CN115436095A - Sampling and extracting assembly - Google Patents

Abstract

The invention discloses a sampling and extracting assembly, which comprises a sampling and extracting pipe body, a sealing cover and a drip nozzle cover, wherein the sampling and extracting pipe body is provided with a sampling and extracting pipe body; the sampling and extracting tube body is at least provided with a first cavity for washing a swab and a second cavity with a sealed top; at least one part of the pipe wall of the sampling and extracting pipe body is made of soft rubber, so that pressure is formed in the sampling and extracting pipe body when the sampling and extracting pipe body is extruded by external force; the drip nozzle cover is provided with an extrusion port communicated with the first cavity, so that the drip nozzle cover is sealed, the sampling extraction pipe body is extruded by external force, and the sampling extraction pipe body samples pass through the extrusion port to flow out. The sampling and extracting tube body is provided with the first cavity for washing the swab and the second cavity with the sealed top, and the test solution is pre-installed in the sampling and extracting tube body through the sealing surface, so that the on-site solution preparation is not needed, and the use convenience is improved; the dropper cover is arranged for dropwise adding the test solution after reaction, professional equipment such as a pipette is not needed, operation of untrained personnel and the old is facilitated, and the method is suitable for large-scale popularization and national nucleic acid detection.

Description

Sampling and extracting assembly

Technical Field

The invention relates to the technical field of medical instruments, in particular to a sampling and extracting assembly.

Background

In the fields of genetic engineering and protein engineering technology research, nucleic acid molecules are the main research objects, and the isolation and linearization of nucleic acids are the basic techniques of nucleic acid research. Nucleic acid is a basic unit representing the genetic characteristics of a living body, and nucleic acid detection is advanced biological detection on a molecular level, so that the method has the remarkable advantages of higher sensitivity, specificity, no window period and the like compared with the traditional morphological detection, cytology detection, immunology detection and the like. The nucleic acid detection comprises qualitative PCR, molecular hybridization, real-time fluorescence quantitative PCR and other technologies, and the first key of the nucleic acid detection technologies is to complete the extraction of nucleic acid of a biological sample, so that the effective and accurate extraction of a nucleic acid template becomes the premise of subsequent nucleic acid detection.

The general nucleic acid extraction process comprises three steps: lysis, rinsing and elution processes. The traditional extraction reagent has relatively low yield and complex steps, thus bringing certain influence on extraction work, and particularly being difficult to obtain qualified nucleic acid when the sample volume is rare. In addition, the traditional method has the disadvantages of multiple operation steps, large limitation, high cost, large sample requirement, easy cross contamination and inconvenience for customers to quickly extract and purify nucleic acid samples.

In addition, in the current real-time fluorescence PCR technology with the widest application of nucleic acid detection, the problems of complicated operation, high labor and cost, and the like generally exist, and complicated configuration work is required before use, so that errors and even failures of the test are easily caused. Different personnel operations also cause experimental errors, and especially when the operation is carried out outside a laboratory scene, the traditional nucleic acid sampling and extraction are more inconvenient, and the popularization of nucleic acid of the whole population is not facilitated. Therefore, in order to solve the problems of multiple operation steps, large limitation and inconvenient carrying, a sampling and extracting component is needed to be designed.

Disclosure of Invention

The invention aims to provide a sampling and extracting assembly, which solves the problems of multiple operation steps, large limitation and inconvenience in carrying in the existing nucleic acid detection.

The first technical scheme for realizing the purpose of the invention is as follows: a sampling and extracting assembly comprises a sampling and extracting pipe body, a sealing cover and a drip nozzle cover; the sampling and extracting tube body is at least provided with a first cavity for washing a swab and a second cavity with a sealed top;

at least one part of the pipe wall of the sampling and extracting pipe body is made of soft rubber, so that pressure is formed in the sampling and extracting pipe body when the soft rubber part is extruded by external force;

a first sealing part is arranged in a contact area of the drip nozzle cover and the sampling and extracting pipe body, so that the drip nozzle cover seals the sampling and extracting pipe body; the drip nozzle cover is provided with an extrusion opening communicated with the first cavity, so that the drip nozzle cover seals the sampling and extracting pipe body, and liquid in the sampling and extracting pipe body flows out through the extrusion opening when being extruded by external force;

the sampling extraction tube body and the contact area of the sealing cover are provided with a second sealing part, so that the sealing cover seals the sampling extraction tube body.

In some embodiments, the sealing member is preferably circular, the second sealing member has a larger diameter than the first sealing member, and the second sealing member is positioned lower on the sampling extraction tube body than the first sealing member; and when the sealing cover is used for sealing the sampling and extracting tube body, the drip nozzle cover connected with the sampling and extracting tube body through the second sealing part is sealed in the sealing cover.

