CN215856069U - One-stop reactor - Google Patents

Abstract

The utility model discloses a one-stop reactor, which comprises a reaction tube and a tube cover, wherein the tube cover is covered on the reaction tube, and a reaction chamber is formed between the reaction tube and the tube cover; the bottom of the reaction chamber is provided with a reaction cavity for storing liquid reagent, and a medicament tube for storing the reagent to be added and a reagent releasing mechanism for releasing the reagent to be added stored in the medicament tube into the reaction cavity are arranged above the reaction cavity; an anti-loosening structure for preventing the tube cover from loosening is arranged between the reaction tube and the tube cover. According to the one-stop reactor, the anti-loosening structure is arranged between the reaction tube and the tube cover, so that the tube cover can be prevented from loosening, and the sealing performance of the reaction chamber is improved; after the reaction in the liquid reagent reaches the set condition, reuse reagent release mechanism will deposit in the intraductal reagent of waiting to add of medicament and release and participate in the detection in the liquid reagent, whole process all can guarantee that the reaction chamber is in airtight state all the time, avoids the sample to pollute, improves and detects the precision to have simple structure, convenient operation safety's advantage.

Description

One-stop reactor

Technical Field

The utility model belongs to the technical field of medical instruments, and particularly relates to a one-stop reactor.

Background

PCR, the polymerase chain reaction, is a molecular biology technique for detecting specific DNA fragments by amplification. The detection method is simple and easy to implement, has extremely wide application, and can obtain an accurate result only by a small amount of samples. During PCR detection, a sample is firstly sampled, and is usually collected into a sampling tube by using a swab, and then is extracted from the sampling tube into a kit, and finally is placed into an instrument for reaction.

Chinese patent No. CN201520594082.7 discloses a novel fluorescent quantitative PCR kit, which integrates reagent solutions required for PCR reaction together, and each reagent is connected through a pipe, and the on-off of each pipe is realized by using a piston valve, so that the complicated PCR reaction is simplified. The novel fluorescent quantitative PCR kit has the advantages that as the plurality of piston valves are used, the size is large, the heating rate is seriously influenced, and the detection time is prolonged; and the reagent tube can not directly sample through the swab, so that sample pollution or loss can be caused in the operation process, and the detection accuracy is reduced.

Disclosure of Invention

In view of this, the present invention provides a one-stop reactor, which has the advantages of avoiding sample contamination and improving detection accuracy.

In order to achieve the purpose, the utility model provides the following technical scheme:

a one-stop reactor comprises a reaction tube and a tube cover which is covered on the reaction tube, wherein a reaction chamber is formed between the reaction tube and the tube cover; the bottom of the reaction chamber is provided with a reaction cavity for storing liquid reagents, and a medicament tube for storing reagents to be added and a reagent releasing mechanism for releasing the reagents to be added stored in the medicament tube into the reaction cavity are arranged above the reaction cavity;

and an anti-loosening structure for preventing the tube cover from loosening is arranged between the reaction tube and the tube cover.

Further, the upper end of the reaction tube is provided with a cylindrical section, and the cylindrical section is in threaded fit with the tube cover.

Furthermore, the anti-loosening structure adopts a ratchet structure arranged between the pipe cover and the cylindrical section.

Further, the ratchet structure comprises an inner ratchet arranged on the inner side wall of the pipe cover and an outer ratchet arranged on the outer wall of the cylindrical section and used for being meshed with the inner ratchet.

Furthermore, the inner ratchet and the outer ratchet are both provided with a tooth back surface and a backstop surface; when force for driving the pipe cover to be screwed is applied to the pipe cover, the back surfaces of the teeth of the inner ratchet teeth and the outer ratchet teeth are in contact fit, and the pipe cover can rotate relative to the cylindrical section; when a force driving the pipe cover to be loosened is applied to the pipe cover, the backstop surfaces of the inner ratchet teeth and the outer ratchet teeth are in contact fit and the pipe cover is kept static relative to the cylindrical section.

Further, the backstop surface is located in the radial direction of the corresponding tube cover or the cylindrical section.

Furthermore, a limiting ring extending outwards in the radial direction is arranged on the cylindrical section, and the limiting ring is used for limiting and matching with the pipe cover.

Further, the wall of the reaction cavity is made of transparent materials; or, a transparent detection window is arranged on the reaction cavity.

Further, a heating part in a flat shape is arranged at the bottom of the reaction chamber, and the reaction chamber is arranged in the heating part.

Further, the reaction cavity comprises a lower cavity and an upper cavity, and the distance between the two side walls of the lower cavity is smaller than the distance between the two side walls of the upper cavity.

Furthermore, the distance between the two side walls of the lower chamber is less than or equal to 1.5mm, and the sum of the volumes of the lower chamber and the upper chamber is greater than or equal to 110 ul.

Furthermore, heat conduction holes are respectively formed in two side walls of the reaction cavity, and heat conduction films cover the heat conduction holes.

Further, the reagent to be added stored in the medicament tube is a solid reagent, a liquid reagent or a powder reagent.

Further, the reagent to be added stored in the reagent tube is a solid reagent, and the solid reagent comprises at least one freeze-dried ball positioned below and a gravity ball positioned above all the freeze-dried balls and keeping the freeze-dried balls immersed in the liquid reagent after the solid reagent is released into the reaction cavity.

