RU94017674A - Способ получения соединения, вектор, клетка - Google Patents

Способ получения соединения, вектор, клетка

Info

Publication number
RU94017674A
RU94017674A RU94017674/13A RU94017674A RU94017674A RU 94017674 A RU94017674 A RU 94017674A RU 94017674/13 A RU94017674/13 A RU 94017674/13A RU 94017674 A RU94017674 A RU 94017674A RU 94017674 A RU94017674 A RU 94017674A
Authority
RU
Russia
Prior art keywords
adipoyl
adca
apa
vector
cell
Prior art date
Application number
RU94017674/13A
Other languages
English (en)
Other versions
RU2178808C2 (ru
Inventor
Дж. КОНДЕР Майкл
КРОФОРД Лорили
К. МакЭЙДА Филлис
А. РЭМБОУСЕК Джон
Original Assignee
Мерк Энд Ко., Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/933,469 external-priority patent/US5318896A/en
Application filed by Мерк Энд Ко., Инк. filed Critical Мерк Энд Ко., Инк.
Publication of RU94017674A publication Critical patent/RU94017674A/ru
Application granted granted Critical
Publication of RU2178808C2 publication Critical patent/RU2178808C2/ru

Links

Claims (1)

  1. Предлагается промежуточное соединение для получения цефалоспориновых антибиотиков, 7-аминодезацетоксицэфалоспориновую кислоту (7-ADCA), получают с помощью нового биопроцесса, в котором выращивают штамм трансформированного Penicillium chrysogenum в присутствии адипинатного питания, и получают адипоил-6-АРА (6-аминопенициллиновую кислоту), и insitu вектор экспрессии гена экспандазы, например, из streptomyces clavuligerus, с которым трансформирован Р. chrysogпenum, превращает адипоил-6-АРА, путем расширения кольца, в адипоил-7-ADCA. Конечный продукт - 7-ADCA - получают затем путем отщепления адипоильной боковой цепи, используя адипоилацилазу. Соответственно, используя биопроцесс, осуществляют полный синтез, являющийся эффективным и экономичным.
RU94017674/13A 1991-09-11 1992-09-11 Способ получения 7-аминодезацетоксицефалоспорановой кислоты (7-adca) RU2178808C2 (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US75787991A 1991-09-11 1991-09-11
US757,879 1991-09-11
US933,469 1992-08-28
US07/933,469 US5318896A (en) 1991-09-11 1992-08-28 Recombinant expandase bioprocess for preparing 7-aminodesacetoxy cephalosporanic acid (7-ADCA)

Publications (2)

Publication Number Publication Date
RU94017674A true RU94017674A (ru) 1996-03-10
RU2178808C2 RU2178808C2 (ru) 2002-01-27

Family

ID=27116462

Family Applications (1)

Application Number Title Priority Date Filing Date
RU94017674/13A RU2178808C2 (ru) 1991-09-11 1992-09-11 Способ получения 7-аминодезацетоксицефалоспорановой кислоты (7-adca)

Country Status (25)

Country Link
US (1) US5318896A (ru)
EP (2) EP0843013A3 (ru)
JP (2) JP3027847B2 (ru)
KR (2) KR0132440B1 (ru)
CN (2) CN1066488C (ru)
AT (1) ATE173017T1 (ru)
AU (1) AU657787B2 (ru)
BG (1) BG62177B1 (ru)
CA (1) CA2077921C (ru)
CZ (1) CZ287357B6 (ru)
DE (1) DE69227494T2 (ru)
DK (1) DK0532341T3 (ru)
ES (1) ES2126588T3 (ru)
FI (1) FI108148B (ru)
HU (1) HU217171B (ru)
IL (1) IL103076A (ru)
MX (1) MX9205175A (ru)
NO (1) NO316742B1 (ru)
NZ (1) NZ244236A (ru)
RO (1) RO115886B1 (ru)
RU (1) RU2178808C2 (ru)
SK (1) SK282387B6 (ru)
TW (1) TW270149B (ru)
UA (1) UA41292C2 (ru)
WO (1) WO1993005158A1 (ru)

