CN218507779U - Cervical cancer gene methylation detection device - Google Patents
Cervical cancer gene methylation detection device Download PDFInfo
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- CN218507779U CN218507779U CN202222357112.5U CN202222357112U CN218507779U CN 218507779 U CN218507779 U CN 218507779U CN 202222357112 U CN202222357112 U CN 202222357112U CN 218507779 U CN218507779 U CN 218507779U
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Abstract
The utility model discloses a methylation detection device for cervical cancer genes, which comprises a reagent strip, wherein the reagent strip is provided with at least nine cavities which are not communicated with each other, and the top of each cavity is provided with a sealing structure for sealing all the cavities; the cavity comprises a cracking cavity, a magnetic bead cavity preloaded with magnetic beads, a magnetic sleeve cavity preloaded with a magnetic sleeve, a conversion cavity, a desulfonation cavity, a washing cavity, an extrusion cavity preloaded with a rubber plug, a tube cover cavity preloaded with a tube cover and an elution cavity; a reagent tube is arranged in the conversion cavity, and conversion binding liquid is pre-filled in the reagent tube; a reaction tube is fixed at the bottom of the reagent strip, the reaction tube is communicated with through holes at the bottoms of the elution cavity and the extrusion cavity, and PCR reaction liquid is pre-filled in the reaction tube; except the magnetic bead cavity, the magnetic sleeve cavity, the extrusion cavity and the tube cover cavity, other cavities are respectively preloaded with different reagents for sample processing. The utility model discloses simple structure, it is with low costs, but disposable can be further applied to clinically providing wide prospect for cervical carcinoma gene methylation detection technology.
Description
Technical Field
The utility model relates to a detect technical field, especially relate to a cervical carcinoma gene methylation detection device.
Background
Cancer gene detection is a worldwide trend, early diagnosis of the risk and occurrence of cancer, early intervention and intervention can greatly improve the 5-year survival rate of cancer and reduce the mortality rate. Worldwide epigenetic research has become an important history of the most advanced early diagnosis of cancer: one of the characteristics of the tumor is methylation imbalance, namely, the methylation degree of a specific cancer suppressor gene at a canceration part is obviously increased, the expression is reduced or even silenced, the cancer suppressor function is lost, and finally, the cancer is rapidly developed. Therefore, the methylation state of a specific gene can be taken as an important index of the occurrence and development of tumors.
In the development of cervical cancer, cervical intraepithelial lesions (CIN) are classified into three classes, CIN1, CIN2 and CIN3. The cervical cancer development process is accompanied by abnormal expression of specific gene methylation, and the detection of the gene methylation degree can be used as an important index of cervical canceration. When detecting a transforming lesion (part of CIN2 and CIN 3) by gene methylation, the risk of cancer progression can be known, and the doctor is prompted whether to immediately perform therapeutic intervention or keep track of observation. The methylation detection step of the cervical cancer gene comprises sample cracking, nucleic acid sulfite conversion, nucleic acid purification and fluorescence PCR detection, and the whole experiment has the disadvantages of multiple steps, long time consumption, complex operation, manual operation and easy experiment error. In order to reduce the operation error and reduce the labor cost, an integrated full-automatic detection device integrating cervical cancer sample lysis, nucleic acid sulfite conversion, nucleic acid purification and fluorescence PCR detection is urgently needed.
SUMMERY OF THE UTILITY MODEL
The utility model provides a cervical carcinoma gene methylation detection device, the device simple structure, it is with low costs, but disposable detects cervical carcinoma gene fast effectively, and has avoided the cross contamination between the sample, and the security is high, further is applied to clinically providing wide prospect for cervical carcinoma gene methylation detection technology.
A cervical cancer gene methylation detection device comprises a reagent strip, wherein the reagent strip is provided with at least nine cavities which are not communicated with each other, and a sealing structure is arranged at the top of each cavity to seal all the cavities; the cavity comprises a cracking cavity for cracking a sample, a magnetic bead cavity preloaded with magnetic beads, a magnetic sleeve cavity preloaded with a magnetic sleeve, a conversion cavity for conversion, a desulfonation cavity for desulfonation reaction, a washing cavity for washing, an extrusion cavity preloaded with a rubber plug, a tube cover cavity preloaded with a tube cover and an elution cavity for elution;
a reagent tube is arranged in the conversion cavity, and conversion binding liquid is pre-filled in the reagent tube;
a reaction tube is fixed at the bottom of the reagent strip, the reaction tube is communicated with through holes at the bottoms of the elution cavity and the extrusion cavity, and PCR reaction liquid is pre-filled in the reaction tube;
except the magnetic bead cavity, the magnetic sleeve cavity, the extrusion cavity and the tube cover cavity, other cavities are respectively preloaded with different reagents for sample processing.
