CN108283114A - A kind of molecular breeding method of height cold resistance corn seed - Google Patents
A kind of molecular breeding method of height cold resistance corn seed Download PDFInfo
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- CN108283114A CN108283114A CN201810067915.2A CN201810067915A CN108283114A CN 108283114 A CN108283114 A CN 108283114A CN 201810067915 A CN201810067915 A CN 201810067915A CN 108283114 A CN108283114 A CN 108283114A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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Abstract
The invention discloses a kind of molecular breeding methods of height cold resistance corn seed, are related to corn breeding techniques field.Include the following steps；Selection of land：The field of selected shape rule, and soil is divided into two parts；Prepartion of land；Apply base manure；Sowing；It is managed before bud；Plant manages；Secondary sowing；New varieties obtain；Seed screens；Gene alteration；Cold resistance seed obtains.Method through the invention improves the germination success rate in breeding process；Female processing is gone by carrying out emasculation respectively simultaneously, and by going female plant to carry out artificial pollination, improving the breeding speed and success rate of breeding of corn variety.
Description
Technical field
The invention belongs to corn breeding techniques fields, more particularly to a kind of molecular breeding of height cold resistance corn seed
Method.
Background technology
Corn is the maximum cereal crops of China's cultivated area, and production capacity and China's grain security are closely related.Training
It is the important channel realized grain and constantly increased production to educate stable high yield eurytopicity corn variety.Corn variety cultivation period is long, mistake
Journey is complicated, and yielding ability, yield stability and the Regional suitability of precise Identification corn variety are the important rings during corn variety is cultivated
Section.
For the yielding stability for ensureing improved variety and extensive adaptability, breeder needs in part for many years, more places
New varieties (combination) are evaluated.Large-scale multiple spot test is mostly used to the evaluation of corn variety both at home and abroad at present, is set
The Combined Stress etc. for setting adverse circumstance such as high density, low fertilizer, arid or the more adverse circumstances of setting carries out screening and assessment, and key is to it
Resistance is identified and is evaluated.Different planting densities, which are arranged, to carry out the single plant production capacity of kind and comprehensive resistance
Effective evaluation, but be easy to select and type kind is depended on unduly to density.Multiple years test can actively be sought corn growth season
The various adverse environmental factors being likely encountered, as a result accurately and reliably, but the ecological environment item due to different ecological region, not between the same year border
Part is changeful, and test data differs greatly, and is affected to qualification result, is brought very to the selection and appraisal of breeder
Big difficult and deviation.And these identification methods expend a large amount of human and material resources, financial resources, breeding cost is excessively high, non-general breeding units
It can bear.One kind is fitted on from material group into production, needs the time of more than ten years, the breed breeding period is long, main cultivation product
Kind update is slow, it is difficult to meet the growth requirement in production and market.
Invention content
The purpose of the present invention is to provide a kind of molecular breeding methods of height cold resistance corn seed, through the invention
Method improves the germination success rate in breeding process；Female processing is gone by carrying out emasculation respectively simultaneously, and is passed through
To going female plant to carry out artificial pollination, the breeding speed and success rate of breeding of corn variety are improved.
In order to solve the above technical problems, the present invention is achieved by the following technical solutions：
The present invention is a kind of molecular breeding method of height cold resistance corn seed, is included the following steps：
SS01, selection of land：Select floor space between the field of 0.5-3 mus of regular shape, and will be native using a divider wall
Ground is divided into two parts；
SS02, prepartion of land：1) weeding is carried out to soil with herbicidal spray after soil drying；2) leaching of pouring water is carried out to soil
Bubble pours water and is higher than soil 1-2cm to water level；3) it using the deep soil loosing shovel chiselling with subsoiling bill, deep ploughs as 40-60cm；4)
Rotary plow carries out hack to soil, while being paved soil using the pull rod at rotary plow rear；