Further, the geometric dimension of the upper end opening of the reaction chamber is matched with the geometric dimension of the gravity ball and prevents the freeze-dried ball from floating out of the liquid reagent from one side of the gravity ball.

Further, the outer diameter of the gravity ball is larger than that of the freeze-dried ball.

Furthermore, a transition section is arranged between the cylindrical section and the reaction cavity, the geometric dimension of the inner wall of the transition section is gradually reduced along the direction from top to bottom, and the inner wall of the transition section and the reaction cavity are in smooth transition.

Further, the device also comprises a leakage-proof piece which is matched with the reaction tube and is used for preventing the liquid reagent stored in the reaction tube from leaking.

Further, the reactor also comprises a pipe sleeve used for covering the outside of the reaction pipe.

Further, a circumferential limiting structure used for preventing the pipe sleeve from rotating circumferentially relative to the cylindrical section is arranged between the pipe sleeve and the cylindrical section.

Furthermore, the circumferential limiting structure comprises a slot and a gear shaping which are matched with each other; the slots are annularly and uniformly distributed on the pipe sleeve, and the gear shaping is annularly and uniformly distributed on the cylindrical section; or the slots are annularly and uniformly distributed on the cylindrical section, and the gear shaping is annularly and uniformly distributed on the pipe sleeve.

Further, the gear shaping and the slots are arranged in a one-to-one correspondence mode, or the number of the slots is an integral multiple of the number of the gear shaping.

Furthermore, a limiting ring extending outwards in the radial direction is arranged on the cylindrical section, and the gear shaping is arranged on the bottom surface of the limiting ring.

Furthermore, the slot sets up on the upper end outer wall of pipe box, just the gear shaping with be equipped with between the outer wall of cylinder section and give way the clearance of giving way of pipe box.

Furthermore, a clamping structure used for clamping the heating part is arranged in the pipe sleeve.

Further, the clamping structure comprises an inverted buckle arranged in the pipe sleeve, and the heating part is provided with a buckle groove matched with the inverted buckle.

Further, the reaction chamber is internally provided with a slide rail positioned above the reaction chamber, and the medicament tube is arranged on the slide rail in a sliding fit manner.

Further, the slide rail is of a tubular structure with two open ends.

Further, a sealing structure is arranged between the medicament tube and the sliding rail.

Further, the sealing structure comprises a sealing plug which is arranged on the medicament tube and is in sealing fit with the slide rail, or the sealing structure comprises at least one first sealing ring which is arranged between the medicament tube and the slide rail.

Further, the lower extreme opening of slide rail coats and is stamped the seal membrane, perhaps be equipped with in the reaction chamber and be located the diaphragm between reaction chamber and the medicament pipe.

Furthermore, an isolation film covers the opening at the upper end of the sliding rail.

Further, the upper end of the medicament tube is closed, the lower end of the medicament tube is opened, and a positioning blind hole matched with the force application push rod is formed in the top surface of the medicament tube.

Further, the reagent release mechanism comprises a reagent release sleeve sleeved outside the reagent tube, a reagent release structure is arranged on the reagent release sleeve, and the reagent release structure is used for supporting the reagent to be added in a free state, preventing the reagent to be added from falling into the reaction cavity and releasing the reagent to be added under the action of downward force, so that the reagent to be added falls into the reaction cavity.

Further, the reagent release structure comprises at least two release holes which are annularly and uniformly distributed on the reagent release sleeve, release claws are arranged in the release holes, the bottoms of the release claws are bent inwards to form bent sections, and all the bent sections form a bottom support structure for supporting the lower end face of the reagent tube; when the reagent to be added adopts a solid reagent, the bottom support structure supports the solid reagent; when the reagent to be added adopts a liquid reagent or a powder reagent, the lower end face of the medicament tube is provided with an anti-falling film, and the bottom support structure is provided with a puncturing structure for puncturing the anti-falling film.

Furthermore, the root of the release claw is provided with a bending guide groove, or the release claw is made of elastic materials.

Furthermore, the side wall of the medicament tube is provided with an avoiding hole for the release claw to extend into.

Further, when the release claw is fully opened, the distance between the lower end part of the medicament tube and the lower end part of the reagent release sleeve is less than or equal to 3 mm.

Further, when the release pawl is fully opened, the lower end of the drug tube is aligned with the lower end of the reagent release sleeve.

Further, the slide rail is of a tubular structure with two open ends; a sealing film covers an opening at the lower end of the sliding rail, or a diaphragm positioned between the reaction cavity and the medicament tube is arranged in the reaction chamber; the bottom of the medicament release sleeve is provided with a dentate structure for puncturing the sealing membrane or the septum.

Furthermore, the dentate structure comprises puncture tips which are uniformly distributed in an annular manner, and a cutting edge is arranged between every two adjacent tips.

Further, the inner wall of slide rail is including being used for with medicament pipe complex cooperation section with be located the cooperation section below dodge the section, the internal diameter of cooperation section is less than dodge the section, the seal membrane is installed at the tip of dodging the section.

Furthermore, a slide rail positioned above the reaction cavity is arranged in the reaction chamber, and the medicament tube is arranged on the slide rail in a sliding fit manner; the medicament release sleeve and the medicament tube synchronously move downwards, and the lower end face of the medicament release sleeve is in limit fit with the inner wall of the reaction chamber above the reaction chamber.