The utility model discloses with required reagent of cervical carcinoma gene methylation detection and utensil set in a reagent strip, through this reagent strip, can realize examining cervical carcinoma gene fast in detecting instrument, convenient and fast, and disposable, easy operation further is applied to clinically providing wide prospect for cervical carcinoma gene methylation detection technique.
When the detection is carried out, the magnetic sleeve can be moved back and forth and up and down to a required position through an external detection instrument, the magnetic beads are moved into different cavities, so that reagents in the cavities are uniformly mixed by continuously inserting the magnetic sleeve into the corresponding cavity to move the magnetic beads and then rotating, steps of cracking, adsorption, conversion and combination, desulfonation, washing, elution and the like are realized, and finally, a target object is transferred into the reaction tube to carry out PCR detection.
Further, the rubber buffer is cylindrical, with the shape adaptation in extrusion chamber, the bottom of rubber buffer is equipped with the opening, and inside cavity, top are for pressing the contact surface.
Generally, the nucleic acid is eluted in the elution cavity at last, so that the target object exists in the mixed solution in the elution cavity, at the moment, because the through holes communicated with the elution cavity of the reaction tube are generally few, and the speed of flowing into the reaction tube is slow, the rubber plug needs to be extruded through the bottom of the magnetic sleeve, so that the air in the rubber plug is extruded into the reaction tube through the through holes at the bottom of the extrusion cavity, and then is discharged from the elution cavity through the through holes communicated with the elution cavity of the reaction tube, and then the magnetic sleeve is taken out.
Further, the tube cover is cylindrical and is matched with the elution cavity in shape, and the tube cover is a plastic cover.
At the aforesaid through extrusion rubber buffer to make the mixed liquid of elution intracavity accelerate to flow into the in-process of reaction tube, after taking out the magnetic sleeve, detecting instrument takes out the tube cap of tube cap intracavity, and removes the tube cap to the elution intracavity, be used for blockking up sealedly to the elution chamber, avoid receiving external influence in the testing process.
Further, the bottom slope of elution chamber sets up, and the through-hole has been seted up to the lower extreme, through the through-hole with the reaction tube intercommunication.
The bottom of slope setting can accelerate the mixed liquid to flow into in the reaction tube through the through-hole of elution chamber bottom, and under the slope effect, when the intraductal pressure reduction of reaction, probably form swirl effect to reach the effect of accelerating the inflow.
Furthermore, the reagent tube is fixed on one side in the conversion cavity, an inclined plane is arranged at the corresponding position of the bottom of the reagent tube in the conversion cavity, and the bottom of the reagent tube is of a thin film structure which is easy to pierce.
When the transformation is carried out, the magnetic sleeve is extended into the reagent tube, so that the thin film structure at the bottom of the reagent tube can be easily punctured, the transformation binding liquid in the reagent tube can quickly flow down through the inclined surface to be mixed with the transformation liquid in the transformation cavity, and the transformation of the nucleic acid is finished.
Furthermore, four washing cavities are arranged, and washing liquids with different concentrations are pre-filled in the four washing cavities respectively. Through containing four washing chamber from high to low washing liquid concentration, can effectively carry out washing operation to the magnetic bead.
Furthermore, the magnetic sleeve is in a hollow cylindrical tube shape, the bottom end of the magnetic sleeve is in a closed conical shape, and the top end of the magnetic sleeve is open.
The magnetic rod of the detection instrument can be inserted into the magnetic sleeve through the opening, so that the magnetic sleeve is provided with magnetism to adsorb magnetic beads in the reagent. At the same time, the conical shape of the bottom end of the magnetic sleeve can be conveniently used to pierce some membranes.
Further, the sealing structure is a sealing film, and the sealing film is sealed through heat sealing or ultrasonic welding.
The sealing structure is closely contacted with the top of the reagent strip to play a role of sealing the cavity, so that the reagent in the cavity is limited in the cavity.
