JPS6233880B2 - - Google Patents

Info

Publication number: JPS6233880B2
Authority: JP; Japan
Prior art keywords: bilirubin; oxidase; reaction; quantifying; direct
Prior art date: 1982-07-23
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP57127632A

Other languages

English (en)

Japanese (ja)

Other versions

JPS5917999A (ja

Inventor

Akira Kosaka

Sawao Murao

Kenichi Hirano

Noriaki Tanaka

Kunyoshi Matsunaga

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Amano Enzyme Inc

Original Assignee

Amano Pharmaceutical Co Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1982-07-23

Filing date

1982-07-23

Publication date

1987-07-23

1982-07-23 Application filed by Amano Pharmaceutical Co Ltd filed Critical Amano Pharmaceutical Co Ltd

1982-07-23 Priority to JP12763282A priority Critical patent/JPS5917999A/ja

1982-10-20 Priority to DE3249743A priority patent/DE3249743C2/de

1982-10-20 Priority to DE19823239236 priority patent/DE3239236A1/de

1982-10-21 Priority to FR8217632A priority patent/FR2521589B1/fr

1982-10-25 Priority to US06/436,385 priority patent/US4554249A/en

1982-10-25 Priority to CA000414104A priority patent/CA1185883A/en

1982-10-25 Priority to GB08230397A priority patent/GB2115926B/en

1983-06-21 Priority to IT48544/83A priority patent/IT1203658B/it

1983-07-19 Priority to ES524270A priority patent/ES8501444A1/es

1984-01-30 Publication of JPS5917999A publication Critical patent/JPS5917999A/ja

1984-07-09 Priority to ES534138A priority patent/ES534138A0/es

1984-09-26 Priority to CA000464110A priority patent/CA1218586A/en

1984-10-01 Priority to IT8422941A priority patent/IT1213222B/it

1984-12-12 Priority to FR8419005A priority patent/FR2556010B1/fr

1984-12-18 Priority to GB08431875A priority patent/GB2152215B/en

1985-06-27 Priority to US06/749,425 priority patent/US4839279A/en

1987-07-23 Publication of JPS6233880B2 publication Critical patent/JPS6233880B2/ja