Further, the medicament tube is positioned right above the reaction cavity.

Furthermore, the two side walls of the reaction cavity are symmetrically arranged, and the axes of the medicament tubes are positioned on the symmetrical surfaces of the two side walls of the reaction cavity.

Further, the lower port department of medicament release cover be equipped with be used for with spacing complex limit structure of medicament pipe.

The utility model has the beneficial effects that:

according to the one-stop reactor, the anti-loosening structure is arranged between the reaction tube and the tube cover, so that the tube cover can be prevented from loosening, and the sealing performance of the reaction chamber is improved; through set up the reaction chamber in the reaction chamber and be used for depositing liquid reagents such as swab sample, after the reaction in the liquid reagent reaches the settlement condition, recycle reagent release mechanism will leave in waiting that the medicament is intraductal to add reagent and release and participate in the detection in the liquid reagent, whole process homoenergetic can guarantee that the reaction chamber is in airtight state all the time, avoids the sample to pollute, improves and detects the precision to have simple structure, convenient operation safety's advantage.

The anti-loosening structure is set to be the ratchet structure, the inner ratchet arranged on the inner side wall of the pipe cover and the outer ratchet arranged on the cylindrical section are meshed with each other, and particularly, when force for driving the pipe cover to be screwed is applied to the pipe cover, the back sides of the teeth of the inner ratchet and the outer ratchet are in contact fit, the pipe cover can rotate relative to the cylindrical section, and therefore the pipe cover is screwed; meanwhile, the engagement between the inner ratchet and the outer ratchet can drive the pipe cover and the cylindrical section to slightly deform, and a user can judge whether the pipe cover is screwed in place from the aspects of force and sound, so that the screwing consistency between the pipe cover and the cylindrical section is ensured; when the effect of the power of its pine of drive is exerted to the tube cap, the surface contact cooperation of stopping of interior ratchet and outer ratchet makes the tube cap keep static for the cylinder section, when reverse application of force promptly, because the spacing effect between interior ratchet and the outer ratchet, the tube cap can't be unscrewed to can avoid the tube cap not hard up.

The reaction cavity is arranged into the lower cavity and the upper cavity, so that the liquid reagent capacity requirements of various reaction systems can be met; the space between the two side walls of the lower chamber is set to be smaller than the space between the two side walls of the upper chamber, namely the lower chamber has the characteristics of thin thickness and large heated area, so that the higher heating ratio is ensured, and the temperature of the reagent can be rapidly increased; the upper cavity is thicker than the lower cavity, so that the capacity of the reaction cavity can meet the requirements of different reaction systems.

Drawings

In order to make the object, technical scheme and beneficial effect of the utility model more clear, the utility model provides the following drawings for explanation:

FIG. 1 is a schematic structural view of an embodiment of a one-stage reactor according to the present invention;

FIG. 2 is an exploded view of a one-stage reactor according to the present embodiment;

FIG. 3 is a schematic structural view of a tube cover;

FIG. 4 is a schematic view of the structure of a reaction tube;

FIG. 5 is a schematic structural view of a reaction chamber;

FIG. 6 is a schematic view of the construction of the pipe sleeve;

FIG. 7 is a schematic view of a medicament release mechanism;

FIG. 8 is a schematic view of the structure of the cartridge;

FIG. 9 is a cross-sectional view of the one-station reactor of the present embodiment, particularly illustrating the structure of the medicament tube at the initial position;

FIG. 10 is a sectional view of the one-stop reactor of this embodiment, particularly illustrating the structure of the drug tube and the drug releasing sleeve moving downward;

fig. 11 is a cross-sectional view of the one-stop reactor of this embodiment, in which a specific drug release sleeve is in contact with and engaged with an inner wall of the reaction chamber and punctures the septum, and then releases the reagent to be added into the reaction chamber.

Description of reference numerals:

1-a reaction chamber; 2-a reaction chamber; 2 a-an upper chamber; 2 b-a lower chamber; 3-a medicament tube; 3 a-a guide groove; 3 b-avoiding holes; 4-heat conduction holes; 5-a heat conducting film; 6-a slide rail; 6 a-a mating segment; 6 b-an avoidance section; 7-a sealing plug; 8-a separator; 11-freeze-drying the balls; 12-a gravity ball; 13-a reaction tube; 13 a-a cylindrical section; 13 b-a transition section; 13 c-a heating section; 14-a tube cover; 15-a second sealing ring; 16-a leak prevention member; 17-a pipe sleeve; 19-a medicament release sleeve; 20-a release hole; 21-release of the jaws; 21 a-bending section; 22-bending a guide groove; 23-positioning blind holes; 24-a barrier film; 25-a toothed structure; 26-liquid reagent; 28-internal ratchet; 29-external ratchet; 30-slot; 31-gear shaping; 32-a stop collar; 33-abdication gap; 35-a piercing tip; 36-cutting edge.

Detailed Description

The present invention is further described with reference to the following drawings and specific examples so that those skilled in the art can better understand the present invention and can practice the present invention, but the examples are not intended to limit the present invention.

