CN213327562U

CN213327562U - Sample processing device

Info

Publication number: CN213327562U
Application number: CN202021934959.XU
Authority: CN
Inventors: 魏宏泉; 孙相鑫
Original assignee: Suzhou Yige Gene Technology Co ltd
Current assignee: Yihong (Shenzhen) gene Co.,Ltd.
Priority date: 2020-09-07
Filing date: 2020-09-07
Publication date: 2021-06-01
Anticipated expiration: 2030-09-07

Abstract

The utility model provides a sample processing device, which comprises a pushing mechanism, a sleeve and a sample processing tube, wherein one end of the sleeve is connected with the pushing mechanism, and the other end of the sleeve is detachably connected with one end of the sample processing tube; the other end of the sample processing pipe is provided with at least one liquid injection nozzle; and pushing out the liquid in the sample processing tube through the liquid injection nozzle by the pushing mechanism. The sample treatment tube realizes the extraction-free treatment and liquid feeding of the sample, and effectively improves the operation convenience.

Description

Sample processing device

Technical Field

The utility model relates to a technical field is examined to nucleic acid, concretely relates to sample processing apparatus.

Background

The gene detection technology is an important medical means for modern clinical diagnosis and disease prevention. The gene detection technology is increasingly regarded as the mainstream detection technology, and is widely applied to epidemiological screening at present. In addition, the results of gene testing provide important references for the prevention, analysis and treatment of genetic diseases.

The gene detection mainly comprises the following steps: sample collection and storage, sample submission, nucleic acid extraction, qPCR nucleic acid detection, result report and the like.

Wherein, the sample collection is to collect samples containing nucleic acid tissues or secretions such as throat swabs, saliva, blood and the like of a detected person. Then, the sample is stored in a specific sample collection tube and sent to a designated department to carry out nucleic acid extraction experiments in a specific laboratory in a unified manner. And after the nucleic acid extraction is finished, performing a fluorescence quantitative PCR (qPCR) experiment, analyzing nucleic acid components and abundance in the sample according to the experiment result, and finally generating a detection report.

The whole gene detection process is complicated in steps, requires multiple departments to cooperate with each other, and puts high requirements on experimental conditions and operators. The next day, the test report is generally available to the examinee.

SUMMERY OF THE UTILITY MODEL

The utility model provides a sample processing device.

One embodiment provides a sample processing device, which comprises a pushing mechanism, a sleeve and a sample processing tube, wherein one end of the sleeve is connected with the pushing mechanism, and the other end of the sleeve is detachably connected with one end of the sample processing tube; the other end of the sample processing pipe is provided with at least one liquid injection nozzle; and pushing out the liquid in the sample processing tube through the liquid injection nozzle by the pushing mechanism.

Optionally, the liquid pouring device further comprises a protective cap capable of plugging the liquid pouring nozzle.

Optionally, the protective cap is detachably connected to the bottom and/or the side wall of the sample processing tube.

Optionally, the protective cap has a cavity for accommodating the liquid injection nozzle, and when the protective cap covers the bottom of the sample processing tube, the inner bottom of the cavity is pressed against the liquid outlet of the liquid injection nozzle.

Optionally, the pushing mechanism includes a push rod and a piston disposed on the push rod, and the sleeve includes a hollow housing having a piston passage for movement of the piston.

Optionally, the number of the liquid injection nozzles is more than or equal to 2.

Optionally, the device further comprises an amplification plate, wherein a plurality of reaction tubes into which the liquid injection nozzles can be correspondingly inserted are arranged on the amplification plate.

Optionally, the bottom of the amplification plate is provided with support legs.

Optionally, the reaction tube further comprises a thin film which is used for closing the opening part of the reaction tube and can be broken by the liquid injection nozzle.

Optionally, the amplification plate comprises an upper amplification plate and a lower amplification plate, the upper amplification plate is provided with a plurality of liquid passing holes corresponding to the liquid injection nozzles, the lower amplification plate is provided with a plurality of liquid inlet holes corresponding to the liquid passing holes, and the lower amplification plate is provided with reaction tubes corresponding to the liquid inlet holes.

Optionally, the upper amplification plate is provided with a plurality of first grooves which can be matched with the bottoms of the sample processing tubes, and the liquid passing holes are arranged in the first grooves.

Optionally, a plurality of second grooves which can be matched with the outer bottoms of the first grooves are arranged on the lower amplification plate, and the liquid inlet hole is formed in the second grooves.

