CN103960522A - Pichia pastoris liquid feed for crabs and preparation method of pichia pastoris liquid feed - Google Patents

Abstract

The invention discloses a pichia pastoris liquid feed for crabs and a preparation method of the pichia pastoris liquid feed. The pichia pastoris liquid feed is characterized by being prepared from the following raw materials in parts by weight: 8-12 parts of brewer's grain, 7-9 parts of pichia pastoris liquid, 1-2 parts of sodium tripolyphosphate, 14-17 parts of sea fish, 21-28 parts of cottonseed meal, 16-18 parts of maize, 1-2 parts of rice vinegar, 4-6 parts of safflower oil, 14-18 parts of celery, 16-19 parts of sweet wormwood leaf, 20-25 parts of carrot, 0.4-0.6 part of berberis julianae schneid, 1-2 parts of endothelium corneum gigeriae galli, 3-5 parts of flos chrysanthemi indici, 3-6 parts of a phagostimulant, and a defined amount of water. The pichia pastoris liquid feed provided by the invention has the advantages of high utilization ratio, low morbidity, high growth rate and capability of purifying a water body.

Description

A kind of Pichia yeast liquid crab feed and preparation method thereof

Technical field

The present invention relates to field of fodder, be specifically related to a kind of Pichia yeast liquid crab feed and preparation method thereof.

Background technology

The food variety of crab is various, small fish and shrimp is their favorite, but some crab eats marine alga, connecting even spoil or plant can eat, they spend with the most of the time at search of food, not particular about food, so long as the food that can chew can be eaten, this has exactly also affected the fresh and tender and taste of crab meat.In addition fresh or freezing small fish and the fish meal of the many employings of the animal fodder of a lot of crabs is in the market primary raw material, there is shortcomings such as destroying the stock of fish and easy polluted source, these feeds easily become pasty state in water, do not meet the eating habit that crab is liked molded food product; The shortcomings such as in addition, existing crab feed kind is single, and trophic structure is uneven, easy putrid and deteriorated.So find that a kind of trophic structure is balanced, palatability is good, not facile hydrolysis the feed that accommodates crab each growth period of the novel green health of polluted source is not extremely urgent.

Summary of the invention

Object of the present invention is just to provide a kind of Pichia yeast liquid crab feed and preparation method thereof, to overcome the deficiencies in the prior art.

The object of the present invention is achieved like this:

A kind of Pichia yeast liquid crab feed, it is characterized in that, by the raw material of following weight portion, made: brewex's grains 8-12, Pichia yeast liquid 7-9, sodium phosphate trimer 1-2, ocean fish 14-17, cotton dregs 21-28, corn 16-18, rice vinegar 1-2, safflower seed oil 4-6, celery 14-18, artemisia leaf 16-19, carrot 20-25, barberry 0.4-0.6, the membrane of a chicken's gizzard 1-2, mother chrysanthemum 3-5, phagostimulant 3-6, suitable quantity of water.

Described phagostimulant is made by following raw materials in part by weight: marine alga 14-17, Enteromorpha 16-23, soya-bean cake 20-25, cuttlefish internal organ 17-19, ethoxyquinoline 0.3-0.6, shell element 0.2-0.4, glucose 3-5, sodium Diacetate 0.5-0.8, vermiculite 14-18, biotite 15-19, sorbic acid 1-3, carbonic hydroammonium 2-4, citric acid 1.2-3.5, mountain crow pearl leaf 5-9, caryophyllus oil 3-6, inosinicacid 2.1-4.3, suitable quantity of water, and its preparation method is by cuttlefish internal organ, caryophyllus oil together fried dry and grinds to form seafood powder; Marine alga, Enteromorpha are added to suitable quantity of water and put into together cooking machine and break into dish juice, add soya-bean cake to boil 30-40 minute, smash into soya-bean cake mud to pieces; Vermiculite, biotite are put into calcining furnace and at 514-570 ℃, calcine 2-3 hour, take out, the hydrochloric acid solution of putting into 10-13% soaks 4-5 hour, filter and take out, use clear water cyclic washing, dry and pulverize, be added to the water, meanwhile, add sorbic acid, carbonic hydroammonium, citric acid, mountain crow pearl leaf to grind, the dry xeraphium that makes of spraying; Finally the seafood powder making, soya-bean cake mud, xeraphium and other residual components are added to water and mix, grind granulation and get final product.