FIG. 1 is a schematic structural diagram of an embodiment of a one-stop reactor according to the present invention. The one-station reactor of this embodiment includes a reaction tube 13 and a tube cap 14 covering the reaction tube 13, and a reaction chamber 1 is formed between the reaction tube 13 and the tube cap 14. By providing the reaction chamber 1 as a separate body from the reaction tube 13 and the tube cover 14, it is possible to easily add a liquid reagent such as a swab sample into the reaction chamber 2. The bottom of the reaction chamber 1 is provided with a reaction chamber 2 for storing liquid reagent, and a reagent tube 3 for storing the reagent to be added and a reagent releasing mechanism for releasing the reagent to be added stored in the reagent tube 3 into the reaction chamber 2 are arranged above the reaction chamber 2. In the present embodiment, a loosening prevention structure for preventing the tube cap 14 from loosening is provided between the reaction tube 13 and the tube cap 14.

Specifically, the upper end of the reaction tube 13 of this embodiment is provided with a cylindrical section 13a, the cylindrical section 13a is in threaded fit with the tube cap 14, and a second sealing ring 15 is provided between the cylindrical section 13a and the tube cap 14 of this embodiment, so as to improve the sealing performance between the cylindrical section 13a and the tube cap 14. The anti-loosening structure of the present embodiment employs a ratchet structure provided between the tube cap 14 and the cylindrical section 13 a. Specifically, the ratchet structure includes an inner ratchet 28 provided on an inner side wall of the tube cover 14 and an outer ratchet 29 provided on an outer wall of the cylindrical section 13a for engagement with the inner ratchet 28. The inner ratchet 28 and the outer ratchet 29 of the embodiment are both provided with a tooth back surface and a backstop surface; when a force driving the tightening of the tube cap 14 is applied, the teeth of the inner ratchet 28 and the outer ratchet 29 are in back-contact engagement and the tube cap 14 is allowed to rotate relative to the cylindrical section 13a, thereby tightening the tube cap; meanwhile, the meshing between the inner ratchet and the outer ratchet can drive the pipe cover and the cylindrical section to slightly deform, and a user can judge whether the pipe cover is screwed in place or not from the aspects of force and sound, so that the screwing consistency between the pipe cover and the cylindrical section is ensured. When the tube cover 14 is acted by a force for driving the tube cover to loosen, the backstop surfaces of the inner ratchet 28 and the outer ratchet 29 are in contact fit and the tube cover 14 keeps static relative to the cylindrical section 13a, namely, when the force is applied reversely, the tube cover cannot be unscrewed due to the limiting function between the inner ratchet and the outer ratchet, so that the tube cover can be prevented from loosening, the tube cover 14 and the cylindrical section 13a are always kept in stable threaded fit, and the sealing performance of the second sealing ring 15 is ensured. Preferably, the retaining surface of the present embodiment is located in the radial direction of the corresponding tube cap 14 or the cylindrical section 13a, and when a force for driving the tube cap 14 to loosen is applied to the tube cap, the retaining surfaces of the inner ratchet 28 and the outer ratchet 29 are in contact fit with each other, so that the retaining surface is stressed integrally and has better stress performance. The cylindrical section 13a of this embodiment is provided with a radially outwardly extending stop ring 32, the stop ring 32 is adapted to engage with the tube cap 14 in a limiting manner, when the tube cap 14 engages with the ratchet structure of the cylindrical section 13a, a force and sound feedback is generated to the user, and at the same time, the tube cap 14 engages with the stop ring 32 in a limiting manner, so that the tube cap 14 cannot be unscrewed, indicating that the tube cap 14 has been screwed in place.

Further, the wall of the reaction chamber 2 is made of transparent material; or, a transparent detection window is arranged on the reaction cavity 2, so that the detection is convenient. The reaction chamber 2 of this embodiment is made of transparent material, so that the process of sample loading and rinsing can be conveniently observed.

Further, the bottom of the reaction chamber 1 is provided with a flat heating part 13c, and the reaction chamber 2 is arranged in the heating part, so that the liquid reagent is conveniently heated from the flat side walls on the two sides of the reaction chamber 2, and the heating efficiency is improved. Specifically, the reaction chamber 2 of the present embodiment includes a lower chamber 2a and an upper chamber 2b, and a distance between two sidewalls of the lower chamber 2a is smaller than a distance between two sidewalls of the upper chamber 2b, as shown in fig. 5. Preferably, the distance between the two side walls of the lower chamber 2a in this embodiment is less than or equal to 1.5mm, and the sum of the volumes of the lower chamber 2a and the upper chamber 2b is greater than or equal to 110ul, so that the rapid heating can be realized, and the volume requirements of various reaction systems can be met. The two side walls of the reaction chamber 2 of the embodiment are respectively provided with heat conduction holes 4, and the heat conduction holes 4 are covered with heat conduction films 5. The heat conduction holes 4 may be formed in both side walls of the lower chamber 2a, or the heat conduction holes 4 may be formed in both side walls of the lower chamber 2a and the upper chamber 2b, which will not be described in detail. Cover thermal-conductive membrane 5 on heat conduction hole 4, can improve heating efficiency and detection efficiency on the one hand, on the other hand: when the reaction cavity 2 is processed, a direct injection molding mode is generally adopted, but because the heating part is flat, the process difficulty of injection molding a thin-wall cavity is high, the processing precision cannot be guaranteed, and if the volume or the shape of the reaction cavity 2 is slightly deviated, the adding process and the reaction process of the reagent to be added can be influenced; after the heat conduction holes 4 are formed, the processing technology can be optimized to firstly inject a heating part with a through hole, the through hole is communicated with the inner wall of the transition section 13, then two heat conduction films 5 on the heating part seal the through hole, and a reaction cavity 2 is formed between the two heat conduction films 5; the processing mode is simple, and the processing precision of the reaction cavity is guaranteed. The reaction cavity is arranged into the lower cavity and the upper cavity, so that the liquid reagent capacity requirements of various reaction systems can be met; the space between the two side walls of the lower chamber is set to be smaller than the space between the two side walls of the upper chamber, namely the lower chamber has the characteristics of thin thickness and large heated area, so that the higher heating ratio is ensured, and the temperature of the reagent can be rapidly increased; the upper cavity is thicker than the lower cavity, so that the capacity of the reaction cavity can meet the requirements of different reaction systems. Of course, the reaction chamber 2 may have a tapered structure as shown in FIGS. 9 to 11, and may have a thin lower side to improve heating efficiency and a thick upper side to secure a capacity.