Further, the inner wall of the sealing cover is provided with a sealing ring matched with the end face of the drip nozzle cover, so that the sealing cover and the drip nozzle cover are connected to each other, and when the sampling and extracting pipe body is sampled, liquid in the sampling and extracting pipe body is prevented from flowing out through the extrusion port.

In some embodiments, the sampling extraction tube is further provided with a third cavity for squeezing a swab; the third bottom surface of the third cavity is higher than the first bottom surface of the first cavity; a diversion port is arranged on the side wall of the third cavity facing the first cavity, so that the liquid in the third cavity flows into the first cavity through the diversion port.

In some embodiments, the top end of the second cavity is higher than the top end of the first cavity, forming a sealed platform; and a sealing surface is arranged on the sealing platform at the top end of the second cavity.

The sampling and extracting assembly further comprises a second piston guide rod; the cross section shape of the second piston guide rod is consistent with that of the second cavity; the second piston guide rod is provided with a guide groove in the direction towards the first cavity in a through manner, so that when the second piston guide rod is inserted into the second cavity, liquid in the second cavity rises to the top end face of the cavity along the guide groove and flows to the first cavity.

In some embodiments, the sampling extraction tube body is further provided with a fourth cavity and a fifth cavity; the top ends of the fourth cavity and the fifth cavity are flush with the top end of the second cavity to form a sealing platform; and sealing surfaces are arranged on the sealing platforms at the top ends of the fourth cavity and the fifth cavity.

In some embodiments, the second lumen is removably secured within the sampling extraction tube; the bottom of the second cavity is open; and a sealing surface is arranged outside the opening end part.

The fourth cavity and the fifth cavity are detachably fixed in the sampling and extracting tube body, slots for fixing the second cavity, the fourth cavity and the fifth cavity are arranged in the sampling and extracting tube body, and the bottoms of the fourth cavity and the fifth cavity are open; the opening end is provided with a sealing surface.

In some embodiments, the sample extraction assembly further comprises a fourth piston flow rod and a fifth piston flow rod; the cross section shapes of the fourth piston guide rod and the fifth piston guide rod are respectively consistent with the cross section shapes of the fourth cavity and the fifth cavity; the fourth piston guide rod and the fifth piston guide rod are respectively provided with a guide groove which faces the direction of the first cavity in a through manner, so that when the fourth piston guide rod and the fifth piston guide rod are respectively inserted into the fourth cavity and the fifth cavity, liquid in the fourth cavity and the fifth cavity rises to the top end face of the cavity along the guide grooves and flows to the first cavity.

Furthermore, when the fourth cavity and the fifth cavity are detachably fixed in the sampling and extracting tube body, a tube breaking head is arranged at one end, away from the extrusion part, of the piston diversion rod, so that the tube breaking head extends into the cavity to open the sealing surface at the bottom of the cavity.

In some embodiments, the sample extraction assembly further comprises a strong magnet; the strong magnet is positioned on the side wall of the bottom of the first cavity and used for adsorbing magnetic beads in the first cavity.

In some embodiments, the first cavity is not coaxial with the sampling extraction tube.

Further, the first cavity and the sampling and extracting tube share one side wall.

Furthermore, at least one side wall of the sampling and extracting tube body, which is shared by the first cavity and the sampling and extracting tube body, is made of soft rubber.

In some embodiments, at least one scraping wall for scraping a swab is also provided within the first cavity.

In some embodiments, the sampling and extracting assembly further comprises a buckle, wherein the buckle comprises a buckle plate arranged on one side of the sampling and extracting pipe body and a buckle arranged on the sealing cover and matched with the buckle plate, so that the buckle buckles the buckle plate when the sealing cover seals the sampling and extracting pipe body.

In some embodiments, a liquid outlet is arranged at the bottom of the first cavity, and a valve for controlling the opening and closing of the liquid outlet is arranged at the liquid outlet.

In some embodiments, the sample extraction assembly further comprises a first piston rod; the cross-sectional shape of the first piston rod is consistent with that of the first cavity, the liquid discharge port is opened, and when the first piston rod is inserted into the first cavity, liquid in the first cavity is discharged from the liquid discharge port.

In some embodiments, the sample extraction assembly further comprises a drain base; the liquid drainage base is provided with a strong magnetic base for placing a strong magnet and a waste liquid cavity for containing waste liquid discharged by the first cavity, so that the strong magnet fixed on the strong magnetic base is attached to the side wall of the first cavity when the sampling and extracting tube body is placed on the liquid drainage base; and the waste liquid cavity is communicated with a liquid outlet at the bottom of the first cavity.

Further, the waste liquid cavity pumps waste liquid in the first cavity into the waste liquid cavity through a liquid pump.