Optionally, the bottom of the first groove is provided with a liquid passing pipe which is communicated with the liquid passing hole and can be inserted into the reaction pipe.

Optionally, the liquid filling device further comprises a film which is used for closing the liquid passing hole and can be broken by the liquid filling nozzle.

Optionally, the upper amplification plate is a soft material.

Optionally, the soft material is selected from at least one of silica gel and plastic.

Optionally, a storage box for storing the sample processing tube and the amplification plate is further included.

Optionally, the storage box comprises an upper shell and a lower shell which are detachably connected, and the upper shell and the lower shell are covered to form a cavity which can accommodate the amplification plate inserted with the sample processing tube.

According to the sample processing device of the embodiment, the sample processing tube realizes the extraction-free processing and liquid adding of the sample, and the operation convenience is effectively improved.

Drawings

FIG. 1 is a schematic diagram of a sample processing device according to an embodiment;

FIG. 2 is a schematic view of a split structure of the sample processing device in one embodiment;

FIG. 3 is a schematic diagram of an embodiment of a protective cap;

FIG. 4 is a schematic view showing the structure of an amplification plate according to an embodiment;

FIG. 5 is a schematic view showing another structure of an amplification plate according to an embodiment;

FIG. 6 is a schematic view showing the structure of an upper amplification plate in one embodiment;

FIG. 7 is a schematic view showing another structure of an upper amplification plate in one embodiment;

FIG. 8 is a schematic view showing the structure of a lower amplification plate in one embodiment;

FIG. 9 is a schematic view showing another structure of a lower amplification plate in one embodiment;

FIG. 10 is a schematic view showing the structure of the sample processing tube inserted into the amplification plate according to one embodiment;

FIG. 11 is a flowchart showing the operation of gene detection in one embodiment.

The reference numbers illustrate:

1. a pushing mechanism; 101. a handle; 102. a push rod; 103. a piston; 2. a sleeve; 201. a housing; 202. a connecting cap; 203. A piston channel; 3. a sample processing tube; 301. a thread; 302. a liquid injection nozzle; 303. a liquid outlet; 4. a protective cap; 401. a concave cavity; 402. an inner bottom; 5. an upper housing; 6. an amplification plate; 61. an upper amplification plate; 611. a first groove; 612. a liquid passing hole; 613. a liquid passing pipe; 62. a lower amplification plate; 621. a second groove; 622. a liquid inlet hole; 623. a reaction tube; 63. supporting legs; 7. a lower housing; 8. an accommodating box.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

In view of the complicated operation of the existing gene detection process, the detection mechanism and the operators have higher requirements. In one embodiment, a high throughput genetic screening device is provided that addresses these issues. The device integrates nucleic acid extraction or treatment, qPCR detection and result report, reduces the requirements on gene detection mechanisms and operators, and makes the application of gene detection technology in small and medium-sized medical institutions and private clinics possible. In one embodiment, the device of the utility model has the advantages of high throughput, simple operation, convenience and rapidness, and has important application value for the diagnosis of hereditary diseases and the screening of epidemiology.

Example 1

As shown in fig. 1 and 2, in one embodiment, a sample processing device is provided, which includes a pushing mechanism 1, a sleeve 2, and a sample processing tube 3, wherein one end of the sleeve 2 is connected to the pushing mechanism 1, and the other end of the sleeve 2 is detachably connected to one end of the sample processing tube 3; the other end of the sample processing tube 3 is provided with at least one liquid injection nozzle 302; the liquid in the sample processing tube 3 is pushed out by the pushing mechanism 1 through the liquid injection nozzle 302, and the liquid can be added to the amplification plate 6.

Sleeve pipe 2 can be dismantled and be connected to sample processing pipe 3, is convenient for earlier to 3 liquid feeding backs of sample processing pipe, installs sleeve pipe 2 to sample processing pipe 3 again, and detachable construction is favorable to adding the sample fast in the sample processing pipe 3 and exempts from to carry liquid such as the mixed liquid of lysate.