SS03, apply base manure：1) apply plant ash to field, rape shell folder covering plant ash is used in combination；2) with mouldboard plow pair
Field digged, hack and covering；3) apply farm manure to field, and pour water and impregnated to water level higher than soil 3-5cm；
4) it is dried after impregnating, covers the black mud that a layer thickness is 1-2cm to soil surface, continue to dry in black mud；
SS04, sowing：1) select suitable for local plantation, Resistant, that tiller is weak, anti-fall, fringe is big, setting percentage is high is high-quality,
High-yield variety is as corn female parent；2) it is sowed on field using seeder, after planting sprays a small amount of nitrogenous fertilizer solution；3) it broadcasts
It is sprayed water to field by sprayer after kind；
It is managed before SS05, bud：It sheds broken rice husk or broken beanpod and cladding thickness is carried out between 0.5-2cm to field；Night makes
Covering treatment is carried out to soil with film, covering film is opened daytime, until germination；
SS06, plant management：Respectively spraying fertilising is carried out in sprouting stage, jointing stage, florescence, development of grain phase；
Wherein, emasculation processing is carried out to the corn on a portion field；To the corn on wherein another part field
Carry out female processing；
SS07, seed collection：The corn seed handled through emasculation is collected as F1 generation female parent, collects the jade through going female processing
Rice seed is as F1 generation male parent；
SS07, secondary sowing：F1 generation female parent and F1 generation male parent are sowed respectively, harvest the pollen of F1 generation paternal plant to F1
The plant of godmother's sheet carries out artificial pollination；
SS08, new varieties obtain：It plants experimentally, screen by multiple spot, obtain corn variety；
SS09, seed screening：Two parts are filtered out with monosome segment the substitution line S1 and S2 for stablizing cold-resistant QTL；Mark
Remember the cold-resistant QTLqCTS-6 close linkages that PSM1 and PSM2 is carried with monosome segment substitution line S1, marks RM17 and PSM3
The cold-resistant QTLqCTS-12 close linkages carried with monosome segment substitution line S2；
SS10, gene alteration：By screening obtain with stablize the monosome segment substitution line S1 and S2 of cold-resistant QTL into
Row hybridization, using cold-resistant QTLqCTS-6 and qCTS-12 close linkages label to polymerization system F1, F2, F3 for group carry out with
Track screens the strain for having target gene type, and obtaining homozygous cold-resistant QTL in F2 or F3 generations polymerize system；
SS11, cold resistance seed obtain：The polymerization system of acquisition is carried out to the cold resistance evaluation of target growth developmental stage, sieve
Choosing obtains the excellent rice strain that cold resistance significantly improves.
Further, the divider wall used in the SS01 selection of lands is the isolation that a height for using soil to erect is 50cm
Ridge, wherein the sowing spacing of the adjacent both sides in the isolation ridge is 2-3m.
Further, herbicide uses acetamide-group herbicides, institute in the herbicidal spray used in the SS02 prepartions of land
It includes Acetochlor or alachlor or butachlor or isopropyl methoxalamine or propisochlor to state acetamide-group herbicides；The use of the herbicide
Amount is 60-80g/ mus.
Further, the SS03 applies in base manure, and the dosage of the plant ash is 20-50kg/ mus；The rape shell folder
Dosage be 200-500kg/ mus；The dosage of the farm manure is 400-800kg/ mus.
Further, spray in the SS06 plant management fertilising nitrogen potassium phosphorus total content between 20-60g/L.
The invention has the advantages that：
The method of the present invention through the invention improves the germination success rate in breeding process；Pass through difference simultaneously
It carries out emasculation and then goes female processing, and by going female plant to carry out artificial pollination, improving the breeding speed of corn variety
And success rate of breeding；The molecular breeding method of the present invention is by molecular marker assisted selection, from traditional resistance to the specific period
Cold property phenotypic evaluation and selection are improved to the selection to genotype, and the selection of genotype can not be given birth in early stage into row is said
The limitation of phase, therefore, this cold resistance breeding new method improve the efficiency and accuracy of breeding.
Certainly, it implements any of the products of the present invention and does not necessarily require achieving all the advantages described above at the same time.
Specific implementation mode
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
All other embodiment that art personnel are obtained without creative efforts belongs to the model that the present invention protects
It encloses.
