CN106472091A - Corn seed purity field plot field plot test method - Google Patents
Corn seed purity field plot field plot test method Download PDFInfo
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- CN106472091A CN106472091A CN201610906502.XA CN201610906502A CN106472091A CN 106472091 A CN106472091 A CN 106472091A CN 201610906502 A CN201610906502 A CN 201610906502A CN 106472091 A CN106472091 A CN 106472091A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The present invention relates to corn seed authentication method, especially corn seed purity field plot field plot test method, comprise the following steps：(1) to for the registration of sample product, sequence；(2) obtain standard sample and collect the description of variety characteristic；(3) determine the grain number of plantation sample；(4) number takes plantation sample and numbers；(5) select plot；Choose two kinds of cultivation modes, respectively Ridged plant peace furrow wide-narrow row planting according to rainfall distributed areas respectively, described Ridged plant adopts in area of heavy rainfull, and described flat furrow wide-narrow row planting adopts in area short of rain；(6) sow；(7) seedling stage assay；(8) florescence identification；(9) period of maturation identification.The method, in the periods such as Maize Seedlings, florescence, period of maturation, the difference of plant forms feature and biological characteristicses, identifies special-shaped plant, thus identifying seed purity it is ensured that seed quality is it is ensured that safety is planted in agricultural production.
Description
Technical field
The present invention relates to corn seed authentication method, especially corn seed purity field plot field plot test method.
Background technology
Improved seeds productive potentialities are largely dependent upon seed quality, and the verity of seed and purity are to evaluate to plant
The good and bad important indicator of protonatomic mass, the lower general who has surrendered of purity leads to the obvious reduction of seed production and quality.Field trapping test method
As the most accurate, Purity method the most authoritative, therefore there is seed purity in people one straight grip field plot test for a long time
During dispute, typically all arbitration inspection is carried out with field plot test.During field plot test, the selection to plantation soil is more single at present
One, generally in June early and middle ten dayses, now milpa growth is in especially florescence megathermal period on the occasion of high temperature rain to seedtime
In season, it is unfavorable for the abundant performance of corn variety feature；Thickness of sowing is generally lower than normal plantation, for identification kind
Plant, be unfavorable for the growth of Semen Maydiss, affect qualification result.Generally adopt florescence identification, identification number of times is less, and result is had an impact.
Content of the invention
For solving the above problems, the present invention provides a kind of single cave sowing, the sowing of different soils, one kind of multiple contrast record
Corn seed purity field plot field plot test method, concrete technical scheme is：
Corn seed purity field plot field plot test method, comprises the following steps：
(1) to for the registration of sample product, sequence；
According to variety name, source, working year, seed quality index, registered and by kind sequence, according to this row
Sequence number writes specimen coding；
(2) obtain standard sample and collect the description of variety characteristic；
The standard sample of parent and cenospecies is collected at breeder and its agent or variety managements department, and collection
Plant feature related data, including sheath color, leaf margin color, leaf, plant shape, plant height, Ear height, filigree color, tassel and branch
How much, anther color, fringe shape, cob color, particle shape, grain color；
(3) determine the grain number of plantation sample；
According to state quality standard《GB 4404.1-2008》The Reinheitszahl of regulation or label for labelling Reinheitszahl, to determine examination
Test the plantation strain number of design, if standard is (N-1) × 100%/N, plantation strain number is 4N, because each sample germination percentage differs, is
Ensure that plantation strain number meets 4N principle, plantation sample grain number should be 4N/ germination percentage；
(4) number takes plantation sample and numbers；
Plant the grain number of sample according to step (3), random number takes two parts of field plot test sample from for sample product, according to confession
Specimen coding is write in the sequence number of sample product, is easy to the sample adjacent seed same kind；
(5) select plot；
Each sample sets 2 repetitions, and the sample adjacent seed of same kind, then adjacent seed standard sample is cenospecies
Or Parent does comparison and is compared, to judge its typical case, consistent degree, often repeat to plant two row, using north and south row to plantation；
Choose two kinds of cultivation modes, respectively Ridged plant peace furrow wide-and narrow-row kind according to rainfall distributed areas respectively
Plant, described Ridged plant adopts in area of heavy rainfull, described flat furrow wide-narrow row planting adopts in area short of rain；
(6) sow；
Adopt simple grain bunch planting in sowing time, use bamboo stem labelling, long bamboo stem presses fixing spacing in the rows amount good position, uses mark
Labelling is as the site of program request；Use plastic ties labelling, plastic ties press fixing spacing in the rows amount good position, uses different colours line
Rope binding marking, as the site of program request, with bamboo stem and plastic ties as scale, makes spacing in the rows uniformity, and plant erects
Straight it is ensured that the growing space of the equalization of every plant；
(7) seedling stage assay；With seedling sheath color, leaf, leaf color, leaf edge color, blade ripple and degreeof tortuosity and length
Gesture is identified, and fills in corn seed purity field plot field plot test application form；
(8) florescence identification；Had or not with fine hair on plant type, leaf, leaf color, leaf, vein color, auricle shape, tassel shape
And branch loose compactness, sterile lemma color, flower pesticide color, filigree color, blade opening angle, the number of blade and fringe position how many with branch,
Panel length is identified, and fills in corn seed purity field plot field plot test application form；
(9) period of maturation identification；Entered with reference to resistance, period of duration with fringe type, particle shape, grain color, seed size and cob color
Row identification, and fill in corn seed purity field plot field plot test application form.