The second technical scheme of the invention is as follows: a method for using a sampling extraction component is specially used for the sampling extraction component, and comprises the following steps: a. inserting a swab containing a sample into a third cavity, extruding the sample on the swab, and flowing from a diversion port of the third cavity to the first cavity;

b. opening the second cavity, inserting the second piston guide rod, and extruding the liquid in the second cavity into the first cavity; after the reaction is finished, positioning the strong magnet to the side wall of the bottom of the first cavity, and adsorbing the magnetic beads in the first cavity; opening a liquid outlet at the bottom of the first cavity, inserting the first piston rod into the first cavity, and discharging liquid in the first cavity; closing a liquid discharge port at the bottom of the first cavity;

c. opening the fourth cavity, inserting the fourth piston guide rod, and extruding the liquid in the fourth cavity into the first cavity; after the reaction is finished, positioning the strong magnet to the side wall of the bottom of the first cavity, and adsorbing the magnetic beads in the first cavity; opening a liquid outlet at the bottom of the first cavity, inserting the first piston rod into the first cavity, and discharging liquid in the first cavity; closing a liquid discharge port at the bottom of the first cavity;

d. opening the fifth cavity, inserting the fifth piston guide rod, and extruding the liquid in the fifth cavity into the first cavity; after all reactions are finished, covering the drip nozzle cover, extruding the soft rubber part of the sampling and extracting pipe body, and extruding the liquid in the sampling and extracting pipe body from the extrusion port; and covering the sealing cover.

By adopting the technical scheme, the invention has the following beneficial effects:

(1) The sampling and extracting tube body is provided with the first cavity for washing the swab and the second cavity with the sealed top, and the test solution is pre-installed in the sampling and extracting tube body through the sealing surface, so that the on-site solution preparation is not needed, and the use convenience is improved; the dripping nozzle cover is arranged for dripping the test solution after reaction, professional equipment such as a pipette is not needed, the operation of untrained personnel and the operation of the old people are facilitated, and the method is suitable for large-scale popularization and national nucleic acid detection.

(2) The drip nozzle cover is sealed at the end part of the pipe orifice of the sampling and extracting pipe body through the sealing cover, and liquid is prevented from flowing out through the sealing ring, so that the integration is stronger, and the transportation is convenient.

(3) According to the invention, the sample adsorbed by the swab can be extruded out by inserting the swab into the third cavity, so that the release rate of the swab sample can be effectively improved, the concentration is improved, the detection sensitivity is increased, the operation is simple, and the popularization and the use are convenient.

(4) The sampling and extracting tube body is provided with a plurality of cavities for pre-filling test solutions, so that different test solutions can be pre-filled, and the solution is prevented from being prepared on site.

(5) The piston guide rod is used for guiding out the test solution in the cavity pre-filled with the test solution, so that the operation is simple and convenient, a professional pipettor is not required to operate, and the piston guide rod is suitable for popularization and promotion; be equipped with a plurality of different cavitys and be used for pre-installation different test solutions, application scope is wider.

(6) The cavity body pre-filled with the test solution is detachably fixed in the sampling extraction tube body, the bottom of the cavity body is provided with the opening, the test solution can be discharged from the bottom by puncturing the sealing surface at the opening, the use is more convenient and faster, and the operation difficulty is lower.

(7) The strong magnet is positioned through the liquid discharge base, the positioning is convenient, the operation is simple, the liquid discharge port is arranged at the bottom of the first cavity, the opening and closing of the liquid discharge port are controlled through the valve, liquid in the first cavity can be discharged without pouring, sampling and extracting the tube body, and the liquid waste is prevented from flowing back when the liquid waste is poured out, and the liquid waste is prevented from being poured out incompletely or flowing into other cavities to cause pollution.

Drawings

In order that the present disclosure may be more readily and clearly understood, reference is now made to the following detailed description of the present disclosure taken in conjunction with the accompanying drawings, in which

FIG. 1 is a schematic view of the present invention.

Fig. 2 is a schematic cross-sectional view of embodiment 1 of the present invention.

Fig. 3 is a schematic cross-sectional view of another state of embodiment 1 of the present invention.

Fig. 4 is a schematic view of the structure of embodiment 1 of the present invention, omitting the drip nozzle cover.

Fig. 5 is a schematic structural diagram of embodiment 1 of the present invention.

Fig. 6 is a schematic structural diagram of embodiment 2 of the present invention.

Fig. 7 is a schematic structural view of a sampling extrusion rod according to embodiment 2 of the present invention.

FIG. 8 is a schematic cross-sectional view of a sampling extrusion rod of example 2 of the present invention.

Fig. 9 is a schematic view of a sampling extraction tube omitting a second cavity in embodiment 3 of the present invention.

Fig. 10 is a schematic structural diagram of a second cavity, a fourth cavity, and a fifth cavity in embodiment 3 of the present invention.

