CN208395201U - It is a kind of can pre-filled reagent S type microchannel formula nucleic acid amplification airtight reactor tube - Google Patents
It is a kind of can pre-filled reagent S type microchannel formula nucleic acid amplification airtight reactor tube Download PDFInfo
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- CN208395201U CN208395201U CN201820837403.5U CN201820837403U CN208395201U CN 208395201 U CN208395201 U CN 208395201U CN 201820837403 U CN201820837403 U CN 201820837403U CN 208395201 U CN208395201 U CN 208395201U
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Abstract
The utility model discloses it is a kind of can pre-filled reagent S type microchannel formula nucleic acid amplification airtight reactor tube, liquid storage tubular body is connected to the liquid storage chamber of lower end surface at least provided with two, at least containing two different kinds of polymerase liquid；Bottom cover upper surface is transversely provided at least two S type microchannels, and the second central through hole that the inner end of all S type microchannels is axially arranged with one is connected；Bottom cover is sealed in liquid storage tube body bottom surface, and the outboard end of every S type microchannel is connected with corresponding liquid storage chamber bottom end；The liquid in liquid storage chamber and reaction tank is separated using the air physical inside S type microchannel, thus user need not still further prepare any reagent, whole operation process is without unlatching sealing cover body, while realizing plurality of reagents prepackage, it also not will cause the stability of a variety of pre-filled reagents and validity period change, and can be transferred through Shooting Technique and realize large-scale production, significantly reduce application cost, it is especially suitable for multienzymatic reaction, there is apparent practical value.
Description
Technical Field
The utility model relates to a life science field Polymerase Chain Reaction (PCR for short) and derived amplifications consumptive material for experiments, what especially relate to is the airtight Reaction tube of S type microtube formula nucleic acid amplification that can pre-install reagent.
Background
PCR detection technology has been widely used in the detection of organisms, especially in the detection of pathogens, with the development of life science technology, various nucleic acid amplification technologies of different principles have been continuously derived; the emergence of the isothermal amplification technology enables the application of the PCR technology to be widely accepted, and meanwhile, the PCR technology has the development trend of on-site and miniaturization of molecular detection application and gradually expands from medical detection application to more basic application fields such as veterinary medicine, food safety and the like.
When PCR amplification detection is carried out, biological active materials such as polymerase and the like are often needed, a plurality of different biological enzymes such as reverse transcriptase and the like are often needed in the virus detection process, particularly, two or more biological enzymes are possibly used in the derived constant temperature amplification technology, the materials are stored at the low temperature of-20 ℃, and different biological enzymes need different storage buffer solution conditions.
However, the current practice is to store different enzyme solutions in different reagent tubes, and use them at the time of detection, which brings many problems to the detection application, for example: complicated operation, the risk of aerosol pollution in the liquid preparation process, the requirement of professional personnel and sites and the like are required; thereby limiting the application and popularity of the related art in the basement. If the complicated operation of preparing the solution can be avoided, the PCR and the derivative detection technology thereof are obviously beneficial.
However, due to the biological characteristics, after each component of the bioactive material, particularly the biological enzyme, is premixed in the same buffer solution, the biological activity is immediately reduced, so that the performances such as quality guarantee period, stability and the like are obviously reduced, and the influence on the detection result is great; some existing solutions achieve the purpose of premixing reagents by means of vacuum freeze drying; however, the detection cost is greatly increased due to the factors of severe process conditions, low finished product qualification rate, long process time and the like, and different enzymes still need to be respectively freeze-dried in the multi-enzyme reaction, so that the difficulty and uncertainty of the process are greatly increased.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a but S type microtube formula nucleic acid amplification airtight reaction tube of pre-installation reagent can simplify the mixing operation greatly and show reduction application cost when avoiding influencing the testing result, is particularly suitable for the multienzyme reaction.
