CN103103122A - Composite stichopus japonicus growth promotion micro-ecological preparation and feeding method thereof - Google Patents
Composite stichopus japonicus growth promotion micro-ecological preparation and feeding method thereof Download PDFInfo
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- CN103103122A CN103103122A CN2013100364959A CN201310036495A CN103103122A CN 103103122 A CN103103122 A CN 103103122A CN 2013100364959 A CN2013100364959 A CN 2013100364959A CN 201310036495 A CN201310036495 A CN 201310036495A CN 103103122 A CN103103122 A CN 103103122A
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Abstract
The invention discloses a composite stichopus japonicus growth promotion micro-ecological preparation. The micro-ecological preparation is characterized by comprising photosynthetic bacteria, bacillus subtilis, lactic acid bacteria and rhodotorula benthica, wherein the concentration ratio of the photosynthetic bacteria to the bacillus subtilis to the lactic acid bacteria to the rhodotorula benthica is (3-5.5):(3.5-5):(0.5-1.5):(1-1.5); the final concentration of both the photosynthetic bacteria and the bacillus subtilis is (0.5-1.5)*10<4>cfu/ml; and the final concentration of both the lactic acid bacteria and the rhodotorula benthica is (2-4)*10<3>cfu/ml. When in use, 7-10 days are used as one cycle, the four micro-ecological preparations with the above concentration ratio are diluted by 10-15 times by water, are mixed uniformly and then are uniformly spilled into culture water respectively, in the second day of the primary feeding operation, the preparations are spilled with the amount that is a half of the feeding amount of the first day, and the proper water temperature for feeding is 15DEG C to 28 DEG C. The micro-ecological preparation for the healthy culture of stichopus japonicus is easy to get, significant in effect and relatively low in cost.
Description
Technical field
The present invention relates to a kind of ecological agent, particularly the put-on method of a kind of built-up type stichopus japonicus growth promotion probiotics and this kind preparation.
Background technology
Along with the continuous expansion of China's aquaculture scale, excess load cultivation, indiscriminate row is put excessively, and the microbiotic phenomenon such as exceed standard highlights day by day.Keep ecotope healthy, implementing the green health cultivation has become and has been related to the whether key issue of Sustainable development of culture fishery.And the appearance of probiotics is walked the green health aquaculture model for aquaculture possibility is provided.Probiotics is the microbial cells that will screen from physical environment, through cultivating the active bacteria formulation that contains a large amount of probioticss of making after breeding.Do not contain any pollutent and chemical nuisance in probiotics, to improving water quality, improving surviving rate and relative growth rate, reduce sickness rate, improve the fishery products quality, obvious effect is arranged.
Probiotics divides single bacterium probiotics and complex microorganism preparations by the bacterial classification constituted mode, and the former is that a kind of single culture consists of, and latter is that many bacterial classifications mix formation.The bacterial classification that uses in probiotics at present is more, and China's approved uses mainly contains the 6 large classes such as genus bacillus, lactobacillus, streptococcus faecium, yeast, bacteriophagic Bdellovibrio and bacteroides fragilis.At present, the product of a lot of probioticses is arranged on market, ichthyosauru mixes pearl, and very few for the probiotics of stichopus japonicus healthy aquaculture.
Summary of the invention
The present invention is in order to solve the existing above-mentioned deficiency of prior art, to propose a kind of acquisition easy, successful, the probiotics that lower-cost stichopus japonicus healthy aquaculture is used.
Technical solution of the present invention is: a kind of built-up type stichopus japonicus growth promotion probiotics, and it is characterized in that: described preparation comprises photosynthetic bacterium, genus bacillus, milk-acid bacteria and ocean rhodotorula, and their concentration ratio is:
Photosynthetic bacterium: genus bacillus: milk-acid bacteria: ocean rhodotorula=3-5.5:3.5-5:0.5-1.5:1-1.5,
And wherein the viable bacteria final concentration of photosynthetic bacterium and genus bacillus is 0.5-1.5 * 10
4Cfu/mL, the viable bacteria final concentration of milk-acid bacteria and ocean rhodotorula is 2-4 * 10
3Cfu/mL.
A kind of put-on method of built-up type stichopus japonicus growth promotion probiotics as above, it is characterized in that: take 7-10 days as the cycle, get four kinds of probioticses of above-mentioned concentration ratio, dilute with water 10-15 doubly respectively, evenly splash in aquaculture water respectively after mixing, and at the first second day of throwing in operation, again splash according to half of first day injected volume, the suitable water temperature of input is 15-28 ℃.
The present invention compares with prior art, has following advantage:
The bacterium liquid of various bacterium used in the present invention obtains easily, and its price is comparatively cheap, can effectively control aquaculture cost.And probiotics configuration of the present invention is simple, easy to operate, is applicable to the apostichopus japonicus culture of various different situations.This kind probiotics can obviously improve condition of water quality simultaneously, reaches the purpose of the growth that promotes aquatic products economic animal (stichopus japonicus), and can reduce the objectionable impurities such as ammonia-state nitrogen, nitrite nitrogen in water body, successful.Therefore can say that it has possessed multiple advantage, be particularly suitable for applying in aquaculture field, its market outlook are very wide.
Embodiment
The below will illustrate the specific embodiment of the present invention.
