WO1999032616A2 - Verfahren zur isolierung von kurz- und langkettigen nukleinsäuren - Google Patents
Verfahren zur isolierung von kurz- und langkettigen nukleinsäuren Download PDFInfo
- Publication number
- WO1999032616A2 WO1999032616A2 PCT/DE1998/003728 DE9803728W WO9932616A2 WO 1999032616 A2 WO1999032616 A2 WO 1999032616A2 DE 9803728 W DE9803728 W DE 9803728W WO 9932616 A2 WO9932616 A2 WO 9932616A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acids
- carrier
- membranes
- materials
- carrier material
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
Definitions
- a method for isolating nucleic acids that is practical for a large number of different applications is shown in US Pat. No. 5,234,809 (Boom).
- a method for isolating nucleic acids from nucleic acid-containing starting materials by incubating the starting material with a chaotropic buffer and a DNA-binding solid phase is described there.
- the chaotropic buffers implement both the lysis of the starting material and the binding of the nucleic acids to the solid phase.
- the method is well suited for isolating nucleic acids from small sample quantities and is particularly useful in the area of isolating viral nucleic acids.
- the remaining ethanol is finally removed from the carrier material by centrifugation at 5000 rpm for 10 min or by incubation of the centrifugation column at 37 ° C. for 10 min.
- the bound plasmid DNA is detached by adding 200 ⁇ l - 300 ⁇ l of a low salt buffer (eg 10 mm Tris-HCl) at 70 ° C. and subsequent centrifugation for 5 minutes at 5000 rpm.
- the centrifugate contains the isolated plasmid DNA.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Saccharide Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002315257A CA2315257A1 (en) | 1997-12-18 | 1998-12-18 | Method for isolating short and long-chain nucleic acids |
AU25097/99A AU2509799A (en) | 1997-12-18 | 1998-12-18 | Method for isolating short and long-chain nucleic acids |
JP2000525535A JP2001526888A (ja) | 1997-12-18 | 1998-12-18 | 短鎖および長鎖核酸の分離方法 |
EP98966787A EP1037973A2 (de) | 1997-12-18 | 1998-12-18 | Verfahren zur isolierung von kurz- und langkettigen nukleinsäuren |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19758102.1 | 1997-12-18 | ||
DE19758102 | 1997-12-18 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1999032616A2 true WO1999032616A2 (de) | 1999-07-01 |
WO1999032616A3 WO1999032616A3 (de) | 1999-09-10 |
Family
ID=7853518
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DE1998/003728 WO1999032616A2 (de) | 1997-12-18 | 1998-12-18 | Verfahren zur isolierung von kurz- und langkettigen nukleinsäuren |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1037973A2 (de) |
JP (1) | JP2001526888A (de) |
AU (1) | AU2509799A (de) |
CA (1) | CA2315257A1 (de) |
DE (1) | DE19858447A1 (de) |
WO (1) | WO1999032616A2 (de) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102008057317A1 (de) | 2007-11-13 | 2009-09-10 | Stratec Biomedical Systems Ag | Vorrichtung und Verfahren zur Aufreinigung von Biomolekülen |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7148343B2 (en) * | 2001-10-12 | 2006-12-12 | Gentra Systems, Inc. | Compositions and methods for using a solid support to purify RNA |
DE10222133A1 (de) * | 2002-05-17 | 2003-12-04 | Gl Biotech Gmbh | Verfahren zur Nukleinsäure-Extraktion und Nukleinsäure-Reinigung |
US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
KR101569832B1 (ko) * | 2008-11-19 | 2015-11-18 | 삼성전자주식회사 | 시료 중의 게놈 dna 및 플라스미드 dna를 서로 분리하는 방법 및 그를 위한 키트 |
MX347611B (es) | 2011-06-19 | 2017-05-04 | Abogen Inc | Dispositivos, soluciones y metodos para recoleccion de muestras. |
