MX347843B - Un microorganismo para la producción de metionina con importación de glucosa mejorada. - Google Patents

Un microorganismo para la producción de metionina con importación de glucosa mejorada.

Info

Publication number
MX347843B
MX347843B MX2013015202A MX2013015202A MX347843B MX 347843 B MX347843 B MX 347843B MX 2013015202 A MX2013015202 A MX 2013015202A MX 2013015202 A MX2013015202 A MX 2013015202A MX 347843 B MX347843 B MX 347843B
Authority
MX
Mexico
Prior art keywords
methionine
glucose
microorganism
source
import
Prior art date
Application number
MX2013015202A
Other languages
English (en)
Other versions
MX2013015202A (es
Inventor
Rainer Figge
Wanda Dischert
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of MX2013015202A publication Critical patent/MX2013015202A/es
Publication of MX347843B publication Critical patent/MX347843B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se refiere a un microorganismo recombinante para la producción de metionina mejorada, caracterizado porque comprende: a) al menos una modificación para producir metionina a partir de glucosa como fuente principal de carbono mediante la fermentación; y b) al menos una modificación para mejorar la importación de glucosa, en donde la importación de glucosa se incrementa en comparación con el microorganismo sin las modificaciones especificadas mediante la sobreexpresión del gen ptsG que codifica para la enzima PTS IICBGlc y la atenuación del gen dgsA que codifica un regulador transcripcional, en donde la expresión de al menos uno de los siguientes genes se atenúa: metJ, pykA, purU, o udhA,y en donde dicho microorganismo es Escherichia coli.
MX2013015202A 2011-06-29 2012-06-29 Un microorganismo para la producción de metionina con importación de glucosa mejorada. MX347843B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161502528P 2011-06-29 2011-06-29
EP11305829 2011-06-29
PCT/EP2012/062674 WO2013001055A1 (en) 2011-06-29 2012-06-29 A microorganism for methionine production with enhanced glucose import

Publications (2)

Publication Number Publication Date
MX2013015202A MX2013015202A (es) 2014-02-17
MX347843B true MX347843B (es) 2017-05-16

Family

ID=47423448

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013015202A MX347843B (es) 2011-06-29 2012-06-29 Un microorganismo para la producción de metionina con importación de glucosa mejorada.

Country Status (12)

