DE602008002593D1 - Verfahren zur herstellung eines rekombinanten polyklonalen proteins - Google Patents

Verfahren zur herstellung eines rekombinanten polyklonalen proteins

Info

Publication number
DE602008002593D1
DE602008002593D1 DE602008002593T DE602008002593T DE602008002593D1 DE 602008002593 D1 DE602008002593 D1 DE 602008002593D1 DE 602008002593 T DE602008002593 T DE 602008002593T DE 602008002593 T DE602008002593 T DE 602008002593T DE 602008002593 D1 DE602008002593 D1 DE 602008002593D1
Authority
DE
Germany
Prior art keywords
collection
cells
recombinant polyclonal
polyclonal protein
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602008002593T
Other languages
English (en)
Inventor
Lars Soegaard Nielsen
Dietmar Weilguny
Anne Bondgaard Tolstrup
Finn Wiberg
Christian Mueller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Symphogen AS
Original Assignee
Symphogen AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symphogen AS filed Critical Symphogen AS
Publication of DE602008002593D1 publication Critical patent/DE602008002593D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
DE602008002593T 2007-05-25 2008-05-21 Verfahren zur herstellung eines rekombinanten polyklonalen proteins Active DE602008002593D1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200700764 2007-05-25
US92470807P 2007-05-29 2007-05-29
DKPA200701292 2007-09-07
US96000207P 2007-09-11 2007-09-11
PCT/DK2008/050116 WO2008145133A2 (en) 2007-05-25 2008-05-21 Method for manufacturing a recombinant polyclonal protein

Publications (1)

Publication Number Publication Date
DE602008002593D1 true DE602008002593D1 (de) 2010-10-28

Family

ID=39739965

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602008002593T Active DE602008002593D1 (de) 2007-05-25 2008-05-21 Verfahren zur herstellung eines rekombinanten polyklonalen proteins

Country Status (23)

Country Link
US (1) US7910332B2 (de)
EP (1) EP2152872B1 (de)
JP (2) JP5941616B2 (de)
KR (1) KR101555658B1 (de)
CN (1) CN101688204B (de)
AT (1) ATE481483T1 (de)
AU (1) AU2008255383B2 (de)
BR (1) BRPI0812091A2 (de)
CA (1) CA2683800C (de)
CY (1) CY1110939T1 (de)
DE (1) DE602008002593D1 (de)
DK (1) DK2152872T3 (de)
HK (1) HK1134519A1 (de)
HR (1) HRP20100640T1 (de)
IL (1) IL201395A (de)
MX (1) MX2009012526A (de)
NZ (1) NZ581316A (de)
PL (1) PL2152872T3 (de)
PT (1) PT2152872E (de)
SI (1) SI2152872T1 (de)
TW (1) TW200902548A (de)
WO (1) WO2008145133A2 (de)
ZA (1) ZA200908021B (de)

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* Cited by examiner, † Cited by third party
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PT1583830E (pt) 2003-01-07 2006-11-30 Symphogen As Método para produzir proteínas policlonais recombinantes
NZ578851A (en) * 2007-03-01 2011-09-30 Symphogen As Method for cloning cognate antibodies
JP5941616B2 (ja) * 2007-05-25 2016-06-29 シムフォゲン・アクティーゼルスカブＳｙｍｐｈｏｇｅｎ Ａ／Ｓ 組み換えポリクローナルタンパク質を製造する方法
WO2009030237A2 (en) * 2007-09-07 2009-03-12 Symphogen A/S Methods for recombinant manufacturing of anti-rsv antibodies
US20110003711A1 (en) * 2008-02-01 2011-01-06 Chromocell Corporation Cell lines expressing gaba receptor and methods using them
EP2098536A1 (de) 2008-03-05 2009-09-09 4-Antibody AG Isolation und Identifikation antigen- oder ligandenspezifischer Bindungsproteine
JP2011518790A (ja) * 2008-04-23 2011-06-30 シムフォゲン・アクティーゼルスカブ ポリクローナルタンパク質を製造する方法
CA2726289A1 (en) * 2008-05-28 2009-12-23 Bayer Healthcare Llc Methods and compositions for production of recombinant protein in hbx-expressing mammalian cells
BRPI0917352A2 (pt) * 2008-08-29 2017-08-22 Symphogen As Métodos para produção de uma biblioteca de pares cognatos que compreende sequências ligadas que codificam regiões variáveis e para gerar um vetor que codifica um anticorpo quimérico com regiões de seres humanos constantes e regiões variáveis não humanas, biblioteca de vetores, e método para produção de uma biblioteca de sequências de imunoglobulina derivadas de aves
WO2010089387A1 (en) 2009-02-09 2010-08-12 Morphosys Ag Production of oligoclonal mixtures of immunoglobulins in single cells
WO2012059858A1 (en) 2010-11-01 2012-05-10 Symphogen A/S Anti-her3 antibodies and compositions
EP2640743B1 (de) * 2010-11-16 2016-10-05 Excelimmune, Inc. Verfahren zur herstellung rekombinanter proteine
CN102558353A (zh) * 2011-12-09 2012-07-11 上海应用技术学院 一种大肠杆菌NrfA基因表达蛋白多克隆抗体及其制备方法
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
EP2844675B1 (de) 2012-05-02 2019-01-02 Symphogen A/S Humanisierte pan-her-antikörperzusammensetzungen
EP2953970A4 (de) 2013-02-08 2016-06-29 Misfolding Diagnostics Inc Transthyretinantikörper und verwendungen davon
IL250902B (en) 2014-09-16 2022-08-01 Symphogen As Anti-met antibodies and preparations
US20170044608A1 (en) * 2015-07-17 2017-02-16 Allele Biotechnology & Pharmaceuticals, Inc. Methods of selecting antibodies and antibody fragments
WO2017121896A1 (en) * 2016-01-14 2017-07-20 Anton Bauer Method of producing a production cell line
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
CA3185303A1 (en) 2017-04-05 2018-10-11 Symphogen A/S Combination therapies targeting pd-a, tim-3, and lag-3
SG11202012713RA (en) 2018-06-22 2021-01-28 Genmab As Method for producing a controlled mixture of two or more different antibodies
CN109988751A (zh) * 2019-01-15 2019-07-09 济宁学院 一种用于制备抗egfr全人源化单克隆抗体的细胞株构建方法
KR20220003055A (ko) * 2019-04-30 2022-01-07 기가젠, 인코포레이티드 재조합 폴리클로날 단백질 및 이의 사용 방법
KR20220069052A (ko) * 2019-09-27 2022-05-26 글락소스미스클라인 인털렉츄얼 프로퍼티 디벨로프먼트 리미티드 클론 세포주의 생산 안정성을 예측하는 방법

