RU2011103197A - Дифференцирование плюрипотентных стволовых клеток - Google Patents

Дифференцирование плюрипотентных стволовых клеток Download PDF

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RU2011103197A
RU2011103197A RU2011103197/10A RU2011103197A RU2011103197A RU 2011103197 A RU2011103197 A RU 2011103197A RU 2011103197/10 A RU2011103197/10 A RU 2011103197/10A RU 2011103197 A RU2011103197 A RU 2011103197A RU 2011103197 A RU2011103197 A RU 2011103197A
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pdgf
pluripotent stem
stem cells
pyridinotriazine
compound selected
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RU2533805C2 (ru
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Цзяцзянь ЛЮ (US)
Цзяцзянь ЛЮ
Джанет ДЭВИС (US)
Джанет ДЭВИС
Кристин ПАРМЕНТЕР (US)
Кристин ПАРМЕНТЕР
Паскаль Гислен Андрэ БОННЕТ (BE)
Паскаль Гислен Андрэ БОННЕТ
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Сентокор Орто Байотек Инк. (Us)
Сентокор Орто Байотек Инк.
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Abstract

1. Способ дифференцирования плюрипотентных стволовых клеток в клетки, экспрессирующие маркеры, характерные для линии сформированной эндодермы, включающий обработку плюрипотентных стволовых клеток средой, не содержащей активин А, но содержащей соединение, выбираемое из: EGF, FGF4, PDGF-A, PDGF-B, PDGF-C, PDGF-D, VEGF, GDF-8, мусцимола, PD98059, LY294002, U0124, U0126 и бутирата натрия, в течение периода времени, достаточного для того, чтобы плюрипотентные стволовые клетки дифференцировались в клетки, экспрессирующие маркеры, характерные для линии сформированной эндодермы. ! 2. Способ по п.1, в котором среда, не содержащая активин А, также содержит по меньшей мере одно другое соединение, выбираемое из группы, включающей анилин-пиридинотриазин и циклический анилин-пиридинотриазин.

Claims (2)

1. Способ дифференцирования плюрипотентных стволовых клеток в клетки, экспрессирующие маркеры, характерные для линии сформированной эндодермы, включающий обработку плюрипотентных стволовых клеток средой, не содержащей активин А, но содержащей соединение, выбираемое из: EGF, FGF4, PDGF-A, PDGF-B, PDGF-C, PDGF-D, VEGF, GDF-8, мусцимола, PD98059, LY294002, U0124, U0126 и бутирата натрия, в течение периода времени, достаточного для того, чтобы плюрипотентные стволовые клетки дифференцировались в клетки, экспрессирующие маркеры, характерные для линии сформированной эндодермы.
2. Способ по п.1, в котором среда, не содержащая активин А, также содержит по меньшей мере одно другое соединение, выбираемое из группы, включающей анилин-пиридинотриазин и циклический анилин-пиридинотриазин.
RU2011103197/10A 2008-06-30 2009-06-30 Дифференцирование плюрипотентных стволовых клеток, представляющих собой линию клеток человека RU2533805C2 (ru)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US7690808P 2008-06-30 2008-06-30
US7691508P 2008-06-30 2008-06-30
US7690008P 2008-06-30 2008-06-30
US61/076,915 2008-06-30
US61/076,900 2008-06-30
US61/076,908 2008-06-30
PCT/US2009/049183 WO2010002846A1 (en) 2008-06-30 2009-06-30 Differentiation of pluripotent stem cells

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KR (4) KR101829310B1 (ru)
CN (1) CN102159703B (ru)
AU (1) AU2009267137A1 (ru)
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CA (1) CA2729121C (ru)
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