The technical scheme of the utility model as follows: an S-shaped micro-pipeline type nucleic acid amplification closed reaction tube capable of being preloaded with reagents comprises a reaction tube body, a liquid storage tube body, a bottom cover and a sealing cover body; the bottom cover and the liquid storage tube body are both arranged in the reaction tube body, and the sealing cover body is plugged at the opening of the reaction tube body; wherein, a reaction tank for containing reaction liquid is arranged in the reaction tube body, and the outer wall of the sealing cover body is tightly matched with the inner wall of the reaction tube body; the upper part of the liquid storage tube body is provided with a liquid storage tank communicated with the upper end surface of the liquid storage tube body and used for injecting nucleic acid liquid to be detected before detection, the bottom of the liquid storage tank is communicated with the reaction tank through a first central through hole which is axially arranged, and the lower end surface of the sealing cover body is tightly contacted with the upper end surface of the liquid storage tube body after assembly; the liquid storage tube body is internally provided with at least two liquid storage cavities communicated with the lower end surface of the liquid storage tube body and used for containing at least two different types of polymerase liquid; the upper end face of the bottom cover is transversely provided with at least two S-shaped micro-pipelines, and the inner side ends of all the S-shaped micro-pipelines are communicated with a second central through hole which is axially arranged; the bottom cover is sealed on the bottom surface of the liquid storage pipe body, the outer side end of each S-shaped micro-pipeline is communicated with the bottom end of the corresponding liquid storage cavity, and the bottom cover is used for preventing polymerase liquid in the corresponding liquid storage cavity from being mixed with liquid in the reaction tank by using air in the S-shaped micro-pipeline under the action of non-external force.
The S-shaped micro-pipeline type nucleic acid amplification closed reaction tube capable of being preloaded with reagents comprises: the liquid storage pipe body is cylindrical, the liquid storage tank is in an inverted conical space, the top surface of the liquid storage tank is open, and the liquid storage tank is positioned at the upper part of the liquid storage pipe body; the small end of the inverted conical liquid storage tank is communicated with the bottom surface of the liquid storage pipe body through the first central through hole; the liquid storage cavity is cylindrical space and bottom surface opening, and is located the well lower part of liquid storage body, and all liquid storage cavity equipartitions are in around first central through-hole.
The S-shaped micro-pipeline type nucleic acid amplification closed reaction tube capable of being preloaded with reagents comprises: the bottom cover is in a wafer shape, the outer diameter of the bottom cover is the same as that of the liquid storage pipe body, all the S-shaped micro-pipelines are positioned on the same end face of the bottom cover in a groove mode, and all the S-shaped micro-pipelines are uniformly distributed around the second central through hole; the inner side end of each S-shaped micro pipeline is communicated with the second central through hole, and the outer side end of each S-shaped micro pipeline bends and extends outwards along the radial direction of the bottom cover.
The S-shaped micro-pipeline type nucleic acid amplification closed reaction tube capable of being preloaded with reagents comprises: the upper half part of the reaction tube body is cylindrical, and the lower half part of the reaction tube body is in an inverted cone shape; the reaction tank is positioned at the lower part of the reaction tube body and is in an inverted conical space; the top of the reaction tank extends upwards to form a cylindrical space, the top surface of the reaction tank is provided with an opening, and the reaction tank is used for being matched with a liquid storage pipe body; the top edge of the cylindrical reaction tube body extends outwards along the radial direction to form a circle of first steps.
The S-shaped micro-pipeline type nucleic acid amplification closed reaction tube capable of being preloaded with reagents comprises: the sealing cover body is cylindrical, and a circle of second step matched with the first step extends outwards from the top edge of the sealing cover body along the radial direction; the outer diameter of the sealing cover body is larger than the inner diameter of the cylindrical space at the upper part of the reaction tube body.
The S-shaped micro-pipeline type nucleic acid amplification closed reaction tube capable of being preloaded with reagents comprises: the top surface of the sealing cover body is also provided with a puncture hole with the aperture larger than the diameter of the syringe needle.
The S-shaped micro-pipeline type nucleic acid amplification closed reaction tube capable of being preloaded with reagents comprises: the inner wall of the reaction tube body close to the opening is provided with four convex clamping tables which are centrosymmetric in a plane vertical to the axial lead of the reaction tube body; correspondingly, four concave first passing grooves are uniformly distributed on the outer wall of the liquid storage pipe body, and the four first passing grooves are all parallel to the axial lead of the liquid storage pipe body; four concave second passing grooves are uniformly distributed on the outer wall of the bottom cover and are parallel to the axial lead of the bottom cover; four reverse L-shaped clamping grooves are uniformly distributed on the outer wall of the sealing cover body, and each clamping groove is formed by connecting a section of longitudinal clamping groove and a section of transverse clamping groove.