The present invention is a kind of molecular breeding method of height cold resistance corn seed, is included the following steps：
SS01, selection of land：Select floor space between the field of 0.5-3 mus of regular shape, and will be native using a divider wall
Ground is divided into two parts；
SS02, prepartion of land：1) weeding is carried out to soil with herbicidal spray after soil drying；2) leaching of pouring water is carried out to soil
Bubble pours water and is higher than soil 1-2cm to water level；3) it using the deep soil loosing shovel chiselling with subsoiling bill, deep ploughs as 40-60cm；4)
Rotary plow carries out hack to soil, while being paved soil using the pull rod at rotary plow rear；
SS03, apply base manure：1) apply plant ash to field, rape shell folder covering plant ash is used in combination；2) with mouldboard plow pair
Field digged, hack and covering；3) apply farm manure to field, and pour water and impregnated to water level higher than soil 3-5cm；
4) it is dried after impregnating, covers the black mud that a layer thickness is 1-2cm to soil surface, continue to dry in black mud；
SS04, sowing：1) select suitable for local plantation, Resistant, that tiller is weak, anti-fall, fringe is big, setting percentage is high is high-quality,
High-yield variety is as corn female parent；2) it is sowed on field using seeder, after planting sprays a small amount of nitrogenous fertilizer solution；3) it broadcasts
It is sprayed water to field by sprayer after kind；
It is managed before SS05, bud：It sheds broken rice husk or broken beanpod and cladding thickness is carried out between 0.5-2cm to field；Night makes
Covering treatment is carried out to soil with film, covering film is opened daytime, until germination；
SS06, plant management：Respectively spraying fertilising is carried out in sprouting stage, jointing stage, florescence, development of grain phase；
Wherein, emasculation processing is carried out to the corn on a portion field；To the corn on wherein another part field
Carry out female processing；
SS07, seed collection：The corn seed handled through emasculation is collected as F1 generation female parent, collects the jade through going female processing
Rice seed is as F1 generation male parent；
SS07, secondary sowing：F1 generation female parent and F1 generation male parent are sowed respectively, harvest the pollen of F1 generation paternal plant to F1
The plant of godmother's sheet carries out artificial pollination；
SS08, new varieties obtain：It plants experimentally, screen by multiple spot, obtain corn variety；
SS09, seed screening：Two parts are filtered out with monosome segment the substitution line S1 and S2 for stablizing cold-resistant QTL；Mark
Remember the cold-resistant QTLqCTS-6 close linkages that PSM1 and PSM2 is carried with monosome segment substitution line S1, marks RM17 and PSM3
The cold-resistant QTLqCTS-12 close linkages carried with monosome segment substitution line S2；
SS10, gene alteration：By screening obtain with stablize the monosome segment substitution line S1 and S2 of cold-resistant QTL into
Row hybridization, using cold-resistant QTLqCTS-6 and qCTS-12 close linkages label to polymerization system F1, F2, F3 for group carry out with
Track screens the strain for having target gene type, and obtaining homozygous cold-resistant QTL in F2 or F3 generations polymerize system；
SS11, cold resistance seed obtain：The polymerization system of acquisition is carried out to the cold resistance evaluation of target growth developmental stage, sieve
Choosing obtains the excellent rice strain that cold resistance significantly improves.
Preferably, the divider wall used in SS01 selection of lands is the isolation ridge that a height for using soil to erect is 50cm,
The middle sowing spacing that the adjacent both sides in ridge are isolated is 2-3m.
Preferably, herbicide uses acetamide-group herbicides, amides to remove in the herbicidal spray used in SS02 prepartions of land
Careless agent includes Acetochlor or alachlor or butachlor or isopropyl methoxalamine or propisochlor；The dosage of herbicide is 60-80g/ mus.
Preferably, SS03 applies in base manure, and the dosage of plant ash is 20-50kg/ mus；The dosage of rape shell folder is 200-
500kg/ mus；The dosage of farm manure is 400-800kg/ mus.
Preferably, SS06 plant management in spray fertilising nitrogen potassium phosphorus total content between 20-60g/L.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one implementation of the present invention
In example or example.In the present specification, schematic expression of the above terms may not refer to the same embodiment or example.
Moreover, particular features, structures, materials, or characteristics described can be in any one or more of the embodiments or examples to close
Suitable mode combines.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to preferably explain the present invention
Principle and practical application, to enable skilled artisan to be best understood by and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (4)
1. a kind of molecular breeding method of height cold resistance corn seed, which is characterized in that include the following steps：
SS01, selection of land：It selects floor space between the field of 0.5-3 mus of regular shape, and is divided soil using a divider wall
For two parts；
SS02, prepartion of land：1) weeding is carried out to soil with herbicidal spray after soil drying；2) immersion of pouring water is carried out to soil,
It pours water and is higher than soil 1-2cm to water level；3) it using the deep soil loosing shovel chiselling with subsoiling bill, deep ploughs as 40-60cm；4) rotary tillage
Plough carries out hack to soil, while being paved soil using the pull rod at rotary plow rear；
SS03, apply base manure：1) apply plant ash to field, rape shell folder covering plant ash is used in combination；2) with mouldboard plow to field
