DK2702153T3 - Polypeptider med endoglucanaseaktivitet og polynukleotider, som koder for dem - Google Patents
Polypeptider med endoglucanaseaktivitet og polynukleotider, som koder for dem Download PDFInfo
- Publication number
- DK2702153T3 DK2702153T3 DK12723981.2T DK12723981T DK2702153T3 DK 2702153 T3 DK2702153 T3 DK 2702153T3 DK 12723981 T DK12723981 T DK 12723981T DK 2702153 T3 DK2702153 T3 DK 2702153T3
- Authority
- DK
- Denmark
- Prior art keywords
- polypeptide
- enzyme
- acid
- endoglucanase
- fermentation
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (8)
1. isoleret polypeptid med endoglucanaseaktivitet, som er valgt fra gruppen bestående af et polypeptid, der udviser mindst 90% sekvensidentitet med aminosyrerne 18 til 419 ifølge SEQ ID NO: 2.
2. Polypeptid ifølge krav 1, som omfatter eller består af aminosyrerne 18 til 419 ifølge SEQ ID NO: 2.
3. Sammensætning, som omfatter polypeptidet ifølge et hvilket som helst af kravene 1-2. 4. isoleret polynukleotid, som koder for polypeptidet ifølge et hvilket som helst af kravene 1-2.
5. Nukleinsyrekonstruktion eller ekspressionsvektor, som omfatter poiynukieotidet ifølge krav 4, hvilket polynukleotid er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet i en ekspressionsvært.
8. Rekombinant værtscelle, som omfatter poiynukieotidet ifølge krav 4, hvilket polynukleotid er operativt forbundet til en eiier flere fremmede kontrolsekvenser, der styrer fremstillingen af polypeptidet.
7. Fremgangsmåde til fremstilling af et polypeptid med endoglucanaseaktivitet, hvilken fremgangsmåde omfatter: (a) dyrkning af værtscellen ifølge krav 6 under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
8. Transgen plante, plantedel eller plantecelle, som omfatter et polynukleotid, hvilket polynukleotid koder for polypeptidet ifølge et hvilket som helst af kravene 1-2.
9. Fremgangsmåde til nedbrydning eller omdannelse af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det celluloseholdige materiale med en enzymsammensætning ifølge krav 3.
10. Fremgangsmåde ifølge krav 9, hvor enzymsammensætningen omfatter et eller flere enzymer valgt fra gruppen bestående afen cellulase, et GH61-polypeptid med cellulolyseforbedrende aktivitet, en hemiceliulase, et expansin, en esterase, en iaccase, et iigninoiytisk enzym, en pectinase, en peroxidas /voilenin.
11. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: (a) forsukring af et ceilulosehoidigt materiale med en enzymsammensætning ifølge krav 3; (b) fermentering af det forsukrede celluloseboldige materiale med en eller flere fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161480245P | 2011-04-28 | 2011-04-28 | |
PCT/US2012/035257 WO2012149192A1 (en) | 2011-04-28 | 2012-04-26 | Polypeptides having endoglucanase activity and polynucleotides encoding same |
Publications (1)
Publication Number | Publication Date |
---|---|
DK2702153T3 true DK2702153T3 (da) | 2019-03-18 |
Family
ID=46172886
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK12723981.2T DK2702153T3 (da) | 2011-04-28 | 2012-04-26 | Polypeptider med endoglucanaseaktivitet og polynukleotider, som koder for dem |
Country Status (8)
Country | Link |
---|---|
US (2) | US9926547B2 (da) |
EP (1) | EP2702153B1 (da) |
CN (1) | CN103649308A (da) |
BR (1) | BR112013027333A2 (da) |
CA (1) | CA2834513A1 (da) |
DK (1) | DK2702153T3 (da) |
MX (1) | MX2013012325A (da) |
WO (1) | WO2012149192A1 (da) |
Families Citing this family (8)
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FR3013731B1 (fr) | 2013-11-22 | 2017-09-01 | Ifp Energies Now | Variants d'endoglucanases a activite amelioree et leurs utilisations |
FR3022558B1 (fr) | 2014-06-20 | 2019-01-25 | Proteus | Variants d'exoglucanases a activite amelioree et leurs utilisations |
FR3022557B1 (fr) | 2014-06-20 | 2019-01-25 | Proteus | Variants d'exoglucanases a activite amelioree et leurs utilisations |
BR122021020404B1 (pt) | 2016-02-19 | 2022-09-20 | Intercontinental Great Brands Llc | Processo para criar múltiplas correntes de valor a partir de fontes de biomassa |
JP7106526B2 (ja) | 2016-09-16 | 2022-07-26 | ノボザイムス アクティーゼルスカブ | 繊維洗浄方法およびシステム |
EP3658610B1 (en) | 2017-07-24 | 2023-10-25 | Novozymes A/S | Gh5 and gh30 in wet milling |
CN112204137B (zh) * | 2018-04-19 | 2024-05-14 | 诺维信公司 | 稳定化的纤维素酶变体 |
US11661592B2 (en) * | 2018-04-19 | 2023-05-30 | Novozymes A/S | Stabilized endoglucanase variants |
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- 2012-04-26 US US14/112,022 patent/US9926547B2/en not_active Expired - Fee Related
- 2012-04-26 WO PCT/US2012/035257 patent/WO2012149192A1/en active Application Filing
- 2012-04-26 EP EP12723981.2A patent/EP2702153B1/en not_active Not-in-force
- 2012-04-26 CN CN201280032735.1A patent/CN103649308A/zh active Pending
- 2012-04-26 MX MX2013012325A patent/MX2013012325A/es unknown
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- 2012-04-26 DK DK12723981.2T patent/DK2702153T3/da active
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US20180179505A1 (en) | 2018-06-28 |
MX2013012325A (es) | 2013-11-01 |
EP2702153B1 (en) | 2018-12-12 |
CN103649308A (zh) | 2014-03-19 |
US20140150137A1 (en) | 2014-05-29 |
US9926547B2 (en) | 2018-03-27 |
EP2702153A1 (en) | 2014-03-05 |
BR112013027333A2 (pt) | 2016-11-29 |
WO2012149192A1 (en) | 2012-11-01 |
CA2834513A1 (en) | 2012-11-01 |
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