Fig. 11 is a schematic structural diagram of a top view of a sampling extraction tube in embodiment 3 of the present invention.

Fig. 12 is a schematic view of the structure of a sampling extrusion rod in embodiment 3 of the invention.

Fig. 13 is a schematic cross-sectional view of embodiment 4 of the present invention.

The reference numbers in the figures are: the device comprises a sampling extraction tube body 1, a first cavity 11, a scraping wall 111, a second cavity 12, a third cavity 13, a flow guide opening 131, a fourth cavity 14, a fifth cavity 15 and a skirt 16; the sealing cover 2, the sealing ring 21; a drip nozzle cover 3, an extrusion port 31; a sampling extrusion rod 4, a second piston guide rod 41, a fifth piston guide rod 42, a guide groove 43 and a pipe breaking head 44; the device comprises a buckling component 5, a valve 6, a liquid discharge base 7, a strong magnet 71 and a waste liquid cavity 72.

Detailed Description

The general nucleic acid extraction process comprises three steps: lysis, rinsing and elution processes. The traditional extraction reagent has relatively low yield and complex steps, thus bringing certain influence on extraction work, and particularly being difficult to obtain qualified nucleic acid when the sample volume is rare. In addition, the traditional method has the disadvantages of multiple operation steps, large limitation, high cost, large sample requirement, easy cross contamination, difficult carrying, difficult operation for personnel who do not operate professionally, and incapability of realizing a family nucleic acid extraction and detection scene.

To above-mentioned problem, this application draws a sampling extraction element, can collect and gather, draw in an organic whole, need not the scene and join in marriage liquid, and basic thinking is: the utility model provides a sampling and extracting subassembly, draws body 1 including the sampling, sealed lid 2 and drip nozzle lid 3: the sampling and extracting tube body 1 comprises a first cavity 11 for washing a swab and a second cavity 12 for pre-loading a test solution, wherein the top of the second cavity 12 is higher than the top of the first cavity 11 to form a sealing platform; the sealing platform of the top end face of the second cavity 12 is provided with a sealing face.

Specifically, the sealing surface is preferably an aluminum foil or a plastic film.

Further, sealed lid 2 draws body 1 through first seal part and sampling to be connected, and first seal part draws the recess that body 1 is close to mouth of pipe department outer wall for setting up at the bulge loop of 2 inner walls of sealed lid and sampling, perhaps sets up and draws the bulge loop that body 1 is close to mouth of pipe department outer wall at the recess of 2 inner walls of sealed lid and sampling, adopts bulge loop and groove seal, simple structure, and is sealed effectual.

Further, the drip nozzle cover 3 is connected with the sampling extraction pipe body 1 through a second sealing part, the second sealing part extracts the groove of the pipe body 1 close to the outer wall of the pipe orifice for the convex ring of the inner wall of the drip nozzle cover 3 and the sampling extraction pipe body, or the groove of the inner wall of the drip nozzle cover 3 and the convex ring of the pipe body 1 close to the outer wall of the pipe orifice are extracted for the sampling extraction pipe body, the convex ring and the groove are adopted for sealing, the structure is simple, and the sealing effect is good.

The drip nozzle cover 3 is provided with an extrusion port 31, so that the drip nozzle cover 3 seals the sampling and extracting pipe body 1, and when the sampling and extracting pipe body 1 is extruded, the liquid in the sampling and extracting pipe body 1 flows out from the extrusion port 31.

(example 1)

Referring to fig. 1 to 4, the sampling and extracting assembly of the present embodiment includes a sampling and extracting tube 1, which is provided with a first cavity 11 for washing a swab and a second cavity 12 for pre-filling a test solution and sealing the top of the second cavity 12, wherein the top end of the second cavity 12 is higher than the top end of the first cavity 11 to form a sealing platform, which facilitates film sealing, the sealing platform on the top end surface of the second cavity 12 is provided with a sealing surface, and the sealing surface is preferably an aluminum foil or a plastic film.

At least one part of the pipe wall of the sampling and extracting pipe body 1 is made of soft rubber, so that pressure is formed in the sampling and extracting pipe body 1 when the soft rubber part is extruded by external force, and liquid in the sampling and extracting pipe body 1 is conveniently extruded.

Sealed lid 2 draws body 1 through first seal part and sampling to be connected, and first seal part draws the recess that body 1 is close to mouth of pipe department outer wall for setting up at the bulge loop of sealed 2 inner walls of lid and sampling, perhaps sets up and draws the bulge loop that body 1 is close to mouth of pipe department outer wall at the recess of sealed 2 inner walls of lid and sampling.