Furthermore, the bottoms or/and the outer walls of the cracking cavity and the conversion cavity are/is externally connected to a heating module of the detection instrument, and the heating module is used for heating the cavity, so that the cracking and the conversion of the sample are further accelerated.
Furthermore, the bottom of the reaction tube is externally connected with a detection instrument, and the detection instrument is used for carrying out PCR reaction and detection on the reaction tube.
The utility model has the advantages as follows:
the utility model discloses with cervical sample schizolysis, nucleic acid conversion, nucleic acid purification, fluorescence PCR detection integration in an integral type detection device for cervical cancer gene methylation detects can full-automatic completion, need not manual sample pretreatment, has reduced operating error, has reduced the human cost. The utility model discloses a detection device has greatly simplified overall structure, and disposable can avoid the cross contamination between the sample to improve the sample and draw and the accuracy that detects, only need one step of application of sample operation, convenient to use further is applied to clinically providing wide prospect for cervical carcinoma gene methylation detection technology.
Drawings
FIG. 1 is a schematic structural diagram of the cervical cancer gene methylation detection device of the present invention;
FIG. 2 is an internal schematic view of the cervical cancer gene methylation detection apparatus of the present invention;
fig. 3 is an enlarged schematic view of a portion a of fig. 2.
In the figure: the device comprises a reagent strip 1, a lysis cavity 101, a magnetic bead cavity 102, a magnetic sleeve cavity 103, a washing cavity 104, a conversion cavity 105, a desulfonation cavity 106, a tube cover cavity 107, an extrusion cavity 108, an elution cavity 109, magnetic beads 2, a magnetic sleeve 3, a tube cover 4, a rubber plug 5, a reagent tube 6 and a reaction tube 7.
Detailed Description
The drawings are for illustrative purposes only and are not to be construed as limiting the patent; for the purpose of better illustrating the present embodiments, certain elements of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationships depicted in the drawings are for illustrative purposes only and are not to be construed as limiting the present patent.
Example 1:
as shown in fig. 1-3, a methylation detection device for cervical cancer genes comprises a reagent strip 1, wherein the reagent strip 1 is made of semitransparent materials, thirteen cavities which are arranged side by side and are not communicated with each other are arranged on the reagent strip 1, and a sealing film (not shown in the figure) is arranged at the top of each cavity to seal all the cavities; wherein the cavities from left to right are respectively: a lysis chamber 101 for lysing a sample, a magnetic bead chamber 102 pre-filled with magnetic beads 2, a magnetic sleeve chamber 103 pre-filled with a magnetic sleeve 3, a magnetic bead chamber 102 pre-filled with magnetic beads 2, a washing chamber 104 for washing, a conversion chamber 105 for conversion, a desulfonation chamber 106 for desulfonation, three washing chambers 104 which are sequentially arranged side by side and pre-filled with washing solutions with different concentrations, a tube cover chamber 107 pre-filled with a tube cover 4, an extrusion chamber 108 pre-filled with a rubber plug 5, and an elution chamber 109 for elution;
a reagent tube 6 is arranged in the conversion cavity 105, and conversion binding liquid is pre-filled in the reagent tube 6;
a reaction tube 7 is fixed at the bottom of the reagent strip 1, the reaction tube 7 is communicated with through holes at the bottoms of the elution cavity 109 and the extrusion cavity 108 (see fig. 3), a PCR reaction solution is pre-filled in the reaction tube 7, and the bottom of the reaction tube 7 is externally connected to a detection instrument (not shown in the figure) which is used for carrying out PCR reaction and detection on the reaction tube 7;
in addition to the magnetic bead chamber 102, the magnetic sleeve chamber 103, the extrusion chamber 108, and the cap chamber 107, the other chambers are filled with different reagents for sample processing.
The utility model discloses with required reagent of cervical carcinoma gene methylation detection and utensil set in a reagent strip 1, through this reagent strip 1, can realize examining cervical carcinoma gene fast in detecting instrument, convenient and fast, and disposable, easy operation further is applied to clinically providing wide prospect for cervical carcinoma gene methylation detection technique.