The preparation method of described a kind of Pichia yeast liquid crab feed, is characterized in that comprising the following steps:

(1) celery, artemisia leaf are added to suitable quantity of water and put into cooking machine and break into dish juice, carrot is cut into slices and in boiling water, scalded, put into dish juice and soak 3-6 hour, pull out to dry and pulverize;

(2) barberry, the membrane of a chicken's gizzard, mother chrysanthemum are added to water and in water, decoct together 1-2 hour and filter, get its filter residue fried dry to grind to form mixed powder standby together with cotton dregs, corn;

(3) add ocean fish, rice vinegar to stir-fry kardiseed rusting heat, add the middle drug filtrate of step (2), Pichia yeast liquid to boil together 40-50min, pull oven dry out, pulverize standby;

(4) step (1), (2), (3) reaction mass and other residual components are added to water and stir, through 90-110 ℃ of steaming 40 minutes, cooling, granulation, balling-up particle diameter, at 0.5-2mm, is dried and be get final product.

All chemical classes additive components such as ethoxyquinoline, shell element, glucose, sodium Diacetate, sorbic acid, inosinicacid in the present invention, all adopt dosage form to add, and do not adopt sterling form to add, and addition should meet national Specification, does not exceed standard.

The present invention has following beneficial effect: Pichia yeast liquid crab feed of the present invention has the advantages such as utilization rate is high, the incidence of disease is low, fast growth, purifying water body.

The specific embodiment

Described a kind of Pichia yeast liquid crab feed, it is characterized in that, by the raw material of following weight portion, made: brewex's grains 8, Pichia yeast liquid 7, sodium phosphate trimer 1, ocean fish 17, cotton dregs 21, corn 18, rice vinegar 1, safflower seed oil 4, celery 14, artemisia leaf 19, carrot 20, barberry 0.4, the membrane of a chicken's gizzard 1, mother chrysanthemum 3, phagostimulant 6, suitable quantity of water.

Described phagostimulant is made by following raw materials in part by weight: marine alga 16, Enteromorpha 19, soya-bean cake 20, cuttlefish internal organ 17, ethoxyquinoline 0.3, shell element 0.24, glucose 3, sodium Diacetate 0.58, vermiculite 14, biotite 15, sorbic acid 1, carbonic hydroammonium 2, citric acid 1.2, mountain crow pearl leaf 9, caryophyllus oil 3, inosinicacid 2.1, suitable quantity of water, and its preparation method is by cuttlefish internal organ, caryophyllus oil together fried dry and grinds to form seafood powder; Marine alga, Enteromorpha are added to suitable quantity of water and put into together cooking machine and break into dish juice, add soya-bean cake to boil 30-40 minute, smash into soya-bean cake mud to pieces; Vermiculite, biotite are put into calcining furnace and at 514-570 ℃, calcine 2-3 hour, take out, the hydrochloric acid solution of putting into 10-13% soaks 4-5 hour, filter and take out, use clear water cyclic washing, dry and pulverize, be added to the water, meanwhile, add sorbic acid, carbonic hydroammonium, citric acid, mountain crow pearl leaf to grind, the dry xeraphium that makes of spraying; Finally the seafood powder making, soya-bean cake mud, xeraphium and other residual components are added to water and mix, grind granulation and get final product.