Country Link
US (1) US9506092B2 (es)
EP (1) EP2726626B1 (es)
JP (1) JP2014522642A (es)
KR (1) KR102029819B1 (es)
CN (1) CN103781913B (es)
AR (1) AR086790A1 (es)
BR (1) BR112013033675B1 (es)
ES (1) ES2654589T3 (es)
MX (1) MX347843B (es)
MY (1) MY166543A (es)
PL (1) PL2726626T3 (es)
WO (1) WO2013001055A1 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9506093B2 (en) 2012-06-18 2016-11-29 Metabolic Explorer Recombinant microorganism for the fermentative production of methionine
RU2678757C2 (ru) * 2013-08-30 2019-01-31 Эвоник Дегусса Гмбх Микроорганизмы для производства метионина с улучшенным выходом метионина
JP2016529899A (ja) 2013-08-30 2016-09-29 メタボリック エクスプローラー メチオニンシンターゼ活性及びメチオニン流出が改善された、メチオニン生産のための微生物
MX2017002662A (es) 2014-09-01 2017-05-30 Metabolic Explorer Sa Metodo y microorganismo para produccion de metionina por fermentacion con eflujo de metionina mejorado.
EP3050970B1 (en) 2015-01-28 2019-09-18 Metabolic Explorer Modified microorganism for optimized production of 1,4-butanediol
US10717998B2 (en) * 2015-03-18 2020-07-21 Basf Se Recombinant microorganism for improved production of fine chemicals
US10858675B2 (en) 2015-04-07 2020-12-08 Metabolic Explorer Modified microorganism for the optimized production of 2,4-dihydroxybutyrate with enhanced 2,4-dihydroxybutyrate efflux
EP3280694B1 (en) 2015-04-07 2021-11-24 Metabolic Explorer Modified microorganism for the optimized production of 2,4-dihydroxyburyrate
EP3331998B1 (en) 2015-08-07 2020-06-24 Evonik Operations GmbH Protein thiocarboxylate-dependent l-methionine production by fermentation
PL3380627T3 (pl) 2015-11-27 2020-01-31 Evonik Degussa Gmbh Sposób wytwarzania L-metioniny
WO2017118871A1 (en) 2016-01-08 2017-07-13 Metabolic Explorer Method to produce l-methionine by a fermentative production
KR101853961B1 (ko) * 2016-04-19 2018-06-20 (주)휴맥스 포스 입력의 식별 서비스 제공 장치 및 방법
EP3296404A1 (en) 2016-09-15 2018-03-21 Evonik Degussa GmbH Modified microorganism for production of methionine
EP3354742A1 (en) 2017-01-26 2018-08-01 Metabolic Explorer Methods and microorganisms for the production of glycolic acid and/or glyoxylic acid
EP3652319B1 (en) 2017-07-11 2023-10-25 Adisseo France S.A.S. Methionine-producing yeast
EP3470512A1 (en) 2017-10-10 2019-04-17 Metabolic Explorer Mutant phosphoserine aminotransferase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate
KR102221040B1 (ko) * 2019-05-09 2021-03-03 씨제이제일제당 주식회사 L-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산을 생산하는 방법
WO2023025656A1 (en) 2021-08-25 2023-03-02 Metabolic Explorer Dehydrogenase mutants and applications thereof in amino acid synthesis
WO2023089028A1 (en) 2021-11-17 2023-05-25 Metabolic Explorer Microorganism and method for the improved production of valine
WO2023166027A1 (en) 2022-03-01 2023-09-07 Metabolic Explorer Microorganism and method for the improved production of leucine and/or isoleucine
CA3225234A1 (en) 2022-08-04 2024-02-08 Metabolic Explorer Microorganism and method for the improved production of serine and/or cysteine

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4110641B2 (ja) 1998-11-17 2008-07-02 味の素株式会社 発酵法によるｌ−メチオニンの製造法
JP2000157267A (ja) 1998-11-24 2000-06-13 Ajinomoto Co Inc 変異型ｍｅｔＪ遺伝子及びＬ−メチオニンの製造法
AU2001285844A1 (en) 2000-08-02 2002-02-13 Degussa A.G. Nucleotide sequences which code for the meth gene
DE10116518A1 (de) 2001-04-03 2002-10-17 Degussa Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae
ES2318022T3 (es) * 2001-07-06 2009-05-01 Evonik Degussa Gmbh Proceso para la preparacion de l-aminoacidos usando cepas de la familia enterobacteriacease con expresion del gen ptsg incrementada.
RU2245919C2 (ru) 2002-09-06 2005-02-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Способ получения l-аминокислоты, штамм escherichia coli tdh7δ mlc::cat/pprt614-продуцент l-треонина
DE10303571A1 (de) * 2003-01-30 2004-08-12 Degussa Ag Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae
US20060270013A1 (en) 2003-02-18 2006-11-30 Michel Chateau Method for the production of evolved microorganisms which permit the generation or modification of metabolic pathways
DE10314618A1 (de) * 2003-04-01 2004-10-14 Degussa Ag Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae
DE10359594A1 (de) 2003-12-18 2005-07-28 Basf Ag PEF-TU-Expressionseinheiten
DE102004003411A1 (de) * 2004-01-23 2005-09-01 Degussa Ag Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae
DE102004005836A1 (de) * 2004-02-06 2005-09-15 Degussa Ag Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae
KR100651220B1 (ko) 2004-06-29 2006-11-29 씨제이 주식회사 Ｌ-메씨오닌 생산 균주 및 상기 균주를 이용한ｌ-메씨오닌의 생산방법
DE102004035065A1 (de) 2004-07-20 2006-02-16 Basf Ag P-ET-TS-Expressionseinheiten
ES2459617T3 (es) 2006-01-04 2014-05-12 Metabolic Explorer Procedimiento para la preparación de metionina y sus precursores homoserina o succinilhomoserina que utiliza un microorganismo
WO2007119576A1 (en) * 2006-03-23 2007-10-25 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family
WO2009043372A1 (en) * 2007-10-02 2009-04-09 Metabolic Explorer Increasing methionine yield
EP2227541B1 (en) * 2007-12-18 2013-06-19 Korea Advanced Institute of Science and Technology Recombinant microorganism having an ability of using sucrose as a carbon source
WO2010020290A1 (en) * 2008-08-22 2010-02-25 Metabolic Explorer Producing methionine without n-acetyl methionine
AR083468A1 (es) 2010-10-25 2013-02-27 Metabolic Explorer Sa Aumento de la disponibilidad de nadph para la produccion de metionina
WO2012090021A1 (en) * 2010-12-30 2012-07-05 Metabolic Explorer Recombinant microorganism for the fermentative production of methionine