When detection is carried out, the magnetic sleeve 3 can be moved back and forth and up and down to a required position through an external detection instrument, the magnetic beads 2 are moved into different cavities, so that reagents in the cavities are uniformly mixed by continuously inserting the magnetic sleeve 3 into the corresponding cavity to move the magnetic beads 2 and then rotating, steps of cracking, adsorption, conversion and combination, desulfation, washing, elution and the like are realized, and finally, a target object is transferred into the reaction tube 7 to be subjected to PCR detection.
In this embodiment, the rubber buffer 5 is cylindrical, and with the shape adaptation of extrusion chamber 108, the bottom of rubber buffer 5 is equipped with the opening, and inside cavity, top are the pressing contact surface.
Referring to fig. 3, the nucleic acid is generally eluted in the elution chamber 109 at last, so that the target object is present in the mixed solution in the elution chamber 109, at this time, since the number of through holes through which the reaction tube 7 communicates with the elution chamber 109 is generally small, and the speed of flowing into the reaction tube 7 is slow, the rubber stopper 5 needs to be pressed through the bottom of the magnetic sleeve 3, so that the air inside the rubber stopper 5 is pressed into the reaction tube 7 through the through holes at the bottom of the pressing chamber 108, and then discharged from the elution chamber 109 through the through holes through which the reaction tube 7 communicates with the elution chamber 109, and then the magnetic sleeve 3 is taken out, at this time, due to the elastic force return of the rubber stopper 5, the hollow space inside forms an adsorption effect on the reaction tube 7, so that the pressure inside the reaction tube 7 is reduced, and further, the solution inside the elution chamber 109 can flow into the reaction tube 7 rapidly under the action of atmospheric pressure for reaction.
In this embodiment, the tube cover 4 is cylindrical and fits the shape of the elution chamber 109, and the tube cover 4 is a plastic cover. In the process of making the mixed liquid in the elution chamber 109 flow into the reaction tube 7 quickly by pressing the rubber plug 5, after taking out the magnetic sleeve 3, the detection instrument takes out the tube cap 4 in the tube cap chamber 107, and moves the tube cap 4 into the elution chamber 109 to plug and seal the elution chamber 109, thereby avoiding the influence from the outside in the detection process.
In this embodiment, the reagent tube 6 is fixed at one side in the conversion chamber 105, and is a separately packaged tube body, and the bottom of the reagent tube 6 is a thin film structure easy to pierce. When the conversion is performed, the magnetic sleeve 3 is inserted into the reagent tube 6, and the thin film structure at the bottom of the reagent tube 6 can be easily punctured, so that the conversion combined liquid flow in the reagent tube 6 falls into the conversion cavity 105 to be mixed with the conversion liquid, and the conversion of the nucleic acid is completed.
In this embodiment, the magnetic sleeve 3 is a hollow cylindrical tube, and has a closed conical bottom end and an open top end. The magnetic rod of the detection instrument can be inserted into the magnetic sleeve 3 through the opening, so that the magnetic sleeve 3 can adsorb the magnetic beads 2 in the reagent with magnetism. At the same time, the conical shape of the bottom end of the magnetic sleeve 3 can be conveniently used to pierce some membranes.
In this embodiment, the cross section of the cavity is one of a square, a polygon and a circle, and may be other irregular shapes, which may be formulated as required.
In this embodiment, the sealing film is a film made of a plastic film, an aluminum film, or the like, and is sealed by heat sealing or ultrasonic welding. The sealing film is in close contact with the top of the reagent strip 1 to play a role of sealing the cavity, so that the reagent in the cavity is limited in the cavity, and the magnetic sleeve 3 can easily pierce the sealing film.
In this embodiment, the bottom or/and the outer wall of the lysis chamber 101 and the conversion chamber 105 are externally connected to a heating module of the detection instrument, and the heating module is used for heating the chamber body to further accelerate the sample lysis and conversion.