It is digged, hack and covering；3) apply farm manure to field, and pour water and impregnated to water level higher than soil 3-5cm；4) it soaks
It is dried after bubble, covers the black mud that a layer thickness is 1-2cm to soil surface, continue to dry in black mud；
SS04, sowing：1) it selects suitable for local plantation, Resistant, tiller is weak, anti-fall, fringe is big, setting percentage is high high-quality, high yield
Kind is as corn female parent；2) it is sowed on field using seeder, after planting sprays a small amount of nitrogenous fertilizer solution；3) after planting
It is sprayed water to field by sprayer；
It is managed before SS05, bud：It sheds broken rice husk or broken beanpod and cladding thickness is carried out between 0.5-2cm to field；Night uses thin
Film carries out covering treatment to soil, covering film is opened daytime, until germination；
SS06, plant management：Respectively spraying fertilising is carried out in sprouting stage, jointing stage, florescence, development of grain phase；
Wherein, emasculation processing is carried out to the corn on a portion field；Corn on wherein another part field is carried out
Go female processing；
SS07, seed collection：The corn seed handled through emasculation is collected as F1 generation female parent, collects the maize seed through going female processing
Son is used as F1 generation male parent；
SS07, secondary sowing：F1 generation female parent and F1 generation male parent are sowed respectively, harvest the pollen of F1 generation paternal plant to F1 generation mother
This plant carries out artificial pollination；
SS08, new varieties obtain：It plants experimentally, screen by multiple spot, obtain corn variety；
SS09, seed screening：Two parts are filtered out with monosome segment the substitution line S1 and S2 for stablizing cold-resistant QTL；Label
The cold-resistant QTLqCTS-6 close linkages that PSM1 and PSM2 and monosome segment substitution line S1 is carried, label RM17 and PSM3 with
The cold-resistant QTLqCTS-12 close linkages that monosome segment substitution line S2 is carried；
SS10, gene alteration：Screening is obtained miscellaneous with monosome segment substitution line S1 and the S2 progress for stablizing cold-resistant QTL
It hands over, F1, F2, F3 of polymerization system is sieved for group into line trace using the label of cold-resistant QTLqCTS-6 and qCTS-12 close linkages
Choosing has the strain of target gene type, and obtaining homozygous cold-resistant QTL in F2 or F3 generations polymerize system；
SS11, cold resistance seed obtain：The cold resistance evaluation that the polymerization system of acquisition is carried out to target growth developmental stage, is screened
The excellent rice strain significantly improved to cold resistance.
2. a kind of molecular breeding method of height cold resistance corn seed according to claim 1, which is characterized in that described
It includes second grass that herbicide, which uses acetamide-group herbicides, the acetamide-group herbicides, in the herbicidal spray used in SS02 prepartions of land
Amine or alachlor or butachlor or isopropyl methoxalamine or propisochlor；The dosage of the herbicide is 60-80g/ mus.
3. a kind of molecular breeding method of height cold resistance corn seed according to claim 1, which is characterized in that described
SS03 applies in base manure, and the dosage of the plant ash is 20-50kg/ mus；The dosage of the rape shell folder is 200-500kg/ mus；
The dosage of the farm manure is 400-800kg/ mus.
4. a kind of molecular breeding method of height cold resistance corn seed according to claim 1, which is characterized in that described
SS06 plant management in spray fertilising nitrogen potassium phosphorus total content between 20-60g/L.
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| CN201810067915.2A CN108283114A (en) | 2018-01-24 | 2018-01-24 | A kind of molecular breeding method of height cold resistance corn seed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114097603A (en) * | 2021-11-28 | 2022-03-01 | 西安农链互联网科技有限公司 | Castration quality-keeping method for hybrid corn seed production |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1769463A (en) * | 2005-09-22 | 2006-05-10 | 山东大学 | Method for promoting salt and drought tolerance of maize and wheat by combining betA,NHX1,PPase gene and transgene technology |
| CN102960241A (en) * | 2012-12-11 | 2013-03-13 | 云南西都种业有限责任公司 | Seed selection and breeding method of hybrid corn new species |
| CN105475120A (en) * | 2015-12-11 | 2016-04-13 | 广东省农业科学院水稻研究所 | Molecular breeding method for improving cold resistance of rice |
| CN106560023A (en) * | 2016-09-16 | 2017-04-12 | 漯河市农业科学院 | Wheat breeding method |
| CN106613919A (en) * | 2017-01-18 | 2017-05-10 | 纪骏炜 | Hybrid corn seeds production method in low-temperature environment |
-
2018
- 2018-01-24 CN CN201810067915.2A patent/CN108283114A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1769463A (en) * | 2005-09-22 | 2006-05-10 | 山东大学 | Method for promoting salt and drought tolerance of maize and wheat by combining betA,NHX1,PPase gene and transgene technology |
| CN102960241A (en) * | 2012-12-11 | 2013-03-13 | 云南西都种业有限责任公司 | Seed selection and breeding method of hybrid corn new species |
| CN105475120A (en) * | 2015-12-11 | 2016-04-13 | 广东省农业科学院水稻研究所 | Molecular breeding method for improving cold resistance of rice |
| CN106560023A (en) * | 2016-09-16 | 2017-04-12 | 漯河市农业科学院 | Wheat breeding method |
| CN106613919A (en) * | 2017-01-18 | 2017-05-10 | 纪骏炜 | Hybrid corn seeds production method in low-temperature environment |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114097603A (en) * | 2021-11-28 | 2022-03-01 | 西安农链互联网科技有限公司 | Castration quality-keeping method for hybrid corn seed production |
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