CN104686838A - Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive - Google Patents
Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive Download PDFInfo
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- CN104686838A CN104686838A CN201510070240.3A CN201510070240A CN104686838A CN 104686838 A CN104686838 A CN 104686838A CN 201510070240 A CN201510070240 A CN 201510070240A CN 104686838 A CN104686838 A CN 104686838A
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Abstract
The invention provides a composite microecological feed additive capable of increasing the freshwater fish growth rate and application of the composite microecological feed additive. The additive comprises the following components: aspergillus niger solid fermentation compound enzyme, bacillus subtilis, bacillus cereus, cellulose, xylanase, neutral protease and phytase. The composite microecological feed additive disclosed by the invention is added with the solid fermentation compound enzyme, so that the utilization rate of the freshwater fish to baits is effectively increased, the growth of fishes is promoted, the breeding of pathogenic bacteria including aeromonas hydrophila, vibrio alginolyticus and the like in a water body can be inhibited due to the addition of fungicides, the occurrence possibility of diseases of saprolegniasis, white skin diseases, bacillary enteritis and the like is reduced; compared with similar additives, the additive disclosed by the invention has the advantages of being safe, high in efficiency, special, free of residues, free of side effects and the like; the composite microecological feed additive can be used for improving and keeping the good growth state of the freshwater fish and effectively increasing the freshwater fish growth rate.
Description
Technical field
The invention belongs to aquatic feeds technical field, relate to feed addictive, be specifically related to a kind of composite microorganism type forage additives and the application thereof that improve fresh-water fishes growth rate.
Background technology
Animal raw materials day by day in short supply, makes the development of aquatic feeds face significant challenge.At present, vegetable raw material is being widely used in aquatic feeds.But freshwater fish, to vegetable raw material digestibility and utilization ability, as freshwater fish lacks phytase, can not make full use of phytate phosphorus etc.; Lacking endogenous non-starch polysaccharide enzyme causes alimentary canal chymeviscosity to increase, and have impact on digesting and assimilating of nutriment.Research shows, the complex enzymes such as usable fibers element enzyme, zytase, phytase carry out enzymolysis, stimulate endogenous digestive ferment to secrete, improve the digestibility of vegetable protein, starch, promote the growth of aquatic livestock.Containing acid protease, cellulase, pectase etc. in aspergillus niger solid fermentation complex enzyme, enzyme system enriches, low production cost is aided with commercial neutral proteinase, cellulase, zytase and phytase again, the deficiency of the digested enzyme of fish digestive tube can be supplemented, and then sufficient enzymolysis is carried out to feed, improve efficiency of feed utilization.By adding bacillus subtilis and bacillus cereus, its breeding in enteron aisle can change fish gastrointestinal micro-flora, improves micro-ecological environment in body, makes beneficial bacterium occupy advantage, suppress the growth and breeding of pathogenic microorganism, reduce and prevent the generation of enterogastric diseases.
Summary of the invention
The present invention is directed to the situation of fresh-water fishes to forage plant property raw material digestion power difference, provide a kind of composite microorganism type forage additives and the application thereof that improve fresh-water fishes growth rate, utilize feed addictive of the present invention can significantly improve the growth rate of fresh-water fishes.
For achieving the above object, the present invention adopts following technical proposals to be achieved:
Improve a composite microorganism type forage additives for fresh-water fishes growth rate, described composite microorganism type forage additives comprises following component:
Described bacillus cereus selects bacterial strain bacillus cereus J19, and its Classification And Nomenclature is bacillus cereus Bacillus cereus, and be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, its deposit number is: CGMCC No.10052.
Described aspergillus niger solid fermentation complex enzyme adopts mass ratio to be that wheat bran and the cotton dregs of 7:3 ferment, and add that to account for the bed material mass ratio that ferments be (the NH of 4.0%
4)
2sO
4, material-water ratio 1:1.2, inoculated aspergillus niger, initial pH 7.5, cultivation temperature 28 DEG C, incubation time 60h, period stirring 2 times.