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EP0233715B1 (en) * 1986-01-31 1994-05-25 BEECHAM GROUP plc Isolation and expression of genes involved in the biosynthesis of beta-lactams
US6709859B1 (en) 1986-01-31 2004-03-23 Beecham Group P.L.C. Chromosomal DNA Fragments Encoding Enzymes for Encoding B-Lactam Biosynthesis Enzymes, and Vector and Transformants for Their Expression
CZ288721B6 (cs) * 1991-10-15 2001-08-15 Dsm Gist B. V. Biologický způsob výroby kyseliny 7-aminodeacetylcefalosporanové
US5726032A (en) * 1993-07-30 1998-03-10 Gist-Brocades B.V. Process for the efficient production of 7-ADCA via 2-(carboxyethylthio)acetyl-7-ADCA and 3-(carboxymethylthio)propionyl-7-ADCA
JP3574453B2 (ja) * 1993-07-30 2004-10-06 ギスト ブロカデス ベスローテン フェンノートシャップ ３‐（カルボキシエチルチオ）プロピオニル‐７‐ａｄｃａを経由した７‐ａｄｃａの効果的な製造法
US5731165A (en) * 1995-06-02 1998-03-24 Gist-Brocades, B.V. Process for the production of 7-ADCA via expandase activity on penicillin G
WO1997020053A2 (en) * 1995-11-27 1997-06-05 Gist-Brocades B.V. Improved process for the production of semi-synthetic cephalosporins via expandase activity on penicillin g
AU2508897A (en) * 1996-04-04 1997-10-29 Gist-Brocades B.V. An enzyme with high adipoyl-coenzyme a synthetase activity and uses thereof
EP0924180A4 (en) 1996-07-02 2005-04-20 Toray Finechemicals Co Ltd PROCESS FOR THE PRODUCTION OF OPTICALLY ACTIVE KETONES
AU4201897A (en) 1996-07-16 1998-02-09 Gist-Brocades B.V. Novel process for the preparation of cephalosporins using (acremonium chrysogenum)
AU6400098A (en) * 1997-02-20 1998-09-09 Gist-Brocades B.V. Fermentative production of valuable compounds on an industrial scale using chemically defined media
ZA983396B (en) * 1997-04-22 1998-10-27 Gist Brocades Bv A method for controlling the solubility of a beta-lactam nucleus
KR20000022106A (ko) * 1997-04-22 2000-04-25 데 보어 빌렘 뢸프 β-락탐 항생물질의 제조방법
AU7648898A (en) * 1997-04-22 1998-11-13 Gist-Brocades B.V. Process for the fermentative production of deacylated cephalosporins
US6410259B1 (en) * 1997-04-22 2002-06-25 Dsm Patents & Trademarks Process for the fermentative production of deacylated cephalosporins
EP0918878B1 (en) 1997-04-22 2005-02-09 DSM IP Assets B.V. Improved process for the fermentative production of cephalosporin
CA2316401A1 (en) * 1997-12-24 1999-07-08 Isis Innovation Limited Modified deacetoxycephalosporin c synthase (daocs) and x-ray structure
RU2219182C2 (ru) * 1998-03-27 2003-12-20 Дсм Н.В. Новый способ ферментативного получения цефалоспорина
US6383773B2 (en) * 1998-04-23 2002-05-07 Massachusetts Institute Of Technology Penicillin conversion
KR100648480B1 (ko) 1998-05-19 2006-11-24 디에스엠 아이피 어셋츠 비.브이. 세팔로스포린의 개선된 생체내 생산
ES2165276B1 (es) * 1999-06-18 2003-03-01 Antibioticos Sau Procedimiento biotecnologico para la produccion de acido 7-aminocefal osporanico (7-adca) y compuestos de sintesis intermedios particularmente penicilina n y desacetoxicefalosporina c (daoc)
ATE273982T1 (de) 2000-05-13 2004-09-15 Smithkline Beecham Plc Verfahren zur reinigung eines salzes der clavulansäure
WO2007073947A2 (en) 2005-12-28 2007-07-05 Dsm Ip Assets B.V. Mutant type ii beta- lactam acylases
EP1987041B1 (en) * 2006-02-23 2013-05-22 DSM Sinochem Pharmaceuticals Netherlands B.V. Improved cephalosporin production
EP2123772A1 (en) 2008-04-29 2009-11-25 DSM IP Assets B.V. Beta-lactam antibiotic producing strains
EP2392649A3 (en) 2008-08-05 2012-01-11 DSM IP Assets B.V. Adipoyl-7-ADCA producing strains
WO2010015625A1 (en) * 2008-08-05 2010-02-11 Dsm Ip Assets B.V. Adipoyl-7-adca producing strains
WO2010015624A1 (en) * 2008-08-05 2010-02-11 Dsm Ip Assets B.V. Adipoyl-7-adca producing strains
CN102369290A (zh) 2009-04-03 2012-03-07 帝斯曼知识产权资产管理有限公司 发酵工艺
CN103003439A (zh) 2010-06-22 2013-03-27 中化帝斯曼制药有限公司荷兰公司 气泡发酵工艺
CN103108879B (zh) 2010-09-07 2016-08-17 中化帝斯曼制药有限公司荷兰公司 用于生产头孢菌素类的方法
BR112013022521B1 (pt) 2011-03-03 2019-05-07 Dsm Sinochem Pharmaceuticals Netherlands B.V. PROCESSO PARA A DEGRADAÇÃO DE UM COMPOSTO ß-LACTÂMICO E USO DE SULFITO NA DEGRADAÇÃO DE UM COMPOSTO ß-LACTÂMICO
WO2015091455A1 (en) 2013-12-17 2015-06-25 Dpx Holdings B.V. Bioreactor
CN113699209B (zh) * 2021-08-30 2023-05-02 浙江昂利康制药股份有限公司 一种7-adca回收方法

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FR1270852A (fr) * 1960-09-20 1961-09-01 Bayer Ag Procédé d'acylation enzymatique d'acide 6-aminopénicillanique
EP0233715B1 (en) * 1986-01-31 1994-05-25 BEECHAM GROUP plc Isolation and expression of genes involved in the biosynthesis of beta-lactams
HUT43644A (en) * 1986-11-17 1987-11-30 Univ Oxford Process for producing 3-substituted-cephalosporines
ES2058141T3 (es) * 1987-01-17 1994-11-01 Hoechst Ag Utilizacion de gamma-glutamil-transpeptidasa.
US5070020A (en) * 1988-05-09 1991-12-03 Eli Lilly And Company Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase
US5108918A (en) * 1988-08-11 1992-04-28 Gist-Brocades Method for identifying and using biosynthetic genes for enhanced production of secondary metabolites
IL95766A (en) * 1989-09-27 1996-12-05 Lilly Co Eli Recombinant DNA expression vectors And DNA compounds. Which encode Aspergillus acyltransferase activity
KR0171897B1 (ko) * 1989-12-27 1999-02-01 후지사와 도모끼찌로 7-아미노세펨 화합물 또는 그 염류의 제조 방법
CZ288721B6 (cs) * 1991-10-15 2001-08-15 Dsm Gist B. V. Biologický způsob výroby kyseliny 7-aminodeacetylcefalosporanové

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