The detailed description is given to the detection process of the cervical cancer gene methylation detection device:
the device comprises a lysis chamber 101, a conversion chamber 105, a desulfonation chamber 106, four washing chambers 104 from left to right, washing solutions with high concentration, moderate concentration and low concentration are sequentially pre-filled in the lysis chamber 101, the four washing chambers 104 are sequentially pre-filled with washing solutions with high concentration, moderate concentration and low concentration, an eluent is pre-filled in an elution chamber 109, a conversion binding solution is pre-filled in a reagent tube 6, and a freeze-dried PCR reaction solution is pre-filled in a reaction tube 7;
thirteen cavities of the reagent strip 1 are sequentially named as a first cavity, a second cavity, a.once.a.thirteen cavity from left to right, namely, the first cavity is a cracking cavity 101, the second and fourth cavities are magnetic bead cavities 102, the third cavity is a magnetic sleeve cavity 103, the sixth cavity is a conversion cavity 105, the seventh cavity is a desulfonation cavity 106, the fifth, eighth, ninth and tenth cavities are washing cavities 104, the eleventh cavity is a tube cover cavity 107, the twelfth cavity is an extrusion cavity 108, and the thirteenth cavity is an elution cavity 109;
the specific detection process comprises the following steps:
(a) Sample adding: opening a sealing film on the reagent strip 1, adding a detection sample into the first cavity, and then putting the whole cervical cancer gene methylation detection device into a detection instrument according to a positioning structure;
(b) Sample extraction and methylation conversion:
1) Sample lysis: the detection instrument moves the magnetic sleeve 3 from the third cavity into the first cavity, and then the magnetic sleeve 3 moves up and down or rotates in the first cavity to uniformly mix and crack the detection sample in the first cavity; meanwhile, the bottom of the first cavity is heated by a detection instrument, so that the sample cracking is further accelerated;
2) Nucleic acid adsorption: the detection instrument moves the magnetic sleeve 3 into the second cavity, so that the magnetic sleeve 3 moves up and down or rotates in the cavity, and the magnetic beads 2 in the cavity are uniformly mixed; a magnetic rod on the detection instrument is downwards inserted into the magnetic sleeve 3 to adsorb the magnetic beads 2 in the cavity, and the magnetic beads 2 are adsorbed on the magnetic sleeve 3; the detection instrument moves the magnetic sleeve 3 and the magnetic rod together into a first cavity, withdraws the magnetic rod, releases the magnetic beads 2 adsorbed on the magnetic sleeve 3, and the magnetic sleeve 3 moves up and down in the cavity to uniformly mix the reagent and the magnetic beads 2 in the cavity, so that the nucleic acid in the detection sample is adsorbed on the magnetic beads 2;
3) Nucleic acid transformation: a magnetic rod of the detection instrument is downwards inserted into a magnetic sleeve 3, and the magnetic sleeve 3 adsorbs the magnetic beads 2 in the first cavity; the magnetic sleeve 3 and the magnetic rod move together into the No. six cavity, the magnetic rod is removed, the magnetic beads 2 adsorbed on the magnetic sleeve 3 are released, the magnetic sleeve 3 moves up and down in the No. six cavity to uniformly mix the conversion solution and the magnetic beads 2 in the cavity, and meanwhile, a detection instrument starts a conversion program;
4) Nucleic acid binding: the detection instrument moves the magnetic sleeve 3 to the position above the reagent tube 6 in the cavity of No. six, then the magnetic sleeve 3 moves downwards all the time, the bottom end of the magnetic sleeve 3 pierces the bottom of the reagent tube 6, so that the conversion binding reagent in the reagent tube 6 flows into the cavity of No. six below to be mixed with the conversion solution, then the detection instrument moves the magnetic sleeve 3 to the cavity of No. four, so that the magnetic sleeve 3 moves up and down or rotates in the cavity, and the uniform mixing of the magnetic beads 2 in the cavity is realized; a magnetic rod on the detection instrument is downwards inserted into the magnetic sleeve 3 to adsorb the magnetic beads 2 in the cavity, and the magnetic beads 2 are adsorbed on the magnetic sleeve 3; the detection instrument moves the magnetic sleeve 3 and the magnetic rod together into a No. six cavity, withdraws the magnetic rod, releases the magnetic beads 2 adsorbed on the magnetic sleeve 3, and the magnetic sleeve 3 moves up and down in the cavity to uniformly mix the solution in the cavity and the magnetic beads 2, so that the target substance in the detection sample is adsorbed on the magnetic beads 2;