Further improvement to technique scheme: containing zytase 500 ~ 800U/g in the described aspergillus niger solid fermentation complex enzyme that fermentation ends obtains, mannase 50 ~ 100U/g, cellulase 20 ~ 30U/g, pectase 200 ~ 300U/g, acid protease 2500 ~ 3000U/g.
Further improvement to technique scheme: described micro-ecological additive comprises following component: aspergillus niger solid fermentation complex enzyme content is 30%; Bacillus subtilis bacterial content is 8 × 10
9individual/g; Bacillus cereus is 1 × 10
9individual/g; Cellulase content is 250U/g; Xylanase content is 2500U/g; Neutral proteinase content is 2500U/g; Phytase content is 500U/g.
Present invention also offers the application of described composite microorganism type forage additives in the feed of preparation raising fresh-water fishes growth rate.
Further improvement to technique scheme: the addition of described composite microorganism type forage additives is according to perfect compound feed gross weight 0.5 ‰-1 ‰, is mixed and made into premix by this product and other feed addictive or feedstuff, debugs and granulate.Described fresh-water fishes are carp.
Advantage of the present invention and technique effect are: component major part of the present invention adopts commercial enzyme preparation and bacteria preparation, carry out the deficiency of the endogenous digestive ferment of supplementary fresh-water fishes, and strengthen occupy-place and the bacteria resistance function of beneficial bacterium, to reduce Freshwater Fish-Intestines tract disease occurrence probability and growth promoting effects for target, according to the enzyme-added compound microecological additive of a kind of novel bacterium that fresh-water fishes growth characteristics and feed composition design, can effectively promote that fresh-water fishes grow.Aspergillus niger solid fermentation complex enzyme enzyme system selected by the present invention is abundant, enzyme is lived high, the resistance to elevated temperatures of bacillus subtilis is good, Bacillus cereus strain low temperature germination rate is high, with commercial cellulase, zytase, neutral proteinase, phytase compound, ANFs in vegetable raw material can be reduced, promote the decomposition of high molecular weight protein and carbohydrate, improve the digestibility of fresh-water fishes, build healthy intestinal environment, growth promoting effects.
In water body, add the breeding that described additive can suppress the pathogenic bacteria such as Aeromonas hydrophila in water body, molten algae blood vibrios, reduce the occurrence probability of the diseases such as saprolegniasis, white skin disease, bacillary enteritis; The advantage such as there is security, high efficiency, selectivity, noresidue compared with similar additive and have no side effect; Use described composite microorganism type forage additives to improve and maintain the good growth conditions of fresh-water fishes, effectively improving its growth rate.
Accompanying drawing explanation
Fig. 1 is the screening experiment result of dominant strain in embodiment 1.
Detailed description of the invention
The present invention is described in further detail the present invention in conjunction with following specific embodiment.
The screening of embodiment 1, dominant strain of the present invention
The present embodiment experimental selection 7 bacillus carry out heat-resisting and produce enzyme performance compare, wherein D-11, D-12, D-14 are bacillus licheniformis (Bacillus licheniformis) different sub-strain, K12, K13 are bacillus subtilis (Bacillus subtilis) different sub-strain, be obtainable commercialization bacterium powder on market, bacillus cereus is separated from ginseng culture zone, institute Zhenghai, Haiyang, Rushan City, Weihai City bed mud, and bacillus subtilis is commercially available bacterium powder.
First screen dominant strain with high temperature bath, as shown in Figure 1, after 85 DEG C of water-bath 20min, survival rate is all more than 60%, and wherein bacillus subtilis is best, and survival rate is 100%.Next is that bacterial strain D-12, D-11, D-14 are respectively 95.23%, 93.88%, 89%.When the survival rate of bacillus subtilis, bacillus cereus and D-11 is higher after 95 DEG C of water-bath 20min, be respectively 55.26%, 53.90%, 45.80%.95 DEG C of tolerances of all the other 4 strain bacterium are very poor, all lower than 10%.