The drip nozzle cover 3 is connected with the sampling extraction pipe body 1 through a second sealing part, the second sealing part is a groove which is arranged on the outer wall of the drip nozzle cover 3 and is used for sampling the extraction pipe body 1 and is close to the outer wall of the pipe orifice, or a groove which is arranged on the inner wall of the drip nozzle cover 3 and is used for sampling the extraction pipe body 1 and is close to the outer wall of the pipe orifice.

Further, the drip nozzle cover 3 is hopper-shaped, and the 3 funnel-shaped bottoms of drip nozzle cover are equipped with and extrude mouth 31, make and draw body 1 at 3 sealed samplings of drip nozzle cover, and when the sampling draws body 1 and is extruded, the sampling draws the interior liquid of body 1 and flows out from extruding mouth 31. The precise control of the extrusion liquid amount is achieved by controlling the diameter of the extrusion opening 31 and the hydrophilicity and hydrophobicity of the material.

Further, as shown in fig. 2, the second sealing member and the first sealing member are both disposed on the outer wall of the circular sampling tube body, the diameter of the second sealing member is larger than that of the first sealing member, and the position of the second sealing member on the sampling tube body 1 is lower than that of the first sealing member. When the sealing cover 2 is used for sealing the sampling and extracting tube body 1, the drip nozzle cover 3 connected with the sampling and extracting tube body 1 through the second sealing part is sealed in the sealing cover 2.

Further, be equipped with in the sealed lid 2 with drip mouth lid 3 complex sealing ring 21, sealing ring 21 and the 2 terminal surface cooperations of sealed lid, when making sealed 2 sealed sampling extraction body 1 of sealed lid, 2 inner walls of sealed lid paste and tightly extrude mouth 31, sealing ring 21 compresses tightly at 3 tip of drip mouth lid to sealed mouth 31 of extruding.

Further, referring to fig. 3, the sealing cover 2 is provided with a portion engaged with the inner wall of the nozzle cover 3, and is provided with a protruding ring or a groove engaged with the second sealing member, so that the nozzle cover 3 can be fixed to the top end of the sealing cover 2.

Further, at least one scraping wall 111 for scraping the swab is disposed in the first cavity 11, so that when the swab is rotated in the first cavity, the sample on the swab is scraped by the scraping wall 111, and the sample is released more completely.

The sampling extraction assembly further comprises a buckling component 5, wherein the buckling component 5 comprises a buckling plate arranged on one side of the sampling extraction pipe body 1 and a buckle arranged on the sealing cover 2 and matched with the buckling plate, so that the buckling plate is buckled by the buckle when the sealing cover 2 is used for sampling the extraction pipe body 1 and sampling the extraction pipe body 1.

Referring to fig. 5, in some embodiments, the sampling extraction tube 1 is further provided with a third cavity 13 for squeezing the swab; the third bottom surface of the third cavity 13 is higher than the first bottom surface of the first cavity 11; the side wall of the third cavity 13 facing the first cavity 11 is provided with a diversion opening 131.

Specifically, the diversion opening 131 is a gap vertically formed in the side wall of the third cavity 13, and is simple in structure and convenient to mold.

Further, the inner diameter of the third cavity 13 is slightly smaller than the diameter of the sampling swab, so that when the sampling swab is inserted into the third cavity 13, the sampling swab is pressed by the inner wall of the third cavity 13, so that the sample adsorbed on the sampling swab flows from the diversion port 131 to the first cavity 11.

The present embodiment has at least the following advantages: the test solution is pre-installed in the sampling extraction tube body 1 through the sealing surface, so that the on-site solution preparation is not needed, and the use convenience is improved; the drip nozzle cover 3 is used for dripping the test solution after reaction, professional equipment such as a pipette is not needed, operation of untrained personnel and the old is facilitated, and the method is suitable for large-scale popularization; the drip nozzle cover 3 is sealed at the end part of the pipe orifice of the sampling and extracting pipe body 1 through the sealing cover 2, so that the integration is stronger, and the transportation is convenient; the third cavity 13 effectively improves the release rate of the swab sample, improves the concentration and increases the detection sensitivity; the swab is inserted into the third cavity 13, so that the sample adsorbed by the swab can be extruded, and the swab extruding device is simple to operate and convenient to popularize and use.

(example 2)

This embodiment is substantially the same as embodiment 1 except that: 6-8, the sample extraction assembly further comprises a second piston draft rod 41; the cross-sectional shape of the second piston guide rod 41 is consistent with that of the second cavity 12, so that the outer wall of the second piston guide rod 41 is attached to the inner wall of the second cavity 12 as much as possible; the second piston rod 41 is provided with a guiding groove 43 extending toward the first cavity 11, so that when the second piston rod 41 is inserted into the second cavity 12, the liquid in the second cavity 12 flows to the first cavity 11 along the guiding groove 43.

