DK2483403T3 - Polypeptider med xylanaseaktivitet og polynukleotider, som koder for dem - Google Patents

Polypeptider med xylanaseaktivitet og polynukleotider, som koder for dem Download PDF

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DK2483403T3
DK2483403T3 DK10763939.5T DK10763939T DK2483403T3 DK 2483403 T3 DK2483403 T3 DK 2483403T3 DK 10763939 T DK10763939 T DK 10763939T DK 2483403 T3 DK2483403 T3 DK 2483403T3
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polypeptide
vol
preferred aspect
xylan
fermentation
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DK10763939.5T
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Lan Tang
Ye Liu
Junxin Duan
Hanshu Ding
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Novozymes Inc
Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (18)

1. Isoleret polypeptid med xylanaseaktivitet, som er valgt blandt et polypeptid, der omfatter en aminosyresekvens, som udviser mindst 99% identitet med det modne polypeptid ifølge SEQ ID NO: 2.
2. Polypeptid ifølge krav 1, som omfatter eller består af aminosyresekvensen ifølge SEQ ID NO: 2 eller det modne polypeptid ifølge SEQ ID NO: 2; eller et fragment deraf med xylanaseaktivitet.
3. Polypeptid ifølge krav 1, som kodes for af polynukleotidet indeholdt i plasmid pGEM-T-Ppin3, hvilket plasmid er indeholdt i E. coli DSM 22922.
4. Isoleret polynukleotid, som omfatter en nukleotidsekvens, der koder for polypeptidet ifølge et hvilket som helst af kravene 1-3.
5. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-3, hvilken fremgangsmåde omfatter: (a) dyrkning af en celle, som i sin vildtypeform fremstiller polypeptidet, under betingelser, der er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
6. Fremgangsmåde til fremstilling af et polypeptid med xylanaseaktivitet, hvilken fremgangsmåde omfatter: (a) dyrkning af en rekombinant værtscelle, som omfatter polynukleotidet ifølge krav 4, under betingelser, der er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
7. Fremgangsmåde til nedbrydning eller omdannelse af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det celluloseholdige materiale med en enzymsammensætning i nærværelse af polypeptidet med xylanaseaktivitet ifølge et hvilket som helst af kravene 1-3.
8. Fremgangsmåde ifølge krav 7, som endvidere omfatter indvinding af det nedbrudte celluloseholdige materiale.
9. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: (a) forsukring af et celluloseholdigt materiale med en enzymsammensætning i nærværelse af polypeptidet med xylanaseaktivitet ifølge et hvilket som helst af kravene 1-3; (b) fermentering af det forsukrede celluloseholdige materiale med en eller flere fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
10. Fremgangsmåde til fermentering af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: fermentering af det celluloseholdige materiale med en eller flere fermenterende mikroorganismer, hvor det celluloseholdige materiale er forsukret med en enzymsammensætning i nærværelse af polypeptidet med xylanaseaktivitet ifølge et hvilket som helst af kravene 1-3.
11. Fremgangsmåde ifølge krav 10, hvor fermenteringen af det celluloseholdige materiale frembringer et fermenteringsprodukt.
12. Fremgangsmåde ifølge krav 11, som endvidere omfatter indvinding af fermenteringsproduktet fra fermenteringen.
13. Fremgangsmåde til nedbrydning eller omdannelse af et xylanholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det hemicelluloseholdige materiale med en enzymsammensætning i nærværelse af polypeptidet med xylanaseaktivitet ifølge et hvilket som helst af kravene 1-3.
14. Fremgangsmåde ifølge krav 13, som endvidere omfatter indvinding af det nedbrudte hemicelluloseholdige materiale.
15. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: (a) forsukring af et xylanholdigt materiale med en enzymsammensætning i nærværelse af polypeptidet med xylanaseaktivitet ifølge et hvilket som helst af kravene 1-3; (b) fermentering af det forsukrede xylanholdige materiale med en eller flere fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
16. Fremgangsmåde til fermentering af et xylanholdigt materiale, hvilken fremgangsmåde omfatter: fermentering af det xylanholdige materiale med en eller flere fermenterende mikroorganismer, hvor det hemicelluloseholdige materiale er forsukret med en enzymsammensætning i nærværelse af polypeptidet med xylanaseaktivitet ifølge et hvilket som helst af kravene 1-3.
17. Fremgangsmåde ifølge krav 16, hvor fermenteringen af det xylanholdige materiale frembringer et fermenteringsprodukt.
18. Fremgangsmåde ifølge krav 16, som endvidere omfatter indvinding af fermenteringsproduktet fra fermenteringen.
DK10763939.5T 2009-09-29 2010-09-29 Polypeptider med xylanaseaktivitet og polynukleotider, som koder for dem DK2483403T3 (da)

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US24688709P 2009-09-29 2009-09-29
PCT/US2010/050709 WO2011041405A1 (en) 2009-09-29 2010-09-29 Polypeptides having xylanase activity and polynucleotides encoding same

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US (3) US8211665B2 (da)
EP (1) EP2483403B1 (da)
CN (2) CN107338233A (da)
BR (1) BR112012006978B1 (da)
CA (1) CA2775347A1 (da)
DK (1) DK2483403T3 (da)
WO (1) WO2011041405A1 (da)

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