The S-shaped micro-pipeline type nucleic acid amplification closed reaction tube capable of being preloaded with reagents comprises: the middle position of the transverse clamping groove is also provided with a convex locking platform, and one side of the locking platform facing the longitudinal clamping groove is an inclined plane.
The S-shaped micro-pipeline type nucleic acid amplification closed reaction tube capable of being preloaded with reagents comprises: the S-shaped micro-pipeline is in an S-shaped curve shape, the cross section of the S-shaped micro-pipeline is in a semicircular shape, the diameter of the S-shaped micro-pipeline is 0.1-1.0 mm, and meanwhile the length of the S-shaped micro-pipeline is more than 3 mm.
The S-shaped micro-pipeline nucleic acid amplification closed reaction tube capable of pre-loading the reagent provided by the utility model adopts the liquid storage tube body with a plurality of liquid storage cavities and the bottom plate with the transverse S-shaped micro-pipeline, and utilizes the air physical zone inside the S-shaped micro-pipeline to separate the liquid in the liquid storage cavities and the liquid in the reaction tank, so that a user does not need to additionally prepare any reagent, thereby greatly simplifying the operation link; the sealing cover body does not need to be opened in the whole operation process, so that complete isolation from the outside is thoroughly realized, and the possibility of reagent pollution by aerosol is effectively avoided; in addition, when the preassembly of various reagents is realized, the stability and the validity period of the various preassembly reagents cannot be changed, and the reaction tube body, the bottom cover, the liquid storage tube body and the sealing cover body can be produced in a large scale through an injection molding process, so that the application cost is obviously reduced, the reaction tube body is particularly suitable for multienzyme reaction, and the reaction tube body has an obvious practical value.
Drawings
FIG. 1 is an exploded view of an embodiment of the S-shaped micro-channel type nucleic acid amplification closed reaction tube of the present invention, which can be pre-loaded with reagents;
FIG. 2 is an enlarged perspective view (with a cross section) of an embodiment of the S-shaped micro-channel type nucleic acid amplification closed reaction tube of the present invention, which can be pre-loaded with reagents;
FIG. 3 is an enlarged front view of an embodiment of the S-shaped micro-channel type nucleic acid amplification closed reaction tube of the present invention, which can be pre-loaded with reagents;
FIG. 4 is a bottom view and a top view of a liquid storage tube, a front view of a sealing cap, and a top view of a reaction tube, according to an embodiment of the S-shaped micro-channel type nucleic acid amplification closed reaction tube pre-loaded with a reagent according to the present invention;
FIG. 5 is a top view of a bottom cap used in an embodiment of the S-shaped micro-channel type nucleic acid amplification closed reaction tube of the present invention, which can be pre-loaded with a reagent.
Summary of the numbers in the figures: the reaction tube 400, the reaction tank 410, the first step 420, the clamping table 430, the bottom cover 500, the second central through hole 510, the second passing groove 520, the S-shaped micro-pipe 530, the liquid storage tube 600, the liquid storage tank 610, the first central through hole 611, the first passing groove 620, the liquid storage cavity 630, the sealing cover 700, the second step 710, the clamping groove 720, the longitudinal clamping groove 721, the transverse clamping groove 722, and the locking table 730.
Detailed Description
The following detailed description and examples of the present invention are provided in connection with the accompanying drawings, which are set forth for the purpose of illustration only and are not intended to limit the invention.
