CN103404729A - Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics - Google Patents
Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics Download PDFInfo
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Abstract
The invention provides a composite microecologics capable of improving growth rate of stichopus japonicus and application of the composite microecologics. The composite microecologics comprises bacillus subtilis, saccharomyces cerevisiae, cellulase, pectinase, xylanase, neutral protease, solid fermentation lactic acid bacteria and a carrier, wherein the bacterium content of the bacillus subtilis is greater than or equal to 1* 108 per g, the bacterium content of the saccharomyces cerevisiae is greater than or equal to 1* 109 per g, the content of the cellulase is greater than or equal to 1,000 U/g, the content of the pectinase is greater than or equal to 1,200 U/g, the content of the xylanase is greater than or equal to 1,500 U/g, the content of the neutral protease is greater than or equal to 12,000 U/g, the bacterium content of the solid fermentation lactic acid bacteria is greater than or equal to 200 mg/g, and the content of the carrier is greater than or equal to 500 mg/g. The composite microecologics can effectively improve the utilization efficiency of bait by stichopus japonicus and promote the growth of stichopus japonicus through adding zymin; the added fungicide can restrain reproduction of pathogenic bacteria in a water body and reduce the occurrence probability of diseases such as skin melting, head shaking, swollen mouth and viscus discharging; the composite microecologics has the advantages of safety, efficiency, specifity, no residue, no side effect and the like.
Description
Technical field
The invention belongs to the livestock feed technical field, relate to feed addictive, be specifically related to a kind of compound micro-ecological preparation and application thereof that improves stichopus japonicus growth rate.
Background technology
On apostichopus japonicus culture, great majority are to utilize the feed of the collocation certain proportion oozes such as algae powder, scallop powder as stichopus japonicus at present, and the ratio of ooze is between 20%-50%.Except ooze, in feedstuff, the vegetable raw material such as algae powder is occupied an leading position, and therefore contains more SNSP, and animal digestion is absorbed and has certain anti-oxidant action.Stichopus japonicus is from the young to become ginseng alginase vigor to be in reduced levels always, show stichopus japonicus itself to sea-tangle and undaria pinnitafida etc. be rich in alignic large-scale algae digestion power a little less than.The food of stichopus japonicus forms and has determined in its feed that algin and cellulose occupy significant proportion, but stichopus japonicus itself secretes the limited in one's ability of alginase and cellulase, and feed conversion rate is low, and growth cycle is long.Growth and breeding to stichopus japonicus is unfavorable.
Summary of the invention
For apostichopus japonicus Selenka feed vegetable raw material composition, reach the low situation of stichopus japonicus enteron aisle enzyme activity more, the invention provides a kind of compound micro-ecological preparation and application thereof that improves stichopus japonicus growth rate, deficiency with commercial enzyme preparation and the supplementary stichopus japonicus enteron aisle digestive ferment of bacteria preparation, and occupy-place and the bacteria resistance function of strengthening beneficial bacterium, reduce stichopus japonicus intestines problem occurrence probability, and promoted the growth of stichopus japonicus.
For achieving the above object, the present invention adopts following technical proposals to be achieved:
A kind of compound micro-ecological preparation that improves stichopus japonicus growth rate, described compound micro-ecological preparation comprises following component: the bacterial content of bacillus subtilis is not less than 1 * 10
8Individual/g, the bacterial content of S. cervisiae is not less than 1 * 10
9Individual/g, cellulase content is not less than 1000U/g, and pectase content is not less than 1200U/g, zytase content is not less than 1500U/g, neutral proteinase content is not less than 12000U/g, and the bacterial content of solid fermentation lactic acid bacteria is not less than 200mg/g, and vector contg is not less than 500mg/g.
Further, described probiotics contains: the bacterial content of bacillus subtilis is 1 * 10
8Individual/g; The bacterial content of S. cervisiae is 1 * 10
9Individual/g; Cellulase content is 1000U/g; Pectase content is 1200U/g; Zytase content is 1500U/g; Neutral proteinase content is 12000U/g; The bacterial content of solid fermentation lactic acid bacteria is 200mg/g, and vector contg is 500mg/g.