Described Ridged plant, row spacing 100-130cm, furrow width 30cm about, ditch depth 20-30cm；Using duplicate rows kind on ridge
Plant, the line-spacing of its duplicate rows is 100cm, and as observation road, Planting Row Distance ridge side 10-15cm on ridge, according to sample size and plant
Away from determining cell length and width, cell surrounding sets 4-5 row protection row；Described flat furrow wide-narrow row planting, wide row line-spacing 80-
90cm, as observation road；Narrow row 30-40cm；Spacing in the rows 30-40cm.
Described sowing time was broadcast for evening, and 30-July 10 Ji ALFISOL IN CENTRAL June is sowed, and planted the sowing time than maize field
Postpone 20 days.
It is chosen at area of heavy rainfull Ridged plant according to rainfall distributed areas respectively and in regional flat furrow wide-and narrow-row kind short of rain
Plant, reduce the impact to cultivar identification for the rainfall, that is, reduce the impact to Purity Identification for the extraneous factor.
Using simple grain bunch planting, and mark in detail, be easy to record and manage, ensure the growth of the equalization of every plant simultaneously
Space, reduces the error that external factor leads to.
Normal field corn this area June sowing time early and middle ten dayses, it is especially colored that milpa growth is in the megathermal period
Phase, on the occasion of high temperature rainy season, is unfavorable for the abundant performance of corn variety feature, Postpone sowing time, can improve its growth ring
Border, beneficial to florescence identification.
Increase the identification in seedling stage and period of maturation.Corn variety quantity is very many at present, and between kind on good multiple characters
Have points of resemblance, increase the identification in seedling stage and period of maturation, using three periods feature synthetic determination it is ensured that qualification result
Accurate.
Random number takes test specimen.For make limited seed have preferably representative it is necessary to random by regulation seed amount
Perfect number takes seed to be sowed.Because if the seed grain number that number takes is more than the Seeding Quantity of regulation, during program request
Can choose whichever is to one personal advantage, be left the seed of slight of stature, cause personal error.
The corn seed purity field plot field plot test method that the present invention provides is in Maize Seedlings, florescence, period of maturation
, according to the difference of plant forms feature and biological characteristicses, different in nature plant is identified in period, thus identifying seed purity,
Guarantee seed quality it is ensured that safety is planted in agricultural production.
Specific embodiment
Corn seed purity field plot field plot test method, comprises the following steps：
(1) to for the registration of sample product, sequence；
According to variety name, source, working year, seed quality index, registered and by kind sequence, according to this row
Sequence number writes specimen coding.
(2) obtain standard sample and collect the description of variety characteristic；
The standard sample of parent and cenospecies is collected at breeder and its agent or variety managements department, and collection
Plant feature related data, including sheath color, leaf margin color, leaf, plant shape, plant height, Ear height, filigree color, tassel and branch
How much, anther color, fringe shape, cob color, particle shape, grain color.
(3) determine the grain number of plantation sample；
According to state quality standard《GB 4404.1-2008》The Reinheitszahl of regulation or label for labelling Reinheitszahl, to determine examination
Test the plantation strain number of design.If standard is (N-1) × 100%/N, plantation strain number is 4N, because each sample germination percentage differs, is
Ensure that plantation strain number meets 4N principle, plantation sample grain number should be 4N/ germination percentage.
(4) number takes plantation sample and numbers；
Plant the grain number of sample according to step (3), random number takes two parts of field plot test sample from for sample product, according to confession
Specimen coding is write in the sequence number of sample product, is easy to the sample adjacent seed same kind.
(5) select plot；
Select the suitable plant identification traitses normal development of climatic environment condition, soil fertility uniformly, fertile soil, have irrigation and drainage bar
Part, front stubble are not Semen Maydiss and the plot of no disease and pests harm and weeds.