As shown in FIGS. 1 and 2, FIG. 1 is an exploded view of an embodiment of an S-shaped micro-channel type nucleic acid amplification closed reaction tube capable of being preloaded with a reagent according to the present invention, and FIG. 2 is an enlarged perspective view (with a section) of an embodiment of an S-shaped micro-channel type nucleic acid amplification closed reaction tube capable of being preloaded with a reagent according to the present invention; the S-shaped micro-pipeline type nucleic acid amplification closed reaction tube comprises a reaction tube body 400, a bottom cover 500, a liquid storage tube body 600 and a sealing cover body 700; the bottom cover 500 and the liquid storage tube body 600 are both installed in the reaction tube body 400, and the sealing cover body 700 seals the mouth of the reaction tube body 400; wherein,
the reaction tube 400 is provided with a reaction tank 410 for containing reaction liquid, and the outer wall of the sealing cover 700 is tightly matched with the inner wall of the reaction tube 400 to prevent the liquid in the reaction tank 410 from flowing out of the mouth of the reaction tube 400;
the upper portion of the liquid storage tube 600 is provided with a liquid storage tank 610 communicated with the upper end surface thereof for containing a nucleic acid liquid sample to be detected before detection, the bottom of the liquid storage tank 610 is communicated with the reaction tank 410 of the reaction tube 400 through a first central through hole 611 arranged axially, after assembly, the lower end surface of the sealing cover body 700 is in close contact with the upper end surface of the liquid storage tube 600 to prevent the liquid in the liquid storage tank 610 from flowing out of the upper portion of the liquid storage tube 600;
the liquid storage tube body 600 is at least internally provided with two liquid storage cavities 630 communicated with the lower end face of the liquid storage tube body and used for containing at least two different types of polymerase liquid;
at least two S-shaped micro-pipes 530 are transversely arranged on the upper end surface of the bottom cover 500, and the inner side ends of all the S-shaped micro-pipes 530 are communicated with a second central through hole 510 which is axially arranged;
the bottom cover 500 is sealed on the bottom surface of the liquid storage tube 600, the outer end of each S-shaped micro-tube 530 is communicated with the bottom end of the corresponding liquid storage cavity 630 of the liquid storage tube 600, and is used for preventing or isolating the polymerase liquid in the corresponding liquid storage cavity 630 from being mixed with the reaction liquid or the premixed liquid in the reaction tank 410 by using the air in the S-shaped micro-tube 530 under the non-external force action; external forces in this context refer to external forces such as jerks, falls or centrifugation with a certain degree of acceleration.
Under the normal condition without external force such as fling, falling or centrifugation, even if the S-shaped micro-channel nucleic acid amplification closed reaction tube is vertically arranged like fig. 2, the polymerase liquid in each liquid storage cavity 630 does not enter the reaction tank 410 due to the air isolation function in each S-shaped micro-channel 530 and the surface tension function of the polymerase liquid; similarly, even if the S-shaped micro-tube type nucleic acid amplification closed reaction tube is placed upside down, due to the air isolation effect in the S-shaped micro-tube 530 and the surface tension effect of the polymerase liquid, the reaction liquid in the reaction tank 410 will enter the liquid storage tank 610 of the liquid storage tube body 600 through the second central through hole 510 and the first central through hole 611, but will not enter each liquid storage cavity 630;
the nucleic acid liquid to be detected which is added into the liquid storage tank 610 by piercing the sealing cover body 700 before detection enters the reaction tank 410 through the first central through hole 611 and the second central through hole 510 and is premixed with the reaction liquid in the reaction tank 410; under the action of an external force with a specific acceleration, the S-shaped micro-tube 530 with a specific shape and size, the different kinds of polymerase liquid in each liquid storage chamber 630 overcome the air isolation and liquid surface tension, enter the reaction chamber 410 through the second central through hole 510, and are mixed with the premixed liquid in the reaction chamber 410 for the second time.
The S-shaped micro-pipeline nucleic acid amplification closed reaction tube which can be pre-filled with reagents of the utility model can firstly reverse the liquid storage tube body 600, so that the mouth part of the liquid storage cavity 630 on the liquid storage tube body is upward, and different types of polymerase liquid are added into each liquid storage cavity 630; then, one surface of the bottom cover 500 with the S-shaped micro-pipes 530 faces downwards and is placed on the liquid storage pipe body 600, and the outer side end of each S-shaped micro-pipe 530 is ensured to be communicated with the corresponding liquid storage cavity 630 on the liquid storage pipe body 600; and then, the bottom cover 500 and the liquid storage tube body 600 are hermetically connected into a whole in a hot melting or ultrasonic processing mode for use in detection.
