CN219136773U

CN219136773U - Can avoid reagent pipe of pollution

Info

Publication number: CN219136773U
Application number: CN202320232647.1U
Authority: CN
Inventors: 王嘉铭; 史华; 王永生
Original assignee: Hebei Sheng Hua That Biologic Medical Science And Technology Ltd
Current assignee: Hebei Sheng Hua That Biologic Medical Science And Technology Ltd
Priority date: 2023-02-16
Filing date: 2023-02-16
Publication date: 2023-06-06
Anticipated expiration: 2033-02-16

Abstract

The utility model provides a reagent tube capable of avoiding pollution, comprising: a housing, a tube, and a charge assembly; the annular bottom surface of the shell is provided with a groove; the top of the pipe body is inserted into the groove of the shell and fixedly connected with the shell; the liquid medicaments are placed in the tube body, two medicaments are respectively filled in the reagent tube before the PCR experiment is carried out, the nucleic acid extraction and the nucleic acid amplification of the sample can be realized by distributing the reagents in the sealed reagent tube, and the reagent tube is not required to be opened again after the nucleic acid sample extraction step for filling the PCR reaction medicaments. After finishing the PCR test and treating the residue of the reagent tube, two medicaments can be respectively added again and then sealed, and the next experimental operation is carried out. The device has strong tightness, is simple and convenient to operate, can be repeatedly utilized, and avoids the waste of resources.

Description

Can avoid reagent pipe of pollution

Technical Field

The utility model relates to the technical field of PCR detection, in particular to a reagent tube capable of avoiding pollution.

Background

PCR is known collectively as polymerase chain reaction, also known as in vitro DNA amplification techniques. The PCR detection is a detection method of molecular biology, has high technical sensitivity, good repeatability and high yield, and is mostly applied to the biological fields of accounting detection and the like.

In the process of nucleic acid detection, firstly, a first reagent is required to be added to obtain a nucleic acid fragment in a sample, then, a second reagent is added to start PCR polymerase chain reaction to replicate and amplify the nucleic acid fragment to a certain concentration, and finally, a fluorescence experiment is utilized to detect whether the coronavirus nucleic acid fragment exists. And the sealing of the reaction cavity is ensured during the process, so that inaccurate detection data caused by water loss is prevented.

In the conventional nucleic acid detecting reagent tube, two kinds of reagents are placed in advance in the reagent tube, and a sample is injected after the reagent tube is sealed, so that the reaction can be performed in a sealed state. However, the structure of the reagent tube is changed in the process of filling the sample and the medicament, and the reagent tube must be discarded after one-time use, so that the resource waste is caused, and the cost of nucleic acid detection is increased.

Disclosure of Invention

The utility model aims to provide a reagent tube for avoiding pollution, which solves the problems in the prior art, ensures that the PCR reaction is carried out in a sealed state, and can realize repeated use of a single reagent tube.

In order to achieve the above object, the present utility model provides the following solutions: the utility model provides a reagent tube for avoiding pollution, which comprises: comprising the following steps: a housing, a tube, and a charge assembly; the annular bottom surface of the shell is provided with a groove; the top of the pipe body is inserted into the groove of the shell and fixedly connected with the shell; the tube body is internally provided with a liquid medicament.

A partition wall is formed in the middle of the bottom surface of the shell in an extending manner; the partition wall divides the inner cavity of the shell into a through groove and a spacing cavity; the through groove is communicated with the outside of the reagent tube; the top of the through groove extends inwards to form a clamping stagnation component; the blocking rubber is abutted to the inside of the clamping stagnation component; the top surface of the shell is provided with a circular channel; the bottom of the circular channel is communicated with the spacing cavity.

The charging assembly comprises a top cover and a charging tube; the top cover is detachably connected to the top of the charging tube through threads; the interior of the charging tube is hollow; the charging tube penetrates through the circular channel and enters the interval cavity; an inner groove is formed in the middle of the charging tube, and a rubber piston is embedded in the inner groove; the rubber piston is abutted with the inner wall of the circular channel; the bottom of the charge tube is fixedly connected with a charge bin; one side of the medicine bin is abutted with the side wall of the shell, and the other side of the medicine bin is abutted with the partition wall; an opening is formed in the lower part of the side wall of the medicine bin, which is close to one side of the partition wall; the medicine bin is internally provided with a spherical medicament.

The cap diameter is greater than the circular channel diameter.

The side wall of the medicine bin located at the bottom of the opening extends towards the notch of the partition wall and is abutted to the notch.

The bottom wall of the medicine bin is inclined downwards from one side close to the side wall of the shell to one side close to the partition wall, and the inclination angle is 15-20 degrees.