Further, described carrier is for adding beneficial powder, and 60 order oversizes are 0.
The present invention also provides the application of described compound micro-ecological preparation in the feed of preparation raising stichopus japonicus growth rate, and the addition of described compound micro-ecological preparation is according to 3 ‰ of perfect compound feed gross weight-5 ‰.
Further, directly described compound micro-ecological preparation is added in stichopus japonicus daily ration perfect compound feed.
Further, described compound micro-ecological preparation and feed addictive or feedstuff are mixed and made into to premix or concentrate feed form, add seawater to make pasty state, the 12-24h that ferments in advance makes the pasty state fermentation material, feeds to stichopus japonicus after then the pasty state fermentation material being mixed in the ratio of 1:2-3 with bright ooze.
Compared with prior art, advantage of the present invention and technique effect are: the present invention has designed a kind of novel compound micro-ecological preparation according to stichopus japonicus growth characteristics and feedstuff composition, the concrete complex enzymes such as commercial neutral proteinase, cellulase, zytase and pectase of selecting carry out enzymolysis, can supplement the deficiency of digestive ferment in the stichopus japonicus enteron aisle, in order to improve the digestibility of vegetable protein, starch, effectively promote the stichopus japonicus growth.
The selected business-like bacillus subtilis strain low temperature germination rate of the present invention is high, the S. cervisiae reproduction speed is fast, after with four kinds of enzyme preparations such as cellulase, pectase, zytase, neutral proteinases, coordinating feed being fermented in advance, can reduce ANFs in vegetable raw material, promote the decomposition of high molecular weight protein and carbohydrate, improve the digestibility of stichopus japonicus, in conjunction with the solid fermentation lactic acid bacteria, its contained lactic acid bacteria metabolite can effectively suppress the breeding of the pathogens such as vibrios, gas pseudomonad, build healthy intestinal environment, promote growth.
When concrete application, in advance feed is carried out to pre-fermentation and also made up the low impact that enzyme preparation is played a role of breeding water body temperature, bacillus subtilis and saccharomycetic breeding can be stichopus japonicus high-quality single cell protein bletilla biology antibacterial substance are provided in this process simultaneously, have reduced the occurrence probability of intestines problem.
To sum up, compound micro-ecological preparation of the present invention can effectively improve the utilization ratio of stichopus japonicus to bait by adding enzyme preparation, promote the growth of stichopus japonicus, add the breeding that microbial inoculum can suppress the pathogenic bacteria such as Aeromonas hydrophila in water body, molten algae blood vibrios, reductionizations skin, the mouth of shaking the head, swell, arrange the occurrence probability of the disease such as dirty; With similar additive, compare have security, high efficiency, selectivity, noresidue and the advantage such as have no side effect; Use described compound micro-ecological preparation can improve and maintain the stichopus japonicus growth conditions, effectively improve its growth rate.
The accompanying drawing explanation
Fig. 1 is that interpolation time and the children of compound micro-ecological preparation of the present invention joins the lab diagram of specification to the enzyme preparation effect.
The specific embodiment
The present invention is further detailed explanation in conjunction with following specific embodiment in the present invention.
Embodiment 1
The compound micro-ecological preparation of raising stichopus japonicus growth rate of the present invention comprises following component: the bacterial content of bacillus subtilis is not less than 1 * 10
8Individual/g, the bacterial content of S. cervisiae is not less than 1 * 10
9Individual/g, cellulase content is not less than 1000U/g, and pectase content is not less than 1200U/g, zytase content is not less than 1500U/g, neutral proteinase content is not less than 12000U/g, and the bacterial content of solid fermentation lactic acid bacteria is no less than 200mg/g, and vector contg is not less than 500mg/g.
The compound micro-ecological preparation of the described raising of the present embodiment stichopus japonicus growth rate comprises: bacillus subtilis 1 * 10
8Individual/g, S. cervisiae 1 * 10
9Individual/g, cellulase 1000U/g, pectase 1200U/g, zytase 1500U/g, neutral proteinase 12000U/g, solid fermentation lactic acid bacteria 200mg/g, carrier 500mg/g.The described carrier of the present embodiment is for adding beneficial powder, and 60 order oversizes are 0.