Each sample sets 2 repetitions, and the sample adjacent seed of same kind, then adjacent seed standard sample is cenospecies
Or Parent does comparison and is compared, to judge its typical case, consistent degree, often repeat to plant two row, using north and south row to plantation；
Choose two kinds of cultivation modes, respectively Ridged plant peace furrow wide-and narrow-row kind according to rainfall distributed areas respectively
Plant, described Ridged plant adopts in area of heavy rainfull, row spacing 100-130cm, furrow width 30cm about, ditch depth 20-30cm；Using ridge
Upper duplicate rows plantation, the line-spacing of its duplicate rows is 100cm, and as observation road, Planting Row Distance ridge side 10-15cm on ridge, according to sample number
Amount and seeding row spacing determine cell length and width, and cell surrounding sets 4-5 row protection row；
Described flat furrow wide-narrow row planting adopts in area short of rain, wide row line-spacing 80-90cm, as observation road, narrow row 30-
40cm；Spacing in the rows 30-40cm.
(6) sow；
Pre-sowing water should be poured before broadcasting, fine site preparation, use sufficient base fertilizer, N, P, K should join in proportion and apply.
Seed should be carried out before broadcasting with dry in the sun, promote germination.For preventing insect pest and the plague of rats, should carry out to being uncoated seed
Dressing seed etc. is processed.
Adopt simple grain bunch planting in sowing time, use bamboo stem labelling, long bamboo stem presses fixing spacing in the rows amount good position, uses mark
Labelling is as the site of program request；Use plastic ties labelling, plastic ties press fixing spacing in the rows amount good position, uses different colours line
Rope binding marking, as the site of program request, with bamboo stem and plastic ties as scale, makes spacing in the rows uniformity, and plant erects
Directly it is ensured that the growing space of the equalization of every plant, depth of planting 3-5cm depth of planting, thickness of earth covering and pressing dynamics will be use up
Amount is consistent.
Sowing time was broadcast for evening, and 30-July 10 Ji ALFISOL IN CENTRAL June is sowed, and postponed than the maize field plantation sowing time
20 days.
The time of infertility at least intertills twice, especially after raining, loosening the soil in time it is ensured that having gone out Seedling, must not fill the gaps with seedlings.
Carry out in seedling stage 5-6 leaf for the first time, tilling depth 3-4cm, second carries out deep cultivation and banking inferior to after jointing before envelope ridge.Also can be
Spray herbicide after finishing.
Topdress once in the jointing stage, watering for advocating peace to combine with quick-acting N fertilizer is carried out.To pour permeable before taking out hero, prevention
Card neck drought, pours a water to increase grain plumpness before grouting.
The harm of the disease such as insect pest and smut, virosiss such as preventing and treating subterranean pest-insect, mythimna separata, Pyrausta nubilalis (Hubern). and aphid in time.
(7) seedling stage assay；With seedling sheath color, leaf, leaf color, leaf edge color, blade ripple and degreeof tortuosity and length
Gesture is identified, and fills in corn seed purity field plot field plot test application form, such as table 1；
(8) florescence identification；Had or not with fine hair on plant type, leaf, leaf color, leaf, vein color, auricle shape, tassel shape
And branch loose compactness, sterile lemma color, flower pesticide color, filigree color, blade opening angle, the number of blade and fringe position how many with branch,
Panel length is identified, and fills in corn seed purity field plot field plot test application form；
(9) period of maturation identification；Entered with reference to resistance, period of duration with fringe type, particle shape, grain color, seed size and cob color
Row identification, and fill in corn seed purity field plot field plot test application form.
Identification must be completed jointly by more than two accredited Seed Inspection persons.With father, female parent and the cenospecies mark collected
The phene of quasi- sample is as standard.Fill in identification strictly according to the facts to record, strain label or plastics board are made to be suspected to seedling stage, florescence
Labelling.Synthetic determination again after the identification of each period of duration, still thinks that the plant being like right and wrong should be by this breed count.
Computing formula：
Use《GB/T3543.5》The Deviation of Eggs Per Gram of table 2 regulation, if can not find out in table, available following formula is calculated：
P variety mark value；
Q=100-P；
N kind plants strain number.
Qualification result with seedling stage as reference, with tree characteristics performance the most completely, based on the most obvious florescence and fruit ear phase, with
The meansigma methodss repeating twice represent, retain a decimal.Figure correction executes《GB8170-87 Figure correction rule》.Various one-tenth
The percentage rate summation divided is necessary for 100.0%.
Table 1 corn seed purity field plot field plot test application form
Explanation：Write the color of anthocyanin when the 1st, describing exactly, and indicate weak, medium, strong；
2nd, in addition to the content of this table regulation, other contents with kind typical case's character all can be supplemented.