Further, the reaction chamber 1 is internally provided with an air hole 9 positioned above the reaction cavity 2 and used for balancing the internal pressure of the reaction chamber 1 so as to avoid the problem that the reagent tube is burst due to air pressure generated by the expansion of the internal gas of the reaction chamber 1 during heating. Be equipped with the filter core 10 that is used for filtering the aerosol in the bleeder vent 9 of this embodiment, prevent cross contamination, reduce personnel's operational risk. The ventilation hole 9 of the present embodiment is provided on the tube cover 14.

Further, the reagent to be added stored in the reagent tube 3 is a solid reagent, and the reagent to be added is described. In the embodiment, the reagent to be added stored in the reagent tube 3 is a solid reagent, and the reagent to be added comprises at least one freeze-dried ball 11 positioned below and a gravity ball 12 positioned above all the freeze-dried balls 11 and keeping the freeze-dried balls 11 immersed in the liquid reagent after the freeze-dried balls 11 are released into the reaction chamber 2, so that the freeze-dried balls 11 can be in sufficient contact with the liquid reagent to improve the dissolution rate of the freeze-dried balls 11. The freeze-dried pellets 11 of this embodiment are two in number. Preferably, the geometric dimension of the upper opening of the reaction chamber 2 matches with the geometric dimension of the gravity ball 12 and prevents the freeze-dried ball 11 from floating out of the liquid reagent from one side of the gravity ball 12, and the upper opening of the reaction chamber 2 of this embodiment is circular and has an inner diameter slightly larger or smaller than the outer diameter of the gravity ball 12. The geometric dimension of the reaction chamber 2 of the present embodiment is gradually reduced along the direction from top to bottom, and the gravity ball 12 is clamped at the upper opening of the reaction chamber 2. Specifically, the outer diameter of the gravity ball 12 is larger than that of the freeze-drying ball 11, so that the freeze-drying ball 11 can completely fall into the reaction chamber 2.

Further, be equipped with changeover portion 13b between cylinder section 13a and the reaction chamber 2, the inner wall geometric dimension of changeover portion 13b reduces along the direction from last to down gradually, and smooth transition between the inner wall of changeover portion 13b and the reaction chamber 2.

Further, the one-stop reactor of this embodiment further includes a leakage-preventing member 16, which is engaged with the reaction tube 13 and is used for preventing the liquid reagent stored in the reaction tube 11 from leaking, the leakage-preventing member 16 is used for plugging in the reaction tube 13 and is hermetically engaged with the reaction tube 13 to prevent the liquid reagent from leaking during transportation, and the leakage-preventing member 16 of this embodiment is a plug engaged with the reaction tube 11.

Further, the one-stop reactor of the embodiment further comprises a pipe sleeve 17 covering the reaction tube 13, an operator can hand the pipe sleeve to screw threads, the operation is convenient to hold, and the pipe sleeve 17 is made of transparent materials, so that the sample adding and rinsing process can be observed. A circumferential limit structure for preventing the pipe sleeve 17 from rotating circumferentially relative to the cylindrical section 13a is provided between the pipe sleeve 17 and the cylindrical section 13a of the present embodiment. Specifically, the circumferential limiting structure of the present embodiment includes a slot 30 and a gear tooth 31 which are matched with each other. The slots 30 and the gear shaping 31 are respectively arranged on the pipe sleeve 17 and the cylindrical section 13a, specifically, the slots 30 can be annularly and uniformly distributed on the pipe sleeve 17, and the gear shaping 31 is annularly and uniformly distributed on the cylindrical section 13 a; the slots 30 can also be annularly and uniformly distributed on the cylindrical section 13a, and the gear teeth 31 can be annularly and uniformly distributed on the pipe sleeve 17. The slots 30 of the embodiment are annularly and uniformly distributed on the pipe sleeve 17, and the gear teeth 31 are annularly and uniformly distributed on the cylindrical section 13 a. Of course, the number of the teeth 31 and the slots 30 may be equal, i.e., the teeth 31 and the slots 30 are arranged in a one-to-one correspondence. The number of the gear shaping 31 and the number of the slot 30 may not be equal, and the number of the slot 30 is an integral multiple of the number of the gear shaping 31. Specifically, the cylindrical section 13a of the present embodiment is provided with a radially outwardly extending limit ring 32, the gear shaping 31 is disposed on the bottom surface of the limit ring 32, and the limit ring 32 functions as the limit pipe sleeve 17 and the pipe cover 14. The slot 30 of the present embodiment is disposed on the outer wall of the upper end of the pipe sleeve 17, and a relief gap 33 of the relief pipe sleeve 17 is disposed between the gear 31 and the outer wall of the cylindrical section 13 a. Preferably, the jacket 17 of the present embodiment is provided with a clamping structure for clamping the heating unit, and serves to fix the bottom of the reaction tube 13. Specifically, the clamping structure comprises an inverted buckle arranged in the pipe sleeve 17, the heating part is provided with a buckle groove matched with the inverted buckle, when the gear shaping 31 is inserted into the slot 30, the inverted buckle and the buckle groove are buckled together, and the operation is convenient.