The side wall of the shell is communicated with the top wall of the interval cavity.

Sealing rubber is adhered to the top of the medicine bin; the sealing rubber is abutted against the partition wall and the inner wall of the shell.

The part of the clamping stagnation component, which is abutted against the top of the blocking rubber, is provided with a plurality of rectangular clamping teeth; the clamping block assembly is connected with the bottom of the blocking rubber in an abutting mode, and the clamping block assembly is matched with the through groove in shape.

A group of inward grooves are symmetrically formed in the top of the blocking rubber.

The diameter of the spherical medicament is smaller than that of the charging tube; the diameter of the spherical medicament is smaller than the caliber of the opening of the side wall of the medicament bin.

The through groove is semicircular in shape.

The utility model discloses the following technical effects:

1. according to the utility model, two medicaments are respectively distributed in the reagent tube, so that the nucleic acid extraction and the nucleic acid amplification reaction of the sample can be realized by distributing the medicaments in the sealed reagent tube, the reagent tube is not required to be opened to be filled with the PCR reaction medicament after the nucleic acid sample is extracted, the operation steps are reduced, and the risk of sample pollution is reduced.

2. The device ensures that the space cavity does not generate airflow flow with the tube body reaction area through the sealing rubber which is arranged as that the bottom surface of the medicine bin is abutted with the top of the opening of the partition wall, the side wall of the medicine bin is abutted with the inner wall of the shell and the partition wall and is positioned on the top surface of the medicine bin; the blocking rubber is abutted with the through groove clamping device, so that the outside does not generate airflow in the reaction area of the tube body. Ensures that the reaction is smoothly carried out in a closed environment.

3. After the single reaction is finished, the blocking rubber is taken out, distilled water is used for flushing the inside of the tube body, drying treatment is carried out for more than 1 hour, ultraviolet sterilization is carried out, and the interference of the residual nucleic acid fragments after the last reaction to the next experiment can be avoided. Then pouring liquid nucleic acid extraction reagent from the through groove, adding blocking rubber, taking down the top cover, putting into spherical PCR reagent, and then installing the top cover, namely, adding nucleic acid extraction reagent and nucleic acid amplification reagent again in a distributed manner, so that the next detection is facilitated. Repeating the above steps can achieve multiple reuse of a single reagent tube.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a front view of a cut-away of a body;

FIG. 2 is a top plan view;

FIG. 3 is a front view in partial section of the main body A;

1, a shell; 11. a through groove; 12. a circular channel; 13. a clamping stagnation component; 131. rectangular latch teeth; 132. a clamping table; 14. blocking rubber; 15. a partition wall; 16. a compartment; 17. a vent; 2. a tube body; 3. a charge assembly; 31. a top cover; 32. a charge tube; 33. a rubber piston; 34. a medicine bin; 35. sealing rubber; 51. a spherical medicament; 52. a liquid medicament.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In order that the above-recited objects, features and advantages of the present utility model will become more readily apparent, a more particular description of the utility model will be rendered by reference to the appended drawings and appended detailed description.

The utility model provides a reagent tube for avoiding pollution, which comprises: comprising the following steps: a housing 1, a tube 2 and a charging assembly 3; the annular bottom surface of the shell 1 is provided with a groove; the top of the pipe body 2 is inserted into a groove of the shell 1 and is fixedly connected with the shell 1; the tube body 2 has a liquid medicine 52 placed therein.

Preferably, the liquid agent 52 is an agent capable of extracting a sample nucleic acid fragment.

Preferably, the housing 1 and the charging assembly 3 are made of plastic, and the tube 2 is made of glass.

In one embodiment of the application, a partition wall 15 is formed in the middle of the bottom surface of the shell 1 in an extending manner; the inner cavity of the shell 1 is divided into a through groove 11 and a spacing cavity 16 by a partition wall 15; the through groove 11 is communicated with the outside of the reagent tube; the top of the through groove 11 extends inwards to form a clamping stagnation component 13; the blocking rubber 14 is abutted to the inside of the clamping stagnation assembly 13, and the blocking rubber 14 can isolate the inside of the pipe body 2 from the external air circulation, so that the air tightness of the inside of the device is ensured. The top surface of the shell 1 is provided with a circular channel 12; the bottom of the circular channel 12 communicates with the spacer chamber 16.