Then, adding a reaction liquid into the reaction tank 410 of the reaction tube 400, then enabling the opening of the liquid storage tank 610 of the liquid storage tube 600 to face upwards, placing the liquid storage tube 600 and the bottom cover 500 which are sealed together, then plugging the sealing cover body 700, and discharging air with a corresponding volume at the upper part of the reaction tube 400 when plugging the sealing cover body 700; since the liquid storage tank 610 is not filled with the nucleic acid liquid to be tested, and the different kinds of polymerase liquid in each liquid storage chamber 630 are not mixed with the reaction liquid in the reaction tank 410, the shelf life and stability of the reaction liquid in the reaction tank 410 are not affected, and the shelf life and stability of the different kinds of polymerase liquid in each liquid storage chamber 630 are not affected.
Before the nucleic acid amplification detection, the sealing cover body 700 is punctured by using a needle of the injector, and the nucleic acid liquid sample to be detected is injected into the liquid storage tank 610 of the liquid storage tube body 600, so that the pollution of aerosol in the environment due to the opening of the sealing cover body 700 can be avoided; after the pre-mixing, the polymerase solution is placed into a centrifugal device, and under the centrifugal force of a certain degree of acceleration, the different kinds of polymerase solution in each solution storage cavity 630 enter the reaction tank 410 of the reaction tube 400 to be mixed with the pre-mixing solution for a second time.
In the whole operation process, a user does not need to prepare any reagent additionally, the operation link is greatly simplified, the sealing cover body 700 does not need to be opened in the process of mixing twice, the sealing cover body is completely isolated from the external environment, and the possibility of being polluted by aerosol is effectively avoided.
In addition, because the S type micro-pipeline 530 that has the air in it carries out the physics district and separates, when having realized the reagent pre-installation, still can not cause the stability and the validity period of pre-installation reagent to change, and reaction body 400, bottom 500, stock solution body 600 and seal cover body 700 can both realize mass production through injection moulding process again, wherein, reaction body 400, bottom 500 and stock solution body 600 all can adopt the polypropylene material through plastic mould injection moulding, and seal cover body 700 can adopt medical rubber through plastic mould injection moulding, this is showing and has reduced the use cost, compare the higher freeze-drying process with the technology unstability of cost, the utility model discloses the S type micro-pipeline formula nucleic acid amplification closed reaction tube that can pre-install reagent has obvious practical value.
Referring to FIG. 3, FIG. 3 is an enlarged front view of an embodiment of the S-shaped micro-channel type nucleic acid amplification closed reaction tube of the present invention, which can be pre-loaded with reagents; preferably, the liquid storage tube 600 is cylindrical, and the liquid storage tank 610 is an inverted conical space with an open top surface and is located at the upper part of the liquid storage tube 600; the small end of the inverted conical liquid storage tank 610 is communicated with the bottom surface of the liquid storage pipe body 600 through the first central through hole 611; the liquid storage cavity 630 is a cylindrical space with an opening on the bottom surface and is located at the middle lower part of the liquid storage tube body 600.
Preferably, the bottom cap 500 is in a disc shape, the outer diameter of the bottom cap is the same as the outer diameter of the liquid storage tube 600, and all the S-shaped micro-tubes 530 are located on the same end surface of the bottom cap 500 in a groove form.
Preferably, the upper half of the reaction tube 400 is cylindrical, and the lower half is in the shape of an inverted cone; the reaction tank 410 is located at the lower part of the reaction tube 400 and has an inverted conical space; the top of the reaction chamber 410 extends upwards to form a cylindrical space with an opening on the top surface for accommodating the liquid storage tube body 500 and the bottom cover 500; the top edge of the cylindrical reaction tube 400 extends radially outward to form a circle of first step 420, which is convenient for being placed into a centrifuge for fixing.
Preferably, the sealing cap body 700 is also cylindrical, and a second step 710 matched with the first step 420 of the reaction tube 400 extends radially outwards from the top edge of the sealing cap body 700; the outer diameter of the sealing cap body 700 is slightly larger than the inner diameter of the cylindrical space at the upper portion of the reaction tube body 400, so as to ensure the sealing performance of the sealing cap body 700 to the mouth portion of the reaction tube body 400.
