pharmaceuticals Netherlands B.V. | PROCESSO PARA A DEGRADAÇÃO DE UM COMPOSTO ß-LACTÂMICO E USO DE SULFITO NA DEGRADAÇÃO DE UM COMPOSTO ß-LACTÂMICO |
WO2015091455A1 (en) | 2013-12-17 | 2015-06-25 | Dpx Holdings B.V. | Bioreactor |
CN113699209B (zh) * | 2021-08-30 | 2023-05-02 | 浙江昂利康制药股份有限公司 | 一种7-adca回收方法 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR1270852A (fr) * | 1960-09-20 | 1961-09-01 | Bayer Ag | Procédé d'acylation enzymatique d'acide 6-aminopénicillanique |
EP0233715B1 (en) * | 1986-01-31 | 1994-05-25 | BEECHAM GROUP plc | Isolation and expression of genes involved in the biosynthesis of beta-lactams |
HUT43644A (en) * | 1986-11-17 | 1987-11-30 | Univ Oxford | Process for producing 3-substituted-cephalosporines |
ES2058141T3 (es) * | 1987-01-17 | 1994-11-01 | Hoechst Ag | Utilizacion de gamma-glutamil-transpeptidasa. |
US5070020A (en) * | 1988-05-09 | 1991-12-03 | Eli Lilly And Company | Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase |
US5108918A (en) * | 1988-08-11 | 1992-04-28 | Gist-Brocades | Method for identifying and using biosynthetic genes for enhanced production of secondary metabolites |
IL95766A (en) * | 1989-09-27 | 1996-12-05 | Lilly Co Eli | Recombinant DNA expression vectors And DNA compounds. Which encode Aspergillus acyltransferase activity |
KR0171897B1 (ko) * | 1989-12-27 | 1999-02-01 | 후지사와 도모끼찌로 | 7-아미노세펨 화합물 또는 그 염류의 제조 방법 |
CZ288721B6 (cs) * | 1991-10-15 | 2001-08-15 | Dsm Gist B. V. | Biologický způsob výroby kyseliny 7-aminodeacetylcefalosporanové |
-
1992
- 1992-08-28 US US07/933,469 patent/US5318896A/en not_active Expired - Lifetime
- 1992-09-07 NZ NZ244236A patent/NZ244236A/en not_active IP Right Cessation
- 1992-09-07 IL IL10307692A patent/IL103076A/en not_active IP Right Cessation
- 1992-09-10 CN CN92112278A patent/CN1066488C/zh not_active Expired - Lifetime
- 1992-09-10 CA CA002077921A patent/CA2077921C/en not_active Expired - Fee Related
- 1992-09-10 MX MX9205175A patent/MX9205175A/es unknown
- 1992-09-11 EP EP97203374A patent/EP0843013A3/en not_active Withdrawn
- 1992-09-11 DK DK92308286T patent/DK0532341T3/da active
- 1992-09-11 AU AU23542/92A patent/AU657787B2/en not_active Ceased
- 1992-09-11 ES ES92308286T patent/ES2126588T3/es not_active Expired - Lifetime
- 1992-09-11 AT AT92308286T patent/ATE173017T1/de not_active IP Right Cessation
- 1992-09-11 UA UA94005434A patent/UA41292C2/ru unknown
- 1992-09-11 RO RO94-00380A patent/RO115886B1/ro unknown
- 1992-09-11 DE DE69227494T patent/DE69227494T2/de not_active Expired - Fee Related
- 1992-09-11 JP JP5505516A patent/JP3027847B2/ja not_active Expired - Fee Related
- 1992-09-11 RU RU94017674/13A patent/RU2178808C2/ru not_active IP Right Cessation
- 1992-09-11 EP EP92308286A patent/EP0532341B1/en not_active Expired - Lifetime
- 1992-09-11 KR KR1019940700777A patent/KR0132440B1/ko active
- 1992-09-11 KR KR1019940700777A patent/KR940702544A/ko not_active IP Right Cessation
- 1992-09-11 SK SK288-94A patent/SK282387B6/sk unknown
- 1992-09-11 HU HU9400715A patent/HU217171B/hu not_active IP Right Cessation
- 1992-09-11 CZ CZ1994532A patent/CZ287357B6/cs not_active IP Right Cessation
- 1992-09-11 WO PCT/US1992/007711 patent/WO1993005158A1/en active IP Right Grant
- 1992-09-19 TW TW081107399A patent/TW270149B/zh active
-
1994
- 1994-03-09 BG BG98643A patent/BG62177B1/bg unknown
- 1994-03-10 NO NO19940848A patent/NO316742B1/no unknown
- 1994-03-10 FI FI941135A patent/FI108148B/fi active
-
1999
- 1999-08-26 JP JP11239731A patent/JP3072101B2/ja not_active Expired - Fee Related
-
2000
- 2000-08-31 CN CN00126889A patent/CN1332247A/zh active Pending
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