WO2014049382A3 - Ethylenediamine fermentative production by a recombinant microorganism - Google Patents

Ethylenediamine fermentative production by a recombinant microorganism Download PDF

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Publication number
WO2014049382A3
WO2014049382A3 PCT/IB2012/002218 IB2012002218W WO2014049382A3 WO 2014049382 A3 WO2014049382 A3 WO 2014049382A3 IB 2012002218 W IB2012002218 W IB 2012002218W WO 2014049382 A3 WO2014049382 A3 WO 2014049382A3
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WO
WIPO (PCT)
Prior art keywords
ethylenediamine
fermentative production
recombinant microorganism
microorganism
fermentative
Prior art date
Application number
PCT/IB2012/002218
Other languages
French (fr)
Other versions
WO2014049382A2 (en
Inventor
Guillaume Letellier
Laurence Dumon-Seignovert
Rainer Figge
Original Assignee
Metabolic Explorer
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Publication date
Application filed by Metabolic Explorer filed Critical Metabolic Explorer
Priority to PCT/IB2012/002218 priority Critical patent/WO2014049382A2/en
Publication of WO2014049382A2 publication Critical patent/WO2014049382A2/en
Publication of WO2014049382A3 publication Critical patent/WO2014049382A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1014—Hydroxymethyl-, formyl-transferases (2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/03—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with oxygen as acceptor (1.2.3)
    • C12Y102/03001—Aldehyde oxidase (1.2.3.1), i.e. retinal oxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/02—Hydroxymethyl-, formyl- and related transferases (2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01001—Amino-acid N-acetyltransferase (2.3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01081—N-Acyl-D-amino-acid deacylase (3.5.1.81)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to a method for the fermentative production of ethylenediamine comprising culturing a microorganism in an appropriate culture medium comprising a simple source of carbon and of nitrogen, wherein the microorganism comprises a metabolic pathway for conversion of serine into ethylenediamine.
PCT/IB2012/002218 2012-09-26 2012-09-26 Ethylenediamine fermentative production by a recombinant microorganism WO2014049382A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/IB2012/002218 WO2014049382A2 (en) 2012-09-26 2012-09-26 Ethylenediamine fermentative production by a recombinant microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2012/002218 WO2014049382A2 (en) 2012-09-26 2012-09-26 Ethylenediamine fermentative production by a recombinant microorganism

Publications (2)

Publication Number Publication Date
WO2014049382A2 WO2014049382A2 (en) 2014-04-03
WO2014049382A3 true WO2014049382A3 (en) 2015-03-26

Family

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PCT/IB2012/002218 WO2014049382A2 (en) 2012-09-26 2012-09-26 Ethylenediamine fermentative production by a recombinant microorganism

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Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170104489A (en) 2014-12-23 2017-09-15 게노마티카 인코포레이티드 How to make and process diamines
BR112019012848B1 (en) * 2016-12-21 2024-02-20 Fertinagro Biotech, S.L. METHOD FOR OBTAINING POLYAMINES FROM A PROTEIN MATERIAL
PT3820585T (en) 2018-07-10 2023-01-12 Givaudan Sa Improvements in or relating to organic compounds
WO2020163935A1 (en) 2019-02-15 2020-08-20 Braskem S.A. Microorganisms and methods for the production of glycolic acid and glycine via reverse glyoxylate shunt
JP2022521904A (en) 2019-02-20 2022-04-13 ブラスケム エス．エー． Microorganisms and methods for the production of oxygen-containing compounds from hexose
US11584944B2 (en) 2019-02-20 2023-02-21 Braskem S.A. Degradation pathway for pentose and hexose sugars

Citations (5)

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Publication number Priority date Publication date Assignee Title
US2760979A (en) * 1952-12-06 1956-08-28 Wacker Chemie Gmbh Process for manufacturing ethylenediamine hydrochloride
WO2007144018A1 (en) * 2006-06-12 2007-12-21 Metabolic Explorer Ethanolamine production by fermentation
DE102007060705A1 (en) * 2007-12-17 2009-06-18 Evonik Degussa Gmbh ω-aminocarboxylic acids or their lactams, producing, recombinant cells
WO2009113855A2 (en) * 2008-03-11 2009-09-17 Dsm Ip Assets B.V. PREPARATION OF 6-AMINOCAPROIC ACID FROM α-KETOPIMELIC ACID
WO2011031147A1 (en) * 2009-09-11 2011-03-17 Dsm Ip Assets B.V. Preparation of a compound comprising an amine group from an alpha-keto acid

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TW313589B (en) 1991-12-12 1997-08-21 Wacker Chemie Gmbh
JP4066543B2 (en) 1998-01-12 2008-03-26 味の素株式会社 Method for producing L-serine by fermentation
JP3614746B2 (en) 2000-03-01 2005-01-26 松下電器産業株式会社 Semiconductor laser device and optical pickup device
US20060270013A1 (en) 2003-02-18 2006-11-30 Michel Chateau Method for the production of evolved microorganisms which permit the generation or modification of metabolic pathways
FR2862068B1 (en) 2003-11-06 2007-10-12 Metabolic Explorer Sa OPTIMIZED MICROORGANISM STRAINS FOR NADPH CONSUMPTION BIOSYNTHESIS PATHWAYS
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WO2005111202A1 (en) 2004-05-12 2005-11-24 Metabolic Explorer Recombinant enzyme with altered feedback sensitivity
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BRPI0607939A2 (en) 2005-02-07 2010-11-30 Metabolic Explorer Sa microorganism, and method for the fermentative preparation of an amino acid
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US2760979A (en) * 1952-12-06 1956-08-28 Wacker Chemie Gmbh Process for manufacturing ethylenediamine hydrochloride
WO2007144018A1 (en) * 2006-06-12 2007-12-21 Metabolic Explorer Ethanolamine production by fermentation
DE102007060705A1 (en) * 2007-12-17 2009-06-18 Evonik Degussa Gmbh ω-aminocarboxylic acids or their lactams, producing, recombinant cells
WO2009113855A2 (en) * 2008-03-11 2009-09-17 Dsm Ip Assets B.V. PREPARATION OF 6-AMINOCAPROIC ACID FROM α-KETOPIMELIC ACID
WO2011031147A1 (en) * 2009-09-11 2011-03-17 Dsm Ip Assets B.V. Preparation of a compound comprising an amine group from an alpha-keto acid

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