Status Granted legal-status Critical Current

Links

BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 claims description 140
108010015428 Bilirubin oxidase Proteins 0.000 claims description 45
238000000034 method Methods 0.000 claims description 32
238000006243 chemical reaction Methods 0.000 claims description 30
239000003153 chemical reaction reagent Substances 0.000 claims description 18
239000000203 mixture Substances 0.000 claims description 17
238000008789 Direct Bilirubin Methods 0.000 claims description 15
238000008050 Total Bilirubin Reagent Methods 0.000 claims description 14
210000002966 serum Anatomy 0.000 claims description 9
108091005804 Peptidases Proteins 0.000 claims description 5
239000004365 Protease Substances 0.000 claims description 5
NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 5
229910052717 sulfur Inorganic materials 0.000 claims description 5
239000011593 sulfur Substances 0.000 claims description 5
239000004094 surface-active agent Substances 0.000 claims description 5
-1 aromatic carboxylic acids Chemical class 0.000 claims description 4
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
229940079593 drug Drugs 0.000 claims description 3
239000003814 drug Substances 0.000 claims description 3
102000035195 Peptidases Human genes 0.000 claims description 2
WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 2
239000003795 chemical substances by application Substances 0.000 claims description 2
241000894007 species Species 0.000 claims 1
102000004190 Enzymes Human genes 0.000 description 25
108090000790 Enzymes Proteins 0.000 description 25
230000000694 effects Effects 0.000 description 17
238000010521 absorption reaction Methods 0.000 description 15
239000000243 solution Substances 0.000 description 13
238000012360 testing method Methods 0.000 description 12
238000004440 column chromatography Methods 0.000 description 11
229920005654 Sephadex Polymers 0.000 description 9
239000012507 Sephadex™ Substances 0.000 description 9
239000000872 buffer Substances 0.000 description 9
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 8
241000894006 Bacteria Species 0.000 description 8
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 8
239000000126 substance Substances 0.000 description 8
238000005259 measurement Methods 0.000 description 7
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 6
125000000664 diazo group Chemical group [N-]=[N+]=[*] 0.000 description 6
NRHMKIHPTBHXPF-TUJRSCDTSA-M sodium cholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 NRHMKIHPTBHXPF-TUJRSCDTSA-M 0.000 description 6
239000000758 substrate Substances 0.000 description 6
241000222511 Coprinus Species 0.000 description 5
238000011088 calibration curve Methods 0.000 description 5
238000010586 diagram Methods 0.000 description 5
238000002360 preparation method Methods 0.000 description 5
GWZYPXHJIZCRAJ-UHFFFAOYSA-N Biliverdin Natural products CC1=C(C=C)C(=C/C2=NC(=Cc3[nH]c(C=C/4NC(=O)C(=C4C)C=C)c(C)c3CCC(=O)O)C(=C2C)CCC(=O)O)NC1=O GWZYPXHJIZCRAJ-UHFFFAOYSA-N 0.000 description 4
RCNSAJSGRJSBKK-NSQVQWHSSA-N Biliverdin IX Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(\C=C/2C(=C(C)C(=C/C=3C(=C(C=C)C(=O)N=3)C)/N\2)CCC(O)=O)N1 RCNSAJSGRJSBKK-NSQVQWHSSA-N 0.000 description 4
239000007864 aqueous solution Substances 0.000 description 4
QBUVFDKTZJNUPP-UHFFFAOYSA-N biliverdin-IXalpha Natural products N1C(=O)C(C)=C(C=C)C1=CC1=C(C)C(CCC(O)=O)=C(C=C2C(=C(C)C(C=C3C(=C(C=C)C(=O)N3)C)=N2)CCC(O)=O)N1 QBUVFDKTZJNUPP-UHFFFAOYSA-N 0.000 description 4
239000012521 purified sample Substances 0.000 description 4
YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 4
238000000108 ultra-filtration Methods 0.000 description 4
102100027211 Albumin Human genes 0.000 description 3
108010088751 Albumins Proteins 0.000 description 3
102000003425 Tyrosinase Human genes 0.000 description 3
108060008724 Tyrosinase Proteins 0.000 description 3
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
229910052921 ammonium sulfate Inorganic materials 0.000 description 3
235000011130 ammonium sulphate Nutrition 0.000 description 3
239000013060 biological fluid Substances 0.000 description 3
239000007795 chemical reaction product Substances 0.000 description 3
238000000502 dialysis Methods 0.000 description 3
238000005185 salting out Methods 0.000 description 3
239000012085 test solution Substances 0.000 description 3
210000002700 urine Anatomy 0.000 description 3
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical group OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 description 2
IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 2
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
102000001554 Hemoglobins Human genes 0.000 description 2
108010054147 Hemoglobins Proteins 0.000 description 2
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
102000004316 Oxidoreductases Human genes 0.000 description 2
108090000854 Oxidoreductases Proteins 0.000 description 2
241000222640 Polyporus Species 0.000 description 2
108010059712 Pronase Proteins 0.000 description 2
240000003152 Rhus chinensis Species 0.000 description 2
235000014220 Rhus chinensis Nutrition 0.000 description 2
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
244000061456 Solanum tuberosum Species 0.000 description 2
235000002595 Solanum tuberosum Nutrition 0.000 description 2
241000222355 Trametes versicolor Species 0.000 description 2
102100033220 Xanthine oxidase Human genes 0.000 description 2
108010093894 Xanthine oxidase Proteins 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
210000000941 bile Anatomy 0.000 description 2
238000001962 electrophoresis Methods 0.000 description 2
239000008103 glucose Substances 0.000 description 2
229940097043 glucuronic acid Drugs 0.000 description 2
210000004185 liver Anatomy 0.000 description 2
239000002609 medium Substances 0.000 description 2
FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 2