In one embodiment, as shown in fig. 1 and 2, the liquid filling device further comprises a protective cap 4 capable of sealing the liquid filling nozzle 302, when the sample processing tube 3 is filled with the sample liquid, the protective cap 4 is sleeved on the bottom of the sample processing tube 3, and then the liquid in the sample processing tube 3 is shaken up, the protective cap 4 enables the liquid filling nozzle 302 at the bottom of the sample processing tube 3 to be effectively isolated and protected, and the liquid in the sample processing tube 3 is prevented from overflowing from the liquid filling nozzle 302 to cause pollution. The bottom of the sample processing tube 3 is the end of the sample processing tube 3 on which the liquid injection nozzle 302 is provided.

In an embodiment, the protective cap 4 is detachably connected to the bottom and/or the side wall of the sample processing tube 3. The protective cap 4 may in particular be connected to the bottom and/or the side wall of the sample processing tube 3 by means of a screw connection, an interference fit, a snap fit, etc.

In one embodiment, as shown in fig. 3, the protection cap 4 has a cavity 401 for accommodating the pouring nozzle 302, and when the protection cap 4 is closed to the bottom of the sample processing tube 3, the inner bottom 402 of the cavity 401 is pressed against the liquid outlet 303 of the pouring nozzle 302, so as to protect each pouring nozzle 302 and effectively prevent the liquid in the pouring nozzle 302 from overflowing due to shaking.

In an embodiment, as shown in fig. 2, 4, 8, and 9, the liquid injection nozzle 302 protrudes downward, and during liquid injection, the liquid injection nozzle 302 extends into the corresponding reaction tube 623 through the liquid inlet hole 622, which is beneficial for injecting liquid into the corresponding reaction tube 623, and thus liquid leakage or contamination is avoided.

In one embodiment, as shown in FIG. 2, the pushing mechanism 1 comprises a push rod 102 and a piston 103 disposed on the push rod 102, the sleeve 2 comprises a housing 201, the housing 201 has a piston channel 203 for moving the piston 103, and the piston 103 can move up and down in the piston channel 203 of the housing 201 to push the liquid in the sample processing tube 3 out of the liquid injection nozzle 302. When adding liquid, the worker removes the protective cap 4, holds the push rod 102 to apply force to the bottom of the sample processing tube 3, and the piston 103 moves towards the bottom of the sample processing tube 3, so as to add the sample liquid into the corresponding reaction tube 623 on the amplification plate 6.

In one embodiment, as shown in FIG. 2, the handle 101 is provided on the push rod 102, and when the worker holds the handle 101 and presses downward, the piston 103 will move downward, and due to the interference fit between the piston 103 and the inner wall of the housing 201, a downward gas pressure is generated, so that the liquid in the sample processing tube 3 flows out from the liquid injection nozzle 302. The handle 101 may be perpendicular to the push rod 102 for ease of manipulation.

In an embodiment, the number of the liquid injection nozzles 302 may be 1, 2, 3 or more, and the specific number is not limited and can be set as required. When a plurality of liquid injection nozzles 302 are provided, the liquid injection efficiency can be improved, and liquid can be injected into the plurality of reaction tubes 623 at a time.

In one embodiment, as shown in FIG. 2, the sleeve 2 is screwed to the sample processing tube 3, which allows for quick assembly and ease of use.

In one embodiment, as shown in fig. 2, the cannula 2 further comprises a connection cap 202 arranged at the bottom of the housing 201, the connection cap 202 having an inner diameter larger than the inner diameter of the housing 201, and the sample processing tube 3 having an outer diameter close to the inner diameter of the connection cap 202, such that the sample processing tube 3 can contain a sufficient amount of liquid. The outer wall of the sample processing tube 3 is provided with threads 301 and the inner wall of the connection cap 202 is provided with threads that can mate with the threads 301 so that the connection cap 202 can be screwed to the outer wall of the sample processing tube 3.

In an embodiment, the cross-section of the sleeve 2 may be circular, square or other regular, irregular shapes, and the shape of the sleeve 2 is not limited.

In one embodiment, as shown in FIG. 2, the priming nozzle 302 is located at the bottom of the sample processing tube 3 to facilitate priming of the amplification plate 6 below.

In one embodiment, as shown in FIGS. 4, 5, and 8, the device further comprises an amplification plate 6, and the amplification plate 6 is provided with a plurality of reaction tubes 623 corresponding to the liquid injection nozzle 302. Under the action of the pushing mechanism 1, the liquid injection nozzle 302 correspondingly injects the liquid into each reaction tube 623 for the subsequent amplification reaction.