Claims (3)
1. field plot test method in corn seed purity field plot is it is characterised in that comprise the following steps：
(1) to for the registration of sample product, sequence；
According to variety name, source, working year, seed quality index, registered and by kind sequence, according to this sequence number
Write specimen coding；
(2) obtain standard sample and collect the description of variety characteristic；
Collect the standard sample of parent and cenospecies at breeder and its agent or variety managements department, and it is special to collect kind
Intrinsic characteristics related data, including sheath color, leaf margin color, leaf, plant shape, plant height, Ear height, filigree color, tassel and branch how many,
Anther color, fringe shape, cob color, particle shape, grain color；
(3) determine the grain number of plantation sample；
According to state quality standard《GB 4404.1-2008》The Reinheitszahl of regulation or label for labelling Reinheitszahl, to determine that test sets
The plantation strain number of meter, if standard is (N-1) × 100%/N, plantation strain number is 4N, because each sample germination percentage differs, for ensureing
Plantation strain number meets 4N principle, and plantation sample grain number should be 4N/ germination percentage；
(4) number takes plantation sample and numbers；
Plant the grain number of sample according to step (3), random number takes two parts of field plot test sample from for sample product, foundation supplies sample
Specimen coding is write in the sequence number of product, is easy to the sample adjacent seed same kind；
(5) select plot；
Each sample sets 2 repetitions, and the sample adjacent seed of same kind, then adjacent seed standard sample is cenospecies or father
Female parent does comparison and is compared, and to judge its typical case, consistent degree, often repeats to plant two row, using north and south row to plantation；
Choose two kinds of cultivation modes, respectively Ridged plant peace furrow wide-narrow row planting, institute according to rainfall distributed areas respectively
State Ridged plant to adopt in area of heavy rainfull, described flat furrow wide-narrow row planting adopts in area short of rain；
(6) sow；
Adopt simple grain bunch planting in sowing time, use bamboo stem labelling, long bamboo stem presses fixing spacing in the rows amount good position, uses marking pen mark
It is denoted as the site for program request；Use plastic ties labelling, fixing spacing in the rows amount good position is pressed on plastic ties, with different colours cotton rope bundle
Tie up marking, as the site of program request, with bamboo stem and plastic ties as scale, make spacing in the rows uniformity, and plant is vertically, protects
The growing space of the equalization of every plant of card；
(7) seedling stage assay；Entered with seedling sheath color, leaf, leaf color, leaf edge color, blade ripple and degreeof tortuosity and growing way
Row identification, and fill in corn seed purity field plot field plot test application form；
(8) florescence identification；Had or not with fine hair on plant type, leaf, leaf color, leaf, vein color, auricle shape, tassel shape and point
Branch is how many and the loose compactness of branch, sterile lemma color, flower pesticide color, filigree color, blade opening angle, the number of blade and fringe position, internode
Length is identified, and fills in corn seed purity field plot field plot test application form；
(9) period of maturation identification；Reflected with reference to resistance, period of duration with fringe type, particle shape, grain color, seed size and cob color
Fixed, and fill in corn seed purity field plot field plot test application form.
2. corn seed purity field plot according to claim 1 field plot test method is it is characterised in that described ridging
Plantation, row spacing 100-130cm, furrow width 30cm about, ditch depth 20-30cm；Planted using duplicate rows on ridge, the line-spacing of its duplicate rows is
100cm, as observation road, Planting Row Distance ridge side 10-15cm on ridge, according to sample size and seeding row spacing determine cell length and
Width, cell surrounding sets 4-5 row protection row；Described flat furrow wide-narrow row planting, wide row line-spacing 80-90cm, as observation road；Narrow
Row 30-40cm；Spacing in the rows 30-40cm.
3. corn seed purity field plot according to claim 1 field plot test method is it is characterised in that described sowing
Phase was broadcast for evening, and 30-July 10 Ji ALFISOL IN CENTRAL June is sowed, and postponed 20 days than the maize field plantation sowing time.
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Cited By (4)
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| CN108650946A (en) * | 2018-05-08 | 2018-10-16 | 兰州大学 | A kind of uniform type of seeding of grid location based on crop ecology experiment |
| CN110651626A (en) * | 2018-09-10 | 2020-01-07 | 湖南省林产品质量检验检测中心 | Identification method of masson pine and black pine seeds |
| CN111837865A (en) * | 2020-06-28 | 2020-10-30 | 中国农业科学院棉花研究所 | Method for identifying purity of cotton variety and operation steps thereof |
| CN112931099A (en) * | 2021-03-23 | 2021-06-11 | 石家庄市种子管理站 | Method for identifying purity of winter wheat seeds by planting in field plot |
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| CN111837865A (en) * | 2020-06-28 | 2020-10-30 | 中国农业科学院棉花研究所 | Method for identifying purity of cotton variety and operation steps thereof |
| CN112931099A (en) * | 2021-03-23 | 2021-06-11 | 石家庄市种子管理站 | Method for identifying purity of winter wheat seeds by planting in field plot |
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Application publication date: 20170308 |