Preparation method comprises the following steps:

(1) celery, artemisia leaf are added to suitable quantity of water and put into cooking machine and break into dish juice, carrot is cut into slices and in boiling water, scalded, put into dish juice and soak 3-6 hour, pull out to dry and pulverize;

(2) barberry, the membrane of a chicken's gizzard, mother chrysanthemum are added to water and in water, decoct together 1-2 hour and filter, get its filter residue fried dry to grind to form mixed powder standby together with cotton dregs, corn;

(3) add ocean fish, rice vinegar to stir-fry kardiseed rusting heat, add the middle drug filtrate of step (2), Pichia yeast liquid to boil together 40-50min, pull oven dry out, pulverize standby;

(4) step (1), (2), (3) reaction mass and other residual components are added to water and stir, through 90-110 ℃ of steaming 40 minutes, cooling, granulation, balling-up particle diameter, at 0.5-2mm, is dried and be get final product.

In order to further illustrate using value of the present invention, raise family 200 crabs are equally divided into two groups of test group and control groups according to the identical principle of age in days, body weight, health status by crab, separately feed, test group is fed crab feed of the present invention, control group is fed traditional crab feed, experimental period is 90 days, compares with control group, and the every technical parameter index of test group is as follows:

(1) the long balanced growth 10.78% of body;

(2) sick rate reduces by 14.12%;

(3) water float thing is only 0.07%;

(4) meat consolidation, shell is firmly crisp, and shell color and luster green grass or young crops is bright.

Claims (2)

1. a Pichia yeast liquid crab feed, it is characterized in that, by the raw material of following weight portion, made: brewex's grains 8-12, Pichia yeast liquid 7-9, sodium phosphate trimer 1-2, ocean fish 14-17, cotton dregs 21-28, corn 16-18, rice vinegar 1-2, safflower seed oil 4-6, celery 14-18, artemisia leaf 16-19, carrot 20-25, barberry 0.4-0.6, the membrane of a chicken's gizzard 1-2, mother chrysanthemum 3-5, phagostimulant 3-6, suitable quantity of water; Described phagostimulant is made by following raw materials in part by weight: marine alga 14-17, Enteromorpha 16-23, soya-bean cake 20-25, cuttlefish internal organ 17-19, ethoxyquinoline 0.3-0.6, shell element 0.2-0.4, glucose 3-5, sodium Diacetate 0.5-0.8, vermiculite 14-18, biotite 15-19, sorbic acid 1-3, carbonic hydroammonium 2-4, citric acid 1.2-3.5, mountain crow pearl leaf 5-9, caryophyllus oil 3-6, inosinicacid 2.1-4.3, suitable quantity of water, and its preparation method is by cuttlefish internal organ, caryophyllus oil together fried dry and grinds to form seafood powder; Marine alga, Enteromorpha are added to suitable quantity of water and put into together cooking machine and break into dish juice, add soya-bean cake to boil 30-40 minute, smash into soya-bean cake mud to pieces; Vermiculite, biotite are put into calcining furnace and at 514-570 ℃, calcine 2-3 hour, take out, the hydrochloric acid solution of putting into 10-13% soaks 4-5 hour, filter and take out, use clear water cyclic washing, dry and pulverize, be added to the water, meanwhile, add sorbic acid, carbonic hydroammonium, citric acid, mountain crow pearl leaf to grind, the dry xeraphium that makes of spraying; Finally the seafood powder making, soya-bean cake mud, xeraphium and other residual components are added to water and mix, grind granulation and get final product.

2. the preparation method of a kind of Pichia yeast liquid crab feed according to claim 1, is characterized in that comprising the following steps:

(1) celery, artemisia leaf are added to suitable quantity of water and put into cooking machine and break into dish juice, carrot is cut into slices and in boiling water, scalded, put into dish juice and soak 3-6 hour, pull out to dry and pulverize;

(2) barberry, the membrane of a chicken's gizzard, mother chrysanthemum are added to water and in water, decoct together 1-2 hour and filter, get its filter residue fried dry to grind to form mixed powder standby together with cotton dregs, corn;

(3) add ocean fish, rice vinegar to stir-fry kardiseed rusting heat, add the middle drug filtrate of step (2), Pichia yeast liquid to boil together 40-50min, pull oven dry out, pulverize standby;

(4) step (1), (2), (3) reaction mass and other residual components are added to water and stir, through 90-110 ℃ of steaming 40 minutes, cooling, granulation, balling-up particle diameter, at 0.5-2mm, is dried and be get final product.