In one embodiment of the present application, the charge assembly 3 comprises a cap 31 and a charge tube 32; the top cap 31 can be dismantled through the screw thread and be connected in charge pipe 32 top, and the inside sealed state that is in of charge pipe 32 can be guaranteed to the top cap 31 when installing in charge pipe 32 top. The charge tube 32 is hollow inside, and the charge tube 32 penetrates through the circular channel 12 into the compartment 16; an inner groove is formed in the middle of the charging tube 32, and a rubber piston 33 is embedded in the inner groove; the rubber piston 33 abuts against the inner wall of the circular passage 12, so that the charge tube 32 can move up and down inside the circular passage 12. The bottom of the charge tube 32 is fixedly connected with a charge bin 34; one side of the medicine bin 34 is abutted with the side wall of the shell 1, and the other side is abutted with the partition wall 15, so that gas in the partition cavity 16 cannot flow into the tube body 2, and the air tightness in the reaction area of the tube body 2 is ensured.

Preferably, the cap 31 has a diameter greater than the diameter of said circular channel 12, limiting the lowest position of the charge assembly 3.

In one embodiment of the present application, the lower portion of the side wall of the medicine compartment 34 adjacent to the partition wall 15 is provided with an opening, and the inside of the medicine compartment 34 is filled with the spherical medicine 51. The charge assembly 3 is pushed down so that the opening is moved under the partition wall 15 through which the spherical medicament 51 can enter the interior of the reaction zone of the tube body 2.

Preferably, the spherical medicament 51 is a medicament for performing a polymerase chain reaction.

In a preferred embodiment of the present application, the bottom of the partition wall 15 is provided with a notch; the side wall of the medicine bin 34 positioned at the bottom of the opening extends towards the opening of the partition wall 15 and is abutted with the opening. The setting of opening has carried out spacingly to medicine storehouse 34 bottom surface, makes medicine charge assembly 3 have the biggest lifting height, and medicine storehouse 34 bottom surface and opening top butt have strengthened the inside gas tightness of device when the biggest lifting height.

In a preferred embodiment of the present application the bottom wall of the cartridge 34 is inclined downwards from the side close to the side wall of the housing 1 towards the side close to the partition wall 15 by an angle of 15-20, and the spherical medicament 51 can roll freely towards the opening under the influence of its own weight when the opening moves under the partition wall 15.

In a preferred embodiment of the present application, the side wall of the housing 1 and the top wall of the compartment 16 are connected with a vent 17, which ensures that the interior of the compartment 16 is in line with the outside air pressure and does not squeeze the cartridge 34.

In a preferred embodiment of the present application, the top of the cartridge 34 is bonded with a sealing rubber 35; the sealing rubber 35 is abutted against the partition wall 15 and the inner wall of the shell 1, so that the gas in the spacer cavity 16 does not enter the reaction area of the tube body 2, and the air tightness of the device is further enhanced.

In a preferred embodiment of the present application, the portion of the clamping stagnation component 13 abutting against the top of the blocking rubber 14 is a plurality of rectangular clamping teeth 131, and the rectangular clamping teeth 131 can prevent the blocking rubber 14 from being drawn out together when the sample injection needle tube is drawn out; the portion of the clamping stagnation component 13 abutting against the bottom of the blocking rubber 14 is a clamping table 132 matched with the through groove 11 in shape, and the clamping table 132 can prevent the blocking rubber 14 from being extruded into the tube body 2 when the sample injection needle tube is inserted.

In a preferred embodiment of the present application, a set of inward grooves are symmetrically formed in the top of the stopper rubber 14 to facilitate the extraction of the stopper rubber 14 by medical forceps or other instruments in the event of abutment of the stopper rubber 14 with the clamping assembly 13.

In one embodiment of the present application, the spherical medicament 51 has a diameter smaller than the diameter of the charge tube 32; the diameter of the spherical medicament 51 is smaller than the caliber of the side wall opening of the medicament bin 34.

In one embodiment of the present application, the through slot 11 is semi-circular in shape.

When the utility model is used for nucleic acid detection operation, firstly, liquid medicament 52 is poured into the tube body, and the blocking rubber 14 is embedded into the clamping stagnation component 13; the charging assembly 3 is lifted upwards to the highest position, the top cover 31 of the charging assembly 3 is opened, the spherical medicament 51 is placed in the charging tube 32, and the top cover 31 is twisted. The needle tube with the sample is injected into the tube body through the blocking rubber 14 to react with the liquid medicament 52 in the tube body, and the extraction of the nucleic acid fragments is completed. After the injection needle tube is pulled out, the blocking rubber 14 is automatically closed under the elastic action, so that a sealing space is formed. After the nucleic acid fragment extraction reaction is finished, pushing the medicine charging assembly 3 downwards to the bottommost part, enabling the spherical medicine 51 to slide into the tube body 2 through the opening of the side wall of the medicine bin 34, pushing the medicine charging assembly 3 to the highest part again, heating the tube body 2, enabling the spherical medicine 51 and the reagent in the tube body 2 to perform polymerase chain reaction, performing nucleic acid fragment amplification, namely, performing the whole guaranteed reaction in a closed environment, and finally performing a fluorescence experiment to detect whether coronavirus nucleic acid fragments exist.

