KR900014594A - 핵산 분리방법 - Google Patents

핵산 분리방법 Download PDF

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Publication number
KR900014594A
KR900014594A KR1019900004012A KR900004012A KR900014594A KR 900014594 A KR900014594 A KR 900014594A KR 1019900004012 A KR1019900004012 A KR 1019900004012A KR 900004012 A KR900004012 A KR 900004012A KR 900014594 A KR900014594 A KR 900014594A
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nucleic acid
solid
complex
silica particles
washed
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KR1019900004012A
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KR0148693B1 (ko
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마리아 알레이다 헨리에트
레느 부움 빌렘
키에비츠 팀
프랭클린 렌스 피터
Original Assignee
에프.쥐.엠.헤르만스, 에이.쥐.제이.베르메렌
악조 엔.브이.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Hard Magnetic Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

내용 없음

Description

핵산 분리방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (15)

  1. 출발물질, 카오트로픽 물질 및 핵산 결합 고상을 혼합하고, 자체에 핵산이 결합된 고상을 액으로부터 분리한 후, 수득된 고상-핵산 복합체를 세정하고, 이어서 필요하다면 상기 복합체로부터 핵산을 용리시키는 것을 특징으로 하는 핵산-함유 출발물질로부터 핵산을 분리하는 방법.
  2. 제1항에 있어서, 사용하는 출발물질이 전혈, 혈청, 연막뇨, 분변, 뇌척수액, 정자, 타액, 조직 및 세포 배양물과 같은 핵산-함유 생물학적 물질인 것을 특징으로 하는 방법.
  3. 제1항 또는 제2항에 있어서, 사용하는 카오트로픽 물질이 구아니디늄염, 요오드화나트륨, 요오드화칼륨, 소듐(이소)티오시아네이트, 우레아 및 이들 상호간의 조합물들로 구성된 군으로부터 선택되는 것을 특징으로 하는 방법.
  4. 제3항에 있어서, 사용하는 구아니디늄염이 구아니디늄(이소)티오시아네이트인 것을 특징으로 하는 방법.
  5. 제1항에 있어서, 사용하는 핵산-결합 고상이 실리카입자, 중합물질, 필터물질, 폴리스티렌 비이드 및 니트로셀룰로오스 종이로 구성된 군으로부터 선택되는 것을 특징으로 하는 방법.
  6. 제1항 내지 제5항중 어느 한 항에 있어서, DNA 및/또는 RNA를 분리하는 것을 특징으로 하는 방법.
  7. 제1항 내지 제6항중 어느 한 항에 있어서, 주로 0.05㎛내지500㎛범위내의 입자크기를 갖는 실리카 입자들을 사용하는 것을 특징으로 하는 방법.
  8. 제1항 내지 제6항중 어느 한 항에 있어서, 주로 0.1내지200㎛범위내의 입자크기를 갖는 실리카 입자들을 사용하는 것을 특징으로 하는 방법.
  9. 제1항 내지 제8항중 어느 한 항에 있어서, 주로 1내지200㎛범위내의 입자크기를 갖는 실리카 입자들을 사용하는 것을 특징으로 하는 방법.
  10. 제1항 내지 제9항중 어느 한 항에 있어서, 생성된 고상-핵산 복합체를 침강 및 상층액 제거처리하여 액으로부터 분리한후, 복합체를 카오트로픽 물질-함유 세정 완층액으로 세정하는 것을 특징으로 하는 방법.
  11. 제10항에 있어서, 세정 완충액으로 세정한 고상-핵산 복합체를 1종 또는 그 이상의 유기용매로 연속적으로 다시 세정한후, 건조시키는 것을 특징으로 하는 방법.
  12. 제11항에 있어서, 세정 및 건조시킨 고상-핵산 복합체내에 존재하는 핵산을 용출 완충액을 사용하여 용리시키는 것을 특징으로 하는 방법.
  13. 제1항에 있어서, 수득된 고상-핵산 복합체를 상기 고상에 결합되거나 또는 그로부터 용리된 핵산을 확장시키기 위한 목적으로 존재하는 성분들의 혼합물과 접촉시키는 것을 특징으로 하는 방법.
  14. 제1항에 기재한 방법을 수행하기 위한 장치들의 조합체.
  15. 제13항에 기재한 방법을 수행하기 위한 시험키트.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900004012A 1989-03-23 1990-03-22 핵산 분리방법 KR0148693B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL8900725A NL8900725A (nl) 1989-03-23 1989-03-23 Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
NL8900725 1989-03-23

Publications (2)

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KR900014594A true KR900014594A (ko) 1990-10-24
KR0148693B1 KR0148693B1 (ko) 1998-08-01

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EP (2) EP0819696A3 (ko)
JP (3) JP2680462B2 (ko)
KR (1) KR0148693B1 (ko)
AT (1) ATE156830T1 (ko)
AU (1) AU641641B2 (ko)
CA (2) CA2271603A1 (ko)
DE (2) DE69031237T3 (ko)
DK (1) DK0389063T4 (ko)
ES (1) ES2085245T5 (ko)
GR (2) GR960300019T1 (ko)
NL (1) NL8900725A (ko)
ZA (1) ZA902190B (ko)

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