MX2016002616A - Microorganismo para la produccion de metionina con actividad de metionina sintasa y eflujo de metionina mejorados. - Google Patents

Microorganismo para la produccion de metionina con actividad de metionina sintasa y eflujo de metionina mejorados.

Info

Publication number
MX2016002616A
MX2016002616A MX2016002616A MX2016002616A MX2016002616A MX 2016002616 A MX2016002616 A MX 2016002616A MX 2016002616 A MX2016002616 A MX 2016002616A MX 2016002616 A MX2016002616 A MX 2016002616A MX 2016002616 A MX2016002616 A MX 2016002616A
Authority
MX
Mexico
Prior art keywords
methionine
efflux
microorganism
synthase activity
production
Prior art date
Application number
MX2016002616A
Other languages
English (en)
Inventor
Rainer Figge
Wanda Dischert
Perrine Vasseur
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of MX2016002616A publication Critical patent/MX2016002616A/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/12Methionine; Cysteine; Cystine
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245Escherichia (G)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1003Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007Methyltransferases (general) (2.1.1.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y201/00Transferases transferring one-carbon groups (2.1)
    • C12Y201/01Methyltransferases (2.1.1)
    • C12Y201/01013Methionine synthase (2.1.1.13)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención está relacionada a un microorganismo recombinante optimizado para la producción de metionina y/o sus derivados, en donde en dicho microorganismo recombinante, la actividad de la metionina sintasa dependiente de cobalamina y el eflujo de metionina son mejorados; la solicitud también está relacionada a un método para optimizar la producción fermentativa de metionina y/o sus derivados, que comprende los pasos de: c. cultivar un microorganismo recombinante en donde en dicho microorganismo, la actividad de la metionina sintasa dependiente de cobalamina y el eflujo de metionina son mejoradas, en un medio de cultivo apropiado que comprende una fuente de carbono fermentable y una fuente de azufre, y d. recuperar la metionina y/o sus derivados del medio de cultivo.
MX2016002616A 2013-08-30 2014-09-01 Microorganismo para la produccion de metionina con actividad de metionina sintasa y eflujo de metionina mejorados. MX2016002616A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13306185 2013-08-30
PCT/EP2014/068539 WO2015028674A1 (en) 2013-08-30 2014-09-01 Microorganism for methionine production with improved methionine synthase activity and methionine efflux

Publications (1)

Publication Number Publication Date
MX2016002616A true MX2016002616A (es) 2016-06-06

Family

ID=49162096

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016002616A MX2016002616A (es) 2013-08-30 2014-09-01 Microorganismo para la produccion de metionina con actividad de metionina sintasa y eflujo de metionina mejorados.

Country Status (12)

Country Link
US (1) US10196658B2 (es)
EP (1) EP3039153B1 (es)
JP (1) JP2016529899A (es)
KR (1) KR102270626B1 (es)
CN (1) CN105658803B (es)
BR (1) BR112016004367B1 (es)
CA (1) CA2922597A1 (es)
ES (1) ES2693789T3 (es)
MX (1) MX2016002616A (es)
PL (1) PL3039153T3 (es)
RU (1) RU2016110763A (es)
WO (1) WO2015028674A1 (es)

Families Citing this family (7)

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MX2017002662A (es) 2014-09-01 2017-05-30 Metabolic Explorer Sa Metodo y microorganismo para produccion de metionina por fermentacion con eflujo de metionina mejorado.
EP3331998B1 (en) 2015-08-07 2020-06-24 Evonik Operations GmbH Protein thiocarboxylate-dependent l-methionine production by fermentation
PL3380627T3 (pl) 2015-11-27 2020-01-31 Evonik Degussa Gmbh Sposób wytwarzania L-metioniny
WO2017118871A1 (en) 2016-01-08 2017-07-13 Metabolic Explorer Method to produce l-methionine by a fermentative production
EP3296404A1 (en) 2016-09-15 2018-03-21 Evonik Degussa GmbH Modified microorganism for production of methionine
CN109055289B (zh) * 2018-07-27 2020-10-27 浙江工业大学 一种高产l-甲硫氨酸的重组大肠杆菌及其应用
CN112961807B (zh) * 2021-03-30 2023-01-20 中国科学院成都生物研究所 一种微生物组合物及在促进青稞种子发芽和生长上的应用

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Also Published As

Publication number Publication date
WO2015028674A1 (en) 2015-03-05
KR102270626B1 (ko) 2021-06-30
RU2016110763A (ru) 2017-10-05
KR20160044037A (ko) 2016-04-22
JP2016529899A (ja) 2016-09-29
EP3039153B1 (en) 2018-08-22
US20160177352A1 (en) 2016-06-23
BR112016004367B1 (pt) 2022-06-14
CN105658803B (zh) 2020-06-23
CA2922597A1 (en) 2015-03-05
CN105658803A (zh) 2016-06-08
EP3039153A1 (en) 2016-07-06
ES2693789T3 (es) 2018-12-13
US10196658B2 (en) 2019-02-05
PL3039153T3 (pl) 2019-02-28
BR112016004367A2 (pt) 2017-10-17

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Owner name: EVONIK DEGUSSA GMBH.*

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