MXPA02009339A - Plasmido recombinante capaz de liberarse en forma circular cerrada a partir de una secuencia nucleiotidica mas grande que permite la expresion especifica de sitio o la expresion regulada en el desarrollo de secuenicas geneticas seleccionadas. - Google Patents

Plasmido recombinante capaz de liberarse en forma circular cerrada a partir de una secuencia nucleiotidica mas grande que permite la expresion especifica de sitio o la expresion regulada en el desarrollo de secuenicas geneticas seleccionadas.

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Publication number
MXPA02009339A
MXPA02009339A MXPA02009339A MXPA02009339A MXPA02009339A MX PA02009339 A MXPA02009339 A MX PA02009339A MX PA02009339 A MXPA02009339 A MX PA02009339A MX PA02009339 A MXPA02009339 A MX PA02009339A MX PA02009339 A MXPA02009339 A MX PA02009339A
Authority
MX
Mexico
Prior art keywords
expression
sequence
release
protein
site specific
Prior art date
Application number
MXPA02009339A
Other languages
English (en)
Inventor
Robert Maxwell Harding
Original Assignee
Univ Queensland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AUPQ6516A external-priority patent/AUPQ651600A0/en
Priority claimed from AUPR1081A external-priority patent/AUPR108100A0/en
Application filed by Univ Queensland filed Critical Univ Queensland
Publication of MXPA02009339A publication Critical patent/MXPA02009339A/es

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8263—Ablation; Apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Arrangement And Driving Of Transmission Devices (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invencion se relaciona generalmente con plasmidos recombinantes y en particular plasmidos recombinantes geneticos que comprenden secuencias polinucleotidicas capaces de liberarse en forma circular, cerrada covalentemente a partir de una secuencia nucleotidica mas grande tal como, pero sin limitarse a un genoma de una celula eucariota. Preferiblemente, una vez liberada, la secuencia polinucleotidica se reconstituye en una forma que permite la expresion de la secuencia polinucleotidica. En una modalidad, la secuencia polinucleotidica reconstituida comprende una secuencia codificante con la totalidad o parte de un nucleotido extrano tal como, pero sin limitarse a una secuencia intronica u otra senal de maduracion insertada en la misma. La expresion y en particular la transcripcion de la secuencia codificante involucra la maduracion de la secuencia extrana. La liberacion y circularizacion generalmente es en respuesta a un estimulo tal como un estimulo mediado por una proteina. Mas particularmente, la proteina es una proteina viral o derivada de procariota o eucariota o bien una proteina regulada especifica por el desarrollo o por el tejido. El plasmido recombinante de la presente invencion es util particularmente para conferir resistencia genetica contra patogenos o inducir apoptosis u otros mecanismos de muerte celular utiles, por ejemplo, para tratar cancer o inducir esterilidad masculina o femenina en plantas. Los plasmidos recombinantes permiten, por lo tanto, expresion especifica de sitio o expresion regulada en el desarrollo de secuencias geneticas seleccionadas.
MXPA02009339A 2000-03-28 2001-03-28 Plasmido recombinante capaz de liberarse en forma circular cerrada a partir de una secuencia nucleiotidica mas grande que permite la expresion especifica de sitio o la expresion regulada en el desarrollo de secuenicas geneticas seleccionadas. MXPA02009339A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPQ6516A AUPQ651600A0 (en) 2000-03-28 2000-03-28 Suicide gene vector
AUPR1081A AUPR108100A0 (en) 2000-10-27 2000-10-27 A construct
PCT/AU2001/000349 WO2001072996A1 (en) 2000-03-28 2001-03-28 A construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences

Publications (1)

Publication Number Publication Date
MXPA02009339A true MXPA02009339A (es) 2004-05-14

Family

ID=25646287

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02009339A MXPA02009339A (es) 2000-03-28 2001-03-28 Plasmido recombinante capaz de liberarse en forma circular cerrada a partir de una secuencia nucleiotidica mas grande que permite la expresion especifica de sitio o la expresion regulada en el desarrollo de secuenicas geneticas seleccionadas.

Country Status (16)

Country Link
US (2) US7863430B2 (es)
EP (1) EP1282697B1 (es)
JP (1) JP4797141B2 (es)
KR (1) KR20030047880A (es)
CN (1) CN1426465B (es)
AP (2) AP1440A (es)
AT (1) ATE376589T1 (es)
AU (1) AU4393901A (es)
BR (1) BRPI0109522B8 (es)
CA (1) CA2404471C (es)
DE (1) DE60131075T2 (es)
ES (1) ES2296736T3 (es)
MX (1) MXPA02009339A (es)
NZ (1) NZ521488A (es)
PT (1) PT1282697E (es)
WO (1) WO2001072996A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102573514B (zh) 2007-12-03 2015-01-07 先正达参股股份有限公司 工程化改造酶易感性肌醇六磷酸酶
WO2010099320A1 (en) * 2009-02-25 2010-09-02 University Of Hawaii Plant resistance to banana bunchy top virus
CA2912665C (en) 2013-05-15 2021-03-02 Robert Kruse Intracellular translation of circular rna
GB201402249D0 (en) 2014-02-10 2014-03-26 Vela Operations Pte Ltd NGS systems control and methods involving the same
WO2017136895A1 (en) * 2016-02-10 2017-08-17 Queensland University Of Technology Constructs and methods for conferring virus resistance
WO2019236673A1 (en) 2018-06-06 2019-12-12 Massachusetts Institute Of Technology Circular rna for translation in eukaryotic cells
CN115806976A (zh) * 2019-03-20 2023-03-17 中国科学院上海营养与健康研究所 利用环形rna进行蛋白翻译及其应用
KR20220027855A (ko) 2019-05-22 2022-03-08 매사추세츠 인스티튜트 오브 테크놀로지 원형 rna 조성물 및 방법
EP4289951A3 (en) 2019-12-04 2024-03-13 Orna Therapeutics, Inc. Circular rna compositions and methods

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPN328595A0 (en) * 1995-05-31 1995-06-22 Queensland University Of Technology Intergenic regions of banana bunchy top virus
US6077992A (en) * 1997-10-24 2000-06-20 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
EP1115870A2 (en) * 1998-09-23 2001-07-18 E.I. Du Pont De Nemours And Company Binary viral expression system in plants

Also Published As

Publication number Publication date
AU4393901A (en) 2001-10-08
CN1426465B (zh) 2016-01-20
EP1282697A1 (en) 2003-02-12
CA2404471A1 (en) 2001-10-04
BR0109522A (pt) 2003-06-10
NZ521488A (en) 2004-07-30
ES2296736T3 (es) 2008-05-01
BRPI0109522B1 (pt) 2015-12-08
PT1282697E (pt) 2008-02-01
JP4797141B2 (ja) 2011-10-19
WO2001072996A1 (en) 2001-10-04
EP1282697B1 (en) 2007-10-24
US20110119782A1 (en) 2011-05-19
DE60131075D1 (de) 2007-12-06
BRPI0109522B8 (pt) 2017-04-11
JP2003528606A (ja) 2003-09-30
US8829170B2 (en) 2014-09-09
DE60131075T2 (de) 2008-07-31
US7863430B2 (en) 2011-01-04
ATE376589T1 (de) 2007-11-15
CA2404471C (en) 2010-09-28
EP1282697A4 (en) 2004-06-23
CN1426465A (zh) 2003-06-25
US20040121430A1 (en) 2004-06-24
AP1645A (en) 2006-07-28
AP1440A (en) 2005-06-28
KR20030047880A (ko) 2003-06-18
AP2002002636A0 (en) 2002-09-30

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