ES2880310T3 (es) | 2014-03-07 | 2021-11-24 | Dna Genotek Inc | Composición y método para estabilizar ácidos nucleicos en muestras biológicas |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0389063A2 (de) * | 1989-03-23 | 1990-09-26 | Akzo Nobel N.V. | Verfahren zur Reinigung von Nukleinsäuren |
US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
US5155018A (en) * | 1991-07-10 | 1992-10-13 | Hahnemann University | Process and kit for isolating and purifying RNA from biological sources |
WO1995006652A1 (en) * | 1993-08-30 | 1995-03-09 | Promega Corporation | Nucleic acid purification compositions and methods |
WO1995034569A1 (de) * | 1994-06-14 | 1995-12-21 | Invitek Gmbh | Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien |
DE4422040A1 (de) * | 1994-06-14 | 1995-12-21 | Invitek Gmbh | Verfahren zur Isolierung und Reinigung von PCR-Produkten |
DE4422044A1 (de) * | 1994-06-14 | 1995-12-21 | Invitek Gmbh | Verfahren zur Isolierung, Reinigung und ggf. Lagerung von Nukleinsäuren |
DE19506887A1 (de) * | 1995-02-17 | 1996-08-22 | Invitek Gmbh | Verfahren zur simultanen Isolierung von genomischer DNS und hochreiner Total RNS |
WO1997010331A1 (en) * | 1995-09-15 | 1997-03-20 | Beckman Instruments, Inc. | Method for purifying nucleic acids from homogeneous mixtures |
DE19717717A1 (de) * | 1997-04-18 | 1998-10-22 | Invitek Gmbh | Verfahren zur nichtinvasiven Erkennung bösartiger Tumoren der Lunge |
-
1998
- 1998-12-18 WO PCT/DE1998/003728 patent/WO1999032616A2/de not_active Application Discontinuation
- 1998-12-18 AU AU25097/99A patent/AU2509799A/en not_active Abandoned
- 1998-12-18 JP JP2000525535A patent/JP2001526888A/ja active Pending
- 1998-12-18 EP EP98966787A patent/EP1037973A2/de not_active Withdrawn
- 1998-12-18 DE DE19858447A patent/DE19858447A1/de not_active Withdrawn
- 1998-12-18 CA CA002315257A patent/CA2315257A1/en not_active Abandoned
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
EP0389063A2 (de) * | 1989-03-23 | 1990-09-26 | Akzo Nobel N.V. | Verfahren zur Reinigung von Nukleinsäuren |
US5155018A (en) * | 1991-07-10 | 1992-10-13 | Hahnemann University | Process and kit for isolating and purifying RNA from biological sources |
WO1995006652A1 (en) * | 1993-08-30 | 1995-03-09 | Promega Corporation | Nucleic acid purification compositions and methods |
WO1995034569A1 (de) * | 1994-06-14 | 1995-12-21 | Invitek Gmbh | Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien |
DE4422040A1 (de) * | 1994-06-14 | 1995-12-21 | Invitek Gmbh | Verfahren zur Isolierung und Reinigung von PCR-Produkten |
DE4422044A1 (de) * | 1994-06-14 | 1995-12-21 | Invitek Gmbh | Verfahren zur Isolierung, Reinigung und ggf. Lagerung von Nukleinsäuren |
DE19506887A1 (de) * | 1995-02-17 | 1996-08-22 | Invitek Gmbh | Verfahren zur simultanen Isolierung von genomischer DNS und hochreiner Total RNS |
WO1997010331A1 (en) * | 1995-09-15 | 1997-03-20 | Beckman Instruments, Inc. | Method for purifying nucleic acids from homogeneous mixtures |
DE19717717A1 (de) * | 1997-04-18 | 1998-10-22 | Invitek Gmbh | Verfahren zur nichtinvasiven Erkennung bösartiger Tumoren der Lunge |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102008057317A1 (de) | 2007-11-13 | 2009-09-10 | Stratec Biomedical Systems Ag | Vorrichtung und Verfahren zur Aufreinigung von Biomolekülen |
US8685322B2 (en) | 2007-11-13 | 2014-04-01 | Stratec Biomedical Ag | Apparatus and method for the purification of biomolecules |
Also Published As
Publication number | Publication date |
---|---|
CA2315257A1 (en) | 1999-07-01 |
EP1037973A2 (de) | 2000-09-27 |
JP2001526888A (ja) | 2001-12-25 |
WO1999032616A3 (de) | 1999-09-10 |
AU2509799A (en) | 1999-07-12 |
DE19858447A1 (de) | 1999-07-01 |
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