Starch, the proteolytic activity of table 17 strain bacterium compare
Experimental result shows, the comprehensive enzymolysis ability of bacillus subtilis to starch and albumen is better than other each bacterial strain.K-12, D-12, bacillus cereus all have certain enzymolysis ability to starch and albumen, between difference not significantly (wax sample >K-12>D-12).D-14 has stronger enzymolysis ability to albumen, and enzymolysis circle is maximum, is better than other all bacterial strain, but this experiment does not find that it has Starch Hydrolysis ability.And D-11, K-13 on culture medium all without hydrolysis circle, to starch and albumen without enzymolysis ability.
Comprehensive analysis: Bacillus cereus strain selected by the present invention is for being separated from ginseng culture zone, institute Zhenghai, Haiyang, Rushan City, Weihai City bed mud in November, 2010, and described bacillus cereus laboratory numbering J19, is bacillus cereus J19.
Described bacillus cereus J19 bacterial strain is carried out culture presevation, the title of depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC); Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.The preservation date of bacillus cereus Bacillus cereus: on November 25th, 2014; Deposit number: CGMCC No.10052.
Embodiment 2
The composite microorganism type forage additives that the present invention improves fresh-water fishes growth rate comprises following component:
Its cellulase, zytase, neutral proteinase and phytase are commercial enzyme preparation, and bacillus subtilis adopts commercially available bacterium powder, described bacillus cereus deposit number: CGMCC No.10052.
Described aspergillus niger solid fermentation complex enzyme, adopt wheat bran and cotton dregs to form fermentation materials and ferment, both mass ratioes are 7:3, and interpolation accounts for the (NH that fermentation materials mass ratio is 4.0%
4)
2sO
4, material-water ratio 1:1.2, bacteria using amount 2*10 is adopted in aspergillus niger inoculation
9the seed liquor of individual/ml, described seed liquor inoculum concentration is 1L/ ton fermentation materials, initial pH 7.5, cultivation temperature 28 DEG C, incubation time 60h, period stirring 2 times, aspergillus niger adopts conventional bacterial classification.Fermentation ends obtains aspergillus niger solid fermentation complex enzyme, wherein zytase 500 ~ 800U/g, mannase 50 ~ 100U/g, cellulase 20 ~ 30U/g, pectase 200 ~ 300U/g, acid protease 2500 ~ 3000U/g.
The impact that embodiment 3, the present invention grow carp fry
The present embodiment experiment is carried out in Jimo national marine research center Chinese Marine University cultivation base, and experiment aquarium volume is 45L, uninterruptedly inflates during cultivation.Experiment is divided into 2 groups, and experimental group adds 5g/kg composite microorganism type forage additives, and control group does not add composite microorganism type forage additives.Each group 7 repetitions, an aquarium is a repetition, and each aquarium puts 8 carps in a suitable place to breed, and specification is 11.93g ± 0.52/, and the test period is that 60d, 30d weigh once.
The impact that table 2 composite microorganism type forage additives grows carp fry
Experimental result is as shown in table 2, through the continuous feeding experiment of 60d, result of the test shows that adding composite microorganism type forage additives can significantly reduce feed coefficient, improve the growth rate of carp, particularly second month, compared with control group, test group rate of body weight gain on average improves 22.14%, and is better than control group by the active degree observing carp.
Composite microorganism type forage additives of the present invention effectively can improve the utilization ratio of fresh-water fishes to bait, promote the growth of fish, suppress the breeding of the pathogenic bacteria such as Aeromonas hydrophila, molten algae blood vibrios in water body, reduce the occurrence probability of the diseases such as saprolegniasis, white skin disease, bacillary enteritis, can improve and maintain the good growth conditions of fresh-water fishes, effectively improving its growth rate.
Above embodiment only in order to technical scheme of the present invention to be described, but not is limited; Although with reference to previous embodiment to invention has been detailed description, for the person of ordinary skill of the art, still can modify to the technical scheme described in previous embodiment, or equivalent replacement is carried out to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (8)
1. improve a composite microorganism type forage additives for fresh-water fishes growth rate, it is characterized in that: described composite microorganism type forage additives comprises following component:
The mass ratio that aspergillus niger solid fermentation complex enzyme accounts for feed addictive is not less than 30%;
Bacillus subtilis is not less than 8 × 10
9individual/g;
Bacillus cereus is not less than 1 × 10
9individual/g;
Cellulase is not less than 250 U/g;
Zytase is not less than 2500 U/g;
Neutral proteinase is not less than 2500 U/g;
Phytase is not less than 500 U/g.