The application of compound micro-ecological preparation of the present invention in the feed of preparation raising stichopus japonicus growth rate: the addition of described compound micro-ecological preparation is 3 ‰ of perfect compound feed gross weight-5 ‰, directly described compound micro-ecological preparation is added in stichopus japonicus daily ration perfect compound feed; Or be mixed and made into premix, concentrate feed form with other feed addictive or feedstuff, and add appropriate seawater, make pasty state, pre-fermentation 24h makes the pasty state fermentate, then after the pasty state fermentate being mixed in the ratio of 1:2-3 with bright ooze, feed to stichopus japonicus, to improve stichopus japonicus growth rate.
Embodiment 2, compound micro-ecological preparation of the present invention are tested the impact of stichopus japonicus growth
Test aquarium volume is 45L, uninterruptedly inflation during cultivation.Test is divided into 4 groups, and the 1st group is added the 5g/kg enzyme preparation, and the 2nd group is added microbial inoculum 10
9CFU/kg, the 3rd group is added enzyme preparation 5g/kg+ microbial inoculum 10
9CFU/kg, the 4th group is control group, does not add enzyme preparation.Each organizes 6 repetitions, and an aquarium is a repetition, and each aquarium is put 8 stichopus japonicus in a suitable place to breed, and specification is 9.8g ± 0.2/, and the test period is 40d.
The 1st group of enzyme preparation of adding is in compound micro-ecological preparation, to remove the composition of microbial inoculum, namely the cellulase that contains described content, pectase, zytase, neutral proteinase, consists of; The 2nd group of microbial inoculum added is in compound micro-ecological preparation, to remove the composition of enzyme preparation, namely the bacillus subtilis that contains described content, S. cervisiae and solid fermentation lactic acid bacteria, consists of; The 3rd group is added enzyme preparation+microbial inoculum is compound micro-ecological preparation of the present invention.
As can be seen from Table 1, the compound microecological group of throwing something and feeding growth rate is significantly higher than control group (P<0.05), the enzyme preparation of throwing something and feeding separately or microbial inoculum, the growth rate of stichopus japonicus on average improves 8.33% than control group respectively, 9.57%(P > 0.05), explanation is by cellulase, pectase, zytase, the enzyme preparation that neutral proteinase forms and by bacillus subtilis, S. cervisiae and solid fermentation lactic acid bacteria composition bacteria preparation have facilitation to a certain degree to the growth of stichopus japonicus, after enzyme preparation and bacteria preparation are composite, stichopus japonicus is increased to effect and be better than independent participation (P > 0.05).
Embodiment 3, compound micro-ecological preparation of the present invention are joined the impact of growth on the children
Select between 2 stichopus japonicus seedling culturing vehicles to carry out enzyme preparation and add test.Each nursery pond volume is 20 side's water, and seedling pond water temperature is between 11-13 ℃.The compound micro-ecological preparation addition is the 5g/kg feed, and the blank group is set.Test period is divided into 20d and 25d, 20d group only select 4 order specifications as subjects, the 25d group selects respectively 4 orders and two kinds of specifications of 6 orders to test, for relatively adding the impact on the enzyme preparation effect of time and children's ginseng specification.Each test arranges 6 repetitions, the weight of the every pond of weighing children's ginseng when on-test and end.
Experimental result as shown in Figure 1.No matter be 4 order seedlings or 6 order seedlings, after in bait, continuing to add the compound micro-ecological preparation of 5g/kg, the average growth rate of the 20-25d of the young all significantly improves (P ﹤ 0.05).20d4 order, 25d4 order, 25d6 order rate of body weight gain are respectively than control group and improve 14.05%, 23.27% and 26.79%, comparatively speaking, along with compound micro-ecological preparation, add the prolongation of time in feed, children's ginseng rate of body weight gain improves more obvious, but under identical feeding time, the test of 4 orders and two kinds of specifications of 6 orders children ginseng rate of body weight gain there was no significant difference.Before starting just the experience temperature of cultivation reduce process, in pond, seedling has that part skin, swollen mouth, corn are not endured, enteron aisle is not good, epidermis is not thick, drags and just wait phenomenon.In 20-25d off-test while falling the pond,, most of seedling state takes a turn for the better, and the seedling look deepens green, and very, ight soil is shaped, does not drag just the corn point, and the end is not smelly, swollen mouth, changes the skin seedling that is not in good state and obviously reduces.