5) And (3) desulfurating: a magnetic rod of the detection instrument is downwards inserted into a magnetic sleeve 3, and the magnetic sleeve 3 adsorbs magnetic beads 2 in the cavity III; the magnetic sleeve 3 and the magnetic rod move together into the fifth cavity, the magnetic rod is removed, the magnetic beads 2 adsorbed on the magnetic sleeve 3 are released, the magnetic sleeve 3 moves up and down in the fifth cavity to uniformly mix the reagent and the magnetic beads 2 in the cavity, and the magnetic beads 2 are preliminarily washed; then transferring the magnetic beads 2 to a No. seven cavity by the same method, and carrying out desulfonation through a desulfonation liquid;
6) Washing: a magnetic rod of the detection instrument is downwards inserted into a magnetic sleeve 3, and the magnetic sleeve 3 adsorbs the magnetic beads 2 in the No. seven cavity; the magnetic sleeve 3 and the magnetic rod move together into the eighth cavity, the magnetic rod is removed, the magnetic beads 2 adsorbed on the magnetic sleeve 3 are released, and the magnetic sleeve 3 moves up and down in the eighth cavity to uniformly mix and clean the reagent and the magnetic beads 2 in the cavity; then transferring the magnetic beads 2 to a No. nine cavity by the same method for secondary cleaning; then transferring the magnetic beads 2 to a No. ten cavity by the same method for three times of cleaning;
7) Nucleic acid elution: the detection instrument moves the magnetic sleeve 3 and the magnetic rod together into a cavity of No. thirteen, withdraws the magnetic rod, releases the magnetic beads 2 adsorbed on the magnetic sleeve 3, and the magnetic sleeve 3 moves up and down in the cavity of No. thirteen to uniformly mix the eluent and the magnetic beads 2 in the cavity;
8) Discarding magnetic beads: a magnetic rod of the detection instrument is downwards inserted into the magnetic sleeve 3 to adsorb the magnetic beads 2 in the cavity of No. thirteen, and the magnetic beads 2 are adsorbed on the magnetic sleeve 3; the detection instrument moves the magnetic sleeve 3 and the magnetic rod together into the No. ten cavity, the magnetic rod is removed, the magnetic beads 2 adsorbed on the magnetic sleeve 3 are discarded in the No. ten cavity, the nucleic acid extraction and methylation conversion are completed, and the cleaned nucleic acid is mixed in the mixed solution of the No. thirteen cavity;
(c) And (3) PCR detection: the detection instrument moves the magnetic sleeve 3 into the No. twelve cavity, then moves the magnetic sleeve 3 downwards all the time, so that the bottom end of the magnetic sleeve 3 presses the rubber plug 5 in the No. twelve cavity downwards, then moves the magnetic sleeve 3 upwards, so that the rubber plug 5 returns to the original position due to the self elasticity, at the moment, the pressure in the reaction tube 7 can be reduced due to the internal resetting of the rubber plug 5 for air suction, so that the reagent containing the target in the No. thirteen cavity can flow into the reaction tube 7 below at an accelerated speed, meanwhile, the detection instrument moves the tube cover 4 in the No. eleven cavity into the No. thirteen cavity, the tube cover 4 is utilized to block and seal the No. thirteen cavity, finally, the reagent containing the nucleic acid is mixed with the detection reagent in the reaction tube 7, and the detection instrument starts a PCR program for detection;
(d) The device discards: and after the PCR detection is finished, taking the cervical cancer sample detection device out of the detector for waste biological treatment.
The utility model discloses with cervical cancer sample schizolysis, nucleic acid conversion, nucleic acid purification, fluorescence PCR detection integration in an integral type detection device for cervical cancer gene methylation detects can full-automatic completion, need not manual sample pretreatment, has reduced operating error, has reduced the human cost. The utility model discloses a detection device has greatly simplified overall structure, and disposable can avoid the cross contamination between the sample to improve the sample and draw and the accuracy that detects, only need one step of application of sample operation, convenient to use further is applied to clinically providing wide prospect for cervical carcinoma gene methylation detection technology.
Example 2:
as shown in FIG. 2, this embodiment is similar to embodiment 1, except that in this embodiment, the bottom of the elution chamber 109 is inclined, and the lowest end is provided with a through hole, which is communicated with the reaction tube 7. The inclined bottom can accelerate the mixed liquid to flow into the reaction tube 7 through the through hole at the bottom of the elution cavity 109, and a vortex effect may be formed when the pressure in the reaction tube 7 is reduced under the inclined action, so as to accelerate the inflow.
