DK1478755T3 - Ekspressionskloningsmetoder i trådformede svampe - Google Patents

Ekspressionskloningsmetoder i trådformede svampe

Info

Publication number
DK1478755T3
DK1478755T3 DK03704338T DK03704338T DK1478755T3 DK 1478755 T3 DK1478755 T3 DK 1478755T3 DK 03704338 T DK03704338 T DK 03704338T DK 03704338 T DK03704338 T DK 03704338T DK 1478755 T3 DK1478755 T3 DK 1478755T3
Authority
DK
Denmark
Prior art keywords
expression cloning
polypeptide
filamentous fungi
cloning methods
library
Prior art date
Application number
DK03704338T
Other languages
English (en)
Inventor
Mary Ann Stringer
Kirk Schnorr
Jesper Vind
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK1478755T3 publication Critical patent/DK1478755T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Preliminary Treatment Of Fibers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK03704338T 2002-02-19 2003-02-18 Ekspressionskloningsmetoder i trådformede svampe DK1478755T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200200256 2002-02-19
PCT/DK2003/000106 WO2003070956A1 (en) 2002-02-19 2003-02-18 Expression cloning methods in filamentous fungi

Publications (1)

Publication Number Publication Date
DK1478755T3 true DK1478755T3 (da) 2009-09-14

Family

ID=27741065

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03704338T DK1478755T3 (da) 2002-02-19 2003-02-18 Ekspressionskloningsmetoder i trådformede svampe

Country Status (7)

Country Link
US (2) US20050170350A1 (da)
EP (1) EP1478755B1 (da)
AT (1) ATE432355T1 (da)
AU (1) AU2003206683A1 (da)
DE (1) DE60327740D1 (da)
DK (1) DK1478755T3 (da)
WO (1) WO2003070956A1 (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1922314B (zh) 2004-02-25 2010-05-05 诺维信公司 真菌细胞壁降解酶
WO2008138835A1 (en) 2007-05-09 2008-11-20 Novozymes A/S Expression cloning method suitable for selecting library clones producing a polypeptide of interest
CN101970658B (zh) 2008-02-18 2014-02-26 明治制果药业株式会社 耐热性过氧化氢酶
EP3222716B1 (en) 2009-11-06 2020-08-19 Novozymes, Inc. Composition for saccharification of cellulosic material
EP2683830A1 (en) 2011-03-09 2014-01-15 Novozymes A/S Methods of increasing the cellulolytic enhancing activity of a polypeptide
WO2012122477A1 (en) 2011-03-10 2012-09-13 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
MX2013012325A (es) 2011-04-28 2013-11-01 Novozymes Inc Polipeptidos que tienen actividad de endoglucanasa y polinucleotidos que codifican los mismos.
MX2013011827A (es) 2011-04-29 2014-01-08 Novozymes Inc Metodos para mejorar la degradacion o conversion de material celulosico.
EP3342860A1 (en) 2011-11-22 2018-07-04 Novozymes, Inc. Polypeptides having beta-xylosidase activity and polynucleotides encoding same
ES2631605T3 (es) 2011-11-25 2017-09-01 Novozymes A/S Polipéptidos con actividad de lisozima y polinucleótidos que codifican los mismos
EP2929028A1 (en) 2012-12-07 2015-10-14 Novozymes A/S Method for generating site-specific mutations in filamentous fungi
CN105164254B (zh) 2013-03-08 2019-06-28 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
EP2994529B1 (en) 2013-05-10 2018-11-21 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
BR112017004251A2 (pt) 2014-09-05 2017-12-12 Novozymes As variantes de módulos de ligação a carboidrato e polinucleotídeos que os codificam
DK3262165T3 (da) 2015-02-24 2020-08-24 Novozymes As Cellobiohydrolasevarianter og polynukleotider, som koder for dem
WO2018085370A1 (en) 2016-11-02 2018-05-11 Novozymes A/S Processes for reducing production of primeverose during enzymatic saccharification of lignocellulosic material
AU2018319349B2 (en) 2017-08-25 2024-02-29 Novozymes A/S Enzyme assisted crude palm oil extraction
BR112021011384A2 (pt) 2018-12-12 2022-05-17 Novozymes As Polipeptídeo isolado, composição de enzimas, formulação de caldo inteiro ou composição de cultura de células, polinucleotídeo isolado, célula hospedeira recombinante, método de produção de um polipeptídeo, planta, parte da planta ou célula da planta transgênica, e, processos para degradação de um material celulósico ou hemicelulósico, para produção de um produto de fermentação e de fermentação de um material celulósico ou hemicelulósico
CN114958838A (zh) * 2021-02-23 2022-08-30 中国科学院天津工业生物技术研究所 一组高强度的酿酒酵母人工小启动子
CN114875046B (zh) * 2022-05-30 2023-07-18 福州大学 一种丝状真菌复制子
CN114958899A (zh) * 2022-05-30 2022-08-30 福州大学 一种能在黑曲霉细胞内自主复制的复制子

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2703598B2 (ja) * 1987-09-04 1998-01-26 ノボ―ノルディスク アクティーゼルスカブ アスペルギルス菌中でのタンパク質生産物の生産方法およびアスペルギルス菌中で使用するためのプロモーター
US5648267A (en) * 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
US6107477A (en) * 1996-09-26 2000-08-22 Aurora Biosciences Corporation Non-optimal Kozaks sequences
ATE332968T1 (de) * 1998-10-26 2006-08-15 Novozymes As Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
AU2001234443A1 (en) * 2000-01-13 2001-07-24 Novozymes Biotech, Inc. Methods for producing a polypeptide using a crippled translational initiator sequence
GB0001401D0 (en) * 2000-01-22 2000-03-08 Univ Edinburgh Methods for amplifying genetic material and use thereof

Also Published As

Publication number Publication date
WO2003070956A1 (en) 2003-08-28
EP1478755B1 (en) 2009-05-27
AU2003206683A1 (en) 2003-09-09
EP1478755A1 (en) 2004-11-24
US20050170350A1 (en) 2005-08-04
ATE432355T1 (de) 2009-06-15
US20110059862A1 (en) 2011-03-10
DE60327740D1 (de) 2009-07-09

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