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016074A1 (en) 1990-04-19 1991-10-31 Medimmune, Inc. Administration of monoclonal antibodies against respiratory viruses
GB9022543D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Antibody production
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US20040038392A1 (en) * 2000-06-08 2004-02-26 Tatsurou Shibui Expression vector enabling screening of cells with high expression of recombinant protein, transformant with high expression of recombinant protein and utilization thereof
US6849259B2 (en) * 2000-06-16 2005-02-01 Symphogen A/S Polyclonal antibody composition for treating allergy
ES2442615T5 (es) * 2002-07-18 2023-03-16 Merus Nv Producción recombinante de mezclas de anticuerpos
AU2003283995A1 (en) 2002-09-30 2004-04-19 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
US20060275766A1 (en) * 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins
PT1583830E (pt) * 2003-01-07 2006-11-30 Symphogen As Método para produzir proteínas policlonais recombinantes
CN100383244C (zh) * 2003-01-07 2008-04-23 西福根有限公司 用于生产重组多克隆蛋白质的方法
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
KR101319848B1 (ko) * 2004-07-20 2013-10-18 심포젠 에이/에스 재조합 폴리클로날 단백질 또는 폴리클로날 세포주의구조적 특성화 방법
AU2005263331B8 (en) * 2004-07-20 2011-06-16 Symphogen A/S Anti-Rhesus D recombinant polyclonal antibody and methods of manufacture
US7850965B2 (en) 2005-12-05 2010-12-14 Symphogen A/S Anti-orthopoxvirus recombinant polyclonal antibody
WO2007101441A1 (en) 2006-03-06 2007-09-13 Symphogen A/S Recombinant polyclonal antibody for treatment of respiratory syncytial virus infections
JP5941616B2 (ja) * 2007-05-25 2016-06-29 シムフォゲン・アクティーゼルスカブＳｙｍｐｈｏｇｅｎ Ａ／Ｓ 組み換えポリクローナルタンパク質を製造する方法

Also Published As

Publication number Publication date
KR20100017923A (ko) 2010-02-16
AU2008255383A1 (en) 2008-12-04
PT2152872E (pt) 2010-11-17
BRPI0812091A2 (pt) 2014-10-14
HRP20100640T1 (hr) 2010-12-31
US7910332B2 (en) 2011-03-22
CN101688204B (zh) 2013-07-03
MX2009012526A (es) 2009-12-09
PL2152872T3 (pl) 2011-03-31
IL201395A0 (en) 2011-08-01
CN101688204A (zh) 2010-03-31
WO2008145133A2 (en) 2008-12-04
JP2011504721A (ja) 2011-02-17
WO2008145133A3 (en) 2009-02-05
US20090111142A1 (en) 2009-04-30
IL201395A (en) 2015-09-24
JP5941616B2 (ja) 2016-06-29
EP2152872B1 (de) 2010-09-15
ZA200908021B (en) 2010-11-24
EP2152872A2 (de) 2010-02-17
NZ581316A (en) 2012-02-24
DK2152872T3 (da) 2010-12-06
CY1110939T1 (el) 2015-06-10
CA2683800A1 (en) 2008-12-04
CA2683800C (en) 2015-03-17
TW200902548A (en) 2009-01-16
KR101555658B1 (ko) 2015-09-24
AU2008255383B2 (en) 2012-11-01
JP2015037411A (ja) 2015-02-26
HK1134519A1 (en) 2010-04-30
SI2152872T1 (sl) 2011-01-31
ATE481483T1 (de) 2010-10-15

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