In one embodiment, the reaction tube 623 further comprises a membrane for sealing the opening of the reaction tube 623 and allowing the liquid injection nozzle 302 to penetrate therethrough, and the membrane functions to seal the reaction tube 623 so that reagents such as buffer solution pre-filled in the reaction tube 623 before use do not overflow due to transfer, tilting, or turning over of the amplification plate.

In one embodiment, as shown in fig. 4, 5, 6, 7, and 8, the amplification plate 6 includes an upper amplification plate 61 and a lower amplification plate 62, the upper amplification plate 61 is provided with a plurality of liquid passing holes 612 corresponding to the liquid injection nozzles 302, the lower amplification plate 62 is provided with a plurality of liquid inlet holes 622 corresponding to the liquid passing holes 612, and the lower amplification plate 62 is provided with reaction tubes 623 corresponding to the liquid inlet holes 622. During liquid injection, the liquid injection nozzle 302 penetrates through the liquid through hole 612 and extends into the corresponding liquid inlet hole 622.

In one embodiment, a membrane is further included for closing the liquid through hole 612 and is penetrable by the liquid pouring nozzle 302. The membrane serves to block the through-hole 612 and thus the reaction tube 623, so that reagents such as a buffer solution pre-loaded in the reaction tube 623 before use do not overflow due to transfer, tilt, or inversion of the amplification plate. The material of the film can be silica gel, plastic and the like. The material of the film can be the existing soft material.

In one embodiment, the upper amplification plate 61 may be made of a soft material, such as silicon rubber, soft plastic, etc., which facilitates the rapid mounting of the upper amplification plate 61 to the lower amplification plate 62. The pushing mechanism 1, the sleeve 2, the sample processing tube 3, the protective cap 4, the lower amplification plate 62, the storage cassette 8, and the like may be made of PC, PVC, or the like.

In an embodiment, as shown in fig. 4, 5, and 8, the bottom of the amplification plate 6 is provided with support legs 63, when the amplification plate 6 is placed in the lower case 7, the support legs 63 support the amplification plate 6, so that the amplification plate 6 can be stably placed on a table or an instrument, and after the experiment is finished, the amplification plate can also be stably placed in the storage box 8. The support legs 63 can be located at four corners of the bottom of the amplification plate 6, and serve as stable supports.

In one embodiment, as shown in fig. 6, a plurality of first grooves 611 corresponding to the bottoms of the sample processing tubes 3 are formed on the upper amplification plate 61, the liquid passing holes 612 are disposed in the first grooves 611, the positions of the liquid passing holes 612 correspond to the liquid injection nozzle 302, the shapes of the first grooves 611 can be matched with the shapes of the bottoms of the sample processing tubes 3, and during liquid injection, the bottoms of the sample processing tubes 3 are matched with the first grooves 611, so that accurate alignment is facilitated, and the liquid injection efficiency is improved. After the liquid injection is finished, the sample processing tube 3 is kept inserted into the reaction tube 623 and does not need to be taken out, so that the liquid in the sample processing tube 3 is effectively prevented from overflowing from the liquid injection nozzle 302 to cause pollution.

In an embodiment, as shown in fig. 6 and 7, a liquid passing tube 613 connected to the liquid passing hole 612 and capable of being inserted into the reaction tube 623 is disposed at the bottom of the first groove 611, the liquid passing tube 613 is hollow, and the liquid injection nozzle 302 can pass through the liquid passing tube 613 and enter the reaction tube 623 to achieve liquid injection. The liquid passing tube 613 can be inserted into the reaction tube 623 to realize accurate alignment, and the liquid passing tube 613 plays a limiting role in the upper amplification plate 61, so that the upper amplification plate 61 can be conveniently installed on the lower amplification plate 62, and the displacement of the upper amplification plate 61 is avoided. The upper amplification plate 61 may be made of a soft material such as silica gel, and has a certain elasticity, so that the liquid passing tube 613 can be interference-fitted to the corresponding reaction tube 623.

In an embodiment, as shown in fig. 6, 7, 8, and 9, the bottom of the first groove 611 is downwardly convex, the lower amplification plate 62 is provided with a plurality of second grooves 621 corresponding to the outer bottom of the first groove 611, and the liquid inlet 622 is disposed in the second grooves 621, such that the outer sidewall of the first groove 611 can be correspondingly matched to the second grooves 621, and the upper amplification plate 61 can be rapidly mounted on the lower amplification plate 62.