2. the composite microorganism type forage additives of raising fresh-water fishes growth rate according to claim 1, it is characterized in that: described bacillus cereus selects bacterial strain bacillus cereus J19, its Classification And Nomenclature is bacillus cereus
bacillus cereus, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, its deposit number is: CGMCC No.10052.
3. the composite microorganism type forage additives of raising fresh-water fishes growth rate according to claim 2, it is characterized in that: described aspergillus niger solid fermentation complex enzyme adopts mass ratio to be that wheat bran and the cotton dregs of 7:3 ferment, and interpolation accounts for the (NH that fermentation materials mass ratio is 4.0%
4)
2sO
4, material-water ratio 1:1.2, inoculated aspergillus niger, initial pH 7.5, cultivation temperature 28 DEG C, incubation time 60h, period stirring 2 times.
4. the composite microorganism type forage additives of raising fresh-water fishes growth rate according to claim 3, it is characterized in that: containing zytase 500 ~ 800U/g in the described aspergillus niger solid fermentation complex enzyme that fermentation ends obtains, mannase 50 ~ 100U/g, cellulase 20 ~ 30U/g, pectase 200 ~ 300U/g, acid protease 2500 ~ 3000U/g.
5. the composite microorganism type forage additives of raising fresh-water fishes growth rate according to claim 3, is characterized in that: described micro-ecological additive comprises following component: aspergillus niger solid fermentation complex enzyme content is 30%; Bacillus subtilis bacterial content is 8 × 10
9individual/g; Bacillus cereus is 1 × 10
9individual/g; Cellulase content is 250 U/g; Xylanase content is 2500 U/g; Neutral proteinase content is 2500 U/g; Phytase content is 500 U/g.
6. the composite microorganism type forage additives according to any one of claim 1-5 improves the application in the feed of fresh-water fishes growth rate in preparation.
7. composite microorganism type forage additives according to claim 6 improves the application in the feed of fresh-water fishes growth rate in preparation, it is characterized in that: the addition of described composite microorganism type forage additives is according to perfect compound feed gross weight 0.5 ‰-1 ‰, this product and other feed addictive or feedstuff are mixed and made into premix, debug and granulate.
8. composite microorganism type forage additives according to claim 6 improves the application in the feed of fresh-water fishes growth rate in preparation, it is characterized in that: described fresh-water fishes are carp.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106260747A (en) * | 2016-08-09 | 2017-01-04 | 浙江粤海饲料有限公司 | A kind of compound additive preventing and treating fresh-water fishes yellow fat disease and preparation method thereof |
| CN106689796A (en) * | 2015-11-13 | 2017-05-24 | 江苏银宝生物科技有限公司 | Black carp biological feed formula and processing method |
| CN108815200A (en) * | 2018-07-09 | 2018-11-16 | 江西省科学院生物资源研究所 | A kind of bacillus combination preparation and its preparation method and application |
| CN109497281A (en) * | 2018-10-26 | 2019-03-22 | 湖北源清生物科技股份有限公司 | A kind of compound organic feed of microorganism |
| CN110710617A (en) * | 2019-10-11 | 2020-01-21 | 青岛尚德生物技术有限公司 | Fermented soft pellet feed for penaeus vannamei boone and preparation method and application thereof |
| CN113151123A (en) * | 2021-06-17 | 2021-07-23 | 吉林农业大学 | Bacillus cereus for producing cellulase and xylanase at low temperature |