Further, a slide rail 6 positioned above the reaction cavity 2 is arranged in the reaction chamber 1, and the medicament tube 3 is arranged on the slide rail 6 in a sliding fit manner. The slide rail 6 of the present embodiment is a vertical slide rail. The slide rail 6 of this embodiment is the tubular structure of both ends opening, and the medicament pipe 3 sliding fit suit is in tubular slide rail 6.

Further, be equipped with seal structure between medicament pipe 3 and slide rail 6, seal structure is including setting up on medicament pipe 3 and with slide rail 6 sealing plug 7 of sealing up the complex, or seal structure is including setting up at least one first sealing washer between medicament pipe 3 and slide rail 6. The sealing structure of the present embodiment employs a sealing plug 7 provided on the drug tube 3 and sealingly engaged with the slide rail 6.

Furthermore, a sealing film covers the opening at the lower end of the slide rail 6, or a diaphragm 8 positioned between the reaction cavity 2 and the medicament tube 3 is arranged in the reaction chamber 1. Preferably, the opening at the upper end of the slide rail is covered with an isolation film 24, so as to further improve the sealing performance of the medicament tube 3. The sealing membrane, the isolating membrane and the diaphragm 8 are all easy-to-pierce membranes, and the diaphragm 8 positioned between the reaction cavity 2 and the medicament tube 3 is arranged in the reaction chamber 1, so that the technical purpose of isolating the to-be-added reagent and the liquid reagent is achieved.

Further, the lower extreme opening is sealed to medicament pipe 3 upper end, and is equipped with on the top surface of medicament pipe 3 and be used for with application of force push rod complex location blind hole 23, through location blind hole 23 with application of force push rod cooperation in order to drive medicament pipe 3 along slide rail 6 downstream.

Further, the reagent release mechanism of this embodiment includes that the suit is equipped with reagent release structure on the medicine release cover 19 outside medicine pipe 3 at medicine release cover 19, and reagent release structure is used for holding under free state and waits to add the reagent and prevent to wait to add the reagent and drop to reaction chamber 2 in, and be used for releasing under the effect of decurrent power and wait to add the reagent and make and wait to add the reagent and fall into reaction chamber 2. The reagent releasing structure of the embodiment comprises at least two releasing holes 20 annularly and uniformly distributed on the reagent releasing sleeve 19, releasing claws 21 are arranged in the releasing holes 20, the bottoms of the releasing claws 21 are bent inwards to form bent sections 21a, and all the bent sections 21a form a bottom support structure for supporting the lower end surface of the medicament tube 3. Specifically, the reagent to be added of this embodiment adopts solid reagent, and the collet structure still plays the effect that is used for holding solid reagent. Certainly, when the reagent to be added adopts a liquid reagent or a powder reagent, the lower end face of the medicament tube 3 can be provided with the anti-falling film, the bottom support structure is provided with a puncturing structure for puncturing the anti-falling film, and the puncturing structure can be arranged on the release claw 21, so that when the medicament tube 3 moves downwards to drive the release claw 21 to open outwards, the puncturing structure punctures the anti-falling film, and the liquid reagent and the powder reagent are released into the reaction cavity 2, and the technical purpose of medicament release can be realized. The medicament release sleeve 19 of the embodiment is provided with two release holes 20, and the bent sections 21a of the two release claws 21 form a bottom support structure for supporting the solid reagent, so that the solid reagent can be prevented from directly falling from the lower opening of the medicament tube 3.

Preferably, the base portion of the release pawl 21 of the present embodiment is provided with a bending guide groove 22, and the bending guide groove 22 can reduce the thickness of the base portion of the release pawl 21 to form a weak portion, so that the release pawl 21 can be bent in the bending guide groove 22 by a force. Of course, the releasing pawl 21 may also be made of an elastic material, and the elastic material is used to directly drive the releasing pawl 21 to generate elastic deformation.