Preferably, the top surface of the sealing cover body 700 may be further provided with a piercing hole (not shown) having a diameter larger than that of the syringe needle, so as to facilitate the syringe needle to pierce the sealing cover body 700.
Referring to FIGS. 4 and 1, FIG. 4 is a front view of a sealing cap and a top view of a liquid storage tube and a reaction tube for an embodiment of an S-shaped micro-channel nucleic acid amplification closed reaction tube pre-filled with reagents according to the present invention, wherein (a) is a top view of the reaction tube, (b) is a top view of the liquid storage tube, (c) is a bottom view of the liquid storage tube, and (d) is a front view of the sealing cap; further, four protruded clamping platforms 430 (shown in fig. 1) are disposed on the inner wall of the reaction tube 400 near the mouth, and the four clamping platforms 430 are in central symmetry in a plane perpendicular to the axial line of the reaction tube 400; correspondingly, four concave first passing grooves 620 (shown in fig. 1) are uniformly distributed on the outer wall of the liquid storage tube body 600, and the four first passing grooves 620 are all parallel to the axial lead of the liquid storage tube body 600, so that when the liquid storage tube body 600 is placed in the reaction tube body 400, the corresponding clamping platforms 430 on the reaction tube body 400 can pass through smoothly; four inverted-L-shaped slots 720 (shown in fig. 1) are uniformly distributed on the outer wall of the sealing cover body 700, and each slot 720 is formed by connecting a section of longitudinal slot 721 and a section of transverse slot 722, so that when the sealing cover body 700 is plugged and rotated, the corresponding clamping table 430 on the reaction tube body 400 can smoothly pass through the longitudinal slot 721 and be clamped at the tail end of the transverse slot 722; therefore, the sealing cover body 700 is tightly buckled on the reaction tube body 400, the locking structure is more convenient and quicker to operate than a thread structure, and the locking of the sealing cover body 700 and the reaction tube body 400 can be realized only by plugging and rotating.
Further, a convex locking stage 730 (shown in fig. 1) is further disposed at the middle position of the transverse slot 722, and a side of the locking stage 730 facing the longitudinal slot 721 is an inclined surface, so that the resistance of the locking stage 430 being locked into the end of the transverse slot 722 when the sealing cover body 700 is rotated can be greatly reduced, and the locking stage 430 is prevented from moving reversely in the transverse slot 722 after the sealing cover body 700 is rotated, so as to lock the sealing cover body 700 and prevent the sealing cover body 700 from being removed from the reaction tube 400.
In the embodiment of the S-shaped micro-channel nucleic acid amplification closed reaction tube capable of being pre-loaded with reagents of the present invention, specifically, taking the example of being able to contain four different types of polymerase liquids, as can be seen from fig. 3 and fig. 4(c), four liquid storage chambers 630 are uniformly distributed around the first central through hole 611; correspondingly, as shown in fig. 5, fig. 5 is a top view of a bottom cover used in an embodiment of the S-shaped micro-channel type nucleic acid amplification closed reaction tube of the present invention, wherein the reagent can be pre-loaded, and four S-shaped micro-channels 530 are uniformly distributed around the second central through hole 510; the inner end of each S-shaped micro-pipe 530 is communicated with the second central through hole 510, and the outer end is bent and extended outward along the radial direction of the bottom cover 500;
meanwhile, four concave second passing grooves 520 (shown in fig. 1) are uniformly distributed on the outer wall of the bottom cover 500, and the four second passing grooves 520 are parallel to the axial line of the bottom cover 500, so that the corresponding clamping platforms 430 on the reaction tube 400 can pass through smoothly when the bottom cover 500 is placed in the reaction tube.