In some embodiments, the sampling extraction tube body 1 is further provided with a fourth cavity 14 and a fifth cavity 15; the top ends of the fourth cavity 14 and the fifth cavity 15 are flush with the top end of the second cavity 12, and form a sealed platform together with the top end of the second cavity 12.

Further, the sealing platform at the top end of the second cavity 12, the fourth cavity 14 and the fifth cavity 15 is provided with a sealing surface.

Further, the sampling extraction assembly further comprises a fourth piston draft rod and a fifth piston draft rod 42; the cross section shapes of the fourth piston guide rod and the fifth piston guide rod 42 are respectively consistent with the cross section shapes of the fourth cavity 14 and the fifth cavity 15; the outer wall of the fourth piston guide rod and the outer wall of the fifth piston guide rod 42 are respectively attached to the inner walls of the fourth cavity 14 and the fifth cavity 15 as much as possible, guide grooves 43 facing the direction of the first cavity 11 are formed in the fourth piston guide rod and the fifth piston guide rod 42 in a long mode, and when the fourth piston guide rod and the fifth piston guide rod 42 are respectively inserted into the fourth cavity 14 and the fifth cavity 15, liquid in the fourth cavity 14 and the fifth cavity 15 flows to the first cavity 11 along the guide grooves 43.

The application method of the embodiment comprises the following steps: a. inserting a swab containing a sample into the first cavity 11 for washing, and rotating the swab to dissolve the sample in a test solution in the first cavity;

b. inserting the washed swab into the third cavity 13, extruding a residual sample on the swab, flowing the residual sample from the flow guide port 131 of the third cavity 13 to the first cavity 11, positioning the strong magnet 71 to the side wall of the bottom of the first cavity 11 after the reaction is completed, and adsorbing magnetic beads in the first cavity 11; discharging the liquid in the first cavity 11;

c. opening the second cavity 12, inserting the second piston guide rod 41, and extruding the liquid in the second cavity 12 into the first cavity 11; after the reaction is finished, positioning the strong magnet 71 to the side wall of the bottom of the first cavity 11 to adsorb the magnetic beads in the first cavity 11; discharging the liquid in the first cavity 11;

d. opening the fourth cavity 14, inserting a fourth piston guide rod, and extruding the liquid in the fourth cavity 14 into the first cavity 11; after the reaction is finished, the strong magnet 71 is positioned on the side wall of the bottom of the first cavity 11 to adsorb magnetic beads in the first cavity 11; discharging the liquid in the first cavity 11;

e. opening the fifth cavity 15, inserting the fifth piston guide rod 42, and extruding the liquid in the fifth cavity 15 into the first cavity 11; after all reactions are finished, covering a drip nozzle cover 3, extruding the soft rubber part of the sampling and extracting pipe body 1, and extruding the liquid in the sampling and extracting pipe body 1 from an extrusion port 31; the sealing cover 2 is covered, and the sampling extraction tube body 1 and the drip nozzle cover 3 are sealed.

In some embodiments, the fourth chamber 14 conforms to the shape of the second chamber 12, the fourth piston rod conforms to the shape of the second piston rod 41, and the sampling swab, the second piston rod 41, and the fifth piston rod 42 are connected in sequence to form a sampling extrusion rod 4. After the use, the corresponding parts are broken, so that the use is more convenient, and the use of plastics is reduced.

This embodiment has at least the following advantages over embodiment 3: the device is provided with a plurality of cavities for pre-loading test solutions, so that different test solutions can be pre-loaded, the pre-loading requirements of various test solutions are met, and the solution is prevented from being prepared on site; the test solution in the cavity pre-filled with the test solution is led out through the piston guide rod, so that the operation is simple and convenient, the operation of a professional pipettor is not needed, and the piston guide rod is suitable for popularization and promotion; be equipped with a plurality of different cavitys and be used for preassembleing different test solutions, application scope is wider.

(example 3)

This embodiment is substantially the same as embodiment 1 except that: the second cavity 12 is detachably fixed in the sampling and extracting tube body 1; the bottom of the second cavity 12 is open; the opening end is externally provided with a sealing surface.

Referring to fig. 9, the fourth cavity 14 and the fifth cavity 15 are detachably fixed in the sampling extraction tube 1, a slot 16 for fixing the second cavity 12, the fourth cavity 14 and the fifth cavity 15 is arranged in the sampling extraction tube 1, and the bottom of the fourth cavity 14 and the bottom of the fifth cavity 15 are open; the opening end is provided with a sealing surface.

See fig. 10, specifically, the top end surfaces of the second cavity 12, the fourth cavity 14, and the fifth cavity 15 are provided with a brim engaged with the top end of the slot, so that after the second cavity 12, the fourth cavity 14, and the fifth cavity 15 are inserted into the slot, the brim abuts against the top end surface of the slot to prevent the brim from sliding into the sampling extraction tube 1.