| CN114828866A (en) * | 2019-08-30 | 2022-07-29 | 国际生物资源有限公司 | Feed additive formula for fish and preparation and use methods thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1520739A (en) * | 2002-02-26 | 2004-08-18 | 东莞市豪发生物工程开发有限公司 | Bacilli fungi mixed fermentation for producing microbial formulation and compound enzyme feed additive |
| CN101849618A (en) * | 2010-04-13 | 2010-10-06 | 浙江大学 | Method for producing complex enzyme for feed |
| CN102616941A (en) * | 2012-03-26 | 2012-08-01 | 大连理工大学 | Sea cucumber microecological water quality conditioning agent and method for preparing same |
| CN103349152A (en) * | 2013-07-26 | 2013-10-16 | 何雨豪 | Functional complex microbial agent serving as aquaculture bait and aquaculture method |
| CN103404729A (en) * | 2013-08-09 | 2013-11-27 | 青岛根源生物技术集团有限公司 | Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics |
| CN103695396A (en) * | 2013-12-23 | 2014-04-02 | 湖南鸿鹰生物科技有限公司 | High-activity composite enzyme for feed |
| CN103865854A (en) * | 2014-03-18 | 2014-06-18 | 安徽农业大学 | Composite micro-ecologic preparation and preparation method thereof |
| CN104152386A (en) * | 2014-08-14 | 2014-11-19 | 河北农业大学 | Multifunctional microecological preparation |
-
2015
- 2015-02-11 CN CN201510070240.3A patent/CN104686838A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1520739A (en) * | 2002-02-26 | 2004-08-18 | 东莞市豪发生物工程开发有限公司 | Bacilli fungi mixed fermentation for producing microbial formulation and compound enzyme feed additive |
| CN101849618A (en) * | 2010-04-13 | 2010-10-06 | 浙江大学 | Method for producing complex enzyme for feed |
| CN102616941A (en) * | 2012-03-26 | 2012-08-01 | 大连理工大学 | Sea cucumber microecological water quality conditioning agent and method for preparing same |
| CN103349152A (en) * | 2013-07-26 | 2013-10-16 | 何雨豪 | Functional complex microbial agent serving as aquaculture bait and aquaculture method |
| CN103404729A (en) * | 2013-08-09 | 2013-11-27 | 青岛根源生物技术集团有限公司 | Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics |
| CN103695396A (en) * | 2013-12-23 | 2014-04-02 | 湖南鸿鹰生物科技有限公司 | High-activity composite enzyme for feed |
| CN103865854A (en) * | 2014-03-18 | 2014-06-18 | 安徽农业大学 | Composite micro-ecologic preparation and preparation method thereof |
| CN104152386A (en) * | 2014-08-14 | 2014-11-19 | 河北农业大学 | Multifunctional microecological preparation |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689796A (en) * | 2015-11-13 | 2017-05-24 | 江苏银宝生物科技有限公司 | Black carp biological feed formula and processing method |
| CN106260747A (en) * | 2016-08-09 | 2017-01-04 | 浙江粤海饲料有限公司 | A kind of compound additive preventing and treating fresh-water fishes yellow fat disease and preparation method thereof |
| CN108815200A (en) * | 2018-07-09 | 2018-11-16 | 江西省科学院生物资源研究所 | A kind of bacillus combination preparation and its preparation method and application |
| CN108815200B (en) * | 2018-07-09 | 2020-08-28 | 江西省科学院生物资源研究所 | Bacillus composite preparation and preparation method and application thereof |
| CN109497281A (en) * | 2018-10-26 | 2019-03-22 | 湖北源清生物科技股份有限公司 | A kind of compound organic feed of microorganism |
| CN114828866A (en) * | 2019-08-30 | 2022-07-29 | 国际生物资源有限公司 | Feed additive formula for fish and preparation and use methods thereof |
| CN110710617A (en) * | 2019-10-11 | 2020-01-21 | 青岛尚德生物技术有限公司 | Fermented soft pellet feed for penaeus vannamei boone and preparation method and application thereof |
| CN113151123A (en) * | 2021-06-17 | 2021-07-23 | 吉林农业大学 | Bacillus cereus for producing cellulase and xylanase at low temperature |
| CN113151123B (en) * | 2021-06-17 | 2022-07-08 | 吉林农业大学 | Bacillus cereus for producing cellulase and xylanase at low temperature |
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Application publication date: 20150610 |