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Chemical compound OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
238000004445 quantitative analysis Methods 0.000 description 2
238000011160 research Methods 0.000 description 2
229960004889 salicylic acid Drugs 0.000 description 2
UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 2
JOXIMZWYDAKGHI-UHFFFAOYSA-N toluene-4-sulfonic acid Chemical compound CC1=CC=C(S(O)(=O)=O)C=C1 JOXIMZWYDAKGHI-UHFFFAOYSA-N 0.000 description 2
WXHLLJAMBQLULT-UHFFFAOYSA-N 2-[[6-[4-(2-hydroxyethyl)piperazin-1-yl]-2-methylpyrimidin-4-yl]amino]-n-(2-methyl-6-sulfanylphenyl)-1,3-thiazole-5-carboxamide;hydrate Chemical compound O.C=1C(N2CCN(CCO)CC2)=NC(C)=NC=1NC(S1)=NC=C1C(=O)NC1=C(C)C=CC=C1S WXHLLJAMBQLULT-UHFFFAOYSA-N 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
244000251953 Agaricus brunnescens Species 0.000 description 1
235000001674 Agaricus brunnescens Nutrition 0.000 description 1
241001103808 Albifimbria verrucaria Species 0.000 description 1
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235000011330 Armoracia rusticana Nutrition 0.000 description 1
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Natural products OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
UJKPHYRXOLRVJJ-MLSVHJFASA-N CC(O)C1=C(C)/C2=C/C3=N/C(=C\C4=C(CCC(O)=O)C(C)=C(N4)/C=C4\N=C(\C=C\1/N\2)C(C)=C4C(C)O)/C(CCC(O)=O)=C3C Chemical compound CC(O)C1=C(C)/C2=C/C3=N/C(=C\C4=C(CCC(O)=O)C(C)=C(N4)/C=C4\N=C(\C=C\1/N\2)C(C)=C4C(C)O)/C(CCC(O)=O)=C3C UJKPHYRXOLRVJJ-MLSVHJFASA-N 0.000 description 1
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240000008067 Cucumis sativus Species 0.000 description 1
235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
102000018832 Cytochromes Human genes 0.000 description 1
108010052832 Cytochromes Proteins 0.000 description 1
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GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
240000001194 Heliotropium europaeum Species 0.000 description 1
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DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
108010029541 Laccase Proteins 0.000 description 1
241000223251 Myrothecium Species 0.000 description 1
102000003992 Peroxidases Human genes 0.000 description 1
235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
235000011613 Pinus brutia Nutrition 0.000 description 1
241000018646 Pinus brutia Species 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
230000002159 abnormal effect Effects 0.000 description 1
239000000654 additive Substances 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
229960005070 ascorbic acid Drugs 0.000 description 1
235000010323 ascorbic acid Nutrition 0.000 description 1
239000011668 ascorbic acid Substances 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
238000003556 assay Methods 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
239000003833 bile salt Substances 0.000 description 1
229940093761 bile salts Drugs 0.000 description 1
BPYKTIZUTYGOLE-UHFFFAOYSA-N billirubin-IXalpha Natural products N1C(=O)C(C)=C(C=C)C1=CC1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(C=C3C(=C(C=C)C(=O)N3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-UHFFFAOYSA-N 0.000 description 1
230000008033 biological extinction Effects 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
239000008280 blood Substances 0.000 description 1
210000004556 brain Anatomy 0.000 description 1
229960001927 cetylpyridinium chloride Drugs 0.000 description 1
YMKDRGPMQRFJGP-UHFFFAOYSA-M cetylpyridinium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 YMKDRGPMQRFJGP-UHFFFAOYSA-M 0.000 description 1
229930002875 chlorophyll Natural products 0.000 description 1
235000019804 chlorophyll Nutrition 0.000 description 1
ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
229940099898 chlorophyllin Drugs 0.000 description 1
235000019805 chlorophyllin Nutrition 0.000 description 1
AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 1
YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 1
235000014113 dietary fatty acids Nutrition 0.000 description 1
229910001882 dioxygen Inorganic materials 0.000 description 1
MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 1
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BTIJJDXEELBZFS-QDUVMHSLSA-K hemin Chemical compound CC1=C(CCC(O)=O)C(C=C2C(CCC(O)=O)=C(C)\C(N2[Fe](Cl)N23)=C\4)=N\C1=C/C2=C(C)C(C=C)=C3\C=C/1C(C)=C(C=C)C/4=N\1 BTIJJDXEELBZFS-QDUVMHSLSA-K 0.000 description 1
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229910021645 metal ion Inorganic materials 0.000 description 1
235000013336 milk Nutrition 0.000 description 1
239000008267 milk Substances 0.000 description 1
210000004080 milk Anatomy 0.000 description 1
229920000847 nonoxynol Polymers 0.000 description 1
XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
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229920002401 polyacrylamide Polymers 0.000 description 1
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NNFCIKHAZHQZJG-UHFFFAOYSA-N potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 description 1
238000012545 processing Methods 0.000 description 1
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230000035945 sensitivity Effects 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229940083575 sodium dodecyl sulfate Drugs 0.000 description 1
239000003381 stabilizer Substances 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
SKIVFJLNDNKQPD-UHFFFAOYSA-N sulfacetamide Chemical compound CC(=O)NS(=O)(=O)C1=CC=C(N)C=C1 SKIVFJLNDNKQPD-UHFFFAOYSA-N 0.000 description 1
229960002673 sulfacetamide Drugs 0.000 description 1
KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
229960003495 thiamine Drugs 0.000 description 1
235000019157 thiamine Nutrition 0.000 description 1
239000011721 thiamine Substances 0.000 description 1
239000011715 vitamin B12 Substances 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Landscapes

