DE69333304D1 - Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen - Google Patents

Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen

Info

Publication number
DE69333304D1
DE69333304D1 DE69333304T DE69333304T DE69333304D1 DE 69333304 D1 DE69333304 D1 DE 69333304D1 DE 69333304 T DE69333304 T DE 69333304T DE 69333304 T DE69333304 T DE 69333304T DE 69333304 D1 DE69333304 D1 DE 69333304D1
Authority
DE
Germany
Prior art keywords
eukaryotic cells
secreted proteins
dna sequence
recombinant eukaryotic
increased production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69333304T
Other languages
English (en)
Other versions
DE69333304T2 (de
Inventor
Sirkka Keraenen
Markku Aalto
Mika Outola
Hans Ronne
Merja Penttilae
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Valtion Teknillinen Tutkimuskeskus
Original Assignee
Valtion Teknillinen Tutkimuskeskus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valtion Teknillinen Tutkimuskeskus filed Critical Valtion Teknillinen Tutkimuskeskus
Application granted granted Critical
Publication of DE69333304D1 publication Critical patent/DE69333304D1/de
Publication of DE69333304T2 publication Critical patent/DE69333304T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Seasonings (AREA)
DE69333304T 1992-10-06 1993-10-06 Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen Expired - Lifetime DE69333304T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI924494 1992-10-06
FI924494A FI924494A0 (fi) 1992-10-06 1992-10-06 Oekad produktion av avsoendrarde proteiner i eukaryotiska rekombinantceller
PCT/FI1993/000402 WO1994008024A1 (en) 1992-10-06 1993-10-06 Increased production of secreted proteins by recombinant eukaryotic cells

Publications (2)

Publication Number Publication Date
DE69333304D1 true DE69333304D1 (de) 2003-12-24
DE69333304T2 DE69333304T2 (de) 2004-08-26

Family

ID=8535988

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69333304T Expired - Lifetime DE69333304T2 (de) 1992-10-06 1993-10-06 Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen

Country Status (14)

Country Link
US (1) US5789193A (de)
EP (1) EP0665890B1 (de)
JP (1) JP3638599B2 (de)
AT (1) ATE254663T1 (de)
AU (1) AU678402B2 (de)
CA (1) CA2146240C (de)
DE (1) DE69333304T2 (de)
DK (1) DK0665890T3 (de)
EE (1) EE03074B1 (de)
ES (1) ES2211870T3 (de)
FI (2) FI924494A0 (de)
NO (1) NO319132B1 (de)
NZ (1) NZ256425A (de)
WO (1) WO1994008024A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922561A (en) * 1994-10-03 1999-07-13 Novo Nordisk Biotech, Inc. Genes encoding signal recognition particle of Aspergillus niger
DE19920712A1 (de) * 1999-05-05 2000-11-09 Rhein Biotech Proz & Prod Gmbh Verfahren zum Herstellen eines rekombinanten Proteines
US7678570B2 (en) 2004-03-01 2010-03-16 Kitakyushu Foundation For The Advancement Of Industry, Science And Technology Human cell strains for protein production, provided by selecting strains with high intracellular protein and mutating with carcinogens
US20090247609A1 (en) * 2007-12-20 2009-10-01 Hitto Kaufmann Sm-protein based secretion engineering
EP3020273B1 (de) * 2014-11-17 2018-07-04 Competence Centre on Health Technologies Verfahren zur herstellung biotechnologischer arzneimittel unter verwendung transgener rinder
EP3784791A1 (de) 2018-04-23 2021-03-03 DuPont Nutrition Biosciences ApS Erhöhung des exports von 2' fucosyllactose aus mikrobiellen zellen durch expression einer heterologen nukleinsäure
EP4194560A1 (de) 2021-12-08 2023-06-14 European Molecular Biology Laboratory Verbesserte produktion von sekretierten proteinen in pilzzellen
CN114717124B (zh) * 2022-04-19 2024-07-12 天津科技大学 一株高产麦角甾醇的酿酒酵母工程菌株、构建方法及应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0284044B1 (de) * 1987-03-23 1994-03-23 Zymogenetics, Inc. Hohe Proteinsyntheserate in Hefe

Also Published As

Publication number Publication date
US5789193A (en) 1998-08-04
DE69333304T2 (de) 2004-08-26
NO951315L (no) 1995-06-06
EP0665890B1 (de) 2003-11-19
NO319132B1 (no) 2005-06-20
JP3638599B2 (ja) 2005-04-13
ES2211870T3 (es) 2004-07-16
FI951633A0 (fi) 1995-04-05
EP0665890A1 (de) 1995-08-09
FI114482B (fi) 2004-10-29
FI924494A0 (fi) 1992-10-06
ATE254663T1 (de) 2003-12-15
JPH08501695A (ja) 1996-02-27
WO1994008024A1 (en) 1994-04-14
FI951633A (fi) 1995-04-05
CA2146240C (en) 2007-05-01
DK0665890T3 (da) 2004-03-08
NZ256425A (en) 1996-08-27
EE03074B1 (et) 1998-02-16
AU678402B2 (en) 1997-05-29
AU5112793A (en) 1994-04-26
CA2146240A1 (en) 1994-04-14
NO951315D0 (no) 1995-04-05

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Legal Events

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