In one embodiment, as shown in fig. 6 and 7, the bottom of the sample processing tube 3 is circular, and the first groove 611 is also circular, and the inner diameter thereof is slightly larger than the outer diameter of the bottom of the sample processing tube 3, so that the bottom of the sample processing tube 3 can be quickly matched with the first groove 611, thereby facilitating quick liquid injection, and facilitating the stable insertion of the sample processing tube 3 into the reaction tube 203, thereby preventing the sample processing tube 3 from tilting after a worker releases his/her hands.

In one embodiment, as shown in FIG. 2, the bottom of the sample processing tube 3 is provided with three injection nozzles 302, and each first groove 611 is provided with three liquid through holes 612 corresponding to the three injection nozzles 302, so that three liquid through holes 612 can be injected simultaneously at a time.

In one embodiment, as shown in FIG. 8, the reaction tubes 623 protrude downward and have a certain reagent capacity so as to contain a desired amount of reaction solution.

In one embodiment, the upper amplification plate 61 and the lower amplification plate 62 are detachably connected, and particularly can be connected by a snap-fit method or the like, so that the assembly is convenient.

In one embodiment, as shown in fig. 4, the storage box 8 is further provided for storing the sample processing tubes 3 and the amplification plates 6.

In one embodiment, as shown in fig. 4, 5 and 10, the storage cassette 8 includes an upper housing 5 and a lower housing 7 detachably connected to each other, and the upper housing 5 and the lower housing 7 cover each other to form a chamber for accommodating the amplification plate 6 with the sample processing tube 3 inserted therein. The receiver after the lid closes can be put into appointed collection device in, avoids polluting. The storage cassette 8 may have another structure, and the amplification plates 6 with the sample processing tubes 3 inserted therein may be stored, for example, the storage cassette 8 may have a structure with an opening on the side, and the amplification plates 6 with the sample processing tubes 3 inserted therein may be placed in the storage cassette 8 from the opening on the side.

In one embodiment, a nucleic acid-free extraction reaction is performed on the sample in the sample processing tube 3 to obtain a hands-free extract. Under manual manipulation, quantitative hands-free draw solution was added to the qPCR amplification plate through the sample processing tube 3. In one embodiment, the qPCR amplification plate has a total of 100 large wells, i.e., 100 first grooves 611, each well has 3 small wells, and can simultaneously perform gene detection on 3 key gene loci of 100 patients.

In one embodiment, a collected sample (e.g., saliva, throat swab, etc.) of a subject is placed in the sample processing tube 3, and a lysis solution is added to perform a lysis process. After the reaction was completed, the quantitative hands-free draw solution was added to the qPCR amplification plate. And transferring the qPCR amplification plate to a gas bath heating device for qPCR amplification, and performing data analysis through a fluorescence detection device connected with the device and matched fluorescence signal processing software to generate a result report. After that, the qPCR amplification plate inserted with the sample processing tube 3 is taken out and placed in the storage box 8, specifically, the qPCR amplification plate inserted with the sample processing tube 3 is placed in the lower case 7, then the upper case 5 is covered on the lower case 7, and the storage box 8 covered is placed in a designated recovery device.

In one embodiment, the sample processing device is suitable for genetic screening of large-scale samples, such as COVID-19 screening in a population, deafness gene screening in a population, and the like.

In an embodiment, the utility model discloses having integrated each flow of current gene detection, having realized the portably and automatic of gene detection, it is simple more convenient to make the high flux screening of gene detection, has reduced the requirement to medical institution experimental conditions and operating personnel, makes the gene detection move towards civilization to become possible.

In an embodiment, the utility model relates to a high flux qPCR amplifys board is equipped with 100 inspection holes, and every downthehole 3 reaction tubes 623 that establish can detect the key locus of 3 genes simultaneously, has improved the accuracy that detects, reduces false negative rate, especially has important meaning to the big batch sample screening of epidemiology.

In one embodiment, the sample processing tube 3 of the present invention can be pre-filled with reagents such as lysis solution, the sleeve 2 is assembled to the sample processing tube 3, the bottom of the sample processing tube 3 is covered with the protective cap 4 to be packaged into a product, after the purchaser receives the product, the sleeve 2 is removed, the throat swab, saliva, blood and other samples are placed in the lysis solution of the sample processing tube 3, the sleeve 2 is then installed, after shaking, the protective cap 4 is removed after the reaction in the sample processing tube 3 is finished, the liquid injection nozzle 302 pierces the film of the sealed reaction tube 623 and extends into the reaction tube 623, the liquid is added to the reaction tube 623 on the amplification plate 6 by pressing the handle 101 of the pushing mechanism 1, the sample solution is mixed with the reagents pre-filled in the reaction tube 623, the sample processing tube 3 is kept inserted into the amplification plate 6, the amplification plate 6 is placed in a heating instrument to perform the reaction, after the reaction is finished, the amplification plate 6 with the sample processing tube 3 inserted therein is taken out and put into the storage box 8, and thus, the pollution-free recovery can be realized.