Specifically, the S-shaped micro-tube 530 is in an S-shaped curve shape, the cross section of the S-shaped micro-tube 530 is in a semicircular shape or a rectangular shape, the diameter of the semicircular shape is 0.1-1.0 mm, and the length of the S-shaped micro-tube 530 is more than 3mm, for example, the length of the S-shaped micro-tube 530 is 0.5mm and 5mm, so that the polymerase liquid in the liquid storage cavity 630 can be separated from the reaction liquid or the premixed liquid in the reaction tank 410 by air under the non-external force, and the polymerase liquid in the liquid storage cavity 630 can enter the reaction tank 410 through the S-shaped micro-tube 530 and can be mixed with the reaction liquid or the premixed liquid in the reaction tank 410 under the centrifugal force of 1.0-2.0 KG.
It should be understood that the above-mentioned embodiments are only preferred embodiments of the present invention, and are not intended to limit the technical solutions of the present invention, and those skilled in the art can add, subtract, replace, change or modify the above-mentioned embodiments within the spirit and principle of the present invention, and all such additions, substitutions, changes or modifications should fall within the scope of the appended claims.
Claims (9)
1. An S-shaped micro-pipeline type nucleic acid amplification closed reaction tube capable of being preloaded with reagents is characterized in that: comprises a reaction tube body, a liquid storage tube body, a bottom cover and a sealing cover body; the bottom cover and the liquid storage tube body are both arranged in the reaction tube body, and the sealing cover body is plugged at the opening of the reaction tube body; wherein,
the reaction tube body is internally provided with a reaction tank for containing reaction liquid, and the outer wall of the sealing cover body is tightly matched with the inner wall of the reaction tube body;
the upper part of the liquid storage tube body is provided with a liquid storage tank communicated with the upper end surface of the liquid storage tube body and used for injecting nucleic acid liquid to be detected before detection, the bottom of the liquid storage tank is communicated with the reaction tank through a first central through hole which is axially arranged, and the lower end surface of the sealing cover body is tightly contacted with the upper end surface of the liquid storage tube body after assembly; the liquid storage tube body is internally provided with at least two liquid storage cavities communicated with the lower end surface of the liquid storage tube body and used for containing at least two different types of polymerase liquid;
the upper end face of the bottom cover is transversely provided with at least two S-shaped micro-pipelines, and the inner side ends of all the S-shaped micro-pipelines are communicated with a second central through hole which is axially arranged;
the bottom cover is sealed on the bottom surface of the liquid storage pipe body, the outer side end of each S-shaped micro-pipeline is communicated with the bottom end of the corresponding liquid storage cavity, and the bottom cover is used for preventing polymerase liquid in the corresponding liquid storage cavity from being mixed with liquid in the reaction tank by using air in the S-shaped micro-pipeline under the action of non-external force.
2. The S-shaped micro-channel nucleic acid amplification closed reaction tube capable of being preloaded with reagents according to claim 1, wherein: the liquid storage pipe body is cylindrical, the liquid storage tank is in an inverted conical space, the top surface of the liquid storage tank is open, and the liquid storage tank is positioned at the upper part of the liquid storage pipe body; the small end of the inverted conical liquid storage tank is communicated with the bottom surface of the liquid storage pipe body through the first central through hole; the liquid storage cavity is cylindrical space and bottom surface opening, and is located the well lower part of liquid storage body, and all liquid storage cavity equipartitions are in around first central through-hole.
3. The S-shaped micro-channel nucleic acid amplification closed reaction tube capable of being preloaded with reagents according to claim 1, wherein: the bottom cover is in a wafer shape, the outer diameter of the bottom cover is the same as that of the liquid storage pipe body, all the S-shaped micro-pipelines are positioned on the same end face of the bottom cover in a groove mode, and all the S-shaped micro-pipelines are uniformly distributed around the second central through hole; the inner side end of each S-shaped micro pipeline is communicated with the second central through hole, and the outer side end of each S-shaped micro pipeline bends and extends outwards along the radial direction of the bottom cover.
4. The S-shaped micro-channel nucleic acid amplification closed reaction tube capable of being preloaded with reagents according to claim 1, wherein: the upper half part of the reaction tube body is cylindrical, and the lower half part of the reaction tube body is in an inverted cone shape; the reaction tank is positioned at the lower part of the reaction tube body and is in an inverted conical space; the top of the reaction tank extends upwards to form a cylindrical space, the top surface of the reaction tank is provided with an opening, and the reaction tank is used for being matched with a liquid storage pipe body; the top edge of the cylindrical reaction tube body extends outwards along the radial direction to form a circle of first steps.