Investigating Or Analysing Biological Materials (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

JP12763282A 1982-02-18 1982-07-23 ビリルビンの定量法及びその定量用試薬組成物 Granted JPS5917999A (ja)

Priority Applications (15)

Application Number	Priority Date	Filing Date	Title
JP12763282A JPS5917999A (ja)	1982-07-23	1982-07-23	ビリルビンの定量法及びその定量用試薬組成物
DE3249743A DE3249743C2 (it)	1982-02-18	1982-10-20
DE19823239236 DE3239236A1 (de)	1982-02-18	1982-10-20	Verfahren zur quantitativen bestimmung von physiologischen komponenten in biologischen fluessigkeiten oder gasen
FR8217632A FR2521589B1 (fr)	1982-02-18	1982-10-21	Procede pour la determination quantitative de composants physiologiques dans des fluides biologiques
US06/436,385 US4554249A (en)	1982-02-18	1982-10-25	Method for the quantitative determination of physiological components in biological fluids
CA000414104A CA1185883A (en)	1982-02-18	1982-10-25	Method for the quantitative determination of bilirubin in biological fluids
GB08230397A GB2115926B (en)	1982-02-18	1982-10-25	Method for the quantitative determination of physiological components in biological fluids
IT48544/83A IT1203658B (it)	1982-07-23	1983-06-21	Metodo per la determinazione quantitativa di componenti fisiologici in fluidi biologici
ES524270A ES8501444A1 (es)	1982-07-23	1983-07-19	Un metodo para la determinacion cuantitativa de bilirrubina conjugada en fluidos biologicos.
ES534138A ES534138A0 (es)	1982-07-23	1984-07-09	Un metodo para la determinacion cuantitativa de componentes fisiologicos en fluidos biologicos.
CA000464110A CA1218586A (en)	1982-02-18	1984-09-26	Method for the quantitative determination of physiological components in biological fluids
IT8422941A IT1213222B (it)	1982-07-23	1984-10-01	Metodo per la determinazione quantitativa di componenti fisiologici in fluidi biologici.
FR8419005A FR2556010B1 (fr)	1982-02-18	1984-12-12	Procede pour la determination quantitative de composants physiologiques dans des fluides biologiques
GB08431875A GB2152215B (en)	1982-02-18	1984-12-18	Method for the quantitative determination of physiological components in biological fluids
US06/749,425 US4839279A (en)	1982-02-18	1985-06-27	Method for the quantitative determination of physiological components in biological fluids

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP12763282A JPS5917999A (ja)	1982-07-23	1982-07-23	ビリルビンの定量法及びその定量用試薬組成物

Related Child Applications (1)

Application Number	Title	Priority Date	Filing Date
JP9993987A Division JPS62282598A (ja)	1987-04-24	1987-04-24	総ビリルビンの定量法及び定量用試薬組成物

Publications (2)

Publication Number	Publication Date
JPS5917999A JPS5917999A (ja)	1984-01-30
JPS6233880B2 true JPS6233880B2 (it)	1987-07-23

Family

ID=14964889

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP12763282A Granted JPS5917999A (ja)	1982-02-18	1982-07-23	ビリルビンの定量法及びその定量用試薬組成物

Country Status (2)

Country	Link
JP (1)	JPS5917999A (it)
IT (1)	IT1213222B (it)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS6450880U (it) *	1987-09-26	1989-03-29

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS60151561A (ja) *	1984-01-19	1985-08-09	Nitsusui Seiyaku Kk	ビリルビンの定量方法
JPS60154162A (ja) *	1984-01-24	1985-08-13	Nitsusui Seiyaku Kk	遊離ビリルビンの定量方法
JPS60178360A (ja) *	1984-02-24	1985-09-12	Fujimoto Daiagunosuteitsukusu:Kk	総ビリルビンおよび直接ビリルビンの分別定量法
KR970022316A (ko)	1995-10-27	1997-05-28	후루야 아끼라	빌리루빈의 정량 방법
CN111455018B (zh) *	2020-03-03	2023-05-23	天津大学	含有枯草芽孢杆菌漆酶的直接胆红素检测试剂盒

Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS54151193A (en) *	1978-05-19	1979-11-28	Eastman Kodak Co	Bilirubin specific bacterial enzyme composition * use and production thereof
JPS5876100A (ja) *	1981-10-30	1983-05-09	Ono Pharmaceut Co Ltd	ラツカ−ゼの使用法

1982
- 1982-07-23 JP JP12763282A patent/JPS5917999A/ja active Granted
1984
- 1984-10-01 IT IT8422941A patent/IT1213222B/it active

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS54151193A (en) *	1978-05-19	1979-11-28	Eastman Kodak Co	Bilirubin specific bacterial enzyme composition * use and production thereof
JPS5876100A (ja) *	1981-10-30	1983-05-09	Ono Pharmaceut Co Ltd	ラツカ−ゼの使用法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS6450880U (it) *	1987-09-26	1989-03-29

Also Published As

Publication number	Publication date
IT8422941A0 (it)	1984-10-01
JPS5917999A (ja)	1984-01-30
IT1213222B (it)	1989-12-14

Publication	Publication Date	Title
Poulson et al.	1975	The enzymic conversion of protoporphyrinogen IX to protoporphyrin IX. Protoporphyrinogen oxidase activity in mitochondrial extracts of Saccharomyces cerevisiae.
Kakutani et al.	1981	A blue protein as an inactivating factor for nitrite reductase from Alcaligenes faecalis strain S-6
US5432274A (en)	1995-07-11	Redox dye and method of preparation thereof using 2-hydroxypropyl-β-cyclodextrin and 1,1'-dimethylferrocene
JP3157622B2 (ja)	2001-04-16	フルクトシルアミンデグリカーゼ、その製造法及び該酵素を用いたアマドリ化合物の定量方法
US4839279A (en)	1989-06-13	Method for the quantitative determination of physiological components in biological fluids
JPH11504808A (ja)	1999-05-11	糖化タンパク質の測定
Kasidas et al.	1979	A new enzymatic method for the determination of glycollate in urine and plasma
KR880000753B1 (ko)	1988-05-04	신규의 빌리루빈 산화효소를 포함하는 시약조성물 및 그의 사용방법
EP0012446A1 (en)	1980-06-25	Acidic uricase, its production and its use for the determination of uric acid
JPS6233880B2 (it)	1987-07-23
Bognar et al.	1978	An induced aliphatic aldehyde dehydrogenase from the bioluminescent bacterium, Beneckea harveyi. Purification and properties.
JPS62282598A (ja)	1987-12-08	総ビリルビンの定量法及び定量用試薬組成物
Wood et al.	1978	The relation of the pH and concentration-dependent dissociation of porcine heart mitochondrial malate dehydrogenase
JP3416540B2 (ja)	2003-06-16	フルクトシルアミノ酸オキシダーゼ及びその製造方法
JP3087891B2 (ja)	2000-09-11	電解質測定用試薬組成物
US5100795A (en)	1992-03-31	Bile acid sulfate sulfatase, process for its preparation and method for assaying bile acid
JP2872983B2 (ja)	1999-03-24	１，５−アンヒドログルシトールの定量法及び定量用試薬
US4927753A (en)	1990-05-22	Quantitative determination of L-fucose
JP3541677B2 (ja)	2004-07-14	直接型ビリルビンの測定方法および測定用試薬
Borneleit et al.	1983	Purification and properties of the membrane-bound NADH dehydrogenase from hydrocarbon-grown Acinetobacter calcoaceticus
JP3819094B2 (ja)	2006-09-06	１，５−アンヒドログルシトール脱水素酵素、その製造法及びこれを用いる１，５−アンヒドログルシトールの定量法
Shinagawa et al.	1982	[22] d-Sorbitol dehydrogenase from Gluconobacter su☐ ydans, membrane-bound
JP2647689B2 (ja)	1997-08-27	新規アセチルポリアミンアミドヒドロラーゼ
JPH0329400B2 (it)	1991-04-24
CA1218586A (en)	1987-03-03	Method for the quantitative determination of physiological components in biological fluids

JPS6233880B2 - - Google Patents

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Priority Applications (15)

Applications Claiming Priority (1)

Related Child Applications (1)

Publications (2)

Family

ID=14964889

Family Applications (1)

Country Status (2)

Cited By (1)

Families Citing this family (5)

Citations (2)

Patent Citations (2)

Cited By (1)

Also Published As

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