Also Published As

Publication number Publication date
EP2726626A1 (en) 2014-05-07
KR20140043804A (ko) 2014-04-10
MY166543A (en) 2018-07-16
PL2726626T3 (pl) 2018-05-30
US20140134680A1 (en) 2014-05-15
ES2654589T3 (es) 2018-02-14
CN103781913B (zh) 2016-02-24
AR086790A1 (es) 2014-01-22
KR102029819B1 (ko) 2019-10-08
US9506092B2 (en) 2016-11-29
CN103781913A (zh) 2014-05-07
BR112013033675A2 (pt) 2018-06-19
BR112013033675B1 (pt) 2021-06-29
EP2726626B1 (en) 2017-11-15
WO2013001055A1 (en) 2013-01-03
JP2014522642A (ja) 2014-09-08
MX2013015202A (es) 2014-02-17

Similar Documents

Publication Publication Date Title
MX347843B (es) Un microorganismo para la producción de metionina con importación de glucosa mejorada.
MX2012006777A (es) Uso de promotores inducibles en la produccion de metionina.
MX2014015768A (es) Microorganismo recombinante para la produccion fermentativa de metionina.
MY174404A (en) Producing methionine without n-acyl-methionine
MX2009004659A (es) Procedimiento para la produccion biologica de 1,3-propanodiol a partir de glicerina con un alto rendimiento.
WO2012177619A3 (en) Microorganisms for producing 1,3-butanediol and methods related thereto
MY164623A (en) Method for producing high amount of glycolic acid by fermentation
BR112013009552A2 (pt) produção de butanol a partir de monóxido de carbono por um micro-organismo recombinante
MX2013008988A (es) Microorganismo y metodos para la biosintesis de butadieno.
WO2013076144A3 (en) Microorganism strains for the production of 2,3-butanediol
MX2016002616A (es) Microorganismo para la produccion de metionina con actividad de metionina sintasa y eflujo de metionina mejorados.
WO2013112939A3 (en) Methods for succinate production
WO2013105802A3 (ko) 자일로즈 이용능이 부여된 코리네박테리움 속 미생물 및 이를 이용한 ｌ-라이신의 생산방법
WO2011036213A3 (en) Fermentation process for producing glycolic acid
WO2011157728A8 (en) Use of inducible promoters in the production of glycolic acid
WO2012015949A3 (en) Methods and compositions for improving yields of reduced products of photosynthetic microorganisms
WO2011115439A3 (ko) 5'-크산틸산 및 5'-구아닐산 생산능이 향상된 미생물 및 이를 이용한 5'-크산틸산 또는 5'-구아닐산의 생산방법
MY194223A (en) Method and microorganism for methionine production by fermentation with improved methionine efflux
WO2014049382A3 (en) Ethylenediamine fermentative production by a recombinant microorganism
WO2011106576A3 (en) Methods and compositions for enhanced enzyme activity in fermenting microorganisms
MX2010008812A (es) Produccion aumentada de etanol por celulas bacterianas.
BR112012028202A2 (pt) Método para a produção de isopropanol e levedura recombinante capaz de produzir isoprapanol.
MY179877A (en) Method for producing 2,3-butanediol
WO2014041011A3 (en) Method for the production of primary amines
WO2013027053A3 (en) Method for enhancement of enzyme activity

Legal Events

Date Code Title Description
FG Grant or registration