In one embodiment, as shown in fig. 11, the specific operation flow of the sample processing device is as follows:

firstly, a sample of a person to be tested, such as a throat swab, saliva, blood and the like, is collected, the sample is added into the sample processing tube 3 shown in fig. 2, a certain amount of lysate is added into the sample processing tube 3 in advance, reaction can occur after the sample is added, the connecting cap 202 of the sleeve 2 is connected to the outer wall of the sample processing tube 3 in a threaded mode, and RNA hands-free taking processing is conducted. After the treatment, the liquid injection nozzle 302 at the lower end of the sample treatment tube 3 is aligned with the reaction tube 623 of the qPCR amplification plate, and the pushing mechanism 1 is pressed down to perform quantitative sample injection. After the sample is added, the qPCR amplification plate is placed in a gas bath qPCR instrument for amplification. And after amplification is finished, analyzing the acquired fluorescence data to generate a result report, and finishing the gene detection process.

It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.

Claims

1. A sample processing device is characterized by comprising a pushing mechanism, a sleeve and a sample processing tube, wherein one end of the sleeve is connected with the pushing mechanism, and the other end of the sleeve is detachably connected to one end of the sample processing tube; the other end of the sample processing pipe is provided with at least one liquid injection nozzle; and pushing out the liquid in the sample processing tube through the liquid injection nozzle by the pushing mechanism.

2. The sample processing device of claim 1, further comprising a protective cap that can seal off the pour spout; the protective cap is detachably connected to the bottom and/or the side wall of the sample processing tube.

3. The sample processing device as claimed in claim 2, wherein the protective cap has a cavity for receiving the pouring nozzle, and when the protective cap is closed to the bottom of the sample processing tube, the inner bottom of the cavity is pressed against the liquid outlet of the pouring nozzle.

4. The sample processing device of claim 1, wherein the pushing mechanism comprises a push rod and a piston disposed on the push rod, the sleeve comprising a hollow housing having a piston channel for movement of the piston;

the number of the liquid injection nozzles is more than or equal to 2.

5. The sample processing device according to claim 1, further comprising an amplification plate, wherein the amplification plate is provided with a plurality of reaction tubes into which the liquid injection nozzles are inserted.

6. The sample processing device of claim 5, wherein the bottom of the amplification plate is provided with support legs;

and the thin film is used for closing the opening part of the reaction tube and can be penetrated by the liquid injection nozzle.

7. The apparatus according to claim 5, wherein the amplification plate comprises an upper amplification plate and a lower amplification plate, the upper amplification plate is provided with a plurality of liquid passing holes corresponding to the liquid injecting nozzles, the lower amplification plate is provided with a plurality of liquid inlet holes corresponding to the liquid passing holes, and the lower amplification plate is provided with reaction tubes corresponding to the liquid inlet holes.

8. The sample processing device according to claim 7, wherein the upper amplification plate is provided with a plurality of first grooves which can be matched with the bottoms of the sample processing tubes, and the liquid passing holes are arranged in the first grooves;

the lower amplification plate is provided with a plurality of second grooves which can be matched with the outer bottoms of the first grooves, and the liquid inlet holes are arranged in the second grooves;

the bottom of the first groove is provided with a liquid passing pipe which is communicated with the liquid passing hole and can be inserted into the reaction pipe;

the film is used for sealing the liquid passing hole and can be broken by the liquid injection nozzle;

the upper amplification plate is made of soft materials;

the soft material is selected from at least one of silica gel and plastic.

9. The sample processing device according to any one of claims 6 to 8, further comprising a cartridge for receiving the sample processing tube, amplification plate.

10. The sample processing device of claim 9, wherein the cartridge comprises an upper housing and a lower housing that are detachably coupled, and the upper housing and the lower housing are closed to form a chamber for receiving the amplification plate into which the sample processing tube is inserted.