After the detection of the single sample is completed, the stopper rubber 14 is pulled out based on the characteristic that the nucleic acid fragment is soluble in water and fails in a room temperature environment for 1 hour, and the reacted reagent is poured out from the through-tank 11. The inside of the tube body 2 is cleaned by distilled water, the drying treatment is carried out for 1 hour at normal temperature or high temperature, and then ultraviolet disinfection is carried out for 30 minutes, so that the condition that factors which interfere with the next experiment do not exist in the reagent tube can be ensured, and the charging steps are repeated, so that the next nucleic acid detection operation is carried out.

In the description of the present utility model, it should be understood that the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present utility model, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present utility model.

The above embodiments are only illustrative of the preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model, and various modifications and improvements made by those skilled in the art to the technical solutions of the present utility model should fall within the protection scope defined by the claims of the present utility model without departing from the design spirit of the present utility model.

Claims

1. A reagent tube capable of avoiding contamination, comprising: a shell (1), a tube body (2) and a charging assembly (3); the annular bottom surface of the shell (1) is provided with a groove; the top of the pipe body (2) is inserted into the groove of the shell (1) and is fixedly connected with the shell (1); a liquid medicament (52) is placed in the tube body (2);

a partition wall (15) is formed in the middle of the bottom surface of the shell (1) in an extending manner; the partition wall (15) divides the inner cavity of the shell (1) into two parts, namely a through groove (11) and a spacing cavity (16); the top of the through groove (11) is communicated with the outside; the top of the through groove (11) extends inwards to form a clamping stagnation component (13); a blocking rubber (14) is abutted to the inside of the clamping stagnation assembly (13); a circular channel (12) is formed in the top surface of the shell (1); the bottom of the circular channel (12) is communicated with the spacing cavity (16);

the charging assembly (3) comprises a charging bin (34) and a charging tube (32); the interior of the charging tube (32) is hollow; the charge tube (32) extends through the circular passage (12) into the compartment (16); the side wall of the charging tube (32) is abutted with the inner wall of the circular channel (12); the bottom of the medicine charging tube (32) is fixedly connected with a medicine bin (34); one side of the medicine bin (34) is abutted with the side wall of the shell (1), and the other side of the medicine bin is abutted with the partition wall (15); an opening is formed in the lower part of the side wall of the medicine bin (34) close to one side of the partition wall (15); the medicine bin (34) is internally provided with a spherical medicament (51).

2. A reagent tube capable of avoiding contamination according to claim 1, wherein: the top of the charging tube (32) is detachably connected with a top cover (31) through threads; the diameter of the top cover (31) is larger than that of the circular channel (12); an inner groove is formed in the middle of the charging tube (32), and a rubber piston (33) is embedded in the inner groove; the rubber piston (33) is abutted against the inner wall of the circular channel (12); the side wall of the shell (1) is communicated with the top wall of the spacing cavity (16) through a vent (17).

3. A reagent tube capable of avoiding contamination according to claim 1, wherein: a gap is formed at the bottom of the partition wall (15); the side wall of the medicine bin (34) positioned at the bottom of the opening extends towards the opening of the partition wall (15) and is abutted with the opening.

4. A reagent tube capable of avoiding contamination according to claim 1, wherein: the bottom wall of the medicine bin (34) is inclined downwards from one side close to the side wall of the shell (1) to one side close to the partition wall (15), and the inclination angle is 15-20 degrees.

5. A reagent tube capable of avoiding contamination according to claim 2, wherein: the top of the medicine bin (34) is adhered with sealing rubber (35); the sealing rubber (35) is in contact with the partition wall (15) and the inner wall of the housing (1).

6. A reagent tube capable of avoiding contamination according to claim 1, wherein: the part of the clamping stagnation component (13) which is abutted against the top of the blocking rubber (14) is provided with a plurality of rectangular clamping teeth (131); the part of the clamping stagnation component (13) abutting against the bottom of the blocking rubber (14) is a clamping table (132) with the shape matched with the through groove (11).

7. A reagent tube capable of avoiding contamination as recited in claim 6, wherein: a group of inward grooves are symmetrically formed in the top of the blocking rubber (14).

8. A reagent tube capable of avoiding contamination according to claim 1, wherein: the diameter of the spherical medicament (51) is smaller than the diameter of the charging tube (32); the diameter of the spherical medicament (51) is smaller than the caliber of the side wall opening of the medicament bin (34).

9. A reagent tube capable of avoiding contamination according to claim 1, wherein: the through groove (11) is semicircular in shape.