Referring to fig. 11, in some embodiments, the second cavity 12, the fourth cavity 14, and the fifth cavity 15 are integrally formed, and a cap peak matched with the top end of the slot is disposed on the end surface of the top end, so that after the integrally formed second cavity 12, fourth cavity 14, and fifth cavity 15 are inserted into the slot, the cap peak abuts against the top end of the slot, and is prevented from sliding into the sampling extraction tube 1.

Referring to fig. 12, in some embodiments, the end of the sampling extrusion rod 4 is provided with a pipe breaking head 44, the pipe breaking head 44 is conical, and the cross section of the pipe breaking head is cross-shaped, so that a sealing surface can be conveniently punctured, and a cross-shaped cut can facilitate liquid drainage.

This example has at least the following advantages over example 3: the liquid in the second cavity 12, the fourth cavity 14 and the fifth cavity 15 can be led out more conveniently, and the difficulty is lower in split type processing.

(example 4)

This example is substantially the same as example 3, except that: referring to fig. 11, the sample extraction assembly further comprises a strong magnet 71; the strong magnet 71 is positioned on the sidewall of the bottom of the first chamber 11 for adsorbing the magnetic beads in the first chamber 11.

The first cavity 11 is not coaxial with the sampling extraction tube 1, and the first cavity 11 preferably shares a side wall with the sampling extraction tube 1.

Further, the strong magnet 71 is positioned by the liquid drainage base 7, the liquid drainage base 7 is matched with the bottom of the sampling and extracting tube body 1, and when the sampling and extracting tube body 1 is fixed on the liquid drainage base 7, the strong magnet 71 on the liquid drainage base 7 is tightly attached to the side wall of the first cavity 11.

In some embodiments, the first chamber 11 has a liquid outlet at the bottom, and the liquid outlet is provided with a valve 6 for controlling the opening and closing of the liquid outlet.

Specifically, the valve 6 is preferably a ball valve, so that the structure is stable, and the control is convenient.

Further, be equipped with the waste liquid chamber 72 that is used for holding first cavity 11 discharge waste liquid on the flowing back base 7, waste liquid chamber 72 is located first cavity 11 drain port below. Discharge more convenient, concentrate the waste liquid, make things convenient for follow-up recovery processing.

Further, the bottom of the sampling and extracting tube body 1 is also provided with a skirt 16 wrapped outside the first cavity 11, and the bottom end of the skirt 16 is lower than the bottom end of the first cavity 11, so that the sampling and extracting tube body can be conveniently inserted into and placed on a horizontal plane together with the liquid discharge base 7.

In some embodiments, the sampling and extracting assembly further comprises a first piston rod, and the cross-sectional shape of the first piston rod is consistent with that of the first cavity 11, so that the outer wall of the first piston rod is fitted with the inner wall of the first cavity 11 as much as possible; when the second piston guide rod 41 is inserted into the first cavity 11, the liquid in the first cavity 11 flows out through the liquid outlet with the bottom opened.

The way of discharging the liquid in the first cavity 11 is: positioning the strong magnet 71 to the side wall of the bottom of the first cavity 11, and adsorbing magnetic beads in the first cavity 11; and opening a liquid discharge port at the bottom of the first cavity 11, inserting the first piston rod into the first cavity 11, discharging liquid in the first cavity 11, and closing the liquid discharge port at the bottom of the first cavity 11.

This embodiment also has at least the following advantages over embodiment 3: the strong magnetism is positioned through the liquid discharge base 7, so that the positioning is convenient, and the operation is simple; the liquid outlet at the bottom of the first cavity 11 is controlled to be opened and closed through the valve 6, liquid in the first cavity 11 can be discharged without pouring the sampling extraction pipe body 1, and the phenomenon that the liquid is not completely poured or flows into other cavities to cause pollution due to backflow of the waste liquid when the waste liquid is poured is avoided; extrude liquid in the first cavity 11 through first piston rod, avoid leading to liquid can't flow because of liquid surface tension's influence, discharge more thoroughly, avoid liquid to remain.

The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (13)

1. A sampling extraction assembly, characterized by: comprises a sampling and extracting pipe body, a sealing cover and a drip nozzle cover; the sampling and extracting tube body is at least provided with a first cavity for washing swabs and a second cavity with a sealed top;

at least one part of the pipe wall of the sampling and extracting pipe body is made of soft rubber, so that pressure is formed in the sampling and extracting pipe body when the soft rubber part is extruded by external force;

a first sealing part is arranged in a contact area of the drip nozzle cover and the sampling and extracting pipe body, so that the drip nozzle cover seals the sampling and extracting pipe body; the drip nozzle cover is provided with an extrusion opening communicated with the first cavity, so that the drip nozzle cover seals the sampling and extracting pipe body, and liquid in the sampling and extracting pipe body flows out through the extrusion opening when being extruded by external force;

the sampling extraction tube body and the contact area of the sealing cover are provided with a second sealing part, so that the sealing cover seals the sampling extraction tube body.