A kind of built-up type stichopus japonicus growth promotion probiotics, it comprises photosynthetic bacterium, genus bacillus, milk-acid bacteria and four kinds of bacterial classifications of ocean rhodotorula, and the concentration ratio of these four kinds of bacterial classifications is:
Photosynthetic bacterium: genus bacillus: milk-acid bacteria: ocean rhodotorula=3-5.5:3.5-5:0.5-1.5:1-1.5,
And wherein the viable bacteria final concentration of photosynthetic bacterium and genus bacillus is 0.5-1.5 * 10
4Cfu/mL, the viable bacteria final concentration of milk-acid bacteria and ocean rhodotorula is 2-4 * 10
3Cfu/mL.
Carry out splashing of this kind probiotics take 7-10 days as one-period.Namely at first get four kinds of bacterial classifications according to above-mentioned concentration ratio, and respectively dilute with water 10-15 doubly, splashes evenly in aquaculture water after mixing fully, the second day after first the input is splashed again according to half of first day injected volume; After this after 7-10 days, still get four kinds of bacterial classifications according to above-mentioned concentration ratio, after distinguishing dilute with water 10-15 times, fully mix and splash in aquaculture water.And when this probiotics is thrown in, suitable water temperature should be 15-28 ℃.
Detect proof, this kind probiotics can obviously improve condition of water quality, uses 6-8 all after date, and the ammonia nitrogen content in aquaculture water reduces by 24.48%, and nitrite nitrogen reduces by 5.47%; It can also work to promote the stichopus japonicus growth simultaneously, and the day weight gain of stichopus japonicus can improve 15-20% than control group.
Claims (2)
1. built-up type stichopus japonicus growth promotion probiotics, it is characterized in that: described preparation comprises photosynthetic bacterium, genus bacillus, milk-acid bacteria and ocean rhodotorula, and their concentration ratio is:
Photosynthetic bacterium: genus bacillus: milk-acid bacteria: ocean rhodotorula=3-5.5:3.5-5:0.5-1.5:1-1.5,
And wherein the viable bacteria final concentration of photosynthetic bacterium and genus bacillus is 0.5-1.5 * 10
4Cfu/mL, the viable bacteria final concentration of milk-acid bacteria and ocean rhodotorula is 2-4 * 10
3Cfu/mL.
2. the put-on method of a built-up type stichopus japonicus growth promotion probiotics as claimed in claim 1, it is characterized in that: take 7-10 days as the cycle, get four kinds of probioticses of above-mentioned concentration ratio, dilute with water 10-15 doubly respectively, evenly splash in aquaculture water respectively after mixing, and at the first second day of throwing in operation, again splash according to half of first day injected volume, the suitable water temperature of input is 15-28 ℃.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103404716A (en) * | 2013-07-09 | 2013-11-27 | 青岛中浩环能技术开发有限公司 | Compound microorganism bacterium powder and preparation method thereof |
CN103404729A (en) * | 2013-08-09 | 2013-11-27 | 青岛根源生物技术集团有限公司 | Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics |
CN103478044A (en) * | 2013-09-29 | 2014-01-01 | 大连海洋大学 | Method for culturing apostichopusjaponicus juveniles by Indian bacillus |
CN108949615A (en) * | 2018-06-25 | 2018-12-07 | 青岛农业大学 | Probiotics for the apostichopus japonicus Selenka feed that ferments |
CN109055266A (en) * | 2018-08-20 | 2018-12-21 | 广州海诚生物科技有限公司 | A kind of biological agent and the preparation method and application thereof with vibrios growth in the inhibition shrimp pool |
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CN101082027A (en) * | 2006-05-31 | 2007-12-05 | 上海泓宝绿色水产科技发展有限公司 | Preparation of holothurian-fertilizing bacterium and method for restoring sea cucumber cultivation pool environment |
CN101912051A (en) * | 2010-07-13 | 2010-12-15 | 大连海洋大学 | Fermentation process of sea cucumber compound feed |
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Patent Citations (2)
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CN101082027A (en) * | 2006-05-31 | 2007-12-05 | 上海泓宝绿色水产科技发展有限公司 | Preparation of holothurian-fertilizing bacterium and method for restoring sea cucumber cultivation pool environment |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103404716A (en) * | 2013-07-09 | 2013-11-27 | 青岛中浩环能技术开发有限公司 | Compound microorganism bacterium powder and preparation method thereof |
CN103404716B (en) * | 2013-07-09 | 2015-08-19 | 青岛中浩环能技术开发有限公司 | Compound microorganism bacterium powder and preparation method |
CN103404729A (en) * | 2013-08-09 | 2013-11-27 | 青岛根源生物技术集团有限公司 | Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics |
CN103404729B (en) * | 2013-08-09 | 2015-02-04 | 青岛根源生物技术集团有限公司 | Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics |
CN103478044A (en) * | 2013-09-29 | 2014-01-01 | 大连海洋大学 | Method for culturing apostichopusjaponicus juveniles by Indian bacillus |
CN108949615A (en) * | 2018-06-25 | 2018-12-07 | 青岛农业大学 | Probiotics for the apostichopus japonicus Selenka feed that ferments |
CN109055266A (en) * | 2018-08-20 | 2018-12-21 | 广州海诚生物科技有限公司 | A kind of biological agent and the preparation method and application thereof with vibrios growth in the inhibition shrimp pool |
CN109055266B (en) * | 2018-08-20 | 2021-10-01 | 广州海诚生物科技有限公司 | Biological agent capable of inhibiting growth of vibrio in shrimp pond and preparation method and application thereof |
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Application publication date: 20130515 |