Example 3:
as shown in FIG. 2, this embodiment is similar to embodiment 1 except that in this embodiment, the conversion chamber 105 is provided with a slope at a position corresponding to the bottom of the reagent vessel 6. When the transformation is carried out, the magnetic sleeve 3 is extended into the reagent tube 6, so that the thin film structure at the bottom of the reagent tube 6 can be easily punctured, the transformation binding solution in the reagent tube 6 can rapidly flow down through the inclined surface and is mixed with the transformation solution in the transformation cavity 105, the transformation of nucleic acid is completed, and the transformation efficiency is improved.
It is obvious that the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not limitations to the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. This need not be, nor should it be exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. The cervical cancer gene methylation detection device is characterized by comprising a reagent strip (1), wherein the reagent strip (1) is provided with at least nine cavities which are not communicated with each other, and the top of each cavity is provided with a sealing structure for sealing all the cavities; the cavity body comprises a lysis cavity (101) for lysing a sample, a magnetic bead cavity (102) pre-filled with magnetic beads (2), a magnetic sleeve cavity (103) pre-filled with a magnetic sleeve (3), a conversion cavity (105) for conversion, a desulfonation cavity (106) for desulfonation, a washing cavity (104) for washing, an extrusion cavity (108) pre-filled with a rubber plug (5), a tube cover cavity (107) pre-filled with a tube cover (4), and an elution cavity (109) for elution;
a reagent tube (6) is arranged in the conversion cavity (105), and conversion binding liquid is pre-filled in the reagent tube (6);
a reaction tube (7) is fixed at the bottom of the reagent strip (1), the reaction tube (7) is communicated with through holes at the bottoms of the elution cavity (109) and the extrusion cavity (108), and PCR reaction liquid is pre-filled in the reaction tube (7);
except the magnetic bead cavity (102), the magnetic sleeve cavity (103), the extrusion cavity (108) and the tube cover cavity (107), other cavities are respectively preloaded with different reagents for sample processing.
2. The cervical cancer gene methylation detection device according to claim 1, wherein the rubber stopper (5) is cylindrical and is matched with the extrusion chamber (108) in shape, the rubber stopper (5) is provided with an opening at the bottom, is hollow inside and has a pressing contact surface at the top.
3. The cervical cancer gene methylation detection apparatus according to claim 1, wherein the tube cap (4) is cylindrical and is adapted to the shape of the elution cavity (109), and the tube cap (4) is a plastic cap.
4. The cervical cancer gene methylation detection device according to claim 1, wherein the bottom of the elution cavity (109) is inclined, and the lowest end of the elution cavity is provided with a through hole which is communicated with the reaction tube (7).
5. The cervical cancer gene methylation detection device according to claim 1, wherein the reagent tube (6) is fixed at one side in the transformation cavity (105), the transformation cavity (105) is provided with an inclined surface at the corresponding position of the bottom of the reagent tube (6), and the bottom of the reagent tube (6) is of a thin film structure which is easy to pierce.
6. The cervical cancer gene methylation detection device according to claim 1, wherein four washing chambers (104) are provided, and the four washing chambers (104) are respectively pre-filled with washing liquids with different concentrations.
7. The cervical cancer gene methylation detection device according to claim 1, wherein the magnetic sleeve (3) is hollow cylindrical tube-shaped, the bottom end of the magnetic sleeve is in a closed conical shape, and the top end of the magnetic sleeve is open.
8. The apparatus for detecting methylation of a cervical cancer gene according to claim 1, wherein the sealing structure is a sealing film, and the sealing film is sealed by heat sealing or ultrasonic welding.
9. The cervical cancer gene methylation detection apparatus according to claim 1, wherein the bottom or/and the outer wall of the lysis chamber (101) and the transformation chamber (105) is externally connected to a heating module of a detection instrument.
10. The cervical cancer gene methylation detection device according to claim 1, wherein the bottom of the reaction tube (7) is externally connected to a detection instrument, and the detection instrument is used for performing PCR reaction and detection on the reaction tube (7).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202222357112.5U CN218507779U (en) | 2022-09-05 | 2022-09-05 | Cervical cancer gene methylation detection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202222357112.5U CN218507779U (en) | 2022-09-05 | 2022-09-05 | Cervical cancer gene methylation detection device |
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| CN218507779U true CN218507779U (en) | 2023-02-21 |
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| CN202222357112.5U Active CN218507779U (en) | 2022-09-05 | 2022-09-05 | Cervical cancer gene methylation detection device |
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