Further, offer on the medicament pipe lateral wall and be used for releasing the hole of dodging 3b that claw 21 stretched into, so, release claw 21 is closed and stretch into medicament pipe 3 inside from dodging in the hole 3b when initial condition to constitute the collet structure that holds medicament pipe 3. Specifically, be equipped with the guide way 3a that is located on 3 lateral walls of medicament pipe between dodging hole 3b of this embodiment and the 3 bottom end face of medicament pipe, guide way 3a is used for the thickness of 3 walls of attenuate medicament pipe, when making medicament release cover 19 and medicament pipe 3 equipment, release claw 21 opens less angle and can enter into dodging in the hole 3b under the guide effect of guide way 3a, avoids appearing release claw 21 because of opening the too big problem that can't reset failure of angle. Specifically, when the reagent to be added moves downward, the medicine tube 3 can play a role in guiding, and the lower end of the medicine tube 3 can be located below the release claw 21 in such a manner that the avoiding hole 3b is used, that is, the length of the medicine tube 3 can be set to be sufficiently long. After the deformation of the releasing claw 21, the medicine to be added can be guided by the medicine tube 3 within a certain length in the downward moving process. Thus, when the reagent release sheath 19 is completely opened, the lower end of the drug tube 3 is aligned with the lower end of the reagent release sheath 19, and the guide distance is maximized. This is important for accurate entry of the reagent to be added into the reaction chamber 2. Especially, when the reagent to be added is the freeze-drying ball, if the freeze-drying ball is not pressed, the freeze-drying ball cannot completely enter the reaction cavity, adverse effects on the reaction are likely to be caused, the freeze-drying ball and the gravity ball in the embodiment are guided by the medicament tube 3 before entering the liquid level of the reaction cavity 2, the freeze-drying ball can be accurately pressed by the gravity ball after entering the liquid level of the reaction cavity 2, and the reaction can be fully performed.

Further, the slide rail 6 is a tubular structure with two open ends; a sealing film covers the lower end opening of the slide rail 6, or a diaphragm 8 positioned between the reaction cavity 2 and the medicament tube 3 is arranged in the reaction chamber 1; the bottom of the medicament release sleeve 19 of this embodiment is provided with a tooth-like structure 25 for piercing the sealing membrane or septum 8 for ease of handling. The tooth-like structure of this embodiment includes piercing points 35 which are annularly and uniformly arranged, and a cutting edge 36 is provided between two adjacent piercing points 35. The tooth mouth of the tooth-shaped structure 25 at the lower end of the medicament release sleeve 19 is 3/4 circles, so that eccentric force cannot be generated when the medicament release sleeve 19 is in contact with the reaction tube 13, and the freeze-drying ball is guaranteed to fall vertically. The number of the piercing tips 35 is 5 in this embodiment, and a cutting edge 36 is provided between two adjacent tips 35 to facilitate cutting of the sealing film or membrane 8.

Further, the inner wall of the slide rail 6 comprises a matching section 6a used for matching with the medicine tube 3 and an avoiding section 6b positioned below the matching section 6a, and the inner diameter of the matching section 6a is smaller than that of the avoiding section 6 b. When the lower end opening of the slide rail 6 is covered with the sealing film 8, the sealing film 8 is mounted on the end of the escape section 6 b. The sealing film 8 is generally welded or bonded to the end of the slide rail 6, and if the escape section 6b is not provided, the medicament release sleeve 19 may be blocked by the welding or bonding residue during the lowering process, or the residue may be attached to the tip of the tooth-like structure 25, which may affect the piercing of the sealing film.

Further, a slide rail 6 positioned above the reaction cavity 2 is arranged in the reaction chamber 1, and the medicament tube 3 is arranged on the slide rail 6 in a sliding fit manner; the medicament release sleeve 19 and the medicament tube 3 synchronously move downwards, and the lower end surface of the medicament release sleeve 19 is in limit fit with the inner wall of the reaction chamber 1 above the reaction chamber 2, so that the medicament release sleeve 19 is prevented from entering the reaction chamber 2. The reagent tube 3 of the present embodiment is located right above the reaction chamber 2, so that the reagent to be added can directly fall into the reaction chamber 2. Specifically, the two side walls of the reaction chamber 2 of the present embodiment are symmetrically disposed, and the axis of the drug tube 3 is located on the symmetric surfaces of the two side walls of the reaction chamber 2.

Further, a limiting structure used for limiting and matching with the medicament tube 3 is arranged at the lower end port of the medicament release sleeve 19. Specifically, the inner diameter of the lower opening of the drug release sleeve 19 of the present embodiment is smaller than the outer diameter of the drug tube 3 and larger than the outer diameters of the freeze-drying ball 11 and the gravity ball 12, so that the freeze-drying ball 11 and the gravity ball 12 can pass through while being in limit fit with the drug tube 3.

The use method of the one-stop reactor of the embodiment comprises the following steps: when in use, the tube cover 14 is unscrewed, the anti-leakage part 16 is taken out, the sample adding and washing operation is carried out, and then the tube cover 14 is covered back until the inner ratchet 28 and the outer ratchet 29 are mutually meshed; taking off the pipe sleeve 17, and placing the reagent pipe into an instrument for detection; a push rod in the instrument firstly pierces an isolating membrane 24 and then is in positioning fit with a positioning blind hole 23 to drive a medicament tube 3 and a medicament release sleeve 19 to move downwards, a tooth-shaped structure 25 arranged at the lower end of the medicament release sleeve 19 pierces a sealing membrane arranged at the bottom of a sliding rail 6 or a diaphragm 8 arranged between a reaction cavity 2 and the medicament tube 3 until the lower end face of the medicament release sleeve 19 is in spacing fit with the inner wall of a transition section 13b, at the moment, the push rod continuously drives the medicament tube 3 to move downwards, a release claw 21 is bent outwards and opened under the action of the force applied by the medicament tube 3 to enable the medicament tube 3 to pass through, the medicament tube 3 is in spacing fit with a spacing structure at the lower end port of the medicament release sleeve 19, a freeze-drying ball 11 and a gravity ball 12 respectively fall into the reaction cavity 2 from the lower end opening of the medicament tube 3, and the gravity ball 12 is clamped at the upper end opening of the reaction cavity 2 and is slightly higher than the liquid level of a liquid reagent, the lyophilized pellet 11 is immersed in a liquid reagent to accelerate the dissolution rate of the lyophilized pellet 11.