2. A sample extraction assembly as claimed in claim 1, wherein: the inner wall of the sealing cover is provided with a sealing ring matched with the end face of the drip nozzle cover, so that the sealing cover and the drip nozzle cover are connected to each other in the sampling and extracting process of the pipe body, and liquid in the sampling and extracting process of the pipe body is prevented from flowing out of the extrusion port.

3. A sample extraction assembly as claimed in claim 1, wherein: the sampling and extracting tube body is also provided with a third cavity for squeezing the swab; the third bottom surface of the third cavity is higher than the first bottom surface of the first cavity; a diversion port is arranged on the side wall of the third cavity facing the first cavity, so that the liquid in the third cavity flows into the first cavity through the diversion port.

4. A sample extraction assembly as claimed in claim 1, wherein: the top end of the second cavity is higher than the top end of the first cavity to form a sealing platform; and a sealing surface is arranged on the sealing platform at the top end of the second cavity.

5. A sample extraction assembly as claimed in claim 4, wherein: the second cavity is detachably fixed in the sampling and extracting tube body; the bottom of the second cavity is open, and a sealing surface is arranged outside the open end.

6. A sample extraction assembly as claimed in either of claims 4 or 5, wherein: the sampling and extracting pipe body is also provided with a fourth cavity and a fifth cavity which are integrally formed with the sampling and extracting pipe body or the fourth cavity and the fifth cavity which are detachably fixed in the sampling and extracting pipe body; the top ends of the fourth cavity and the fifth cavity are flush with the top end of the second cavity to form a sealing platform; the sealing platform is provided with sealing surfaces for sealing the second cavity, the fourth cavity and the fifth cavity;

when the fourth cavity and the fifth cavity are detachably fixed in the sampling and extracting tube body, slots for fixing the second cavity, the fourth cavity and the fifth cavity are arranged in the sampling and extracting tube body, and the bottoms of the fourth cavity and the fifth cavity are open; the opening end is provided with a sealing surface.

7. A sample extraction assembly as claimed in claim 6, wherein: the sampling and extracting assembly further comprises a piston guide rod; the piston guide rod is provided with an extrusion part with the cross section shape consistent with that of the cavity; a flow guide groove facing to the direction of the first cavity is formed in the extrusion part of the piston flow guide rod in a through-length mode, so that when the piston flow guide rod is inserted into the cavity, liquid in the cavity rises to the end face of the top of the cavity along the flow guide groove and flows to the first cavity;

when the fourth cavity and the fifth cavity are detachably fixed in the sampling and extracting tube body, a tube breaking head is arranged at one end, far away from the extrusion part, of the piston guide rod, so that the tube breaking head extends into the cavity to open the sealing surface at the bottom of the cavity.

8. A sample extraction assembly as claimed in claim 1, wherein: the sampling and extracting assembly also comprises a strong magnet; the strong magnet is positioned on the side wall of the bottom of the first cavity and used for adsorbing magnetic beads in the first cavity.

9. A sample extraction assembly as claimed in claim 8, wherein: the first cavity and the sampling and extracting tube body are not on the same axis, and the first cavity and the sampling and extracting tube body share one side wall; the side wall of the sampling and extracting tube body, which is shared by at least the first cavity and the sampling and extracting tube body, is made of soft rubber.

10. A sample extraction assembly as claimed in claim 9, wherein: the bottom of the first cavity is provided with a liquid outlet, and the liquid outlet is provided with a valve for controlling the opening and closing of the liquid outlet.

11. A sample extraction assembly as claimed in claim 10, wherein: the sampling and extracting assembly further comprises a liquid discharge base; the liquid drainage base is provided with a strong magnetic base for placing a strong magnet and a waste liquid cavity for accommodating waste liquid discharged by the first cavity, so that the strong magnet fixed on the strong magnetic base is attached to the side wall of the first cavity when the sampling and extracting tube body is placed on the liquid drainage base; and the waste liquid cavity is communicated with a liquid outlet at the bottom of the first cavity.

12. A sample extraction assembly as claimed in claim 1, wherein: the sampling and extracting assembly further comprises a buckle, the buckle comprises a buckle arranged on the buckle arranged on one side of the sampling and extracting pipe body and a buckle arranged on the sealing cover and matched with the buckle, so that the sealing cover is sealed, and when the pipe body is extracted in the sampling, the buckle is buckled on the buckle.

13. A sample extraction assembly as claimed in claim 1, wherein: at least one scraping wall for scraping the swab is also provided within the first cavity.

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