Above embodiment is only in order to technical scheme of the present invention to be described, but not is limited; Although with reference to previous embodiment, the present invention is had been described in detail, for the person of ordinary skill of the art, still can modify to the technical scheme that previous embodiment is put down in writing, or part technical characterictic wherein is equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution break away from the spirit and scope of the present invention's technical scheme required for protection.
Claims (6)
1. compound micro-ecological preparation that improves stichopus japonicus growth rate, it is characterized in that: described compound micro-ecological preparation comprises following component: the bacterial content of bacillus subtilis is not less than 1 * 108/g, the bacterial content of S. cervisiae is not less than 1 * 109/g, cellulase content is not less than 1000 U/g, pectase content is not less than 1200 U/g, zytase content is not less than 1500 U/g, neutral proteinase content is not less than 12000 U/g, the bacterial content of solid fermentation lactic acid bacteria is not less than 200 mg/g, and vector contg is not less than 500 mg/g.
2. a kind of compound micro-ecological preparation that improves stichopus japonicus growth rate according to claim 1, it is characterized in that: described probiotics comprises: the bacterial content of bacillus subtilis is 1 * 108/g; The bacterial content of S. cervisiae is 1 * 109/g; Cellulase content is 1000 U/g; Pectase content is 1200 U/g; Zytase content is 1500 U/g; Neutral proteinase content is 12000 U/g; The bacterial content of solid fermentation lactic acid bacteria is 200 mg/g, and vector contg is 500 mg/g.
3. a kind of compound micro-ecological preparation that improves stichopus japonicus growth rate according to claim 1, it is characterized in that: described carrier is for adding beneficial powder, and 60 order oversizes are 0.
4. the application in the feed of preparation raising stichopus japonicus growth rate according to claim 1 or 2 or 3 described compound micro-ecological preparations, it is characterized in that: the addition of described compound micro-ecological preparation is according to 3 ‰ of perfect compound feed gross weight-5 ‰.
5. the application of compound micro-ecological preparation according to claim 4 in the feed of preparation raising stichopus japonicus growth rate, is characterized in that: directly described compound micro-ecological preparation is added in stichopus japonicus daily ration perfect compound feed.
6. the application of compound micro-ecological preparation according to claim 4 in the feed of preparation raising stichopus japonicus growth rate, it is characterized in that: described compound micro-ecological preparation and feed addictive or feedstuff are mixed and made into to premix or concentrate feed form, add seawater to make pasty state, pre-fermentation 12-24h makes the pasty state fermentation material, feeds to stichopus japonicus after then the pasty state fermentation material being mixed in the ratio of 1:2-3 with bright ooze.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103845367A (en) * | 2014-01-03 | 2014-06-11 | 青岛东海药业有限公司 | Micro-ecological preparation applied to aquaculture of abalones and sea cucumbers |
| CN104000024A (en) * | 2014-06-06 | 2014-08-27 | 威海金牌生物科技股份有限公司 | Micro-ecological preparation for promoting growth of juvenile sea cucumbers and application method |
| CN104686838A (en) * | 2015-02-11 | 2015-06-10 | 青岛根源生物技术集团有限公司 | Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive |
| CN108949615A (en) * | 2018-06-25 | 2018-12-07 | 青岛农业大学 | Probiotics for the apostichopus japonicus Selenka feed that ferments |
| CN112340856A (en) * | 2020-11-03 | 2021-02-09 | 苏州汉风科技发展有限公司 | Biological environment-friendly wastewater treatment method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1579201A (en) * | 2004-05-17 | 2005-02-16 | 陕西省科学院酶工程研究所 | Feed additive containing microbe and biological enzyme |
| CN101690545A (en) * | 2009-09-07 | 2010-04-07 | 肇东市日成酶制剂有限公司 | Method for producing complex micro-ecological preparation with microbial agents and enzyme |
| CN101837118A (en) * | 2009-03-20 | 2010-09-22 | 菏泽普恩药业有限公司 | Compound chicken colibacillosis resisting veterinary drug preparation |
| CN102106488A (en) * | 2011-02-12 | 2011-06-29 | 大连圣贤水产养殖有限公司 | Environment-friendly holothurian compound feed |
| CN102973702A (en) * | 2012-08-23 | 2013-03-20 | 郑州后羿制药有限公司 | Piglet yellow and white scour treatment compound traditional Chinese medicine composition and preparation method thereof |
| CN102972638A (en) * | 2012-12-24 | 2013-03-20 | 黑龙江天予生物科技有限责任公司 | Animal feed additive and preparation method thereof |
| CN103103122A (en) * | 2013-01-31 | 2013-05-15 | 大连海洋大学 | Composite stichopus japonicus growth promotion micro-ecological preparation and feeding method thereof |
| CN103127178A (en) * | 2011-11-28 | 2013-06-05 | 鼎正动物药业（天津）有限公司 | Drug for curing intestinal infection of livestock and preparation method thereof |
-
2013
- 2013-08-09 CN CN201310347575.6A patent/CN103404729B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1579201A (en) * | 2004-05-17 | 2005-02-16 | 陕西省科学院酶工程研究所 | Feed additive containing microbe and biological enzyme |
| CN101837118A (en) * | 2009-03-20 | 2010-09-22 | 菏泽普恩药业有限公司 | Compound chicken colibacillosis resisting veterinary drug preparation |
| CN101690545A (en) * | 2009-09-07 | 2010-04-07 | 肇东市日成酶制剂有限公司 | Method for producing complex micro-ecological preparation with microbial agents and enzyme |
| CN102106488A (en) * | 2011-02-12 | 2011-06-29 | 大连圣贤水产养殖有限公司 | Environment-friendly holothurian compound feed |
| CN103127178A (en) * | 2011-11-28 | 2013-06-05 | 鼎正动物药业（天津）有限公司 | Drug for curing intestinal infection of livestock and preparation method thereof |
| CN102973702A (en) * | 2012-08-23 | 2013-03-20 | 郑州后羿制药有限公司 | Piglet yellow and white scour treatment compound traditional Chinese medicine composition and preparation method thereof |
| CN102972638A (en) * | 2012-12-24 | 2013-03-20 | 黑龙江天予生物科技有限责任公司 | Animal feed additive and preparation method thereof |
| CN103103122A (en) * | 2013-01-31 | 2013-05-15 | 大连海洋大学 | Composite stichopus japonicus growth promotion micro-ecological preparation and feeding method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘铁钢 等: "5种微生态制剂对刺参幼参的生态安全性", 《大连海洋大学学报》 * |
| 李成林 等: "微生态制剂在刺参养殖中的应用与展望", 《齐鲁渔业》 * |
| 胡功政 等: "《新全实用兽药手册》", 30 September 2009, 河南科学技术出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103845367A (en) * | 2014-01-03 | 2014-06-11 | 青岛东海药业有限公司 | Micro-ecological preparation applied to aquaculture of abalones and sea cucumbers |
| CN104000024A (en) * | 2014-06-06 | 2014-08-27 | 威海金牌生物科技股份有限公司 | Micro-ecological preparation for promoting growth of juvenile sea cucumbers and application method |
| CN104686838A (en) * | 2015-02-11 | 2015-06-10 | 青岛根源生物技术集团有限公司 | Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive |
| CN108949615A (en) * | 2018-06-25 | 2018-12-07 | 青岛农业大学 | Probiotics for the apostichopus japonicus Selenka feed that ferments |
| CN112340856A (en) * | 2020-11-03 | 2021-02-09 | 苏州汉风科技发展有限公司 | Biological environment-friendly wastewater treatment method |
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