5. The S-shaped micro-channel nucleic acid amplification closed reaction tube capable of being preloaded with reagents according to claim 4, wherein: the sealing cover body is cylindrical, and a circle of second step matched with the first step extends outwards from the top edge of the sealing cover body along the radial direction; the outer diameter of the sealing cover body is larger than the inner diameter of the cylindrical space at the upper part of the reaction tube body.
6. The S-shaped micro-channel nucleic acid amplification closed reaction tube capable of being preloaded with reagents according to claim 1, wherein: the top surface of the sealing cover body is also provided with a puncture hole with the aperture larger than the diameter of the syringe needle.
7. The S-shaped micro-channel nucleic acid amplification closed reaction tube capable of being preloaded with reagents according to claim 1, wherein: the inner wall of the reaction tube body close to the opening is provided with four convex clamping tables which are centrosymmetric in a plane vertical to the axial lead of the reaction tube body; correspondingly, four concave first passing grooves are uniformly distributed on the outer wall of the liquid storage pipe body, and the four first passing grooves are all parallel to the axial lead of the liquid storage pipe body; four concave second passing grooves are uniformly distributed on the outer wall of the bottom cover and are parallel to the axial lead of the bottom cover; four reverse L-shaped clamping grooves are uniformly distributed on the outer wall of the sealing cover body, and each clamping groove is formed by connecting a section of longitudinal clamping groove and a section of transverse clamping groove.
8. The reagent-prefillable S-shaped micro-channel nucleic acid amplification closed reaction tube according to claim 7, wherein: the middle position of the transverse clamping groove is also provided with a convex locking platform, and one side of the locking platform facing the longitudinal clamping groove is an inclined plane.
9. The reagent-prefillable S-shaped micro-channel nucleic acid amplification closed reaction tube according to any one of claims 1 to 8, wherein: the S-shaped micro-pipeline is in an S-shaped curve shape, the cross section of the S-shaped micro-pipeline is in a semicircular shape, the diameter of the S-shaped micro-pipeline is 0.1-1.0 mm, and meanwhile the length of the S-shaped micro-pipeline is more than 3 mm.
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| CN201820837403.5U CN208395201U (en) | 2018-06-01 | 2018-06-01 | It is a kind of can pre-filled reagent S type microchannel formula nucleic acid amplification airtight reactor tube |
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| CN201820837403.5U CN208395201U (en) | 2018-06-01 | 2018-06-01 | It is a kind of can pre-filled reagent S type microchannel formula nucleic acid amplification airtight reactor tube |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642146A (en) * | 2018-06-01 | 2018-10-12 | 广州双螺旋基因技术有限公司 | It is a kind of can pre-filled reagent S type microchannel formula nucleic acid amplification airtight reactor tubes |
| CN112760208A (en) * | 2020-12-31 | 2021-05-07 | 苏州安基生物科技有限公司 | Constant-temperature PCR reaction tube and use method thereof |
| WO2021164088A1 (en) * | 2020-02-17 | 2021-08-26 | 杭州杰毅生物技术有限公司 | Closed consumable and test kit |
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2018
- 2018-06-01 CN CN201820837403.5U patent/CN208395201U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642146A (en) * | 2018-06-01 | 2018-10-12 | 广州双螺旋基因技术有限公司 | It is a kind of can pre-filled reagent S type microchannel formula nucleic acid amplification airtight reactor tubes |
| CN108642146B (en) * | 2018-06-01 | 2024-02-02 | 广州双螺旋基因技术有限公司 | S-type microtube nucleic acid amplification closed reaction tube capable of being preloaded with reagent |
| WO2021164088A1 (en) * | 2020-02-17 | 2021-08-26 | 杭州杰毅生物技术有限公司 | Closed consumable and test kit |
| CN112760208A (en) * | 2020-12-31 | 2021-05-07 | 苏州安基生物科技有限公司 | Constant-temperature PCR reaction tube and use method thereof |
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