The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. The equivalent substitution or change made by the technical personnel in the technical field on the basis of the utility model is all within the protection scope of the utility model. The protection scope of the utility model is subject to the claims.

Claims (14)

1. A one-station reactor, comprising: the device comprises a reaction tube (13) and a tube cover (14) covered on the reaction tube (13), wherein a reaction chamber (1) is formed between the reaction tube (13) and the tube cover (14), and the bottom of the reaction chamber (1) is provided with a reaction cavity (2) for storing liquid reagents; and a loosening prevention structure for preventing the tube cover (14) from loosening is arranged between the reaction tube (13) and the tube cover (14).

2. The one-station reactor according to claim 1, wherein: the upper end of the reaction tube (13) is provided with a cylindrical section (13a), and the cylindrical section (13a) is in threaded fit with the tube cover (14).

3. The one-station reactor according to claim 2, wherein: the anti-loosening structure adopts a ratchet structure arranged between the pipe cover (14) and the cylindrical section (13 a);

the ratchet structure comprises an inner ratchet (28) arranged on the inner side wall of the tube cover (14) and an outer ratchet (29) arranged on the outer wall of the cylindrical section (13a) and used for being meshed with the inner ratchet (28).

4. The one-station reactor according to claim 3, wherein: the inner ratchet (28) and the outer ratchet (29) are respectively provided with a tooth back surface and a backstop surface; when force for driving the pipe cover (14) to be screwed is applied, the back surfaces of the teeth of the inner ratchet teeth (28) and the outer ratchet teeth (29) are in contact fit, and the pipe cover (14) can rotate relative to the cylindrical section (13 a); when a force driving the pipe cover (14) to be loosened is applied, the backstop surfaces of the inner ratchet teeth (28) and the outer ratchet teeth (29) are in contact fit and the pipe cover (14) is kept static relative to the cylindrical section (13 a).

5. The one-station reactor according to claim 4, wherein: the retaining surface is located in the radial direction of the corresponding tube cap (14) or the cylindrical section (13 a).

6. The one-station reactor according to any one of claims 2 to 5, wherein: the cylindrical section (13a) is provided with a limiting ring (32) extending outwards in the radial direction, and the limiting ring (32) is used for limiting and matching with the pipe cover (14).

7. The one-station reactor according to claim 2, wherein: the bottom of the reaction chamber (1) is provided with a flat heating part (13c), and the reaction cavity (2) is arranged in the heating part;

the reaction cavity (2) comprises a lower cavity (2a) and an upper cavity (2b), and the distance between two side walls of the lower cavity (2a) is smaller than the distance between two side walls of the upper cavity (2 b).

8. The one-station reactor according to claim 7, wherein: the distance between the two side walls of the lower chamber (2a) is less than or equal to 1.5mm, and the sum of the volumes of the lower chamber (2a) and the upper chamber (2b) is more than or equal to 110 ul.

9. The one-station reactor according to claim 7, wherein: the reaction tube also comprises a tube sleeve (17) used for covering the outside of the reaction tube (13);

a circumferential limiting structure for preventing the pipe sleeve (17) from rotating relative to the cylindrical section (13a) in the circumferential direction is arranged between the pipe sleeve (17) and the cylindrical section (13 a).

10. The one-station reactor according to claim 9, wherein: the circumferential limiting structure comprises a slot (30) and a gear shaping (31) which are matched with each other; the slots (30) are annularly and uniformly distributed on the pipe sleeve (17), and the gear shaping teeth (31) are annularly and uniformly distributed on the cylindrical section (13 a); or the inserting grooves (30) are annularly and uniformly distributed on the cylindrical section (13a), and the gear shaping teeth (31) are annularly and uniformly distributed on the pipe sleeve (17).

11. The one-station reactor according to claim 10, wherein: the gear shaping (31) and the slots (30) are arranged in a one-to-one correspondence mode, or the number of the slots (30) is the integral multiple of the number of the gear shaping (31).

12. The one-station reactor according to claim 10, wherein: the cylindrical section (13a) is provided with a limiting ring (32) extending outwards in the radial direction, and the gear shaping (31) is arranged on the bottom surface of the limiting ring (32).

13. The one-station reactor according to claim 12, wherein: the inserting grooves (30) are formed in the outer wall of the upper end of the pipe sleeve (17), and abdicating gaps (33) of the pipe sleeve (17) are formed between the gear shaping (31) and the outer wall of the cylindrical section (13 a).

14. The one-station reactor according to claim 9, wherein: a clamping structure for clamping the heating part is arranged in the pipe sleeve (17);

the clamping structure comprises an inverted buckle arranged in the pipe sleeve (17), and the heating part is provided with a buckle groove matched with the inverted buckle.

Images